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Mizanur Milon | University of Dhaka, Bangladesh - Academia.edu
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class="affiliations-container fake-truncate js-profile-affiliations"><div><a class="u-tcGrayDarker" href="https://univdhaka.academia.edu/">University of Dhaka, Bangladesh</a>, <a class="u-tcGrayDarker" href="https://univdhaka.academia.edu/Departments/Microbiology/Documents">Microbiology</a>, <span class="u-tcGrayDarker">Graduate Student</span></div></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="Mizanur" data-follow-user-id="4716963" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" 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u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="4716963" href="https://www.academia.edu/Documents/in/Microbiology"><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://univdhaka.academia.edu/MizanurMilon","location":"/MizanurMilon","scheme":"https","host":"univdhaka.academia.edu","port":null,"pathname":"/MizanurMilon","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Microbiology"]}" data-trace="false" data-dom-id="Pill-react-component-748880c5-d75d-428d-9a16-e95e961e30ec"></div> <div 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<div id="Pill-react-component-ad529cd9-a391-46ac-8cd2-74b488879f81"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="4716963" href="https://www.academia.edu/Documents/in/Probiotics"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Probiotics"]}" data-trace="false" data-dom-id="Pill-react-component-4d7bd1c5-c84b-430c-9f0f-ef975f615b01"></div> <div id="Pill-react-component-4d7bd1c5-c84b-430c-9f0f-ef975f615b01"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="4716963" href="https://www.academia.edu/Documents/in/Fish_Nutrition"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Fish Nutrition"]}" data-trace="false" data-dom-id="Pill-react-component-8823b53c-8b6e-4f83-8ac0-46a0f0ab66b2"></div> <div 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href="https://www.academia.edu/78641594/Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_Food"><img alt="Research paper thumbnail of Occurrence of Enterobacteriaceae with Serological Cross Reactivity towards Salmonella spp., Shigella spp. and Vibrio cholerae in Food" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/78641594/Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_Food">Occurrence of Enterobacteriaceae with Serological Cross Reactivity towards Salmonella spp., Shigella spp. and Vibrio cholerae in Food</a></div><div class="wp-workCard_item"><span>British Microbiology Research Journal</span><span>, 2015</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="78641594"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="78641594"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 78641594; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); 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class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/10136762/Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food"><img alt="Research paper thumbnail of Sress tolerant virulent strains of Cronobacter sakazakii from food" class="work-thumbnail" src="https://attachments.academia-assets.com/36248961/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/10136762/Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food">Sress tolerant virulent strains of Cronobacter sakazakii from food</a></div><div class="wp-workCard_item"><span>Biological Research</span><span>, Nov 25, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract Background: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract <br />Background: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was <br />to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. <br />Result: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical <br />characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. <br />specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately <br />high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates <br />were capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression, <br />haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant <br />resistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them <br />produced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with <br />other gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri <br />and Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress. <br />Conclusion: Special attention should be given in ensuring hygiene in production and post-processing to prevent <br />contamination of food with such stress-tolerant virulent Cronobacter sakazakii. <br />Keywords: Cronobacter, Food, Virulent, Bangladesh</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fb9a0d311bec54878ee78678d7e0485f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":36248961,"asset_id":10136762,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/36248961/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="10136762"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="10136762"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 10136762; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=10136762]").text(description); $(".js-view-count[data-work-id=10136762]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 10136762; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='10136762']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 10136762, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fb9a0d311bec54878ee78678d7e0485f" } } $('.js-work-strip[data-work-id=10136762]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":10136762,"title":"Sress tolerant virulent strains of Cronobacter sakazakii from food","translated_title":"","metadata":{"abstract":"Abstract\r\nBackground: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was\r\nto isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh.\r\nResult: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical\r\ncharacteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp.\r\nspecific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately\r\nhigh antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates\r\nwere capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression,\r\nhaemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant\r\nresistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them\r\nproduced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with\r\nother gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri\r\nand Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress.\r\nConclusion: Special attention should be given in ensuring hygiene in production and post-processing to prevent\r\ncontamination of food with such stress-tolerant virulent Cronobacter sakazakii.\r\nKeywords: Cronobacter, Food, Virulent, Bangladesh","publication_date":{"day":25,"month":11,"year":2014,"errors":{}},"publication_name":"Biological Research"},"translated_abstract":"Abstract\r\nBackground: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was\r\nto isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh.\r\nResult: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical\r\ncharacteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp.\r\nspecific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately\r\nhigh antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates\r\nwere capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression,\r\nhaemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant\r\nresistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them\r\nproduced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with\r\nother gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri\r\nand Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress.\r\nConclusion: Special attention should be given in ensuring hygiene in production and post-processing to prevent\r\ncontamination of food with such stress-tolerant virulent Cronobacter sakazakii.\r\nKeywords: Cronobacter, Food, Virulent, Bangladesh","internal_url":"https://www.academia.edu/10136762/Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food","translated_internal_url":"","created_at":"2015-01-12T23:54:51.765-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":4716963,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":36248961,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/36248961/thumbnails/1.jpg","file_name":"Cronobacter_sakazakii_Paper-bmc.pdf","download_url":"https://www.academia.edu/attachments/36248961/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Sress_tolerant_virulent_strains_of_Crono.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/36248961/Cronobacter_sakazakii_Paper-bmc-libre.pdf?1421137424=\u0026response-content-disposition=attachment%3B+filename%3DSress_tolerant_virulent_strains_of_Crono.pdf\u0026Expires=1732849425\u0026Signature=DCOhMNpHyFsm-RpWqUArnQcI3I7-1Q-noMmQpaJB~3FhPWys3GvGx1gJTYiQQNQTLTgUbQ8TGbvvw8t09uQUmbwespoxDzIi6-4AEsyVb5RAN~HO0~f-1EUwZ6PW9hGQhLEjFEG~rysUyfK3GxTc~XoWIhaEwXcaqP0e6WuVlSjHesdW8lnoW6j3TPo3c5GS1cnO8yhIMqZxA-ikoxs3u4qM586PUsZLxUgWZrTratwekNkIOFjBOGV09nMMiKczqwSFBnMgBY49tT4ajDK2QF1u3z5ijkHGvCMobd7l4jOlhR~5GfQOlWpmTCckU6ED4e7O9T492uoT0-S84qRjUg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food","translated_slug":"","page_count":12,"language":"en","content_type":"Work","owner":{"id":4716963,"first_name":"Mizanur","middle_initials":null,"last_name":"Milon","page_name":"MizanurMilon","domain_name":"univdhaka","created_at":"2013-07-01T18:18:13.239-07:00","display_name":"Mizanur Milon","url":"https://univdhaka.academia.edu/MizanurMilon"},"attachments":[{"id":36248961,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/36248961/thumbnails/1.jpg","file_name":"Cronobacter_sakazakii_Paper-bmc.pdf","download_url":"https://www.academia.edu/attachments/36248961/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Sress_tolerant_virulent_strains_of_Crono.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/36248961/Cronobacter_sakazakii_Paper-bmc-libre.pdf?1421137424=\u0026response-content-disposition=attachment%3B+filename%3DSress_tolerant_virulent_strains_of_Crono.pdf\u0026Expires=1732849425\u0026Signature=DCOhMNpHyFsm-RpWqUArnQcI3I7-1Q-noMmQpaJB~3FhPWys3GvGx1gJTYiQQNQTLTgUbQ8TGbvvw8t09uQUmbwespoxDzIi6-4AEsyVb5RAN~HO0~f-1EUwZ6PW9hGQhLEjFEG~rysUyfK3GxTc~XoWIhaEwXcaqP0e6WuVlSjHesdW8lnoW6j3TPo3c5GS1cnO8yhIMqZxA-ikoxs3u4qM586PUsZLxUgWZrTratwekNkIOFjBOGV09nMMiKczqwSFBnMgBY49tT4ajDK2QF1u3z5ijkHGvCMobd7l4jOlhR~5GfQOlWpmTCckU6ED4e7O9T492uoT0-S84qRjUg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="8464737"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/8464737/Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_food"><img alt="Research paper thumbnail of Occurrence of Enterobacteriaceae with Serological Cross Reactivity towards Salmonella spp., Shigella spp. and