A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

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<ul class="nav navbar-nav navbar-right" style="display: inline-flex; align-items: center; margin-left: 20px;"> <li id="language" class="d-none d-xl-inline-flex"> <a href="javascript:"> <div class="current"> <i class="ivu-icon ivu-icon-md-globe"></i> EN </div> </a> <div class="selection"> <a rel="alternate" hreflang="en" href="https://www.peeref.com/works/15544075" > <span>English</span> </a> <a rel="alternate" hreflang="zh" href="https://www.peeref.com/zh/works/15544075" > <span>中文</span> </a> </div> </li> </ul> </ul> </div> </nav> <main> <div id="top-info-banner" class="container-fluid mb-0"> <div class="container"> <div class="d-flex align-items-center" style="margin-top: 30px;"> <span class="text-white"> <strong class="f18">☆</strong> <span class="f16">4.8</span> </span> <span class="mx-3"></span> <span class="tag">Article</span> </div> <h1 class="title title-for-article"> A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity </h1> <div class="help-links-left"> <p class="pub-info"> SCIENCE (2012) </p> </div> </div> </div> <div id="article-sticky-navbar"> <div class="container"> <div class="d-flex justify-content-between flex-wrap flex-md-nowrap"> <div class="d-flex align-items-center mb-2"> <ul class="nav nav-underline f16 font-weight-bold"> <li class="active"> <a href="javascript:;"> Overview </a> </li> <li class=""> <a href="https://www.peeref.com/works/15544075/comments"> Write a Review </a> </li> </ul> </div> <div class="d-flex align-items-center justify-content-md-end flex-wrap flex-md-nowrap"> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://doi.org/10.1126/science.1225829" target="_blank" class="btn btn-warning btn-circle"> <i class="ivu-icon ivu-icon-md-copy f16"></i> <strong>Get Full Text</strong> </a> </div> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://www.peeref.com/works/15544075/add-to-collection" class="btn btn-success btn-circle"> <strong>Add to Collection</strong> </a> 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class="container"> <div class="col-md-4 px-0 pr-md-3"> <div class="f15 panel-box rounded shadow-none border"> <div class="mb-3 pb-3"> <h4 class="mt-0">Journal</h4> <div class="f16"> <h5 class="title f16"> <a href="https://www.peeref.com/journals/7393/science"> SCIENCE </a> </h5> <span> Volume 337, Issue 6096, Pages 816-821 </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Publisher</h4> <div class="f16"> <h5 class="title f16 text-primary"> AMER ASSOC ADVANCEMENT SCIENCE </h5> <div class="my-2"> DOI: 10.1126/science.1225829 </div> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Keywords</h4> <div class="f16"> - </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Categories</h4> <div class="f16"> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Multidisciplinary+Sciences" target="_blank" class="text-dark btn btn-link p-0 text-left"> Multidisciplinary Sciences </a> </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Funding</h4> <div class="f16"> <ol class=""> <li>HHMI</li> <li>Austrian Science Fund [W1207-B09]</li> <li>Univ. of Vienna</li> <li>Swedish Research Council [K2010-57X-21436-01-3, 621-2011-5752-LiMS]</li> <li>Kempe Foundation</li> <li>Umea University</li> <li>Austrian Science Fund (FWF) [W1207] Funding Source: Austrian Science Fund (FWF)</li> <li>Direct For Biological Sciences [950971] Funding Source: National Science Foundation</li> <li>Div Of Molecular and Cellular Bioscience [950971] Funding Source: National Science Foundation</li> </ol> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 text-center">Ask authors/readers for more resources</h4> <div class="requests"> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/file.png" alt=""> </div> <h4>Protocol</h4> <p> <a href="https://www.peeref.com/works/15544075/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/experiment.png" alt=""> </div> <h4>Reagent</h4> <p> <a href="https://www.peeref.com/works/15544075/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> </div> </div> </div> <div class="col-md-8 px-0 pl-md-3"> <div id="article-summary-panel" class="mb-4"> <ul class="nav nav-tabs" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#raw_abstract" data-toggle="tab" class="abstract-tab mx-0 f16 text-dark"> <strong>Abstract</strong> </a> </li> </ul> <div class="tab-content border border-top-0"> <div id="raw_abstract" class="tab-pane active"> <div class="abstract-panel panel-box mb-0 rounded shadow-none"> <div class="f16">Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. At sites complementary to the crRNA-guide sequence, the Cas9 HNH nuclease domain cleaves the complementary strand, whereas the Cas9 RuvC-like domain cleaves the noncomplementary strand. The dual-tracrRNA:crRNA, when engineered as a single RNA chimera, also directs sequence-specific Cas9 dsDNA cleavage. Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.</div> </div> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Authors</h4> <div class="mb-3"> <article-authors tid="15544075" list="[{"name":"Martin Jinek","sequence":1},{"name":"Krzysztof Chylinski","sequence":2},{"name":"Ines Fonfara","sequence":3},{"name":"Michael Hauer","sequence":4},{"name":"Jennifer A. Doudna","sequence":5},{"name":"Emmanuelle Charpentier","sequence":6}]" verified="[]" page="work" ></article-authors> </div> <div class="alert alert-warning mb-0"> <h5 class="mt-0 bg-warning text-dark px-3 rounded d-inline-block"> I am an author on this paper </h5> <div class="font-weight-bold f13"> Click your name to claim this paper and add it to your profile. </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Reviews</h4> <div class="d-flex flex-wrap flex-md-nowrap"> <div class="flex-grow-1"> <h4 class="f16"> Primary Rating <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="The primary rating indicates the level of overall quality for the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap align-items-center alert mb-0"> <div class="d-flex align-items-center justify-content-center"> <Rate disabled allow-half value="4.8" style="font-size: 28px;"></Rate> <strong class="f20 m-3" style="color: #f5a623;">4.8</strong> </div> <div class="text-muted mx-4"> Not enough ratings </div> </div> <h4 