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Two pairs of 120and 180-day-old twin female lambs with a bodyweight of 18.2-19.0 kg were kept in metabolism crates and fed on purinefree milk replacement (MR) with supplements of exogenous purine (purine base or purine nucleoside) at a level of 0.2 mmol/BW 0.75 /d for 5 consecutive days, and thereafter they were maintained in the crates for 4 days. The daily amount of exogenous purine supply was calculated based on the urinary excreted purine derivatives (PD) in lambs fed on milk replacement alone. A urine sample was collected daily for 9 consecutive days, and the urinary excretion of PD was determined daily. Urinary PD excretion opened to increase within 24 h after the dose of purine bases, and the level was recovered on 3 days after ceasing the exogenous purine supply. The recovery of PD in the urine was about 70% of the purine supplement. When purine nucleosides were added to the feed, urinary PD excretion was initiated within 24 h after dosing, and the values were recovered after ending the purine nucleoside supply. The recovery rate of PD in the urine was only 30% of the supplemented purine. The plasma allantoin levels were almost similar after feeding purine bases and purine nucleosides, and the values were mostly in the range (40-60 µmol/L). These findings indicate that an exogenous purine can be directly incorporated into the body, and the purine as nucleoside is more effectively utilized for the synthesis of nucleic acids than as a purine base in the body of growing lambs.","publication_date":"2006,,","publication_name":"Animal Science Journal","grobid_abstract_attachment_id":"68956044"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"The effect of exogenous purine supply on the endogenous excretion of purine derivatives in the urine of growing lambs","broadcastable":false,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [13428366]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon'; 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if (!viewCountBody) { throw new Error('Failed to find work views element'); } viewCountBody.textContent = `${commaizedViewCount} views`; } catch (error) { // Remove the whole views element if there was some issue parsing. document.getElementById('work-metadata-view-count')?.parentNode?.remove(); throw new Error(`Failed to parse view count: ${viewCount}`, error); } }; // If the DOM is still loading, wait for it to be ready before updating the view count. if (document.readyState === "loading") { document.addEventListener('DOMContentLoaded', () => { updateViewCount(viewCount); }); // Otherwise, just update it immediately. } else { updateViewCount(viewCount); } })();</script></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">An experiment was made to determine the absorption of purine metabolites in dietary nucleic acids through the digestive tract, and also to determine the utilization of nucleic acids absorbed in the body, using growing lambs. Two pairs of 120and 180-day-old twin female lambs with a bodyweight of 18.2-19.0 kg were kept in metabolism crates and fed on purinefree milk replacement (MR) with supplements of exogenous purine (purine base or purine nucleoside) at a level of 0.2 mmol/BW 0.75 /d for 5 consecutive days, and thereafter they were maintained in the crates for 4 days. The daily amount of exogenous purine supply was calculated based on the urinary excreted purine derivatives (PD) in lambs fed on milk replacement alone. A urine sample was collected daily for 9 consecutive days, and the urinary excretion of PD was determined daily. Urinary PD excretion opened to increase within 24 h after the dose of purine bases, and the level was recovered on 3 days after ceasing the exogenous purine supply. The recovery of PD in the urine was about 70% of the purine supplement. When purine nucleosides were added to the feed, urinary PD excretion was initiated within 24 h after dosing, and the values were recovered after ending the purine nucleoside supply. The recovery rate of PD in the urine was only 30% of the supplemented purine. The plasma allantoin levels were almost similar after feeding purine bases and purine nucleosides, and the values were mostly in the range (40-60 µmol/L). These findings indicate that an exogenous purine can be directly incorporated into the body, and the purine as nucleoside is more effectively utilized for the synthesis of nucleic acids than as a purine base in the body of growing lambs.