Next Generation Sequencing (NGS) | GENEWIZ from Azenta

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href="https://fs.hubspotusercontent00.net/hubfs/3478602/13009-SD 1221V3 Next-Gen Seq Data Quality Sell Sheet.pdf ">Our Results</a>   <a class="btn-u btn-u-lg rounded btn-u-blue" target="" href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines">Sample Submission Guidelines<br /></a>    </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; padding: 0; } .ngs-new-container { background: #f0f0f0; padding: 60px 0; margin-top: -20px; } .ngs-new-container h1 { margin: 0; color: #000000; } .ngs-new-content p { color: #000000; } .ngs-new-content { width: 1170px; margin: 0 auto; } .ngs-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #ngs-content-box-left { width: 60%; text-align: left; } #ngs-content-box-right { width: 39%; text-align: right; } .ngs-content-box img { width: 400px; } .ngs-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } .ngs-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .ngs-new-container { text-align: center; margin-top: 0; } .ngs-new-content { width: 90%; margin: 0 auto; } .ngs-new-content { display: inline-block; } #ngs-content-box-right, #ngs-content-box-left { width: 100%; text-align: center; } #ngs-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .ngs-new-content { width: 90%; margin: 0 auto; } .ngs-content-box img { width: 350px; } } </style> <div class="ngs-new-container"> <div class="ngs-new-content"> <div class="ngs-content-box" id="ngs-content-box-left"> <h1>Next Generation Sequencing (NGS)</h1> <div> </div> <p>Next generation sequencing (NGS) is a revolutionary technology that simultaneously adds nucleotides while copying a DNA strand. This process generates a signal corresponding to the nucleotide type and position of the growing DNA strand, enabling efficient DNA sequencing.</p> <p>Our next generation sequencing services include standard and custom options for extraction, library preparation, sequencing, and bioinformatics. All our NGS services are performed at our state-of-the-art laboratory in New Jersey using the latest NGS technologies, including the new Illumina<sup>®</sup> NovaSeq<sup>®</sup> X series and PacBio<sup>®</sup> Revio<sup>™</sup> platforms, to ensure you receive the highest quality data. Our Ph.D.-level scientists provide free consultations, real-time updates, and post-delivery assistance — supporting you throughout each step of your project.</p> <p>As fellow scientists and genomics leaders, we continuously optimize our processes to deliver the best results that meet your budget and deadline. Whether it’s your first NGS project or your hundredth, we are committed to helping you find the next generation sequencing service that aligns with your research needs.</p> <a name="solution"></a> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/hubfs/2023-01 GEN NGS Webpages/13006-SO 0124 NGS Overview Sell Sheet.pdf" target="_blank"><button>Download One-Sheet Overview</button>   </a><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry" target="_blank"><button>Submit Inquiry</button></a></div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02W078p0" target="_blank"><img alt="" src="/-/media/13069-D_NGS-New-Price_WebBanRight-US_20241029.ashx?la=en&hash=A766CDCF47A58336B2C9B2FC8905A93E5D1367E1" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <h2>Find the right NGS solution with our interactive tool</h2> <iframe src="https://services.cognitoforms.com/f/OQ3A98HloUi2D8ZR_2sj-A?id=9" style="position: relative; width: 1px; min-width: 100%; overflow: hidden;" frameborder="0" scrolling="no" seamless="seamless" height="810px" width="100%" data-cog-init="true"></iframe> <script src="https://services.cognitoforms.com/scripts/embed.js"></script> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default row-centered margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Next Generation Sequencing Services</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/RNA-Seq' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/GWZ_NGS_RNAseq_Web_Icon_Purple.ashx?h=111&la=en&w=211&hash=84A797481570CEA4593978E5CA7294740B782025" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>RNA-Seq</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Single-Cell-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/Single-Cell_RNA-Seq_Web-Icon-Service_Purple.ashx?h=111&la=en&w=211&hash=AA2E14FE01F52A19C59D65BF620F2DB096E2B62E" alt="Building" width="211" height="111" /> </div> <div class="row"> 