High Throughput Gene Expression Screening

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RNA-Seq-derived approach we developed for optimal drug discovery and analysis, similar to DRUG-Seq. Unlike microarrays, our method offers rapid, unbiased, 3' gene expression screening, providing complete cell-to-data analysis of the transcriptome at a lower cost than standard RNA sequencing. Working directly from lysate, GENEWIZ High-Throughput Gene Expression Screening works directly from cell lysate, saving both time and money by removing the need for RNA purifications. 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display: inline-block; text-align: center; } .protocol img { width: auto; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <h2>HIGH-THROUGHPUT GENE EXPRESSION SCREENING WORKFLOW</h2> <p> </p> <img alt="" src="/-/media/Images/High-Throughput_Gene_Expression_workflow.ashx?w=1170px&la=en&hash=12FCE42619D3988A13C227C611292B820024E4FD" width="1170px" /></div> <br /> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <h2>High-Throughput Gene Expression Screening Assay Comparison</h2> <div class="row"> <div class="col-md-7 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td style="background-color: #706f6f;">Metric</td> <td style="background-color: #826ec3;">HT-GEx Screening</td> <td style="background-color: #706f6f;">mRNA-Seq</td> <td style="background-color: #706f6f;">Total RNA-Seq</td> <td style="background-color: #706f6f;">Iso-Seq</td> </tr> <tr class="panel-heading"> <td style="background-color: #ffffff; color:#000000;"> <strong>Reads/Sample</strong></td> <td style="background-color: #d9d3ed; color:#000000;"><strong> ~1-2M Reads</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong> <span style="text-align: center;">~10-30M Reads</span></strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong><span style="text-align: center;">~20-45M Reads</span> </strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong><span style="text-align: center;">~1-2M Reads</span> </strong></td> </tr> <tr class="panel-heading"> <td style="background-color: #ffffff; color:#000000;"><strong> Min. Sample Size</strong></td> <td style="background-color: #d9d3ed; color:#000000;"> <strong>96/384</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong> 1</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong> 1</strong></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"><strong>1 </strong></td> </tr> <tr class="panel-heading"> <td style="background-color: #ffffff; color:#000000;">Cost</td> <td style="background-color: #d9d3ed; color:#000000;"> $</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">$$ </td> <td style="text-align: center; background-color: #ffffff; color:#000000;">$$$ </td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> $$$$</td> </tr> <tr class="panel-heading"> <td style="background-color: #ffffff; color:#000000;"> Starting Material</td> <td style="background-color: #d9d3ed; color:#000000;"> Cell Lysate</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">>20ng Total RNA </td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> >100ng Total RNA</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">>1ug Total RNA </td> </tr> <tr class="panel-heading"> <td style="background-color: #ffffff; color:#000000;"> Selection Method</td> <td style="background-color: #d9d3ed; color:#000000;"> Poly(A) +</td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> <span style="text-align: center;"> Poly(A) +</span></td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> rRNA -</td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> <span style="text-align: center;"> Poly(A) +</span></td> </tr> <tr class="panel-heading"> <td style="background-color: #ffffff; color:#000000;"> Detection Level</td> <td style="background-color: #d9d3ed; color:#000000;"> 3' Tails of Genes</td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> Poly(A) Transcript</td> <td style="text-align: center; background-color: #ffffff; color:#000000;">All Transcript </td> <td style="text-align: center; background-color: #ffffff; color:#000000;"> Full-Length Poly(A) Transcript</td> </tr> <tr> <td>Gene Expression</td> <td style="background-color: #d9d3ed;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td class="text-center" style="text-align: center;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> </tr> <tr> <td style="background-color: #ffffff;"> <p>Variant Detection</p> </td> <td class="text-center" style="background-color: #d9d3ed;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td class="text-align: