Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis

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<ul class="nav navbar-nav navbar-right" style="display: inline-flex; align-items: center; margin-left: 20px;"> <li id="language" class="d-none d-xl-inline-flex"> <a href="javascript:"> <div class="current"> <i class="ivu-icon ivu-icon-md-globe"></i> EN </div> </a> <div class="selection"> <a rel="alternate" hreflang="en" href="https://www.peeref.com/works/33999889" > <span>English</span> </a> <a rel="alternate" hreflang="zh" href="https://www.peeref.com/zh/works/33999889" > <span>中文</span> </a> </div> </li> </ul> </ul> </div> </nav> <main> <div id="top-info-banner" class="container-fluid mb-0"> <div class="container"> <div class="d-flex align-items-center" style="margin-top: 30px;"> <span class="text-white"> <strong class="f18">☆</strong> <span class="f16">4.6</span> </span> <span class="mx-3"></span> <span class="tag">Article</span> </div> <h1 class="title title-for-article"> Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis </h1> <div class="help-links-left"> <p class="pub-info"> ANALYTICAL METHODS (2023) </p> </div> </div> </div> <div id="article-sticky-navbar"> <div class="container"> <div class="d-flex justify-content-between flex-wrap flex-md-nowrap"> <div class="d-flex align-items-center mb-2"> <ul class="nav nav-underline f16 font-weight-bold"> <li class="active"> <a href="javascript:;"> Overview </a> </li> <li class=""> <a href="https://www.peeref.com/works/33999889/comments"> Write a Review </a> </li> </ul> </div> <div class="d-flex align-items-center justify-content-md-end flex-wrap flex-md-nowrap"> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://doi.org/10.1039/d3ay01025b" target="_blank" class="btn btn-warning btn-circle"> <i class="ivu-icon ivu-icon-md-copy f16"></i> <strong>Get Full Text</strong> </a> </div> <div class="mr-3 mt-3 mt-md-0 flex-shrink-0"> <a href="https://www.peeref.com/works/33999889/add-to-collection" class="btn btn-success btn-circle"> 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</div> </div> <div id="article-details" class="container"> <div class="col-md-4 px-0 pr-md-3"> <div class="f15 panel-box rounded shadow-none border"> <div class="mb-3 pb-3"> <h4 class="mt-0">Journal</h4> <div class="f16"> <h5 class="title f16"> <a href="https://www.peeref.com/journals/520/analytical-methods"> ANALYTICAL METHODS </a> </h5> <span> Volume 15, Issue 35, Pages 4518-4523 </span> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Publisher</h4> <div class="f16"> <h5 class="title f16 text-primary"> ROYAL SOC CHEMISTRY </h5> <div class="my-2"> DOI: 10.1039/d3ay01025b </div> </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Keywords</h4> <div class="f16"> - </div> </div> <div class="mb-3 pb-3"> <h4 class="mt-0">Categories</h4> <div class="f16"> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Chemistry%2C+Analytical" target="_blank" class="text-dark btn btn-link p-0 text-left"> Chemistry, Analytical </a> </span> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Food+Science+%26+Technology" target="_blank" class="text-dark btn btn-link p-0 text-left"> Food Science & Technology </a> </span> <span class="d-block"> <a href="https://www.peeref.com/works/list?category=Spectroscopy" target="_blank" class="text-dark btn btn-link p-0 text-left"> Spectroscopy </a> </span> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 text-center">Ask authors/readers for more resources</h4> <div class="requests"> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/file.png" alt=""> </div> <h4>Protocol</h4> <p> <a href="https://www.peeref.com/works/33999889/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> <div class="requests-item"> <div class="icon"> <img src="https://peeref-open.s3.amazonaws.com/images/experiment.png" alt=""> </div> <h4>Reagent</h4> <p> <a href="https://www.peeref.com/works/33999889/resource" class="btn btn-outline-primary btn-sm"> Community support </a> </p> </div> </div> </div> </div> <div class="col-md-8 px-0 pl-md-3"> <div id="article-summary-panel" class="mb-4"> <ul class="nav nav-tabs" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#ai_summary" data-toggle="tab" class="summary-tab mx-0 f16 text-dark"> <strong>Automated Summary</strong> <strong class="text-danger ml-1"><i>New</i></strong> </a> </li> <li class=""> <a href="#raw_abstract" data-toggle="tab" class="abstract-tab mx-0 f16 text-dark"> <strong>Abstract</strong> </a> </li> </ul> <div class="tab-content border border-top-0"> <div id="ai_summary" class="tab-pane active"> <div class="summary-panel panel-box mb-0 rounded shadow-none"> <div class="f16">A dual-mode sensing platform based on CRISPR-Cas12a technology has been developed for the detection of OTA in Chinese herbs. The platform shows high sensitivity and good anti-interference ability against complex substrates.