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class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Dr. Agustin Krisna W.,STP,M.Si</h3></div><div class="js-work-strip profile--work_container" data-work-id="125584812"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/125584812/Produksi_Etanol_dari_Tetes_Tebu_oleh_Saccharomyces_cerevisiae_Pembentuk_Flok_NRRL_Y_265_"><img alt="Research paper thumbnail of Produksi Etanol dari Tetes Tebu oleh Saccharomyces cerevisiae Pembentuk Flok (NRRL – Y 265)" class="work-thumbnail" src="https://attachments.academia-assets.com/119601236/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a 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})(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "432e9a50ba8222bffc87410c6767b797" } } $('.js-work-strip[data-work-id=123412569]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123412569,"title":"Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"The energy crisis triggers the use of energy sources that are renewable, such as biomass made from lignocellulosic materials, to produce various chemical compounds for food ingredients and biofuel. The efficient conversion of lignocellulosic biomass into products with added value involves the activity of microorganisms, such as yeasts. For the conversion, microorganisms must be able to use various sugars in lignocellulosic biomass, including pentose sugars, especially xylose. This study aims to isolate xylose-utilizing yeasts and analyze their fermentation activity to produce xylitol and ethanol, as well as their ability to grow in liquid hydrolysate produced from pretreated lignocellulosic biomass. Nineteen yeast isolates could grow on solid and liquid media using solely xylose as a carbon source. All isolates can grow in a xylose medium with incubation at 30 °C, 37 °C, 42 °C, and 45 °C. Six isolates, namely SLI (1), SL3, SL6, SL7, R5, and OPT4B, were chosen based on their considerable growth and high xylose consumption rate in a medium with 50 g/L xylose with incubation at 30 °C for 48 h. Four isolates tested, namely SLI (1), SL6, SL7, and R5, can produce xylitol in media containing xylose carbon sources. The concentration of xylitol produced was determined using highpressure liquid chromatography (HPLC), and the results ranged from 5.0 to 6.0 g/L. Five isolates tested, namely SLI (1), SL6, SL3, R5, and OPT4B, can produce ethanol. The ethanol content produced was determined using gas chromatography (GC), with concentrations ranging from 0.85 to 1.34 g/L. Three isolates, namely SL1(1), R5, and SL6, were able to produce xylitol and ethanol from xylose as carbon sources and were also able to grow on liquid hydrolyzate from pretreated oil palm trunk waste with the subcritical water method. The three isolates were further analyzed using the 18S rDNA sequence to identify the species and confirm their phylogenetic position. Identification based on DNA sequence analysis revealed that isolates SL1(1) and R5 were Pichia kudriavzevii, while isolate SL6 was Candida xylopsoci. The yeast strains isolated from this study could potentially be used for the bioconversion process of lignocellulosic biomass waste to produce value-added derivative products.","publication_date":{"day":12,"month":10,"year":2023,"errors":{}},"publication_name":"Bioresources and 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Science","url":"https://www.academia.edu/Documents/in/Food_Science"},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":56291,"name":"Ethanol Fuel","url":"https://www.academia.edu/Documents/in/Ethanol_Fuel"},{"id":71540,"name":"Xylitol","url":"https://www.academia.edu/Documents/in/Xylitol"},{"id":93647,"name":"Biofuel","url":"https://www.academia.edu/Documents/in/Biofuel"},{"id":131405,"name":"Hydrolysate","url":"https://www.academia.edu/Documents/in/Hydrolysate"},{"id":151659,"name":"Yeast","url":"https://www.academia.edu/Documents/in/Yeast"},{"id":269129,"name":"Fermentation","url":"https://www.academia.edu/Documents/in/Fermentation"},{"id":614751,"name":"Xylose","url":"https://www.academia.edu/Documents/in/Xylose"},{"id":1030794,"name":"Hydrolysis","url":"https://www.academia.edu/Documents/in/Hydrolysis"},{"id":3369889,"name":"Lignocellulosic Biomass","url":"https://www.academia.edu/Documents/in/Lignocellulosic_Biomass"}],"urls":[{"id":44369401,"url":"https://bioresourcesbioprocessing.springeropen.com/counter/pdf/10.1186/s40643-023-00691-y"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040855"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040855/In_Silico_Analysis_of_Active_Compounds_from_Herbal_Plants_Against_Penicillin_Binding_Protein_2a_PBP2a_of_Methicillin_Resistant_Staphylococcus_Aureus"><img alt="Research paper thumbnail of In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus" class="work-thumbnail" src="https://attachments.academia-assets.com/114516802/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040855/In_Silico_Analysis_of_Active_Compounds_from_Herbal_Plants_Against_Penicillin_Binding_Protein_2a_PBP2a_of_Methicillin_Resistant_Staphylococcus_Aureus">In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Herbal plants are frequently used for medication by people as they contain rich bioactive compoun...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. In sum, it suggested three compounds with the best potential ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="593da6a1b204b0a7aa0382c01397aea1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":114516802,"asset_id":119040855,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/114516802/download_file?st=MTczMjUzMTI3Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040855"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040855"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040855; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040855]").text(description); $(".js-view-count[data-work-id=119040855]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040855; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040855']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040855, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "593da6a1b204b0a7aa0382c01397aea1" } } $('.js-work-strip[data-work-id=119040855]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040855,"title":"In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus","translated_title":"","metadata":{"abstract":"Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. In sum, it suggested three compounds with the best potential ...","publisher":"Research Square Platform LLC"},"translated_abstract":"Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040853"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040853/The_increasement_of_rice_straw_hydrolysis_using_blend_crude_cellulose_enzyme_from_Trichoderma_reesei_and_Aspergillus_niger"><img alt="Research paper thumbnail of The increasement of rice straw hydrolysis using blend crude cellulose enzyme from Trichoderma reesei and Aspergillus niger" class="work-thumbnail" src="https://attachments.academia-assets.com/114516771/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040853/The_increasement_of_rice_straw_hydrolysis_using_blend_crude_cellulose_enzyme_from_Trichoderma_reesei_and_Aspergillus_niger">The increasement of rice straw hydrolysis using blend crude cellulose enzyme from Trichoderma reesei and Aspergillus niger</a></div><div class="wp-workCard_item"><span>Research and reviews in biosciences</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. The use...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. The use of two crude enzyme separately only able to produce a low levels of glucose, but after the two crude enzyme being mixed, it is able to improve the yield of glucose on cellulose hydrolysis process by using rice straw as substrate which has undergone pretreatment by alkali microwave. The combination of crude enzyme from Trichoderma reesei and Aspergillus niger (2:1) has the FP-ase activity of 1,002 IU/ml, CMC-ase activity of 2.23 IU/ml and â-glucosidase activity of 0.168 IU/ml. The activity of the crude enzyme cellulose combination are able to generate sugar at 12.89 mg/ml in the process of hydrolysis for 72 hours.  2014 Trade Science Inc. - INDIA</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9e8daa9de93d204a91a01bcdf5a54ef7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":114516771,"asset_id":119040853,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/114516771/download_file?st=MTczMjUzMTI3Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040853"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040853"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040853; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040853]").text(description); $(".js-view-count[data-work-id=119040853]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040853; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040853']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040853, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9e8daa9de93d204a91a01bcdf5a54ef7" } } $('.js-work-strip[data-work-id=119040853]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040853,"title":"The increasement of rice straw hydrolysis using blend crude cellulose enzyme from Trichoderma reesei and Aspergillus niger","translated_title":"","metadata":{"abstract":"The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040852"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040852/The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F"><img alt="Research paper thumbnail of The effect of different substrates on chitinase activity from Bacillus sp. WS4F" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040852/The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F">The effect of different substrates on chitinase activity from Bacillus sp. WS4F</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for plant diseases caused by pathogenic fungi. Bacillus sp. WS4F has chitinase activity which can inhibit the growth of Ganoderma boninense, a fungus that attacks oil palm and causes basal stem rot (BSR). This study aims to investigate the effect of different substrates on the activity of the chitinase from Bacillus sp. WS4F. Two kinds of substrates i.e. chitin flakes and shrimp shells were used in this study. Enzyme activity of chitinase was analyzed after partial purification of enzyme was performed using ammonium sulfate precipitation followed by dialysis. The highest activity of chitinase was achieved by the substrate using shrimp shells. The ammonium sulfate precipitation (60-80% saturation) 0.0949 U/mL for activity enzyme and 0.2639 mg/mL for protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040852"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040852"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040852; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040852]").text(description); $(".js-view-count[data-work-id=119040852]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040852; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040852']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040852, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=119040852]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040852,"title":"The effect of different substrates on chitinase activity from Bacillus sp. 