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Spatial Biology with RNAscope | Bio-Techne
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class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#see-more">See More With RNAscope™</a> | <a href="#products-applications">Products and Applications</a> | <a href="#health-and-disease">Spatial Insights in Health and Disease</a> | <a href="#therapeutics-development">Therapeutics Development</a> | <a href="#testimonials">Customer Testimonials</a> </p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/rnascope-spatial-biology-multiomics.jpg" alt="RNAscope multiomics on COMET spatial biology image" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h2><strong>Delivering Spatial Insights to Improve Lives</strong></h2><p> </p><p>Bio-Techne is a global leader in spatial biology, delivering powerful multiomic solutions to reveal ground-breaking discoveries, accelerate the development of next-generation therapeutics, and deliver life changing diagnostics. Reveal new insights in cell phenotype and function with confidence using gold-standard RNAscope™ technology, proven Bio-Techne antibodies, and the fully automated Lunaphore COMET™ system.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><a name="see-more"></a></p><h2>See More With RNAscope™</h2><h3>Many Spatial Questions. One Clear Answer. </h3><ul><li><p lang="EN-US">Accurately detect RNA expression at single-molecule sensitivity and subcellular resolution.</p></li><li><p lang="EN-US">Visualize and quantify any RNA. Select from mRNAs, ncRNAs, splice variants, reporters, transgenes and more.</p></li><li><p lang="EN-US">Easily detect RNA and protein biomarkers on the same section for a comprehensive view of single-cell phenotype and function.</p></li><li><p lang="EN-US">Characterize any organism or tissue with proven protocols.</p></li></ul><p> </p><p> </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/reagents/rnascope-ish-technology" class="btn btn-primary" type="link"> Learn More </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <a href="https://www.bio-techne.com/reagents/rnascope-ish-technology" title="Learn More " > <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/v2_acd-transition-graphic.png" alt="Spatial Biology Hub RNAscope Infographic" loading="lazy" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Search RNAscope Target and Control Probes</h2></div> <a href="/search?search_type=products&keywords=RNAscope%20probes" > <button type="button" class="btn btn-primary"> Search Probes </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="products-applications"></a></p><p> </p><h2><strong>Featured Spatial Biology Products and Applications</strong></h2></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/applications/spatial-biology/rnascope-multiomics" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/rnascope-colon-inflamation-image-gallery-image1-v2.jpg" alt="Detection of cytokines, immune and tumor cells using an RNAscope RNA-protein multiomic panel of 4 marker proteins, CD4 (yellow), CD8 (white), PanCK (pink), FoxP3 (green), and 2 target RNA probes, IFNG (red), GZMB (turquoise) in human colon cancer tissue" loading="lazy" > </div> </a> <a class="card_title_link" href="/applications/spatial-biology/rnascope-multiomics" > <h3 class="card_title_htag"> <div><h3>Spatial Multiomics</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Visualize cell phenotype and function with protease-free, same-slide codetection of protein and RNA.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/applications/spatial-biology/rnascope-multiomics" > Learn More </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://lunaphore.com/products/comet/" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/comet-instrument-480x480.png" alt="Comet Instrument 480x480" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://lunaphore.com/products/comet/" > <h3 class="card_title_htag"> <div>Lunaphore COMET™</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Unlock the potential of spatial biology with unmatched hyperplex throughput, walk-away automation, and rapid, flexible panel design.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://lunaphore.com/products/comet/" > Discover COMET at Lunaphore.com </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/applications/spatial-biology/rnascope-spatial-transcriptomics" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/rnascope-multiomics-sfn-panel1-brainc-pos-v3.jpg" alt="Profiling different cell types within the brain tumor microenvironment. Simultaneous detection of a panel of 3 marker proteins (CD8a, CD68, S100β) and 3 target RNA probes (CLDN5, CXCL10, TNFα) in brain tumor tissue." loading="lazy" > </div> </a> <a class="card_title_link" href="/applications/spatial-biology/rnascope-spatial-transcriptomics" > <h3 class="card_title_htag"> <div><h3>Single Cell Validation </h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Visualize the spatial localization and activation states of novel cell subtypes.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/applications/spatial-biology/rnascope-spatial-transcriptomics" > See How </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Select your IHC Antibodies for Spatial Biology</h2></div> <a href="/applications/imaging/immunohistochemistry" > <button type="button" class="btn btn-primary"> Find Your Antibody </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="health-and-disease"></a></p><p> </p><h2><strong>Reveal New Spatial Biology Insights in Health and Disease</strong></h2></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/applications/spatial-biology/rnascope-neuroscience" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/brain-light-blue-icon.png" alt="Representation of a brain" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/applications/spatial-biology/rnascope-neuroscience" > <h3 class="card_title_htag"> <div><h3>Neuroscience </h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Spatially interrogate neuronal cell subtypes, central inflammatory responses, and networks of pain and addiction.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/applications/spatial-biology/rnascope-neuroscience" > Explore More </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/applications/spatial-biology/rnascope-cancer" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/tumor-microenvironment-light-blue.png" alt="Representation of a tumor " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/applications/spatial-biology/rnascope-cancer" > <h3 class="card_title_htag"> <div><h3>Cancer </h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Uncover mechanisms underlying cancer initiation, progression, and therapeutic resistance.