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phycocyanin</title> <meta name="description" content="Search results for: phycocyanin"> <meta name="keywords" content="phycocyanin"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="phycocyanin"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 8</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: phycocyanin</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Effect of Nutrient Limitations in Phycocyanin Formation by Spirulina platensis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hugo%20F.%20Lobaton">Hugo F. Lobaton</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The cyanobacterium Spirulina platensis is a prokaryotic photoautotrophic microorganism that is successfully cultivated for the commercialization as whole biomass due to its high protein content and promising valuable substance. For instance, phycocyanin has recently drawn the interest of the food and cosmetic industries due to its bright blue colour and its strong antioxidant capacities. The phycocyanin (PC) is the main protein-pigment in S. platensis (4% to 20%). In batches, the rate of overproduction of metabolites by cyanobacteria is limited or activated by the depletion of required substrates. The aim of this study was to develop a kinetic law that describes phycocyanin formation during batch cultivation. S. platensis was cultivated in 1 L bubble column photobioreactor with 30°C and 700 µmol m⁻² s⁻¹. Culture samples were daily collected from the bubble columns in sterile conditions. The biomass (g l⁻¹) was measured directly after a biomass lyophilisation process, and phycocyanin extractions and measurements were done according to a well-established protocol. A kinetic law for phycocyanin formation that includes nitrate and bicarbonate limitations was proposed and linked to the biomass core model. The set of differential equations were solved in MATLAB. Concerning to product formation, the experimental results show that phycocyanin mass fraction is degraded as results of the complete nitrate depletion and nitrate additions during the cultivation help to keep constant this molecule until new macro-element limitation appear. According to the model, bicarbonate is this limitation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phycocyanin" title="phycocyanin">phycocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrate" title=" nitrate"> nitrate</a>, <a href="https://publications.waset.org/abstracts/search?q=bicarbonate" title=" bicarbonate"> bicarbonate</a>, <a href="https://publications.waset.org/abstracts/search?q=spirulina" title=" spirulina"> spirulina</a> </p> <a href="https://publications.waset.org/abstracts/105879/effect-of-nutrient-limitations-in-phycocyanin-formation-by-spirulina-platensis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/105879.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">146</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Extraction of Phycocyanin from Spirulina platensis by Isoelectric Point Precipitation and Salting Out for Scale Up Processes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Velasco-Rend%C3%B3n%20Mar%C3%ADa%20Del%20Carmen">Velasco-Rendón María Del Carmen</a>, <a href="https://publications.waset.org/abstracts/search?q=Cu%C3%A9llar-Berm%C3%BAdez%20Sara%20Paulina"> Cuéllar-Bermúdez Sara Paulina</a>, <a href="https://publications.waset.org/abstracts/search?q=Parra-Sald%C3%ADvar%20Roberto"> Parra-Saldívar Roberto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Phycocyanin is a blue pigment protein with fluorescent activity produced by cyanobacteria. It has been recently studied to determine its anticancer, antioxidant and antiinflamatory potential. Since 2014 it was approved as a Generally Recognized As Safe (GRAS) proteic pigment for the food industry. Therefore, phycocyanin shows potential for the food, nutraceutical, pharmaceutical and diagnostics industry. Conventional phycocyanin extraction includes buffer solutions and ammonium sulphate followed by chromatography or ATPS for protein separation. Therefore, further purification steps are time-requiring, energy intensive and not suitable for scale-up processing. This work presents an alternative to conventional methods that also allows large scale application with commercially available equipment. The extraction was performed by exposing the dry biomass to mechanical cavitation and salting out with NaCl to use an edible reagent. Also, isoelectric point precipitation was used by addition of HCl and neutralization with NaOH. The results were measured and compared in phycocyanin concentration, purity and