center" style="text-align: center;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> </tr> <tr> <td style="background-color: #ffffff;">Alternative Splicing</td> <td class="text-center" style="background-color: #d9d3ed;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 20px; float: none; vertical-align: middle; color: black;"></em></td> </tr> <tr> <td style="background-color: #ffffff;"> Transcriptome Assembly</td> <td class="text-center" style="background-color: #d9d3ed;"><em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em></td> <td style="text-align: center;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 20px; float: none; vertical-align: middle; color: black;"></em></td> </tr> </tbody> </table> </div> </div> <p><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle; color: green;"></em> Suitable method | <em class="BlueCheck icon-4x fa fa-plus" style="font-size: 20px; float: none; vertical-align: middle; color: black;"></em> Preferred method | <em class="BlueCheck icon-4x fa fa-minus" style="font-size: 20px; float: none; vertical-align: middle; color: purple;"></em> Not a suitable method</p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .rna-seq-services { width: 1170px; margin: 0 auto; text-align: center; margin-bottom: 50px; } .service-container { width: 100%; text-align: center; display: flex; justify-content: space-around; } .rna-seq-service1 { width: 30%; text-align: center; padding: 20px 0 75px 0px; 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Please <a href="https://hubs.ly/Q015tTC00">contact us</a> about how we can customize the analysis to answer your biological question.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .workflow-container { padding-top: 30px; background: #fffffff; } .workflow { text-align: center; width: 1170px; display: inline-block } .workflow-step { vertical-align: top; width: 30%; padding: 10px; margin-bottom: 20px; display: inline-block; } .workflow-step img { width: 150px; margin: 0 auto; margin-bottom: 20px; } .workflow-step h2 { width: 80%; text-align: center; } .workflow-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .workflow-step p { width: 80%; margin: 0 auto; text-align: center; } .workflow-icon { text-align: center; } @media only screen and (max-width: 1170px) { .workflow-container { width: auto; } .workflow { text-align: center; width: auto; } .workflow-step { width: 100%; } } </style> <div class="workflow-container"> <div class="workflow"> <h2 style="text-align: center; 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padding: 0; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13057-D_Transcriptomics-Webinar_TechResource_Laptop.ashx?la=en&hash=C9D7CAA35F4E698EB28DFEE74F8ADB2FA09B5276" /> </div> <div class="resource resource-right"> <h3>Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond</h3> <p>With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings. <br /> <a href="https://web.azenta.com/webinar-advancing-transcriptomics?utm_campaign=2022-05 GEN NGS - Advancing Transcriptomics Webinar&utm_source=us ht-gex&utm_medium=tech resources" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="High-Plex Spatial Analysis of Tissue Microenvironments" src="/-/media/Images/13008-D_HT-GEx_Web-Tech-Thumb.ashx?la=en&hash=665950DF0138FC7B750C09882A2B8B85727255CC" /> </div> <div class="resource resource-right"> <h3>Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening </h3> <p>High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery. <br /> <a href="https://web.genewiz.com/tech-note/high-throughput-gene-expression-screening-phenotypic-profiling" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="High-Plex Spatial Analysis of Tissue Microenvironments" src="/-/media/Images/High-throughput_sell-sheet_Tech-Thumb.ashx?la=en&hash=E68459BDC5813A103629460CFC6E06D98DF0CDF3" /> </div> <div class="resource resource-right"> <h3>Information Sheet | High-Throughput Gene Expression (HT-GEx) Screening</h3> <p>Download our information sheet for a brief overview of Azenta Life Scences' high-throughput gene expression screening service.                                         <br /> <a href="https://web.azenta.com/hubfs/ARCHIVE/Sell Sheet Collateral Library/NGS/Sell Sheets + Brochures/13008-SD 0322 HT-Gex Sell Sheet.pdf?__hstc=251210969.ee677d8374177d47808d4f65f2e7a318.1652957843001.1654783141966.1654849434556.36&__hssc=251210969.16.1654849434556&__hsfp=1723731843" target="_blank"> <button>Download</button> </a> </p> <p> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Illumina"><img alt="Illumina" 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