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data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">7</p></div></a><a><div class="stat-container js-profile-coauthors" data-broccoli-component="user-info.coauthors-count" data-click-track="profile-expand-user-info-coauthors"><p class="label">Co-authors</p><p class="data">6</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div><div class="user-bio-container"><div class="profile-bio fake-truncate js-profile-about" style="margin: 0px;"><b>Address: </b>National Institute of Technology Karnataka, Surathkal, Mangalore, India 575025<br /><div class="js-profile-less-about u-linkUnstyled u-tcGrayDarker u-textDecorationUnderline u-displayNone">less</div></div></div><div class="ri-section"><div class="ri-section-header"><span>Interests</span></div><div class="ri-tags-container"><a data-click-track="profile-user-info-expand-research-interests" data-has-card-for-ri-list="33077347" href="https://www.academia.edu/Documents/in/Chemical_Engineering"><div id="js-react-on-rails-context" style="display:none" data-rails-context="{"inMailer":false,"i18nLocale":"en","i18nDefaultLocale":"en","href":"https://nitk.academia.edu/REGUPATHIIYYASWAMI","location":"/REGUPATHIIYYASWAMI","scheme":"https","host":"nitk.academia.edu","port":null,"pathname":"/REGUPATHIIYYASWAMI","search":null,"httpAcceptLanguage":null,"serverSide":false}"></div> <div class="js-react-on-rails-component" style="display:none" data-component-name="Pill" data-props="{"color":"gray","children":["Chemical Engineering"]}" data-trace="false" data-dom-id="Pill-react-component-f64147d3-b8b9-4e66-b440-9301d8af6cb8"></div> <div id="Pill-react-component-f64147d3-b8b9-4e66-b440-9301d8af6cb8"></div> </a></div></div><div class="external-links-container"><ul class="profile-links new-profile js-UserInfo-social"><li class="profile-profiles js-social-profiles-container"><i class="fa fa-spin fa-spinner"></i></li></ul></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by REGUPATHI IYYASWAMI</h3></div><div class="js-work-strip profile--work_container" data-work-id="87129494"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/87129494/Partitioning_Studies_of_Lactoperoxidase_from_Whey_Using_the_Reverse_Micelles_of_Non_Ionic_Ionic_Mixed_Surfactants"><img alt="Research paper thumbnail of Partitioning Studies of Lactoperoxidase from Whey Using the Reverse Micelles of Non-Ionic/Ionic Mixed Surfactants" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/87129494/Partitioning_Studies_of_Lactoperoxidase_from_Whey_Using_the_Reverse_Micelles_of_Non_Ionic_Ionic_Mixed_Surfactants">Partitioning Studies of Lactoperoxidase from Whey Using the Reverse Micelles of Non-Ionic/Ionic Mixed Surfactants</a></div><div class="wp-workCard_item"><span>SSRN Electronic Journal</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129494"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129494"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129494; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129494]").text(description); 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wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129489/Alcohol_based_aqueous_biphasic_system_applied_to_partition_four_different_natural_bioactive_compounds_from_Garcinia_indica_Choisy">Alcohol-based aqueous biphasic system applied to partition four different natural bioactive compounds from Garcinia indica Choisy</a></div><div class="wp-workCard_item"><span>Separation Science and Technology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric aci...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. The purity and recovery were further improved by employing the second stage ATPS.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129489"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129489"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129489; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129489]").text(description); $(".js-view-count[data-work-id=87129489]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87129489; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87129489']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87129489, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=87129489]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87129489,"title":"Alcohol-based aqueous biphasic system applied to partition four different natural bioactive compounds from Garcinia indica Choisy","translated_title":"","metadata":{"abstract":"ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. The purity and recovery were further improved by employing the second stage ATPS.","publisher":"Informa UK Limited","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Separation Science and Technology"},"translated_abstract":"ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87129483"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87129483/Analysis_of_ionic_and_nonionic_surfactants_blends_used_for_the_reverse_micellar_extraction_of_Lactoperoxidase_from_whey"><img alt="Research paper thumbnail of Analysis of ionic and nonionic surfactants blends used for the reverse micellar extraction of Lactoperoxidase from whey" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129483/Analysis_of_ionic_and_nonionic_surfactants_blends_used_for_the_reverse_micellar_extraction_of_Lactoperoxidase_from_whey">Analysis of ionic and nonionic surfactants blends used for the reverse micellar extraction of Lactoperoxidase from whey</a></div><div class="wp-workCard_item"><span>Asia-Pacific Journal of Chemical Engineering</span><span>, 2020</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129483"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129483"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129483; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87129479"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87129479/Partitioning_of_bio_active_compounds_from_rinds_of_garcinia_indica_using_aqueous_two_phase_system_Process_evaluation_and_optimization"><img alt="Research paper thumbnail of Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129479/Partitioning_of_bio_active_compounds_from_rinds_of_garcinia_indica_using_aqueous_two_phase_system_Process_evaluation_and_optimization">Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization</a></div><div class="wp-workCard_item"><span>Separation and Purification Technology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129479"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129479"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129479; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129479]").text(description); $(".js-view-count[data-work-id=87129479]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87129479; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87129479']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87129479, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=87129479]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87129479,"title":"Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization","translated_title":"","metadata":{"abstract":"Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87129457"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87129457/Comparison_of_antioxidant_properties_of_phenolic_compounds_and_their_effectiveness_in_imparting_oxidative_stability_to_sardine_oil_during_storage"><img alt="Research paper thumbnail of Comparison of antioxidant properties of phenolic compounds and their effectiveness in imparting oxidative stability to sardine oil during storage" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129457/Comparison_of_antioxidant_properties_of_phenolic_compounds_and_their_effectiveness_in_imparting_oxidative_stability_to_sardine_oil_during_storage">Comparison of antioxidant properties of phenolic compounds and their effectiveness in imparting oxidative stability to sardine oil during storage</a></div><div class="wp-workCard_item"><span>LWT - Food Science and Technology</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and fla...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and flavonoids) were chosen for the study with the aim to identify the ones which gives highest oxidative stability to the sardine oil. Initially, DPPH (2,2-diphenyl-1-picryl hydrazyl) scavenging activity, ferric reducing antioxidant power (FRAP) and metal chelating activity of all the antioxidants were determined through in vitro studies. Among all, quercetin showed the maximum DPPH activity, caffeic acid gave the maximum FRAP and catechin showed the highest metal chelating activity. Protective effect of all the phenolic compounds in stabilizing sardine oil were evaluated further, on the basis of peroxide values, p -anisidine value, conjugated dienes and thiobarbituric acid reactive substances during storage. Results showed that quercetin and rutin followed by caffeic acid were effective in restraining sardine oil oxidation. Ferulic acid and sinapic acid showed mild improvement in oxidative stability, whereas catechin had no effect.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129457"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129457"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129457; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129457]").text(description); $(".js-view-count[data-work-id=87129457]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87129457; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87129457']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87129457, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=87129457]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87129457,"title":"Comparison of antioxidant properties of phenolic compounds and their effectiveness in imparting oxidative stability to sardine oil during storage","translated_title":"","metadata":{"abstract":"Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and flavonoids) were chosen for the study with the aim to identify the ones which gives highest oxidative stability to the sardine oil. 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Ferulic acid and sinapic acid showed mild improvement in oxidative stability, whereas catechin had no effect.