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/applications/spatial-biology/rnascope-cancer" > Learn More </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/applications/spatial-biology/rnascope-inflammation" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/immunology-icon-light-blue.png" alt="Representation of an immune cell" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/applications/spatial-biology/rnascope-inflammation" > <h3 class="card_title_htag"> <div><h3>Inflammation </h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Quantify and characterize immune cell infiltration and activation including cytokine chemokine expression.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/applications/spatial-biology/rnascope-inflammation" > See How </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/banners/blue-purple-gradient-1110x340.png" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/literature-icon-dark-blue.png" alt="Literature icon" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>Over 10K Spatial Biology Publications and Counting</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Easily find peer-reviewed research articles for your gene or research area of interest. See how RNAscope can help you uncover new spatial interests to achieve your research goals.</p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="/resources/rnascope-citations" class="btn btn-primary" type="link"> Search Publications </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="therapeutics-development"></a></p><h2> </h2><h2><strong>Accelerate Therapeutics Development</strong></h2></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/rnascope-ish-technology/gene-therapy" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/vaccine-development-icon-light-blue.png" alt="Vaccine development icon " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/reagents/rnascope-ish-technology/gene-therapy" > <h3 class="card_title_htag"> <div><h3>Gene Therapy</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Visualize AAV vector and ASO/siRNA biodistribution, cellular tropism, and transduction efficiency in spatial context.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/rnascope-ish-technology/gene-therapy" > Learn More </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/rnascope-ish-technology/cell-therapy" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/cell-gene-therapy-icon-light.png" alt="Representation of DNA being introduced into a cell " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/reagents/rnascope-ish-technology/cell-therapy" > <h3 class="card_title_htag"> <div><h3>Cell Therapy </h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Quantify infiltration and cellular activation of engineered cells in cancer and diseased tissues.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/rnascope-ish-technology/cell-therapy" > Learn More </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="testimonials"></a></p></div> </div> </div> <div> <div class="testimonial"> <div > <div class="row"> <div class="col-md-4"><!-- start left column --> <div class="left-title"> <h2>Customer Success Stories</h2> </div> </div><!-- end left column --> <div class="col-md-8"><!-- start right column --> <div class="right-card bg-white border rounded p-5 mb-4"> <div class="carousel slide" id="carouselIndicators" data-ride="carousel"> <div class="row"> <div class="col-md-12"> <div class="carousel-inner"> <div class="carousel-item active"> <!-- ...Continued from paragraph--carousel-testimonial --> <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-7e746eb3aa1" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/komal-headshot.png' alt='Dr. Ruchi Komal Ph.D. Headshot'> </div> </div> </div> </div> </div><!-- end column --> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Ruchi Komal PhD, Research Fellow </span><br/> <span class="individual-testimonial__position"> SLCR, National Institute of Mental Health, NIH </span><br/> </div> </div> </div><!-- end column --> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div><!-- end column --> </div><!-- end row --> </div><!-- end testimonial-info-group --> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> RNAscope spatial analysis greatly improves our research by offering high-resolution visualization of gene expression within the SCN... validating discoveries from single-cell data, identifying rare cell populations, and facilitating gene co-expression analysis in situ. </div> </div> </div> </div> </div> <div class="carousel-item"> <!-- ...Continued from paragraph--carousel-testimonial --> <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-fe9783b881e" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/authors-testimonials.png' alt='Author Testimonial Icon'> </div> </div> </div> </div> </div><!-- end column --> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Dr Denis Schapiro </span><br/> <span class="individual-testimonial__position"> Heidelberg University Hospital </span><br/> </div> </div> </div><!-- end column --> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div><!-- end column --> </div><!-- end row --> </div><!-- end testimonial-info-group --> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> The Lunaphore COMET™ system has enabled us to perform highly-multiplexed imaging of entire cross sections of mouse hearts with sub-cellular resolution. The flexible panel design accelerated antibody validation and measurements so that we can already perform spatial analysis of the heart at scale. </div> </div> </div> </div> </div> <div class="carousel-item"> <!-- ...Continued from paragraph--carousel-testimonial --> <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-fe9783b881e" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/authors-testimonials.png' alt='Author Testimonial Icon'> </div> </div> </div> </div> </div><!-- end column --> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Dr Cheryl Stoddart, Ekaterina Maidji </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div><!-- end column --> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div><!-- end column --> </div><!-- end row --> </div><!-- end testimonial-info-group --> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> The identification and anatomic location of HIV-infected cells and the effects of tissue environment on virus replication and viral gene expression are all critical for evaluating the pathogenesis of HIV infection and for developing cure strategies to eliminate HIV reservoirs. RNAscope in situ hybridization (ISH) has enabled us to visualize and quantify HIV RNA with single-copy resolution. 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