</div> </div> </div> <div id="raw_abstract" class="tab-pane "> <div class="abstract-panel panel-box mb-0 rounded shadow-none"> <div class="f16">Many traditional Chinese herbs are susceptible to ochratoxin A (OTA), a potent mycotoxin, which causes serious effects on the quality of the herb and on people's health. The development of methods to detect OTA is extremely important. Most methods for detecting OTA are based on a single-signal output mode, which might be easily influenced by complex environmental conditions. In this research, by taking advantage of the cleavage of DNA by target-induced CRISPR-Cas12a activity and the difference in electrostatic force of DNA to different charge electrochemiluminescent (ECL) and electrochemical (EC) probes, a biosensor is developed for the detection of OTA. First, the CRISPR-Cas12a system consists of a well-designed crRNA, its complementary strand (also as an aptamer for OTA), and Cas12a. Without the target, this CRISPR-Cas12a system is in the activated stage, which digests hairpin DNA on the electrode, resulting in a weak ECL signal and strong current response. With the introduction of OTA bound with the aptamer, CRISPR-Cas12a activity is inhibited (locked stage). Thus, hairpin DNA remained intact on the electrode, resulting in recovery of the ECL signal and attenuation of the current intensity. As a result, this label-free dual-mode sensing platform realizes an assay for OTA in Morinda officinalis. This target-regulated CRISPR-Cas12a activity-sensing platform with dual-mode output not only provides high sensitivity (due to the CRISPR-Cas12a system), but also has good anti-interference ability against complex substrates (due to dual-mode output), and exhibits a broad range of prospects for application.</div> </div> </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Authors</h4> <div class="mb-3"> <article-authors tid="33999889" list="[{"name":"Huifeng Xu","sequence":1},{"name":"Rui Pan","sequence":2},{"name":"Weihua Huang","sequence":3},{"name":"Xi Zhu","sequence":4}]" verified="[]" page="work" ></article-authors> </div> <div class="alert alert-warning mb-0"> <h5 class="mt-0 bg-warning text-dark px-3 rounded d-inline-block"> I am an author on this paper </h5> <div class="font-weight-bold f13"> Click your name to claim this paper and add it to your profile. </div> </div> </div> <div class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Reviews</h4> <div class="d-flex flex-wrap flex-md-nowrap"> <div class="flex-grow-1"> <h4 class="f16"> Primary Rating <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="The primary rating indicates the level of overall quality for the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap align-items-center alert mb-0"> <div class="d-flex align-items-center justify-content-center"> <Rate disabled allow-half value="4.6" style="font-size: 28px;"></Rate> <strong class="f20 m-3" style="color: #f5a623;">4.6</strong> </div> <div class="text-muted mx-4"> Not enough ratings </div> </div> <h4 class="f16"> Secondary Ratings <a href="javascript:;" data-toggle="tooltip" data-placement="right" title="Secondary ratings independently reflect strengths or weaknesses of the paper."> <i class="ivu-icon ivu-icon-md-help-circle f18 ml-2"></i> </a> </h4> <div class="d-flex flex-wrap flex-md-nowrap alert"> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Novelty</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Significance</h5> <strong class="mx-4">-</strong> </div> <div class="d-flex flex-shrink-0 align-items-center mr-3"> <h5 class="my-0">Scientific rigor</h5> <strong class="mx-4">-</strong> </div> </div> </div> <div class="flex-shrink-0"> <div class="border bg-light py-2 px-4"> <h5 class="mb-1">Rate this paper</h5> <Rate class="f24" @on-change="function(value){ location.href='https://www.peeref.com/works/33999889/comments?rating='+value }"></Rate> </div> </div> </div> </div> <div id="collection" class="f15 panel-box rounded shadow-none border"> <h4 class="mt-0 heading-count">Recommended</h4> <div class="my-3"> <ul class="nav nav-pills border-bottom pb-3" style="list-style: none; padding-left: 0;"> <li class="active"> <a href="#articles_from_related" data-toggle="tab" class="mx-0 f15"> <strong>Related</strong> </a> </li> <li class=""> <a href="#articles_from_authors" data-toggle="tab" class="mx-0 f15"> <strong>From Same Authors</strong> </a> </li> <li class=""> <a href="#articles_from_journal" data-toggle="tab" class="mx-0 f15"> <strong>From Same Journal</strong> </a> </li> </ul> <div class="tab-content"> <div id="articles_from_related" class="tab-pane active"> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/33999546" class="text-dark hover-underline">Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis</a> </h4> <p class="text-ellipsis-2">Huifeng Xu, Rui Pan, Weihua Huang, Xi Zhu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/520.