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src="/-/media/Images/Home/icons/Purple-Icons/whole-genome-sequencing_110x210_Purple.ashx?h=110&la=en&w=210&hash=6FBD1B8C2E3AA5A93B5D4ABD4142E4BB8A1D9415" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Whole Genome Sequencing</h3> <br> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/exome-sequencing_110x210_Purple.ashx?h=110&la=en&w=210&hash=5739882864EC24919A7AE3FA7A7DADAC994A3029" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Exome Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' target=''> <div 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#banner-right1 button { font-size: 14px; } } </style> <div class="body1"> <div class="banner-background1"> <div id="banner-left1"> <img alt="" src="/-/media/13070-D_NGS-Platforms_WebBanFull-Lrg_Thumbnail.ashx?la=en&hash=2267956154B8A11D571A104DAF0356024C1845F7" /> </div> <div id="banner-right1"> <div id="banner-right1 h2"> <h3></h3> <h2>Take advantage of the latest long-read and short-read sequencing technology to accelerate your research</h2> <div id="banner-right1 h3"> <h3 style="color:#FFFFFF;"></h3> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms" target="_blank"><button>LEARN MORE</button></a> </div> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Gray margin-top-40 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Next Generation Sequencing Features & Benefits</h2> </div> <div class="headline-left"> <br /> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <strong>Complete sample-to-analysis solutions</strong> including customizable extraction and data analysis options available <br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br></div> <div class="content-boxes-in-v3"> <strong>Superior data quality</strong> that exceeds Illumina’s benchmarks for the NovaSeq, HiSeq™, and MiSeq™ (<a href="https://f.hubspotusercontent00.net/hubfs/3478602/NGS_ValueProp_US_Oct22_2020_NoCrops.pdf" target="_blank">Learn More</a>) </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <strong>In-house</strong> pipeline for optional Sanger-based <a href="/en/Public/Services/Molecular-Genetics/cDNA-Verification">confirmatory sequencing </a>of final targets utilizing your NGS samples </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <strong>Dedicated support</strong> from Ph.D.-level project managers plus real-time project updates so you’re never in the dark <br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <strong>Flexible service packages</strong> to meet your budget and deadline <br /> </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is Next Generation Sequencing (NGS)?</h2> <p>Next Generation Sequencing (NGS) is a technology in which nucleotides are added in parallel to the copying of a DNA strand. When nucleotides are incorporated into the growing DNA strand, a signal is generated that corresponds to the nucleotide type and position. Regardless of the distinct method used to capture nucleotide information, NGS provides a wide snapshot of the genome of interest with higher sensitivity and higher capacity with sample multiplexing.</p> <p>There are several distinct methods used to generate and capture signals that translate into a DNA sequence.</p> <h3>Nanopore/ONT (Oxford Nanopore Technologies) Sequencing:</h3> <ul> <li>Signal: Change in electrical current when a nucleotide is passed through a nanopore</li> <li>Method: The DNA sequence is determined by the change in electrical current</li> </ul> <h3>Illumina Sequencing:</h3> <ul> <li>Signal: Nucleotides are labeled with fluorescence and added to the growing DNA strand</li> <li>Method: A camera captures an image of the fluorescence signal associated with the identity of each nucleotide; the order of each fluorescence signal translates to the sequence of DNA</li> </ul> <h3>Ion Torrent Sequencing:</h3> <ul> <li>Signal: Hydrogen ions are released when a nucleotide is added, which leads to a change in pH</li> <li>Method: The sequence of DNA is determined by the changes in pH</li> </ul> <h3>PacBio (Pacific Biosciences) Sequencing:</h3> <ul> <li>Signal: Also referred to as Single-Molecule Real-Time (SMRT®) sequencing, fluorescently labeled nucleotides are observed in real-time as DNA is polymerized</li> <li>Method: The sequence of DNA is determined by the timing and order of the incorporated fluorescently labeled nucleotides</li> </ul> <p> </p> <h2>What is the importance of next generation sequencing?</h2> <p>NGS is important for any molecular biologist because of its speed. With the ability to sequence millions of DNA strands simultaneously in a single run, the fast turnaround time allows for high sample volumes and the ability to sequence up to thousands of genes or gene regions. NGS technology enables the scientist to expand their DNA snapshot – gaining a wider perspective and understanding with comprehensive genomic coverage of their sample.