CTGF/CCN2 Antibody [Unconjugated] - C-terminus (AF660): Novus Biologicals

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View Goat Polyclonal anti-CTGF/CCN2 Antibody [Unconjugated] - C-terminus (AF660). Validated Applications: IHC. Validated Species: Human. Sample size available." /> <meta property="og:description" content="Cited in 7 publications. View Goat Polyclonal anti-CTGF/CCN2 Antibody [Unconjugated] - C-terminus (AF660). Validated Applications: IHC. Validated Species: Human. Sample size available." /> <meta property="og:image" content="//images.novusbio.com/thumbnails2/CTGF_AF660_Immunohistochemistry_9968.jpg" /> <meta refresh_date="2/13/2025 2:00:05 PM" /> <meta name="baidu-site-verification" content="xQr2vSIwwB" /> <script id="json-ld" type="application/ld+json">[{"@context":"http://schema.org","@type":"Question","name":"We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. 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grid_16">  <h1>CTGF/CCN2 Antibody [Unconjugated] - C-terminus</h1> </div> <div class="clear"></div> <div> <div class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div class="grid_16"><div class="ds_info float_left"><a href="#PublicationSection"><div class="sprite_pubBook_large"></div></a> <a href="#PublicationSection" class="bottom2"><span class="ctr">(7 Publications)</span></a></div><div class="ds_info float_left"> <a href="/common-name/ctgf-ccn2"><div class="sprite_allRelated"></div></a> <a href="/common-name/ctgf-ccn2" class="bottom4"><span class="ctr">See All Related</span></a> </div><div class="ds_info float_left"><a href="/reviews/AF660"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/AF660" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="5" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/CTGF_AF660_Immunohistochemistry_9968.jpg" class="top_images" rel="gallery" title="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > <img src="//images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg" class="big_thumb" alt="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." /> </a> </div> <div class="alt-caption padding_left5" >CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/CTGF_AF660_Immunohistochemistry_9969.jpg" class="top_images" rel="gallery" title="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > <img src="//images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." /> </a> </div> <div class="alt-caption padding_left5" >CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg" class="top_images" rel="gallery" title="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-82202410060.jpg" class="top_images" rel="gallery" title="Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-82202410060.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to<a class="openGallery" href="#" data-picnum="3"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-44202415425071.jpg" class="top_images" rel="gallery" title="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems." > <img src="//images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-44202415425071.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems." title="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems." /> </a> </div> <div class="alt-caption padding_left5" >Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with<a class="openGallery" href="#" data-picnum="4"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Immunohistochemistry'>IHC</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Goat</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="Human CTGF/CCN2 C-Terminus Affinity Purified Polyclonal Ab" data-catnum="AF660" data-catnums="AF660,AF660-SP" data-disabled=''><noscript><form autocomplete="off" action="/products/ctgf-ccn2-antibody_af660" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # AF660-SP">25 ug</div><div data-msg='' class="atc_catnum">AF660-SP</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">8-10 Business Days</td> </tr> <tr class="odd"><td><div class="atc_size" title="catalog # AF660">100 ug</div><div data-msg='' class="atc_catnum">AF660</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">4-6 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-ie_JmJej2m4r9yYfAamdtRpjGWbFcDKQwDq4UV7ROcs" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"><div class="list_expand options_closed" data-name="Conjugates" data-wrapper-type=".conjugates_wrapper"><div class="options_text">View Available Conjugates</div> <div class="expander option_closed"></div></div><div class="conjugates_wrapper" data-title="CTGF/CCN2 Antibody [Unconjugated] - C-terminus" data-catnum="AF660"><table class="scf_table"> <thead> <tr> <th>Catalog# & Conjugate</th> <th>Size</th> <th class="col_price">Price</th> <th class="col_cart hidden"></th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="BAF660"> <td> <a