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a biosensor for the detection of ochratoxin A (OTA) in traditional Chinese herbs was developed using the CRISPR-Cas12a system and electrochemical probes. The biosensor not only showed high sensitivity, but also demonstrated good anti-interference ability against complex substrates, providing promising prospects for application. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/33999546/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/26745742" class="text-dark hover-underline">A novel label-free universal biosensing platform based on CRISPR/Cas12a for biomarker detection</a> </h4> <p class="text-ellipsis-2">Xiaomei Mu, Xin Wang, Yuxin Qin, Yong Huang, Jianniao Tian, Shulin Zhao</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7745.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> We developed a biosensing platform using CRISPR/Cas system and a fluorescent label-free biosensor for sensitive and specific detection of disease-related biomarkers. The system utilizes an exonuclease III-mediated target cycle and a universal trigger chain to amplify the signal. The platform can be easily transformed to detect proteins, small molecules, or microRNA in biological samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">TALANTA</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26745742/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Multidisciplinary </span> </div> <h4> <a href="https://www.peeref.com/works/83553634" class="text-dark hover-underline">Label-Free and Universal CRISPR/Cas12a-Based Detection Platform for Nucleic Acid Biomarkers</a> </h4> <p class="text-ellipsis-2">Rujian Zhao, Yao Xiao, Yidan Tang, Baiyang Lu, Bingling Li</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/10311.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study developed a rapid, universal, and label-free CRISPR/Cas12a-based biomarker detection platform by designing a G4-containing hairpin structure as the reporter, which improved the detection efficiency and signal strength, eliminated the need for complex modifications, and could detect various biomarkers, achieving label-free detection in clinical samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ACS SENSORS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83553634/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/28585377" class="text-dark hover-underline">Construction of a fluorescence sensing platform based on RISC and CRISPR/ Cas12a for the assay of Ago2 enzyme activity</a> </h4> <p class="text-ellipsis-2">Xiaoqing Wen, Chenyi Zhuo, Jihua Wei, Yuanxun Gong, Qianli Tang, Xianjiu Liao, Kai Zhang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/5871.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study successfully constructed a fluorescence sensing platform for the detection of endogenous Ago2 activity in complex samples. The method has the advantages of high specificity, sensitive detection, easy operation, and low instrumentation requirements. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">MICROCHEMICAL JOURNAL</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/28585377/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biochemistry & Molecular Biology </span> </div> <h4> <a href="https://www.peeref.com/works/81959906" class="text-dark hover-underline">An optimized microRNA detection platform based on PAM formation-regulated CRISPR/Cas12a activation</a> </h4> <p class="text-ellipsis-2">Dawei Li, Pengda Liang, Shen Ling, Yapeng Wu, Bei Lv</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/3674.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study compared two design strategies of hairpin DNA in HCR and developed a miRNA detection platform that can identify different miRNAs by replacing the loop region of the probe, and can also detect single-stranded DNA and base mutations. The platform can discriminate the expression levels of miRNA in different cell lines, and has potential applications in the early diagnosis of cancers. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81959906/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biophysics </span> </div> <h4> <a href="https://www.peeref.com/works/81934530" class="text-dark hover-underline">Dual toeholds regulated CRISPR-Cas12a sensing platform for ApoE single nucleotide polymorphisms genotyping</a> </h4> <p class="text-ellipsis-2">Yuedong Zhu, Yanan Lin, Bin Gong, Yan Zhang, Gaoxing Su, Yanyan Yu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1203.