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data-collection-position="0" data-entity-id="13168570" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/13168570/Genome_analysis_of_Cronobacter_phage_vB_CsaP_Ss1_reveals_an_endolysin_with_potential_for_biocontrol_of_Gram_negative_bacterial_pathogens">Genome analysis of Cronobacter phage vB_CsaP_Ss1 reveals an endolysin with potential for biocontrol of Gram-negative bacterial pathogens</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32423144" href="https://independent.academia.edu/HorstNeve">Horst Neve</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of General Virology, 2014</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genome analysis of Cronobacter phage vB_CsaP_Ss1 reveals an endolysin with potential for biocontrol of Gram-negative bacterial pathogens","attachmentId":45618798,"attachmentType":"pdf","work_url":"https://www.academia.edu/13168570/Genome_analysis_of_Cronobacter_phage_vB_CsaP_Ss1_reveals_an_endolysin_with_potential_for_biocontrol_of_Gram_negative_bacterial_pathogens","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/13168570/Genome_analysis_of_Cronobacter_phage_vB_CsaP_Ss1_reveals_an_endolysin_with_potential_for_biocontrol_of_Gram_negative_bacterial_pathogens"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="60774117" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/60774117/Phage_S144_a_New_Polyvalent_Phage_Infecting_Salmonella_spp_and_Cronobacter_sakazakii">Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="74004580" href="https://independent.academia.edu/MichelaGambino1">Michela Gambino</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Molecular Sciences</p><p class="ds-related-work--abstract ds2-5-body-sm">Phages are generally considered species- or even strain-specific, yet polyvalent phages are able to infect bacteria from different genera. Here, we characterize the novel polyvalent phage S144, a member of the Loughboroughvirus genus. By screening 211 Enterobacteriaceae strains, we found that phage S144 forms plaques on specific serovars of Salmonella enterica subsp. enterica and on Cronobacter sakazakii. Analysis of phage resistant mutants suggests that the O-antigen of lipopolysaccharide is the phage receptor in both bacterial genera. The S144 genome consists of 53,628 bp and encodes 80 open reading frames (ORFs), but no tRNA genes. In total, 32 ORFs coding for structural proteins were confirmed by ESI-MS/MS analysis, whereas 45 gene products were functionally annotated within DNA metabolism, packaging, nucleotide biosynthesis and phage morphogenesis. Transmission electron microscopy showed that phage S144 is a myovirus, with a prolate head and short tail fibers. The putative S144...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii","attachmentId":74068475,"attachmentType":"pdf","work_url":"https://www.academia.edu/60774117/Phage_S144_a_New_Polyvalent_Phage_Infecting_Salmonella_spp_and_Cronobacter_sakazakii","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/60774117/Phage_S144_a_New_Polyvalent_Phage_Infecting_Salmonella_spp_and_Cronobacter_sakazakii"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="52853661" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/52853661/Molecular_Characterization_of_Cronobacter_Lipopolysaccharide_O_Antigen_Gene_Clusters_and_Development_of_Serotype_Specific_PCR_Assays">Molecular Characterization of Cronobacter Lipopolysaccharide O-Antigen Gene Clusters and Development of Serotype-Specific PCR Assays</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32839525" href="https://independent.academia.edu/VenugopalSathyamoorthy">Venugopal Sathyamoorthy</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Applied and Environmental Microbiology, 2011</p><p class="ds-related-work--abstract ds2-5-body-sm">ABSTRACTCronobacter(formerlyEnterobacter sakazakii) is a recently defined genus consisting of six species,C. sakazakii,C. malonaticus,C. dublinensis,C. muytjensii,C. turicensis, andCronobactergenomospecies 1. In this study, MboII restriction fragment length polymorphism (RFLP) patterns of O-antigen gene clusters, located betweengalFandgnd, were used to identify serotypes inCronobacterspp. Seven O-antigen RFLP clusters were generated, including threeC. sakazakiiclusters, previously identified as serotypes O1, O2, and O3. The O-antigen regions of six strains with unique RFLP patterns, including twoC. sakazakiistrains, twoC. malonaticusstrains, oneC. turicensisstrain, and oneC. muytjensiistrain, revealed three O-antigen gene clusters shared amongCronobacterspecies. PCR assays were developed, targeting thewzxO-antigen polymerase gene, and used to screen 231Cronobacterstrains to determine the frequency of these newly identified serotypes.