extraction yield. Results showed that the best extraction condition was the extraction by salting out with 0.20 M NaCl after 30 minutes cavitation, with a concentration in the supernatant of 2.22 mg/ml, a purity of 3.28 and recovery from crude extract of 81.27%. Mechanical cavitation presumably increased the solvent-biomass contact, making the crude extract visibly dark blue after centrifugation. Compared to other systems, our process has less purification steps, similar concentrations in the phycocyanin-rich fraction and higher purity. The contaminants present in our process edible NaCl or low pHs that can be neutralized. It also can be adapted to a semi-continuous process with commercially available equipment. This characteristics make this process an appealing alternative for phycocyanin extraction as a pigment for the food industry. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=extraction" title="extraction">extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=phycocyanin" title=" phycocyanin"> phycocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=precipitation" title=" precipitation"> precipitation</a>, <a href="https://publications.waset.org/abstracts/search?q=scale-up" title=" scale-up"> scale-up</a> </p> <a href="https://publications.waset.org/abstracts/27087/extraction-of-phycocyanin-from-spirulina-platensis-by-isoelectric-point-precipitation-and-salting-out-for-scale-up-processes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27087.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">438</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Antioxidant Effects of C-Phycocyanin on Oxidized Astrocyte in Brain Injury Using 2D and 3D Neural Nanofiber Tissue Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seung%20Ju%20Yeon">Seung Ju Yeon</a>, <a href="https://publications.waset.org/abstracts/search?q=Seul%20Ki%20Min"> Seul Ki Min</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Sang%20%20Park"> Jun Sang Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Yeo%20Seon%20Kwon"> Yeo Seon Kwon</a>, <a href="https://publications.waset.org/abstracts/search?q=Hoo%20Cheol%20Lee"> Hoo Cheol Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyun%20Jung%20Shim"> Hyun Jung Shim</a>, <a href="https://publications.waset.org/abstracts/search?q=Il-Doo%20Kim"> Il-Doo Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Ja%20Kyeong%20Lee"> Ja Kyeong Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Hwa%20Sung%20Shin"> Hwa Sung Shin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In brain injury, depleting oxidative stress is the most effective way to reduce the brain infarct size. C-phycocyanin (C-Pc) is a well-known antioxidant protein that has neuroprotective effects obtained from green microalgae. Astrocyte is glial cell that supports the nerve cell such as neuron, which account for a large portion of the brain. In brain injury, such as ischemia and reperfusion, astrocyte has an important rule that overcomes the oxidative stress and protect from brain reactive oxygen species (ROS) injury. However little is known about how C-Pc regulates the anti-oxidants effects of astrocyte. In this study, when the C-Pc was treated in oxidized astrocyte, we confirmed that inflammatory factors Interleukin-6 and Interleukin-3 were increased and antioxidants enzyme, Superoxide dismutase (SOD) and catalase was upregulated, and neurotrophic factors, brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) was alleviated. Also, it was confirmed to reduce infarct size of the brain in ischemia and reperfusion because C-Pc has anti-oxidant effects in middle cerebral artery occlusion (MCAO) animal model. These results show that C-Pc can help astrocytes lead neuroprotective activities in the oxidative stressed environment of the brain. In summary, the C-PC protects astrocytes from oxidative stress and has anti-oxidative, anti-inflammatory, neurotrophic effects under ischemic situations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=c-phycocyanin" title="c-phycocyanin">c-phycocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=astrocyte" title=" astrocyte"> astrocyte</a>, <a href="https://publications.waset.org/abstracts/search?q=reactive%20oxygen%20species" title=" reactive oxygen species"> reactive oxygen species</a>, <a href="https://publications.waset.org/abstracts/search?q=ischemia%20and%20reperfusion" title=" ischemia and reperfusion"> ischemia and reperfusion</a>, <a href="https://publications.waset.org/abstracts/search?q=neuroprotective%20effect" title=" neuroprotective effect"> neuroprotective effect</a> </p> <a href="https://publications.waset.org/abstracts/50872/antioxidant-effects-of-c-phycocyanin-on-oxidized-astrocyte-in-brain-injury-using-2d-and-3d-neural-nanofiber-tissue-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50872.