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A universal and high-fidelity genotyping platform based on dual toeholds regulated Cas12a sensing methodology was established, which can be used in pharmacogenomics and diagnostics. This platform overcomes the PAM limitation through cascade toehold mediated strand displacement reaction, and has high universality and ultra-specificity. To improve the sensitivity, an improved isothermal recombinant polymerase amplification (RPA) strategy was developed, and visual readout of genotyping results was achieved by using commercial lateral flow strips. This platform can accurately discriminate single nucleotide polymorphisms of ApoE gene in human buccal swab samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOSENSORS & BIOELECTRONICS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81934530/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/81945383" class="text-dark hover-underline">Label-free electrochemical sensing platform for sensitive detection of ampicillin by combining nucleic acid isothermal enzyme-free amplification circuits with CRISPR/Cas12a</a> </h4> <p class="text-ellipsis-2">Li Zhu, Xuemei Zhang, Li Yang, Shan Qiu, Guoyu Liu, Xiaoli Xiong, Ting Xiao, Ke Huang, Liping Zhu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7745.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A label-free electrochemical sensor based on NH2-Co-MOF was developed for the detection of AMP in food and environment. The sensor combines HCR, CHA, and CRISPR/Cas12a techniques, showing high sensitivity and specificity. It performed well in real sample detection and has broad application prospects. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">TALANTA</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81945383/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/81817559" class="text-dark hover-underline">Label/immobilization-free Cas12a-based electrochemiluminescence biosensor for sensitive DNA detection</a> </h4> <p class="text-ellipsis-2">Honghong Wang, Xiaomin Hang, Huiyi Wang, Jiaxin Peng, Haoming Yu, Li Wang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7745.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study achieved a simple, rapid, and label/modification-free detection of target HPV-16 by encapsulating ECL luminophores carbon dots in situ in DNA hydrogels through a simple rolling circle amplification reaction. The method can detect 0.63 pM HPV-16 DNA without any amplification steps and only takes about 60 minutes to quickly test human serum samples, showing great potential in point-of-care testing. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">TALANTA</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/81817559/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/26071356" class="text-dark hover-underline">A CRISPR/Cas12a-based label-free fluorescent method for visual signal output</a> </h4> <p class="text-ellipsis-2">Liu Wang, Fang He, Xueyun Chen, Kaiyu He, Linlin Bai, Qiang Wang, Fang Zhang, Xiahong Xu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7475.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR/Cas12a-based label-free fluorescent visual detection strategy is proposed, utilizing G-quadruplex to enhance fluorescent emission and Cas12a to disrupt higher-ordered structure. The method shows high sensitivity and specificity in detecting Vibrio parahaemolyticus and requires less sophisticated equipment compared to real-time PCR. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SENSORS AND ACTUATORS B-CHEMICAL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26071356/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/75176109" class="text-dark hover-underline">A CRISPR/Cas12a-based label-free fluorescent method for visual signal output</a> </h4> <p class="text-ellipsis-2">Liu Wang, Fang He, Xueyun Chen, Kaiyu He, Linlin Bai, Qiang Wang, Fang Zhang, Xiahong Xu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7475.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> CRISPR/Cas12a-based label-free fluorescent visual detection strategy was proposed in this research, utilizing G-quadruplex to enhance fluorescent emission and Cas12a to disrupt the structured formation for visual observation. With the combination of Thioflavin T and loop-mediated isothermal amplification, the method showed high sensitivity and specificity in detecting Vibrio parahaemolyticus, as well as distinguishing Salmo salar from other similar species in the Salmonidae family. The findings will expand the application of CRISPR/Cas12a-based label-free fluorescent analysis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SENSORS AND ACTUATORS B-CHEMICAL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/75176109/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Biophysics </span> </div> <h4> <a href="https://www.peeref.com/works/26422849" class="text-dark hover-underline">A versatile CRISPR/Cas12a-based biosensing platform coupled with a target-protected transcription strategy</a> </h4> <p class="text-ellipsis-2">Xinyue Kang, Chao Lei, Jingjing Shi, Xiaoling Liu, Wei Ren, Chenghui Liu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/1203.