Vibrio cholerae in food" class="work-thumbnail" src="https://attachments.academia-assets.com/34850790/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/8464737/Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_food">Occurrence of Enterobacteriaceae with Serological Cross Reactivity towards Salmonella spp., Shigella spp. and Vibrio cholerae in food</a></div><div class="wp-workCard_item"><span>Scciencedomain international</span><span>, Sep 9, 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="50d75f91b856b85eaa14664e32790e90" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":34850790,"asset_id":8464737,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/34850790/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="8464737"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="8464737"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 8464737; 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Place and Duration of Study: This study was conducted in Industrial Microbiology Laboratory, IFST, BCSIR, Dhaka, Bangladesh during January-November, 2013. Methodology: 14 Enterobacteriaceae was isolated from food samples and identified. Serological reaction was determined by slide agglutination method. Results: Among 14 isolates, 6 were identified to be Cronobacter spp., 3 to be Shigella spp., 2 to be Enterobacter spp., 2 to be Escherichia coli and 1 as Klebsiella spp. Three of the isolates belonging to Cronobacter showed extremely strong cross-reactivity with Salmonella spp., Shigella spp. and Vibrio cholerae. Other isolates gave relatively weak but significant cross-reactions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of cell surface protein also showed similar bands in the isolates as type strains of Salmonella, Shigella and Vibrio cholerae.","publication_date":{"day":9,"month":9,"year":2014,"errors":{}},"publication_name":"Scciencedomain international","grobid_abstract_attachment_id":34850790},"translated_abstract":null,"internal_url":"https://www.academia.edu/8464737/Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_food","translated_internal_url":"","created_at":"2014-09-23T19:32:04.135-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":4716963,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":34850790,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/34850790/thumbnails/1.jpg","file_name":"Mizan_paper_on_cross_reactivity.pdf","download_url":"https://www.academia.edu/attachments/34850790/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Occurrence_of_Enterobacteriaceae_with_Se.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/34850790/Mizan_paper_on_cross_reactivity-libre.pdf?1411527763=\u0026response-content-disposition=attachment%3B+filename%3DOccurrence_of_Enterobacteriaceae_with_Se.pdf\u0026Expires=1732849425\u0026Signature=UPZAD6Sa450B4SowDtmVUVXhW2gQWQ86yALpqIRx4cyDfPHJ7xxQf8ATMlIExRFnIMoQprYCfl5r233etJw43S5Pzfv3YSK6T5xT-0aVS-7-rO-~uHVCkAPtGcf-lbcgqpQDRt1FQ1UfAQoiMw8I1-8px81H~4YI0In5OVo9DqgS2bk5r-W7k2ifmeH2yTqhfpJCk2W9~SsRMU-F2uSTkQEriPeRch-X~SkPwx~zNRWwL~oh7FCkSyyjnsoigLtVCo0~yjMu6XfQldjSrDwOPYt6hzuLCODofJ1ISarm0mv3lzkPmy6vnhYZ0wi4jomGzC4-yBA5tEOdKiopzY98gA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_food","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":4716963,"first_name":"Mizanur","middle_initials":null,"last_name":"Milon","page_name":"MizanurMilon","domain_name":"univdhaka","created_at":"2013-07-01T18:18:13.239-07:00","display_name":"Mizanur Milon","url":"https://univdhaka.academia.edu/MizanurMilon"},"attachments":[{"id":34850790,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/34850790/thumbnails/1.jpg","file_name":"Mizan_paper_on_cross_reactivity.pdf","download_url":"https://www.academia.edu/attachments/34850790/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Occurrence_of_Enterobacteriaceae_with_Se.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/34850790/Mizan_paper_on_cross_reactivity-libre.pdf?1411527763=\u0026response-content-disposition=attachment%3B+filename%3DOccurrence_of_Enterobacteriaceae_with_Se.pdf\u0026Expires=1732849425\u0026Signature=UPZAD6Sa450B4SowDtmVUVXhW2gQWQ86yALpqIRx4cyDfPHJ7xxQf8ATMlIExRFnIMoQprYCfl5r233etJw43S5Pzfv3YSK6T5xT-0aVS-7-rO-~uHVCkAPtGcf-lbcgqpQDRt1FQ1UfAQoiMw8I1-8px81H~4YI0In5OVo9DqgS2bk5r-W7k2ifmeH2yTqhfpJCk2W9~SsRMU-F2uSTkQEriPeRch-X~SkPwx~zNRWwL~oh7FCkSyyjnsoigLtVCo0~yjMu6XfQldjSrDwOPYt6hzuLCODofJ1ISarm0mv3lzkPmy6vnhYZ0wi4jomGzC4-yBA5tEOdKiopzY98gA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="5604123"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/5604123/Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh"><img alt="Research paper thumbnail of Antimicrobial resistance and virulence factors of enterobacteriaceae isolated from food samples of Bangladesh" class="work-thumbnail" src="https://attachments.academia-assets.com/32680895/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/5604123/Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh">Antimicrobial resistance and virulence factors of enterobacteriaceae isolated from food samples of Bangladesh</a></div><div class="wp-workCard_item"><span>International Journal of Microbiology and Immunology Research</span><span>, Jan 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4b66532473b4247caaf8bf1fe0e01a2e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":32680895,"asset_id":5604123,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/32680895/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="5604123"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="5604123"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 