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"LWT - Food Science and Technology"},"translated_abstract":"Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and flavonoids) were chosen for the study with the aim to identify the ones which gives highest oxidative stability to the sardine oil. Initially, DPPH (2,2-diphenyl-1-picryl hydrazyl) scavenging activity, ferric reducing antioxidant power (FRAP) and metal chelating activity of all the antioxidants were determined through in vitro studies. Among all, quercetin showed the maximum DPPH activity, caffeic acid gave the maximum FRAP and catechin showed the highest metal chelating activity. 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The effect of rotor speed and phase flowrates on dispersed micellar phase holdup, mass transfer coefficient, yield, and purity were studied with micellar system contains TX114 (4.5 wt %)+ TMN6 (0.5 wt %) and 0.1 M ammonium chloride at broth pH of 3. The rotor speed and the micellar phase flowrate had a significant effect. A maximum PHA recovery of 85.48% and purity of 86.01% were achieved. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737770"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737770/Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system"><img alt="Research paper thumbnail of Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737770/Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system">Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system</a></div><div class="wp-workCard_item"><span>Preparative Biochemistry and Biotechnology</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research intere...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737770"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737770"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737770; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737770]").text(description); $(".js-view-count[data-work-id=77737770]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737770; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737770']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737770, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737770]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737770,"title":"Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system","translated_title":"","metadata":{"abstract":"Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.","publisher":"Informa UK Limited","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"Preparative Biochemistry and Biotechnology"},"translated_abstract":"Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.","internal_url":"https://www.academia.edu/77737770/Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system","translated_internal_url":"","created_at":"2022-04-26T22:12:20.211-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES"}],"urls":[{"id":19954226,"url":"https://www.tandfonline.com/doi/pdf/10.1080/10826068.2018.1504220"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737767"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737767/Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system"><img alt="Research paper thumbnail of Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737767/Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system">Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system</a></div><div class="wp-workCard_item"><span>Journal of Food Science and Technology</span><span>, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfact...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in -heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in -heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737767"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737767"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737767; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737767]").text(description); $(".js-view-count[data-work-id=77737767]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737767; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737767']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737767, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737767]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737767,"title":"Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system","translated_title":"","metadata":{"abstract":"The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in -heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in -heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent.","publisher":"Springer Nature","publication_date":{"day":null,"month":null,"year":2017,"errors":{}},"publication_name":"Journal of Food Science and Technology"},"translated_abstract":"The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in -heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in -heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent.","internal_url":"https://www.academia.edu/77737767/Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system","translated_internal_url":"","created_at":"2022-04-26T22:12:20.014-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":34478,"name":"Food Science and Technology","url":"https://www.academia.edu/Documents/in/Food_Science_and_Technology"},{"id":48321,"name":"Micelle","url":"https://www.academia.edu/Documents/in/Micelle"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences"},{"id":1380809,"name":"Pulmonary Surfactant","url":"https://www.academia.edu/Documents/in/Pulmonary_Surfactant"}],"urls":[{"id":19954225,"url":"http://link.springer.com/content/pdf/10.1007/s13197-017-2824-0.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737765"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737765/Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator"><img alt="Research paper thumbnail of Low frequency sonic waves assisted cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737765/Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator">Low frequency sonic waves assisted cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator</a></div><div class="wp-workCard_item"><span>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</span><span>, Jan 6, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of po...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay. In addition, sonic waves resulted in the separation of homogeneous surfactant and broth mixture into two distinct phases, top aqueous phase and polyhydroxyalkanoate enriched bottom surfactant rich phase. Mixed surfactant systems showed higher extraction efficiency compared to that of individual Triton X-100 concentrations, owing to increase in the hydrophobicity of the micellar core and its interaction with p...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737765"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737765"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737765; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737765]").text(description); $(".js-view-count[data-work-id=77737765]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737765; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737765']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737765, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737765]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737765,"title":"Low frequency sonic waves assisted cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator","translated_title":"","metadata":{"abstract":"Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay. In addition, sonic waves resulted in the separation of homogeneous surfactant and broth mixture into two distinct phases, top aqueous phase and polyhydroxyalkanoate enriched bottom surfactant rich phase. Mixed surfactant systems showed higher extraction efficiency compared to that of individual Triton X-100 concentrations, owing to increase in the hydrophobicity of the micellar core and its interaction with p...","publication_date":{"day":6,"month":1,"year":2017,"errors":{}},"publication_name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences"},"translated_abstract":"Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay. In addition, sonic waves resulted in the separation of homogeneous surfactant and broth mixture into two distinct phases, top aqueous phase and polyhydroxyalkanoate enriched bottom surfactant rich phase. Mixed surfactant systems showed higher extraction efficiency compared to that of individual Triton X-100 concentrations, owing to increase in the hydrophobicity of the micellar core and its interaction with p...","internal_url":"https://www.academia.edu/77737765/Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator","translated_internal_url":"","created_at":"2022-04-26T22:12:19.812-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":569587,"name":"Polyhydroxyalkanoates","url":"https://www.academia.edu/Documents/in/Polyhydroxyalkanoates"},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier"},{"id":960199,"name":"Sonication","url":"https://www.academia.edu/Documents/in/Sonication"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":3007910,"name":"Cupriavidus necator","url":"https://www.academia.edu/Documents/in/Cupriavidus_necator"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737763"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737763/Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii"><img alt="Research paper thumbnail of Anionic surfactant based reverse micellar extraction of L-asparaginase synthesized by Azotobacter vinelandii" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737763/Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii">Anionic surfactant based reverse micellar extraction of L-asparaginase synthesized by Azotobacter vinelandii</a></div><div class="wp-workCard_item"><span>Bioprocess and biosystems engineering</span><span>, Jan 6, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence o...