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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.","publisher":"IOP Publishing","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"IOP Conference Series: Earth and Environmental Science"},"translated_abstract":"One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for plant diseases caused by pathogenic fungi. Bacillus sp. WS4F has chitinase activity which can inhibit the growth of Ganoderma boninense, a fungus that attacks oil palm and causes basal stem rot (BSR). This study aims to investigate the effect of different substrates on the activity of the chitinase from Bacillus sp. WS4F. Two kinds of substrates i.e. chitin flakes and shrimp shells were used in this study. Enzyme activity of chitinase was analyzed after partial purification of enzyme was performed using ammonium sulfate precipitation followed by dialysis. The highest activity of chitinase was achieved by the substrate using shrimp shells. The ammonium sulfate precipitation (60-80% saturation) 0.0949 U/mL for activity enzyme and 0.2639 mg/mL for protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.","internal_url":"https://www.academia.edu/119040852/The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F","translated_internal_url":"","created_at":"2024-05-13T19:48:56.413-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[],"research_interests":[{"id":498,"name":"Physics","url":"https://www.academia.edu/Documents/in/Physics"},{"id":529713,"name":"Chitinase","url":"https://www.academia.edu/Documents/in/Chitinase"},{"id":954401,"name":"Enzyme Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Assay"},{"id":1169238,"name":"Chitin","url":"https://www.academia.edu/Documents/in/Chitin"},{"id":1363689,"name":"Ammonium Sulfate","url":"https://www.academia.edu/Documents/in/Ammonium_Sulfate"},{"id":2639492,"name":"IOP conference series-MSE","url":"https://www.academia.edu/Documents/in/IOP_conference_series-MSE"}],"urls":[{"id":41928631,"url":"https://iopscience.iop.org/article/10.1088/1755-1315/924/1/012034/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040851"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/119040851/Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka"><img alt="Research paper thumbnail of Produksi Protein Sel Tunggal Dari Biomasa Mikroorganisme : Kajian Pustaka" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/119040851/Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka">Produksi Protein Sel Tunggal Dari Biomasa Mikroorganisme : Kajian Pustaka</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040851"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040851"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040851; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040851]").text(description); $(".js-view-count[data-work-id=119040851]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040851; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040851']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040851, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=119040851]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040851,"title":"Produksi Protein Sel Tunggal Dari Biomasa Mikroorganisme : Kajian Pustaka","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2015,"errors":{}}},"translated_abstract":null,"internal_url":"https://www.academia.edu/119040851/Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka","translated_internal_url":"","created_at":"2024-05-13T19:48:56.272-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka","translated_slug":"","page_count":null,"language":"id","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040850"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040850/Isolation_screening_and_identification_of_potential_cellulolytic_and_xylanolytic_mold_from_oil_palm_waste"><img alt="Research paper thumbnail of Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste" class="work-thumbnail" src="https://attachments.academia-assets.com/114516801/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040850/Isolation_screening_and_identification_of_potential_cellulolytic_and_xylanolytic_mold_from_oil_palm_waste">Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The objective of this research was to isolate, screen, and identify the cellulase and xylanase-pr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The objective of this research was to isolate, screen, and identify the cellulase and xylanase-producing mold from oil palm waste. There are thirty-two isolates from oil palm waste which are able to degrade and grow on media containing cellulose or xylan as a sole carbon source. Screening to determine cellulolytic and xylanolytic activity was performed by paper disc diffusion method using Congo Red as an indicator. All the thirty-two mold isolates showed cellulolytic and xylanolytic activity with relative enzyme activity (ratio of hydrolysed zone diameter and diameter of colony growth) ranging from 1.04 to 1.62. Based on the macro- and micromorphology characteristics, these isolates were identified as genus Trichoderma, Aspergillus, Rhizopus, Tallaromyces and Penicillium, with the number isolates in each genus was 22, 4, 3, 2, and 1 respectively. The highest cellulolytic and xylanolytic activity was achieved from the isolate namely OPT1(4) which was identified as Talaromyces pinophi...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="260fa1b1d1f91df1b097153f2680ce62" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":114516801,"asset_id":119040850,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/114516801/download_file?st=MTczMjUzMTI3Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040850"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040850"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040850; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040850]").text(description); $(".js-view-count[data-work-id=119040850]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040850; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040850']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040850, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "260fa1b1d1f91df1b097153f2680ce62" } } $('.js-work-strip[data-work-id=119040850]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040850,"title":"Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste","translated_title":"","metadata":{"abstract":"The objective of this research was to isolate, screen, and identify the cellulase and xylanase-producing mold from oil palm waste. 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cultivation medium" class="work-thumbnail" src="https://attachments.academia-assets.com/114516767/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040849/Single_cell_protein_production_of_Chlorella_sp_using_food_processing_waste_as_a_cultivation_medium">Single cell protein production of Chlorella sp. using food processing waste as a cultivation medium</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2018</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cab27ae258b602c4735876cc46441276" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040848/Characterization_of_thermostable_cellulase_produced_by_Bacillus_strains_isolated_from_solid_waste_of_carrageenan"><img alt="Research paper thumbnail of Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040848/Characterization_of_thermostable_cellulase_produced_by_Bacillus_strains_isolated_from_solid_waste_of_carrageenan">Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacil...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. The optimum condition for cellulase production was obtained at pH and temperature of 8.0 and 50°C, respectively in a medium containing glucose as carbon source and 1.0% carboxymethyl cellulose (CMC) to stimulate the cellulase production. Most remarkably, the enzyme retained its relative activity over 50% after incubation at 50°C for 90 minutes. Substrate specificity suggested that the enzyme is an endoglucanase. The molecular mass of Bacillus licheniformis C55 crude cellulase was found about 18 kDa by SDS-PAGE analysis. This thermostable enzyme would facilitate development of more efficient and cost-effective forms of the process to convert lignocellulosic biomass into high-value products.