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">320</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Isolation of Nitrosoguanidine Induced NaCl Tolerant Mutant of Spirulina platensis with Improved Growth and Phycocyanin Production</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Apurva%20Gupta">Apurva Gupta</a>, <a href="https://publications.waset.org/abstracts/search?q=Surendra%20Singh"> Surendra Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Spirulina spp., as a promising source of many commercially valuable products, is grown photo autotrophically in open ponds and raceways on a large scale. However, the economic exploitation in an open system seems to have been limited because of lack of multiple stress-tolerant strains. The present study aims to isolate a stable stress tolerant mutant of Spirulina platensis with improved growth rate and enhanced potential to produce its commercially valuable bioactive compounds. N-methyl-n'-nitro-n-nitrosoguanidine (NTG) at 250 μg/mL (concentration permitted 1% survival) was employed for chemical mutagenesis to generate random mutants and screened against NaCl. In a preliminary experiment, wild type S. platensis was treated with NaCl concentrations from 0.5-1.5 M to calculate its LC₅₀. Mutagenized colonies were then screened for tolerance at 0.8 M NaCl (LC₅₀), and the surviving colonies were designated as NaCl tolerant mutants of S. platensis. The mutant cells exhibited 1.5 times improved growth against NaCl stress as compared to the wild type strain in control conditions. This might be due to the ability of the mutant cells to protect its metabolic machinery against inhibitory effects of salt stress. Salt stress is known to adversely affect the rate of photosynthesis in cyanobacteria by causing degradation of the pigments. Interestingly, the mutant cells were able to protect its photosynthetic machinery and exhibited 4.23 and 1.72 times enhanced accumulation of Chl a and phycobiliproteins, respectively, which resulted in enhanced rate of photosynthesis (2.43 times) and respiration (1.38 times) against salt stress. Phycocyanin production in mutant cells was observed to enhance by 1.63 fold. Nitrogen metabolism plays a vital role in conferring halotolerance to cyanobacterial cells by influx of nitrate and efflux of Na+ ions from the cell. The NaCl tolerant mutant cells took up 2.29 times more nitrate as compared to the wild type and efficiently reduce it. Nitrate reductase and nitrite reductase activity in the mutant cells also improved by 2.45 and 2.31 times, respectively against salt stress. From these preliminary results, it could be deduced that enhanced nitrogen uptake and its efficient reduction might be a reason for adaptive and halotolerant behavior of the S. platensis mutant cells. Also, the NaCl tolerant mutant of S. platensis with significant improved growth and phycocyanin accumulation compared to the wild type can be commercially promising. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chemical%20mutagenesis" title="chemical mutagenesis">chemical mutagenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=NaCl%20tolerant%20mutant" title=" NaCl tolerant mutant"> NaCl tolerant mutant</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrogen%20metabolism" title=" nitrogen metabolism"> nitrogen metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=photosynthetic%20machinery" title=" photosynthetic machinery"> photosynthetic machinery</a>, <a href="https://publications.waset.org/abstracts/search?q=phycocyanin" title=" phycocyanin"> phycocyanin</a> </p> <a href="https://publications.waset.org/abstracts/78322/isolation-of-nitrosoguanidine-induced-nacl-tolerant-mutant-of-spirulina-platensis-with-improved-growth-and-phycocyanin-production" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78322.