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence of sucrose was successfully extracted using Reverse micellar extraction. Single step enzyme purification process was developed by varying the process variables which resulted in maximum specificity and extraction of L-asparaginase. The effect of different variables, including broth pH, addition of alcohol during the forward extraction and pH of the fresh stripping aqueous phase, addition of alcohol and electrolyte during backward extraction process were studied. Lower concentration of butanol resulted in maximum activity of the enzyme during forward extraction while enzyme activity was found to increase further with the addition of higher concentrations of ammonium sulphate during backward extraction. Chromatographic analysis of L-asparaginase peak at ~7.65 min was intense for the back extracted sample confirming the maximum purity of L-asparaginase obtained. Purity of L-asparaginase was ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737763"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737763"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737763; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737763]").text(description); $(".js-view-count[data-work-id=77737763]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737763; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737763']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737763, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737763]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737763,"title":"Anionic surfactant based reverse micellar extraction of L-asparaginase synthesized by Azotobacter vinelandii","translated_title":"","metadata":{"abstract":"L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence of sucrose was successfully extracted using Reverse micellar extraction. Single step enzyme purification process was developed by varying the process variables which resulted in maximum specificity and extraction of L-asparaginase. The effect of different variables, including broth pH, addition of alcohol during the forward extraction and pH of the fresh stripping aqueous phase, addition of alcohol and electrolyte during backward extraction process were studied. Lower concentration of butanol resulted in maximum activity of the enzyme during forward extraction while enzyme activity was found to increase further with the addition of higher concentrations of ammonium sulphate during backward extraction. Chromatographic analysis of L-asparaginase peak at ~7.65 min was intense for the back extracted sample confirming the maximum purity of L-asparaginase obtained. Purity of L-asparaginase was ...","publication_date":{"day":6,"month":1,"year":2017,"errors":{}},"publication_name":"Bioprocess and biosystems engineering"},"translated_abstract":"L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence of sucrose was successfully extracted using Reverse micellar extraction. Single step enzyme purification process was developed by varying the process variables which resulted in maximum specificity and extraction of L-asparaginase. The effect of different variables, including broth pH, addition of alcohol during the forward extraction and pH of the fresh stripping aqueous phase, addition of alcohol and electrolyte during backward extraction process were studied. Lower concentration of butanol resulted in maximum activity of the enzyme during forward extraction while enzyme activity was found to increase further with the addition of higher concentrations of ammonium sulphate during backward extraction. Chromatographic analysis of L-asparaginase peak at ~7.65 min was intense for the back extracted sample confirming the maximum purity of L-asparaginase obtained. Purity of L-asparaginase was ...","internal_url":"https://www.academia.edu/77737763/Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii","translated_internal_url":"","created_at":"2022-04-26T22:12:19.678-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography"},{"id":14934,"name":"Agricultural Biotechnology","url":"https://www.academia.edu/Documents/in/Agricultural_Biotechnology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":48321,"name":"Micelle","url":"https://www.academia.edu/Documents/in/Micelle"},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles"},{"id":564340,"name":"Surface Active Agents","url":"https://www.academia.edu/Documents/in/Surface_Active_Agents"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration"},{"id":1380809,"name":"Pulmonary Surfactant","url":"https://www.academia.edu/Documents/in/Pulmonary_Surfactant"},{"id":2491570,"name":"Azotobacter Vinelandii","url":"https://www.academia.edu/Documents/in/Azotobacter_Vinelandii"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737761"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737761/Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies"><img alt="Research paper thumbnail of Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737761/Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies">Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies</a></div><div class="wp-workCard_item"><span>Biotechnology and Biochemical Engineering</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737761"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737761"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737761; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737761]").text(description); $(".js-view-count[data-work-id=77737761]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737761; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737761']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737761, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737761]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737761,"title":"Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies","translated_title":"","metadata":{"abstract":"Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Biotechnology and Biochemical Engineering"},"translated_abstract":"Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.","internal_url":"https://www.academia.edu/77737761/Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies","translated_internal_url":"","created_at":"2022-04-26T22:12:19.550-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":273928,"name":"Biotechnology and Biochemical Engineering","url":"https://www.academia.edu/Documents/in/Biotechnology_and_Biochemical_Engineering"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737759"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737759/Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey"><img alt="Research paper thumbnail of Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737759/Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey">Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey</a></div><div class="wp-workCard_item"><span>Biotechnology and Biochemical Engineering</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the transferrin family, involves in the peroxidation reaction. Over the current decade, the enzyme has been emphasized for its properties such as antimicrobial, anti-inflammatory, anti-carcinogenic, etc. Bovine milk is considered to be the best source of milk proteins and Lactose peroxidase exist in trace amount among other milk proteins such as lactoferrin, lysozyme α-lactalbumin, β-lactoglobulin and Bovine serum albumin. Separation of specific low concentrated whey protein has been a difficult task owing to the difference in their composition in bovine milk and their similar physio-chemical properties. During partitioning of Lactose peroxidase, Lactoferrin competes as a result of similar physiochemical parameters making the separation process complex. Reverse micelle extraction has been employed to separate the proteins based on ionic interaction with charged surfactant head groups and has been effectively used in the separation of wide variety of biomolecules and chemophores. The present work elaborates an effective mixed reverse micellar extraction technique developed to separate Lactose peroxidase from whey. Critical micelle concentration of mixture of anionic and nonionic surfactant and a cationic and nonionic surfactant was studied. The surfactant systems at their critical micelle concentration was used for the extraction of Lactose peroxidase from whey. Process variables such as effect of pH, addition of alcohols and electrolytes on the extraction efficiency during forward and backward extraction of Lactose peroxidase from whey were examined.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737759"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737759"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737759; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737759]").text(description); $(".js-view-count[data-work-id=77737759]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737759; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737759']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737759, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737759]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737759,"title":"Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey","translated_title":"","metadata":{"abstract":"As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the transferrin family, involves in the peroxidation reaction. Over the current decade, the enzyme has been emphasized for its properties such as antimicrobial, anti-inflammatory, anti-carcinogenic, etc. Bovine milk is considered to be the best source of milk proteins and Lactose peroxidase exist in trace amount among other milk proteins such as lactoferrin, lysozyme α-lactalbumin, β-lactoglobulin and Bovine serum albumin. Separation of specific low concentrated whey protein has been a difficult task owing to the difference in their composition in bovine milk and their similar physio-chemical properties. During partitioning of Lactose peroxidase, Lactoferrin competes as a result of similar physiochemical parameters making the separation process complex. Reverse micelle extraction has been employed to separate the proteins based on ionic interaction with charged surfactant head groups and has been effectively used in the separation of wide variety of biomolecules and chemophores. The present work elaborates an effective mixed reverse micellar extraction technique developed to separate Lactose peroxidase from whey. Critical micelle concentration of mixture of anionic and nonionic surfactant and a cationic and nonionic surfactant was studied. The surfactant systems at their critical micelle concentration was used for the extraction of Lactose peroxidase from whey. Process variables such as effect of pH, addition of alcohols and electrolytes on the extraction efficiency during forward and backward extraction of Lactose peroxidase from whey were examined.