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class="label">Followers</p><p class="data">2</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">2</p></div></a><a><div class="stat-container js-profile-coauthors" data-broccoli-component="user-info.coauthors-count" data-click-track="profile-expand-user-info-coauthors"><p class="label">Co-authors</p><p class="data">2</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="66982274" href="https://www.academia.edu/Documents/in/Probiotics_and_Prebiotics"><div 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data-props="{"color":"gray","children":["Sheep"]}" data-trace="false" data-dom-id="Pill-react-component-4620795b-eb28-4b6b-997c-b09e36a1143e"></div> <div id="Pill-react-component-4620795b-eb28-4b6b-997c-b09e36a1143e"></div> </a><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="66982274" href="https://www.academia.edu/Documents/in/Ruminant_Nutrition"><div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Ruminant Nutrition"]}" data-trace="false" data-dom-id="Pill-react-component-a190af2d-45a9-4bf2-aebc-dd773cd4d7ea"></div> <div id="Pill-react-component-a190af2d-45a9-4bf2-aebc-dd773cd4d7ea"></div> </a></div></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by A. Belenguer</h3></div><div class="js-work-strip profile--work_container" data-work-id="23877305"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/23877305/Ruminal_fatty_acid_profile_and_fermentation_characteristics_in_ewes_fed_sunflower_and_fish_oils"><img alt="Research paper thumbnail of Ruminal fatty acid profile and fermentation characteristics in ewes fed sunflower and fish oils" class="work-thumbnail" src="https://attachments.academia-assets.com/44267750/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/23877305/Ruminal_fatty_acid_profile_and_fermentation_characteristics_in_ewes_fed_sunflower_and_fish_oils">Ruminal fatty acid profile and fermentation characteristics in ewes fed sunflower and fish oils</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/KevinShingfield">Kevin Shingfield</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ABelenguer1">A. Belenguer</a></span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ffd59528c0c00720275a99ef940b0ad7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44267750,"asset_id":23877305,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44267750/download_file?st=MTczMjQ0OTkxOSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23877305"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23877305"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23877305; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23877305]").text(description); $(".js-view-count[data-work-id=23877305]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23877305; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23877305']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 23877305, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ffd59528c0c00720275a99ef940b0ad7" } } $('.js-work-strip[data-work-id=23877305]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23877305,"title":"Ruminal fatty acid profile and fermentation characteristics in ewes fed sunflower and fish oils","translated_title":"","metadata":{},"translated_abstract":null,"internal_url":"https://www.academia.edu/23877305/Ruminal_fatty_acid_profile_and_fermentation_characteristics_in_ewes_fed_sunflower_and_fish_oils","translated_internal_url":"","created_at":"2016-03-31T11:06:46.397-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46146532,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18200561,"work_id":23877305,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":2078905,"email":"h***s@eae.csic.es","display_order":0,"name":"G. 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, cons...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen ferment...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23877302"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23877302"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23877302; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23877302]").text(description); $(".js-view-count[data-work-id=23877302]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23877302; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23877302']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 23877302, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=23877302]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23877302,"title":"Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes","translated_title":"","metadata":{"abstract":"Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen ferment...","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Journal of dairy science"},"translated_abstract":"Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen ferment...","internal_url":"https://www.academia.edu/23877302/Oral_administration_of_cobalt_acetate_alters_milk_fatty_acid_composition_consistent_with_an_inhibition_of_stearoyl_coenzyme_A_desaturase_in_lactating_ewes","translated_internal_url":"","created_at":"2016-03-31T11:06:45.558-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46146532,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18200558,"work_id":23877302,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":2078905,"email":"h***s@eae.csic.es","display_order":0,"name":"G. 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Belenguer","title":"Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes"},{"id":18200619,"work_id":23877302,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":4157571,"email":"e***s@eez.csic.es","display_order":7340032,"name":"Eva Ramos-morales","title":"Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes"}],"downloadable_attachments":[],"slug":"Oral_administration_of_cobalt_acetate_alters_milk_fatty_acid_composition_consistent_with_an_inhibition_of_stearoyl_coenzyme_A_desaturase_in_lactating_ewes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46146532,"first_name":"Kevin","middle_initials":null,"last_name":"Shingfield","page_name":"KevinShingfield","domain_name":"independent","created_at":"2016-03-31T11:06:25.101-07:00","display_name":"Kevin Shingfield","url":"https://independent.academia.edu/KevinShingfield"},"attachments":[],"research_interests":[{"id":4630,"name":"Dairy Science","url":"https://www.academia.edu/Documents/in/Dairy_Science"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129892"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129892/Effect_of_the_inclusion_of_quebracho_tannins_in_a_diet_rich_in_linoleic_acid_on_milk_fatty_acid_composition_in_dairy_ewes"><img alt="Research paper thumbnail of Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129892/Effect_of_the_inclusion_of_quebracho_tannins_in_a_diet_rich_in_linoleic_acid_on_milk_fatty_acid_composition_in_dairy_ewes">Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes</a></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20 g of sunflower oil/kg of dry matter plus 0 (control diet) or 20 g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentr...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129892"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129892"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129892; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129892]").text(description); $(".js-view-count[data-work-id=34129892]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129892; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129892']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129892, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129892]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129892,"title":"Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes","translated_title":"","metadata":{"abstract":"Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20 g of sunflower oil/kg of dry matter plus 0 (control diet) or 20 g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentr...","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Journal of dairy science"},"translated_abstract":"Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20 g of sunflower oil/kg of dry matter plus 0 (control diet) or 20 g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentr...","internal_url":"https://www.academia.edu/34129892/Effect_of_the_inclusion_of_quebracho_tannins_in_a_diet_rich_in_linoleic_acid_on_milk_fatty_acid_composition_in_dairy_ewes","translated_internal_url":"","created_at":"2017-08-04T04:20:30.147-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938384,"work_id":34129892,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465909,"email":"p***k@ole.com","display_order":0,"name":"P. 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for lowering saturated fatty acid (FA) content and increasing the concentration of cis-9,trans-11 conjugated linoleic acid and long-chain n-3 FA in ruminant milk. However, changes in populations of ruminal microorganisms associated with altered biohydrogenation of dietary unsaturated FA are not well characterized. Twenty-five lactating Assaf ewes were allocated at random to 1 of 5 treatments composed of dehydrated alfalfa hay and concentrates containing no additional lipid (control), or supplemented with 25 g of sunflower oil and 0 (SO), 8 (SOMA(1)), 16 (SOMA(2)), or 24 (SOMA(3)) g of marine algae/kg of diet dry matter. On d 28 on diet, samples of rumen fluid were collected for lipid analysis and microbial DNA extraction. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas chromatography and Ag+-HPLC analysis of FA methyl esters. Total ba...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23877299"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23877299"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23877299; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23877299]").text(description); $(".js-view-count[data-work-id=23877299]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23877299; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23877299']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 23877299, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=23877299]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23877299,"title":"Fatty acid composition and bacterial community changes in the rumen fluid of lactating sheep fed sunflower oil plus incremental levels of marine algae","translated_title":"","metadata":{"abstract":"Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for lowering saturated fatty acid (FA) content and increasing the concentration of cis-9,trans-11 conjugated linoleic acid and long-chain n-3 FA in ruminant milk. However, changes in populations of ruminal microorganisms associated with altered biohydrogenation of dietary unsaturated FA are not well characterized. Twenty-five lactating Assaf ewes were allocated at random to 1 of 5 treatments composed of dehydrated alfalfa hay and concentrates containing no additional lipid (control), or supplemented with 25 g of sunflower oil and 0 (SO), 8 (SOMA(1)), 16 (SOMA(2)), or 24 (SOMA(3)) g of marine algae/kg of diet dry matter. On d 28 on diet, samples of rumen fluid were collected for lipid analysis and microbial DNA extraction. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas chromatography and Ag+-HPLC analysis of FA methyl esters. Total ba...","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Journal of dairy science"},"translated_abstract":"Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for lowering saturated fatty acid (FA) content and increasing the concentration of cis-9,trans-11 conjugated linoleic acid and long-chain n-3 FA in ruminant milk. However, changes in populations of ruminal microorganisms associated with altered biohydrogenation of dietary unsaturated FA are not well characterized. Twenty-five lactating Assaf ewes were allocated at random to 1 of 5 treatments composed of dehydrated alfalfa hay and concentrates containing no additional lipid (control), or supplemented with 25 g of sunflower oil and 0 (SO), 8 (SOMA(1)), 16 (SOMA(2)), or 24 (SOMA(3)) g of marine algae/kg of diet dry matter. On d 28 on diet, samples of rumen fluid were collected for lipid analysis and microbial DNA extraction. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas chromatography and Ag+-HPLC analysis of FA methyl esters. Total ba...","internal_url":"https://www.academia.edu/23877299/Fatty_acid_composition_and_bacterial_community_changes_in_the_rumen_fluid_of_lactating_sheep_fed_sunflower_oil_plus_incremental_levels_of_marine_algae","translated_internal_url":"","created_at":"2016-03-31T11:06:44.966-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46146532,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18200557,"work_id":23877299,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":2078905,"email":"h***s@eae.csic.es","display_order":0,"name":"G. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129891"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129891/A_Comparison_of_Purine_Derivatives_Excretion_with_Conventional_Methods_as_Indices_of_Microbial_Yield_in_Dairy_Cows"><img alt="Research paper thumbnail of A Comparison of Purine Derivatives Excretion with Conventional Methods as Indices of Microbial Yield in Dairy Cows" class="work-thumbnail" src="https://attachments.academia-assets.com/54057152/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129891/A_Comparison_of_Purine_Derivatives_Excretion_with_Conventional_Methods_as_Indices_of_Microbial_Yield_in_Dairy_Cows">A Comparison of Purine Derivatives Excretion with Conventional Methods as Indices of Microbial Yield in Dairy Cows</a></div><div class="wp-workCard_item"><span>Journal of Dairy Science</span><span>, 2004</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ed61c48669dbb52f143476937b791fc2" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057152,"asset_id":34129891,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057152/download_file?st=MTczMjQ0OTkyMCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129891"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129891"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129891; 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Duodenal digesta flow was estimated using the dual-phase technique in which Cr-EDTA and Yb-acetate were used as liquid and solid markers, respectively. Microbial N (MN) was estimated using the duodenal flow of purine bases (PB); bacterial isolates from the rumen liquid and solid phases were used as references. Additionally, duodenal flow of PB and MN were estimated indirectly using the excretion of purine derivatives (PD) in urine and milk. Duodenal flow of PB and derived MN tended to decrease with feed restriction (from 258 to 154 mmol/ d and 123.5 to 74.4 g/d, respectively). Estimates of PB and MN based on urinary PD showed the same trend, and decreases in PB (from 314 to 266 mmol/d, using LABORATORY [Au: Use of LABORATORY is never defined. Please advise.]) were statistically significant. Using LABORATORY, efficiencies of microbial protein synthesis in the ad libitum treatment were 12.9 and 17.0 g of MN/g of organic matter apparently digested in the rumen when estimated using duodenal PB and urinary excretion of PD, respectively. Urinary excretion of PD closely reflected changes in duodenal flow of PB as a result of feed restriction. 