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study develops a versatile CRISPR/Cas12a sensing platform by combining a target protection-based transcription amplification strategy with the Cas12a-based signal amplification mechanism. This platform enables the sensitive detection of both nucleic acid and non-nucleic acid targets. The new strategy eliminates non-specific transcription background and broadens the application scenarios of CRISPR/Cas system in the field of bioanalysis and biosensing. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">BIOSENSORS & BIOELECTRONICS</span> (2023) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/26422849/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/84320263" class="text-dark hover-underline">Dual mature microRNA-responsive logic biosensing platform based on CRISPR/Cas12a and DNA nanocage</a> </h4> <p class="text-ellipsis-2">Mei Wen, Zhaoxin Huang, Yao Yin, Yin Wang, Menghui Wang, Xueyuan Huang, Ting Chen, Guoliang Ke, Mei Chen, Xiao-Bing Zhang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7745.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study constructed a dual mature microRNA-responsive second-order logic biosensing platform for accurate cancer diagnosis. The platform achieved size-selective discrimination of mature microRNAs and pre-microRNAs and cooperative triggering of trans-cleavage by dual activators through DNA nanocages and CRISPR/Cas12a system, with high sensitivity and accuracy. This study provides a new idea for the development of intelligent biosensors, which is expected to be widely used in biological science research and clinical disease diagnosis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">TALANTA</span> (2025) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84320263/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/84693377" class="text-dark hover-underline">A nanopore-based label-free CRISPR/Cas12a system for portable and ultrasensitive detection of zearalenone</a> </h4> <p class="text-ellipsis-2">Ziye Pei, Zhuoqun Su, Jianing Chen, Wenrui Li, Di Wu, Lin Li, Yongning Wu, Guoliang Li</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/514.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study constructed a nanopore-based label-free CRISPR/Cas12a system for the detection of ZEN in food. The system has the advantages of low detection limit, rapidity, portability, and on-site detection potential. The verification results in real corn samples show that the method has good recovery and low relative standard deviation. This method provides a new idea and platform for the detection of ZEN and other mycotoxins. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICA CHIMICA ACTA</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84693377/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/24853212" class="text-dark hover-underline">P53-MDM2 interaction monitoring and inhibitors potency evaluation based on CRISPR-Cas12a sensing platform</a> </h4> <p class="text-ellipsis-2">Min Zhu, Jianbin Pan, Mengting Xu, Jiajia Cao, Yuedong Zhu, Hongyan Zhu, Gaoxing Su, Yanyan Yu</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/7475.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a protein-protein interaction (PPIs) detection platform based on the CRISPRCas12a sensing system was developed for accurate monitoring of p53-MDM2 interaction and evaluation of inhibitors potency. The proposed method utilizes the conformational-dependent DNA binding ability of p53, incorporating it into the Cas12a-mediated collateral cleavage activity to transduce the PPI event. It shows great potential in PPIs monitoring and inhibitors potency evaluations. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">SENSORS AND ACTUATORS B-CHEMICAL</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/24853212/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/25769579" class="text-dark hover-underline">AND Logic-Gate-Based CRISPR/Cas12a Biosensing Platform for the Sensitive Colorimetric Detection of Dual miRNAs</a> </h4> <p class="text-ellipsis-2">Shaohua Gong, Xi Wang, Ping Zhou, Wei Pan, Na Li, Bo Tang</p> <div class="d-flex mb-3"> <div class="flex-shrink-0 d-none d-sm-block"> <img src="https://peeref-open.s3.amazonaws.com/storage/images/covers/518.jpg" alt="" class="border mr-3" width="100"> </div> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> In this study, a CRISPRCas12a-based biosensing platform was developed for sensitive colorimetric detection of dual miRNAs. The method allows direct differentiation by the naked eye and detection of miRNA in real human serum, providing potential for precise disease diagnosis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL CHEMISTRY</span> (2022) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/25769579/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> <div