</p> <p> </p> <h2>What is the difference between NGS and Sanger sequencing?</h2> <p>NGS sequences millions to billions of DNA fragments in parallel, allowing for significantly high-throughput and rapid, cost-effective generation of genomic data. Sanger sequencing traditionally produces longer read lengths than NGS, at lower throughput since DNA fragments are read one at a time. Sanger sequencing is advantageous for certain applications, such as validating DNA clones, sequencing long stretches of DNA, and resolving complex genomic regions. </p> <p> </p> </div> </div> <div class="process-v1 Image-Slider Component-BackGround-Default ngs-workflow margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center margin-bottom-20"> <div class="row"> <span sc-part-of="field" class="scWebEditInput" id="fld_B0ED0DE079F84728BB1ED89C99B41E5E_3BC76BA4EE5C433DB9FDA754AA84AC64_en_1_9843fd5279dc437eaa0e5b7de2548438_1286_edit" contenteditable="false"><h2>Next Generation Sequencing Workflow</h2></span> </div> <div class="row"> Extensive Quality Control is Performed at the Conclusion of Each Step of the Process<br> </div> </div> <ul class="list-inline list-st row process-in headline-center"> <li style="text-align:center;cursor:pointer;" class="ImgSlili_1"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/custom-data-analysis-deliverables_110x210-2.ashx?h=110&la=en&w=210&hash=61D5504AE9203DBD544CA5C4333B8C118D50D573" alt="Building" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Experimental Design</h3> <br /> </div> <div class="SeqIcon SeqIcon_1"> <span class="SeqIcon_text">1</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_1"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_2"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/dample-prep-workflow_110x210.ashx?h=110&la=en&w=210&hash=73B4A79DE05EAE74D2E77FCB28728E57CABFCD02" alt="Building" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Sample Preparation</h3> <br /> </div> <div class="SeqIcon SeqIcon_2"> <span class="SeqIcon_text">2</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_2"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_3"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/library-prep-workflow_110x210.ashx?h=110&la=en&w=210&hash=72F52244BFEA9979481F926CC83C560EBF45FE87" alt="Building" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Library Preparation</h3> <br /> </div> <div class="SeqIcon SeqIcon_3"> <span class="SeqIcon_text">3</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_3"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li 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iconCount = 0; var icons; var iconContent; var Flag; jQuery(document).ready(function ($) { icons = $(".SeqIcon"); iconContent = $(".icon-content"); //hide the content if (iconContent != null && jQuery(".content_1").length > 0) { iconContent.each(function (i) { if (i > 0) { $(this).hide(); } }); } //change the icon status if (icons != null) { iconCount = icons.length; $(icons[0]).addClass("SeqIcon_Sel"); Flag = setInterval(function () { if (i == iconCount) { i = 0; } SwcRCA(i); i++; }, 2000); } $("li[class^=ImgSlili]").bind("mousemove", function () { clearInterval(Flag); jQuery("div[class^='row icon-content content_']").hide(); jQuery(".Img-arror").find("img").attr("src", "/assets/img/gwz/arrow.png"); jQuery(icons).removeClass("SeqIcon_Sel"); jQuery(".content_" + $(this).attr("class").split("_")[1]).show(); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).addClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); }); $("li[class^=ImgSlili]").bind("mouseleave", function () { i = 0; Flag = setInterval(function () { SwcRCA(i); i++; }, 2000); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).removeClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow.png"); }); }); function SwcRCA(i) { if (i == 0) { jQuery(icons[icons.length - 1]).removeClass("SeqIcon_Sel"); } else { jQuery(icons[i - 1]).removeClass("SeqIcon_Sel"); } jQuery("div[class^='row icon-content content_']").hide(); jQuery(icons[i]).addClass("SeqIcon_Sel"); jQuery(".content_" + (i + 1)).show(); //change the arrow color jQuery(".Img_" + (i)).find("img").attr("src", "/assets/img/gwz/arrow.png"); if (i != (icons.length - 1)) { jQuery(".Img_" + (i + 1)).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); } } </script> </div><script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { 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