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(PDF) Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems | Patricia Oliveira - Academia.edu

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"https://www.academia.edu/login?post_login_redirect_url=https%3A%2F%2Fwww.academia.edu%2F23202164%2FQuantification_of_Tocopherols_and_Tocotrienols_in_Portuguese_Olive_Oils_Using_HPLC_with_Three_Different_Detection_Systems%3Fshow_translation%3Dtrue"; window.loswp.previewableAttachments = [{"id":43690954,"identifier":"Attachment_43690954","shouldShowBulkDownload":false}]; window.loswp.shouldDetectTimezone = true; window.loswp.shouldShowBulkDownload = true; window.loswp.showSignupCaptcha = false window.loswp.willEdgeCache = false; window.loswp.work = {"work":{"id":23202164,"created_at":"2016-03-13T12:49:33.167-07:00","from_world_paper_id":150385845,"updated_at":"2024-11-15T22:19:29.844-08:00","_data":{"grobid_abstract":"Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: R-T, -T, γ-T, δ-T, R-T 3 , and γ-T 3 . R-Tocopherol (R-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.","publication_date":"2006,,","publication_name":"J Agr Food Chem","grobid_abstract_attachment_id":"43690954"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [45011450]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;swp-splash-paper-cover&quot;,&quot;attachmentId&quot;:43690954,&quot;attachmentType&quot;:&quot;pdf&quot;}"><img alt="First page of “Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/43690954/mini_magick20190215-31280-pxjhdr.png?1550274061" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="45011450" href="https://independent.academia.edu/PatriciaOliveira116"><img alt="Profile image of Patricia Oliveira" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Patricia Oliveira</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2006, J Agr Food Chem</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">6 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 23202164; 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Sirohi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Biosciences, 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">Adulteration of Extra Virgin Olive Oil with olive oil and sunflower oil remained major issue in edible oil industry. Herein, rapid, sensitive and precise method for the determination of all type of tocopherols in different vegetable oils includinhg sunflower oil, olive oil and extra virgin olive oil is reported. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with UV detector was used for tocopherol determination. A simple, quick and sensitive method to estimate the antioxidant quantity is proposed. In this method oils were diluted in methanol and injected directly into column (no saponification procedure). Methanol and water (98:2) mixture was used as mobile phase. Three tocopherols (α, γ and δ) were detected at 292 nm wavelength with UV detector. Method had good limit of detection (LOD) (7ng/g) and reproducibility (C.V% 0.9, 0.8 and 0.4 for α, γ and δ tocopherols, respectively). Result showed the best source for α-tocopherol was sun flower oil (146.65±1.7mg/kg). Oil richest in δ-tocopherol was olive oil and δ-tocopherol was absent in extra virgin olive oil. 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js-wsj-grid-card" data-collection-position="4" data-entity-id="63880756" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/63880756/Tocopherol_content_in_vegetable_oils_using_a_rapid_HPLC_fluorescence_detection_method">Tocopherol content in vegetable oils using a rapid HPLC fluorescence detection method</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="179288064" href="https://independent.academia.edu/RaducuCamelia">Camelia Raducu</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Tocopherol content in vegetable oils using a rapid HPLC fluorescence detection 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js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection">Analysis of tocopherols in vegetable oils by high-performance liquid chromatography: Comparison of fluorescence and evaporative light-scattering detection</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34694729" href="https://uga.academia.edu/wwwcaesugaedudepartmentsfstLBCAP">Casimir C Akoh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of the American Oil Chemists’ Society, 1994</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Analysis of tocopherols in vegetable 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style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="78480952" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/78480952/Tocopherols_Chemical_Structure_Bioactivity_and_Variability_in_Croatian_Virgin_Olive_Oils">Tocopherols: Chemical Structure, Bioactivity, and Variability in Croatian Virgin Olive Oils</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="51510246" href="https://independent.academia.edu/MajaSpika">Maja Jukic Spika</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Products from Olive Tree, 2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Tocopherols: Chemical Structure, Bioactivity, and Variability in Croatian Virgin Olive Oils&quot;,&quot;attachmentId&quot;:85514333,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/78480952/Tocopherols_Chemical_Structure_Bioactivity_and_Variability_in_Croatian_Virgin_Olive_Oils&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/78480952/Tocopherols_Chemical_Structure_Bioactivity_and_Variability_in_Croatian_Virgin_Olive_Oils"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="11938642" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/11938642/Identification_and_quantification_of_tocopherols_in_vegetable_oils_by_thin_layer_chromatography">Identification and quantification of tocopherols in vegetable oils by thin-layer chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="29585141" href="https://independent.academia.edu/ClaudiaCimpoiu">Claudia