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Biotechnology and Biochemical Engineering"},"translated_abstract":"As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the transferrin family, involves in the peroxidation reaction. Over the current decade, the enzyme has been emphasized for its properties such as antimicrobial, anti-inflammatory, anti-carcinogenic, etc. Bovine milk is considered to be the best source of milk proteins and Lactose peroxidase exist in trace amount among other milk proteins such as lactoferrin, lysozyme α-lactalbumin, β-lactoglobulin and Bovine serum albumin. Separation of specific low concentrated whey protein has been a difficult task owing to the difference in their composition in bovine milk and their similar physio-chemical properties. During partitioning of Lactose peroxidase, Lactoferrin competes as a result of similar physiochemical parameters making the separation process complex. Reverse micelle extraction has been employed to separate the proteins based on ionic interaction with charged surfactant head groups and has been effectively used in the separation of wide variety of biomolecules and chemophores. The present work elaborates an effective mixed reverse micellar extraction technique developed to separate Lactose peroxidase from whey. Critical micelle concentration of mixture of anionic and nonionic surfactant and a cationic and nonionic surfactant was studied. The surfactant systems at their critical micelle concentration was used for the extraction of Lactose peroxidase from whey. Process variables such as effect of pH, addition of alcohols and electrolytes on the extraction efficiency during forward and backward extraction of Lactose peroxidase from whey were examined.","internal_url":"https://www.academia.edu/77737759/Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey","translated_internal_url":"","created_at":"2022-04-26T22:12:19.419-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":273928,"name":"Biotechnology and Biochemical Engineering","url":"https://www.academia.edu/Documents/in/Biotechnology_and_Biochemical_Engineering"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737757"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737757/An_optimization_study_on_microwave_irradiated_decomposition_of_phenol_in_the_presence_of_H_2_O_2"><img alt="Research paper thumbnail of An optimization study on microwave irradiated decomposition of phenol in the presence of H 2 O 2" class="work-thumbnail" src="https://attachments.academia-assets.com/85025669/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737757/An_optimization_study_on_microwave_irradiated_decomposition_of_phenol_in_the_presence_of_H_2_O_2">An optimization study on microwave irradiated decomposition of phenol in the presence of H 2 O 2</a></div><div class="wp-workCard_item"><span>Journal of Chemical Technology & Biotechnology</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="079b8598e52aca44142a027b7894cd1b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":85025669,"asset_id":77737757,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/85025669/download_file?st=MTczMjQ2MzA2Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737757"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737757"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737757; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737757]").text(description); $(".js-view-count[data-work-id=77737757]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737757; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737757']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737757, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "079b8598e52aca44142a027b7894cd1b" } } $('.js-work-strip[data-work-id=77737757]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737757,"title":"An optimization study on microwave irradiated decomposition of phenol in the presence of H 2 O 2","translated_title":"","metadata":{"publisher":"Wiley-Blackwell","grobid_abstract":"BACKGROUND: Removal of phenol from industrial waste waters involves basic techniques namely extraction, biodegradation, photocatalytic degradation, etc. Among the available processes, the oxidation of phenols using H 2 O 2 is a suitable alternative because of low cost and high oxidizing power. The application of an oxidation process for the decomposition of stable organic compounds in waste water leads to the total degradation of the compounds rather than transferring from one form to another. Since oxidation using Fenton's reagent is more dependent on pH, in this present work it was proposed to use H 2 O 2 coupled with microwave irradiation. The effects of initial phenol concentration, microwave power and the irradiation time on the amount of decomposition were studied. RESULTS: In the present work experiments were conducted to estimate the percentage degradation of phenol for different initial concentrations of phenol (100, 200, 300, 400 and 500 mg L −1), microwave power input (180, 360, 540, 720 and 900 W) for different irradiation times. The kinetics of the degradation process were examined through experimental data and the decomposition rate follows first-order kinetics. Response surface methodology (RSM) was employed to optimize the design parameters for the present process. The interaction effect between the variables and the effect of interaction on to the responses (percentage decomposition of phenol) of the process was analysed and discussed in detail. The optimum values for the design parameters of the process were evaluated (initial phenol concentration 300 mg L −1 , microwave power output 668 W, and microwave irradiation time 60 s, giving phenol degradation 82.39%) through RSM by differential approximation, and were confirmed by experiment. CONCLUSION: The decomposition of phenol was carried out using H 2 O 2 coupled with microwave irradiation for different initial phenol concentrations, microwave power input and irradiation times. The phenol degradation process follows first-order kinetics. Optimization of the process was carried out through RSM by forming a design matrix using CCD. The optimized conditions were validated using experiments. 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extract in reassembled casein particles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/57052139/Selective_encapsulation_of_quercetin_from_dry_onion_peel_crude_extract_in_reassembled_casein_particles">Selective encapsulation of quercetin from dry onion peel crude extract in reassembled casein particles</a></div><div class="wp-workCard_item"><span>Food and Bioproducts Processing</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="57052139"><a 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data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/87129494/Partitioning_Studies_of_Lactoperoxidase_from_Whey_Using_the_Reverse_Micelles_of_Non_Ionic_Ionic_Mixed_Surfactants"><img alt="Research paper thumbnail of Partitioning Studies of Lactoperoxidase from Whey Using the Reverse Micelles of Non-Ionic/Ionic Mixed Surfactants" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/87129494/Partitioning_Studies_of_Lactoperoxidase_from_Whey_Using_the_Reverse_Micelles_of_Non_Ionic_Ionic_Mixed_Surfactants">Partitioning Studies of Lactoperoxidase from Whey Using the Reverse Micelles of Non-Ionic/Ionic Mixed Surfactants</a></div><div class="wp-workCard_item"><span>SSRN Electronic Journal</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129494"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129494"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129494; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129494]").text(description); 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wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129489/Alcohol_based_aqueous_biphasic_system_applied_to_partition_four_different_natural_bioactive_compounds_from_Garcinia_indica_Choisy">Alcohol-based aqueous biphasic system applied to partition four different natural bioactive compounds from Garcinia indica Choisy</a></div><div class="wp-workCard_item"><span>Separation Science and Technology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric aci...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. The purity and recovery were further improved by employing the second stage ATPS.