5604123; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=5604123]").text(description); $(".js-view-count[data-work-id=5604123]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 5604123; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='5604123']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 5604123, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4b66532473b4247caaf8bf1fe0e01a2e" } } $('.js-work-strip[data-work-id=5604123]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":5604123,"title":"Antimicrobial resistance and virulence factors of enterobacteriaceae isolated from food samples of Bangladesh","translated_title":"","metadata":{"grobid_abstract":"Enterobacteriaceae (Klebsiella, Enterobacter Shigella, Escherichia and Citrobacter) are pathogens responsible for a wide range of infections. The aim of this study was to determine the antimicrobial resistance and virulence properties of Enterobacteriaceae in food samples of Bangladesh. Fortyfood samples such as milk powder, horlicks, honey, chocolates and biscuits were included. Five genera of Enterobacteriaceae including Shigella, Escherichia, Klebsiella, Enterobacter and Citrobacter were found in 12 among 40 food samples. Among 12 isolates, two Klebsiella, three Enterobacter, three Citrobacter, three Shigella and one Escherichia were identified on the basis of biochemical tests and Carbohydrate utilization assay according to Bergey's Manual of Systematic Bacteriology. These strains were found to have moderately high antibiotic resistance against vancomycin, nitrofurantoin, penicillin G, imipenem, ampicillin adnd neomycin. These isolates can be considered as virulent as they demonstrated many virulence properties. Most of the isolates were capable of producing extracellular protease, curli expression, haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant resistance to human serum, can tolerate high concentration of salt and bile. This study concludes that some of the food products in Bangladesh are contaminated with pathogenic Enterobacteriaceae and thus special attention should be given in ensuring hygiene in production and post-processing.","publication_date":{"day":null,"month":1,"year":2014,"errors":{}},"publication_name":"International Journal of Microbiology and Immunology Research","grobid_abstract_attachment_id":32680895},"translated_abstract":null,"internal_url":"https://www.academia.edu/5604123/Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh","translated_internal_url":"","created_at":"2014-01-04T13:39:48.591-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":4716963,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":32680895,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/32680895/thumbnails/1.jpg","file_name":"Fakruddin_et_al.pdf","download_url":"https://www.academia.edu/attachments/32680895/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_resistance_and_virulence_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/32680895/Fakruddin_et_al-libre.pdf?1391212884=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_resistance_and_virulence_f.pdf\u0026Expires=1732849425\u0026Signature=bqOTfvHlmPxyc5-8~YGQ9CiuqpoLQCts-A9KMAcEPxRAaNttlbgydDyLhAe4UXvZFZVOd85iLNeLyrUJOdE4iVlmEowbq-GhPWPmZFQQULFmeybN6L5D3cEp4fCRsjh1OMstEE4elSY1~OonIpEWo4ocweuYtJ5n11uPq~vk9TpcYlISIRJjIJ4htcukv00Zy8fOaPc5Z~Mnf5HAknRCEiEXm3OUOTXRykGY1N2Vo2ZTolFnrF3XQle-vDowz~hnH4jO76vSBkGB76ttqz3MesMVNgeBCJ7N8qWc9lBhz43ouiXJUxQx-Np8zVIy43F6SlrQ~2YpmVKXKrYX6m-c4A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh","translated_slug":"","page_count":7,"language":"en","content_type":"Work","owner":{"id":4716963,"first_name":"Mizanur","middle_initials":null,"last_name":"Milon","page_name":"MizanurMilon","domain_name":"univdhaka","created_at":"2013-07-01T18:18:13.239-07:00","display_name":"Mizanur Milon","url":"https://univdhaka.academia.edu/MizanurMilon"},"attachments":[{"id":32680895,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/32680895/thumbnails/1.jpg","file_name":"Fakruddin_et_al.pdf","download_url":"https://www.academia.edu/attachments/32680895/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_resistance_and_virulence_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/32680895/Fakruddin_et_al-libre.pdf?1391212884=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_resistance_and_virulence_f.pdf\u0026Expires=1732849425\u0026Signature=bqOTfvHlmPxyc5-8~YGQ9CiuqpoLQCts-A9KMAcEPxRAaNttlbgydDyLhAe4UXvZFZVOd85iLNeLyrUJOdE4iVlmEowbq-GhPWPmZFQQULFmeybN6L5D3cEp4fCRsjh1OMstEE4elSY1~OonIpEWo4ocweuYtJ5n11uPq~vk9TpcYlISIRJjIJ4htcukv00Zy8fOaPc5Z~Mnf5HAknRCEiEXm3OUOTXRykGY1N2Vo2ZTolFnrF3XQle-vDowz~hnH4jO76vSBkGB76ttqz3MesMVNgeBCJ7N8qWc9lBhz43ouiXJUxQx-Np8zVIy43F6SlrQ~2YpmVKXKrYX6m-c4A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="3992509"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/3992509/Cronobacter_sakazakii_Enterobacter_sakazakii_An_Emerging_Foodborne_Pathogen"><img alt="Research paper thumbnail of Cronobacter sakazakii (Enterobacter sakazakii): An Emerging Foodborne Pathogen" class="work-thumbnail" src="https://attachments.academia-assets.com/31545636/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/3992509/Cronobacter_sakazakii_Enterobacter_sakazakii_An_Emerging_Foodborne_Pathogen">Cronobacter sakazakii (Enterobacter sakazakii): An