id="articles_from_authors" class="tab-pane "> <div class="nodata my-4">No Data Available</div> </div> <div id="articles_from_journal" class="tab-pane "> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83020778" class="text-dark hover-underline">Recent progress on DNAzyme-based biosensors for pathogen detection</a> </h4> <p class="text-ellipsis-2">Xingxing Liu, Wenxu Yuan, Heng Xiao</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This article reviews the application of DNAzymes in pathogen detection, focusing on two main types of DNAzymes, HGD and RCD, and their research progress in pathogen detection. In addition, two novel DNAzymes, CLICK 17 DNAzyme and Supernova DNAzyme, are introduced, which show great potential in pathogen detection. Finally, the advantages and disadvantages of these four DNAzymes are summarized, and feasible suggestions for the development of biosensors are provided. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83020778/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/82994504" class="text-dark hover-underline">Photonic crystal gas sensors based on metal-organic frameworks and polymers</a> </h4> <p class="text-ellipsis-2">Jianan Wei, Zhihao Yi, Liu Yang, Ling Zhang, Junchao Yang, Molin Qin, Shuya Cao</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This review presents the research progress of PC gas sensors based on MOFs and polymers. Firstly, the optical properties and gas sensing mechanism of PCs are briefly described, then the structural properties and preparation methods of DBRs, opals, and IOPCs are discussed in detail. Next, the recent advances in MOF, polymer, and MOF/polymer-based PC sensors are summarized, including strategies for enhancing sensitivity and selectivity. Finally, the prospects of PC gas sensors are discussed in terms of preparation methods, material functionalization, and future applications. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82994504/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83693564" class="text-dark hover-underline">A label-free electrochemical biosensor based on a bimetallic organic framework for the detection of carbohydrate antigen 19-9</a> </h4> <p class="text-ellipsis-2">Tongxiao Zhao, Baokang Jin</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A novel electrochemical immunosensor based on rGO@Ce-MOF-on-Fe-MOF@TB nanocomposites was constructed for sensitive detection of CA19-9, achieving a wide linear range (1-100 000 mU mL-1) and a low detection limit (0.34 mU mL-1). </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83693564/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83037987" class="text-dark hover-underline">A novel dual-model photoelectrochemical/electrochemical sensor based on Z-scheme TiO2 disks/methylene blue for kanamycin detection</a> </h4> <p class="text-ellipsis-2">Wenchao Geng, Huimin Liu, Zhiyi Yan, Jiangying Ji, Fei Wang, Ruiying Yang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A dual-mode sensor for kanamycin detection was developed based on Z-scheme TiO2 disk/MB sensitization. The sensor showed high sensitivity and selectivity, and could be applied for the rapid determination of antibiotics in food. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83037987/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83135840" class="text-dark hover-underline">A magnetic plasma Fe3O4@Cu@Cu2O photoelectrochemical sensor for the detection of fumonisin B1</a> </h4> <p class="text-ellipsis-2">Xiaodong Xue, Yanqiu Leng, Yanmei Li, Jinhuan Zhang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A Fe3O4@Cu@Cu2O plasma nanocomposite was synthesized as a sensing platform for the detection of fumonisin B1. The sensor has a wide linear range and a low detection limit, and can realize the specific detection of FB1 in real samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83135840/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83002619" class="text-dark hover-underline">Comprehensive assessment of clean-up strategies for optimizing an analytical multi-method to determine pesticides and mycotoxins in Brazilian medicinal herbs using QuEChERS-LC-TQ-MS/MS</a> </h4> <p class="text-ellipsis-2">Marlos Eduardo Zorzella Fontana, Rosselei Caiel da Silva, Ingrid Duarte dos Santos, Julia Paula Neu, Robson Dias Wouters, Paola Jennifer Babinski, Jessica Fernanda Hoffmann, Rochele Cassanta Rossi, Liliana Essi, Ionara Regina Pizzutti</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A sensitive, accurate, precise, and effective QuEChERS method was developed and optimized for the simultaneous determination of over 160 pesticide and mycotoxin residues in complex medicinal plant matrices using LC-TQ-MS/MS. The method was validated and can be used for routine monitoring, as well as providing a basis for further research on risk assessment and control in food safety. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83002619/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83157007" class="text-dark hover-underline">Detection and identification of SARS-CoV-2 and influenza a based on microfluidic technology</a> </h4> <p class="text-ellipsis-2">Yujie Liu, Guanliu Yu, Hongkun Liang, Wenbo Sun, Lulu Zhang, Michael G. Mauk, Hua Li, Lei Chen</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This system combines RPA and CRISPR assays for rapid, highly specific, and sensitive detection and differentiation of three viruses. Under isothermal amplification conditions, the detection limit is as low as 5 copies per microliter, and the measurement time is approximately 30 minutes. The combination of this system with a microfluidic chip allows for simultaneous detection of three viruses, providing a potential solution for efficient and reliable diagnosis. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83157007/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83227752" class="text-dark hover-underline">A novel chemiluminescence sensor for alpha-fetoprotein detection based on an aptamer-luminol modified magnetic graphene oxide and copper-based MOF composite</a> </h4> <p class="text-ellipsis-2">Juntao Liu, Xueying Wang, Yuanling Sun, Chuannan Luo</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A novel target-triggered turn-on chemiluminescence sensor was fabricated for the detection of alpha-fetoprotein. The sensor was based on an aptamer-luminol modified magnetic graphene oxide and copper-based MOF composite, which exhibited high selectivity and sensitivity. The linear range of the sensor was 1.0 × 10-4-50 ng mL-1, and the detection limit was 4.2 × 10-5 ng mL-1. The sensor also showed good selectivity, reproducibility, and stability, and was successfully applied to the detection of AFP in serum samples. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83227752/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/82931426" class="text-dark hover-underline">Functional fibers/textiles for smart sensing devices and applications in personal healthcare systems</a> </h4> <p class="text-ellipsis-2">Wangdong Lu, Guoxin Wu, Linli Gan, Yingying Zhang, Kai Li</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This review focuses on the recent advances in smart fiber/textile-based sensing devices for wearable electronic applications, including fabrication strategies, sensing performances, working principles, and applications. It also proposes current challenges and future prospects, emphasizing the importance of keeping up with the sequential exploration of soft sensing fibers/textiles for applications in wearable smart systems. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/82931426/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/84362109" class="text-dark hover-underline">Gas chromatography-ion mobility spectrometry for the detection of human disease: a review</a> </h4> <p class="text-ellipsis-2">Li Gao, Ruiwen Yang, Jizhou Zhang, Miaomiao Sheng, Yun Sun, Bing Han, Guoyin Kai</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> GC-IMS can detect volatile organic compounds in various human samples for the diagnosis of multiple diseases. Some disease markers have been identified, providing a non-invasive method for the monitoring and diagnosis of human diseases with broad application prospects. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84362109/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83735621" class="text-dark hover-underline">Fluorescence and colorimetric analysis of β-estradiol based on aptamer assembled spherical nucleic acids</a> </h4> <p class="text-ellipsis-2">Leyuan Chen, Aijiao Yuan, Dapeng Zhang, Wenjing Xie, Hanyong Peng</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A novel biosensing strategy using E2 aptamer assembled spherical nucleic acids has been developed for the detection of β-estradiol in the environment. The method combines the sensitivity of fluorescence and the simplicity of colorimetry, and can be performed on-site without the need for expensive instrumentation. The method has been successfully applied to the determination of E2 concentrations in tap water and serum samples, and shows high selectivity towards E2 over other estrogens. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83735621/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/84062476" class="text-dark hover-underline">A new filtration system for extraction and accurate quantification of microplastics</a> </h4> <p class="text-ellipsis-2">Juhye Kim, Jihye Kwon, JeongHyun Kwon, Muhammad Zain Siddiqui, Gijeong Woo, Mijung Choi, Sungguan Hong, Chuan Ma, Shogo Kumagai, Atsushi Watanabe, Norio Teramae, Su Shiung Lam, Young-Min Kim</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> A novel filtration device is presented to enhance the recovery efficiency of microplastics from liquid samples. The device addresses the issue of low recovery in traditional filtration systems due to the adsorption of microplastics on the glassware surface. Tests with fluorescent polyethylene particles show a significant increase in recovery with the new device. The study emphasizes the importance of filtration processing in microplastic analysis and proposes the new device as an advanced filtration system. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84062476/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Review </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/83505452" class="text-dark hover-underline">Current trends in colorimetric biosensors using nanozymes for detecting biotoxins (bacterial food toxins, mycotoxins, and marine toxins)</a> </h4> <p class="text-ellipsis-2">Li Feng, Mingcheng Zhang, Zhiyi Fan</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> Biotoxins are major threats in the fields of food, feed, and medicine, posing potential risks to human health. Nanozymes, as nanomaterials with enzyme-like activities, offer advantages such as customizable design, controlled catalytic performance, excellent biocompatibility, and superior stability. They have found wide application in the development of colorimetric biosensors. This review focuses on the recent progress in nanozyme research and their application in colorimetric biosensing of biotoxins, highlighting their characteristics and performance. The challenges and future prospects of these assays are also discussed. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/83505452/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 border-bottom"> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/84660941" class="text-dark hover-underline">A new pyranopyrazole based colorimetric chemosensor for the selective recognition of biothiols: applications in real samples</a> </h4> <p class="text-ellipsis-2">Swati Negi, Priya Takkar, Parveen Gahlyan, Rakesh Kumar</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This paper presents a pyranopyrazole-based chemosensor P1 for the detection of biothiols. The interaction of P1 with amino acids and biothiols was studied by colorimetry and spectroscopy. P1 shows a visible color change in the presence of biothiols and can detect them within seconds. It is effective in a wide pH range and can be used in solid-state and real sample analysis, including human plasma and commercial samples of Cys and GSH. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84660941/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> <div class="my-4 "> <div> <span class="d-inline-block badge badge-blue"> Article </span> <span class="d-inline-block badge badge-cyan"> Chemistry, Analytical </span> </div> <h4> <a href="https://www.peeref.com/works/84680267" class="text-dark hover-underline">A colorimetric strategy and smartphone-based test strip for the detection of glucose based on the peroxidase activity of a hemin-derived nanozyme</a> </h4> <p class="text-ellipsis-2">Ziqiang Yuan, Meizhen Fu, Xin Wang, Meng Wang, Yue Wei, Yanna Sun, Qingfeng Zhang, Yunyi Zhang, Bo Zhang</p> <div class="d-flex mb-3"> <div class="p-3 rounded bg-light-blue"> <strong>Summary:</strong> This study prepared a hemin-derived nanozyme, Hemin-CDs, which can be used as a substitute for natural horseradish peroxidase in colorimetric detection. The nanozyme has good performance and application prospects. It can be used to detect H2O2 and glucose with short detection time, wide linear range, low detection limit, and satisfactory detection results in human serum. In addition, a test strip based on a smart phone was developed, which can realize rapid and accurate glucose quantitative detection and has great application potential. </div> </div> <div class="d-flex justify-content-between"> <p class="font-weight-bold"> <span class="text-primary">ANALYTICAL METHODS</span> (2024) </p> <div class="flex-shrink-0"> <a class="btn btn-outline-primary btn-sm" href="https://www.peeref.com/works/84680267/add-to-collection" target="_blank"> <strong>Add to Collection</strong> </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="modal fade" id="export-citation" tabindex="-1"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <button type="button" class="close" data-dismiss="modal"><span>×</span></button> <h4 class="modal-title">Export Citation <b class="text-primary"></b></h4> </div> <div class="modal-body"> <div class="my-3 px-4 f16"> <form action="https://www.peeref.com/works/citation/download" 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Label-free dual-mode sensing platform based on target-regulated CRISPR-Cas12a activity for ochratoxin A in Morinda officinalis - Peeref