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129489"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129489"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129489; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129489]").text(description); $(".js-view-count[data-work-id=87129489]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87129489; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87129489']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87129489, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=87129489]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87129489,"title":"Alcohol-based aqueous biphasic system applied to partition four different natural bioactive compounds from Garcinia indica Choisy","translated_title":"","metadata":{"abstract":"ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. The purity and recovery were further improved by employing the second stage ATPS.","publisher":"Informa UK Limited","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Separation Science and Technology"},"translated_abstract":"ABSTRACT An attempt was made to simultaneously partitioning Anthocyanins (ACN), Hydroxycitric acid (HCA), Garcinol (GL), and Isogarcinol (IGL) from fruit rinds of Garcinia indica using the 1-propanol and magnesium sulfate system. The influences of 1-propanol and magnesium sulfate concentration and Tie line Length (TLL) were investigated to obtain the optimal partitioning and recovery of GL and IGL into the top-phase and ACN and HCA to the bottom phase. The 25% w/w 1-propanol and 12% w/w magnesium sulfate system showed the maximum extraction efficiency for all the compounds. The purity and recovery were further improved by employing the second stage ATPS.","internal_url":"https://www.academia.edu/87129489/Alcohol_based_aqueous_biphasic_system_applied_to_partition_four_different_natural_bioactive_compounds_from_Garcinia_indica_Choisy","translated_internal_url":"","created_at":"2022-09-23T01:48:48.107-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Alcohol_based_aqueous_biphasic_system_applied_to_partition_four_different_natural_bioactive_compounds_from_Garcinia_indica_Choisy","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":55,"name":"Environmental Engineering","url":"https://www.academia.edu/Documents/in/Environmental_Engineering"},{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry"},{"id":989646,"name":"Aqueous Solution","url":"https://www.academia.edu/Documents/in/Aqueous_Solution"}],"urls":[{"id":24087806,"url":"https://www.tandfonline.com/doi/pdf/10.1080/01496395.2020.1802485"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87129483"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87129483/Analysis_of_ionic_and_nonionic_surfactants_blends_used_for_the_reverse_micellar_extraction_of_Lactoperoxidase_from_whey"><img alt="Research paper thumbnail of Analysis of ionic and nonionic surfactants blends used for the reverse micellar extraction of Lactoperoxidase from whey" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129483/Analysis_of_ionic_and_nonionic_surfactants_blends_used_for_the_reverse_micellar_extraction_of_Lactoperoxidase_from_whey">Analysis of ionic and nonionic surfactants blends used for the reverse micellar extraction of Lactoperoxidase from whey</a></div><div class="wp-workCard_item"><span>Asia-Pacific Journal of Chemical Engineering</span><span>, 2020</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129483"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129483"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129483; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87129479"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87129479/Partitioning_of_bio_active_compounds_from_rinds_of_garcinia_indica_using_aqueous_two_phase_system_Process_evaluation_and_optimization"><img alt="Research paper thumbnail of Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129479/Partitioning_of_bio_active_compounds_from_rinds_of_garcinia_indica_using_aqueous_two_phase_system_Process_evaluation_and_optimization">Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization</a></div><div class="wp-workCard_item"><span>Separation and Purification Technology</span><span>, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129479"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129479"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129479; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129479]").text(description); $(".js-view-count[data-work-id=87129479]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87129479; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87129479']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87129479, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=87129479]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87129479,"title":"Partitioning of bio-active compounds from rinds of garcinia indica using aqueous two-phase system: Process evaluation and optimization","translated_title":"","metadata":{"abstract":"Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2020,"errors":{}},"publication_name":"Separation and Purification Technology"},"translated_abstract":"Abstract The simultaneous extraction of anthocyanins (ACNs), garcinol (GL), isogarcinol (IGL) and hydroxycitric acid (HCA) from the rinds of Garcinia indica (GI) fruit and their partition behaviour in the aqueous two-phase system (ATPS) formed by 1-propanol-(NH4)2SO4 was investigated. The effect of different variables that govern the partition characteristics were studied and the amount of crude loaded into the ATPS, the concentration of 1-propanol and (NH4)2SO4 were found to be the significant variables. The Response Surface Methodology (RSM) was adopted to optimize the process variables through desirability based multi-response optimization for the partitioning of GL and IGL to the alcohol rich top phase and ACNs and HCA to the salt-rich bottom phase by considering the partitioning coefficients (K) and extraction efficiency of all the four bioactive components as responses. A ATPS consisting of 15.202% (w/w) 1-propanol, 10.242% (w/w) (NH4)2SO4 having the TLL of 28.505% (w/w) at a crude load of 25% (w/w) able to partition 97.39% GL (K = 370.770) and 92.38% IGL (K = 120.581) in the top phase and 99.19% ACNs (K = 0.080) and 99.83% HCA (K = 0.016) in the bottom phase with a purity higher than 99% by implementing secondary ATPS.","internal_url":"https://www.academia.edu/87129479/Partitioning_of_bio_active_compounds_from_rinds_of_garcinia_indica_using_aqueous_two_phase_system_Process_evaluation_and_optimization","translated_internal_url":"","created_at":"2022-09-23T01:48:39.148-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Partitioning_of_bio_active_compounds_from_rinds_of_garcinia_indica_using_aqueous_two_phase_system_Process_evaluation_and_optimization","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry"},{"id":989646,"name":"Aqueous Solution","url":"https://www.academia.edu/Documents/in/Aqueous_Solution"}],"urls":[{"id":24087800,"url":"https://api.elsevier.com/content/article/PII:S1383586620319948?httpAccept=text/xml"}]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="87129457"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/87129457/Comparison_of_antioxidant_properties_of_phenolic_compounds_and_their_effectiveness_in_imparting_oxidative_stability_to_sardine_oil_during_storage"><img alt="Research paper thumbnail of Comparison of antioxidant properties of phenolic compounds and their effectiveness in imparting oxidative stability to sardine oil during storage" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/87129457/Comparison_of_antioxidant_properties_of_phenolic_compounds_and_their_effectiveness_in_imparting_oxidative_stability_to_sardine_oil_during_storage">Comparison of antioxidant properties of phenolic compounds and their effectiveness in imparting oxidative stability to sardine oil during storage</a></div><div class="wp-workCard_item"><span>LWT - Food Science and Technology</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and fla...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and flavonoids) were chosen for the study with the aim to identify the ones which gives highest oxidative stability to the sardine oil. Initially, DPPH (2,2-diphenyl-1-picryl hydrazyl) scavenging activity, ferric reducing antioxidant power (FRAP) and metal chelating activity of all the antioxidants were determined through in vitro studies. Among all, quercetin showed the maximum DPPH activity, caffeic acid gave the maximum FRAP and catechin showed the highest metal chelating activity. Protective effect of all the phenolic compounds in stabilizing sardine oil were evaluated further, on the basis of peroxide values, p -anisidine value, conjugated dienes and thiobarbituric acid reactive substances during storage. Results showed that quercetin and rutin followed by caffeic acid were effective in restraining sardine oil oxidation. Ferulic acid and sinapic acid showed mild improvement in oxidative stability, whereas catechin had no effect.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="87129457"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="87129457"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 87129457; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=87129457]").text(description); $(".js-view-count[data-work-id=87129457]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 87129457; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='87129457']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 87129457, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=87129457]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":87129457,"title":"Comparison of antioxidant properties of phenolic compounds and their effectiveness in imparting oxidative stability to sardine oil during storage","translated_title":"","metadata":{"abstract":"Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and flavonoids) were chosen for the study with the aim to identify the ones which gives highest oxidative stability to the sardine oil. Initially, DPPH (2,2-diphenyl-1-picryl hydrazyl) scavenging activity, ferric reducing antioxidant power (FRAP) and metal chelating activity of all the antioxidants were determined through in vitro studies. Among all, quercetin showed the maximum DPPH activity, caffeic acid gave the maximum FRAP and catechin showed the highest metal chelating activity. Protective effect of all the phenolic compounds in stabilizing sardine oil were evaluated further, on the basis of peroxide values, p -anisidine value, conjugated dienes and thiobarbituric acid reactive substances during storage. Results showed that quercetin and rutin followed by caffeic acid were effective in restraining sardine oil oxidation. Ferulic acid and sinapic acid showed mild improvement in oxidative stability, whereas catechin had no effect.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"LWT - Food Science and Technology"},"translated_abstract":"Abstract Six phenolic compounds belonging to two different classes (hydroxycinnamic acids and flavonoids) were chosen for the study with the aim to identify the ones which gives highest oxidative stability to the sardine oil. Initially, DPPH (2,2-diphenyl-1-picryl hydrazyl) scavenging activity, ferric reducing antioxidant power (FRAP) and metal chelating activity of all the antioxidants were determined through in vitro studies. Among all, quercetin showed the maximum DPPH activity, caffeic acid gave the maximum FRAP and catechin showed the highest metal chelating activity. Protective effect of all the phenolic compounds in stabilizing sardine oil were evaluated further, on the basis of peroxide values, p -anisidine value, conjugated dienes and thiobarbituric acid reactive substances during storage. Results showed that quercetin and rutin followed by caffeic acid were effective in restraining sardine oil oxidation. 