class="f16"> Secondary Ratings <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="Secondary ratings independently reflect strengths or weaknesses of the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap alert"> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Novelty</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Significance</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Scientific rigor</h5> <strong class="mx-4">-</strong> </div> </div> </div> <div class="flex-shrink-0"> <div class="border bg-light py-2 px-4"> <h5 class="mb-1">Rate this paper</h5> <Rate class="f24" @on-change="function(value){ location.href='https://www.peeref.com/works/15544075/comments?rating='+value }"></Rate> </div> </div> </div> </div> <div id="collection" class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Recommended</h4> <div class="my-3"> <ul class="nav nav-pills border-bottom pb-3" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#articles_from_related" data-toggle="tab" class="mx-0 f15"> <strong>Related</strong> </a> </li> <li class=""> <a href="#articles_from_authors" data-toggle="tab" class="mx-0 f15"> <strong>From Same Authors</strong> </a> </li> <li class=""> <a href="#articles_from_journal" data-toggle="tab" class="mx-0 f15"> <strong>From Same Journal</strong> </a> </li> </ul> <div class="tab-content"> <div id="articles_from_related" class="tab-pane active"> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Cell Biology </span> </div> <h4> <a href="https://www.peeref.com/works/81883157" class="text-dark hover-underline">IS607 TnpB is a hypercompact RNA-guided DNA endonuclease</a> </h4> <p class="text-ellipsis-2">Hui Yang, Dinshaw J. Patel</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1577.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The study identified a new family of compact RNA-guided DNA endonucleases for genome editing, which is different from other systems, highlighting the diversity of non-Cas genome editing tools. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">CELL RESEARCH</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81883157/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/27832347" class="text-dark hover-underline">Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly</a> </h4> <p class="text-ellipsis-2">Xingchen Xiong, Zhiwen Lu, Lixin Ma, Chao Zhai</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/10620.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Programmable endonucleases, such as Cas and pAgo, can recognize and cleave DNA sequences at nearly any arbitrary site by base pairing with guide RNA or DNA. This review focuses on the use of these enzymes in in vivo and in vitro recombination-based gene cloning methods, and the advantages and disadvantages of using programmable endonucleases for gene cloning are discussed. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOMOLECULES</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/27832347/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/26417566" class="text-dark hover-underline">A bacterial Argonaute from Tepiditoga spiralis with the ability of RNA guided plasmid cleavage</a> </h4> <p class="text-ellipsis-2">Xiaochen Xie, Longyu Wang, Lixin Ma</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1078.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a new Argonaute protein called TsAgo was identified from Tepiditoga spiralis. TsAgo, similar to other reported proteins, prefers 5'OH-gRNA. It has been shown that TsAgo has DNA and RNA cleavage activity, can tolerate mismatches in certain regions of guides, and exhibits a stronger binding affinity for 5'OH-gRNA. Importantly, TsAgo can function as an RNA-guided programmable nuclease to cut plasmid DNA at high temperatures. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26417566/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/33999889" class="text-dark hover-underline">Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis</a> </h4> <p class="text-ellipsis-2">Huifeng Xu, Rui Pan, Weihua Huang, Xi Zhu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/520.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A dual-mode sensing platform based on CRISPR-Cas12a technology has been developed for the detection of OTA in Chinese herbs. The platform shows high sensitivity and good anti-interference ability against complex substrates. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33999889/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biotechnology & Applied Microbiology </span> </div> <h4> <a href="https://www.peeref.com/works/25266251" class="text-dark hover-underline">Characterization and application of a thermophilic Argonaute from archaeon Thermococcus thioreducens</a> </h4> <p class="text-ellipsis-2">Mengjun Fang, Zhipeng Xu, Di Huang, Muhammad Naeem, Xiangcheng Zhu, Zhinan Xu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1214.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study reported the in vitro characterization of an Argonaute protein from Thermococcus thioreducens, demonstrating its application in hepatitis B virus DNA detection. The protein functions as a programmable DNA endonuclease with high efficiency and accuracy at certain temperatures, showing potential in DNA manipulation tools. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOTECHNOLOGY AND BIOENGINEERING</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/25266251/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/33999546" class="text-dark hover-underline">Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis</a> </h4> <p class="text-ellipsis-2">Huifeng Xu, Rui Pan, Weihua Huang, Xi Zhu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/520.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a biosensor for the detection of ochratoxin A (OTA) in traditional Chinese herbs was developed using the CRISPR-Cas12a system and electrochemical probes. The biosensor not only showed high sensitivity, but also demonstrated good anti-interference ability against complex substrates, providing promising prospects for application. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33999546/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biophysics </span> </div> <h4> <a href="https://www.peeref.com/works/85072750" class="text-dark hover-underline">Highly efficient loop cleavage for human papillomavirus detection with a novel thermophilic Argonaute from Thermus brockianus</a> </h4> <p class="text-ellipsis-2">Mengjun Fang, Zhipeng Xu, Fei Yu, Zehua Bao, Minjie Shen, Peijie Shen, Di Huang, Qiang Shu, Zhinan Xu, Xiangming Fang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1203.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study characterized a novel thermophilic Argonaute protein, TbAgo, which can cleave DNA efficiently at 65°C and is compatible with the LAMP method. Based on this, an AMEND isothermal nucleic acid detection platform was developed, which can simultaneously detect HPV16 and 18 DNA with a detection limit of 1 aM within 30 minutes. In addition, a two-step microfluidic chip was designed to further improve the detection efficiency. This study provides a new idea and method for efficient DNA detection. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOSENSORS & BIOELECTRONICS</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/85072750/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/28745020" class="text-dark hover-underline">A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo</a> </h4> <p class="text-ellipsis-2">Burcu Bestas, Sandra Wimberger, Dmitrii Degtev, Alexandra Madsen, Antje K. Rottner, Fredrik Karlsson, Sergey Naumenko, Megan Callahan, Julia Liz Touza, Margherita Francescatto, Carl Ivar Moller, Lukas Badertscher, Songyuan Li, Silvia Cerboni, Niklas Selfjord, Elke Ericson, Euan Gordon, Mike Firth, Krzysztof Chylinski, Amir Taheri-Ghahfarokhi, Mohammad Bohlooly-Y, Mike Snowden, Menelaos Pangalos, Barrett Nuttall, Pinar Akcakaya, Grzegorz Sienski, Marcello Maresca</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/8411.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The authors developed a Duplex Sequencing-based workflow to increase the sensitivity for detecting off-target mutations in Cas9 editing. They also identified a high-fidelity Cas9 variant, PsCas9, with improved specificity compared to the widely used SpCas9. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE COMMUNICATIONS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28745020/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/23768923" class="text-dark hover-underline">Different modes of spacer acquisition by the Staphylococcus epidermidis type III-A CRISPR-Cas system</a> </h4> <p class="text-ellipsis-2">Naama Aviram, Ashley N. Thornal, David Zeevi, Luciano A. Marraffini</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/6280.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR-Cas systems provide prokaryotic organisms with an adaptive defense mechanism that acquires immunological memories of infections. This study investigated the spacer acquisition process in the type III-A CRISPR-Cas system of Staphylococcus epidermidis. It was found that this type of system uses Cas1 and Cas2 to integrate spacers from chromosomal terminus and free dsDNA ends, similar to type I and II systems. Additionally, a different mode of spacer acquisition from rRNA and tRNA loci was identified. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NUCLEIC ACIDS RESEARCH</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/23768923/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83074472" class="text-dark hover-underline">Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells</a> </h4> <p class="text-ellipsis-2">Anna Nemudraia, Artem Nemudryi, Blake Wiedenheft</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7393.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study achieved precise RNA manipulation in human cells by using RNA breaks generated with CRISPR complexes, which can be used to restore gene function and provides a potential technology for therapeutic applications. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83074472/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Cell Biology </span> </div> <h4> <a href="https://www.peeref.com/works/26118761" class="text-dark hover-underline">Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease</a> </h4> <p class="text-ellipsis-2">Yujue Wang, Yannan Wang, Deng Pan, Haopeng Yu, Yifei Zhang, Weizhong Chen, Fan Li, Zhaowei Wu, Quanjiang Ji</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/8772.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The miniature CRISPR-Cas12f system from Syntrophomonas palmitatica, known as SpaCas12f1, showed natural activity and efficient genome editing capabilities in bacteria. Through systematic engineering, it was transformed into a highly efficient genome editor, providing new possibilities for research and clinical applications. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">CELL REPORTS</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26118761/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Biotechnology & Applied Microbiology </span> </div> <h4> <a href="https://www.peeref.com/works/83280945" class="text-dark hover-underline">RNA-guided genome engineering: paradigm shift towards transposons</a> </h4> <p class="text-ellipsis-2">Chin-Wei Chang, Vy Anh Truong, Nam Ngoc Pham, Yu-Chen Hu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7915.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR-Cas systems have revolutionized genome engineering, but they have limitations. Tn7-like transposons and OMEGA proteins are exciting genome editing tools with the potential to improve editing precision and efficiency. This review discusses their recent developments and applications in prokaryotic and eukaryotic cells, and compares their pros and cons with other CRISPR systems. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">TRENDS IN BIOTECHNOLOGY</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83280945/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/82596097" class="text-dark hover-underline">Type III CRISPR-Cas: beyond the Cas10 effector complex</a> </h4> <p class="text-ellipsis-2">Gianna Stella, Luciano Marraff</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7914.