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040848"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040848"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040848; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040848]").text(description); $(".js-view-count[data-work-id=119040848]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040848; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040848']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040848, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=119040848]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040848,"title":"Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan","translated_title":"","metadata":{"abstract":"Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. The optimum condition for cellulase production was obtained at pH and temperature of 8.0 and 50°C, respectively in a medium containing glucose as carbon source and 1.0% carboxymethyl cellulose (CMC) to stimulate the cellulase production. Most remarkably, the enzyme retained its relative activity over 50% after incubation at 50°C for 90 minutes. Substrate specificity suggested that the enzyme is an endoglucanase. The molecular mass of Bacillus licheniformis C55 crude cellulase was found about 18 kDa by SDS-PAGE analysis. This thermostable enzyme would facilitate development of more efficient and cost-effective forms of the process to convert lignocellulosic biomass into high-value products.","publisher":"IOP Publishing","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"IOP Conference Series: Earth and Environmental Science"},"translated_abstract":"Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. The optimum condition for cellulase production was obtained at pH and temperature of 8.0 and 50°C, respectively in a medium containing glucose as carbon source and 1.0% carboxymethyl cellulose (CMC) to stimulate the cellulase production. Most remarkably, the enzyme retained its relative activity over 50% after incubation at 50°C for 90 minutes. Substrate specificity suggested that the enzyme is an endoglucanase. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040705"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040705/Pengembangan_teknologi_emisi_akustik_untuk_deteksi_cepat_bakteri_patogen_Escherichia_coli_O157_H7_Salmonella_dan_Listeria_laporan_penelitian"><img alt="Research paper thumbnail of Pengembangan teknologi emisi akustik untuk deteksi cepat bakteri patogen (Escherichia coli O157:H7, Salmonella dan Listeria) : laporan penelitian" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040705/Pengembangan_teknologi_emisi_akustik_untuk_deteksi_cepat_bakteri_patogen_Escherichia_coli_O157_H7_Salmonella_dan_Listeria_laporan_penelitian">Pengembangan teknologi emisi akustik untuk deteksi cepat bakteri patogen (Escherichia coli O157:H7, Salmonella dan Listeria) : laporan penelitian</a></div><div class="wp-workCard_item"><span>Unknown Penerbit</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040705"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040705"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040705; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="113755815"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113755815/Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production"><img alt="Research paper thumbnail of Jobs Tears (Coix lacryma-jobi L.): the potentials of Indonesian under-utilized grains for herbal tea production" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113755815/Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production">Jobs Tears (Coix lacryma-jobi L.): the potentials of Indonesian under-utilized grains for herbal tea production</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains hig...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains highly nutritional and therapeutic values. Owing to its health beneficial compound, Jobs Tears can be considered as a nutraceutical. However, the utilization of this species is still limited due to a lack of awareness and attention from society. To promote utilization of Jobs Tears, by introducing its new acceptable and easy-to-eat products, this study aims to make new Jobs Tears-based herbal tea. Roasting is the critical stage in producing a high quality herbal tea. Roasting temperature and roasting duration treatment will affect the sensory qualities of the tea product. In this study, the different roasting temperatures (80, 100, 120°C) and roasting time (0.5, 1.0 and 1.5 hours) were combined to assess their effect on the organoleptic properties of Jobs Tears herbal tea. The organoleptic properties analysis showed that the best Jobs Tears herbal tea was achieved under a roasting temperatu...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113755815"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113755815"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113755815; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113755815]").text(description); $(".js-view-count[data-work-id=113755815]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113755815; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113755815']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 113755815, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113755815]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113755815,"title":"Jobs Tears (Coix lacryma-jobi L.): the potentials of Indonesian under-utilized grains for herbal tea production","translated_title":"","metadata":{"abstract":"Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains highly nutritional and therapeutic values. Owing to its health beneficial compound, Jobs Tears can be considered as a nutraceutical. However, the utilization of this species is still limited due to a lack of awareness and attention from society. To promote utilization of Jobs Tears, by introducing its new acceptable and easy-to-eat products, this study aims to make new Jobs Tears-based herbal tea. Roasting is the critical stage in producing a high quality herbal tea. Roasting temperature and roasting duration treatment will affect the sensory qualities of the tea product. In this study, the different roasting temperatures (80, 100, 120°C) and roasting time (0.5, 1.0 and 1.5 hours) were combined to assess their effect on the organoleptic properties of Jobs Tears herbal tea. The organoleptic properties analysis showed that the best Jobs Tears herbal tea was achieved under a roasting temperatu...","publisher":"IOP Publishing","publication_name":"IOP Conference Series: Earth and Environmental Science"},"translated_abstract":"Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains highly nutritional and therapeutic values. Owing to its health beneficial compound, Jobs Tears can be considered as a nutraceutical. However, the utilization of this species is still limited due to a lack of awareness and attention from society. To promote utilization of Jobs Tears, by introducing its new acceptable and easy-to-eat products, this study aims to make new Jobs Tears-based herbal tea. Roasting is the critical stage in producing a high quality herbal tea. Roasting temperature and roasting duration treatment will affect the sensory qualities of the tea product. In this study, the different roasting temperatures (80, 100, 120°C) and roasting time (0.5, 1.0 and 1.5 hours) were combined to assess their effect on the organoleptic properties of Jobs Tears herbal tea. The organoleptic properties analysis showed that the best Jobs Tears herbal tea was achieved under a roasting temperatu...","internal_url":"https://www.academia.edu/113755815/Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production","translated_internal_url":"","created_at":"2024-01-19T19:23:10.154-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[],"research_interests":[{"id":498,"name":"Physics","url":"https://www.academia.edu/Documents/in/Physics"},{"id":1040,"name":"Food Science","url":"https://www.academia.edu/Documents/in/Food_Science"},{"id":4086,"name":"Traditional Medicine","url":"https://www.academia.edu/Documents/in/Traditional_Medicine"},{"id":228471,"name":"Tears","url":"https://www.academia.edu/Documents/in/Tears"},{"id":1686654,"name":"Roasting","url":"https://www.academia.edu/Documents/in/Roasting"},{"id":2534010,"name":"organoleptic","url":"https://www.academia.edu/Documents/in/organoleptic"},{"id":2639492,"name":"IOP conference series-MSE","url":"https://www.academia.edu/Documents/in/IOP_conference_series-MSE"}],"urls":[{"id":38740671,"url":"https://iopscience.iop.org/article/10.1088/1755-1315/924/1/012042/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="113755814"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113755814/The_potency_of_bacteriophages_isolated_from_chicken_intestine_and_beef_tribe_to_control_biofilm_forming_bacteria_Bacillus_subtilis"><img alt="Research paper thumbnail of The potency of bacteriophages isolated from chicken intestine and beef tribe to control biofilm-forming bacteria, Bacillus subtilis" class="work-thumbnail" src="https://attachments.academia-assets.com/110636580/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113755814/The_potency_of_bacteriophages_isolated_from_chicken_intestine_and_beef_tribe_to_control_biofilm_forming_bacteria_Bacillus_subtilis">The potency of bacteriophages isolated from chicken intestine and beef tribe to control biofilm-forming bacteria, Bacillus subtilis</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, May 22, 2023</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dd2698d56395e8623d3bc3f825115c20" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110636580,"asset_id":113755814,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110636580/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113755814"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113755814"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113755814; 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To deal with the problem, an industry generally employs physical and chemical methods including sanitizers, disinfectants, and antimicrobials to remove biofilm. However, the use of these methods may bring about new problems, which are bacterial resistance in the biofilm and the risk for product contamination. New strategies to deal with bacterial biofilms are needed. Bacteriophages (phages), as a green alternative to chemical, have re-emerged as a promising approach to treat bacterial biofilm. In the present study, the potential of lytic phages which have antibiofilm activity on biofilm-forming bacteria (Bacillus subtilis), were isolated from chicken intestines and beef tripe obtained from Indonesian traditional markets using host cells obtained isolated from these samples. Phages isolation was conducted by using double layer agar technique. A lytic test of phages