Milo Antibody Database

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class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> Resources </li> <li> Milo Antibody Database </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div id="simple_western_antibody_database_banner"> <h1>Milo Antibody Database</h1> <h2>Find antibodies validated for Single-Cell Westerns on Milo</h2> </div> <p>Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.</p> <p> If you don't see your antibody listed here, we can help! Please email us your target of interest at <a href="mailto:support@proteinsimple.com">support@proteinsimple.com</a>. </p> <div id="simple_antibody_db" class="p-4 bg-white rounded border"> <h3>Single-Cell Western Validated Antibodies</h3> <hr class="default-hr"> <div id="simple_antibody_count"> Filter Antibodies: (<span id="simple_antibody_count_span">400</span>) </div> <div class="row"> <div class="col-md-6"> <div class="input-group"> <input type="text" name="simple_search" id="simple_search_bar" class="w-75 form-control rounded pr-4" data-url="https://www.bio-techne.com/resources/single-cell-western-antibody-database" placeholder="Search Protein, catalog #, vendor, sample... " value=""/> <span id="simple_search_title_clear"> <span class="icon-x-altx-alt"></span> </span> </div> </div> </div> <div id="simple_antibody_data" class="table-responsive mt-2"> <table class="table table-sm table-bordered"> <thead> <tr> <th scope="col"><a href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?sort=protein_target">Validated Target</a> </th> <th scope="col"><a href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?sort=host_species">Host Species</a> </th> <th scope="col" class="text-nowrap"><a href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?sort=vendor">Vendor / Cat #</a> </th> <th scope="col" class="w-25"><a href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?sort=sample">Sample</a> </th> <th scope="col"><a href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?sort=concentration">Concentration</a> </th> </tr> </thead> <tbody id="simple_tbody_data"> <tr> <td>α-actinin</td> <td>Goat</td> <td class="text-nowrap"> R&D Systems<br/> <a href="https://www.bio-techne.com/p/antibodies/human-mouse-rat-alpha-actinin-1-antibody_af8279" target="_blank" class="a___link-external">AF8279</a> </td> <td>KPC cells</td> <td>100 µg/mL</td> </tr> <tr> <td>α-actinin4</td> <td>Mouse</td> <td class="text-nowrap"> Santa Cruz Biotechnology<br/> sc-390205 </td> <td>HeLa cells</td> <td>100 µg/mL</td> </tr> <tr> <td>α-synuclein</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 4179 </td> <td>SHY5Y cells</td> <td>1:10</td> </tr> <tr> <td>β-tubulin</td> <td>Mouse</td> <td class="text-nowrap"> Genetex<br/> GTX11312 </td> <td>Primary Her2+ breast tumor cells</td> <td>1:10</td> </tr> <tr> <td>β-tubulin*</td> <td>Rabbit</td> <td class="text-nowrap"> Abcam<br/> ab6046 </td> <td>Murine pancreatic cancer cells</td> <td>1:40</td> </tr> <tr> <td>β-tubulin*</td> <td>Mouse</td> <td class="text-nowrap"> Genscript<br/> A-01717-40 </td> <td>Human pluripotent stem cells</td> <td>1:40</td> </tr> <tr> <td>βIII-tubulin (neuronal)</td> <td>Mouse</td> <td class="text-nowrap"> Sigma Aldrich<br/> T8578 </td> <td>Human iPSCs</td> <td>100 µg/mL</td> </tr> <tr> <td>Actin</td> <td>Mouse</td> <td class="text-nowrap"> Developmental Studies Hybridoma Bank<br/> 224-236-1 </td> <td>Dictyostelium discoideum</td> <td>100 µg/mL</td> </tr> <tr> <td>Akt (pan)</td> <td>Mouse</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 2920 </td> <td>C2C12 cells</td> <td>1:10</td> </tr> <tr> <td>Akt (pan)</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 4685 </td> <td>C2C12 cells</td> <td>1:10</td> </tr> <tr> <td>Akt (pan) (C67E7)</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 4691 </td> <td>CRC tumor samples and human cell lines</td> <td>1:10</td> </tr> <tr> <td>Akt (pan)*</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 4691 </td> <td>C2C12 cells</td> <td>1:10</td> </tr> <tr> <td>Albumin</td> <td>Mouse</td> <td class="text-nowrap"> Abcam<br/> 10241 </td> <td>Hepatocyte co-culture</td> <td>100 µg/mL</td> </tr> <tr> <td>Albumin</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 4929 </td> <td>Hepatocyte co-culture</td> <td>1:10</td> </tr> <tr> <td>ALDH1A1</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 12035 </td> <td>(unpublished)</td> <td>1:10</td> </tr> <tr> <td>alpha-actinin</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 6487 </td> <td>BT474</td> <td></td> </tr> <tr> <td>AML1</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 4336 </td> <td>Jurkat cells</td> <td>1:10</td> </tr> <tr> <td>Anti-mouse Alexa Fluor 555</td> <td>Goat</td> <td class="text-nowrap"> ThermoFisher<br/> A-21422 </td> <td>CRC tumor samples and human cell lines</td> <td>1:20</td> </tr> <tr> <td>Anti-mouse Alexa Fluor 647</td> <td>Goat</td> <td class="text-nowrap"> ThermoFisher<br/> A-21235 </td> <td>CRC tumor samples and human cell lines</td> <td>1:20</td> </tr> <tr> <td>Anti-rabbit Alexa Fluor 555</td> <td>Goat</td> <td class="text-nowrap"> ThermoFisher<br/> A-21428 </td> <td>CRC tumor samples and human cell lines</td> <td>1:20</td> </tr> <tr> <td>Anti-rabbit Alexa Fluor 647</td> <td>Chicken</td> <td class="text-nowrap"> ThermoFisher<br/> A-21443 </td> <td>CRC tumor samples and human cell lines</td> <td>1:20</td> </tr> <tr> <td>APRIL</td> <td>Goat</td> <td class="text-nowrap"> R&D Systems<br/> <a href="https://www.bio-techne.com/p/antibodies/human-april-tnfsf13-antibody_af884" target="_blank" class="a___link-external">AF884</a> </td> <td>MOLT-4 cells</td> <td>1:5</td> </tr> <tr> <td>ASC</td> <td>Rabbit</td> <td class="text-nowrap"> Adipogen<br/> AG-25B-0006-C100 </td> <td>GM-CSF BMDCs</td> <td>50 µg/mL</td> </tr> <tr> <td>ATG5</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 129945 </td> <td>Macrophages</td> <td>1:10</td> </tr> <tr> <td>Atg7</td> <td>Mouse</td> <td class="text-nowrap"> R&D Systems<br/> <a href="https://www.bio-techne.com/p/antibodies/human-mouse-atg7-antibody-683906_mab6608" target="_blank" class="a___link-external">MAB6608</a> </td> <td>HeLa cells</td> <td>100 µg/mL</td> </tr> <tr> <td>Atg7*</td> <td>Rabbit</td> <td class="text-nowrap"> Abcam<br/> ab133528 </td> <td>HeLa cells</td> <td>100 µg/mL</td> </tr> <tr> <td>ATM</td> <td>Mouse</td> <td class="text-nowrap"> Novus Biologicals<br/> <a href="https://www.bio-techne.com/p/antibodies/atm-antibody-2c1_nb100-309" target="_blank" class="a___link-external">NB100-309</a> </td> <td>(unpublished)</td> <td>100 µg/mL</td> </tr> <tr> <td>ATP1A3</td> <td>Mouse</td> <td class="text-nowrap"> Abcam<br/> ab2826 </td> <td>Live primary human cortical neurons</td> <td>100 µg/mL</td> </tr> <tr> <td>ATP1A3/Sodium Potassium ATPase Alpha 3</td> <td>Mouse</td> <td class="text-nowrap"> Novus Biologicals<br/> <a href="https://www.bio-techne.com/p/antibodies/sodium-potassium-atpase-alpha-3-antibody-xvif9-g10_nb300-540" target="_blank" class="a___link-external">NB300-540</a> </td> <td>Live primary human cortical neurons</td> <td>100 µg/mL</td> </tr> <tr> <td>Axl</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 8661 </td> <td>HeLa cells</td> <td>1:10</td> </tr> <tr> <td>BAFF</td> <td>Goat</td> <td class="text-nowrap"> R&D Systems<br/> <a href="https://www.bio-techne.com/p/antibodies/human-baff-blys-tnfsf13b-antibody_af124" target="_blank" class="a___link-external">AF124</a> </td> <td>MOLT-4 cells</td> <td>1:10</td> </tr> <tr> <td>Bcl-xL</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 2764 </td> <td>HeLa cells</td> <td>1:10</td> </tr> <tr> <td>BCL6</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 5650 </td> <td>HeLa cells</td> <td>1:10</td> </tr> <tr> <td>BCL6</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 