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">168</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Preparation of Magnetic Hydroxyapatite Composite by Wet Chemical Process for Phycobiliproteins Adsorption</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shu-Jen%20Chen">Shu-Jen Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Yi-Chien%20Wan"> Yi-Chien Wan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ruey-Chi%20Wang"> Ruey-Chi Wang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hydroxyapatite (Ca10(PO4)6(OH)2, HAp) can be applied to the fabrication of bone replacement materials, the composite of dental filling, and the adsorption of biomolecules and dyes. The integration of HAp and magnetic materials would offer several advantages for bio-separation process because the magnetic adsorbents is capable of recovered by applied magnetic field. C-phycocyanin (C-PC) and Allophycocyanin (APC), isolated from Spirulina platensis, can be used in fluorescent labeling probes, health care foods and clinical diagnostic reagents. Although the purification of C-PC and APC are reported by HAp adsorption, the adsorption of C-PC and APC by magnetic HAp composites was not reported yet. Therefore, the fabrication of HAp with magnetic silica nanoparticles for proteins adsorption was investigated in this work. First, the magnetic silica particles were prepared by covering silica layer on Fe3O4 nanoparticles with a reverse micelle method. Then, the Fe3O4@SiO2 nanoparticles were mixed with calcium carbonate to obtain magnetic silica/calcium carbonate composites (Fe3O4@SiO2/CaCO3). The Fe3O4@SiO2/CaCO3 was further reacted with K2HPO4 for preparing the magnetic silica/hydroxyapatite composites (Fe3O4@SiO2/HAp). The adsorption experiments indicated that the adsorption capacity of Fe3O4@SiO2/HAp toward C-PC and APC were highest at pH 6. The adsorption of C-PC and APC by Fe3O4@SiO2/HAp could be correlated by the pseudo-second-order model, indicating chemical adsorption dominating the adsorption process. Furthermore, the adsorption data showed that the adsorption of Fe3O4@SiO2/HAp toward C-PC and APC followed the Langmuir isotherm. The isoelectric points of C-PC and APC were around 5.0. Additionally, the zeta potential data showed the Fe3O4@SiO2/HAp composite was negative charged at pH 6. Accordingly, the adsorption mechanism of Fe3O4@SiO2/HAp toward C-PC and APC should be governed by hydrogen bonding rather than electrostatic interaction. On the other hand, as compared to C-PC, the Fe3O4@SiO2/HAp shows higher adsorption affinity toward APC. Although the Fe3O4@SiO2/HAp cannot recover C-PC and APC from Spirulina platensis homogenate, the Fe3O4@SiO2/HAp can be applied to separate C-PC and APC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hydroxyapatite" title="hydroxyapatite">hydroxyapatite</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic" title=" magnetic"> magnetic</a>, <a href="https://publications.waset.org/abstracts/search?q=C-phycocyanin" title=" C-phycocyanin"> C-phycocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=allophycocyanin" title=" allophycocyanin"> allophycocyanin</a> </p> <a href="https://publications.waset.org/abstracts/96630/preparation-of-magnetic-hydroxyapatite-composite-by-wet-chemical-process-for-phycobiliproteins-adsorption" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96630.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">152</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Protective Role of Phycobiliproteins in ROS-Associated Physiological Anomalies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ravi%20Raghav%20Sonani">Ravi Raghav Sonani</a>, <a href="https://publications.waset.org/abstracts/search?q=Niraj%20Kumar%20Singh"> Niraj Kumar Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Jitendra%20Kumar"> Jitendra Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Datta%20Madamwar"> Datta Madamwar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Phycobiliproteins (PBPs) are light harvesting proteins showing very strong absorbance and fluorescence in the visible range of the solar spectrum. Phycoerythrin (PE) and phycocyanin (PC) are majorly found PBPs in the cyanobacteria and red algae. In the present study, we have investigated the reactive oxygen species (ROS)-averting capacity of purified PE and PC of cyanobacterial origin. Furthermore, the possibility - whether the ROS-averting potential of PBPs can be explored in the therapeutics of oxidative stress associated physiological anomalies including aging and neurodegenerative diseases. The nematode Caenorhabditis elegans has been used as model organism in this study. PE and PC treatment moderated normal aging and associated physiological functionalities like pharyngeal pumping and locomotion of C. elegans. Moreover, PE-treatment enhanced the stress (oxidative and heat) tolerance upon PE and PC treatment. Specifically, PE treatment was also noted to moderate the progression of Alzheimer’s disease in transgenic C. elegans CL4176. However, PC-treatment curtailed the polyQ aggregation mediated proteotoxicity in C. elegans AM141 (Huntington disease model) under stressed (paraquat stress) as well as normal conditions. The effectiveness of PE and PC in expanding