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The effect of rotor speed and phase flowrates on dispersed micellar phase holdup, mass transfer coefficient, yield, and purity were studied with micellar system contains TX114 (4.5 wt %)+ TMN6 (0.5 wt %) and 0.1 M ammonium chloride at broth pH of 3. The rotor speed and the micellar phase flowrate had a significant effect. A maximum PHA recovery of 85.48% and purity of 86.01% were achieved. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737770"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737770/Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system"><img alt="Research paper thumbnail of Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737770/Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system">Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system</a></div><div class="wp-workCard_item"><span>Preparative Biochemistry and Biotechnology</span><span>, 2018</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research intere...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737770"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737770"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737770; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737770]").text(description); $(".js-view-count[data-work-id=77737770]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737770; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737770']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737770, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737770]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737770,"title":"Aqueous two phase partitioning of Pisum sativum lectin in PEG/citrate salt system","translated_title":"","metadata":{"abstract":"Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.","publisher":"Informa UK Limited","publication_date":{"day":null,"month":null,"year":2018,"errors":{}},"publication_name":"Preparative Biochemistry and Biotechnology"},"translated_abstract":"Abstract Pisum sativum lectin (Psl) is a metalloprotein which is in the center of research interest because of its HIV-1 reverse transcriptase inhibitory activity and mitogenic activity. The application of this lectin in various fields demands the economically feasible and scalable purification strategy other than affinity chromatography. The suitability of aqueous two phase system (ATPS) composed of poly ethylene glycol (PEG) with different salts (sodium citrate, potassium citrate, and ammonium citrate) was evaluated for better partitioning of Psl. The significant factors such as molar mass and concentration of PEG, type and concentration of salts, the effect of tie line length (TLL), ionic strength, and pH were studied to select a suitable system for better partitioning of Psl. ATPS comprising of 18% PEG 6000, 16% sodium citrate, 1% NaCl at the operating condition of pH 8, 40.23% of TLL, and the volume ratio of 1.32 was found to be the best system which gave a maximum partition coefficient and yield of 14.5% and 98.66%, respectively.","internal_url":"https://www.academia.edu/77737770/Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system","translated_internal_url":"","created_at":"2022-04-26T22:12:20.211-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Aqueous_two_phase_partitioning_of_Pisum_sativum_lectin_in_PEG_citrate_salt_system","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES"}],"urls":[{"id":19954226,"url":"https://www.tandfonline.com/doi/pdf/10.1080/10826068.2018.1504220"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737767"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737767/Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system"><img alt="Research paper thumbnail of Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737767/Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system">Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system</a></div><div class="wp-workCard_item"><span>Journal of Food Science and Technology</span><span>, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfact...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in -heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in -heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737767"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737767"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737767; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737767]").text(description); $(".js-view-count[data-work-id=77737767]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737767; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737767']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737767, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737767]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737767,"title":"Reverse micellar extraction of lactoferrin from its synthetic solution using CTAB/n-heptanol system","translated_title":"","metadata":{"abstract":"The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in -heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in -heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent.","publisher":"Springer Nature","publication_date":{"day":null,"month":null,"year":2017,"errors":{}},"publication_name":"Journal of Food Science and Technology"},"translated_abstract":"The partitioning of Lactoferrin (LF) into the reverse micellar phase formed by a cationic surfactant, cetyltrimethylammonium bromide (CTAB) in -heptanol from the synthetic solution of LF was studied. The solubilization behaviour of LF into the reverse micellar phase and back extraction using a fresh stripping phase were improved by studying the effect of processing parameters, including surfactant concentration, solution pH, electrolyte salt concentration and addition of alcohol as co-solvent. Forward extraction of 100% was achieved at CTAB concentration of 50 mM in -heptanol solvent, pH of 10 and 1 M NaCl. The electrostatic force and hydrophobic interaction have major influence on LF extraction during forward and back extraction respectively. The size of the reverse micelles and their corresponding water content were measured at different operating conditions to assess their role on the LF extraction. The present reverse micellar system has potential to solubilise almost all the LF into the reverse micelles during forward extraction and could able to allow back extraction from the reverse micellar phase with addition of small amount of co-solvent.","internal_url":"https://www.academia.edu/77737767/Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system","translated_internal_url":"","created_at":"2022-04-26T22:12:20.014-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Reverse_micellar_extraction_of_lactoferrin_from_its_synthetic_solution_using_CTAB_n_heptanol_system","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":34478,"name":"Food Science and Technology","url":"https://www.academia.edu/Documents/in/Food_Science_and_Technology"},{"id":48321,"name":"Micelle","url":"https://www.academia.edu/Documents/in/Micelle"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences"},{"id":1380809,"name":"Pulmonary Surfactant","url":"https://www.academia.edu/Documents/in/Pulmonary_Surfactant"}],"urls":[{"id":19954225,"url":"http://link.springer.com/content/pdf/10.1007/s13197-017-2824-0.pdf"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737765"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737765/Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator"><img alt="Research paper thumbnail of Low frequency sonic waves assisted cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737765/Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator">Low frequency sonic waves assisted cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator</a></div><div class="wp-workCard_item"><span>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</span><span>, Jan 6, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of po...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay. In addition, sonic waves resulted in the separation of homogeneous surfactant and broth mixture into two distinct phases, top aqueous phase and polyhydroxyalkanoate enriched bottom surfactant rich phase. Mixed surfactant systems showed higher extraction efficiency compared to that of individual Triton X-100 concentrations, owing to increase in the hydrophobicity of the micellar core and its interaction with p...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737765"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737765"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737765; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737765]").text(description); $(".js-view-count[data-work-id=77737765]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737765; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737765']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737765, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737765]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737765,"title":"Low frequency sonic waves assisted cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator","translated_title":"","metadata":{"abstract":"Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay. In addition, sonic waves resulted in the separation of homogeneous surfactant and broth mixture into two distinct phases, top aqueous phase and polyhydroxyalkanoate enriched bottom surfactant rich phase. Mixed surfactant systems showed higher extraction efficiency compared to that of individual Triton X-100 concentrations, owing to increase in the hydrophobicity of the micellar core and its interaction with p...","publication_date":{"day":6,"month":1,"year":2017,"errors":{}},"publication_name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences"},"translated_abstract":"Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay. In addition, sonic waves resulted in the separation of homogeneous surfactant and broth mixture into