Metagenomic Sequencing | GENEWIZ from Azenta

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understand the role of constituent species. Our metagenomic sequencing solutions range from targeted assays, such as 16S for prokaryotes and ITS2 for fungi, to shotgun sequencing and metagenomic analysis. Explore below which service best fits your needs, or check out our <a rel="noopener noreferrer" rel="noopener noreferrer" href="http://web.genewiz.com/microbial-diversity" target="_blank">webinar</a>, “Uncovering Hidden Microbial Diversity with NGS Technology,” to learn more about our capabilities.</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry?utm_source=metagenomics" target="_blank"><button>Request Quote</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/webinar/biological-mechanisms-predict-evolution-antibiotic-resistance?utm_campaign=2022-06 GEN NGS - New Customer Growth&utm_medium=web&utm_source=thumbnail&utm_medium=Metagenomics" target="_blank"><img alt="" src="/-/media/Images/13064-D_Lagator-Customer-Webinar_WebBanRight-(2).ashx?la=en&hash=1B3B247467F40C2326381D4DDF57F91C331CF8B6" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is metagenomic sequencing?</h2> <p>Metagenomic sequencing is the collective analysis of all genomic material present in a sample (containing mixed microorganism communities), thus eliminating the need for isolating and cultivating individual organisms.</p> <p> </p> <h2>What is metagenomics vs. genomics?</h2> <p>Both metagenomics and genomics are fields of study within molecular biology, but the difference is in scope. While genomics is focused on the genome of a single organism, metagenomics encompasses all genomes of various microorganisms within a single given sample, providing a broad overview of an ecosystem’s composition.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <p> </p> <style> .table-bordered tbody tr td { vertical-align: middle; } </style> <div class="container"> <h2>Metagenome Sequencing Service Options</h2> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td> </td> <td style="text-align: center;"><strong>16S-EZ</strong></td> <td style="text-align: center;"><strong>Fungal Identification</strong></td> <td style="text-align: center;"><strong>Whole Genome Metagenomics</strong></td> </tr> <tr> <td> <p style="text-align: center;"> <strong>Microbes Targeted</strong></p> </td> <td style="text-align: center;">Bacteria and Archaea</td> <td style="text-align: center;">Fungi</td> <td style="text-align: center;">All</td> </tr> <tr> <td> <p style="text-align: center;"><strong> Taxon Level</strong></p> </td> <td style="text-align: center;">Genus/Species</td> <td style="text-align: center;">Genus/Species</td> <td style="text-align: center;">Sub-species</td> </tr> <tr> <td style="text-align: center;"><strong>Genomic Loci Sequenced</strong></td> <td style="text-align: center;">16S rRNA gene<br /> (V3 and V4 regions)</td> <td style="text-align: center;">Internal Transcribed<br /> Spacer 2 (ITS2)</td> <td style="text-align: center;">Whole Genomes</td> </tr> <tr> <td style="text-align: center;"><strong>Purpose of Assay</strong></td> <td colspan="2" style="text-align: center;"> Taxon Identification</td> <td> <ul style="width: 70%; margin: auto;"> <li style="text-align: left;">Taxon identification</li> <li style="text-align: left;">Variant discovery</li> </ul> </td> </tr> <tr> <td style="text-align: center;"><strong>Benefits</strong></td> <td style="text-align: center;">Enhanced sensitivity outperforms<br /> V4-only assays</td> <td style="text-align: center;">High variation in ITS2<br /> region allows differentiation<br /> between closely related species</td> <td> <ul style="width: 70%; margin: auto;"> <li style="text-align: left;">Most comprehensive assay - identify over 53,000 microbes</li> <li style="text-align: left;">Phylogenetic analysis</li> <li style="text-align: left;">Ideal for organisms with poorly annotated 16S genes</li> </ul> </td> </tr> <tr> <td style="text-align: center;"><strong>Available Reports</strong></td> <td colspan="2"> <ul style="width: 70%; margin: auto;"> <li style="text-align: left;">Rank-abundance curve</li> <li style="text-align: left;">Taxonomic classification</li> <li style="text-align: left;">Rarefaction curve</li> <li style="text-align: left;">Principal coordinate analysis</li> <li style="text-align: left;">Hierarchical clustering</li> </ul> </td> <td> <ul style="width: 70%; margin: auto;"> <li style="text-align: left;">Taxonomic classification of reads across all ranks</li> <li style="text-align: left;">Microbial abundance estimation across all ranks</li> <li style="text-align: left;">Cross-sample comparisons across all ranks</li> <li style="text-align: left;">Dynamic, visual exploration of microbial taxonomy</li> <li style="text-align: left;">Interactive, searchable data tables</li> </ul> </td> </tr> </tbody> </table> </div> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner1 { width: 1170px; background-image: url('/-/media/11030DqPCRdPCRWebBanFullMedDesktopBackground.