the lifespan of mutant C. elegans knockout for some up- (daf 16) and down- (daf-2 and age-1) stream regulators of insulin/IGF-1 signalling (IIS) shows the independency of their effects from DAF-2–AGE-1–DAF-16 signalling pathway. In conclusion, the present report demonstrates the anti-aging and neuro-protective potential of cyanobacterial PE and PC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phycobiliproteins" title="phycobiliproteins">phycobiliproteins</a>, <a href="https://publications.waset.org/abstracts/search?q=aging" title=" aging"> aging</a>, <a href="https://publications.waset.org/abstracts/search?q=alzheimer" title=" alzheimer"> alzheimer</a>, <a href="https://publications.waset.org/abstracts/search?q=huntington" title=" huntington"> huntington</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20elegans" title=" C. elegans"> C. elegans</a> </p> <a href="https://publications.waset.org/abstracts/45646/protective-role-of-phycobiliproteins-in-ros-associated-physiological-anomalies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45646.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">390</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Instrumental Characterization of Cyanobacteria as Polyhydroxybutyrate Producer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eva%20Slaninova">Eva Slaninova</a>, <a href="https://publications.waset.org/abstracts/search?q=Diana%20Cernayova"> Diana Cernayova</a>, <a href="https://publications.waset.org/abstracts/search?q=Zuzana%20Sedrlova"> Zuzana Sedrlova</a>, <a href="https://publications.waset.org/abstracts/search?q=Katerina%20Mrazova"> Katerina Mrazova</a>, <a href="https://publications.waset.org/abstracts/search?q=Petr%20Sedlacek"> Petr Sedlacek</a>, <a href="https://publications.waset.org/abstracts/search?q=Jana%20Nebesarova"> Jana Nebesarova</a>, <a href="https://publications.waset.org/abstracts/search?q=Stanislav%20Obruca"> Stanislav Obruca</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cyanobacteria are gram-negative prokaryotes belonging to a group of photosynthetic bacteria. In comparison with heterotrophic microorganisms, cyanobacteria utilize atmospheric nitrogen and carbon dioxide without any additional substrates. This ability of these microorganisms could be employed in biotechnology for the production of bioplastics, concretely polyhydroxyalkanoates (PHAs) which are primarily accumulated as a storage material in cells in the form of intracellular granules. In this study, there two cyanobacterial cultures from genera Synechocystis were used, namely Synechocystic sp. PCC 6803 and Synechocystis salina CCALA 192. There were optimized and used several various approaches, including microscopic techniques such as cryo-scanning electron microscopy (Cryo-SEM) and transmission electron microscopy (TEM), and fluorescence lifetime imaging microscopy using Nile red as a fluorescent probe (FLIM). Due to these instrumental techniques, the morphology of intracellular space and surface of cells were characterized. The next group of methods which were employed was spectroscopic techniques such as UV-Vis spectroscopy measured in two modes (turbidimetry and integration sphere) and Fourier transform infrared spectroscopy (FTIR). All these diverse techniques were used for the detection and characterization of pigments (chlorophylls, carotenoids, phycocyanin, etc.) and PHAs, in our case poly (3-hydroxybutyrate) (P3HB). To verify results, gas chromatography (GC) was employed concretely for the determination of the amount of P3HB in biomass. Cyanobacteria were also characterized as polyhydroxybutyrate producers by flow cytometer, which could count cells and at the same time distinguish cells including P3HB and without due to fluorescent probe called BODIPY and live/dead fluorescent probe SYTO Blue. Based on results, P3HB content in cyanobacteria cells was determined, as also the overall fitness of the cells. Acknowledgment: Funding: This study was partly funded by the projectGA19-29651L of the Czech Science Foundation (GACR) and partly funded by the Austrian Science Fund (FWF), project I 4082-B25. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cyanobacteria" title="cyanobacteria">cyanobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescent%20probe" title=" fluorescent probe"> fluorescent probe</a>, <a href="https://publications.waset.org/abstracts/search?q=microscopic%20techniques" title=" microscopic techniques"> microscopic techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=poly%283hydroxybutyrate%29" title=" poly(3hydroxybutyrate)"> poly(3hydroxybutyrate)</a>, <a href="https://publications.waset.org/abstracts/search?q=spectroscopy" title=" spectroscopy"> spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=chromatography" title=" chromatography"> chromatography</a> </p> <a href="https://publications.waset.org/abstracts/139809/instrumental-characterization-of-cyanobacteria-as-polyhydroxybutyrate-producer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139809.