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Johnstone","title":"Impact of high-protein, low-and moderate-carbohydrate diets on the microbal community and on metabolite concentration in faeces: possible implications for gut health"}],"downloadable_attachments":[],"slug":"Impact_of_high_protein_low_and_moderate_carbohydrate_diets_on_the_microbal_community_and_on_metabolite_concentration_in_faeces_possible_implications_for_gut_health","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129888"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129888/Protein_recycling_in_growing_rabbits_contribution_of_microbial_lysine_to_amino_acid_metabolism"><img alt="Research paper thumbnail of Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism" class="work-thumbnail" src="https://attachments.academia-assets.com/54057156/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129888/Protein_recycling_in_growing_rabbits_contribution_of_microbial_lysine_to_amino_acid_metabolism">Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism</a></div><div class="wp-workCard_item"><span>British Journal of Nutrition</span><span>, 2005</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="45703f721cd24fd3568b68e1fd737ecb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057156,"asset_id":34129888,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057156/download_file?st=MTczMjQ0OTkyMCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129888"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129888"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129888; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129888]").text(description); $(".js-view-count[data-work-id=34129888]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129888; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129888']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129888, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "45703f721cd24fd3568b68e1fd737ecb" } } $('.js-work-strip[data-work-id=34129888]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129888,"title":"Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism","translated_title":"","metadata":{"grobid_abstract":"To study the absorption of microbial lysine in growing rabbits, a labelled diet (supplemented with 15 NH 4 Cl) was administered to six animals (group ISOT); a control group (CTRL, four rabbits) received a similar, but unlabelled, diet. Diets were administered for 30 d. An additional group of six animals were fed the unlabelled diet for 20 d and then the labelled diet for 10 d while wearing a neck collar to avoid caecotrophy (group COLL), in order to discriminate it from direct intestinal absorption. At day 30 animals were slaughtered and caecal bacteria and liver samples taken. The 15 N enrichment in amino acids of caecal bacteria and liver were determined by GC -combustion/isotope ratio MS. Lysine showed a higher enrichment in caecal microflora (0·925 atom% excess, APE) than liver (0·215 APE) in group ISOT animals, confirming the double origin of body lysine: microbial and dietary. The COLL group showed a much lower enrichment in tissue lysine (0·007 (SE 0·0029) APE for liver). Any enrichment in the latter animals was due to direct absorption of microbial lysine along the digestive tract, since recycling of microbial protein (caecotrophy) was avoided. 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Rabbit: Caecotrophy: Microbial lysine: 15 N kinetics * Corresponding author: Dr Joaquim Balcells, fax þ 34 976 761590, email balcells@unizar.es","publication_date":{"day":null,"month":null,"year":2005,"errors":{}},"publication_name":"British Journal of Nutrition","grobid_abstract_attachment_id":54057156},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129888/Protein_recycling_in_growing_rabbits_contribution_of_microbial_lysine_to_amino_acid_metabolism","translated_internal_url":"","created_at":"2017-08-04T04:20:29.484-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938386,"work_id":34129888,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism"},{"id":29938391,"work_id":34129888,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465911,"email":"m***x@novusint.com","display_order":4194304,"name":"Marc Decoux","title":"Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism"}],"downloadable_attachments":[{"id":54057156,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057156/thumbnails/1.jpg","file_name":"Protein_recycling_in_growing_rabbits_Con20170804-8908-9edlz8.pdf","download_url":"https://www.academia.edu/attachments/54057156/download_file?st=MTczMjQ0OTkyMCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Protein_recycling_in_growing_rabbits_con.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057156/Protein_recycling_in_growing_rabbits_Con20170804-8908-9edlz8-libre.pdf?1501846135=\u0026response-content-disposition=attachment%3B+filename%3DProtein_recycling_in_growing_rabbits_con.pdf\u0026Expires=1732453520\u0026Signature=XWJmb6VKeuJbGGAwvDxZBZiAXPeFi~N0-POZsU0U0SLH9qKElOlxox2~ftvkyJNszcfJk3UkMWzFCIq8TfW9CwXkd5NrWenmJZQMGi6FmLqrljEqdK9MyNHhQksP9K8FFiXcJ-fbWnVsqtJsDJtpSAQ6D6-kEFvrQRiw~kMBI~gFFUVCcOsTnbgw3lQGE~OgSXLXLovrmGAiFa9fSoLZwL8RqsS4OndZA0I0ajOm8HZCsN~diiGz2jd-k3x4qv9ou46mwiq2TaMcGMHKn6hkRyMP8CdXQaKcswHxl058iFfHeaOdpM7p2NJUpck2LE5yLqz8Cgp165ERQd0qXn6CGg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Protein_recycling_in_growing_rabbits_contribution_of_microbial_lysine_to_amino_acid_metabolism","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Small Ruminant Research</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ec399133d6a67bea9817bc58bec73cbb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":49571499,"asset_id":29124942,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/49571499/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="29124942"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="29124942"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 29124942; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=29124942]").text(description); $(".js-view-count[data-work-id=29124942]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 29124942; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='29124942']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 29124942, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ec399133d6a67bea9817bc58bec73cbb" } } $('.js-work-strip[data-work-id=29124942]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":29124942,"title":"Effect of the supplementation of a high-concentrate diet with sunflower and fish oils on ruminal fermentation in sheep","translated_title":"","metadata":{"grobid_abstract":"This study was conducted to test the hypothesis that the supplementation of a highconcentrate diet with lipids, reportedly a good strategy for improving the nutritional value of ruminant-derived products, may not necessarily be associated with detrimental effects on ruminal fermentation in sheep. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129887"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129887/Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits"><img alt="Research paper thumbnail of Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits" class="work-thumbnail" src="https://attachments.academia-assets.com/54057148/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129887/Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits">Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits</a></div><div class="wp-workCard_item"><span>Livestock Science</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3f22b2b466f3a5b011c12104e74d3918" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057148,"asset_id":34129887,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057148/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129887"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129887"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129887; 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In a first experiment sixteen New Zealand White male rabbits were divided in three groups receiving the same diet, but supplemented with 15 NH 4 Cl in the first group (T 1 : 6 rabbits). The second group (T 2 : 6 rabbits) was also fed the labelled diet but only during the last ten days of the fattening period when animals were fitted a neck collar to prevent caecotrophy. The third group (T 3 : 4 animals) received the basal diet and was used as control. In two additional trials the daily contribution to urinary excretion of endogenous purine compounds (469 ± 50.8 μmol/W 0.75 ) and creatinine excretion (807 ± 127.6 μmol/W 0.75 ) were determined. The highest estimation of microbial nitrogen recycling was obtained by the urinary PD method (0.79 ± 0.096 g/d), whereas caecotrophes collection and 15 N-lysine incorporation methods showed similar values (0.49 ± 0.049 and 0.45 ± 0.015 g/d, respectively). Our results seem to indicate an overestimation of microbial nitrogen recycling in growing rabbits by PD methodology, while neck collar fitting procedure gave similar results, although more variable than microbial 15 N-lysine incorporation.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Livestock Science","grobid_abstract_attachment_id":54057148},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129887/Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits","translated_internal_url":"","created_at":"2017-08-04T04:20:29.319-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938388,"work_id":34129887,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits"}],"downloadable_attachments":[{"id":54057148,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057148/thumbnails/1.jpg","file_name":"j.livsci.2007.06.00220170804-8908-rfg1og.pdf","download_url":"https://www.academia.edu/attachments/54057148/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Alternative_methodologies_to_estimate_in.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057148/j.livsci.2007.06.00220170804-8908-rfg1og-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DAlternative_methodologies_to_estimate_in.pdf\u0026Expires=1732453521\u0026Signature=a0uUjZlN6l17FAvUglnSH-JQAPk4Gq00q~pNh0-00SrMVPcU9OlTrviqXYdVvV3YFt8Ge3vZrHyh~6rhPfpYnTsIVfNvVulMbmUIii7zc0Iq5GDUioTjPnfRKfxthldCxwEWFUhf6y~0gVFGI8Qs4wawK8HRzs~6hgYkV0CP8VE8tugDgHir9Nu1-rtOV7DJK5ZPgO4TvK7qimT8Nk~4S52BgQwSkFUKcYw~UbgH5PTM4my8R5wEsmjTjoWztuQe3uHQnu0CQmr7N5KkCaRtc0PpjwrsKuaMnMVl-saaJKmG7ewEllzRQbK9hCmaVODw~ZZDb4CaBuq4Lg3CHIraaw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits","translated_slug":"","page_count":7,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[{"id":54057148,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057148/thumbnails/1.jpg","file_name":"j.livsci.2007.06.00220170804-8908-rfg1og.pdf","download_url":"https://www.academia.edu/attachments/54057148/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Alternative_methodologies_to_estimate_in.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057148/j.livsci.2007.06.00220170804-8908-rfg1og-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DAlternative_methodologies_to_estimate_in.pdf\u0026Expires=1732453521\u0026Signature=a0uUjZlN6l17FAvUglnSH-JQAPk4Gq00q~pNh0-00SrMVPcU9OlTrviqXYdVvV3YFt8Ge3vZrHyh~6rhPfpYnTsIVfNvVulMbmUIii7zc0Iq5GDUioTjPnfRKfxthldCxwEWFUhf6y~0gVFGI8Qs4wawK8HRzs~6hgYkV0CP8VE8tugDgHir9Nu1-rtOV7DJK5ZPgO4TvK7qimT8Nk~4S52BgQwSkFUKcYw~UbgH5PTM4my8R5wEsmjTjoWztuQe3uHQnu0CQmr7N5KkCaRtc0PpjwrsKuaMnMVl-saaJKmG7ewEllzRQbK9hCmaVODw~ZZDb4CaBuq4Lg3CHIraaw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129886"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129886/Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits"><img alt="Research paper thumbnail of Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129886/Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits">Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits</a></div><div class="wp-workCard_item"><span>Livestock Science</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate availa...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, thirty-two New Zealand growing male rabbits were randomly assigned to four diets formulated using two sources of structural carbohydrates (fibre), alfalfa hay (AH) and sugar beet pulp (SBP), combined with two sources of non-structural carbohydrates (starch), wheat or maize, at a constant fibre/grain sources ratio (0.80/0.20). Microbial N intake was estimated by preventing caecotrophy with a neck collar and, indirectly, by using urinary purine derivative (PD) excretion and microbial 15N-lysine incorporation. No effect of diet on growth was detected (average growth rate 26.6±0.69 g/d), although dry matter (DM) intake was greater in animals fed diets with AH as main source of fibre than those receiving SBP (100.2 vs. 90.1 g/d; P&amp;lt;0.01). Nonetheless, the latter diets were better digested and no significant differences were observed in digestible organic matter (OM) intake. Between sources of starch, digestibility of DM, OM and N was greater with wheat than maize (P&amp;lt;0.05). Microbial activity in the caecum was stimulated by SBP diets, as indicated by a greater volatile fatty acid concentration (89.6 vs. 67.5 mmol/L; P&amp;lt;0.01) and a lower pH (5.7 vs. 6.2; P&amp;lt;0.001). Significantly higher amino acid 15N-enrichments in both caecotrophes and liver were observed with SBP diets and also in maize-fed rabbits when SBP was the main fibre source. However, microbial contribution to tissue amino acids (0.37±0.008) was not affected by the type of fibre.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129886"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129886"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129886; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129886]").text(description); $(".js-view-count[data-work-id=34129886]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129886; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129886']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129886, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129886]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129886,"title":"Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits","translated_title":"","metadata":{"abstract":"ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, thirty-two New Zealand growing male rabbits were randomly assigned to four diets formulated using two sources of structural carbohydrates (fibre), alfalfa hay (AH) and sugar beet pulp (SBP), combined with two sources of non-structural carbohydrates (starch), wheat or maize, at a constant fibre/grain sources ratio (0.80/0.20). Microbial N intake was estimated by preventing caecotrophy with a neck collar and, indirectly, by using urinary purine derivative (PD) excretion and microbial 15N-lysine incorporation. No effect of diet on growth was detected (average growth rate 26.6±0.69 g/d), although dry matter (DM) intake was greater in animals fed diets with AH as main source of fibre than those receiving SBP (100.2 vs. 90.1 g/d; P\u0026amp;lt;0.01). Nonetheless, the latter diets were better digested and no significant differences were observed in digestible organic matter (OM) intake. Between sources of starch, digestibility of DM, OM and N was greater with wheat than maize (P\u0026amp;lt;0.05). Microbial activity in the caecum was stimulated by SBP diets, as indicated by a greater volatile fatty acid concentration (89.6 vs. 67.5 mmol/L; P\u0026amp;lt;0.01) and a lower pH (5.7 vs. 6.2; P\u0026amp;lt;0.001). Significantly higher amino acid 15N-enrichments in both caecotrophes and liver were observed with SBP diets and also in maize-fed rabbits when SBP was the main fibre source. However, microbial contribution to tissue amino acids (0.37±0.008) was not affected by the type of fibre.