was administered on biofilm-forming bacteria. The difference of turbidity level between control (which were not infected by phages) and the test tubes containing host bacteria infected by phages was investigated. The infection time for the production of phages was determined based on the level of clarity of the media in the test tube with a longer lysate addition time. Three phages were isolated namely: ϕBS6, ϕBS8, and ϕUA7. It showed the ability to inhibit B. subtilis as biofilm-forming spoilage bacteria. The best inhibition results were obtained from ϕBS6. Infection with ϕBS6 in B. subtilis lead to 0.5 log cycle decreased in bacterial cells. This study showed that isolated phages might be used as a potential approach for handling the problem of biofilm formation by B. subtilis. Recent outbreaks of foodborne illness can be attributed to biofilms. Biofilm formation is an integral part of the microbial life cycle in nature. Foodborne pathogens form biofilms as a survival strategy in various unfavorable environments, which also become a frequent source of recurrent contamination and outbreaks of foodborne illness 1. Approximately 60 percent of foodborne illness outbreaks are caused by biofilms, according to food safety research 2. Biofilm is a form of bacterial adaptation that colonizes and attaches to the surface, covered by extracellular polymeric material 3. It becomes a major problem in the food, health, and marine industries 4. Eighty percent outbreaks due to pathogenic bacteria are contributed by biofilm-forming bacteria 5. The formation of biofilms by pathogenic bacteria, especially in the processing equipment, is a big challenge in the food industry. In the food industry-such as the brewing industry, dairy product processing, fresh product, and meat-the presence of biofilm within the production line will be a source of contamination for foodstuffs that pass through the production line 6-8. The formation of biofilms also generates a negative impact on non-food industry such as the oil drilling industry, paper production, health products/medicines 9,10. Biofilms bring about difficulties during the production process because they can reduce heat transfer, block tubes, filters, and it causes surface damage to equipment 11,12. The strategy that has so far been applied by industries to control the formation of biofilms is using chemicals, such as acid, oxidizing compounds (chlorine, H 2 O 2), and surfactants 13-15. However, the use of these chemicals","publication_date":{"day":22,"month":5,"year":2023,"errors":{}},"publication_name":"Scientific 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Cost-Effective Herbs to Counteract Obesity by Inhibiting PPAR-γ Gene Expression" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106742161/The_Use_of_Green_Coffee_and_Green_Tea_Extract_as_Cost_Effective_Herbs_to_Counteract_Obesity_by_Inhibiting_PPAR_%CE%B3_Gene_Expression">The Use of Green Coffee and Green Tea Extract as Cost-Effective Herbs to Counteract Obesity by Inhibiting PPAR-γ Gene Expression</a></div><div class="wp-workCard_item"><span>Advances in economics, business and management research</span><span>, 2023</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106742161"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106742161"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106742161; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=106742161]").text(description); $(".js-view-count[data-work-id=106742161]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x 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research"},"translated_abstract":null,"internal_url":"https://www.academia.edu/106742161/The_Use_of_Green_Coffee_and_Green_Tea_Extract_as_Cost_Effective_Herbs_to_Counteract_Obesity_by_Inhibiting_PPAR_%CE%B3_Gene_Expression","translated_internal_url":"","created_at":"2023-09-17T05:42:02.427-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_Use_of_Green_Coffee_and_Green_Tea_Extract_as_Cost_Effective_Herbs_to_Counteract_Obesity_by_Inhibiting_PPAR_γ_Gene_Expression","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna 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alt="Research paper thumbnail of Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp" class="work-thumbnail" src="https://attachments.academia-assets.com/105774746/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106742160/Leclercia_adecarboxylata_C12_The_Newly_Isolated_Cellulose_degrading_Bacteria_from_Indonesian_Coffee_Pulp">Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp</a></div><div class="wp-workCard_item"><span>HAYATI Journal of Biosciences</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Jav...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="90867376ab45bc9d7db9ffddb8654707" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":105774746,"asset_id":106742160,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/105774746/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106742160"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106742160"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106742160; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=106742160]").text(description); $(".js-view-count[data-work-id=106742160]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 106742160; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='106742160']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 106742160, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "90867376ab45bc9d7db9ffddb8654707" } } $('.js-work-strip[data-work-id=106742160]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":106742160,"title":"Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp","translated_title":"","metadata":{"abstract":"Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. 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The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="106742136"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/106742136/Bioethanol_Production_from_Sugarcane_Molasses_by_Fed_Batch_Fermentation_Systems_Using_Instant_Dry_Yeast"><img alt="Research paper thumbnail of Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast" class="work-thumbnail" src="https://attachments.academia-assets.com/105774738/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106742136/Bioethanol_Production_from_Sugarcane_Molasses_by_Fed_Batch_Fermentation_Systems_Using_Instant_Dry_Yeast">Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast</a></div><div class="wp-workCard_item"><span>Microbiology and Biotechnology Letters</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="199792cce75bdd29fe3920b1713d0074" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":105774738,"asset_id":106742136,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/105774738/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106742136"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106742136"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106742136; 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This study aimed to develop a potential process for bioethanol production by fed-batch fermentation using instant dry yeast. To obtain the highest cell growth, we studied the influence of the initial sugar concentrations and pH of sugarcane molasses in batch fermentation. The batch system employed three levels of sugar concentrations, viz. 10%, 15%, 20% (w/v), and two levels of pH, 5.0 and 5.5. The highest cell growth was achieved at 20% (w/v) and pH 5.5 of molasses. The fed-batch system was then performed using the best batch fermentation conditions, with a molasses concentration of 13% (w/v) which resulted in high ethanol concentration and fermentation efficiency of 15.96% and 89%, respectively.","publication_name":"Microbiology and Biotechnology 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alt="Research paper thumbnail of The Optimal Dose of the Green Tea and Coffee Extracts to Suppress the Expression of PPAR-γ and C/EBP-α on Differentiated 3T3-L1 Adipocytes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123412615/The_Optimal_Dose_of_the_Green_Tea_and_Coffee_Extracts_to_Suppress_the_Expression_of_PPAR_%CE%B3_and_C_EBP_%CE%B1_on_Differentiated_3T3_L1_Adipocytes">The Optimal Dose of the Green Tea and Coffee Extracts to Suppress the Expression of PPAR-γ and C/EBP-α on Differentiated 3T3-L1 Adipocytes</a></div><div class="wp-workCard_item"><span>Trends in Sciences</span><span>, Oct 30, 2023</span></div><div class="wp-workCard_item wp-workCard--actions"><span 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})(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "432e9a50ba8222bffc87410c6767b797" } } $('.js-work-strip[data-work-id=123412569]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123412569,"title":"Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production","translated_title":"","metadata":{"publisher":"Springer Science+Business Media","grobid_abstract":"The energy crisis triggers the use of energy sources that are renewable, such as biomass made from lignocellulosic materials, to produce various chemical compounds for food ingredients and biofuel. The efficient conversion of lignocellulosic biomass into products with added value involves the activity of microorganisms, such as yeasts. For the conversion, microorganisms must be able to use various sugars in lignocellulosic biomass, including pentose sugars, especially xylose. This study aims to isolate xylose-utilizing yeasts and analyze their fermentation activity to produce xylitol and ethanol, as well as their ability to grow in liquid hydrolysate produced from pretreated lignocellulosic biomass. Nineteen yeast isolates could grow on solid and liquid media using solely xylose as a carbon source. All isolates can grow in a xylose medium with incubation at 30 °C, 37 °C, 42 °C, and 45 °C. Six isolates, namely SLI (1), SL3, SL6, SL7, R5, and OPT4B, were chosen based on their considerable growth and high xylose consumption rate in a medium with 50 g/L xylose with incubation at 30 °C for 48 h. Four isolates tested, namely SLI (1), SL6, SL7, and R5, can produce xylitol in media containing xylose carbon sources. The