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article mainly introduces the composition and mechanism of action of the type III CRISPR-Cas immune system, as well as the functions of some accessory proteins in this system, such as accessory nucleases, ring nucleases, and CRISPR-associated proteases. These studies help to deeply understand the immune system and defense mechanisms of prokaryotes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">TRENDS IN BIOCHEMICAL SCIENCES</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82596097/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/83751019" class="text-dark hover-underline">Engineering IscB to develop highly efficient miniature editing tools in mammalian cells and embryos</a> </h4> <p class="text-ellipsis-2">Niannian Xue, Dishan Hong, Dan Zhang, Qian Wang, Shun Zhang, Lei Yang, Xi Chen, Yongmei Li, Honghui Han, Chunyi Hu, Mingyao Liu, Gaojie Song, Yuting Guan, Liren Wang, Yifan Zhu, Dali Li</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/5951.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> IscB proteins have low activity in mammalian cells, but engineered eIscB-D/euRNA system shows significantly increased activity by introducing residual substitutions and fusing DNA-binding protein domains, which can be used for efficient base editing and has high activity in mouse cell lines and embryos, enabling the generation of disease models. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">MOLECULAR CELL</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83751019/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/26025519" class="text-dark hover-underline">Molecular basis of cyclic tetra-oligoadenylate processing by small standalone CRISPR-Cas ring nucleases</a> </h4> <p class="text-ellipsis-2">Rafael Molina, Ricardo Garcia-Martin, Blanca Lopez-Mendez, Anne Louise Gron Jensen, J. Rafael Ciges-Tomas, Javier Marchena-Hurtado, Stefano Stella, Guillermo Montoya</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/6280.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Standalone ring nucleases are CRISPR ancillary proteins that downregulate the immune response of Type III CRISPR-Cas systems. A study on Sulfolobus islandicus genome identified two genes coding for different length polypeptides with this function. The analysis of one of these polypeptides revealed its structure and the key role it plays in the cleavage process. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NUCLEIC ACIDS RESEARCH</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26025519/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_authors" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/84845615" class="text-dark hover-underline">Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes</a> </h4> <p class="text-ellipsis-2">Kenny Jungfer, Stefan Moravcik, Carmela Garcia-Doval, Anna Knoerlein, Jonathan Hall, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study used cryoelectron microscopy to observe the mechanism of cA6 biosynthesis by the Csm effector complex from Enterococcus italicus, revealing the molecular mechanism that determines the identity of the cOA product. It provides a comprehensive understanding of the dynamics of cOA synthesis in type III CRISPR-Cas systems and provides potential avenues for its engineering. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NUCLEIC ACIDS RESEARCH</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84845615/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/85067303" class="text-dark hover-underline">DNA end sensing and cleavage by the Shedu anti-phage defense system</a> </h4> <p class="text-ellipsis-2">Luuk Loeff, Alexander Walter, Gian Tizio Rosalen, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study reveals the complex host defense mechanisms deployed by prokaryotes in innate anti-phage immunity. Shedu is a single-component defense system composed of a putative nuclease SduA. The researchers report the cryoelectron microscopy (cryo-EM) structures of apo- and double-stranded DNA (dsDNA)-bound tetrameric SduA assemblies, revealing that the N-terminal domains of SduA form a clamp that recognizes free DNA ends. End binding positions the DNA over the PD-(D/E)XK nuclease domain, resulting in dsDNA nicking at a fixed distance from the 50 end. The end-directed DNA nicking activity of Shedu prevents the propagation of linear DNA in vivo. Finally, the study shows that phages escape Shedu immunity by suppressing their recombination-dependent DNA replication pathway. In conclusion, these results define the antiviral mechanism of Shedu systems, emphasizing that the recognition of pathogen-specific nucleic acid structures is a conserved feature of innate immunity across all domains of life. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">CELL</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/85067303/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/82028295" class="text-dark hover-underline">Past, present, and future of CRISPR genome editing technologies</a> </h4> <p class="text-ellipsis-2">Martin Pacesa, Oana Pelea, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This review discusses the current state of CRISPR gene editing technologies in research and therapy, including their limitations and technological innovations developed to address them. It also examines and summarizes the current applications of gene editing in human health and therapeutics, and finally outlines the potential future developments of these technologies. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">CELL</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82028295/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/82290316" class="text-dark hover-underline">An alpha-helical lid guides the target DNA toward catalysis in CRISPR-Cas12a</a> </h4> <p class="text-ellipsis-2">Aakash Saha, Mohd Ahsan, Pablo R. Arantes, Michael Schmitz, Christelle Chanez, Martin Jinek, Giulia Palermo</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study reveals the crucial role of an alpha-helical lid in the CRISPR-Cas12a system in guiding the DNA target strand towards the RuvC catalytic core, a process essential for achieving double-stranded DNA cleavage. The findings contribute to a deeper understanding of the mechanism of action of Cas12a and provide a theoretical basis for the further development of genome editing technologies. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE COMMUNICATIONS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82290316/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83101947" class="text-dark hover-underline">RNA-guided RNA silencing by an Asgard archaeal Argonaute</a> </h4> <p class="text-ellipsis-2">Carolien Bastiaanssen, Pilar Bobadilla Ugarte, Kijun Kim, Giada Finocchio, Yanlei Feng, Todd A. Anzelon, Stephan Kostlbacher, Daniel Tamarit, Thijs J. G. Ettema, Martin Jinek, Ian J. Macrae, Chirlmin Joo, Daan C. Swarts, Fabai Wu</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Argonaute proteins play crucial roles in eukaryotes and are divided into AGO and PIWI clades. A deep-branching PIWI protein, HrAgo1, from Asgard archaea shares a common origin with eukaryotic PIWI proteins and can mediate RNA cleavage and gene silencing. The structure and features of HrAgo1 suggest that it may represent an ancestral form of eukaryotic AGO and PIWI proteins. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE COMMUNICATIONS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83101947/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83595941" class="text-dark hover-underline">Massively parallel analysis of single-molecule dynamics on next-generation sequencing chips</a> </h4> <p class="text-ellipsis-2">J. Aguirre Rivera, G. Mao, A. Sabantsev, M. Panfilov, Q. Hou, M. Lindell, C. Chanez, F. Ritort, M. Jinek, S. Deindl</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Single-molecule techniques are good at characterizing complex dynamics, but usually limited to a small number of samples. MUSCLE combines single-molecule fluorescence microscopy and next-generation sequencing to enable multiplexed observations of complex dynamics. By using MUSCLE, researchers analyzed the sequence dependence of DNA hairpin properties and Cas9-induced target DNA unwinding-rewinding dynamics, and found many target sequences with unexpected behaviors. This method is expected to be used to explore the mechanisms of many fundamental biological processes. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83595941/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/84316769" class="text-dark hover-underline">Molecular mechanism of plasmid elimination by the DdmDE defense system</a> </h4> <p class="text-ellipsis-2">Luuk Loeff, David W. Adams, Christelle Chanez, Sandrine Stutzmann, Laurie Righi, Melanie Blokesch, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study used cryogenic electron microscopy to determine the mechanism basis of DdmDE plasmid defense. It found that DdmE binding to DNA triggers the disassociation of DdmD dimer and its loading onto the nontarget DNA strand, and DdmD translocates in the 5' to 3' direction while partially degrading plasmid DNA. These findings provide critical insights into the mechanism of DdmDE systems in plasmid elimination. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84316769/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/33828360" class="text-dark hover-underline">Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2</a> </h4> <p class="text-ellipsis-2">Kenny Jungfer, Annina Sigg, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study reveals the structure and mechanism of the Thermoanaerobacter brockii Can2 enzyme in type III CRISPR-Cas immunity. The enzyme exhibits selective activity against single-stranded DNA and RNA, and achieves substrate specificity for CA dinucleotides through specific binding sites and molecular interactions. Furthermore, a molecular interaction relay between the cA4 binding site and the nuclease catalytic site is identified, enabling catalytic activation. These findings provide important insights into the function and applications of Can2 nucleases. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NUCLEIC ACIDS RESEARCH</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33828360/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Correction </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/28603299" class="text-dark hover-underline">R-loop formation and conformational activation mechanisms of Cas9 (vol 609, pg 191, 2022)</a> </h4> <p class="text-ellipsis-2">Martin Pacesa, Luuk Loeff, Irma Querques, Lena M. Muckenfuss, Marta Sawicka, Martin Jinek</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28603299/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/28603803" class="text-dark hover-underline">Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility</a> </h4> <p class="text-ellipsis-2">Lukas Schmidheini, Nicolas Mathis, Kim Fabiano Marquart, Tanja Rothgangl, Lucas Kissling, Desiree Bock, Christelle Chanez, Jingrui Priscilla Wang, Martin Jinek, Gerald Schwank</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR-Cas9 genome engineering is a powerful technology for correcting genetic diseases. In this study, researchers used phage-assisted continuous directed evolution to expand the PAM compatibility of CjCas9, resulting in a new variant called evoCjCas9. This variant can recognize a broader range of PAM sequences and has higher nuclease activity. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE CHEMICAL BIOLOGY</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28603803/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Meeting Abstract </span> <span class="d-inline-block badge badge-cyan"> Biotechnology & Applied Microbiology </span> </div> <h4> <a href="https://www.peeref.com/works/35016854" class="text-dark hover-underline">Conformational Control of Cas Endonucleases by CRISPR Hybrid RNA-DNA Guides Mitigates Off-Target Activity in T Cells</a> </h4> <p class="text-ellipsis-2">Paul Donohoue, Martin Pacesa, Elaine Lau, Bastien Vidal, Matthew Irby, David Nyer, Tomer Rotstein, Lynda Banh, Mckenzi Toh, Jason Gibson, Bryan Kohrs, Kevin Baek, Arthur Owen, Euan Slorach, Megan van Overbeek, Chris Fuller, Andy May, Martin Jinek, Peter Cameron</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">MOLECULAR THERAPY</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/35016854/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Meeting Abstract </span> <span class="d-inline-block badge badge-cyan"> Biophysics </span> </div> <h4> <a href="https://www.peeref.com/works/35171507" class="text-dark hover-underline">Multiplexed single-molecule experiments reveal Cas9 nucleosome invasion dynamics</a> </h4> <p class="text-ellipsis-2">Kristina Makasheva, Louise Bryan, Martin Jinek, Beat Fierz</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOPHYSICAL JOURNAL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/35171507/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Meeting Abstract </span> <span class="d-inline-block badge badge-cyan"> Biophysics </span> </div> <h4> <a href="https://www.peeref.com/works/35174929" class="text-dark hover-underline">Multi-microsecond molecular dynamics unveils the mechanism of DNA traversal within CRISPR-Cas12a</a> </h4> <p class="text-ellipsis-2">Aakash Saha, Pablo R. Arantes, Mohd Ahsan, Martin Jinek, Giulia Palermo</p> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOPHYSICAL JOURNAL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/35174929/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/25808760" class="text-dark hover-underline">Structural basis for Cas9 off-target activity</a> </h4> <p class="text-ellipsis-2">Martin Pacesa, Chun-Han Lin, Antoine Clery, Aakash Saha, Pablo R. Arantes, Katja Bargsten, Matthew J. Irby, Frederic H. -T. Allain, Giulia Palermo, Peter Cameron, Paul D. Donohoue, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The crystallographic structures of Cas9 bound to off-target substrates reveal that noncanonical base-pairing interactions within the guide:off-target heteroduplex enable off-target binding. Single-nucleotide deletions in off-target substrates are accommodated by base skipping or multiple noncanonical base pairs. PAM-distal mismatches result in duplex unpairing and induce a conformational change in the Cas9 REC lobe. These insights contribute to the improved rational design of guide RNAs and off-target prediction algorithms. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">CELL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/25808760/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/26015505" class="text-dark hover-underline">R-loop formation and conformational activation mechanisms of Cas9</a> </h4> <p class="text-ellipsis-2">Martin Pacesa, Luuk Loeff, Irma Querques, Lena M. Muckenfuss, Marta Sawicka, Martin Jinek</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Researchers have used cryo-electron microscopy to observe the structural changes that occur during the binding and activation of Cas9. These findings provide a valuable structural framework for understanding the conformational checkpoint mechanism of Cas9 and may guide the development of new Cas9 variants and guide RNA designs with enhanced specificity and activity. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">NATURE</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26015505/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_journal" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/84311622" class="text-dark hover-underline">Climate change exacerbates the environmental impacts of agriculture</a> </h4> <p class="text-ellipsis-2">Yi Yang, David Tilman, Zhenong Jin, Pete Smith, Christopher B. Barrett, Yong-Guan Zhu, Jennifer Burney, Paolo D'Odorico, Peter Fantke, Joe Fargione, Jacques C. Finlay, Maria Cristina Rulli, Lindsey Sloat, Kees Jan van Groenigen, Paul C. West, Lewis Ziska, Anna M. Michalak, David B. Lobell, Michael Clark, Jed Colquhoun, Teevrat Garg, Karen A. Garrett, Camilla Geels, Rebecca R. Hernandez, Mario Herrero, William D. Hutchison, Meha Jain, Jacob M. Jungers, Beibei Liu, Nathaniel D. Mueller, Ariel Ortiz-Bobea, Jacob Schewe, Jie Song, Julie Verheyen, Peter Vitousek, Yoshihide Wada, Longlong Xia, Xin Zhang, Minghao Zhuang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Agriculture's environmental impacts are expanding due to population, economic growth, and dietary changes. Climate change further amplifies these impacts, causing problems such as decreased agricultural productivity and increased soil erosion. Therefore, a transition to sustainable, climate-resilient agricultural systems is needed, which requires investment and innovation. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84311622/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83166782" class="text-dark hover-underline">Strong-bonding hole-transport layers reduce ultraviolet degradation of perovskite solar cells</a> </h4> <p class="text-ellipsis-2">Chengbin Fei, Anastasia Kuvayskaya, Xiaoqiang Shi, Mengru Wang, Zhifang Shi, Haoyang Jiao, Timothy J. Silverman, Michael Owen-Bellini, Yifan Dong, Yeming Xian, Rebecca Scheidt, Xiaoming Wang, Guang Yang, Hangyu Gu, Nengxu Li, Connor J. Dolan, Zhewen J. D. Deng, Deniz N. Cakan, David P. Fenning, Yanfa Yan, Matthew C. Beard, Laura T. Schelhas, Alan Sellinger, Jinsong Huang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> The LEDs used in indoor testing of perovskite solar cells do not expose them to sufficient UV radiation. This study reports the degradation mechanisms of these cells under unfiltered sunlight and LEDs, finding that weak chemical bonding causes accelerated A-site cation migration rather than direct HTM degradation. EtCz3EPA enhances the bonding in the perovskite/HTM/TCO region, and the hybrid HTM improves the UV stability and efficiency of perovskite devices. The perovskite minimodule maintains a high operational efficiency after outdoor testing. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83166782/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83126540" class="text-dark hover-underline">Fast growth of single-crystal covalent organic frameworks for laboratory x-ray diffraction</a> </h4> <p class="text-ellipsis-2">Jing Han, Jie Feng, Jia Kang, Jie-Min Chen, Xin-Yu Du, San-Yuan Ding, Lin Liang, Wei Wang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed a method for the rapid growth of high-quality single-crystalline COFs. By using the CF3COOH/CF3CH2NH2 protocol, single-crystalline COFs with a size of up to 150 microns can be harvested within 1-2 days, and the structures of 16 high-quality single-crystalline COFs were determined by laboratory single-crystalline X-ray diffraction, including uncommon network interpenetration. The structural evolution details of conformational isomers and host-guest interactions can be determined at the atomic level. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83126540/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83126618" class="text-dark hover-underline">Anthropogenic climate change has influenced global river flow seasonality</a> </h4> <p class="text-ellipsis-2">Hong Wang, Junguo Liu, Megan Klaar, Aifang Chen, Lukas Gudmundsson, Joseph Holden</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study uses apportionment entropy to assess the nonuniformity of river flow across seasons and finds that about 21% of long-term river gauging stations worldwide have experienced significant changes in the distribution of seasonal river flow, two-thirds of which are unrelated to trends in annual mean discharge. In the northern high latitudes, the river flow seasonality has weakened significantly, which is directly related to anthropogenic climate forcing. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83126618/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83128951" class="text-dark hover-underline">Shearing brittle intermetallics enhances cryogenic strength and ductility of steels</a> </h4> <p class="text-ellipsis-2">Feng Wang, Miao Song, Mohamed N. Elkot, Ning Yao, Binhan Sun, Min Song, Zhangwei Wang, Dierk Raabe</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study reports the dislocation cutting of B2 nanoprecipitates in a lightweight compositionally complex steel during cryogenic tensile loading. Shearing is enabled by the high strength level for dislocation glide within the austenitic matrix. This mechanism harnesses the strengthening of nanoprecipitates and introduces ductility. The steel shows ultrahigh cryogenic tensile strength and remarkable tensile elongation, providing a new strategy for designing high-performance structural materials. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83128951/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83138060" class="text-dark hover-underline">N-type semiconducting hydrogel</a> </h4> <p class="text-ellipsis-2">Peiyun Li, Wenxi Sun, Jiulong Li, Ju-Peng Chen, Xinyue Wang, Zi Mei, Guanyu Jin, Yuqiu Lei, Ruiyun Xin, Mo Yang, Jingcao Xu, Xiran Pan, Cheng Song, Xin-Yu Deng, Xun Lei, Kai Liu, Xiu Wang, Yuting Zheng, Jia Zhu, Shixian Lv, Zhi Zhang, Xiaochuan Dai, Ting Lei</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This text mainly introduces a kind of hydrogel based on water-soluble n-type semiconducting polymer, which has semiconductor capabilities and can be used to manufacture electronic devices, such as complementary logic circuits and signal amplifiers, and has good bioadhesion and biocompatibility, and can sense and amplify small signals. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83138060/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83105294" class="text-dark hover-underline">A national-scale assessment of land subsidence in China's major cities</a> </h4> <p class="text-ellipsis-2">Zurui Ao, Xiaomei Hu, Shengli Tao, Xie Hu, Guoquan Wang, Mingjia Li, Fang Wang, Litang Hu, Xiuyu Liang, Jingfeng Xiao, Asadilla Yusup, Wenhua Qi, Qinwei Ran, Jiayi Fang, Jinfeng Chang, Zhenzhong Zeng, Yongshuo Fu, Baolin Xue, Ping Wang, Kefei Zhao, Le Li, Wenkai Li, Yumei Li, Mi Jiang, Yuanhe Yang, Haihua Shen, Xia Zhao, Yue Shi, Bo Wu, Zhengbing Yan, Mengjia Wang, Yanjun Su, Tianyu Hu, Qin Ma, Hao Bai, Lijun Wang, Ziyan Yang, Yuhao Feng, Danhua Zhang, Erhan Huang, Jiamin Pan, Huiying Ye, Chen Yang, Yanwei Qin, Chenqi He, Yanpei Guo, Kai Cheng, Yu Ren, Haitao Yang, Chengyang Zheng, Jiangling Zhu, Shaopeng Wang, Chengjun Ji, Biao Zhu, Hongyan Liu, Zhiyao Tang, Zhiheng Wang, Shuqing Zhao, Yanhong Tang, Hanfa Xing, Qinghua Guo, Yu Liu, Jingyun Fang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study indicates that China's urbanization may be threatened by land subsidence. 45% of urban lands are subsiding faster than 3mm/year, and 16% are subsiding faster than 10mm/year, affecting 29% and 7% of the urban population respectively. The subsidence is related to factors such as groundwater withdrawal and the weight of buildings. By 2120, 22% to 26% of the coastal lands will have a relative elevation lower than sea level, hosting 9% to 11% of the coastal population. The study emphasizes the need to strengthen protective measures to mitigate potential damages. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83105294/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83279233" class="text-dark hover-underline">Additive manufacturing of highly entangled polymer networks</a> </h4> <p class="text-ellipsis-2">Abhishek P. Dhand, Matthew D. Davidson, Hannah M. Zlotnick, Thomas J. Kolibaba, Jason P. Killgore, Jason A. Burdick</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A facile strategy is proposed to achieve high monomer conversion and additive manufacturing of highly entangled hydrogels and elastomers by combining light and dark polymerization. This method enables the printing of high-resolution and multimaterial structures with features such as spatially programmed adhesion to wet tissues, without the need for additional stimuli. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83279233/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/84318920" class="text-dark hover-underline">Soft hydrogel semiconductors with augmented biointeractive functions</a> </h4> <p class="text-ellipsis-2">Yahao Dai, Shinya Wai, Pengju Li, Naisong Shan, Zhiqiang Cao, Yang Li, Yunfei Wang, Youdi Liu, Wei Liu, Kan Tang, Yuzi Liu, Muchuan Hua, Songsong Li, Nan Li, Shivani Chatterji, H. Christopher Fry, Sean Lee, Cheng Zhang, Max Weires, Sean Sutyak, Jiuyun Shi, Chenhui Zhu, Jie Xu, Xiaodan Gu, Bozhi Tian, Sihong Wang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> We incorporated water-insoluble polymer semiconductors into double-network hydrogels, which possess tissue-level moduli, stretchability, and high charge carrier mobility. When interfaced with biological tissues, these hydrogels can alleviate immune reactions. The high porosity of the hydrogels enhances molecular interactions, enabling photomodulation and volumetric biosensing. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84318920/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/84334839" class="text-dark hover-underline">Developing fatigue-resistant ferroelectrics using interlayer sliding switching</a> </h4> <p class="text-ellipsis-2">Renji Bian, Ri He, Er Pan, Zefen Li, Guiming Cao, Peng Meng, Jiangang Chen, Qing Liu, Zhicheng Zhong, Wenwu Li, Fucai Liu</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> We report a fatigue-free ferroelectric system based on the sliding ferroelectricity of bilayer 3R-MoS2. This system shows no wake-up effect at low cycles and no significant fatigue effect after 106 switching cycles, and the total stress time under an electric field is up to 105 s. Theoretical calculations reveal that the fatigue-free feature is due to the immobile charge defects in sliding ferroelectricity. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84334839/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/85268069" class="text-dark hover-underline">Insulating electromagnetic-shielding silicone compound enables direct potting electronics</a> </h4> <p class="text-ellipsis-2">Xinfeng Zhou, Peng Min, Yue Liu, Meng Jin, Zhong-Zhen Yu, Hao-Bin Zhang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This text proposes a novel electromagnetic interference shielding material using a microcapacitor structure model composed of conductive fillers and a polymer dielectric layer, which solves the short-circuit risk of traditional materials and has high resistivity, shielding performance, thermal conductivity, and insulating properties, and can be directly used for packaging electronic devices. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/85268069/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83127490" class="text-dark hover-underline">Kink bands promote exceptional fracture resistance in a NbTaTiHf refractory medium-entropy alloy</a> </h4> <p class="text-ellipsis-2">David H. Cook, Punit Kumar, Madelyn I. Payne, Calvin H. Belcher, Pedro Borges, Wenqing Wang, Flynn Walsh, Zehao Li, Arun Devaraj, Mingwei Zhang, Mark Asta, Andrew M. Minor, Enrique J. Lavernia, Diran Apelian, Robert O. Ritchie</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study investigated the strength and fracture toughness of single-phase body-centered cubic refractory medium- or high-entropy alloys, finding that the NbTaTiHf alloy has a high fracture toughness due to the dynamic competition of dislocations and the formation of kink bands, which helps suppress strain hardening and distribute damage. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83127490/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83127492" class="text-dark hover-underline">Size, distribution, and vulnerability of the global soil inorganic carbon</a> </h4> <p class="text-ellipsis-2">Yuanyuan Huang, Xiaodong Song, Ying-Ping Wang, Josep G. Canadell, Yiqi Luo, Philippe Ciais, Anping Chen, Songbai Hong, Yugang Wang, Feng Tao, Wei Li, Yiming Xu, Reza Mirzaeitalarposhti, Heba Elbasiouny, Igor Savin, Dmitry Shchepashchenko, Raphael A. Viscarra Rossel, Daniel S. Goll, Jinfeng Chang, Benjamin Z. Houlton, Huayong Wu, Fei Yang, Xiaoming Feng, Yongzhe Chen, Yu Liu, Shuli Niu, Gan-Lin Zhang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study quantified the global soil inorganic carbon (SIC) stocks and distributions through field measurements and model analyses, and predicted the impacts of soil acidification on SIC under future scenarios. In addition, the study found that a large amount of inorganic carbon is lost to inland waters through soils annually, which has important implications for the carbon dynamics in the atmosphere and hydrosphere. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83127492/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83148106" class="text-dark hover-underline">Aqueous synthesis of perovskite precursors for highly efficient perovskite solar cells</a> </h4> <p class="text-ellipsis-2">Peide Zhu, Deng Wang, Yong Zhang, Zheng Liang, Jingbai Li, Jie Zeng, Jiyao Zhang, Yintai Xu, Siying Wu, Zhixin Liu, Xianyong Zhou, Bihua Hu, Feng He, Lin Zhang, Xu Pan, Xingzhu Wang, Nam-Gyu Park, Baomin Xu</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Aqueous-synthesized perovskite microcrystals were proposed as precursor materials for PSCs, enabling large-scale production of high-purity formamidinium lead iodide microcrystals. Using these purified precursors, a PCE of 25.6% was achieved in inverted PSCs, with high stability after 1000 hours of continuous simulated solar illumination. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83148106/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Multidisciplinary Sciences </span> </div> <h4> <a href="https://www.peeref.com/works/83162132" class="text-dark hover-underline">Ultrahigh energy storage in high-entropy ceramic capacitors with polymorphic relaxor phase</a> </h4> <p class="text-ellipsis-2">Min Zhang, Shun Lan, Bing B. Yang, Hao Pan, Yi Q. Liu, Qing H. Zhang, Jun L. Qi, Di Chen, Hang Su, Di Yi, Yue Y. Yang, Rui Wei, Hong D. Cai, Hao J. Han, Lin Gu, Ce-Wen Nan, Yuan-Hua Lin</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A high-entropy design strategy is proposed to fabricate multilayer ceramic capacitors with high energy density and high efficiency. By reducing the domain-switching barriers and enhancing the breakdown strength, this strategy achieves a synergistic effect, resulting in high energy density and ultrahigh efficiency in MLCCs. This approach may be applicable to the design of high-performance dielectrics. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SCIENCE</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83162132/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="modal fade" id="export-citation" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"><span>×</span></button> <h4 class="modal-title">Export Citation <b class="text-primary"></b></h4> </div> <div class="modal-body"> <div class="my-3 px-4 f16"> <form action="https://www.peeref.com/works/citation/download" method="GET" target="_blank"> <div class="radio"> <label> <input type="radio" name="fileType" 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A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity - Peeref