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner1 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner1-left { width: 70%; padding: 5px 0px 5px 20px; } #banner1-right { width: 20%; padding: 5px; } #banner1-right h1 { text-transform: none; 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} </style> <div class="container no-padding text-left"> <div class="row row-centered margin-top-40 margin-bottom-40"> <div class="col-md-12 col-centered"> <div class="service"> <span class="service-icon"><img alt="" style="height: 160px; width: 220px;" src="/-/media/Images/Services/NGS/photos/Microbial_Diversity_Thumbnail2.ashx?h=160&w=220&la=en&hash=95845DF62D671BAC433A77AD66755F68A57032CC" /></span> <div class="desc text-left"> <h4>Webinar: Uncovering Hidden Microbial Diversity with NGS Technology</h4> <p>This webinar outlines the role and importance of microorganisms in our everyday world, how to classify and identify them, and the next generation sequencing techniques that work best for these tasks. We touch on the wide array of areas that microbial genomics can be applied to and the NGS case studies that have helped scientists thrive in various research areas.</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="http://web.genewiz.com/microbial-diversity" target="_blank"><em class="fa icon fa-play icon-md"></em> <strong>Watch Now</strong></a> </div> </div> </div> <div class="col-md-12 col-centered"> <div class="service"><span class="service-icon"><img alt="Case Study" src="/-/media/Images/Services/NGS/photos/casestudy-thumbnail.ashx?h=160&w=220&la=en&hash=3216E258E5E15B9CACE88D314C44719E26FBED37" style="height: 160px; width: 220px;" /></span> <div class="desc text-left"> <h4>Tech Note: 16S MetaVx™ Environmental Detects Greater Prokaryotic Diversity Than Traditional 16S Assay</h4> <p>We compared GENEWIZ’s 16S MetaVx Environmental service against a traditional V4-only assay. 16S MetaVx Environmental consistently detected a greater number of bacteria and archaea, demonstrating its superior sensitivity and specificity.</p> <em class="fa icon fa-download icon-md"></em><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://3478602.fs1.hubspotusercontent-na1.net/hubfs/3478602/13047-CS 0322 16S MetaVX Case Study.pdf" target="_blank"> <strong>Download Now</strong></a></div> <div class="desc text-left"> </div> <div class="desc text-left">Please inquire for more details. 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Special focus is given on how these methods can be utilized to improve drug development and longevity.</p> <em class="fa icon fa-download icon-md"></em><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/webinar/biological-mechanisms-predict-evolution-antibiotic-resistance?utm_campaign=2022-06 GEN NGS - New Customer Growth&utm_medium=web&utm_source=thumbnail&utm_content=Metagenomics " target="_blank"> <strong>Watch Now</strong></a></div> <div class="desc text-left"> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container Component-BackGround-Default"> <h2>Sample Submission Guidelines</h2> <p>GENEWIZ accepts a variety of starting materials for Metagenomics projects. For detailed sample submission requirements please visit our <a href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines">Sample Submission Guidelines</a> page.</p> <p> </p> <h2>Deliverables</h2> <p>All customers receive their raw data as FASTQ files. MetaVx2.0™ provides an enhanced report through analysis with the QIIME software suite. This optional, enhanced report includes figures such as rank-abundance curves, taxonomic classification, rarefaction curves, principal coordinate analysis and hierarchical clustering analysis. An interactive taxonomic viewer detailing different taxa and their relative abundance in each sample is also available by request. </p> </div> <br> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Illumina"><img alt="Illumina" src="/-/media/hires-illumina-logo-rgb_new.ashx?w=200&la=en&hash=2DE7778E5877DA9F3D063A9FAF021DA575ECF223" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#PacBio"><img alt="PacBio" src="/-/media/PacBio-CSP_logo.ashx?w=200&la=en&hash=1568DC13B370C032463997ED7BC5591C1FECF672" 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