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">229</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Effects of Two Distinct Monsoon Seasons on the Water Quality of a Tropical Crater Lake</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maurice%20A.%20Duka">Maurice A. Duka</a>, <a href="https://publications.waset.org/abstracts/search?q=Leobel%20Von%20Q.%20Tamayo"> Leobel Von Q. Tamayo</a>, <a href="https://publications.waset.org/abstracts/search?q=Ni%C3%B1o%20Carlo%20I.%20Casim"> Niño Carlo I. Casim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The paucity of long-term measurements and monitoring of accurate water quality parameter profiles is evident for small and deep tropical lakes in Southeast Asia. This leads to a poor understanding of the stratification and mixing dynamics of these lakes in the region. The water quality dynamics of Sampaloc Lake, a tropical crater lake (104 ha, 27 m deep) in the Philippines, were investigated to understand how monsoon-driven conditions impact water quality and ecological health. Located in an urban area with approximately 10% of its surface area allocated to aquaculture, the lake is subject to distinct seasonal changes associated with the Northeast (NE) and Southwest (SW) monsoons. NE Monsoon typically occurs from October to April, while SW monsoon from May to September. These monsoons influence the lake’s water temperature, dissolved oxygen (DO), chlorophyll-α (chl-α), phycocyanin (PC), and turbidity, leading to significant seasonal variability. Monthly field observations of water quality parameters were made from October 2022 to September 2023 using a multi-parameter probe, YSI ProDSS, together with the collection of meteorological data during the same period. During the NE monsoon, cooler air temperatures and winds with sustained speeds caused surface water temperatures to drop from 30.9 ºC in October to 25.5 ºC in January, resulting in the weakening of stratification and eventually in lake turnover. This turnover redistributed nutrients from hypolimnetic layers to surface layers, increasing chl-α and PC levels (14-41 and 0-2 µg/L) throughout the water column. The fish kill was also observed during the lake’s turnover event as a result of the mixing of hypoxic hypolimnetic waters. Turbidity levels (0-3 NTU) were generally low but showed mid-column peaks in October, which was linked to thermocline-related effects, while low values in November followed heavy rainfall dilution and mixing effects. Conversely, the SW monsoon showed increased surface temperatures (28-30 ºC), shallow thermocline formations (3-11 m), and lower surface chl-α and PC levels (2-8 and 0-0.5 µg/L, respectively), likely due to limited nutrient mixing and more stable stratification. Turbidity was notably higher also in July (11-15 NTU) due to intense rainfall and reduced light penetration, which minimized photosynthetic activity. The SW monsoon also coincided with the typhoon season in the study area, resulting in partial upwelling of nutrients during strong storm events. These findings emphasize the need for continued monitoring of Sampaloc Lake’s seasonal water quality patterns, as monsoon-driven changes are crucial to maintaining its ecological balance and sustainability. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=seasonal%20water%20quality%20dynamics" title="seasonal water quality dynamics">seasonal water quality dynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=Philippine%20tropical%20lake" title=" Philippine tropical lake"> Philippine tropical lake</a>, <a href="https://publications.waset.org/abstracts/search?q=monsoon-driven%20conditions" title=" monsoon-driven conditions"> monsoon-driven conditions</a>, <a href="https://publications.waset.org/abstracts/search?q=stratification%20and%20mixing" title=" stratification and mixing"> stratification and mixing</a> </p> <a href="https://publications.waset.org/abstracts/193436/effects-of-two-distinct-monsoon-seasons-on-the-water-quality-of-a-tropical-crater-lake" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193436.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">10</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> 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