</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":68956044,"attachmentType":"pdf","workUrl":"https://www.academia.edu/51109462/The_effect_of_exogenous_purine_supply_on_the_endogenous_excretion_of_purine_derivatives_in_the_urine_of_growing_lambs"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":68956044,"attachmentType":"pdf","workUrl":"https://www.academia.edu/51109462/The_effect_of_exogenous_purine_supply_on_the_endogenous_excretion_of_purine_derivatives_in_the_urine_of_growing_lambs"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div><div class="ds-signup-banner-trigger-container"><div class="ds-signup-banner-trigger ds-signup-banner-trigger-control"></div></div><div class="ds-signup-banner ds-signup-banner-control"><div id="ds-signup-banner-close-button"><button class="ds2-5-button ds2-5-button--secondary ds2-5-button--inverse"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">close</span></button></div><div class="ds-signup-banner-ctas"><img src="//a.academia-assets.com/images/academia-logo-capital-white.svg" /><h4 class="ds2-5-heading-serif-sm">Sign up for access to the world's latest research</h4><button class="ds2-5-button ds2-5-button--inverse ds2-5-button--full-width js-swp-download-button" data-signup-modal="{"location":"signup-banner"}">Sign up for free<span class="material-symbols-outlined" style="font-size: 20px" translate="no">arrow_forward</span></button></div><div class="ds-signup-banner-divider"></div><div class="ds-signup-banner-reasons"><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Get notified about relevant papers</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Save papers to use in your research</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Join the discussion with peers</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Track your impact</span></div></div></div><script>(() => { // Set up signup banner show/hide behavior: // 1. 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For both experiments, 8 Nellore heifers fitted with ruminal and abomasal cannulas were allocated to two 4 × 4 Latin squares. The diets were based on corn silage and concentrate (60 and 40% DM basis, respectively); feces and urine samples were obtained by total collection, and abomasal DM flow was estimated using indigestible NDF as an internal marker. In Exp. I, 4 of the 8 heifers (BW 258 ± 20 kg) were also fitted with ileal cannula. The planned treatments were 4 different DMI: 1.2, 1.6, 2.0, and 2.4% of BW (DM basis). The endogenous losses and purine recovery as urinary PD were estimated using linear regression between daily urinary PD excretion (Y) and daily abomasal flow of purine bases (X), expressed in millimoles per kilogram of BW 0.75. In Exp. II, the same 8 Nellore heifers (BW of 296 ± 15 kg) were fed at 1.37% BW (DM basis). The treatments were the infusion of purines (RNA from torula yeast, type VI, Sigma) into the abomasum in increasing amounts (0, 33, 66, and 100 mmol/d). All statistical analyses were performed using the PROC MIXED procedure in SAS. In Exp. I, the DMI range was 1.16 to 1.84% of BW and did not affect (P > 0.05) the apparent RNA digestibility in the small intestine, which had a mean of 75.6%, and a true digestibility of 93.0%. The mean ratio of the N-RNA to the total-N in the ruminal bacteria was 0.137. The daily urinary PD excretion (Y, mmol/kg of BW 0.75) was a function of RNA flow in the abomasum (X, mmol/ kg of BW 0.75): Y = 0.860X + 0.460, where 0.860 and 0.460 were the PD recovery of purines and the endogenous fraction (in mmol/kg of BW 0.75), respectively. In Exp. II, the daily urinary PD excretion was a function of RNA flow in the abomasum: Y = 0.741X + 0.301, where 0.741 and 0.301 were the recovery of PD in urine of infused purines and the endogenous losses (in mmol/ kg of BW 0.75), respectively. In conclusion, our data suggest that in Nellore heifers the respective values of endogenous PD excretion (mmol/kg of BW 0.75), urinary recovery of the purines absorbed in the abomasum, and true digestibility of RNA in the small intestine were 0.30, 0.80, and 0.93.