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Livestock Science"},"translated_abstract":"ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, thirty-two New Zealand growing male rabbits were randomly assigned to four diets formulated using two sources of structural carbohydrates (fibre), alfalfa hay (AH) and sugar beet pulp (SBP), combined with two sources of non-structural carbohydrates (starch), wheat or maize, at a constant fibre/grain sources ratio (0.80/0.20). Microbial N intake was estimated by preventing caecotrophy with a neck collar and, indirectly, by using urinary purine derivative (PD) excretion and microbial 15N-lysine incorporation. No effect of diet on growth was detected (average growth rate 26.6±0.69 g/d), although dry matter (DM) intake was greater in animals fed diets with AH as main source of fibre than those receiving SBP (100.2 vs. 90.1 g/d; P\u0026amp;lt;0.01). Nonetheless, the latter diets were better digested and no significant differences were observed in digestible organic matter (OM) intake. Between sources of starch, digestibility of DM, OM and N was greater with wheat than maize (P\u0026amp;lt;0.05). Microbial activity in the caecum was stimulated by SBP diets, as indicated by a greater volatile fatty acid concentration (89.6 vs. 67.5 mmol/L; P\u0026amp;lt;0.01) and a lower pH (5.7 vs. 6.2; P\u0026amp;lt;0.001). Significantly higher amino acid 15N-enrichments in both caecotrophes and liver were observed with SBP diets and also in maize-fed rabbits when SBP was the main fibre source. However, microbial contribution to tissue amino acids (0.37±0.008) was not affected by the type of fibre.","internal_url":"https://www.academia.edu/34129886/Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits","translated_internal_url":"","created_at":"2017-08-04T04:20:29.236-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938390,"work_id":34129886,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits"}],"downloadable_attachments":[],"slug":"Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129885"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats"><img alt="Research paper thumbnail of Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats" class="work-thumbnail" src="https://attachments.academia-assets.com/54057147/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats">Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats</a></div><div class="wp-workCard_item"><span>Livestock Production Science</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f65619f4614eda7143fc5484515a5c75" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057147,"asset_id":34129885,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057147/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129885"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129885"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129885; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129885]").text(description); $(".js-view-count[data-work-id=34129885]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129885; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129885']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129885, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f65619f4614eda7143fc5484515a5c75" } } $('.js-work-strip[data-work-id=34129885]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129885,"title":"Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats","translated_title":"","metadata":{"grobid_abstract":"The present study examined the relationship between duodenal flow of purine bases and urinary excretion of their derivatives (i.e., Allantoin, uric acid, xanthine and hypoxanthine) in selected milk goats. Three adult Granadina goats fitted with a T-shaped cannula in the abomasum were used to determine the endogenous contribution to renal excretion of purine derivatives and urinary recovery of abomasaly infused purine bases as yeast-RNA. Animals were fed alfalfa at maintenance 0.75 level. The endogenous contribution of purine derivatives was determined at fasting (11.34 mg N / W or 202.2 0.75 mmol / W ) and it was similar to that obtained in sheep but lower than that reported in cattle. Urinary PD excretion responded linearly to incremental supply of purine bases throughout the abomasal cannula, these recovery (%) averaged 76. Xanthine oxidase activities in goat tissues were, in plasma 0.001 (S.E. 0.0001) i.u. / ml, in liver 0.12 (S.E. 0.021) i.u. / g and in duodenum 0.0009 (S.E. 0.00026) i.u. / g. Again, activities were lower than those detected in cows but close to values determined in sheep. A similar response model between both species (sheep and goat) is suggested. ","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"Livestock Production Science","grobid_abstract_attachment_id":54057147},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats","translated_internal_url":"","created_at":"2017-08-04T04:20:29.140-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938385,"work_id":34129885,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats"}],"downloadable_attachments":[{"id":54057147,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057147/thumbnails/1.jpg","file_name":"Belenguer.pdf","download_url":"https://www.academia.edu/attachments/54057147/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Urinary_excretion_of_purine_derivatives.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057147/Belenguer-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DUrinary_excretion_of_purine_derivatives.pdf\u0026Expires=1732453521\u0026Signature=L3K34yVdIW~vhbOs53~kwdciesZZU6Dz-kxhfcDKaIq8Tb9F6SUQdBvCBTlYPzLi6MQh8Y58Qkh1ZmuHL0eD~6cQDRO7DDUuzXcr3qHo1NK5IPuRRzjk20z1WzNaA02gAxT9J2UM7Eax1pvVdUywOMXej~MM3Kl1w3F6cZV0BGUCymmD8-FVkTWkgxwNTlatjAQ6PU-wJbrhYIoiY1BoilkxsLPI5brXoFgiP73a3FhuSNQMUGOVSG3CljxwvouiYtmbEtHhEZ8z64TA53iVjGeub18cQdlFlSJmntaUIihEE4IL65V4DG~aoTfTaTBJoLwuLuobio0Olbxa5OB9YQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats","translated_slug":"","page_count":9,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. 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In Experiment 1, six bulls were fed up to 5 kg fresh matter (FM) of OL per animal and day for 14 days. In Experiment 2, another six bulls were first subjected to severe feed restriction for 8 days and then fed a higher amount of OL (approximately 10 kg FM daily) for 3 days. In Experiment 3, three bulls received the same amount of OL as in Experiment 1 for 6 days, but adding a severe feed restriction as in Experiment 2. In situ DM disappearance of grass hay and OL, and pH and ammonia and volatile fatty acid concentrations were recorded throughout the three assays. Daily administration of up to 5 kg OL did not considerably affect ruminal fermentation, unless it was preceded by a severe feed restriction period. Administration of 10 kg OL preceded by undernutrition triggered a critical reduction in rumen fermentation activity concomitantly with an acute intoxication. Interestingly, some results differ from those observed previously in vitro, which highlights the importance of validating in vitro data with in vivo measurements, given the complexity of extrapolation in ruminants.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129884"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129884"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129884; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129884]").text(description); $(".js-view-count[data-work-id=34129884]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129884; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129884']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129884, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129884]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129884,"title":"Effect of the administration of young leaves of Quercus pyrenaica on rumen fermentation in relation to oak tannin toxicosis in cattle","translated_title":"","metadata":{"abstract":"Three experiments were conducted to study the effect of the administration of young leaves of Quercus pyrenaica (OL) on in vivo ruminal fermentation in relation to oak tannin toxicosis in cattle. In Experiment 1, six bulls were fed up to 5 kg fresh matter (FM) of OL per animal and day for 14 days. In Experiment 2, another six bulls were first subjected to severe feed restriction for 8 days and then fed a higher amount of OL (approximately 10 kg FM daily) for 3 days. In Experiment 3, three bulls received the same amount of OL as in Experiment 1 for 6 days, but adding a severe feed restriction as in Experiment 2. In situ DM disappearance of grass hay and OL, and pH and ammonia and volatile fatty acid concentrations were recorded throughout the three assays. Daily administration of up to 5 kg OL did not considerably affect ruminal fermentation, unless it was preceded by a severe feed restriction period. Administration of 10 kg OL preceded by undernutrition triggered a critical reduction in rumen fermentation activity concomitantly with an acute intoxication. Interestingly, some results differ from those observed previously in vitro, which highlights the importance of validating in vitro data with in vivo measurements, given the complexity of extrapolation in ruminants.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Journal of Animal Physiology and Animal Nutrition"},"translated_abstract":"Three experiments were conducted to study the effect of the administration of young leaves of Quercus pyrenaica (OL) on in vivo ruminal fermentation in relation to oak tannin toxicosis in cattle. In Experiment 1, six bulls were fed up to 5 kg fresh matter (FM) of OL per animal and day for 14 days. In Experiment 2, another six bulls were first subjected to severe feed restriction for 8 days and then fed a higher amount of OL (approximately 10 kg FM daily) for 3 days. In Experiment 3, three bulls received the same amount of OL as in Experiment 1 for 6 days, but adding a severe feed restriction as in Experiment 2. In situ DM disappearance of grass hay and OL, and pH and ammonia and volatile fatty acid concentrations were recorded throughout the three assays. Daily administration of up to 5 kg OL did not considerably affect ruminal fermentation, unless it was preceded by a severe feed restriction period. Administration of 10 kg OL preceded by undernutrition triggered a critical reduction in rumen fermentation activity concomitantly with an acute intoxication. Interestingly, some results differ from those observed previously in vitro, which highlights the importance of validating in vitro data with in vivo measurements, given the complexity of extrapolation in ruminants.","internal_url":"https://www.academia.edu/34129884/Effect_of_the_administration_of_young_leaves_of_Quercus_pyrenaica_on_rumen_fermentation_in_relation_to_oak_tannin_toxicosis_in_cattle","translated_internal_url":"","created_at":"2017-08-04T04:20:29.061-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938381,"work_id":34129884,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465909,"email":"p***k@ole.com","display_order":0,"name":"P. Toral","title":"Effect of the administration of young leaves of Quercus pyrenaica on rumen fermentation in relation to oak tannin toxicosis in cattle"},{"id":29938394,"work_id":34129884,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465914,"email":"r***2@yahoo.es","display_order":4194304,"name":"R. Doce","title":"Effect of the administration of young leaves of Quercus pyrenaica on rumen fermentation in relation to oak tannin toxicosis in cattle"}],"downloadable_attachments":[],"slug":"Effect_of_the_administration_of_young_leaves_of_Quercus_pyrenaica_on_rumen_fermentation_in_relation_to_oak_tannin_toxicosis_in_cattle","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male"},{"id":149268,"name":"Quercus","url":"https://www.academia.edu/Documents/in/Quercus"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":269129,"name":"Fermentation","url":"https://www.academia.edu/Documents/in/Fermentation"},{"id":592625,"name":"Tannins","url":"https://www.academia.edu/Documents/in/Tannins"},{"id":605838,"name":"Animal Nutrition \u0026 Physiology","url":"https://www.academia.edu/Documents/in/Animal_Nutrition_and_Physiology"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129883"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129883/Reduced_Dietary_Intake_of_Carbohydrates_by_Obese_Subjects_Results_in_Decreased_Concentrations_of_Butyrate_and_Butyrate_Producing_Bacteria_in_Feces"><img alt="Research paper thumbnail of Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces" class="work-thumbnail" src="https://attachments.academia-assets.com/54057151/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129883/Reduced_Dietary_Intake_of_Carbohydrates_by_Obese_Subjects_Results_in_Decreased_Concentrations_of_Butyrate_and_Butyrate_Producing_Bacteria_in_Feces">Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces</a></div><div class="wp-workCard_item"><span>Applied and Environmental Microbiology</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="26a6268332b9d03fd73ccaf21d1723d5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057151,"asset_id":34129883,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057151/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129883"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129883"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129883; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129883]").text(description); $(".js-view-count[data-work-id=34129883]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129883; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129883']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129883, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "26a6268332b9d03fd73ccaf21d1723d5" } } $('.js-work-strip[data-work-id=34129883]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129883,"title":"Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces","translated_title":"","metadata":{"grobid_abstract":"Weight loss diets for humans that are based on a high intake of protein but low intake of fermentable carbohydrate may alter microbial activity and bacterial populations in the large intestine and thus impact on gut health. In this study, 19 healthy, obese (body mass index range, 30 to 42) volunteers were given in succession three different diets: maintenance (M) for 3 days (399 g carbohydrate/day) and then high protein/ medium (164 g/day) carbohydrate (HPMC) and high protein/low (24 g/day) carbohydrate (HPLC) each for 4 weeks. Stool samples were collected at the end of each dietary regimen. Total fecal short-chain fatty acids were 114 mM, 74 mM, and 56 mM (P \u003c 0.001) for M, HPMC, and HPLC diets, respectively, and there was a disproportionate reduction in fecal butyrate (18 mM, 9 mM, and 4 mM, respectively; P \u003c 0.001) with decreasing carbohydrate. Major groups of fecal bacteria were monitored using nine 16S rRNA-targeted fluorescence in situ hybridization probes, relative to counts obtained with the broad probe Eub338. No significant change was seen in the relative counts of the bacteroides (Bac303) (mean, 29.6%) or the clostridial cluster XIVa (Erec482, 23.3%), cluster IX (Prop853, 9.3%), or cluster IV (Fprau645, 11.6%; Rbro730 plus Rfla729, 9.3%) groups. In contrast, the Roseburia spp. and Eubacterium rectale subgroup of cluster XIVa (11%, 8%, and 3% for M, HPMC, and HPLC, respectively; P \u003c 0.001) and bifidobacteria (4%, 2.1%, and 1.9%, respectively; P ‫؍‬ 0.026) decreased as carbohydrate intake decreased. The abundance of butyrate-producing bacteria related to Roseburia spp. and E. rectale correlated well with the decline in fecal butyrate.