href="/products/ctgf-ccn2-antibody_baf660" class="conj_link">Biotin</a><br /> <a href="/products/ctgf-ccn2-antibody_baf660" class="cat_link">BAF660</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart hidden"><a href="/commerce/add-to-cart/414783"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr> <tr class="scf_footer"> <td colspan="4"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["BAF660"]' data-rendered="false"></div></div><div class="list_expand options_closed" data-name="Formulations" data-wrapper-type=".formulations_wrapper"><div class="options_text">View Available Formulations</div> <div class="expander option_closed"></div></div><div class="formulations_wrapper" data-title="CTGF/CCN2 Antibody [Unconjugated] - 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C-terminus Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div><EM>E. coli</EM>-derived recombinant human CTGF/CCN2 C-Terminus<br />Glu247-Ala349<br />Accession # P29279</div></td> </tr><tr class="lined"> <td><strong>Specificity</strong></td> <td><div>Detects human CTGF/CCN2 C-Terminus in direct ELISAs. In direct ELISAs, approximately 15% cross‑reactivity with recombinant rat CTGF is observed.</div></td> </tr><tr class="lined"> <td><strong>Source</strong></td> <td><div>N/A</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Goat</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>CTGF</div></td> </tr><tr class="lined"> <td><strong>Purity Statement</strong></td> <td><div>Antigen Affinity-purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Immunohistochemistry 5-15 ug/mL</li> </ul> </td> </tr><tr class="lined"> <td><strong>Application Notes</strong></td> <td><div>Use in FLOW application reported in PMID: 22493011</div></td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> AF660 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC-P">IHC-P</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Western Blot">Western Blot</a> </div><div class="information-data">1 publication</div></li></ul></td> </tr> </table> </td> </tr> </table> </div><h2 class="greyHead2">Reactivity Notes</h2> <div> <a id="tab1speciesreactivity"></a> <div class="ds_info"> Reactivity with Mouse is reported in PMIDs: 22493011 and 21602472</div> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>No Preservative</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr><tr class="lined"> <td><strong>Reconstitution Instructions</strong></td> <td><div>Reconstitute at 0.2 mg/mL in sterile PBS.</div></td> </tr></table></div> <h2 class="greyHead2">Notes</h2><div class="ds_info"> <a id="tab1notes"></a> <div>This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.</div> </div><h2 class="greyHead2">Alternate Names for CTGF/CCN2 Antibody [Unconjugated] - C-terminus</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>CCN2</li><li>CCN2IGFBP-8</li><li>connective tissue growth factor</li><li>CTGF</li><li>CTGRP</li><li>Fisp12</li><li>HCS24</li><li>Hypertrophic chondrocyte-specific protein 24</li><li>IBP-8</li><li>IGF-binding protein 8</li><li>IGFBP-8</li><li>IGFBP8CCN family member 2</li><li>Insulin-like growth factor-binding protein 8</li><li>MGC102839</li><li>NOV2</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div>CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contributes to the adhesive and mitogenic properties of CTGF.</div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1640"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/nov-ccn3-antibody_af1640" style="overflow: hidden; width: 280px;" title="NOV/CCN3 Antibody [Unconjugated]">NOV/CCN3 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF1640</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/nov-ccn3-antibody_af1640" title="NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 &amp;micro;g/mL overnight at 4 &amp;deg;C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images2/NOV_AF1640_Immunohistochemistry_7314.jpg" alt="Immunohistochemistry NOV/CCN3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/nov-ccn3-antibody_af1640#PublicationSection"> <div class="sprite_pubBook"></div> (4 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-91258"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/fibronectin-antibody_nbp1-91258" style="overflow: hidden; width: 280px;" title="Fibronectin Antibody - BSA Free">Fibronectin<br> Antibody - BSA Free</a>    </div><div class="sub-header">NBP1-91258</div> <div class="images_customers"><div id="ss_prev_cav1" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-1" data-cycle-prev="#ss_prev_cav1" data-cycle-next="#ss_next_cav1" data-cycle-slides="div.big_thumb_cust_container" data-index=1 ><div class="big_thumb_cust_container"> <a href="/products/fibronectin-antibody_nbp1-91258" title="Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were intraperitoneally injected an hour before the surgery and daily thereafter. Representative images of Western blot of FN (fibronectin), alpha-SMA (alpha-smooth muscle actin) and GAPDH (loading control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30993884/) licensed under a CC-BY license." