GENEWIZ from Azenta | AAV Plasmid Preparation

<!DOCTYPE html>    <html lang="en">  <head> <title>GENEWIZ from Azenta | AAV Plasmid Preparation</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="GENEWIZ AAV Plasmid Preparation service delivers superior quality for midi to giga-scale AAV vectors, while maintaining the integrity of ITR regions. 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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a 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style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='https://web.azenta.com/cell-and-gene-therapy' style='font-size:17px'>Cell and Gene Therapy Solutions 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} .aav-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .aav-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .aav-new-container { text-align: center; margin-top: 0; } .aav-new-content { width: 100%; margin: 0 auto; } .aav-new-content { display: inline-block; } #aav-content-box-right, #ngs-content-box-left { width: 100%; text-align: center; } #aav-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .aav-new-content { width: 100%; margin: 0 auto; } .aav-content-box img { width: 350px; } } </style> <div class="aav-new-container"> <div class="aav-new-content"> <div class="aav-content-box" id="aav-content-box-left"> <h1>AAV Plasmid Preparation</h1> <div> </div> <p>Preserving the integrity of inverted terminal repeat (ITR) sequences in adeno-associated virus (AAV) plasmids is important for the production of recombinant AAV (rAAV) vectors. Azenta Life Sciences, formerly GENEWIZ’s proprietary AAV plasmid preparation service delivers superior quality for midi to giga-scale AAV vectors, while maintaining the integrity of ITR regions. Our new process includes pre- and post-preparation quality controlled (QC) steps that leverage Azenta's <a href="/Public/Services/Sanger-Sequencing/AAV-ITR">AAV-ITR Sanger sequencing</a> capabilities for full sequence verification and ensures delivery of intact ITR clones for your research.</p> <br /> <a href="https://clims4.genewiz.com/DNAPrep?lobId=5&serviceId=530"><button>Place Order</button></a> </div> <div class="aav-content-box" id="aav-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/prep25?_ga=2.22954226.858825492.1654525039-1258591845.1617043252" target="_blank"><img alt="" src="/-/media/Images/15005D-Prep20OffPromoWebBanRightV1.ashx?la=en&hash=8698D8122942CE889C52D33DE6808F41725FDDAD" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner3-left { width: 50%; padding: 20px; } #banner3-right { width: 49%; padding: 10px; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 5; margin-bottom: 0px; color: #FFFFFF } #banner3-right h3 { text-transform: none; letter-spacing: 1px; line-height: 29px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h3 { text-transform: none; letter-spacing: 1px; line-height: 29px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #826ec3; border-radius: 3px; transition: all .2s ease-in-out; font-size: 18px; font-weight: bold; } #banner3-left button { padding: 7px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #826ec3; border-radius: 3px; transition: all .2s ease-in-out; font-size: 17px; font-weight: bold; } #banner3-right button:hover { cursor: pointer; transform: scale(1.1); } #banner3-left button:hover { cursor: pointer; transform: scale(1.1); } #banner3-right button:focus { outline: 0; } #banner3-left button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner3 { width: 95%; height: auto; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); display: inline-block; } #banner3-left { text-align: center; width: 100%; } #banner3-right { text-align: center; width: 100%; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner3-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner3-left img { width: auto; height: auto; } #banner3-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner3-left img { width: 60%; } #banner3-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner3"> <div id="banner3-left"> <h3 style="text-align:left;">STREAMLINE YOUR PATH TO DISCOVERY</h3> <h2 style="text-align:left;">Gene-to-Discovery Solutions</h2> <h3 style="text-align:left;">AAV Packaging, Custom mRNA Synthesis, Lentivirus Packaging, Recombinant Antibody Production</h3> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02gBwzs0" target="_blank"><button style="text-align:left;">LEARN MORE</button></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; } .protocol img { width: 98%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <h2>Preserve ITR INTEGRITY in aav vectors</h2> <p>Truncated ITRs can reduce the yield of full viral capsid production and increase