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Applied and Environmental Microbiology","grobid_abstract_attachment_id":54057151},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129883/Reduced_Dietary_Intake_of_Carbohydrates_by_Obese_Subjects_Results_in_Decreased_Concentrations_of_Butyrate_and_Butyrate_Producing_Bacteria_in_Feces","translated_internal_url":"","created_at":"2017-08-04T04:20:28.965-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938397,"work_id":34129883,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465915,"email":"a***e@redcommerce.com","display_order":0,"name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129882"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129882/Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut"><img alt="Research paper thumbnail of Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human Gut" class="work-thumbnail" src="https://attachments.academia-assets.com/54057145/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129882/Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut">Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human Gut</a></div><div class="wp-workCard_item"><span>Applied and Environmental Microbiology</span><span>, 2006</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0f1412f997f50d68da3388c69e519ff1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057145,"asset_id":34129882,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057145/download_file?st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129882"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129882"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129882; 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Of 12 Bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. The potential for metabolic cross-feeding between Bifidobacterium adolescentis and lactate-utilizing, butyrate-producing Firmicute bacteria related to Eubacterium hallii and Anaerostipes caccae was investigated in vitro. E. hallii L2-7 and A. caccae L1-92 failed to grow on starch in pure culture, but in coculture with B. adolescentis L2-32 butyrate was formed, indicating cross-feeding of metabolites to the lactate utilizers. Studies with [ 13 C]lactate confirmed carbon flow from lactate, via acetyl coenzyme A, to butyrate both in pure cultures of E. hallii and in cocultures with B. adolescentis. Similar results were obtained in cocultures involving B. adolescentis DSM 20083 with fructo-oligosaccharides as the substrate. Butyrate formation was also stimulated, however, in cocultures of B. adolescentis L2-32 grown on starch or fructo-oligosaccharides with Roseburia sp. strain A2-183, which produces butyrate but does not utilize lactate. This is probably a consequence of the release by B. adolescentis of oligosaccharides that are available to Roseburia sp. strain A2-183. We conclude that two distinct mechanisms of metabolic cross-feeding between B. adolescentis and butyrate-forming bacteria may operate in gut ecosystems, one due to consumption of fermentation end products (lactate and acetate) and the other due to cross-feeding of partial breakdown products from complex substrates.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Applied and Environmental Microbiology","grobid_abstract_attachment_id":54057145},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129882/Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut","translated_internal_url":"","created_at":"2017-08-04T04:20:28.847-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938399,"work_id":34129882,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465916,"email":"l***r@univ-lyon2.fr","display_order":0,"name":"P. 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However, its impact on the rumen microbial community and its changes over time, as well as the potential consequences on ruminal function, remain unknown. To examine this impact, five ewes fitted with a ruminal cannula and fed low-quality grass hay were dosed daily with 0.6 mmol of OA/kg body weight through the cannula for 14 days. On days 0 (before the start), 4, 7 and 14 of the administration period, samples of ruminal digesta were collected throughout the day (0, 3, 6 and 9 h after the morning feeding) for analysis of the bacterial community and fermentation parameters (pH, ammonia and volatile fatty acid (VFA) concentrations). In addition, two feedstuffs were incubated in situ using the nylon bag technique to estimate ruminal degradation. Terminal restriction fragment length polymorphism was employed to monitor the dynamics of total bacteria, and quantitative real-time PCR was used to investigate the abundance of the oxalate-degrading Oxalobacter formigenes. Neither pH nor total VFA concentrations were affected. Nevertheless, OA dosing altered molar proportions of most individual VFA and ammonia concentrations (P &amp;lt; 0.001). The dry matter disappearance of alfalfa hay was reduced on days 7 and 14 and that of barley straw only on day 7 (P &amp;lt; 0.01). These slight changes were related to others observed in the relative frequency of a number of terminal restriction fragments. Variations in the ruminal microbiota occurred rapidly with OA administration, which did not modify the bacterial diversity significantly but altered the structure of the community. However, many of these changes were reversed by the end of the experiment, with no significant differences between days 0 and 14 of dosing. These results suggest a rapid adaptation of the rumen bacterial community linked to the estimated increase in the abundance of O. formigenes (from 0.002% to 0.007% of oxc gene in relation to the total bacteria 16S rDNA; P &amp;lt; 0.01), which is assumed to be responsible for oxalate breakdown.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129881"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129881"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129881; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129881]").text(description); $(".js-view-count[data-work-id=34129881]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129881; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129881']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129881, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129881]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129881,"title":"Impact of oxalic acid on rumen function and bacterial community in sheep","translated_title":"","metadata":{"abstract":"Oxalic acid (OA) is a secondary compound occurring in a wide range of plants consumed by ruminants, especially in saline lands or in arid and semi-arid regions. However, its impact on the rumen microbial community and its changes over time, as well as the potential consequences on ruminal function, remain unknown. To examine this impact, five ewes fitted with a ruminal cannula and fed low-quality grass hay were dosed daily with 0.6 mmol of OA/kg body weight through the cannula for 14 days. On days 0 (before the start), 4, 7 and 14 of the administration period, samples of ruminal digesta were collected throughout the day (0, 3, 6 and 9 h after the morning feeding) for analysis of the bacterial community and fermentation parameters (pH, ammonia and volatile fatty acid (VFA) concentrations). In addition, two feedstuffs were incubated in situ using the nylon bag technique to estimate ruminal degradation. Terminal restriction fragment length polymorphism was employed to monitor the dynamics of total bacteria, and quantitative real-time PCR was used to investigate the abundance of the oxalate-degrading Oxalobacter formigenes. Neither pH nor total VFA concentrations were affected. Nevertheless, OA dosing altered molar proportions of most individual VFA and ammonia concentrations (P \u0026amp;lt; 0.001). The dry matter disappearance of alfalfa hay was reduced on days 7 and 14 and that of barley straw only on day 7 (P \u0026amp;lt; 0.01). These slight changes were related to others observed in the relative frequency of a number of terminal restriction fragments. Variations in the ruminal microbiota occurred rapidly with OA administration, which did not modify the bacterial diversity significantly but altered the structure of the community. However, many of these changes were reversed by the end of the experiment, with no significant differences between days 0 and 14 of dosing. These results suggest a rapid adaptation of the rumen bacterial community linked to the estimated increase in the abundance of O. formigenes (from 0.002% to 0.007% of oxc gene in relation to the total bacteria 16S rDNA; P \u0026amp;lt; 0.01), which is assumed to be responsible for oxalate breakdown.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"animal"},"translated_abstract":"Oxalic acid (OA) is a secondary compound occurring in a wide range of plants consumed by ruminants, especially in saline lands or in arid and semi-arid regions. However, its impact on the rumen microbial community and its changes over time, as well as the potential consequences on ruminal function, remain unknown. To examine this impact, five ewes fitted with a ruminal cannula and fed low-quality grass hay were dosed daily with 0.6 mmol of OA/kg body weight through the cannula for 14 days. On days 0 (before the start), 4, 7 and 14 of the administration period, samples of ruminal digesta were collected throughout the day (0, 3, 6 and 9 h after the morning feeding) for analysis of the bacterial community and fermentation parameters (pH, ammonia and volatile fatty acid (VFA) concentrations). In addition, two feedstuffs were incubated in situ using the nylon bag technique to estimate ruminal degradation. Terminal restriction fragment length polymorphism was employed to monitor the dynamics of total bacteria, and quantitative real-time PCR was used to investigate the abundance of the oxalate-degrading Oxalobacter formigenes. Neither pH nor total VFA concentrations were affected. Nevertheless, OA dosing altered molar proportions of most individual VFA and ammonia concentrations (P \u0026amp;lt; 0.001). The dry matter disappearance of alfalfa hay was reduced on days 7 and 14 and that of barley straw only on day 7 (P \u0026amp;lt; 0.01). These slight changes were related to others observed in the relative frequency of a number of terminal restriction fragments. Variations in the ruminal microbiota occurred rapidly with OA administration, which did not modify the bacterial diversity significantly but altered the structure of the community. However, many of these changes were reversed by the end of the experiment, with no significant differences between days 0 and 14 of dosing. These results suggest a rapid adaptation of the rumen bacterial community linked to the estimated increase in the abundance of O. formigenes (from 0.002% to 0.007% of oxc gene in relation to the total bacteria 16S rDNA; P \u0026amp;lt; 0.01), which is assumed to be responsible for oxalate breakdown.","internal_url":"https://www.academia.edu/34129881/Impact_of_oxalic_acid_on_rumen_function_and_bacterial_community_in_sheep","translated_internal_url":"","created_at":"2017-08-04T04:20:28.762-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938383,"work_id":34129881,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465909,"email":"p***k@ole.com","display_order":0,"name":"P. 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The HT are antimicrobial, although some rumen bacteria can resist or degrade them into potentially toxic or harmless metabolites. To study the effect of the administration of HT-rich OL (Quercus pyrenaica) after a severe feed restriction on the rumen bacterial community and monitor the variations in some bacterial groups that are potentially able to resist or metabolize tannins, 3 ruminally cannulated bulls were initially fed grass hay and then subjected to a severe 8-day feed restriction period, before receiving OL for 6 days. Then, the animals were offered again grass hay for 12 more days. Rumen contents were sampled throughout the experiment. Quantitative real-time PCR and terminal restriction fragment length polymorphism (T-RFLP) were used to monitor the bacterial dynamics. Animal 1 was not intoxicated and showed lower relative abundances of Streptococcus bovis initially and after the OL administration than animals 2 and 3, which showed acute signs of intoxication. The genus Prevotella increased its abundance with the OL administration, whereas Selenomonas ruminantium was reduced. 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Hydrolysable tannins can undergo significant transformation in the rumen due to the presence ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... Hydrolysable tannins can undergo significant transformation in the rumen due to the presence of ester bonds that are cleaved by microbial enzymes, and there is sufficient evidence in the literature to support rumen microbial adaptation and degradation of those secondary ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129878"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129878"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129878; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129878]").text(description); $(".js-view-count[data-work-id=34129878]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129878; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129878']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129878, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129878]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129878,"title":"Effect of the administration of young oak (Quercus pyrenaica) leaves to cattle on ruminal fermentation","translated_title":"","metadata":{"abstract":"... 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Rumen microbial biohydrogenation of dietary unsaturated fatty acids has a major effect on the pro...