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(PDF) Changes of Total Tocopherol and Tocopherol Species During Sunflower Oil Processing | saba naz - Academia.edu

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"https://www.academia.edu/login?post_login_redirect_url=https%3A%2F%2Fwww.academia.edu%2F1307638%2FChanges_of_Total_Tocopherol_and_Tocopherol_Species_During_Sunflower_Oil_Processing%3Fshow_translation%3Dtrue"; window.loswp.previewableAttachments = [{"id":51036270,"identifier":"Attachment_51036270","shouldShowBulkDownload":false}]; window.loswp.shouldDetectTimezone = true; window.loswp.shouldShowBulkDownload = true; window.loswp.showSignupCaptcha = false window.loswp.willEdgeCache = false; window.loswp.work = {"work":{"id":1307638,"created_at":"2012-01-29T00:35:31.795-08:00","from_world_paper_id":4996935,"updated_at":"2024-11-12T06:43:44.138-08:00","_data":{"publisher":"Springer","grobid_abstract":"The changes of total and individual tocopherols were investigated during different sunflower oil processing stages by reverse-phase high-performance liquid chromatography. The results revealed that the levels of total and individual tocopherol content were decreased during the neutralization, bleaching, and deodorization processes. The overall loss of total tocopherols during these stages was found to be 37.9%, although the general reduction trend of delta (d), gamma (c), and alpha (a) tocopherols is very similar during neutralization (35.3%), bleaching (38.3%), and deodorization (37.8%). However, in contrast to the neutralizing and deodorizing stages, the bleaching process caused relatively less reduction for individual tocopherol contents. Deodorizer distillates were also analyzed and were found to be rich with tocopherols content (29,348.24 lg/ml). The results of the study indicated that most parts of the tocopherols are wasted during processing. Therefore, the proper concentration of nutritionists, industrialists, and manufacturers is needed for the necessary improvements in processing technology to avoid the major loss of tocopherols and to increase the shelf life, as well as the nutritive value of processed oil.","publication_date":"2010,1,1","publication_name":"Journal of the American Oil Chemists' Society","grobid_abstract_attachment_id":"51036270"},"document_type":"paper","pre_hit_view_count_baseline":0,"quality":"high","language":"en","title":"Changes of Total Tocopherol and Tocopherol Species During Sunflower Oil Processing","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [413456]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; 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translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="2912679" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/2912679/Tocopherol_An_intrinsic_component_of_sunflower_seed_oil_bodies">Tocopherol—An intrinsic component of sunflower seed oil bodies</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="3425651" href="https://nottingham.academia.edu/IanFisk">Ian Fisk</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of The American Oil Chemists Society, 2006</p><p class="ds-related-work--abstract ds2-5-body-sm">Oil bodies were removed from mature sunflower through wet grinding followed by filtration then centrifugation and recovered as the buoyant fraction. Washing this fraction with buffer (water-washed oil bodies, WWOB) or 9 M urea (urea-washed oil bodies, UWOB) resulted in the removal of extraneous proteins. SDS-PAGE of the proteins still associated with the oil body fraction after washing indicated that this effect was particularly dramatic with urea washing. Thirty-eight percent of the total seed tocopherol was recovered in WWOB after only one cycle of oil body recovery. The total phenolic content (TPC) of differentially washed sunflower seed oil bodies was used as a marker for the nonspecific association of phenolic compounds to oil bodies. This value decreased with increased removal of proteins from oil bodies, whereas the converse was true for tocopherol values, which increased from 214 mg total tocopherol kg−1 WWOB [dry wt basis (dwb)] to 392 mg total tocopherol kg−1 UWOB (dwb). The ratio of the four tocopherol isomers remained constant in the seed and oil body preparations (α:β:γ:δ approximately 94∶5∶0.5∶0.5). This work provides evidence that an intrinsic population of tocopherol molecules exists in the oil bodies of mature sunflower seeds.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Tocopherol—An intrinsic component of sunflower seed oil bodies&quot;,&quot;attachmentId&quot;:30845946,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/2912679/Tocopherol_An_intrinsic_component_of_sunflower_seed_oil_bodies&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/2912679/Tocopherol_An_intrinsic_component_of_sunflower_seed_oil_bodies"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="40347682" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography">Extraction and quantification of tocopherols from edible oils using high performance liquid chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="126411885" href="https://salu.academia.edu/MSirohi">Muzafar H . Sirohi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Biosciences, 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">Adulteration of Extra Virgin Olive Oil with olive oil and sunflower oil remained major issue in edible oil industry. Herein, rapid, sensitive and precise method for the determination of all type of tocopherols in different vegetable oils includinhg sunflower oil, olive oil and extra virgin olive oil is reported. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with UV detector was used for tocopherol determination. A simple, quick and sensitive method to estimate the antioxidant quantity is proposed. In this method oils were diluted in methanol and injected directly into column (no saponification procedure). Methanol and water (98:2) mixture was used as mobile phase. Three tocopherols (α, γ and δ) were detected at 292 nm wavelength with UV detector. Method had good limit of detection (LOD) (7ng/g) and reproducibility (C.V% 0.9, 0.8 and 0.4 for α, γ and δ tocopherols, respectively). Result showed the best source for α-tocopherol was sun flower oil (146.65±1.7mg/kg). Oil richest in δ-tocopherol was olive oil and δ-tocopherol was absent in extra virgin olive oil. The current study suggest the new parameter (ratio of α/γ) as first screening indicator of authenticity of purity of extra virgin olive oil for differentiation of various cultivators of same generic source.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Extraction and quantification of tocopherols from edible oils using high performance liquid chromatography&quot;,&quot;attachmentId&quot;:60593264,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="28870977" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/28870977/Comparison_of_Two_Different_Strategies_for_Tocopherols_Enrichment_Using_a_Molecular_Distillation_Process">Comparison of Two Different Strategies for Tocopherols Enrichment Using a Molecular Distillation Process</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="54359212" href="https://independent.academia.edu/MacielRubens">Rubens M Maciel Filho</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Industrial &amp; Engineering Chemistry Research, 2006</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;wsj-grid-card-download-pdf-modal&quot;,&quot;work_title&quot;:&quot;Comparison of Two Different Strategies for Tocopherols Enrichment Using a Molecular Distillation Process&quot;,&quot;attachmentId&quot;:49298215,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;work_url&quot;:&quot;https://www.academia.edu/28870977/Comparison_of_Two_Different_Strategies_for_Tocopherols_Enrichment_Using_a_Molecular_Distillation_Process&quot;,&quot;alternativeTracking&quot;:true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/28870977/Comparison_of_Two_Different_Strategies_for_Tocopherols_Enrichment_Using_a_Molecular_Distillation_Process"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;continue-reading-button--sticky-ctas&quot;,&quot;attachmentId&quot;:51036270,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{&quot;location&quot;:&quot;download-pdf-button--sticky-ctas&quot;,&quot;attachmentId&quot;:51036270,&quot;attachmentType&quot;:&quot;pdf&quot;,&quot;workUrl&quot;:null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_51036270" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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