generation of undesirable non-rAAV encapsidated DNA. Unfortunately, AAV plasmid ITRs are frequently mutated during plasmid production in bacterial cells. Azenta’s AAV plasmid preparation service incorporates a two-step QC process, including pre-prep and post-prep QC. The pre-prep QC step assesses your sample configuration for the presence of full-length intact or truncated ITRs, or a mixture of both. This allows selected propagation of intact ITR sub-populations only, while the post-prep QC step ensures integrity of ITR regions in the final plasmids delivered to you. For whole plasmid sequencing, utilize our <a href="/en/Public/Services/Sanger-Sequencing/Primer-Walking">primer walking</a> service with AAV plasmid preparation.</p> <p> </p> <a><img alt="" src="/-/media/Images/2xAAVPlasmidPreparationWorkflow2x1.ashx?w=1170px&la=en&hash=B051CB488ACE9D308D31A8686411D7B4E7220080" width="1170px"></a> </div> <p>*Plasmid DNA is currently the only starting material accepted </p> <p>Note: Screening and selection of intact ITR clones incurs additional cost</p> <br><br> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Gray margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2 style="margin: 0px;">Features & Benefits</h2> </div> <div class="headline-left"> <br /> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br></div> <div class="content-boxes-in-v3"> Robust 2-step QC process with sequence verification using Azenta’s proprietary AAV-ITR sequencing.<br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <p>Turnaround time starting at 6 business days** with Ph.D.-level support throughout project.</p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <p>High-quality midi- to giga-scale preps available.</p> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <p>High success rate compared to standard plasmid preparation kits and protocols.</p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <p>Multiple analysis options available, including sterility testing, restriction digest, full plasmid sequencing, etc. </p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Screening process to isolate intact ITRs in mixed populations. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <p class="text-left">**6-day turnaround time for samples that have previously been ITR sequence verified at Azenta.</p> <p class="text-left"> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url('/-/media/15016-D_Plasmid-EZ_WebBanFull-Med_Background.ashx'); background-size: cover; background-position: center center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 500; line-height: 100%; font-size: 50px; margin: 0; padding-bottom: 15px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h4 style="color: #826ec3;"><strong>NEW</strong></h4> <h3>Plasmid-EZ</h3> <p>Whole plasmid sequencing in as fast as 1 business day upon receipt in processing lab</p> <br /> <a href="https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ" target="_blank"> <br /> <button> LEARN MORE </button> <br /> </a> <p> </p> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-30 margin-bottom-10 padding-top-0 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h1>Technical Resources</h1> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-6'> <a href='/Public/Resources/FAQs/FAQs-Plasmid-Prep' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/chat-bubble_Purple.ashx?h=110&la=en&w=210&hash=20D4E093CF40A1D72E7CE3721F0FDEAC239B298C" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-6'> <a href='https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Plasmid-Preparation-Sample-Submission-Guidelines' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Prepare_Samples_Web-Icon-Workflow_Purple.ashx?h=91&la=en&w=91&hash=46CD2A5C3BAF8BA51A6B26A29368BBB9171BDA5C" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Sample Submission Guidelines</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; padding: 20px; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #00000; padding: 20px; margin: 0;margin-top: -20px;"> <h2></h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Tech Note" src="/-/media/Plasmid-Prep-Tech-Note-Thumbnail.ashx?h=160&w=220&la=en&hash=F37CE1AD4FC30DCAB54FD0C2849F373956556CE4" /> </div> <div class="resource resource-right"> <h3>Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins</h3> <p> </p> <p>Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.