two distinct phases, top aqueous phase and polyhydroxyalkanoate enriched bottom surfactant rich phase. Mixed surfactant systems showed higher extraction efficiency compared to that of individual Triton X-100 concentrations, owing to increase in the hydrophobicity of the micellar core and its interaction with p...","internal_url":"https://www.academia.edu/77737765/Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator","translated_internal_url":"","created_at":"2022-04-26T22:12:19.812-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Low_frequency_sonic_waves_assisted_cloud_point_extraction_of_polyhydroxyalkanoate_from_Cupriavidus_necator","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":569587,"name":"Polyhydroxyalkanoates","url":"https://www.academia.edu/Documents/in/Polyhydroxyalkanoates"},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier"},{"id":960199,"name":"Sonication","url":"https://www.academia.edu/Documents/in/Sonication"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":3007910,"name":"Cupriavidus necator","url":"https://www.academia.edu/Documents/in/Cupriavidus_necator"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737763"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737763/Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii"><img alt="Research paper thumbnail of Anionic surfactant based reverse micellar extraction of L-asparaginase synthesized by Azotobacter vinelandii" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737763/Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii">Anionic surfactant based reverse micellar extraction of L-asparaginase synthesized by Azotobacter vinelandii</a></div><div class="wp-workCard_item"><span>Bioprocess and biosystems engineering</span><span>, Jan 6, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence o...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence of sucrose was successfully extracted using Reverse micellar extraction. Single step enzyme purification process was developed by varying the process variables which resulted in maximum specificity and extraction of L-asparaginase. The effect of different variables, including broth pH, addition of alcohol during the forward extraction and pH of the fresh stripping aqueous phase, addition of alcohol and electrolyte during backward extraction process were studied. Lower concentration of butanol resulted in maximum activity of the enzyme during forward extraction while enzyme activity was found to increase further with the addition of higher concentrations of ammonium sulphate during backward extraction. Chromatographic analysis of L-asparaginase peak at ~7.65 min was intense for the back extracted sample confirming the maximum purity of L-asparaginase obtained. Purity of L-asparaginase was ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737763"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737763"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737763; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737763]").text(description); $(".js-view-count[data-work-id=77737763]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737763; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737763']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737763, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737763]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737763,"title":"Anionic surfactant based reverse micellar extraction of L-asparaginase synthesized by Azotobacter vinelandii","translated_title":"","metadata":{"abstract":"L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence of sucrose was successfully extracted using Reverse micellar extraction. Single step enzyme purification process was developed by varying the process variables which resulted in maximum specificity and extraction of L-asparaginase. The effect of different variables, including broth pH, addition of alcohol during the forward extraction and pH of the fresh stripping aqueous phase, addition of alcohol and electrolyte during backward extraction process were studied. Lower concentration of butanol resulted in maximum activity of the enzyme during forward extraction while enzyme activity was found to increase further with the addition of higher concentrations of ammonium sulphate during backward extraction. Chromatographic analysis of L-asparaginase peak at ~7.65 min was intense for the back extracted sample confirming the maximum purity of L-asparaginase obtained. Purity of L-asparaginase was ...","publication_date":{"day":6,"month":1,"year":2017,"errors":{}},"publication_name":"Bioprocess and biosystems engineering"},"translated_abstract":"L-Asparaginase synthesized by Azotobacter vinelandii via submerged fermentation in the presence of sucrose was successfully extracted using Reverse micellar extraction. Single step enzyme purification process was developed by varying the process variables which resulted in maximum specificity and extraction of L-asparaginase. The effect of different variables, including broth pH, addition of alcohol during the forward extraction and pH of the fresh stripping aqueous phase, addition of alcohol and electrolyte during backward extraction process were studied. Lower concentration of butanol resulted in maximum activity of the enzyme during forward extraction while enzyme activity was found to increase further with the addition of higher concentrations of ammonium sulphate during backward extraction. Chromatographic analysis of L-asparaginase peak at ~7.65 min was intense for the back extracted sample confirming the maximum purity of L-asparaginase obtained. Purity of L-asparaginase was ...","internal_url":"https://www.academia.edu/77737763/Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii","translated_internal_url":"","created_at":"2022-04-26T22:12:19.678-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Anionic_surfactant_based_reverse_micellar_extraction_of_L_asparaginase_synthesized_by_Azotobacter_vinelandii","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":72,"name":"Chemical Engineering","url":"https://www.academia.edu/Documents/in/Chemical_Engineering"},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering"},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography"},{"id":14934,"name":"Agricultural Biotechnology","url":"https://www.academia.edu/Documents/in/Agricultural_Biotechnology"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":48321,"name":"Micelle","url":"https://www.academia.edu/Documents/in/Micelle"},{"id":70047,"name":"Micelles","url":"https://www.academia.edu/Documents/in/Micelles"},{"id":564340,"name":"Surface Active Agents","url":"https://www.academia.edu/Documents/in/Surface_Active_Agents"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration"},{"id":1380809,"name":"Pulmonary Surfactant","url":"https://www.academia.edu/Documents/in/Pulmonary_Surfactant"},{"id":2491570,"name":"Azotobacter Vinelandii","url":"https://www.academia.edu/Documents/in/Azotobacter_Vinelandii"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737761"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737761/Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies"><img alt="Research paper thumbnail of Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737761/Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies">Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies</a></div><div class="wp-workCard_item"><span>Biotechnology and Biochemical Engineering</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737761"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737761"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737761; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737761]").text(description); $(".js-view-count[data-work-id=77737761]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737761; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737761']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737761, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737761]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737761,"title":"Aqueous Two-Phase (Acetonitrile–Potassium Citrate) Partitioning of Bovine Serum Albumin: Equilibrium and Application Studies","translated_title":"","metadata":{"abstract":"Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Biotechnology and Biochemical Engineering"},"translated_abstract":"Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery.","internal_url":"https://www.academia.edu/77737761/Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies","translated_internal_url":"","created_at":"2022-04-26T22:12:19.550-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Aqueous_Two_Phase_Acetonitrile_Potassium_Citrate_Partitioning_of_Bovine_Serum_Albumin_Equilibrium_and_Application_Studies","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":273928,"name":"Biotechnology and Biochemical Engineering","url":"https://www.academia.edu/Documents/in/Biotechnology_and_Biochemical_Engineering"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737759"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737759/Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey"><img alt="Research paper thumbnail of Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737759/Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey">Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey</a></div><div class="wp-workCard_item"><span>Biotechnology and Biochemical Engineering</span><span>, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the t...