Emerging Foodborne Pathogen</a></div><div class="wp-workCard_item"><span>International Journal of Biomedical and Advance Research</span><span>, Jul 2013</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0df3fac3e28bfda3992b0e4488bf10cf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":31545636,"asset_id":3992509,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/31545636/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="3992509"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="3992509"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 3992509; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0df3fac3e28bfda3992b0e4488bf10cf" } } $('.js-work-strip[data-work-id=3992509]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":3992509,"title":"Cronobacter sakazakii (Enterobacter sakazakii): An Emerging Foodborne Pathogen","translated_title":"","metadata":{"grobid_abstract":"Cronobacter sakazakii (Enterobacter sakazakii) is an emerging pathogen that causes necrotizing enterocolitis, sepsis, and meningitis. This bacterium is opportunistic pathogen and is linked with life-threatening infections in neonates and elderly persons. It is considered as ubiquitous organism and can be found in a broad range of foods \u0026 food ingredients and in water, in a variety of areas, including hospitals and houses though outbreaks most commonly associated with the ingestion of contaminated powdered infant formula (PIF). International Commission on Microbiological Specifications for Foods has ranked C. sakazakii a \"severe hazard for restricted populations.\" It poses high tolerance to environmental stresses such as osmotic stress, elevated temperature etc. and decontamination processes and has broad antibiotic resistance and resistance to bile salts and disinfectants. Cronobacter sakazakii may survive in macrophage cells and efficiently attach to and invade epithelial cell lines, produce exopolysaccharide, form biofilm and has active efflux pumps. Controlling the organism in the production environment, thereby reducing dissemination, necessitates the provision of suitable diagnostic tools. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="10136762"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/10136762/Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food"><img alt="Research paper thumbnail of Sress tolerant virulent strains of Cronobacter sakazakii from food" class="work-thumbnail" src="https://attachments.academia-assets.com/36248961/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/10136762/Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food">Sress tolerant virulent strains of Cronobacter sakazakii from food</a></div><div class="wp-workCard_item"><span>Biological Research</span><span>, Nov 25, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract Background: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract <br />Background: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was <br />to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. <br />Result: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical <br />characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. <br />specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately <br />high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates <br />were capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression, <br />haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant <br />resistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them <br />produced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with <br />other gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri <br />and Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress. <br />Conclusion: Special attention should be given in ensuring hygiene in production and post-processing to prevent <br />contamination of food with such stress-tolerant virulent Cronobacter sakazakii. <br />Keywords: Cronobacter, Food, Virulent, Bangladesh</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fb9a0d311bec54878ee78678d7e0485f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":36248961,"asset_id":10136762,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/36248961/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="10136762"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="10136762"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 10136762; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=10136762]").text(description); $(".js-view-count[data-work-id=10136762]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 10136762; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='10136762']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 10136762, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fb9a0d311bec54878ee78678d7e0485f" } } $('.js-work-strip[data-work-id=10136762]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":10136762,"title":"Sress tolerant virulent strains of Cronobacter sakazakii from food","translated_title":"","metadata":{"abstract":"Abstract\r\nBackground: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was\r\nto isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh.\r\nResult: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical\r\ncharacteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp.\r\nspecific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately\r\nhigh antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates\r\nwere capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression,\r\nhaemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant\r\nresistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them\r\nproduced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with\r\nother gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri\r\nand Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress.\r\nConclusion: Special attention should be given in ensuring hygiene in production and post-processing to prevent\r\ncontamination of food with such stress-tolerant virulent Cronobacter sakazakii.\r\nKeywords: Cronobacter, Food, Virulent, Bangladesh","publication_date":{"day":25,"month":11,"year":2014,"errors":{}},"publication_name":"Biological Research"},"translated_abstract":"Abstract\r\nBackground: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was\r\nto isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh.\r\nResult: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical\r\ncharacteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp.\r\nspecific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately\r\nhigh antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates\r\nwere capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression,\r\nhaemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant\r\nresistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them\r\nproduced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with\r\nother gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri\r\nand Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress.\r\nConclusion: Special attention should be given in ensuring hygiene in production and post-processing to prevent\r\ncontamination of food with such stress-tolerant virulent Cronobacter sakazakii.\r\nKeywords: Cronobacter, Food, Virulent, Bangladesh","internal_url":"https://www.academia.edu/10136762/Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food","translated_internal_url":"","created_at":"2015-01-12T23:54:51.765-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":4716963,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":36248961,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/36248961/thumbnails/1.jpg","file_name":"Cronobacter_sakazakii_Paper-bmc.pdf","download_url":"https://www.academia.edu/attachments/36248961/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Sress_tolerant_virulent_strains_of_Crono.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/36248961/Cronobacter_sakazakii_Paper-bmc-libre.pdf?1421137424=\u0026response-content-disposition=attachment%3B+filename%3DSress_tolerant_virulent_strains_of_Crono.pdf\u0026Expires=1732849425\u0026Signature=DCOhMNpHyFsm-RpWqUArnQcI3I7-1Q-noMmQpaJB~3FhPWys3GvGx1gJTYiQQNQTLTgUbQ8TGbvvw8t09uQUmbwespoxDzIi6-4AEsyVb5RAN~HO0~f-1EUwZ6PW9hGQhLEjFEG~rysUyfK3GxTc~XoWIhaEwXcaqP0e6WuVlSjHesdW8lnoW6j3TPo3c5GS1cnO8yhIMqZxA-ikoxs3u4qM586PUsZLxUgWZrTratwekNkIOFjBOGV09nMMiKczqwSFBnMgBY49tT4ajDK2QF1u3z5ijkHGvCMobd7l4jOlhR~5GfQOlWpmTCckU6ED4e7O9T492uoT0-S84qRjUg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Sress_tolerant_virulent_strains_of_Cronobacter_sakazakii_from_food","translated_slug":"","page_count":12,"language":"en","content_type":"Work","owner":{"id":4716963,"first_name":"Mizanur","middle_initials":null,"last_name":"Milon","page_name":"MizanurMilon","domain_name":"univdhaka","created_at":"2013-07-01T18:18:13.239-07:00","display_name":"Mizanur Milon","url":"https://univdhaka.academia.edu/MizanurMilon"},"attachments":[{"id":36248961,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/36248961/thumbnails/1.jpg","file_name":"Cronobacter_sakazakii_Paper-bmc.pdf","download_url":"https://www.academia.edu/attachments/36248961/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Sress_tolerant_virulent_strains_of_Crono.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/36248961/Cronobacter_sakazakii_Paper-bmc-libre.pdf?1421137424=\u0026response-content-disposition=attachment%3B+filename%3DSress_tolerant_virulent_strains_of_Crono.pdf\u0026Expires=1732849425\u0026Signature=DCOhMNpHyFsm-RpWqUArnQcI3I7-1Q-noMmQpaJB~3FhPWys3GvGx1gJTYiQQNQTLTgUbQ8TGbvvw8t09uQUmbwespoxDzIi6-4AEsyVb5RAN~HO0~f-1EUwZ6PW9hGQhLEjFEG~rysUyfK3GxTc~XoWIhaEwXcaqP0e6WuVlSjHesdW8lnoW6j3TPo3c5GS1cnO8yhIMqZxA-ikoxs3u4qM586PUsZLxUgWZrTratwekNkIOFjBOGV09nMMiKczqwSFBnMgBY49tT4ajDK2QF1u3z5ijkHGvCMobd7l4jOlhR~5GfQOlWpmTCckU6ED4e7O9T492uoT0-S84qRjUg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology"},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology"},{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology"}],"urls":[]}, dispatcherData: dispatcherData }); 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Place and Duration of Study: This study was conducted in Industrial Microbiology Laboratory, IFST, BCSIR, Dhaka, Bangladesh during January-November, 2013. Methodology: 14 Enterobacteriaceae was isolated from food samples and identified. Serological reaction was determined by slide agglutination method. Results: Among 14 isolates, 6 were identified to be Cronobacter spp., 3 to be Shigella spp., 2 to be Enterobacter spp., 2 to be Escherichia coli and 1 as Klebsiella spp. Three of the isolates belonging to Cronobacter showed extremely strong cross-reactivity with Salmonella spp., Shigella spp. and Vibrio cholerae. Other isolates gave relatively weak but significant cross-reactions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of cell surface protein also showed similar bands