<br /> <a href="https://web.genewiz.com/technote/optimizingplasmidprep" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Tech Note: An Efficient and Reliable Approach to AAV Packaging" src="/-/media/12051-D_AAV-Packaging_TechResource-Thumb.ashx?h=160&w=220&la=en&hash=B13220BDC7AA1F511A6C578121266C42359E7B9E" /> </div> <div class="resource resource-right"> <h3>Tech Note: An Efficient and Reliable Approach to AAV Packaging</h3> <p> </p> <p>While AAV is a powerful vehicle for gene delivery, unstable ITRs threaten the fidelity of rAAV genomes. Loss of ITR integrity diminishes the packaging efficiency of rAAV particles and thus viral titer. In this tech note, discover the effect of partial and full ITR deletions on AAV packaging and how to reliably identify and effectively correct them.<br /> <a href="https://web.genewiz.com/tech-note/aav-packaging" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/AAV_Plasmid_Prep_Tech_Note_Thumbnail.ashx?la=en&hash=D2357C0C75A6F114357E189F88452B365BC39D46" /> </div> <p> </p> <div class="resource resource-right"> <h3>Tech Note: An Efficient and High-Fidelity Approach to AAV Plasmid Preparation</h3> <p> </p> <p>Azenta has recently developed a proprietary AAV plasmid preparation process, which significantly improves the chance of maintaining ITR integrity, even when other commercially available competent cells growing in low temperature have failed. This process can also isolate clones with full-length ITR from a sample mixture containing intact and truncated ITRs. <br /> <a href="https://web.genewiz.com/tech-note/aav-plasmid-prep" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/AAV_ITR_Sequencing_Thumbnail.ashx?la=en&hash=872B2FB777224F78E898037AA40F73F8EF60B9F8" /> </div> <div class="resource resource-right"> <h3>AAV-ITR Sequencing Tech Note</h3> <p> </p> <p>Azenta has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids. <br /> <a href="https://web.genewiz.com/aav-itr-tech-note" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/AAVPrepBlogUSEUWebTechThumb.ashx?la=en&hash=A7B3B9C68D8B1E659D4E5C851ADEFEC508C45936" /> </div> <div class="resource resource-right"> <h3>Article: Optimizing AAV Plasmid Preparation and ITR Sequencing</h3> <p> </p> <p>The adeno-associated virus (AAV) is a powerful vehicle for gene therapy; however, working with AAV vectors can be challenging. Spontaneous mutations in the inverted terminal repeat (ITR) regions can accumulate during plasmid propagation in bacteria. Find out how our scientists have optimized the AAV plasmid preparation process to deliver 100% accurate constructs. <br /> <a href="https://blog.genewiz.com/optimizing-aav-plasmid-preparation-and-itr-sequencing" target="_blank"> <button>Read Article</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/11019-D_AAV-Workshop_TechResource-Thumbnail.ashx?la=en&hash=0096FCC7A03D068AB61EA6E04F19AB368858AF54" /> </div> <div class="resource resource-right"> <h3>Workshop & Roundtable Discussion: A Comprehensive Guide to Using AAV Vectors in Gene Therapy</h3> <p> </p> <p>Working with AAV vectors can be challenging. In this on-demand workshop & roundtable discussion, you’ll gain a better understanding of the AAV development pipeline for gene therapy research and learn how to optimize upstream and downstream processes including AAV synthesis, sequencing, bioinformatics, and storage. <br /> <a href="https://web.genewiz.com/webinar/guide-using-aav-vectors-gene-therapy" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-3'> <a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/GWZ_GA_AAVITR_Seq_Web_Icon_Purple.ashx?h=111&la=en&w=211&hash=8D84770C7DD4D971A30969DFD5A0CEB840D66746" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>AAV-ITR Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='/en/Public/Services/Sanger-Sequencing/Premium-Services' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/premium-services-110x210_Purple.ashx?h=110&la=en&w=210&hash=EE17256D3CB28B1FC2B31A465EABD63B3EE9375D" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Primer Walking</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/plasmid-prep-overview_110x210_Purple.ashx?h=110&la=en&w=210&hash=D542B9BF1106F547CB84A8011F635AD43B95653A" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Standard Plasmid DNA Preparation</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-3'> <a href='https://www.genewiz.com/en/Public/Services/Gene-Synthesis/AAV-Packaging' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/12051-D_AAV-Packaging_Icon_AAV-Packaging.ashx?h=110&la=en&w=210&hash=2324BA98BF62F7E44E93BCB230174E16CFE40E29" alt="Building" width="210" height="110" title="AAV ITR Packaging Icon" /> </div> <div class="row"> <h3>AAV Packaging</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> <div style="text-align: center;"> <div style="text-align: center;"></div> <span style="font-size: 13px;"> </span>*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. 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GENEWIZ from Azenta | AAV Plasmid Preparation