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Rumen microbial biohydrogenation of dietary unsaturated fatty acids has a major effect on the process of developing healthier dairy products. This study aimed to investigate in vivo the effect of diet supplementation with sunflower (SO) and fish (FO) oils on the rumen bacterial community in dairy sheep. First, 32 lactating ewes, divided in 8 lots of 4 animals each (2 lots per treatment), were fed a high-concentrate total mixed ration supplemented with 0, 2% SO, 1% FO, or 2% SO plus 1% FO. After 21 d, rumen fluid samples were taken from each lot for DNA extraction and fluorescence in situ hybridization (FISH) analysis. In a second experiment, 5 cannulated ewes were first fed the same TMR, with the exception of a higher forage level, and then changed to the same diet supplemented with 2% SO plus 1% FO. After 0, 3, and 10 d, rumen content samples were taken for DNA extraction and FISH analysis (fluid). 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, cons...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen ferment...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23877302"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23877302"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23877302; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23877302]").text(description); $(".js-view-count[data-work-id=23877302]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23877302; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23877302']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 23877302, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=23877302]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23877302,"title":"Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes","translated_title":"","metadata":{"abstract":"Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen ferment...","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Journal of dairy science"},"translated_abstract":"Previous investigations have shown that cobalt (Co) modifies milk fat composition in cattle, consistent with an inhibition of stearoyl-coenzyme A desaturase (SCD) activity, but it remains unclear whether other ruminant species are also affected. The present study examined the effects of oral administration of Co acetate on intake, rumen function, and milk production and fatty acid (FA) composition in sheep. Twenty lactating Assaf ewes were allocated into 1 of 4 groups and used in a continuous randomized block design that involved a 15-d adaptation, a 6-d treatment, and a 10-d posttreatment period. During the treatment period, animals received an oral drench supplying 0 (control), 3 (Co3), 6 (Co6), and 9 (Co9) mg of Co/kg of BW per day, administered in 3 equal doses at 8-h intervals. Cobalt acetate had no influence on intake or milk fat and protein concentrations, whereas treatments Co6 and Co9 tended to lower milk yield. Results on rumen parameters showed no effects on rumen ferment...","internal_url":"https://www.academia.edu/23877302/Oral_administration_of_cobalt_acetate_alters_milk_fatty_acid_composition_consistent_with_an_inhibition_of_stearoyl_coenzyme_A_desaturase_in_lactating_ewes","translated_internal_url":"","created_at":"2016-03-31T11:06:45.558-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46146532,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18200558,"work_id":23877302,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":2078905,"email":"h***s@eae.csic.es","display_order":0,"name":"G. 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Belenguer","title":"Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes"},{"id":18200619,"work_id":23877302,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":4157571,"email":"e***s@eez.csic.es","display_order":7340032,"name":"Eva Ramos-morales","title":"Oral administration of cobalt acetate alters milk fatty acid composition, consistent with an inhibition of stearoyl-coenzyme A desaturase in lactating ewes"}],"downloadable_attachments":[],"slug":"Oral_administration_of_cobalt_acetate_alters_milk_fatty_acid_composition_consistent_with_an_inhibition_of_stearoyl_coenzyme_A_desaturase_in_lactating_ewes","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46146532,"first_name":"Kevin","middle_initials":null,"last_name":"Shingfield","page_name":"KevinShingfield","domain_name":"independent","created_at":"2016-03-31T11:06:25.101-07:00","display_name":"Kevin Shingfield","url":"https://independent.academia.edu/KevinShingfield"},"attachments":[],"research_interests":[{"id":4630,"name":"Dairy Science","url":"https://www.academia.edu/Documents/in/Dairy_Science"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129892"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129892/Effect_of_the_inclusion_of_quebracho_tannins_in_a_diet_rich_in_linoleic_acid_on_milk_fatty_acid_composition_in_dairy_ewes"><img alt="Research paper thumbnail of Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129892/Effect_of_the_inclusion_of_quebracho_tannins_in_a_diet_rich_in_linoleic_acid_on_milk_fatty_acid_composition_in_dairy_ewes">Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes</a></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20 g of sunflower oil/kg of dry matter plus 0 (control diet) or 20 g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentr...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129892"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129892"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129892; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129892]").text(description); $(".js-view-count[data-work-id=34129892]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129892; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129892']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129892, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129892]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129892,"title":"Effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on milk fatty acid composition in dairy ewes","translated_title":"","metadata":{"abstract":"Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20 g of sunflower oil/kg of dry matter plus 0 (control diet) or 20 g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentr...","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Journal of dairy science"},"translated_abstract":"Despite controversy surrounding the ability of tannins to modulate the fatty acid (FA) profile of ruminant-derived products, reports on this issue are still very limited for dairy sheep. This study was conducted to examine the effect of the inclusion of quebracho tannins in a diet rich in linoleic acid on ewe performance and milk FA composition. Thirty-six lactating ewes were distributed into 6 lots and allocated to 2 treatments (3 lots/treatment): control or quebracho. All sheep received a total mixed ration based on alfalfa hay and a concentrate (forage:concentrate ratio of 40:60) supplemented with 20 g of sunflower oil/kg of dry matter plus 0 (control diet) or 20 g of an extract of quebracho tannins/kg of dry matter (QUE diet). Milk production and composition were analyzed on d 0, 3, 6, 9, 12, 15, 18, 21, 24, and 27 on treatments, and milk FA profile on d 0, 3, 6, 12, 18, and 27. On d 27, samples of rumen fluid were collected for pH, and lactate, ammonia, and volatile FA concentr...","internal_url":"https://www.academia.edu/34129892/Effect_of_the_inclusion_of_quebracho_tannins_in_a_diet_rich_in_linoleic_acid_on_milk_fatty_acid_composition_in_dairy_ewes","translated_internal_url":"","created_at":"2017-08-04T04:20:30.147-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938384,"work_id":34129892,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465909,"email":"p***k@ole.com","display_order":0,"name":"P. 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for lowering saturated fatty acid (FA) content and increasing the concentration of cis-9,trans-11 conjugated linoleic acid and long-chain n-3 FA in ruminant milk. However, changes in populations of ruminal microorganisms associated with altered biohydrogenation of dietary unsaturated FA are not well characterized. Twenty-five lactating Assaf ewes were allocated at random to 1 of 5 treatments composed of dehydrated alfalfa hay and concentrates containing no additional lipid (control), or supplemented with 25 g of sunflower oil and 0 (SO), 8 (SOMA(1)), 16 (SOMA(2)), or 24 (SOMA(3)) g of marine algae/kg of diet dry matter. On d 28 on diet, samples of rumen fluid were collected for lipid analysis and microbial DNA extraction. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas chromatography and Ag+-HPLC analysis of FA methyl esters. Total ba...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="23877299"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="23877299"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23877299; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23877299]").text(description); $(".js-view-count[data-work-id=23877299]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23877299; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='23877299']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 23877299, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=23877299]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":23877299,"title":"Fatty acid composition and bacterial community changes in the rumen fluid of lactating sheep fed sunflower oil plus incremental levels of marine algae","translated_title":"","metadata":{"abstract":"Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for lowering saturated fatty acid (FA) content and increasing the concentration of cis-9,trans-11 conjugated linoleic acid and long-chain n-3 FA in ruminant milk. However, changes in populations of ruminal microorganisms associated with altered biohydrogenation of dietary unsaturated FA are not well characterized. Twenty-five lactating Assaf ewes were allocated at random to 1 of 5 treatments composed of dehydrated alfalfa hay and concentrates containing no additional lipid (control), or supplemented with 25 g of sunflower oil and 0 (SO), 8 (SOMA(1)), 16 (SOMA(2)), or 24 (SOMA(3)) g of marine algae/kg of diet dry matter. On d 28 on diet, samples of rumen fluid were collected for lipid analysis and microbial DNA extraction. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas chromatography and Ag+-HPLC analysis of FA methyl esters. Total ba...","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Journal of dairy science"},"translated_abstract":"Supplementation of ruminant diets with plant oils and marine lipids is an effective strategy for lowering saturated fatty acid (FA) content and increasing the concentration of cis-9,trans-11 conjugated linoleic acid and long-chain n-3 FA in ruminant milk. However, changes in populations of ruminal microorganisms associated with altered biohydrogenation of dietary unsaturated FA are not well characterized. Twenty-five lactating Assaf ewes were allocated at random to 1 of 5 treatments composed of dehydrated alfalfa hay and concentrates containing no additional lipid (control), or supplemented with 25 g of sunflower oil and 0 (SO), 8 (SOMA(1)), 16 (SOMA(2)), or 24 (SOMA(3)) g of marine algae/kg of diet dry matter. On d 28 on diet, samples of rumen fluid were collected for lipid analysis and microbial DNA extraction. Appearance and identification of biohydrogenation intermediates was determined based on complementary gas chromatography and Ag+-HPLC analysis of FA methyl esters. Total ba...","internal_url":"https://www.academia.edu/23877299/Fatty_acid_composition_and_bacterial_community_changes_in_the_rumen_fluid_of_lactating_sheep_fed_sunflower_oil_plus_incremental_levels_of_marine_algae","translated_internal_url":"","created_at":"2016-03-31T11:06:44.966-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46146532,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18200557,"work_id":23877299,"tagging_user_id":46146532,"tagged_user_id":null,"co_author_invite_id":2078905,"email":"h***s@eae.csic.es","display_order":0,"name":"G. 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Duodenal digesta flow was estimated using the dual-phase technique in which Cr-EDTA and Yb-acetate were used as liquid and solid markers, respectively. Microbial N (MN) was estimated using the duodenal flow of purine bases (PB); bacterial isolates from the rumen liquid and solid phases were used as references. Additionally, duodenal flow of PB and MN were estimated indirectly using the excretion of purine derivatives (PD) in urine and milk. Duodenal flow of PB and derived MN tended to decrease with feed restriction (from 258 to 154 mmol/ d and 123.5 to 74.4 g/d, respectively). Estimates of PB and MN based on urinary PD showed the same trend, and decreases in PB (from 314 to 266 mmol/d, using LABORATORY [Au: Use of LABORATORY is never defined. Please advise.]) were statistically significant. Using LABORATORY, efficiencies of microbial protein synthesis in the ad libitum treatment were 12.9 and 17.0 g of MN/g of organic matter apparently digested in the rumen when estimated using duodenal PB and urinary excretion of PD, respectively. Urinary excretion of PD closely reflected changes in duodenal flow of PB as a result of feed restriction. 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Johnstone","title":"Impact of high-protein, low-and moderate-carbohydrate diets on the microbal community and on metabolite concentration in faeces: possible implications for gut health"}],"downloadable_attachments":[],"slug":"Impact_of_high_protein_low_and_moderate_carbohydrate_diets_on_the_microbal_community_and_on_metabolite_concentration_in_faeces_possible_implications_for_gut_health","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[],"research_interests":[],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129888"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129888/Protein_recycling_in_growing_rabbits_contribution_of_microbial_lysine_to_amino_acid_metabolism"><img alt="Research paper thumbnail of Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism" class="work-thumbnail" src="https://attachments.academia-assets.com/54057156/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129888/Protein_recycling_in_growing_rabbits_contribution_of_microbial_lysine_to_amino_acid_metabolism">Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism</a></div><div class="wp-workCard_item"><span>British Journal of Nutrition</span><span>, 2005</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="45703f721cd24fd3568b68e1fd737ecb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057156,"asset_id":34129888,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057156/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129888"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129888"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129888; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129888]").text(description); $(".js-view-count[data-work-id=34129888]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129888; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129888']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129888, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "45703f721cd24fd3568b68e1fd737ecb" } } $('.js-work-strip[data-work-id=34129888]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129888,"title":"Protein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolism","translated_title":"","metadata":{"grobid_abstract":"To study the absorption of microbial lysine in growing rabbits, a labelled diet (supplemented with 15 NH 4 Cl) was administered to six animals (group ISOT); a control group (CTRL, four rabbits) received a similar, but unlabelled, diet. Diets were administered for 30 d. An additional group of six animals were fed the unlabelled diet for 20 d and then the labelled diet for 10 d while wearing a neck collar to avoid caecotrophy (group COLL), in order to discriminate it from direct intestinal absorption. At day 30 animals were slaughtered and caecal bacteria and liver samples taken. The 15 N enrichment in amino acids of caecal bacteria and liver were determined by GC -combustion/isotope ratio MS. Lysine showed a higher enrichment in caecal microflora (0·925 atom% excess, APE) than liver (0·215 APE) in group ISOT animals, confirming the double origin of body lysine: microbial and dietary. The COLL group showed a much lower enrichment in tissue lysine (0·007 (SE 0·0029) APE for liver). Any enrichment in the latter animals was due to direct absorption of microbial lysine along the digestive tract, since recycling of microbial protein (caecotrophy) was avoided. 