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Endogenous fraction and urinary recovery of purine derivatives obtained by different methods in Nellore cattle","attachmentId":73479612,"attachmentType":"pdf","work_url":"https://www.academia.edu/59683647/Endogenous_fraction_and_urinary_recovery_of_purine_derivatives_obtained_by_different_methods_in_Nellore_cattle","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/59683647/Endogenous_fraction_and_urinary_recovery_of_purine_derivatives_obtained_by_different_methods_in_Nellore_cattle"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="52771479" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/52771479/Nitrogen_and_purine_metabolism_in_preruminant_and_ruminant_goat_kids_given_increasing_amounts_of_ribonucleic_acids">Nitrogen and purine metabolism in preruminant and ruminant goat kids given increasing amounts of ribonucleic acids</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34722123" href="https://slu-se.academia.edu/JELindberg">J E Lindberg</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Animal Feed Science and Technology, 1991</p><p class="ds-related-work--abstract ds2-5-body-sm">Lindberg. I.E., 1991. Nitrogen and purine metabolism in p?cruminant and ruminanr goat kidsgiven increasing amounts of ribonuclexacids. ;lmm. Fk?dSc~. 7Pchnol.. 35: 213-226. In Experiment I. goat kids were bottle fed a liquid duct comaining 0, 1.25, X50.4.38 or 6.25 gyeast ribonuclcicacid (RNA) per kilogram metabolic live weight (W'"-'5). In Experiment 2. thegoat kids were lraincd to bottle feed from birth. They were then adapted to a normal bay and concentrate diet fed at 30 g kg-' W"' in addition to 600 g daily of a liquid diet containing 0.4.6. 9.6 or 13.8 g RNA. In both experimcnw, the urinary excretion oftolal mlrogen. allantoin nilrogen, uric acid nitrogen and hypoxmthine nitrogen increased s~gmticantly (P-co.01) with increasing amounts of RNA. The urinary excretion ofammonia nitrogen and crcatinine nitrogen were unaffected by RNA additions. The digestihilitles of organic matter and neutral detergent tihre were unaffected by RNA additions in Experiment 2. The proportions of purine derivatives recovered in the urine, when RNA intake was incrpascd, was of the same order and followed the same oattern in Exoerimcnts 1 and 2. The results indicate that thcrc is no adaplation mechanism to a maintained flow of microbial nucleic acids in goats</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Nitrogen and purine metabolism in preruminant and ruminant goat kids given increasing amounts of ribonucleic acids","attachmentId":69876158,"attachmentType":"pdf","work_url":"https://www.academia.edu/52771479/Nitrogen_and_purine_metabolism_in_preruminant_and_ruminant_goat_kids_given_increasing_amounts_of_ribonucleic_acids","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/52771479/Nitrogen_and_purine_metabolism_in_preruminant_and_ruminant_goat_kids_given_increasing_amounts_of_ribonucleic_acids"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="125126468" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/125126468/Urinary_excretion_of_purine_derivatives_inBos_indicus_Bos_tauruscrossbred_cattle">Urinary excretion of purine derivatives inBos indicus×Bos tauruscrossbred cattle</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="303349941" href="https://independent.academia.edu/BelenguerAlvaro">Alvaro Belenguer</a></div><p class="ds-related-work--metadata ds2-5-body-xs">British Journal of Nutrition, 2005</p><p class="ds-related-work--abstract ds2-5-body-sm">Four experiments were performed to study the kinetics of purine metabolism and urinary excretion in Zebu crossbred cattle. Fasting excretion was established in Expt 1, using eighteen maleBos indicus×Bos tauruscrossbred cattle (261 (se 9·1) kg body weight), six of each of the following genotypes: 5/8Bos indicus, 1/2Bos indicusand 3/8Bos indicus. No significant differences were observed among genotypes in fasting purine derivative excretion (277·3 (se 35·43) μmol/metabolic body weight). In a second experiment we measured the xanthine oxidase activity, which was higher in liver than in duodenal mucosa (0·64 and 0·06 (se 0·12) units/g wet tissue per min respectively;P&gt;0·05) being in plasma 0·60 (se 0·36) units/l per min. The kinetics of uric acid were measured by intravenous pulse dose of [1,3-15N]uric acid (Expt 3). The cumulative recovery of the isotope in urine was 82 (se 6·69) %, and uric acid plasma removal, pool size and mean retention time were 0·284 (se 0·051) per h, 5·45 (se...