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TLC) were assessed as alternatives to the analysis of purine derivatives (allantoin, uric acid, xanthine and hypoxanthine) using classical spectrometry method, still largely used in SouthEastern Europe. With HPLC technique, very good calibration curves were obtained for all four purine derivatives (r\u003e0.999). Also, detection limits were satisfactory: ≥ 7.5 µg/mL for allantoin, ≥ 2.7 µg/mL for uric acid, ≥ 5.5 µg/mL for xanthine and ≥ 10.02 µg/mL for hypoxanthine. The analysis of urine samples taken from dairy cows gave results which were in the range of literature data for allantoin and uric acid, but not for hypoxanthine (whose concentration was much higher than values reported in literature). TLC technique allowed good separation of standards and elaboration of good calibration curves (r\u003e0.984). Detection limits were too high, if the whole range of values reported in literature for the concentrations purine derivatives in ruminants' urine is taken into account. Moreover, on urine samples the spots of creatinie overlapped the spots of uric acids, impairing the quantitative estimation of purine derivatives. It is concluded that HPLC technique can be used to assess the concentration of allantoin and uric acid in the urine of cows. The TLC technique can be used qualitatively, further studies on samples preparation and procedure being needed in order to use it for quantitative determinations.","publication_date":"2009,,","publication_name":"Archiva Zootechnica","grobid_abstract_attachment_id":"88734355"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Determination of purine derivatives in bovine urine using rapid chromatographic techniques","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [42735394]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":88734355,"attachmentType":"pdf"}"><img alt="First page of “Determination of purine derivatives in bovine urine using rapid chromatographic techniques”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/88734355/mini_magick20220718-32682-1wqdv5s.png?1658165608" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Determination of purine derivatives in bovine urine using rapid chromatographic techniques</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="42735394" href="https://independent.academia.edu/VComan1"><img alt="Profile image of V. Coman" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />V. Coman</a></div><p class="ds-work-card--detail ds2-5-body-sm">2009, Archiva Zootechnica</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":88734355,"attachmentType":"pdf","workUrl":"https://www.academia.edu/83367834/Determination_of_purine_derivatives_in_bovine_urine_using_rapid_chromatographic_techniques"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":88734355,"attachmentType":"pdf","workUrl":"https://www.academia.edu/83367834/Determination_of_purine_derivatives_in_bovine_urine_using_rapid_chromatographic_techniques"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="88734355" data-landing_url="https://www.academia.edu/83367834/Determination_of_purine_derivatives_in_bovine_urine_using_rapid_chromatographic_techniques" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="54661655" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/54661655/Simultaneous_determination_of_purine_derivatives_in_urine_by_high_performance_liquid_chromatography">Simultaneous determination of purine derivatives in urine by high-performance liquid chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="47058132" href="https://independent.academia.edu/MCzauderna">M. Czauderna</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Animal and Feed Sciences</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Simultaneous determination of purine derivatives in urine by high-performance liquid chromatography","attachmentId":70918834,"attachmentType":"pdf","work_url":"https://www.academia.edu/54661655/Simultaneous_determination_of_purine_derivatives_in_urine_by_high_performance_liquid_chromatography","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/54661655/Simultaneous_determination_of_purine_derivatives_in_urine_by_high_performance_liquid_chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="24408052" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/24408052/Quantification_of_allantoin_uric_acid_xanthine_and_hypoxanthine_in_ovine_urine_by_high_performance_liquid_chromatography_and_photodiode_array_detection">Quantification of allantoin, uric acid, xanthine and hypoxanthine in ovine urine by high-performance liquid chromatography and photodiode array detection</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="47058132" href="https://independent.academia.edu/MCzauderna">M. Czauderna</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of