in the isolates as type strains of Salmonella, Shigella and Vibrio cholerae.","publication_date":{"day":9,"month":9,"year":2014,"errors":{}},"publication_name":"Scciencedomain international","grobid_abstract_attachment_id":34850790},"translated_abstract":null,"internal_url":"https://www.academia.edu/8464737/Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_food","translated_internal_url":"","created_at":"2014-09-23T19:32:04.135-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":4716963,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":34850790,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/34850790/thumbnails/1.jpg","file_name":"Mizan_paper_on_cross_reactivity.pdf","download_url":"https://www.academia.edu/attachments/34850790/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Occurrence_of_Enterobacteriaceae_with_Se.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/34850790/Mizan_paper_on_cross_reactivity-libre.pdf?1411527763=\u0026response-content-disposition=attachment%3B+filename%3DOccurrence_of_Enterobacteriaceae_with_Se.pdf\u0026Expires=1732849425\u0026Signature=UPZAD6Sa450B4SowDtmVUVXhW2gQWQ86yALpqIRx4cyDfPHJ7xxQf8ATMlIExRFnIMoQprYCfl5r233etJw43S5Pzfv3YSK6T5xT-0aVS-7-rO-~uHVCkAPtGcf-lbcgqpQDRt1FQ1UfAQoiMw8I1-8px81H~4YI0In5OVo9DqgS2bk5r-W7k2ifmeH2yTqhfpJCk2W9~SsRMU-F2uSTkQEriPeRch-X~SkPwx~zNRWwL~oh7FCkSyyjnsoigLtVCo0~yjMu6XfQldjSrDwOPYt6hzuLCODofJ1ISarm0mv3lzkPmy6vnhYZ0wi4jomGzC4-yBA5tEOdKiopzY98gA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Occurrence_of_Enterobacteriaceae_with_Serological_Cross_Reactivity_towards_Salmonella_spp_Shigella_spp_and_Vibrio_cholerae_in_food","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":4716963,"first_name":"Mizanur","middle_initials":null,"last_name":"Milon","page_name":"MizanurMilon","domain_name":"univdhaka","created_at":"2013-07-01T18:18:13.239-07:00","display_name":"Mizanur Milon","url":"https://univdhaka.academia.edu/MizanurMilon"},"attachments":[{"id":34850790,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/34850790/thumbnails/1.jpg","file_name":"Mizan_paper_on_cross_reactivity.pdf","download_url":"https://www.academia.edu/attachments/34850790/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Occurrence_of_Enterobacteriaceae_with_Se.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/34850790/Mizan_paper_on_cross_reactivity-libre.pdf?1411527763=\u0026response-content-disposition=attachment%3B+filename%3DOccurrence_of_Enterobacteriaceae_with_Se.pdf\u0026Expires=1732849425\u0026Signature=UPZAD6Sa450B4SowDtmVUVXhW2gQWQ86yALpqIRx4cyDfPHJ7xxQf8ATMlIExRFnIMoQprYCfl5r233etJw43S5Pzfv3YSK6T5xT-0aVS-7-rO-~uHVCkAPtGcf-lbcgqpQDRt1FQ1UfAQoiMw8I1-8px81H~4YI0In5OVo9DqgS2bk5r-W7k2ifmeH2yTqhfpJCk2W9~SsRMU-F2uSTkQEriPeRch-X~SkPwx~zNRWwL~oh7FCkSyyjnsoigLtVCo0~yjMu6XfQldjSrDwOPYt6hzuLCODofJ1ISarm0mv3lzkPmy6vnhYZ0wi4jomGzC4-yBA5tEOdKiopzY98gA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="5604123"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/5604123/Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh"><img alt="Research paper thumbnail of Antimicrobial resistance and virulence factors of enterobacteriaceae isolated from food samples of Bangladesh" class="work-thumbnail" src="https://attachments.academia-assets.com/32680895/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/5604123/Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh">Antimicrobial resistance and virulence factors of enterobacteriaceae isolated from food samples of Bangladesh</a></div><div class="wp-workCard_item"><span>International Journal of Microbiology and Immunology Research</span><span>, Jan 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4b66532473b4247caaf8bf1fe0e01a2e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":32680895,"asset_id":5604123,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/32680895/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="5604123"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="5604123"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 5604123; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=5604123]").text(description); $(".js-view-count[data-work-id=5604123]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 5604123; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='5604123']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 5604123, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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The aim of this study was to determine the antimicrobial resistance and virulence properties of Enterobacteriaceae in food samples of Bangladesh. Fortyfood samples such as milk powder, horlicks, honey, chocolates and biscuits were included. Five genera of Enterobacteriaceae including Shigella, Escherichia, Klebsiella, Enterobacter and Citrobacter were found in 12 among 40 food samples. Among 12 isolates, two Klebsiella, three Enterobacter, three Citrobacter, three Shigella and one Escherichia were identified on the basis of biochemical tests and Carbohydrate utilization assay according to Bergey's Manual of Systematic Bacteriology. These strains were found to have moderately high antibiotic resistance against vancomycin, nitrofurantoin, penicillin G, imipenem, ampicillin adnd neomycin. These isolates can be considered as virulent as they demonstrated many virulence properties. Most of the isolates were capable of producing extracellular protease, curli expression, haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant resistance to human serum, can tolerate high concentration of salt and bile. This study concludes that some of the food products in Bangladesh are contaminated with pathogenic Enterobacteriaceae and thus special attention should be given in ensuring hygiene in production and post-processing.","publication_date":{"day":null,"month":1,"year":2014,"errors":{}},"publication_name":"International Journal of Microbiology and Immunology Research","grobid_abstract_attachment_id":32680895},"translated_abstract":null,"internal_url":"https://www.academia.edu/5604123/Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh","translated_internal_url":"","created_at":"2014-01-04T13:39:48.591-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":4716963,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":32680895,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/32680895/thumbnails/1.jpg","file_name":"Fakruddin_et_al.pdf","download_url":"https://www.academia.edu/attachments/32680895/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_resistance_and_virulence_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/32680895/Fakruddin_et_al-libre.pdf?1391212884=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_resistance_and_virulence_f.pdf\u0026Expires=1732849425\u0026Signature=bqOTfvHlmPxyc5-8~YGQ9CiuqpoLQCts-A9KMAcEPxRAaNttlbgydDyLhAe4UXvZFZVOd85iLNeLyrUJOdE4iVlmEowbq-GhPWPmZFQQULFmeybN6L5D3cEp4fCRsjh1OMstEE4elSY1~OonIpEWo4ocweuYtJ5n11uPq~vk9TpcYlISIRJjIJ4htcukv00Zy8fOaPc5Z~Mnf5HAknRCEiEXm3OUOTXRykGY1N2Vo2ZTolFnrF3XQle-vDowz~hnH4jO76vSBkGB76ttqz3MesMVNgeBCJ7N8qWc9lBhz43ouiXJUxQx-Np8zVIy43F6SlrQ~2YpmVKXKrYX6m-c4A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Antimicrobial_resistance_and_virulence_factors_of_enterobacteriaceae_isolated_from_food_samples_of_Bangladesh","translated_slug":"","page_count":7,"language":"en","content_type":"Work","owner":{"id":4716963,"first_name":"Mizanur","middle_initials":null,"last_name":"Milon","page_name":"MizanurMilon","domain_name":"univdhaka","created_at":"2013-07-01T18:18:13.239-07:00","display_name":"Mizanur Milon","url":"https://univdhaka.academia.edu/MizanurMilon"},"attachments":[{"id":32680895,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/32680895/thumbnails/1.jpg","file_name":"Fakruddin_et_al.pdf","download_url":"https://www.academia.edu/attachments/32680895/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Antimicrobial_resistance_and_virulence_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/32680895/Fakruddin_et_al-libre.pdf?1391212884=\u0026response-content-disposition=attachment%3B+filename%3DAntimicrobial_resistance_and_virulence_f.pdf\u0026Expires=1732849425\u0026Signature=bqOTfvHlmPxyc5-8~YGQ9CiuqpoLQCts-A9KMAcEPxRAaNttlbgydDyLhAe4UXvZFZVOd85iLNeLyrUJOdE4iVlmEowbq-GhPWPmZFQQULFmeybN6L5D3cEp4fCRsjh1OMstEE4elSY1~OonIpEWo4ocweuYtJ5n11uPq~vk9TpcYlISIRJjIJ4htcukv00Zy8fOaPc5Z~Mnf5HAknRCEiEXm3OUOTXRykGY1N2Vo2ZTolFnrF3XQle-vDowz~hnH4jO76vSBkGB76ttqz3MesMVNgeBCJ7N8qWc9lBhz43ouiXJUxQx-Np8zVIy43F6SlrQ~2YpmVKXKrYX6m-c4A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="3992509"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/3992509/Cronobacter_sakazakii_Enterobacter_sakazakii_An_Emerging_Foodborne_Pathogen"><img alt="Research paper thumbnail of Cronobacter sakazakii (Enterobacter sakazakii): An Emerging Foodborne Pathogen" class="work-thumbnail" src="https://attachments.academia-assets.com/31545636/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/3992509/Cronobacter_sakazakii_Enterobacter_sakazakii_An_Emerging_Foodborne_Pathogen">Cronobacter sakazakii (Enterobacter sakazakii): An Emerging Foodborne Pathogen</a></div><div class="wp-workCard_item"><span>International Journal of Biomedical and Advance Research</span><span>, Jul 2013</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0df3fac3e28bfda3992b0e4488bf10cf" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":31545636,"asset_id":3992509,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/31545636/download_file?st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&st=MTczMjg0NTgyNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="3992509"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="3992509"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 3992509; 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This bacterium is opportunistic pathogen and is linked with life-threatening infections in neonates and elderly persons. It is considered as ubiquitous organism and can be found in a broad range of foods \u0026 food ingredients and in water, in a variety of areas, including hospitals and houses though outbreaks most commonly associated with the ingestion of contaminated powdered infant formula (PIF). International Commission on Microbiological Specifications for Foods has ranked C. sakazakii a \"severe hazard for restricted populations.\" It poses high tolerance to environmental stresses such as osmotic stress, elevated temperature etc. and decontamination processes and has broad antibiotic resistance and resistance to bile salts and disinfectants. Cronobacter sakazakii may survive in macrophage cells and efficiently attach to and invade epithelial cell lines, produce exopolysaccharide, form biofilm and has active efflux pumps. Controlling the organism in the production environment, thereby reducing dissemination, necessitates the provision of suitable diagnostic tools. 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