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives inBos indicus×Bos tauruscrossbred cattle","attachmentId":119225117,"attachmentType":"pdf","work_url":"https://www.academia.edu/125126468/Urinary_excretion_of_purine_derivatives_inBos_indicus_Bos_tauruscrossbred_cattle","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/125126468/Urinary_excretion_of_purine_derivatives_inBos_indicus_Bos_tauruscrossbred_cattle"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="17908221" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/17908221/Excretion_of_Purine_Derivatives_by_Holstein_Cows_Abomasally_Infused_with_Incremental_Amounts_of_Purines">Excretion of Purine Derivatives by Holstein Cows Abomasally Infused with Incremental Amounts of Purines</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37823538" href="https://independent.academia.edu/RonaldHatfield">Ronald Hatfield</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Dairy Science, 1997</p><p class="ds-related-work--abstract ds2-5-body-sm">Five multiparous, ruminally cannulated Holstein cows (two lactating and three dry) weighing (X +/- SD) 667 +/- 35 kg were used to study the effect of abomasal purine infusion on the excretion of purine derivatives. Cows were fed corn silage four times daily at 90% of ad libitum intake (X = 9.16 kg of dry matter/d). Purines were infused into the abomasum as brewer&amp;amp;amp;#39;s yeast suspensions in five incremental amounts (0 to 380 mmol/d) during five experimental periods according to a 5 x 5 Latin square design. Periods were 7 d; purine infusions were conducted during the last 4 d, and urine was collected during the last 3 d of each period. Ruminal purine outflow in all cows was measured during an experimental period immediately preceding and immediately following the five infusion periods and in each cow during the 0-mmol/d infusion period of the experiment. The relationship between total (milk plus urine) daily excretion of purine derivatives (allantoin plus uric acid) and total (abomasal infusion plus ruminal outflow) daily purine flow was quantified by linear regression analysis and was described by the relationship: Y = 0.856X + 103 (r2 = 0.93). The slope (0.856) indicated that 86% of purines that reached the omasum were excreted as purine derivatives. In the two lactating cows, urinary purine derivatives accounted for 98.4% of the total purine derivatives that were excreted. Ruminal flow of microbial CP can be estimated from the CP:purine ratio of ruminal microorganisms and the excretion of purine derivatives.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Excretion of Purine Derivatives by Holstein Cows Abomasally Infused with Incremental Amounts of Purines","attachmentId":42218473,"attachmentType":"pdf","work_url":"https://www.academia.edu/17908221/Excretion_of_Purine_Derivatives_by_Holstein_Cows_Abomasally_Infused_with_Incremental_Amounts_of_Purines","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17908221/Excretion_of_Purine_Derivatives_by_Holstein_Cows_Abomasally_Infused_with_Incremental_Amounts_of_Purines"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="118674175" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/118674175/Urinary_excretion_of_purine_derivatives_as_influenced_by_GFR_and_plasma_retention_of_purines_in_cattle_i_Bos_indicus_i_i_Bos_taurus_i_and_buffalo_i_Bubalus_bubalis_i_bulls">Urinary excretion of purine derivatives as influenced by GFR and plasma retention of purines in cattle (<i>Bos indicus</i> × <i>Bos taurus</i>) and buffalo (<i>Bubalus bubalis</i>) bulls</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="5319447" href="https://ivri.academia.edu/AshokKumarVerma">Ashok Kumar Verma</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Animal and Feed Sciences, 2013</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives as influenced by GFR and plasma retention of purines in cattle (\u003ci\u003eBos indicus\u003c/i\u003e × \u003ci\u003eBos taurus\u003c/i\u003e) and buffalo (\u003ci\u003eBubalus bubalis\u003c/i\u003e) bulls","attachmentId":114244095,"attachmentType":"pdf","work_url":"https://www.academia.edu/118674175/Urinary_excretion_of_purine_derivatives_as_influenced_by_GFR_and_plasma_retention_of_purines_in_cattle_i_Bos_indicus_i_i_Bos_taurus_i_and_buffalo_i_Bubalus_bubalis_i_bulls","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/118674175/Urinary_excretion_of_purine_derivatives_as_influenced_by_GFR_and_plasma_retention_of_purines_in_cattle_i_Bos_indicus_i_i_Bos_taurus_i_and_buffalo_i_Bubalus_bubalis_i_bulls"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="68069664" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/68069664/Urinary_excretion_of_purine_derivatives_in_dry_buffalo_and_Fresian_cows">Urinary excretion of purine derivatives in dry buffalo and Fresian cows</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32962053" href="https://unina.academia.edu/MonicaCutrignelli">Monica Cutrignelli</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Italian Journal of …, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion of purine