chromatography. B, Biomedical sciences and applications, 2000</p><p class="ds-related-work--abstract ds2-5-body-sm">A HPLC method for the determination of allantoin, uric acid, hypoxanthine and xanthine (purine metabolites) in ovine urine without the disadvantages inherent in derivatization is described. After dilution 1:6 with water, urine samples were injected onto the column. Separation and quantification of purine metabolites was achieved using two Nova-Pak C18 columns (4 microm, 250x4.6 mm, Waters). A binary gradient program and UV detection were used for purine metabolites analysis. Clear resolution of purine metabolites was obtained in less than 15 min. Allantoin, uric acid, hypoxanthine and xanthine in the effluent were monitored at 225, 284, 250 and 267 nm, respectively. The average recoveries of purine metabolite standards added to urine samples were satisfactory (100.2-102.9%). The low coefficients of variation (0.29-0.73%) as well as the low detection (0.16-0.70 nmol) and quantification (0.52-2.32 nmol) limits indicate satisfactory precision, reproducibility and sensitivity of the pro...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Quantification of allantoin, uric acid, xanthine and hypoxanthine in ovine urine by high-performance liquid chromatography and photodiode array detection","attachmentId":44739240,"attachmentType":"pdf","work_url":"https://www.academia.edu/24408052/Quantification_of_allantoin_uric_acid_xanthine_and_hypoxanthine_in_ovine_urine_by_high_performance_liquid_chromatography_and_photodiode_array_detection","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/24408052/Quantification_of_allantoin_uric_acid_xanthine_and_hypoxanthine_in_ovine_urine_by_high_performance_liquid_chromatography_and_photodiode_array_detection"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="95392411" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/95392411/Improved_HPLC_method_for_the_simultaneous_determination_of_allantoin_uric_acid_and_creatinine_in_cattle_urine">Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="228748017" href="https://independent.academia.edu/YongchuanGu">Yongchuan Gu</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Chromatography B, 2006</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine","attachmentId":97588067,"attachmentType":"pdf","work_url":"https://www.academia.edu/95392411/Improved_HPLC_method_for_the_simultaneous_determination_of_allantoin_uric_acid_and_creatinine_in_cattle_urine","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/95392411/Improved_HPLC_method_for_the_simultaneous_determination_of_allantoin_uric_acid_and_creatinine_in_cattle_urine"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="103429673" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/103429673/Simultaneous_quantifications_of_four_purine_derivatives_biomarkers_in_cow_milk_by_SPE_HPLC_DAD">Simultaneous quantifications of four purine derivatives biomarkers in cow milk by SPE HPLC-DAD</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="273933501" href="https://independent.academia.edu/mvlassa">mihaela vlassa</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Czech Journal of Food Sciences, 2021</p><p class="ds-related-work--abstract ds2-5-body-sm">In this study, simultaneous quantification of allantoin, uric acid, xanthine, and hypoxanthine in cow milk by solid phase extraction (SPE) and high performance liquid chromatography-diode array detection (HPLC-DAD) method was perform. Five different SPE cartridges were tested in order to evaluate the isolation of purine derivatives (PD) from cow milk. Chromatography was carried out on ODS-2 Hypersil column and 0.05 M (NH4)2HPO4 buffer solution (pH = 7.76) as mobile phase. The HPLC-DAD validated method showed a linearity with regression coefficients higher than 0.999 and the limits of detection and quantification with values in the range 0.09–0.74 µg mL–1 and 0.27–2.24 µg mL–1, respectively. The method showed good precision with a relative standard deviation (RSD) below 4.48%, while the accuracy ranged from 95.34 to 104.47% for all analytes. The best recovery degree of PD by SPE were obtained on Strata SCX cartridge for xanthine (87.79%) and hypoxanthine (89.02%); on Strata NH2 for a...