Cimpoiu</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="237394676" href="https://independent.academia.edu/CasoniDorina">Dorina Casoni</a></div><p class="ds-related-work--metadata ds2-5-body-xs">JPC-Journal of Planar …, 2008</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Identification and quantification of tocopherols in vegetable oils by thin-layer chromatography&quot;,&quot;attachmentId&quot;:46429353,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/11938642/Identification_and_quantification_of_tocopherols_in_vegetable_oils_by_thin_layer_chromatography&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/11938642/Identification_and_quantification_of_tocopherols_in_vegetable_oils_by_thin_layer_chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="91753582" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/91753582/Simultaneous_HPLC_Determination_of_Tocopherols_Carotenoids_and_Chlorophylls_for_Monitoring_Their_Effect_on_Virgin_Olive_Oil_Oxidation">Simultaneous HPLC Determination of Tocopherols, Carotenoids, and Chlorophylls for Monitoring Their Effect on Virgin Olive Oil Oxidation</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34579728" href="https://independent.academia.edu/MariaTsimidou">Maria Tsimidou</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Agricultural and Food Chemistry, 1998</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Simultaneous HPLC Determination of Tocopherols, Carotenoids, and Chlorophylls for Monitoring Their Effect on Virgin Olive Oil Oxidation&quot;,&quot;attachmentId&quot;:94950878,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/91753582/Simultaneous_HPLC_Determination_of_Tocopherols_Carotenoids_and_Chlorophylls_for_Monitoring_Their_Effect_on_Virgin_Olive_Oil_Oxidation&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/91753582/Simultaneous_HPLC_Determination_of_Tocopherols_Carotenoids_and_Chlorophylls_for_Monitoring_Their_Effect_on_Virgin_Olive_Oil_Oxidation"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="120568914" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils">Development of an Advance Method for the Determination of Tocopherols in Edible Oils</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="160629219" href="https://independent.academia.edu/VandaFuss">Vanda Fuss</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Agricultura, 2020</p><p class="ds-related-work--abstract ds2-5-body-sm">We have developed a method for the determination of tocopherols in different edible oils by RP-HPLC, using a FLD detector, a Poroshell column as a stationary phase, and a mixture of acetonitrile, methanol and water (5:4.5:0.5) as mobile phase (flow rate 0.75 ml/min). The structure of the compounds was identical, so it could not be separated by reverse phase liquid chromatography. The obtained peaks show an important overlapping and we have used chemometric tools to get a calibration model, which assumes a linear behavior, i.e., the total signal is the sum of individual signals of components. Recovery percentages were between 90.3% and 95.7%. The method has been successfully applied to edible oils and dietary supplements. No pretreatment of the samples was needed.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Development of an Advance Method for the Determination of Tocopherols in Edible Oils&quot;,&quot;attachmentId&quot;:115676043,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--sticky-ctas&quot;,&quot;attachmentId&quot;:43690954,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--sticky-ctas&quot;,&quot;attachmentId&quot;:43690954,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_43690954" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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Simó-alfonso</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33933893" href="https://independent.academia.edu/LorenzoCerretani">Lorenzo Cerretani</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Agricultural and Food Chemistry, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Determination of Tocopherols and Tocotrienols in Vegetable Oils by Nanoliquid Chromatography with Ultraviolet−Visible Detection Using a Silica Monolithic 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href="https://www.academia.edu/54985830/Simultaneous_Determination_of_Pigments_Tocopherols_and_Squalene_in_Greek_Olive_Oils_A_Study_of_the_Influence_of_Cultivation_and_Oil_Production_Parameters"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="8" data-entity-id="103692834" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/103692834/Total_Phenolic_Compounds_and_Tocopherols_Profiles_of_Seven_Olive_Oil_Varieties_Grown_in_the_South_West_of_Spain">Total Phenolic Compounds and Tocopherols Profiles of Seven Olive Oil Varieties Grown in the South-West of Spain</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="274557649" href="https://independent.academia.edu/JacintoDaniloAbimaelCedilloS%C3%A1nchez">Jacinto Danilo Abimael Cedillo Sánchez</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Oleo Science, 2014</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Total Phenolic Compounds and Tocopherols Profiles of Seven Olive Oil Varieties Grown in the South-West of Spain&quot;,&quot;attachmentId&quot;:103632340,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/103692834/Total_Phenolic_Compounds_and_Tocopherols_Profiles_of_Seven_Olive_Oil_Varieties_Grown_in_the_South_West_of_Spain&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span 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href="https://www.academia.edu/48590591/Chemometrics_for_the_resolution_of_co_eluting_peaks_of_%CE%B2_and_%CE%B3_tocopherols_in_RP_HPLC_Determination_in_edible_oils_and_dietary_supplements">Chemometrics for the resolution of co-eluting peaks of β- and γ-tocopherols in RP-HPLC: Determination in edible oils and dietary supplements</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="43383257" href="https://independent.academia.edu/TGaleanod%C3%ADaz">T. 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