concentration of xylitol produced was determined using highpressure liquid chromatography (HPLC), and the results ranged from 5.0 to 6.0 g/L. Five isolates tested, namely SLI (1), SL6, SL3, R5, and OPT4B, can produce ethanol. The ethanol content produced was determined using gas chromatography (GC), with concentrations ranging from 0.85 to 1.34 g/L. Three isolates, namely SL1(1), R5, and SL6, were able to produce xylitol and ethanol from xylose as carbon sources and were also able to grow on liquid hydrolyzate from pretreated oil palm trunk waste with the subcritical water method. The three isolates were further analyzed using the 18S rDNA sequence to identify the species and confirm their phylogenetic position. Identification based on DNA sequence analysis revealed that isolates SL1(1) and R5 were Pichia kudriavzevii, while isolate SL6 was Candida xylopsoci. The yeast strains isolated from this study could potentially be used for the bioconversion process of lignocellulosic biomass waste to produce value-added derivative products.","publication_date":{"day":12,"month":10,"year":2023,"errors":{}},"publication_name":"Bioresources and 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Science","url":"https://www.academia.edu/Documents/in/Food_Science"},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":56291,"name":"Ethanol Fuel","url":"https://www.academia.edu/Documents/in/Ethanol_Fuel"},{"id":71540,"name":"Xylitol","url":"https://www.academia.edu/Documents/in/Xylitol"},{"id":93647,"name":"Biofuel","url":"https://www.academia.edu/Documents/in/Biofuel"},{"id":131405,"name":"Hydrolysate","url":"https://www.academia.edu/Documents/in/Hydrolysate"},{"id":151659,"name":"Yeast","url":"https://www.academia.edu/Documents/in/Yeast"},{"id":269129,"name":"Fermentation","url":"https://www.academia.edu/Documents/in/Fermentation"},{"id":614751,"name":"Xylose","url":"https://www.academia.edu/Documents/in/Xylose"},{"id":1030794,"name":"Hydrolysis","url":"https://www.academia.edu/Documents/in/Hydrolysis"},{"id":3369889,"name":"Lignocellulosic Biomass","url":"https://www.academia.edu/Documents/in/Lignocellulosic_Biomass"}],"urls":[{"id":44369401,"url":"https://bioresourcesbioprocessing.springeropen.com/counter/pdf/10.1186/s40643-023-00691-y"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040855"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040855/In_Silico_Analysis_of_Active_Compounds_from_Herbal_Plants_Against_Penicillin_Binding_Protein_2a_PBP2a_of_Methicillin_Resistant_Staphylococcus_Aureus"><img alt="Research paper thumbnail of In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus" class="work-thumbnail" src="https://attachments.academia-assets.com/114516802/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040855/In_Silico_Analysis_of_Active_Compounds_from_Herbal_Plants_Against_Penicillin_Binding_Protein_2a_PBP2a_of_Methicillin_Resistant_Staphylococcus_Aureus">In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Herbal plants are frequently used for medication by people as they contain rich bioactive compoun...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. In sum, it suggested three compounds with the best potential ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="593da6a1b204b0a7aa0382c01397aea1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":114516802,"asset_id":119040855,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/114516802/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040855"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040855"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040855; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040855]").text(description); $(".js-view-count[data-work-id=119040855]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040855; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040855']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040855, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "593da6a1b204b0a7aa0382c01397aea1" } } $('.js-work-strip[data-work-id=119040855]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040855,"title":"In-Silico Analysis of Active Compounds from Herbal Plants Against Penicillin Binding Protein 2a (PBP2a) of Methicillin-Resistant Staphylococcus Aureus","translated_title":"","metadata":{"abstract":"Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. In sum, it suggested three compounds with the best potential ...","publisher":"Research Square Platform LLC"},"translated_abstract":"Herbal plants are frequently used for medication by people as they contain rich bioactive compounds. Turmeric (Curcuma longa) and bitter ginger (Zingiber zerumbet) are types of rhizome herbal plants with the highest amount of production in Indonesia. They contain bioactive compounds applicable for antibiotics against resistant bacteria, one of which is MRSA (Methicillin-resistant Staphylococcus aureus). This study aims to investigate the compound components in turmeric and bitter ginger, which might be anti-MRSA candidates against the PBP2a binding side by in-silico analysis. A total of 24 ligands of turmeric and bitter ginger are bound to the target protein, the PBP2a receptor. The binding results are followed with a test of biological activity, physicochemical properties, and toxicity of the herbal plant compounds. The study resulted in 12 ligands potentially being anti-MRSA with binding on the allosteric side of PBP2a. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040853"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040853/The_increasement_of_rice_straw_hydrolysis_using_blend_crude_cellulose_enzyme_from_Trichoderma_reesei_and_Aspergillus_niger"><img alt="Research paper thumbnail of The increasement of rice straw hydrolysis using blend crude cellulose enzyme from Trichoderma reesei and Aspergillus niger" class="work-thumbnail" src="https://attachments.academia-assets.com/114516771/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040853/The_increasement_of_rice_straw_hydrolysis_using_blend_crude_cellulose_enzyme_from_Trichoderma_reesei_and_Aspergillus_niger">The increasement of rice straw hydrolysis using blend crude cellulose enzyme from Trichoderma reesei and Aspergillus niger</a></div><div class="wp-workCard_item"><span>Research and reviews in biosciences</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. The use...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. The use of two crude enzyme separately only able to produce a low levels of glucose, but after the two crude enzyme being mixed, it is able to improve the yield of glucose on cellulose hydrolysis process by using rice straw as substrate which has undergone pretreatment by alkali microwave. The combination of crude enzyme from Trichoderma reesei and Aspergillus niger (2:1) has the FP-ase activity of 1,002 IU/ml, CMC-ase activity of 2.23 IU/ml and â-glucosidase activity of 0.168 IU/ml. The activity of the crude enzyme cellulose combination are able to generate sugar at 12.89 mg/ml in the process of hydrolysis for 72 hours.  2014 Trade Science Inc. - INDIA</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="9e8daa9de93d204a91a01bcdf5a54ef7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":114516771,"asset_id":119040853,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/114516771/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040853"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040853"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040853; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040853]").text(description); $(".js-view-count[data-work-id=119040853]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040853; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040853']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040853, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "9e8daa9de93d204a91a01bcdf5a54ef7" } } $('.js-work-strip[data-work-id=119040853]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040853,"title":"The increasement of rice straw hydrolysis using blend crude cellulose enzyme from Trichoderma reesei and Aspergillus niger","translated_title":"","metadata":{"abstract":"The crude enzyme from Trichoderma reesei and Aspergillus niger have different activities. 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Science","url":"https://www.academia.edu/Documents/in/Food_Science"},{"id":37959,"name":"Cellulose","url":"https://www.academia.edu/Documents/in/Cellulose"},{"id":43685,"name":"Cellulase","url":"https://www.academia.edu/Documents/in/Cellulase"},{"id":55171,"name":"Straw","url":"https://www.academia.edu/Documents/in/Straw"},{"id":139486,"name":"Sugar","url":"https://www.academia.edu/Documents/in/Sugar"},{"id":188234,"name":"Trichoderma","url":"https://www.academia.edu/Documents/in/Trichoderma"},{"id":215619,"name":"Aspergillus niger","url":"https://www.academia.edu/Documents/in/Aspergillus_niger"},{"id":1030794,"name":"Hydrolysis","url":"https://www.academia.edu/Documents/in/Hydrolysis"},{"id":1398164,"name":"Trichoderma Reesei","url":"https://www.academia.edu/Documents/in/Trichoderma_Reesei"}],"urls":[{"id":41928632,"url":"http://blog.ub.ac.id/jatmikoekotbp/files/2014/04/Jurnal-2-Jerami.