derivatives in dry buffalo and Fresian cows","attachmentId":78679142,"attachmentType":"pdf","work_url":"https://www.academia.edu/68069664/Urinary_excretion_of_purine_derivatives_in_dry_buffalo_and_Fresian_cows","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/68069664/Urinary_excretion_of_purine_derivatives_in_dry_buffalo_and_Fresian_cows"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="89224479" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/89224479/Excretion_of_Endogenous_Purine_Derivatives_in_Male_and_Female_Garut_sheep">Excretion of Endogenous Purine Derivatives in Male and Female Garut sheep</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="59857726" href="https://independent.academia.edu/ChusnulHanim">Chusnul Hanim</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Advances in Biological Sciences Research, 2022</p><p class="ds-related-work--abstract ds2-5-body-sm">The aim of this study was to determine the value of endogenous purine derivatives (PD) excreted in the urine Garut sheep. The endogenous PD excretion of male and female Garut sheep was compared using an independent student ttest. In this experiment, six males and six females Garut sheep of similar age and body weight (10 months and 25 kg, respectively) were used. The sheep are fed ad libitum with elephant grass and bran pollard in a 60:40 ratio during the adaptation period. The feed then was gradually reduced, starting at 90%, 60%, and 30% from initial amount, and then the sheep were fasted. The sheep were fasted for five days before the urine collection period began. Urine samples will be measured for endogenous PD excretion. The results showed that the excretion of allantoin, xanthine-hypoxanthine, and uric acid in fasted male Garut sheep were 0.040±0.005, 0.004±0.001, 0.014±0.001 mmol/W 0.75 /day, respectively, while in fasted female Garut sheep were 0.035±0.005, 0.004±0.001, 0.013±0.001mmol/W 0.75 /day, respectively. Endogenous PD excretion in male Garut sheep was 0.057±0.006 mmol/W 0.75 /day, while it was 0.052±0.006 mmol/W 0.75 /day in female Garut sheep. Based on the endogenous PD data, we obtained modified equations, which were Y = 0.84X + (0.057W 0.75 e-0.25x) for male Garut sheep and Y = 0.84X + (0.052W 0.75 e-0.25x) for female Garut sheep. In conclusion, sex differences in Garut sheep had no effect on the excretion of endogenous PD, but endogenous PD in male Garut sheep tended to be higher than in females.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Excretion of Endogenous Purine Derivatives in Male and Female Garut sheep","attachmentId":93061954,"attachmentType":"pdf","work_url":"https://www.academia.edu/89224479/Excretion_of_Endogenous_Purine_Derivatives_in_Male_and_Female_Garut_sheep","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/89224479/Excretion_of_Endogenous_Purine_Derivatives_in_Male_and_Female_Garut_sheep"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="70043978" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/70043978/Effect_of_dietary_energy_levels_on_the_urinary_excretion_of_purine_derivatives_in_sheep">Effect of dietary energy levels on the urinary excretion of purine derivatives in sheep</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="13428366" href="https://independent.academia.edu/MartinShem">Martin Shem</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Animal Science Journal, 2005</p><p class="ds-related-work--abstract ds2-5-body-sm">The effect of dietary energy levels on the urinary excretion of purine derivatives (PD) was investigated in sheep fed hay and concentrate diets. In addition, the possibility of using PD concentrations in spot samples of urine as a useful tool for determining daily PD excretion was also examined. Three female lambs (mean bodyweight (BW) ± standard error, 31.4 ± 1.0 kg) were fed three diets consisting of timothy hay (20 g dry matter/kg BW per day) and rolled barley at three different energy levels (low-energy (LE), medium-energy (ME) and high-energy (HE) at 1.0-, 1.2-and 1.5-fold greater than the maintenance level of energy (450 KJ/BW 0.75 /day), respectively) in a 3 × 3 Latin square design. The dietary protein level was almost constant (380 mg nitrogen/BW 0.75 /day as digestible crude protein base) and was adjusted using soybean protein. Digestible organic matter intake (DOMI) increased (P < 0.05) with an increase in the dietary energy level and there was a difference (P < 0.05) in the organic matter digestibility between the LE and HE diets. For urinary PD excretion, the differences between the LE and ME diets and the HE diet were significant (P < 0.05), although there was no clear difference between the LE and ME diets. There was a positive correlation (r = 0.47, P < 0.05) between urinary PD excretion and DOMI. There was also a correlation (r = 0.59, P < 0.05) between the urinary PD level at 7-8 h after the morning feed and the daily total PD excretion, suggesting that the PD level in spot urine samples could be useful for estimating microbial protein synthesized in the rumen and/or digested in the lower gut.