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Small Ruminant Research</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ec399133d6a67bea9817bc58bec73cbb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":49571499,"asset_id":29124942,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/49571499/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="29124942"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="29124942"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 29124942; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=29124942]").text(description); $(".js-view-count[data-work-id=29124942]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 29124942; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='29124942']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 29124942, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ec399133d6a67bea9817bc58bec73cbb" } } $('.js-work-strip[data-work-id=29124942]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":29124942,"title":"Effect of the supplementation of a high-concentrate diet with sunflower and fish oils on ruminal fermentation in sheep","translated_title":"","metadata":{"grobid_abstract":"This study was conducted to test the hypothesis that the supplementation of a highconcentrate diet with lipids, reportedly a good strategy for improving the nutritional value of ruminant-derived products, may not necessarily be associated with detrimental effects on ruminal fermentation in sheep. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129887"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129887/Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits"><img alt="Research paper thumbnail of Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits" class="work-thumbnail" src="https://attachments.academia-assets.com/54057148/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129887/Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits">Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits</a></div><div class="wp-workCard_item"><span>Livestock Science</span><span>, 2008</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3f22b2b466f3a5b011c12104e74d3918" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057148,"asset_id":34129887,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057148/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129887"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129887"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129887; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129887]").text(description); $(".js-view-count[data-work-id=34129887]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129887; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129887']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129887, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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In a first experiment sixteen New Zealand White male rabbits were divided in three groups receiving the same diet, but supplemented with 15 NH 4 Cl in the first group (T 1 : 6 rabbits). The second group (T 2 : 6 rabbits) was also fed the labelled diet but only during the last ten days of the fattening period when animals were fitted a neck collar to prevent caecotrophy. The third group (T 3 : 4 animals) received the basal diet and was used as control. In two additional trials the daily contribution to urinary excretion of endogenous purine compounds (469 ± 50.8 μmol/W 0.75 ) and creatinine excretion (807 ± 127.6 μmol/W 0.75 ) were determined. The highest estimation of microbial nitrogen recycling was obtained by the urinary PD method (0.79 ± 0.096 g/d), whereas caecotrophes collection and 15 N-lysine incorporation methods showed similar values (0.49 ± 0.049 and 0.45 ± 0.015 g/d, respectively). Our results seem to indicate an overestimation of microbial nitrogen recycling in growing rabbits by PD methodology, while neck collar fitting procedure gave similar results, although more variable than microbial 15 N-lysine incorporation.","publication_date":{"day":null,"month":null,"year":2008,"errors":{}},"publication_name":"Livestock Science","grobid_abstract_attachment_id":54057148},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129887/Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits","translated_internal_url":"","created_at":"2017-08-04T04:20:29.319-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938388,"work_id":34129887,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Alternative methodologies to estimate ingestion of caecotrophes in growing rabbits"}],"downloadable_attachments":[{"id":54057148,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057148/thumbnails/1.jpg","file_name":"j.livsci.2007.06.00220170804-8908-rfg1og.pdf","download_url":"https://www.academia.edu/attachments/54057148/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Alternative_methodologies_to_estimate_in.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057148/j.livsci.2007.06.00220170804-8908-rfg1og-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DAlternative_methodologies_to_estimate_in.pdf\u0026Expires=1732453521\u0026Signature=a0uUjZlN6l17FAvUglnSH-JQAPk4Gq00q~pNh0-00SrMVPcU9OlTrviqXYdVvV3YFt8Ge3vZrHyh~6rhPfpYnTsIVfNvVulMbmUIii7zc0Iq5GDUioTjPnfRKfxthldCxwEWFUhf6y~0gVFGI8Qs4wawK8HRzs~6hgYkV0CP8VE8tugDgHir9Nu1-rtOV7DJK5ZPgO4TvK7qimT8Nk~4S52BgQwSkFUKcYw~UbgH5PTM4my8R5wEsmjTjoWztuQe3uHQnu0CQmr7N5KkCaRtc0PpjwrsKuaMnMVl-saaJKmG7ewEllzRQbK9hCmaVODw~ZZDb4CaBuq4Lg3CHIraaw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Alternative_methodologies_to_estimate_ingestion_of_caecotrophes_in_growing_rabbits","translated_slug":"","page_count":7,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[{"id":54057148,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057148/thumbnails/1.jpg","file_name":"j.livsci.2007.06.00220170804-8908-rfg1og.pdf","download_url":"https://www.academia.edu/attachments/54057148/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Alternative_methodologies_to_estimate_in.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057148/j.livsci.2007.06.00220170804-8908-rfg1og-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DAlternative_methodologies_to_estimate_in.pdf\u0026Expires=1732453521\u0026Signature=a0uUjZlN6l17FAvUglnSH-JQAPk4Gq00q~pNh0-00SrMVPcU9OlTrviqXYdVvV3YFt8Ge3vZrHyh~6rhPfpYnTsIVfNvVulMbmUIii7zc0Iq5GDUioTjPnfRKfxthldCxwEWFUhf6y~0gVFGI8Qs4wawK8HRzs~6hgYkV0CP8VE8tugDgHir9Nu1-rtOV7DJK5ZPgO4TvK7qimT8Nk~4S52BgQwSkFUKcYw~UbgH5PTM4my8R5wEsmjTjoWztuQe3uHQnu0CQmr7N5KkCaRtc0PpjwrsKuaMnMVl-saaJKmG7ewEllzRQbK9hCmaVODw~ZZDb4CaBuq4Lg3CHIraaw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129886"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129886/Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits"><img alt="Research paper thumbnail of Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129886/Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits">Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits</a></div><div class="wp-workCard_item"><span>Livestock Science</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate availa...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, thirty-two New Zealand growing male rabbits were randomly assigned to four diets formulated using two sources of structural carbohydrates (fibre), alfalfa hay (AH) and sugar beet pulp (SBP), combined with two sources of non-structural carbohydrates (starch), wheat or maize, at a constant fibre/grain sources ratio (0.80/0.20). Microbial N intake was estimated by preventing caecotrophy with a neck collar and, indirectly, by using urinary purine derivative (PD) excretion and microbial 15N-lysine incorporation. No effect of diet on growth was detected (average growth rate 26.6±0.69 g/d), although dry matter (DM) intake was greater in animals fed diets with AH as main source of fibre than those receiving SBP (100.2 vs. 90.1 g/d; P&amp;lt;0.01). Nonetheless, the latter diets were better digested and no significant differences were observed in digestible organic matter (OM) intake. Between sources of starch, digestibility of DM, OM and N was greater with wheat than maize (P&amp;lt;0.05). Microbial activity in the caecum was stimulated by SBP diets, as indicated by a greater volatile fatty acid concentration (89.6 vs. 67.5 mmol/L; P&amp;lt;0.01) and a lower pH (5.7 vs. 6.2; P&amp;lt;0.001). Significantly higher amino acid 15N-enrichments in both caecotrophes and liver were observed with SBP diets and also in maize-fed rabbits when SBP was the main fibre source. However, microbial contribution to tissue amino acids (0.37±0.008) was not affected by the type of fibre.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129886"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129886"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129886; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129886]").text(description); $(".js-view-count[data-work-id=34129886]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129886; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129886']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129886, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129886]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129886,"title":"Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits","translated_title":"","metadata":{"abstract":"ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, thirty-two New Zealand growing male rabbits were randomly assigned to four diets formulated using two sources of structural carbohydrates (fibre), alfalfa hay (AH) and sugar beet pulp (SBP), combined with two sources of non-structural carbohydrates (starch), wheat or maize, at a constant fibre/grain sources ratio (0.80/0.20). Microbial N intake was estimated by preventing caecotrophy with a neck collar and, indirectly, by using urinary purine derivative (PD) excretion and microbial 15N-lysine incorporation. No effect of diet on growth was detected (average growth rate 26.6±0.69 g/d), although dry matter (DM) intake was greater in animals fed diets with AH as main source of fibre than those receiving SBP (100.2 vs. 90.1 g/d; P\u0026amp;lt;0.01). Nonetheless, the latter diets were better digested and no significant differences were observed in digestible organic matter (OM) intake. Between sources of starch, digestibility of DM, OM and N was greater with wheat than maize (P\u0026amp;lt;0.05). Microbial activity in the caecum was stimulated by SBP diets, as indicated by a greater volatile fatty acid concentration (89.6 vs. 67.5 mmol/L; P\u0026amp;lt;0.01) and a lower pH (5.7 vs. 6.2; P\u0026amp;lt;0.001). Significantly higher amino acid 15N-enrichments in both caecotrophes and liver were observed with SBP diets and also in maize-fed rabbits when SBP was the main fibre source. However, microbial contribution to tissue amino acids (0.37±0.008) was not affected by the type of fibre.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"Livestock Science"},"translated_abstract":"ABSTRACT In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, thirty-two New Zealand growing male rabbits were randomly assigned to four diets formulated using two sources of structural carbohydrates (fibre), alfalfa hay (AH) and sugar beet pulp (SBP), combined with two sources of non-structural carbohydrates (starch), wheat or maize, at a constant fibre/grain sources ratio (0.80/0.20). Microbial N intake was estimated by preventing caecotrophy with a neck collar and, indirectly, by using urinary purine derivative (PD) excretion and microbial 15N-lysine incorporation. No effect of diet on growth was detected (average growth rate 26.6±0.69 g/d), although dry matter (DM) intake was greater in animals fed diets with AH as main source of fibre than those receiving SBP (100.2 vs. 90.1 g/d; P\u0026amp;lt;0.01). Nonetheless, the latter diets were better digested and no significant differences were observed in digestible organic matter (OM) intake. Between sources of starch, digestibility of DM, OM and N was greater with wheat than maize (P\u0026amp;lt;0.05). Microbial activity in the caecum was stimulated by SBP diets, as indicated by a greater volatile fatty acid concentration (89.6 vs. 67.5 mmol/L; P\u0026amp;lt;0.01) and a lower pH (5.7 vs. 6.2; P\u0026amp;lt;0.001). Significantly higher amino acid 15N-enrichments in both caecotrophes and liver were observed with SBP diets and also in maize-fed rabbits when SBP was the main fibre source. However, microbial contribution to tissue amino acids (0.37±0.008) was not affected by the type of fibre.","internal_url":"https://www.academia.edu/34129886/Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits","translated_internal_url":"","created_at":"2017-08-04T04:20:29.236-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938390,"work_id":34129886,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Effect of carbohydrate source on microbial nitrogen recycling in growing rabbits"}],"downloadable_attachments":[],"slug":"Effect_of_carbohydrate_source_on_microbial_nitrogen_recycling_in_growing_rabbits","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129885"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats"><img alt="Research paper thumbnail of Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats" class="work-thumbnail" src="https://attachments.academia-assets.com/54057147/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats">Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats</a></div><div class="wp-workCard_item"><span>Livestock Production Science</span><span>, 