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040852"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040852/The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F"><img alt="Research paper thumbnail of The effect of different substrates on chitinase activity from Bacillus sp. WS4F" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040852/The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F">The effect of different substrates on chitinase activity from Bacillus sp. WS4F</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2021</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for plant diseases caused by pathogenic fungi. Bacillus sp. WS4F has chitinase activity which can inhibit the growth of Ganoderma boninense, a fungus that attacks oil palm and causes basal stem rot (BSR). This study aims to investigate the effect of different substrates on the activity of the chitinase from Bacillus sp. WS4F. Two kinds of substrates i.e. chitin flakes and shrimp shells were used in this study. Enzyme activity of chitinase was analyzed after partial purification of enzyme was performed using ammonium sulfate precipitation followed by dialysis. The highest activity of chitinase was achieved by the substrate using shrimp shells. The ammonium sulfate precipitation (60-80% saturation) 0.0949 U/mL for activity enzyme and 0.2639 mg/mL for protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040852"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040852"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040852; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040852]").text(description); $(".js-view-count[data-work-id=119040852]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040852; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040852']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040852, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=119040852]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040852,"title":"The effect of different substrates on chitinase activity from Bacillus sp. 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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.","publisher":"IOP Publishing","publication_date":{"day":null,"month":null,"year":2021,"errors":{}},"publication_name":"IOP Conference Series: Earth and Environmental Science"},"translated_abstract":"One of the roles of chitinase is as an antifungal which is widely used as a biocontrol agent for plant diseases caused by pathogenic fungi. Bacillus sp. WS4F has chitinase activity which can inhibit the growth of Ganoderma boninense, a fungus that attacks oil palm and causes basal stem rot (BSR). This study aims to investigate the effect of different substrates on the activity of the chitinase from Bacillus sp. WS4F. Two kinds of substrates i.e. chitin flakes and shrimp shells were used in this study. Enzyme activity of chitinase was analyzed after partial purification of enzyme was performed using ammonium sulfate precipitation followed by dialysis. The highest activity of chitinase was achieved by the substrate using shrimp shells. The ammonium sulfate precipitation (60-80% saturation) 0.0949 U/mL for activity enzyme and 0.2639 mg/mL for protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 64.389 kDa.","internal_url":"https://www.academia.edu/119040852/The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F","translated_internal_url":"","created_at":"2024-05-13T19:48:56.413-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_effect_of_different_substrates_on_chitinase_activity_from_Bacillus_sp_WS4F","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[],"research_interests":[{"id":498,"name":"Physics","url":"https://www.academia.edu/Documents/in/Physics"},{"id":529713,"name":"Chitinase","url":"https://www.academia.edu/Documents/in/Chitinase"},{"id":954401,"name":"Enzyme Assay","url":"https://www.academia.edu/Documents/in/Enzyme_Assay"},{"id":1169238,"name":"Chitin","url":"https://www.academia.edu/Documents/in/Chitin"},{"id":1363689,"name":"Ammonium Sulfate","url":"https://www.academia.edu/Documents/in/Ammonium_Sulfate"},{"id":2639492,"name":"IOP conference series-MSE","url":"https://www.academia.edu/Documents/in/IOP_conference_series-MSE"}],"urls":[{"id":41928631,"url":"https://iopscience.iop.org/article/10.1088/1755-1315/924/1/012034/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040851"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/119040851/Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka"><img alt="Research paper thumbnail of Produksi Protein Sel Tunggal Dari Biomasa Mikroorganisme : Kajian Pustaka" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/119040851/Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka">Produksi Protein Sel Tunggal Dari Biomasa Mikroorganisme : Kajian Pustaka</a></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040851"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040851"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040851; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040851]").text(description); $(".js-view-count[data-work-id=119040851]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040851; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040851']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040851, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=119040851]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040851,"title":"Produksi Protein Sel Tunggal Dari Biomasa Mikroorganisme : Kajian Pustaka","translated_title":"","metadata":{"publication_date":{"day":null,"month":null,"year":2015,"errors":{}}},"translated_abstract":null,"internal_url":"https://www.academia.edu/119040851/Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka","translated_internal_url":"","created_at":"2024-05-13T19:48:56.272-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Produksi_Protein_Sel_Tunggal_Dari_Biomasa_Mikroorganisme_Kajian_Pustaka","translated_slug":"","page_count":null,"language":"id","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040850"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040850/Isolation_screening_and_identification_of_potential_cellulolytic_and_xylanolytic_mold_from_oil_palm_waste"><img alt="Research paper thumbnail of Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste" class="work-thumbnail" src="https://attachments.academia-assets.com/114516801/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040850/Isolation_screening_and_identification_of_potential_cellulolytic_and_xylanolytic_mold_from_oil_palm_waste">Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The objective of this research was to isolate, screen, and identify the cellulase and xylanase-pr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The objective of this research was to isolate, screen, and identify the cellulase and xylanase-producing mold from oil palm waste. There are thirty-two isolates from oil palm waste which are able to degrade and grow on media containing cellulose or xylan as a sole carbon source. Screening to determine cellulolytic and xylanolytic activity was performed by paper disc diffusion method using Congo Red as an indicator. All the thirty-two mold isolates showed cellulolytic and xylanolytic activity with relative enzyme activity (ratio of hydrolysed zone diameter and diameter of colony growth) ranging from 1.04 to 1.62. Based on the macro- and micromorphology characteristics, these isolates were identified as genus Trichoderma, Aspergillus, Rhizopus, Tallaromyces and Penicillium, with the number isolates in each genus was 22, 4, 3, 2, and 1 respectively. The highest cellulolytic and xylanolytic activity was achieved from the isolate namely OPT1(4) which was identified as Talaromyces pinophi...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="260fa1b1d1f91df1b097153f2680ce62" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":114516801,"asset_id":119040850,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/114516801/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3Myw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040850"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040850"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040850; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040850]").text(description); $(".js-view-count[data-work-id=119040850]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040850; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040850']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040850, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "260fa1b1d1f91df1b097153f2680ce62" } } $('.js-work-strip[data-work-id=119040850]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040850,"title":"Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste","translated_title":"","metadata":{"abstract":"The objective of this research was to isolate, screen, and identify the cellulase and xylanase-producing mold from oil palm waste. 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cultivation medium" class="work-thumbnail" src="https://attachments.academia-assets.com/114516767/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040849/Single_cell_protein_production_of_Chlorella_sp_using_food_processing_waste_as_a_cultivation_medium">Single cell protein production of Chlorella sp. using food processing waste as a cultivation medium</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span><span>, 2018</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="cab27ae258b602c4735876cc46441276" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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Conference Series: Earth and Environmental Science</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacil...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. The optimum condition for cellulase production was obtained at pH and temperature of 8.0 and 50°C, respectively in a medium containing glucose as carbon source and 1.0% carboxymethyl cellulose (CMC) to stimulate the cellulase production. Most remarkably, the enzyme retained its relative activity over 50% after incubation at 50°C for 90 minutes. Substrate specificity suggested that the enzyme is an endoglucanase. The molecular mass of Bacillus licheniformis C55 crude cellulase was found about 18 kDa by SDS-PAGE analysis. This thermostable enzyme would facilitate development of more efficient and cost-effective forms of the process to convert lignocellulosic biomass into high-value products.