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the transferrin family, involves in the peroxidation reaction. Over the current decade, the enzyme has been emphasized for its properties such as antimicrobial, anti-inflammatory, anti-carcinogenic, etc. Bovine milk is considered to be the best source of milk proteins and Lactose peroxidase exist in trace amount among other milk proteins such as lactoferrin, lysozyme α-lactalbumin, β-lactoglobulin and Bovine serum albumin. Separation of specific low concentrated whey protein has been a difficult task owing to the difference in their composition in bovine milk and their similar physio-chemical properties. During partitioning of Lactose peroxidase, Lactoferrin competes as a result of similar physiochemical parameters making the separation process complex. Reverse micelle extraction has been employed to separate the proteins based on ionic interaction with charged surfactant head groups and has been effectively used in the separation of wide variety of biomolecules and chemophores. The present work elaborates an effective mixed reverse micellar extraction technique developed to separate Lactose peroxidase from whey. Critical micelle concentration of mixture of anionic and nonionic surfactant and a cationic and nonionic surfactant was studied. The surfactant systems at their critical micelle concentration was used for the extraction of Lactose peroxidase from whey. Process variables such as effect of pH, addition of alcohols and electrolytes on the extraction efficiency during forward and backward extraction of Lactose peroxidase from whey were examined.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737759"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737759"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737759; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737759]").text(description); $(".js-view-count[data-work-id=77737759]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737759; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737759']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737759, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=77737759]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737759,"title":"Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey","translated_title":"","metadata":{"abstract":"As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the transferrin family, involves in the peroxidation reaction. Over the current decade, the enzyme has been emphasized for its properties such as antimicrobial, anti-inflammatory, anti-carcinogenic, etc. Bovine milk is considered to be the best source of milk proteins and Lactose peroxidase exist in trace amount among other milk proteins such as lactoferrin, lysozyme α-lactalbumin, β-lactoglobulin and Bovine serum albumin. Separation of specific low concentrated whey protein has been a difficult task owing to the difference in their composition in bovine milk and their similar physio-chemical properties. During partitioning of Lactose peroxidase, Lactoferrin competes as a result of similar physiochemical parameters making the separation process complex. Reverse micelle extraction has been employed to separate the proteins based on ionic interaction with charged surfactant head groups and has been effectively used in the separation of wide variety of biomolecules and chemophores. The present work elaborates an effective mixed reverse micellar extraction technique developed to separate Lactose peroxidase from whey. Critical micelle concentration of mixture of anionic and nonionic surfactant and a cationic and nonionic surfactant was studied. The surfactant systems at their critical micelle concentration was used for the extraction of Lactose peroxidase from whey. Process variables such as effect of pH, addition of alcohols and electrolytes on the extraction efficiency during forward and backward extraction of Lactose peroxidase from whey were examined.","publication_date":{"day":null,"month":null,"year":2016,"errors":{}},"publication_name":"Biotechnology and Biochemical Engineering"},"translated_abstract":"As any other heme protein, Lactose peroxidase (LPO, E.C. 1.11.1.7) a enzyme that belongs to the transferrin family, involves in the peroxidation reaction. Over the current decade, the enzyme has been emphasized for its properties such as antimicrobial, anti-inflammatory, anti-carcinogenic, etc. Bovine milk is considered to be the best source of milk proteins and Lactose peroxidase exist in trace amount among other milk proteins such as lactoferrin, lysozyme α-lactalbumin, β-lactoglobulin and Bovine serum albumin. Separation of specific low concentrated whey protein has been a difficult task owing to the difference in their composition in bovine milk and their similar physio-chemical properties. During partitioning of Lactose peroxidase, Lactoferrin competes as a result of similar physiochemical parameters making the separation process complex. Reverse micelle extraction has been employed to separate the proteins based on ionic interaction with charged surfactant head groups and has been effectively used in the separation of wide variety of biomolecules and chemophores. The present work elaborates an effective mixed reverse micellar extraction technique developed to separate Lactose peroxidase from whey. Critical micelle concentration of mixture of anionic and nonionic surfactant and a cationic and nonionic surfactant was studied. The surfactant systems at their critical micelle concentration was used for the extraction of Lactose peroxidase from whey. Process variables such as effect of pH, addition of alcohols and electrolytes on the extraction efficiency during forward and backward extraction of Lactose peroxidase from whey were examined.","internal_url":"https://www.academia.edu/77737759/Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey","translated_internal_url":"","created_at":"2022-04-26T22:12:19.419-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":33077347,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Mixed_Surfactant_Based_Reverse_Micelle_Extraction_of_Lactose_Peroxidase_from_Whey","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":33077347,"first_name":"REGUPATHI","middle_initials":null,"last_name":"IYYASWAMI","page_name":"REGUPATHIIYYASWAMI","domain_name":"nitk","created_at":"2015-07-14T21:06:46.157-07:00","display_name":"REGUPATHI IYYASWAMI","url":"https://nitk.academia.edu/REGUPATHIIYYASWAMI"},"attachments":[],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry"},{"id":273928,"name":"Biotechnology and Biochemical Engineering","url":"https://www.academia.edu/Documents/in/Biotechnology_and_Biochemical_Engineering"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="77737757"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/77737757/An_optimization_study_on_microwave_irradiated_decomposition_of_phenol_in_the_presence_of_H_2_O_2"><img alt="Research paper thumbnail of An optimization study on microwave irradiated decomposition of phenol in the presence of H 2 O 2" class="work-thumbnail" src="https://attachments.academia-assets.com/85025669/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/77737757/An_optimization_study_on_microwave_irradiated_decomposition_of_phenol_in_the_presence_of_H_2_O_2">An optimization study on microwave irradiated decomposition of phenol in the presence of H 2 O 2</a></div><div class="wp-workCard_item"><span>Journal of Chemical Technology & Biotechnology</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="079b8598e52aca44142a027b7894cd1b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":85025669,"asset_id":77737757,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/85025669/download_file?st=MTczMjQ2MzA2Miw4LjIyMi4yMDguMTQ2&st=MTczMjQ2MzA2Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="77737757"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="77737757"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 77737757; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=77737757]").text(description); $(".js-view-count[data-work-id=77737757]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 77737757; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='77737757']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 77737757, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "079b8598e52aca44142a027b7894cd1b" } } $('.js-work-strip[data-work-id=77737757]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":77737757,"title":"An optimization study on microwave irradiated decomposition of phenol in the presence of H 2 O 2","translated_title":"","metadata":{"publisher":"Wiley-Blackwell","grobid_abstract":"BACKGROUND: Removal of phenol from industrial waste waters involves basic techniques namely extraction, biodegradation, photocatalytic degradation, etc. Among the available processes, the oxidation of phenols using H 2 O 2 is a suitable alternative because of low cost and high oxidizing power. The application of an oxidation process for the decomposition of stable organic compounds in waste water leads to the total degradation of the compounds rather than transferring from one form to another. Since oxidation using Fenton's reagent is more dependent on pH, in this present work it was proposed to use H 2 O 2 coupled with microwave irradiation. The effects of initial phenol concentration, microwave power and the irradiation time on the amount of decomposition were studied. RESULTS: In the present work experiments were conducted to estimate the percentage degradation of phenol for different initial concentrations of phenol (100, 200, 300, 400 and 500 mg L −1), microwave power input (180, 360, 540, 720 and 900 W) for different irradiation times. The kinetics of the degradation process were examined through experimental data and the decomposition rate follows first-order kinetics. Response surface methodology (RSM) was employed to optimize the design parameters for the present process. The interaction effect between the variables and the effect of interaction on to the responses (percentage decomposition of phenol) of the process was analysed and discussed in detail. The optimum values for the design parameters of the process were evaluated (initial phenol concentration 300 mg L −1 , microwave power output 668 W, and microwave irradiation time 60 s, giving phenol degradation 82.39%) through RSM by differential approximation, and were confirmed by experiment. CONCLUSION: The decomposition of phenol was carried out using H 2 O 2 coupled with microwave irradiation for different initial phenol concentrations, microwave power input and irradiation times. The phenol degradation process follows first-order kinetics. Optimization of the process was carried out through RSM by forming a design matrix using CCD. The optimized conditions were validated using experiments. 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