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Simultaneous quantifications of four purine derivatives biomarkers in cow milk by SPE HPLC-DAD","attachmentId":103438928,"attachmentType":"pdf","work_url":"https://www.academia.edu/103429673/Simultaneous_quantifications_of_four_purine_derivatives_biomarkers_in_cow_milk_by_SPE_HPLC_DAD","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/103429673/Simultaneous_quantifications_of_four_purine_derivatives_biomarkers_in_cow_milk_by_SPE_HPLC_DAD"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="125126468" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/125126468/Urinary_excretion_of_purine_derivatives_inBos_indicus_Bos_tauruscrossbred_cattle">Urinary excretion of purine derivatives inBos indicus×Bos tauruscrossbred cattle</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="303349941" href="https://independent.academia.edu/BelenguerAlvaro">Alvaro Belenguer</a></div><p class="ds-related-work--metadata ds2-5-body-xs">British Journal of Nutrition, 2005</p><p class="ds-related-work--abstract ds2-5-body-sm">Four experiments were performed to study the kinetics of purine metabolism and urinary excretion in Zebu crossbred cattle. Fasting excretion was established in Expt 1, using eighteen maleBos indicus×Bos tauruscrossbred cattle (261 (se 9·1) kg body weight), six of each of the following genotypes: 5/8Bos indicus, 1/2Bos indicusand 3/8Bos indicus. No significant differences were observed among genotypes in fasting purine derivative excretion (277·3 (se 35·43) μmol/metabolic body weight). In a second experiment we measured the xanthine oxidase activity, which was higher in liver than in duodenal mucosa (0·64 and 0·06 (se 0·12) units/g wet tissue per min respectively;P&gt;0·05) being in plasma 0·60 (se 0·36) units/l per min. The kinetics of uric acid were measured by intravenous pulse dose of [1,3-15N]uric acid (Expt 3). The cumulative recovery of the isotope in urine was 82 (se 6·69) %, and uric acid plasma removal, pool size and mean retention time were 0·284 (se 0·051) per h, 5·45 (se...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives inBos indicus×Bos tauruscrossbred cattle","attachmentId":119225117,"attachmentType":"pdf","work_url":"https://www.academia.edu/125126468/Urinary_excretion_of_purine_derivatives_inBos_indicus_Bos_tauruscrossbred_cattle","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/125126468/Urinary_excretion_of_purine_derivatives_inBos_indicus_Bos_tauruscrossbred_cattle"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="59683647" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/59683647/Endogenous_fraction_and_urinary_recovery_of_purine_derivatives_obtained_by_different_methods_in_Nellore_cattle">Endogenous fraction and urinary recovery of purine derivatives obtained by different methods in Nellore cattle</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="67255054" href="https://independent.academia.edu/Anal%C3%ADviaBarbosa">Analívia Barbosa</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Animal Science, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Endogenous fraction and urinary recovery of purine derivatives obtained by different methods in Nellore cattle","attachmentId":73479612,"attachmentType":"pdf","work_url":"https://www.academia.edu/59683647/Endogenous_fraction_and_urinary_recovery_of_purine_derivatives_obtained_by_different_methods_in_Nellore_cattle","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/59683647/Endogenous_fraction_and_urinary_recovery_of_purine_derivatives_obtained_by_different_methods_in_Nellore_cattle"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="68069664" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/68069664/Urinary_excretion_of_purine_derivatives_in_dry_buffalo_and_Fresian_cows">Urinary excretion of purine derivatives in dry buffalo and Fresian cows</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32962053" href="https://unina.academia.edu/MonicaCutrignelli">Monica Cutrignelli</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Italian Journal of …, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives in dry buffalo and Fresian cows","attachmentId":78679142,"attachmentType":"pdf","work_url":"https://www.academia.edu/68069664/Urinary_excretion_of_purine_derivatives_in_dry_buffalo_and_Fresian_cows","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/68069664/Urinary_excretion_of_purine_derivatives_in_dry_buffalo_and_Fresian_cows"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="17908221" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/17908221/Excretion_of_Purine_Derivatives_by_Holstein_Cows_Abomasally_Infused_with_Incremental_Amounts_of_Purines">Excretion of Purine Derivatives by Holstein Cows Abomasally Infused with Incremental Amounts of Purines</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37823538" href="https://independent.academia.edu/RonaldHatfield">Ronald Hatfield</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Dairy Science, 1997</p><p class="ds-related-work--abstract ds2-5-body-sm">Five multiparous, ruminally cannulated Holstein cows (two lactating and three dry) weighing (X +/- SD) 667 +/- 35 kg were used to