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040848"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040848"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040848; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=119040848]").text(description); $(".js-view-count[data-work-id=119040848]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 119040848; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='119040848']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 119040848, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=119040848]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":119040848,"title":"Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan","translated_title":"","metadata":{"abstract":"Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="119040705"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/119040705/Pengembangan_teknologi_emisi_akustik_untuk_deteksi_cepat_bakteri_patogen_Escherichia_coli_O157_H7_Salmonella_dan_Listeria_laporan_penelitian"><img alt="Research paper thumbnail of Pengembangan teknologi emisi akustik untuk deteksi cepat bakteri patogen (Escherichia coli O157:H7, Salmonella dan Listeria) : laporan penelitian" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/119040705/Pengembangan_teknologi_emisi_akustik_untuk_deteksi_cepat_bakteri_patogen_Escherichia_coli_O157_H7_Salmonella_dan_Listeria_laporan_penelitian">Pengembangan teknologi emisi akustik untuk deteksi cepat bakteri patogen (Escherichia coli O157:H7, Salmonella dan Listeria) : laporan penelitian</a></div><div class="wp-workCard_item"><span>Unknown Penerbit</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="119040705"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="119040705"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 119040705; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="113755815"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113755815/Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production"><img alt="Research paper thumbnail of Jobs Tears (Coix lacryma-jobi L.): the potentials of Indonesian under-utilized grains for herbal tea production" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113755815/Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production">Jobs Tears (Coix lacryma-jobi L.): the potentials of Indonesian under-utilized grains for herbal tea production</a></div><div class="wp-workCard_item"><span>IOP Conference Series: Earth and Environmental Science</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains hig...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains highly nutritional and therapeutic values. Owing to its health beneficial compound, Jobs Tears can be considered as a nutraceutical. However, the utilization of this species is still limited due to a lack of awareness and attention from society. To promote utilization of Jobs Tears, by introducing its new acceptable and easy-to-eat products, this study aims to make new Jobs Tears-based herbal tea. Roasting is the critical stage in producing a high quality herbal tea. Roasting temperature and roasting duration treatment will affect the sensory qualities of the tea product. In this study, the different roasting temperatures (80, 100, 120°C) and roasting time (0.5, 1.0 and 1.5 hours) were combined to assess their effect on the organoleptic properties of Jobs Tears herbal tea. The organoleptic properties analysis showed that the best Jobs Tears herbal tea was achieved under a roasting temperatu...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113755815"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113755815"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113755815; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113755815]").text(description); $(".js-view-count[data-work-id=113755815]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113755815; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113755815']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 113755815, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=113755815]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113755815,"title":"Jobs Tears (Coix lacryma-jobi L.): the potentials of Indonesian under-utilized grains for herbal tea production","translated_title":"","metadata":{"abstract":"Jobs Tears (Coix lacryma-jobi L.) is one of the potential local food resources which contains highly nutritional and therapeutic values. 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In this study, the different roasting temperatures (80, 100, 120°C) and roasting time (0.5, 1.0 and 1.5 hours) were combined to assess their effect on the organoleptic properties of Jobs Tears herbal tea. The organoleptic properties analysis showed that the best Jobs Tears herbal tea was achieved under a roasting temperatu...","internal_url":"https://www.academia.edu/113755815/Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production","translated_internal_url":"","created_at":"2024-01-19T19:23:10.154-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Jobs_Tears_Coix_lacryma_jobi_L_the_potentials_of_Indonesian_under_utilized_grains_for_herbal_tea_production","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[],"research_interests":[{"id":498,"name":"Physics","url":"https://www.academia.edu/Documents/in/Physics"},{"id":1040,"name":"Food Science","url":"https://www.academia.edu/Documents/in/Food_Science"},{"id":4086,"name":"Traditional Medicine","url":"https://www.academia.edu/Documents/in/Traditional_Medicine"},{"id":228471,"name":"Tears","url":"https://www.academia.edu/Documents/in/Tears"},{"id":1686654,"name":"Roasting","url":"https://www.academia.edu/Documents/in/Roasting"},{"id":2534010,"name":"organoleptic","url":"https://www.academia.edu/Documents/in/organoleptic"},{"id":2639492,"name":"IOP conference series-MSE","url":"https://www.academia.edu/Documents/in/IOP_conference_series-MSE"}],"urls":[{"id":38740671,"url":"https://iopscience.iop.org/article/10.1088/1755-1315/924/1/012042/pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="113755814"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/113755814/The_potency_of_bacteriophages_isolated_from_chicken_intestine_and_beef_tribe_to_control_biofilm_forming_bacteria_Bacillus_subtilis"><img alt="Research paper thumbnail of The potency of bacteriophages isolated from chicken intestine and beef tribe to control biofilm-forming bacteria, Bacillus subtilis" class="work-thumbnail" src="https://attachments.academia-assets.com/110636580/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/113755814/The_potency_of_bacteriophages_isolated_from_chicken_intestine_and_beef_tribe_to_control_biofilm_forming_bacteria_Bacillus_subtilis">The potency of bacteriophages isolated from chicken intestine and beef tribe to control biofilm-forming bacteria, Bacillus subtilis</a></div><div class="wp-workCard_item"><span>Scientific Reports</span><span>, May 22, 2023</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="dd2698d56395e8623d3bc3f825115c20" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110636580,"asset_id":113755814,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110636580/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="113755814"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="113755814"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 113755814; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=113755814]").text(description); $(".js-view-count[data-work-id=113755814]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 113755814; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='113755814']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 113755814, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "dd2698d56395e8623d3bc3f825115c20" } } $('.js-work-strip[data-work-id=113755814]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":113755814,"title":"The potency of bacteriophages isolated from chicken intestine and beef tribe to control biofilm-forming bacteria, Bacillus subtilis","translated_title":"","metadata":{"publisher":"Nature Portfolio","grobid_abstract":"Biofilm becomes one of the crucial food safety problems in the food industry as the formation of biofilm can be a source of contamination. To deal with the problem, an industry generally employs physical and chemical methods including sanitizers, disinfectants, and antimicrobials to remove biofilm. However, the use of these methods may bring about new problems, which are bacterial resistance in the biofilm and the risk for product contamination. New strategies to deal with bacterial biofilms are needed. Bacteriophages (phages), as a green alternative to chemical, have re-emerged as a promising approach to treat bacterial biofilm. In the present study, the potential of lytic phages which have antibiofilm activity on biofilm-forming bacteria (Bacillus subtilis), were isolated from chicken intestines and beef tripe obtained from Indonesian traditional markets using host cells obtained isolated from these samples. Phages isolation was conducted by using double layer agar technique. A lytic test of phages was administered on biofilm-forming bacteria. The difference of turbidity level between control (which were not infected by phages) and the test tubes containing host bacteria infected by phages was investigated. The infection time for the production of phages was determined based on the level of clarity of the media in the test tube with a longer lysate addition time. Three phages were isolated namely: ϕBS6, ϕBS8, and ϕUA7. It showed the ability to inhibit B. subtilis as biofilm-forming spoilage bacteria. The best inhibition results were obtained from ϕBS6. Infection with ϕBS6 in B. subtilis lead to 0.5 log cycle decreased in bacterial cells. This study showed that isolated phages might be used as a potential approach for handling the problem of biofilm formation by B. subtilis. Recent outbreaks of foodborne illness can be attributed to biofilms. Biofilm formation is an integral part of the microbial life cycle in nature. Foodborne pathogens form biofilms as a survival strategy in various unfavorable environments, which also become a frequent source of recurrent contamination and outbreaks of foodborne illness 1. Approximately 60 percent of foodborne illness outbreaks are caused by biofilms, according to food safety research 2. Biofilm is a form of bacterial adaptation that colonizes and attaches to the surface, covered by extracellular polymeric material 3. It becomes a major problem in the food, health, and marine industries 4. Eighty percent outbreaks due to pathogenic bacteria are contributed by biofilm-forming bacteria 5. The formation of biofilms by pathogenic bacteria, especially in the processing equipment, is a big challenge in the food industry. In the food industry-such as the brewing industry, dairy