14895 </td> <td>HeLa cells</td> <td>1:10</td> </tr> <tr> <td>beta-tubulin</td> <td>Mouse</td> <td class="text-nowrap"> Novus Biologicals<br/> <a href="https://www.bio-techne.com/p/antibodies/beta-tubulin-antibody-1b12_nbp2-50061" target="_blank" class="a___link-external">NBP2-50061</a> </td> <td>Jurkat cells</td> <td>50 µg/mL</td> </tr> <tr> <td>beta-tubulin</td> <td>Mouse</td> <td class="text-nowrap"> Novus Biologicals<br/> <a href="https://www.bio-techne.com/p/antibodies/beta-tubulin-antibody-tu-06_nb120-7792" target="_blank" class="a___link-external">NB120-7792</a> </td> <td>Jurkat cells</td> <td>50 µg/mL</td> </tr> <tr> <td>betaIII-tubulin</td> <td>Mouse</td> <td class="text-nowrap"> R&D Systems<br/> <a href="https://www.bio-techne.com/p/antibodies/neuron-specific-beta-iii-tubulin-antibody-tuj-1_mab1195" target="_blank" class="a___link-external">MAB1195</a> </td> <td>neural differentiated iPSCs</td> <td>100 µg/mL</td> </tr> <tr> <td>Blasticidin</td> <td>Rabbit</td> <td class="text-nowrap"> Abcam<br/> ab38307 </td> <td>Transfected HEK293T cells</td> <td>100 µg/mL</td> </tr> <tr> <td>Blimp-1</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 9115 </td> <td>U266 cells</td> <td>1:10</td> </tr> <tr> <td>BRM</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 11966 </td> <td>293T cells</td> <td>1:10</td> </tr> <tr> <td>BTK</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 8547 </td> <td>Primary sorted human spleen lymphocytes</td> <td>1:10</td> </tr> <tr> <td>BTK</td> <td>Mouse</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 56044 </td> <td>Primary sorted human spleen lymphocytes</td> <td>1:10</td> </tr> <tr> <td>c-Jun</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 9165 </td> <td>KPC cells</td> <td>1:10</td> </tr> <tr> <td>c-Myc</td> <td>Rabbit</td> <td class="text-nowrap"> Cell Signaling Technology<br/> 5605 </td> <td>Human pluripotent stem cells</td> <td>1:10</td> </tr> <tr> <td>Calnexin</td> <td>Rabbit</td> <td class="text-nowrap"> Novus Biologicals<br/> <a href="https://www.bio-techne.com/p/antibodies/calnexin-antibody_nbp1-97476" target="_blank" class="a___link-external">NBP1-97476</a> </td> <td>U373 MG cells</td> <td>1:10</td> </tr> <tr> <td>Calnexin</td> <td>Rabbit</td> <td class="text-nowrap"> Enzo Life Sciences<br/> ADI-SPA-865 </td> <td>U373 MG cells</td> <td>1:10</td> </tr> <tr> <td>Cas9</td> <td>Mouse</td> <td class="text-nowrap"> Abcam<br/> ab191468 </td> <td>Mouse lymphocytes</td> <td>1:10</td> </tr> <tr> <td>Caspase 8</td> <td>Mouse</td> <td class="text-nowrap"> Enzo<br/> ADI-AAM-118-E </td> <td>Treated Jurkat cells</td> <td>1:10</td> </tr> <tr> <td>CCL3/MIP1</td> <td>Goat</td> <td class="text-nowrap"> R&D Systems<br/> <a href="https://www.bio-techne.com/p/antibodies/human-ccl3-mip-1alpha-antibody_af-270-na" target="_blank" class="a___link-external">AF-270-NA</a> </td> <td>Differentiated THP-1 treated with LPS and Transport Inhibitors</td> <td>20 µg/mL</td> </tr> <tr> <td>CCR7</td> <td>Rabbit</td> <td class="text-nowrap"> Novus Biologicals<br/> <a href="https://www.bio-techne.com/p/antibodies/ccr7-antibody_nbp2-58753" target="_blank" class="a___link-external">NBP2-58753</a> </td> <td>Jurkat cells</td> <td>100 µg/mL</td> </tr> </tbody> </table> </div> </div> <hr class="default-hr"> <div id="simple_pagination"> <div class="row"> <div class="col-md-6"> <span id="results_per_page_legend">Results Per Page</span> <span class="results_per_page_selected">50</span> <a class="results_per_page_link" href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?num_results=100">100</a> <a class="results_per_page_link" href="https://www.bio-techne.com/resources/single-cell-western-antibody-database?num_results=200">200</a> </div> <div class="col-md-6"> <ul id="simple_pagination" class="pagination float-md-right"> <li class="page-item mr-1 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