study the effect of abomasal purine infusion on the excretion of purine derivatives. Cows were fed corn silage four times daily at 90% of ad libitum intake (X = 9.16 kg of dry matter/d). Purines were infused into the abomasum as brewer&amp;amp;amp;#39;s yeast suspensions in five incremental amounts (0 to 380 mmol/d) during five experimental periods according to a 5 x 5 Latin square design. Periods were 7 d; purine infusions were conducted during the last 4 d, and urine was collected during the last 3 d of each period. Ruminal purine outflow in all cows was measured during an experimental period immediately preceding and immediately following the five infusion periods and in each cow during the 0-mmol/d infusion period of the experiment. The relationship between total (milk plus urine) daily excretion of purine derivatives (allantoin plus uric acid) and total (abomasal infusion plus ruminal outflow) daily purine flow was quantified by linear regression analysis and was described by the relationship: Y = 0.856X + 103 (r2 = 0.93). The slope (0.856) indicated that 86% of purines that reached the omasum were excreted as purine derivatives. In the two lactating cows, urinary purine derivatives accounted for 98.4% of the total purine derivatives that were excreted. Ruminal flow of microbial CP can be estimated from the CP:purine ratio of ruminal microorganisms and the excretion of purine derivatives.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Excretion of Purine Derivatives by Holstein Cows Abomasally Infused with Incremental Amounts of Purines","attachmentId":42218473,"attachmentType":"pdf","work_url":"https://www.academia.edu/17908221/Excretion_of_Purine_Derivatives_by_Holstein_Cows_Abomasally_Infused_with_Incremental_Amounts_of_Purines","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17908221/Excretion_of_Purine_Derivatives_by_Holstein_Cows_Abomasally_Infused_with_Incremental_Amounts_of_Purines"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="118674175" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/118674175/Urinary_excretion_of_purine_derivatives_as_influenced_by_GFR_and_plasma_retention_of_purines_in_cattle_i_Bos_indicus_i_i_Bos_taurus_i_and_buffalo_i_Bubalus_bubalis_i_bulls">Urinary excretion of purine derivatives as influenced by GFR and plasma retention of purines in cattle (<i>Bos indicus</i> × <i>Bos taurus</i>) and buffalo (<i>Bubalus bubalis</i>) bulls</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="5319447" href="https://ivri.academia.edu/AshokKumarVerma">Ashok Kumar Verma</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Animal and Feed Sciences, 2013</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives as influenced by GFR and plasma retention of purines in cattle (\u003ci\u003eBos indicus\u003c/i\u003e × \u003ci\u003eBos taurus\u003c/i\u003e) and buffalo (\u003ci\u003eBubalus bubalis\u003c/i\u003e) bulls","attachmentId":114244095,"attachmentType":"pdf","work_url":"https://www.academia.edu/118674175/Urinary_excretion_of_purine_derivatives_as_influenced_by_GFR_and_plasma_retention_of_purines_in_cattle_i_Bos_indicus_i_i_Bos_taurus_i_and_buffalo_i_Bubalus_bubalis_i_bulls","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/118674175/Urinary_excretion_of_purine_derivatives_as_influenced_by_GFR_and_plasma_retention_of_purines_in_cattle_i_Bos_indicus_i_i_Bos_taurus_i_and_buffalo_i_Bubalus_bubalis_i_bulls"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="34129885" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats">Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="66982274" href="https://independent.academia.edu/ABelenguer1">A. Belenguer</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Livestock Production Science, 2002</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats","attachmentId":54057147,"attachmentType":"pdf","work_url":"https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":88734355,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":88734355,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_88734355" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="2" data-entity-id="51109462" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/51109462/The_effect_of_exogenous_purine_supply_on_the_endogenous_excretion_of_purine_derivatives_in_the_urine_of_growing_lambs">The effect of exogenous purine supply on the endogenous excretion of purine derivatives in the urine of growing lambs</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="13428366" href="https://independent.academia.edu/MartinShem">Martin Shem</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Animal Science Journal, 2006</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" 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