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Molecular Characterization of Cronobacter Lipopolysaccharide O-Antigen Gene Clusters and Development of Serotype-Specific PCR Assays","attachmentId":69916990,"attachmentType":"pdf","work_url":"https://www.academia.edu/52853661/Molecular_Characterization_of_Cronobacter_Lipopolysaccharide_O_Antigen_Gene_Clusters_and_Development_of_Serotype_Specific_PCR_Assays","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/52853661/Molecular_Characterization_of_Cronobacter_Lipopolysaccharide_O_Antigen_Gene_Clusters_and_Development_of_Serotype_Specific_PCR_Assays"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="73569205" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/73569205/Complete_genome_sequence_of_the_siphoviral_bacteriophage_%CE%92%CF%86_R3177_which_lyses_an_OXA_66_producing_carbapenem_resistant_Acinetobacter_baumannii_isolate">Complete genome sequence of the siphoviral bacteriophage Βϕ-R3177, which lyses an OXA-66-producing carbapenem-resistant Acinetobacter baumannii isolate</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2799617" href="https://kribb.academia.edu/ChoongMinRyu">Choong-Min Ryu</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Archives of virology, 2015</p><p class="ds-related-work--abstract ds2-5-body-sm">In recent years, antimicrobial resistance has become a major medical threat worldwide. Among these threats, the rapid increase in carbapenem-resistant Acinetobacter baumannii (CRAB) is a particularly challenging global issue in the health care setting. In this study, a novel lytic A. baumannii phage, Βϕ-R3177, infecting carbapenem-resistant A. baumannii strains was isolated from sewage samples at a hospital. The morphology of the phage as assessed by transmission electron microscopy (TEM) indicated that it belongs to the family Siphoviridae within the order Caudovirales. It has a linear double-stranded DNA genome of 47,575 bp with a G+C content of 39.83 %. Eighty open reading frames (ORFs) were predicted; however, only 14 ORFs were annotated as encoding functional proteins, while most of the ORFs encoded hypothetical proteins. Among the total ORFs of the phage genome, no toxin-related genes were detected. A bioinformatics analysis showed that the whole genome sequence of phage Βϕ-R3...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Complete genome sequence of the siphoviral bacteriophage Βϕ-R3177, which lyses an OXA-66-producing carbapenem-resistant Acinetobacter baumannii isolate","attachmentId":82040343,"attachmentType":"pdf","work_url":"https://www.academia.edu/73569205/Complete_genome_sequence_of_the_siphoviral_bacteriophage_%CE%92%CF%86_R3177_which_lyses_an_OXA_66_producing_carbapenem_resistant_Acinetobacter_baumannii_isolate","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/73569205/Complete_genome_sequence_of_the_siphoviral_bacteriophage_%CE%92%CF%86_R3177_which_lyses_an_OXA_66_producing_carbapenem_resistant_Acinetobacter_baumannii_isolate"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="91739754" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/91739754/Genetic_Analysis_of_the_Cronobacter_sakazakii_O4_to_O7_O_Antigen_Gene_Clusters_and_Development_of_a_PCR_Assay_for_Identification_of_All_C_sakazakii_O_Serotypes">Genetic Analysis of the Cronobacter sakazakii O4 to O7 O-Antigen Gene Clusters and Development of a PCR Assay for Identification of All C. sakazakii O Serotypes</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="247488260" href="https://independent.academia.edu/CindyHe16">Cindy He</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Applied and Environmental Microbiology, 2012</p><p class="ds-related-work--abstract ds2-5-body-sm">The Gram-negative bacterium Cronobacter sakazakii is an emerging food-borne pathogen that causes severe invasive infections in neonates. Variation in the O-antigen lipopolysaccharide in the outer membrane provides the basis for Gram-negative bacteria