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chromatography with fluorescence detector for measuring tocopherols (α , β + γ and δ) has been developed. Oils are diluted in methanol: hexane: tetrahydrofuran (neither previous extraction of tocopherols nor saponification procedure are required) and after being vortexed and centrifuged, an aliquot of the overlay was injected directly into an Alltima C 18 column. Acetonitrile and methanol (50: 50) mixture was used as a mobile phase with a flow rate of 1 mL min-1. Quantification of tocopherols was performed by fluorescence detector at 290 nm excitation wavelength and 325 nm emission wavelength. Tocopherols were separated at 25°C in less than 10 min after injection. The method has good limit of detection (9 ng g-1 for α-tocopherol and 8 ng g-1 for β-, γ-and δ-tocopherols) and reproducibility (CV\u003c 2.9 %). This method can be used to assess the influence of genetic modification of oil seeds on the distribution of tocopherols or the effect of tocopherols on the oxidative stability of edible plant oils.","grobid_abstract_attachment_id":"76157345"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Tocopherol content in vegetable oils using a rapid HPLC fluorescence detection method","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [179288064]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div 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data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="34385558" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection">Analysis of tocopherols in vegetable oils by high-performance liquid chromatography: Comparison of fluorescence and evaporative light-scattering detection</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34694729" href="https://uga.academia.edu/wwwcaesugaedudepartmentsfstLBCAP">Casimir C Akoh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of the American Oil Chemists’ Society, 1994</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Analysis of tocopherols in vegetable oils by high-performance liquid chromatography: Comparison of fluorescence and evaporative light-scattering detection","attachmentId":54270951,"attachmentType":"pdf","work_url":"https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="40347682" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography">Extraction and quantification of tocopherols from edible oils using high performance liquid chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="126411885" href="https://salu.academia.edu/MSirohi">Muzafar H . Sirohi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Biosciences, 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">Adulteration of Extra Virgin Olive Oil with olive oil and sunflower oil remained major issue in edible oil industry. Herein, rapid, sensitive and precise method for the determination of all type of tocopherols in different vegetable oils includinhg sunflower oil, olive oil and extra virgin olive oil is reported. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with UV detector was used for tocopherol determination. A simple, quick and sensitive method to estimate the antioxidant quantity is proposed. In this method oils were diluted in methanol and injected directly into column (no saponification procedure). Methanol and water (98:2) mixture was used as mobile phase. Three tocopherols (α, γ and δ) were detected at 292 nm wavelength with UV detector. Method had good limit of detection (LOD) (7ng/g) and reproducibility (C.V% 0.9, 0.8 and 0.4 for α, γ and δ tocopherols, respectively). Result showed the best source for α-tocopherol was sun flower oil (146.65±1.7mg/kg). Oil richest in δ-tocopherol was olive oil and δ-tocopherol was absent in extra virgin olive oil. The current study suggest the new parameter (ratio of α/γ) as first screening indicator of authenticity of purity of extra virgin olive oil for differentiation of various cultivators of same generic source.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Extraction and quantification of tocopherols from edible oils using high performance liquid chromatography","attachmentId":60593264,"attachmentType":"pdf","work_url":"https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="120568914" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils">Development of an Advance Method for the Determination of Tocopherols in Edible Oils</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="160629219" href="https://independent.academia.edu/VandaFuss">Vanda Fuss</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Agricultura, 2020</p><p class="ds-related-work--abstract ds2-5-body-sm">We have developed a method for the determination of tocopherols in different edible oils by RP-HPLC, using a FLD detector, a Poroshell column as a stationary phase, and a mixture of acetonitrile, methanol and water (5:4.5:0.5) as mobile phase (flow rate 0.75 ml/min). The structure of the compounds was identical, so it could not be separated by reverse phase liquid chromatography. The obtained peaks show an important overlapping and we have used chemometric tools to get a calibration model, which assumes a linear behavior, i.e., the total signal is the sum of individual signals of components. Recovery percentages were between 90.3% and 95.7%. The method has been successfully applied to edible oils and dietary supplements. No pretreatment of the samples was needed.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development of an Advance Method for the Determination of Tocopherols in Edible Oils","attachmentId":115676043,"attachmentType":"pdf","work_url":"https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" 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Quantification of Tocopherols","attachmentId":39609060,"attachmentType":"pdf","work_url":"https://www.academia.edu/17605852/Synchronous_Fluorescence_Spectroscopy_of_Edible_Vegetable_Oils_Quantification_of_Tocopherols","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17605852/Synchronous_Fluorescence_Spectroscopy_of_Edible_Vegetable_Oils_Quantification_of_Tocopherols"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="78676861" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/78676861/Development_and_Validation_of_Simple_Reversed_Phase_Method_for_Separation_and_Quantification_of_Tocopherols_in_Edible_Oils">Development and Validation of Simple Reversed Phase Method for Separation and Quantification of Tocopherols in Edible Oils</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="131411281" href="https://independent.academia.edu/AdelShehata4">Adel Shehata</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Egyptian Journal of Chemistry, 2018</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development and Validation of Simple Reversed Phase Method for Separation and Quantification of Tocopherols in Edible Oils","attachmentId":85640981,"attachmentType":"pdf","work_url":"https://www.academia.edu/78676861/Development_and_Validation_of_Simple_Reversed_Phase_Method_for_Separation_and_Quantification_of_Tocopherols_in_Edible_Oils","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/78676861/Development_and_Validation_of_Simple_Reversed_Phase_Method_for_Separation_and_Quantification_of_Tocopherols_in_Edible_Oils"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="20465132" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/20465132/Tocopherol_speciation_as_first_screening_for_the_assessment_of_extra_virgin_olive_oil_quality_by_reversed_phase_high_performance_liquid_chromatography_fluorescence_detector">Tocopherol speciation as first screening for the assessment of extra virgin olive oil quality by reversed-phase high-performance liquid chromatography/fluorescence detector</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="42677838" href="https://independent.academia.edu/GiuseppeSalvetti">Giuseppe Salvetti</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="41892199" href="https://independent.academia.edu/MAngiuli">M. 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