product processing, fresh product, and meat-the presence of biofilm within the production line will be a source of contamination for foodstuffs that pass through the production line 6-8. The formation of biofilms also generates a negative impact on non-food industry such as the oil drilling industry, paper production, health products/medicines 9,10. Biofilms bring about difficulties during the production process because they can reduce heat transfer, block tubes, filters, and it causes surface damage to equipment 11,12. The strategy that has so far been applied by industries to control the formation of biofilms is using chemicals, such as acid, oxidizing compounds (chlorine, H 2 O 2), and surfactants 13-15. However, the use of these chemicals","publication_date":{"day":22,"month":5,"year":2023,"errors":{}},"publication_name":"Scientific 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Cost-Effective Herbs to Counteract Obesity by Inhibiting PPAR-γ Gene Expression" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106742161/The_Use_of_Green_Coffee_and_Green_Tea_Extract_as_Cost_Effective_Herbs_to_Counteract_Obesity_by_Inhibiting_PPAR_%CE%B3_Gene_Expression">The Use of Green Coffee and Green Tea Extract as Cost-Effective Herbs to Counteract Obesity by Inhibiting PPAR-γ Gene Expression</a></div><div class="wp-workCard_item"><span>Advances in economics, business and management research</span><span>, 2023</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106742161"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106742161"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106742161; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=106742161]").text(description); $(".js-view-count[data-work-id=106742161]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x 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research"},"translated_abstract":null,"internal_url":"https://www.academia.edu/106742161/The_Use_of_Green_Coffee_and_Green_Tea_Extract_as_Cost_Effective_Herbs_to_Counteract_Obesity_by_Inhibiting_PPAR_%CE%B3_Gene_Expression","translated_internal_url":"","created_at":"2023-09-17T05:42:02.427-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"The_Use_of_Green_Coffee_and_Green_Tea_Extract_as_Cost_Effective_Herbs_to_Counteract_Obesity_by_Inhibiting_PPAR_γ_Gene_Expression","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna 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alt="Research paper thumbnail of Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp" class="work-thumbnail" src="https://attachments.academia-assets.com/105774746/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106742160/Leclercia_adecarboxylata_C12_The_Newly_Isolated_Cellulose_degrading_Bacteria_from_Indonesian_Coffee_Pulp">Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp</a></div><div class="wp-workCard_item"><span>HAYATI Journal of Biosciences</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Jav...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="90867376ab45bc9d7db9ffddb8654707" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":105774746,"asset_id":106742160,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/105774746/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106742160"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106742160"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106742160; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=106742160]").text(description); $(".js-view-count[data-work-id=106742160]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 106742160; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='106742160']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 106742160, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "90867376ab45bc9d7db9ffddb8654707" } } $('.js-work-strip[data-work-id=106742160]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":106742160,"title":"Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp","translated_title":"","metadata":{"abstract":"Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. 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The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA...","publisher":"Bogor Agricultural University","publication_name":"HAYATI Journal of Biosciences"},"translated_abstract":"Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="106742136"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/106742136/Bioethanol_Production_from_Sugarcane_Molasses_by_Fed_Batch_Fermentation_Systems_Using_Instant_Dry_Yeast"><img alt="Research paper thumbnail of Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast" class="work-thumbnail" src="https://attachments.academia-assets.com/105774738/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106742136/Bioethanol_Production_from_Sugarcane_Molasses_by_Fed_Batch_Fermentation_Systems_Using_Instant_Dry_Yeast">Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast</a></div><div class="wp-workCard_item"><span>Microbiology and Biotechnology Letters</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="199792cce75bdd29fe3920b1713d0074" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":105774738,"asset_id":106742136,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/105774738/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106742136"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106742136"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106742136; 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This study aimed to develop a potential process for bioethanol production by fed-batch fermentation using instant dry yeast. To obtain the highest cell growth, we studied the influence of the initial sugar concentrations and pH of sugarcane molasses in batch fermentation. The batch system employed three levels of sugar concentrations, viz. 10%, 15%, 20% (w/v), and two levels of pH, 5.0 and 5.5. The highest cell growth was achieved at 20% (w/v) and pH 5.5 of molasses. The fed-batch system was then performed using the best batch fermentation conditions, with a molasses concentration of 13% (w/v) which resulted in high ethanol concentration and fermentation efficiency of 15.96% and 89%, respectively.","publication_name":"Microbiology and Biotechnology Letters","grobid_abstract_attachment_id":105774738},"translated_abstract":null,"internal_url":"https://www.academia.edu/106742136/Bioethanol_Production_from_Sugarcane_Molasses_by_Fed_Batch_Fermentation_Systems_Using_Instant_Dry_Yeast","translated_internal_url":"","created_at":"2023-09-17T05:41:11.534-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":98296350,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":105774738,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/105774738/thumbnails/1.jpg","file_name":"mbl-51-2-184.pdf","download_url":"https://www.academia.edu/attachments/105774738/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Bioethanol_Production_from_Sugarcane_Mol.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/105774738/mbl-51-2-184-libre.pdf?1694956679=\u0026response-content-disposition=attachment%3B+filename%3DBioethanol_Production_from_Sugarcane_Mol.pdf\u0026Expires=1732534874\u0026Signature=U3WSBezk8NI6~40FC~r-2fB243bv726HVtNIGzkQFi3V6Npt4RVqKpka79ZQpY17lsJsT7yMmoydxVfvKLfhcQ43ijrXGkQxB1og9~sOEXBDy-KwoT1MSnqezrc07occ8fqUORY8j7n0tiLyRXz6u9oj40xsCSU2mbCTpTnRula75BMv4P5nMBtDsrk5vmqzfiU361nBZq4MGKICt-8CxQ0T8ZIwpXaOk9z~kt3uf9~fezgN4PlEqDhD4pOsVX5WuMyJWAuQkkiLPoO00hSAu84g~UzOfTy3Xmnjpc5Oq4VRaP-tXmBWAbwE4Wk9KKOsAnmSB6cmFkNc06T2jAR0Jg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Bioethanol_Production_from_Sugarcane_Molasses_by_Fed_Batch_Fermentation_Systems_Using_Instant_Dry_Yeast","translated_slug":"","page_count":7,"language":"en","content_type":"Work","owner":{"id":98296350,"first_name":"Dr.","middle_initials":null,"last_name":"Agustin Krisna W.,STP,M.Si","page_name":"DrAgustinKrisnaWSTPMSi","domain_name":"wordperss","created_at":"2018-11-29T10:40:45.197-08:00","display_name":"Dr. Agustin Krisna W.,STP,M.Si","url":"https://wordperss.academia.edu/DrAgustinKrisnaWSTPMSi"},"attachments":[{"id":105774738,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/105774738/thumbnails/1.jpg","file_name":"mbl-51-2-184.pdf","download_url":"https://www.academia.edu/attachments/105774738/download_file?st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&st=MTczMjUzMTI3NCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Bioethanol_Production_from_Sugarcane_Mol.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/105774738/mbl-51-2-184-libre.pdf?1694956679=\u0026response-content-disposition=attachment%3B+filename%3DBioethanol_Production_from_Sugarcane_Mol.pdf\u0026Expires=1732534874\u0026Signature=U3WSBezk8NI6~40FC~r-2fB243bv726HVtNIGzkQFi3V6Npt4RVqKpka79ZQpY17lsJsT7yMmoydxVfvKLfhcQ43ijrXGkQxB1og9~sOEXBDy-KwoT1MSnqezrc07occ8fqUORY8j7n0tiLyRXz6u9oj40xsCSU2mbCTpTnRula75BMv4P5nMBtDsrk5vmqzfiU361nBZq4MGKICt-8CxQ0T8ZIwpXaOk9z~kt3uf9~fezgN4PlEqDhD4pOsVX5WuMyJWAuQkkiLPoO00hSAu84g~UzOfTy3Xmnjpc5Oq4VRaP-tXmBWAbwE4Wk9KKOsAnmSB6cmFkNc06T2jAR0Jg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":105774737,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/105774737/thumbnails/1.jpg","file_name":"mbl-51-2-184.pdf","download_url":"https://www.academia.edu/attachments/105774737/download_file","bulk_download_file_name":"Bioethanol_Production_from_Sugarcane_Mol.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/105774737/mbl-51-2-184-libre.pdf?1694956683=\u0026response-content-disposition=attachment%3B+filename%3DBioethanol_Production_from_Sugarcane_Mol.pdf\u0026Expires=1732534874\u0026Signature=eYlEPh~3pyh2Jq6s3OmgMBgWlc-fFGDJxDycXuG9PfOstCzFCtOlE-5kk1HDlHxNv-n5V63JTJSA-rNzfC5BzSaKjMW5TCyLKw2NOzB-~fQgOsmY~mp0Rtl-~b8KsWu0ERHotu4GtiV93wdzheoP1J6cbUtBuOz7jDF0-gmFBKmrUa~euLj9u-md81uqh1jKEyZdx~m5GMeqr7-0gRHe5tFzmAm-f52l-dmrBPoYA8KSG10voNMMzhPxOgg6NiP9GjFKIepDNqK0DJXzcDwQAC-JTxl9yz4Qoh6NesHLdTrDprkfi7Y1ZtbRRj1jJNpiWC3texdMM1RPQBjiOuhWjA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[],"urls":[{"id":33956106,"url":"http://pdf.medrang.co.kr/MBL/2023/051/mbl-51-2-184.pdf"}]}, dispatcherData: dispatcherData }); 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