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Effect of dietary energy levels on the urinary excretion of purine derivatives in sheep","attachmentId":79923515,"attachmentType":"pdf","work_url":"https://www.academia.edu/70043978/Effect_of_dietary_energy_levels_on_the_urinary_excretion_of_purine_derivatives_in_sheep","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/70043978/Effect_of_dietary_energy_levels_on_the_urinary_excretion_of_purine_derivatives_in_sheep"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="108378899" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/108378899/Urinary_excretion_and_blood_plasma_allantoin_in_lambs_and_young_goats_starved_and_refed_with_a_purine_free_diet">Urinary excretion and blood plasma allantoin in lambs and young goats starved and refed with a purine-free diet</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="13428366" href="https://independent.academia.edu/MartinShem">Martin Shem</a></div><p class="ds-related-work--metadata ds2-5-body-xs">The Journal of Agricultural Science, 2003</p><p class="ds-related-work--abstract ds2-5-body-sm">An experiment was carried out to determine endogenous excretion of allantoin (AN) in lambs and kids fasted and fed on a purine-free diet (milk replacer: MR). After weaning (40–50 days after birth), the animals were reared by feeding on MR until they were 120 days old. At 8 months of age, they were fasted for 8 days, and then re-fed on MR for 13 days. After weaning, AN excretion gradually decreased almost to a constant level at 80–120 days after birth (mean 0·14 mmol/kgW0·75/day). In goat kids, there was variation but no definite trend in urinary AN excretion in the period 40–120 days (mean 0·35 mmol/kgW0·75/day). Urinary AN excretion was very low after fasting for 8 days, mean values (per kgW0·75/day) being 0·13 mmoles for lambs and 0·17 mmoles for goat kids. In lambs, urinary AN excretion did not increase following subsequent re-feeding of MR, and for goat kids the increase was small. Changes in plasma AN concentration after fasting and re-feeding showed a similar response to that ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Urinary excretion and blood plasma allantoin in lambs and young goats starved and refed with a purine-free diet","attachmentId":106779291,"attachmentType":"pdf","work_url":"https://www.academia.edu/108378899/Urinary_excretion_and_blood_plasma_allantoin_in_lambs_and_young_goats_starved_and_refed_with_a_purine_free_diet","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/108378899/Urinary_excretion_and_blood_plasma_allantoin_in_lambs_and_young_goats_starved_and_refed_with_a_purine_free_diet"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":68956044,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":68956044,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_68956044" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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class="ds-related-work--metadata ds2-5-body-xs">Animal Science, 1997</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Renal and salivary clearance of purine derivatives in sheep","attachmentId":105731233,"attachmentType":"pdf","work_url":"https://www.academia.edu/106574626/Renal_and_salivary_clearance_of_purine_derivatives_in_sheep","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/106574626/Renal_and_salivary_clearance_of_purine_derivatives_in_sheep"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="3" data-entity-id="55633082" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/55633082/Nitrogen_metabolism_and_urinary_excretion_of_purines_in_goat_kids">Nitrogen metabolism and urinary excretion of purines in goat kids</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="25884547" href="https://independent.academia.edu/JanErikLindberg">Jan Erik Lindberg</a></div><p class="ds-related-work--metadata ds2-5-body-xs">British Journal of Nutrition, 1989</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Nitrogen metabolism 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