2002</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f65619f4614eda7143fc5484515a5c75" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057147,"asset_id":34129885,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057147/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129885"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129885"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129885; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129885]").text(description); $(".js-view-count[data-work-id=34129885]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129885; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129885']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129885, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f65619f4614eda7143fc5484515a5c75" } } $('.js-work-strip[data-work-id=34129885]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129885,"title":"Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats","translated_title":"","metadata":{"grobid_abstract":"The present study examined the relationship between duodenal flow of purine bases and urinary excretion of their derivatives (i.e., Allantoin, uric acid, xanthine and hypoxanthine) in selected milk goats. Three adult Granadina goats fitted with a T-shaped cannula in the abomasum were used to determine the endogenous contribution to renal excretion of purine derivatives and urinary recovery of abomasaly infused purine bases as yeast-RNA. Animals were fed alfalfa at maintenance 0.75 level. The endogenous contribution of purine derivatives was determined at fasting (11.34 mg N / W or 202.2 0.75 mmol / W ) and it was similar to that obtained in sheep but lower than that reported in cattle. Urinary PD excretion responded linearly to incremental supply of purine bases throughout the abomasal cannula, these recovery (%) averaged 76. Xanthine oxidase activities in goat tissues were, in plasma 0.001 (S.E. 0.0001) i.u. / ml, in liver 0.12 (S.E. 0.021) i.u. / g and in duodenum 0.0009 (S.E. 0.00026) i.u. / g. Again, activities were lower than those detected in cows but close to values determined in sheep. A similar response model between both species (sheep and goat) is suggested. ","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"Livestock Production Science","grobid_abstract_attachment_id":54057147},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129885/Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats","translated_internal_url":"","created_at":"2017-08-04T04:20:29.140-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938385,"work_id":34129885,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465910,"email":"b***s@prodan.udl.cat","display_order":0,"name":"Joaquim Balcells","title":"Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats"}],"downloadable_attachments":[{"id":54057147,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057147/thumbnails/1.jpg","file_name":"Belenguer.pdf","download_url":"https://www.academia.edu/attachments/54057147/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Urinary_excretion_of_purine_derivatives.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057147/Belenguer-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DUrinary_excretion_of_purine_derivatives.pdf\u0026Expires=1732453521\u0026Signature=L3K34yVdIW~vhbOs53~kwdciesZZU6Dz-kxhfcDKaIq8Tb9F6SUQdBvCBTlYPzLi6MQh8Y58Qkh1ZmuHL0eD~6cQDRO7DDUuzXcr3qHo1NK5IPuRRzjk20z1WzNaA02gAxT9J2UM7Eax1pvVdUywOMXej~MM3Kl1w3F6cZV0BGUCymmD8-FVkTWkgxwNTlatjAQ6PU-wJbrhYIoiY1BoilkxsLPI5brXoFgiP73a3FhuSNQMUGOVSG3CljxwvouiYtmbEtHhEZ8z64TA53iVjGeub18cQdlFlSJmntaUIihEE4IL65V4DG~aoTfTaTBJoLwuLuobio0Olbxa5OB9YQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Urinary_excretion_of_purine_derivatives_and_prediction_of_rumen_microbial_outflow_in_goats","translated_slug":"","page_count":9,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. 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In Experiment 1, six bulls were fed up to 5 kg fresh matter (FM) of OL per animal and day for 14 days. In Experiment 2, another six bulls were first subjected to severe feed restriction for 8 days and then fed a higher amount of OL (approximately 10 kg FM daily) for 3 days. In Experiment 3, three bulls received the same amount of OL as in Experiment 1 for 6 days, but adding a severe feed restriction as in Experiment 2. In situ DM disappearance of grass hay and OL, and pH and ammonia and volatile fatty acid concentrations were recorded throughout the three assays. Daily administration of up to 5 kg OL did not considerably affect ruminal fermentation, unless it was preceded by a severe feed restriction period. Administration of 10 kg OL preceded by undernutrition triggered a critical reduction in rumen fermentation activity concomitantly with an acute intoxication. Interestingly, some results differ from those observed previously in vitro, which highlights the importance of validating in vitro data with in vivo measurements, given the complexity of extrapolation in ruminants.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129884"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129884"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129884; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129884]").text(description); $(".js-view-count[data-work-id=34129884]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129884; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129884']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129884, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129884]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129884,"title":"Effect of the administration of young leaves of Quercus pyrenaica on rumen fermentation in relation to oak tannin toxicosis in cattle","translated_title":"","metadata":{"abstract":"Three experiments were conducted to study the effect of the administration of young leaves of Quercus pyrenaica (OL) on in vivo ruminal fermentation in relation to oak tannin toxicosis in cattle. In Experiment 1, six bulls were fed up to 5 kg fresh matter (FM) of OL per animal and day for 14 days. In Experiment 2, another six bulls were first subjected to severe feed restriction for 8 days and then fed a higher amount of OL (approximately 10 kg FM daily) for 3 days. In Experiment 3, three bulls received the same amount of OL as in Experiment 1 for 6 days, but adding a severe feed restriction as in Experiment 2. In situ DM disappearance of grass hay and OL, and pH and ammonia and volatile fatty acid concentrations were recorded throughout the three assays. Daily administration of up to 5 kg OL did not considerably affect ruminal fermentation, unless it was preceded by a severe feed restriction period. Administration of 10 kg OL preceded by undernutrition triggered a critical reduction in rumen fermentation activity concomitantly with an acute intoxication. Interestingly, some results differ from those observed previously in vitro, which highlights the importance of validating in vitro data with in vivo measurements, given the complexity of extrapolation in ruminants.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Journal of Animal Physiology and Animal Nutrition"},"translated_abstract":"Three experiments were conducted to study the effect of the administration of young leaves of Quercus pyrenaica (OL) on in vivo ruminal fermentation in relation to oak tannin toxicosis in cattle. In Experiment 1, six bulls were fed up to 5 kg fresh matter (FM) of OL per animal and day for 14 days. In Experiment 2, another six bulls were first subjected to severe feed restriction for 8 days and then fed a higher amount of OL (approximately 10 kg FM daily) for 3 days. In Experiment 3, three bulls received the same amount of OL as in Experiment 1 for 6 days, but adding a severe feed restriction as in Experiment 2. In situ DM disappearance of grass hay and OL, and pH and ammonia and volatile fatty acid concentrations were recorded throughout the three assays. Daily administration of up to 5 kg OL did not considerably affect ruminal fermentation, unless it was preceded by a severe feed restriction period. Administration of 10 kg OL preceded by undernutrition triggered a critical reduction in rumen fermentation activity concomitantly with an acute intoxication. Interestingly, some results differ from those observed previously in vitro, which highlights the importance of validating in vitro data with in vivo measurements, given the complexity of extrapolation in ruminants.","internal_url":"https://www.academia.edu/34129884/Effect_of_the_administration_of_young_leaves_of_Quercus_pyrenaica_on_rumen_fermentation_in_relation_to_oak_tannin_toxicosis_in_cattle","translated_internal_url":"","created_at":"2017-08-04T04:20:29.061-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938381,"work_id":34129884,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465909,"email":"p***k@ole.com","display_order":0,"name":"P. Toral","title":"Effect of the administration of young leaves of Quercus pyrenaica on rumen fermentation in relation to oak tannin toxicosis in cattle"},{"id":29938394,"work_id":34129884,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465914,"email":"r***2@yahoo.es","display_order":4194304,"name":"R. Doce","title":"Effect of the administration of young leaves of Quercus pyrenaica on rumen fermentation in relation to oak tannin toxicosis in cattle"}],"downloadable_attachments":[],"slug":"Effect_of_the_administration_of_young_leaves_of_Quercus_pyrenaica_on_rumen_fermentation_in_relation_to_oak_tannin_toxicosis_in_cattle","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. Belenguer","url":"https://independent.academia.edu/ABelenguer1"},"attachments":[],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male"},{"id":149268,"name":"Quercus","url":"https://www.academia.edu/Documents/in/Quercus"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":269129,"name":"Fermentation","url":"https://www.academia.edu/Documents/in/Fermentation"},{"id":592625,"name":"Tannins","url":"https://www.academia.edu/Documents/in/Tannins"},{"id":605838,"name":"Animal Nutrition \u0026 Physiology","url":"https://www.academia.edu/Documents/in/Animal_Nutrition_and_Physiology"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129883"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129883/Reduced_Dietary_Intake_of_Carbohydrates_by_Obese_Subjects_Results_in_Decreased_Concentrations_of_Butyrate_and_Butyrate_Producing_Bacteria_in_Feces"><img alt="Research paper thumbnail of Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces" class="work-thumbnail" src="https://attachments.academia-assets.com/54057151/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129883/Reduced_Dietary_Intake_of_Carbohydrates_by_Obese_Subjects_Results_in_Decreased_Concentrations_of_Butyrate_and_Butyrate_Producing_Bacteria_in_Feces">Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces</a></div><div class="wp-workCard_item"><span>Applied and Environmental Microbiology</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="26a6268332b9d03fd73ccaf21d1723d5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057151,"asset_id":34129883,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057151/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129883"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129883"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129883; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129883]").text(description); $(".js-view-count[data-work-id=34129883]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129883; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129883']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129883, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "26a6268332b9d03fd73ccaf21d1723d5" } } $('.js-work-strip[data-work-id=34129883]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129883,"title":"Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces","translated_title":"","metadata":{"grobid_abstract":"Weight loss diets for humans that are based on a high intake of protein but low intake of fermentable carbohydrate may alter microbial activity and bacterial populations in the large intestine and thus impact on gut health. In this study, 19 healthy, obese (body mass index range, 30 to 42) volunteers were given in succession three different diets: maintenance (M) for 3 days (399 g carbohydrate/day) and then high protein/ medium (164 g/day) carbohydrate (HPMC) and high protein/low (24 g/day) carbohydrate (HPLC) each for 4 weeks. Stool samples were collected at the end of each dietary regimen. Total fecal short-chain fatty acids were 114 mM, 74 mM, and 56 mM (P \u003c 0.001) for M, HPMC, and HPLC diets, respectively, and there was a disproportionate reduction in fecal butyrate (18 mM, 9 mM, and 4 mM, respectively; P \u003c 0.001) with decreasing carbohydrate. Major groups of fecal bacteria were monitored using nine 16S rRNA-targeted fluorescence in situ hybridization probes, relative to counts obtained with the broad probe Eub338. No significant change was seen in the relative counts of the bacteroides (Bac303) (mean, 29.6%) or the clostridial cluster XIVa (Erec482, 23.3%), cluster IX (Prop853, 9.3%), or cluster IV (Fprau645, 11.6%; Rbro730 plus Rfla729, 9.3%) groups. In contrast, the Roseburia spp. and Eubacterium rectale subgroup of cluster XIVa (11%, 8%, and 3% for M, HPMC, and HPLC, respectively; P \u003c 0.001) and bifidobacteria (4%, 2.1%, and 1.9%, respectively; P ‫؍‬ 0.026) decreased as carbohydrate intake decreased. The abundance of butyrate-producing bacteria related to Roseburia spp. and E. rectale correlated well with the decline in fecal butyrate.