serotyping. The O-antigen serotyping scheme for C. sakazakii , which includes seven serotypes (O1 to O7), has been recently established, and the O-antigen gene clusters and specific primers for three C. sakazakii serotypes (O1, O2, and O3) have been characterized. In this study, the C. sakazakii O4, O5, O6, and O7 O-antigen gene clusters were sequenced, and gene functions were predicted on the basis of homology. C. sakazakii O4 shared a similar O-antigen gene cluster with Escherichia coli O103. The general features and anomalies of all seven C. sakazakii O-antigen gene clusters were evaluated and the relationship between O-antigen structures and their gene clusters were investigated. Serotype-specific genes for O4 to O7 were identified,...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genetic Analysis of the Cronobacter sakazakii O4 to O7 O-Antigen Gene Clusters and Development of a PCR Assay for Identification of All C. sakazakii O Serotypes","attachmentId":94940444,"attachmentType":"pdf","work_url":"https://www.academia.edu/91739754/Genetic_Analysis_of_the_Cronobacter_sakazakii_O4_to_O7_O_Antigen_Gene_Clusters_and_Development_of_a_PCR_Assay_for_Identification_of_All_C_sakazakii_O_Serotypes","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/91739754/Genetic_Analysis_of_the_Cronobacter_sakazakii_O4_to_O7_O_Antigen_Gene_Clusters_and_Development_of_a_PCR_Assay_for_Identification_of_All_C_sakazakii_O_Serotypes"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="70601468" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/70601468/Isolation_of_lytic_bacteriophages_and_their_potential_to_control_Cronobacter_spp_opportunistic_food_borne_pathogens">Isolation of lytic bacteriophages and their potential to control Cronobacter spp. - opportunistic food-borne pathogens</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="98296350" href="https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi">Dr. Agustin Krisna W.,STP,M.Si</a></div><p class="ds-related-work--metadata ds2-5-body-xs">IOP Conference Series: Earth and Environmental Science</p><p class="ds-related-work--abstract ds2-5-body-sm">Bacteriophage could be an effective and safe method to decrease the presence of pathogens in food. In this study, lytic phages were isolated and examined for their efficacy to control pathogens. It was isolated using the “plaque assay” method with the indicator bacteria, Cronobacter spp. and the lytic ability of isolated phage was confirmed using turbidity test. The isolated φKA5 from chicken skin samples was able to inhibit the growth of bacterial host (Cronobacter spp.) specifically Cronobacter malonaticus strain 05CHPL53. Infection of Cronobacter malonaticus using φKA5 for 360 minutes showed the significant reduction of Cronobacter growth. Based on morphological classification with the ICTV system, the φKA5 can be classified into the Myoviridae family with a contractile tail. These data suggested that isolated phage from raw chicken skin are potential agents for controlling Cronobacter spp.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Isolation of lytic bacteriophages and their potential to control Cronobacter spp. - opportunistic food-borne pathogens","attachmentId":80285103,"attachmentType":"pdf","work_url":"https://www.academia.edu/70601468/Isolation_of_lytic_bacteriophages_and_their_potential_to_control_Cronobacter_spp_opportunistic_food_borne_pathogens","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" 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class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="8279562" href="https://chonbuk.academia.edu/DiliniChandrapala">Dilini Chandrapala</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Infection and Immunity, 2014</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Putative Inv Is Essential for Basolateral Invasion of Caco-2 Cells and Acts Synergistically with OmpA To Affect In Vitro and In Vivo Virulence of Cronobacter sakazakii ATCC 29544","attachmentId":77490236,"attachmentType":"pdf","work_url":"https://www.academia.edu/66207767/Putative_Inv_Is_Essential_for_Basolateral_Invasion_of_Caco_2_Cells_and_Acts_Synergistically_with_OmpA_To_Affect_In_Vitro_and_In_Vivo_Virulence_of_Cronobacter_sakazakii_ATCC_29544","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" 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