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Applied and Environmental Microbiology","grobid_abstract_attachment_id":54057151},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129883/Reduced_Dietary_Intake_of_Carbohydrates_by_Obese_Subjects_Results_in_Decreased_Concentrations_of_Butyrate_and_Butyrate_Producing_Bacteria_in_Feces","translated_internal_url":"","created_at":"2017-08-04T04:20:28.965-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938397,"work_id":34129883,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465915,"email":"a***e@redcommerce.com","display_order":0,"name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="34129882"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/34129882/Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut"><img alt="Research paper thumbnail of Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human Gut" class="work-thumbnail" src="https://attachments.academia-assets.com/54057145/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/34129882/Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut">Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human Gut</a></div><div class="wp-workCard_item"><span>Applied and Environmental Microbiology</span><span>, 2006</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0f1412f997f50d68da3388c69e519ff1" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54057145,"asset_id":34129882,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54057145/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129882"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129882"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129882; 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Of 12 Bifidobacterium strains of human gut origin from seven species tested, four grew in pure culture on starch and nine on fructo-oligosaccharides. The potential for metabolic cross-feeding between Bifidobacterium adolescentis and lactate-utilizing, butyrate-producing Firmicute bacteria related to Eubacterium hallii and Anaerostipes caccae was investigated in vitro. E. hallii L2-7 and A. caccae L1-92 failed to grow on starch in pure culture, but in coculture with B. adolescentis L2-32 butyrate was formed, indicating cross-feeding of metabolites to the lactate utilizers. Studies with [ 13 C]lactate confirmed carbon flow from lactate, via acetyl coenzyme A, to butyrate both in pure cultures of E. hallii and in cocultures with B. adolescentis. Similar results were obtained in cocultures involving B. adolescentis DSM 20083 with fructo-oligosaccharides as the substrate. Butyrate formation was also stimulated, however, in cocultures of B. adolescentis L2-32 grown on starch or fructo-oligosaccharides with Roseburia sp. strain A2-183, which produces butyrate but does not utilize lactate. This is probably a consequence of the release by B. adolescentis of oligosaccharides that are available to Roseburia sp. strain A2-183. We conclude that two distinct mechanisms of metabolic cross-feeding between B. adolescentis and butyrate-forming bacteria may operate in gut ecosystems, one due to consumption of fermentation end products (lactate and acetate) and the other due to cross-feeding of partial breakdown products from complex substrates.","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"publication_name":"Applied and Environmental Microbiology","grobid_abstract_attachment_id":54057145},"translated_abstract":null,"internal_url":"https://www.academia.edu/34129882/Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut","translated_internal_url":"","created_at":"2017-08-04T04:20:28.847-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938399,"work_id":34129882,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465916,"email":"l***r@univ-lyon2.fr","display_order":0,"name":"P. Louis","title":"Two Routes of Metabolic Cross-Feeding between Bifidobacterium adolescentis and Butyrate-Producing Anaerobes from the Human Gut"}],"downloadable_attachments":[{"id":54057145,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/54057145/thumbnails/1.jpg","file_name":"3593.pdf","download_url":"https://www.academia.edu/attachments/54057145/download_file?st=MTczMjQ0OTkyMiw4LjIyMi4yMDguMTQ2&st=MTczMjQ0OTkyMSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Two_Routes_of_Metabolic_Cross_Feeding_be.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/54057145/3593-libre.pdf?1501845814=\u0026response-content-disposition=attachment%3B+filename%3DTwo_Routes_of_Metabolic_Cross_Feeding_be.pdf\u0026Expires=1732453521\u0026Signature=fTDhikHtoUMr-4sipShRaquaZOeR6~N6fkfX-NU3rMBR-ICD3nG8ny5atLlucFE7PH7aW84rdqDSDkeSC1Hjpf1nQZIjEoadrVcVQd4tMR8IYy0DVyn5hevsn6a5nkOecF6rlYahJcLXWfu9QaNDtz1kcMpkkjm2DlN~ncedZgch6WLRbi2quu3IOsKQ5viOs~qeoCegP9lSD9NaVgdmn5-p~pkJcTCiLLfHi~~z9sWNvTB-L2Y4dUVoKDmIuRnUODups0EbSBm2oRzMrhxl~G0ZyifquuxelYbRfdrqjNWbNebhpXBqiO8APfkAvarZR8401gY1NmQ5iHLlSFgX9g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Two_Routes_of_Metabolic_Cross_Feeding_between_Bifidobacterium_adolescentis_and_Butyrate_Producing_Anaerobes_from_the_Human_Gut","translated_slug":"","page_count":7,"language":"en","content_type":"Work","owner":{"id":66982274,"first_name":"A.","middle_initials":null,"last_name":"Belenguer","page_name":"ABelenguer1","domain_name":"independent","created_at":"2017-08-04T04:17:20.568-07:00","display_name":"A. 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However, its impact on the rumen microbial community and its changes over time, as well as the potential consequences on ruminal function, remain unknown. To examine this impact, five ewes fitted with a ruminal cannula and fed low-quality grass hay were dosed daily with 0.6 mmol of OA/kg body weight through the cannula for 14 days. On days 0 (before the start), 4, 7 and 14 of the administration period, samples of ruminal digesta were collected throughout the day (0, 3, 6 and 9 h after the morning feeding) for analysis of the bacterial community and fermentation parameters (pH, ammonia and volatile fatty acid (VFA) concentrations). In addition, two feedstuffs were incubated in situ using the nylon bag technique to estimate ruminal degradation. Terminal restriction fragment length polymorphism was employed to monitor the dynamics of total bacteria, and quantitative real-time PCR was used to investigate the abundance of the oxalate-degrading Oxalobacter formigenes. Neither pH nor total VFA concentrations were affected. Nevertheless, OA dosing altered molar proportions of most individual VFA and ammonia concentrations (P &amp;lt; 0.001). The dry matter disappearance of alfalfa hay was reduced on days 7 and 14 and that of barley straw only on day 7 (P &amp;lt; 0.01). These slight changes were related to others observed in the relative frequency of a number of terminal restriction fragments. Variations in the ruminal microbiota occurred rapidly with OA administration, which did not modify the bacterial diversity significantly but altered the structure of the community. However, many of these changes were reversed by the end of the experiment, with no significant differences between days 0 and 14 of dosing. These results suggest a rapid adaptation of the rumen bacterial community linked to the estimated increase in the abundance of O. formigenes (from 0.002% to 0.007% of oxc gene in relation to the total bacteria 16S rDNA; P &amp;lt; 0.01), which is assumed to be responsible for oxalate breakdown.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129881"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129881"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129881; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129881]").text(description); $(".js-view-count[data-work-id=34129881]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129881; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129881']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129881, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129881]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129881,"title":"Impact of oxalic acid on rumen function and bacterial community in sheep","translated_title":"","metadata":{"abstract":"Oxalic acid (OA) is a secondary compound occurring in a wide range of plants consumed by ruminants, especially in saline lands or in arid and semi-arid regions. However, its impact on the rumen microbial community and its changes over time, as well as the potential consequences on ruminal function, remain unknown. To examine this impact, five ewes fitted with a ruminal cannula and fed low-quality grass hay were dosed daily with 0.6 mmol of OA/kg body weight through the cannula for 14 days. On days 0 (before the start), 4, 7 and 14 of the administration period, samples of ruminal digesta were collected throughout the day (0, 3, 6 and 9 h after the morning feeding) for analysis of the bacterial community and fermentation parameters (pH, ammonia and volatile fatty acid (VFA) concentrations). In addition, two feedstuffs were incubated in situ using the nylon bag technique to estimate ruminal degradation. Terminal restriction fragment length polymorphism was employed to monitor the dynamics of total bacteria, and quantitative real-time PCR was used to investigate the abundance of the oxalate-degrading Oxalobacter formigenes. Neither pH nor total VFA concentrations were affected. Nevertheless, OA dosing altered molar proportions of most individual VFA and ammonia concentrations (P \u0026amp;lt; 0.001). The dry matter disappearance of alfalfa hay was reduced on days 7 and 14 and that of barley straw only on day 7 (P \u0026amp;lt; 0.01). These slight changes were related to others observed in the relative frequency of a number of terminal restriction fragments. Variations in the ruminal microbiota occurred rapidly with OA administration, which did not modify the bacterial diversity significantly but altered the structure of the community. However, many of these changes were reversed by the end of the experiment, with no significant differences between days 0 and 14 of dosing. These results suggest a rapid adaptation of the rumen bacterial community linked to the estimated increase in the abundance of O. formigenes (from 0.002% to 0.007% of oxc gene in relation to the total bacteria 16S rDNA; P \u0026amp;lt; 0.01), which is assumed to be responsible for oxalate breakdown.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"animal"},"translated_abstract":"Oxalic acid (OA) is a secondary compound occurring in a wide range of plants consumed by ruminants, especially in saline lands or in arid and semi-arid regions. However, its impact on the rumen microbial community and its changes over time, as well as the potential consequences on ruminal function, remain unknown. To examine this impact, five ewes fitted with a ruminal cannula and fed low-quality grass hay were dosed daily with 0.6 mmol of OA/kg body weight through the cannula for 14 days. On days 0 (before the start), 4, 7 and 14 of the administration period, samples of ruminal digesta were collected throughout the day (0, 3, 6 and 9 h after the morning feeding) for analysis of the bacterial community and fermentation parameters (pH, ammonia and volatile fatty acid (VFA) concentrations). In addition, two feedstuffs were incubated in situ using the nylon bag technique to estimate ruminal degradation. Terminal restriction fragment length polymorphism was employed to monitor the dynamics of total bacteria, and quantitative real-time PCR was used to investigate the abundance of the oxalate-degrading Oxalobacter formigenes. Neither pH nor total VFA concentrations were affected. Nevertheless, OA dosing altered molar proportions of most individual VFA and ammonia concentrations (P \u0026amp;lt; 0.001). The dry matter disappearance of alfalfa hay was reduced on days 7 and 14 and that of barley straw only on day 7 (P \u0026amp;lt; 0.01). These slight changes were related to others observed in the relative frequency of a number of terminal restriction fragments. Variations in the ruminal microbiota occurred rapidly with OA administration, which did not modify the bacterial diversity significantly but altered the structure of the community. However, many of these changes were reversed by the end of the experiment, with no significant differences between days 0 and 14 of dosing. These results suggest a rapid adaptation of the rumen bacterial community linked to the estimated increase in the abundance of O. formigenes (from 0.002% to 0.007% of oxc gene in relation to the total bacteria 16S rDNA; P \u0026amp;lt; 0.01), which is assumed to be responsible for oxalate breakdown.","internal_url":"https://www.academia.edu/34129881/Impact_of_oxalic_acid_on_rumen_function_and_bacterial_community_in_sheep","translated_internal_url":"","created_at":"2017-08-04T04:20:28.762-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":66982274,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":29938383,"work_id":34129881,"tagging_user_id":66982274,"tagged_user_id":null,"co_author_invite_id":6465909,"email":"p***k@ole.com","display_order":0,"name":"P. 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The HT are antimicrobial, although some rumen bacteria can resist or degrade them into potentially toxic or harmless metabolites. To study the effect of the administration of HT-rich OL (Quercus pyrenaica) after a severe feed restriction on the rumen bacterial community and monitor the variations in some bacterial groups that are potentially able to resist or metabolize tannins, 3 ruminally cannulated bulls were initially fed grass hay and then subjected to a severe 8-day feed restriction period, before receiving OL for 6 days. Then, the animals were offered again grass hay for 12 more days. Rumen contents were sampled throughout the experiment. Quantitative real-time PCR and terminal restriction fragment length polymorphism (T-RFLP) were used to monitor the bacterial dynamics. Animal 1 was not intoxicated and showed lower relative abundances of Streptococcus bovis initially and after the OL administration than animals 2 and 3, which showed acute signs of intoxication. The genus Prevotella increased its abundance with the OL administration, whereas Selenomonas ruminantium was reduced. 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Hydrolysable tannins can undergo significant transformation in the rumen due to the presence ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... Hydrolysable tannins can undergo significant transformation in the rumen due to the presence of ester bonds that are cleaved by microbial enzymes, and there is sufficient evidence in the literature to support rumen microbial adaptation and degradation of those secondary ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34129878"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34129878"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34129878; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34129878]").text(description); $(".js-view-count[data-work-id=34129878]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34129878; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34129878']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34129878, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=34129878]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34129878,"title":"Effect of the administration of young oak (Quercus pyrenaica) leaves to cattle on ruminal fermentation","translated_title":"","metadata":{"abstract":"... 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Belenguer</a></span></div><div class="wp-workCard_item"><span>Journal of dairy science</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Rumen microbial biohydrogenation of dietary unsaturated fatty acids has a major effect on the pro...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Rumen microbial biohydrogenation of dietary unsaturated fatty acids has a major effect on the process of developing healthier dairy products. This study aimed to investigate in vivo the effect of diet supplementation with sunflower (SO) and fish (FO) oils on the rumen bacterial community in dairy sheep. First, 32 lactating ewes, divided in 8 lots of 4 animals each (2 lots per treatment), were fed a high-concentrate total mixed ration supplemented with 0, 2% SO, 1% FO, or 2% SO plus 1% FO. After 21 d, rumen fluid samples were taken from each lot for DNA extraction and fluorescence in situ hybridization (FISH) analysis. In a second experiment, 5 cannulated ewes were first fed the same TMR, with the exception of a higher forage level, and then changed to the same diet supplemented with 2% SO plus 1% FO. After 0, 3, and 10 d, rumen content samples were taken for DNA extraction and FISH analysis (fluid). 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