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images/Fibronectin Antibody - BSA Free-Western Blot-NBP1-91258-img0022.jpg" alt="Western Blot Fibronectin Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/fibronectin-antibody_nbp1-91258" title="Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0003.jpg" alt="Western Blot Fibronectin Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/fibronectin-antibody_nbp1-91258" title="Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0004.jpg" alt="Western Blot Fibronectin Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/fibronectin-antibody_nbp1-91258#ReviewsSection"> <div class="sprite_4Stars"></div> (11 Reviews</a>) </div> <div class="tiny"> <a href="/products/fibronectin-antibody_nbp1-91258#PublicationSection"> <div class="sprite_pubBook"></div> (56 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="7754-BH/CF"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tgf-beta-1-human-cell-expressed-cf_7754-bh-cf" style="overflow: hidden; width: 280px;" title="TGF-beta 1 [Unconjugated]">TGF-beta 1<br> [Unconjugated]</a>    </div><div class="sub-header">7754-BH/CF</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev_cav2" data-cycle-next="#ss_next_cav2" data-cycle-slides="div.big_thumb_cust_container" data-index=2 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tgf-beta-1-human-cell-expressed-cf_7754-bh-cf" title="Recombinant human TGF-beta 1 (7754-BH/CF) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg" alt="Bioactivity TGF-beta 1 [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tgf-beta-1-human-cell-expressed-cf_7754-bh-cf#PublicationSection"> <div class="sprite_pubBook"></div> (53 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF4055"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/cyr61-ccn1-antibody_af4055" style="overflow: hidden; width: 280px;" title="Cyr61/CCN1 Antibody [Unconjugated]">Cyr61/CCN1<br> Antibody [Unconjugate</a>    </div><div class="sub-header">AF4055</div> <div class="images_customers"><div id="ss_prev_cav3" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/cyr61-ccn1-antibody_af4055" title="Western blot shows lysates of mouse spleenocyte cell, RAW 264.7 mouse monocyte/macrophage cell line, NIH-3T3 mouse embryonic fibroblast cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/Cyr61_AF4055_Western_Blot_5394.jpg" alt="Western Blot Cyr61/CCN1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cyr61-ccn1-antibody_af4055" title="Cyr61/CCN1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 &amp;micro;g/mL Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) overnight at 4 &amp;deg;C. Tissue was stained with the Anti-Sheep HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/Cyr61_AF4055_Immunohistochemistry_6819.jpg" alt="Immunohistochemistry Cyr61/CCN1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/cyr61-ccn1-antibody_af4055" title=" Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 51&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of Sheep Anti-Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog&amp;nbsp;# HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images2/Cyr61_AF4055_Simple_Western_20498.jpg" alt="Simple Western Cyr61/CCN1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/cyr61-ccn1-antibody_af4055#PublicationSection"> <div class="sprite_pubBook"></div> (13 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DVE00"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" style="overflow: hidden; width: 280px;" title="VEGF [HRP]">VEGF [HRP]</a>    </div><div class="sub-header">DVE00</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00" title="" class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg" alt="N/A VEGF [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-vegf-quantikine-elisa-kit_dve00#PublicationSection"> <div class="sprite_pubBook"></div> (685 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk2-antibody_af1230" style="overflow: hidden; width: 280px;" title="ERK2 Antibody [Unconjugated]">ERK2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF1230</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg" alt="Western Blot ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" alt="Immunohistochemistry ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" alt="Knockout Validated ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk2-antibody_af1230#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-22203"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk1-antibody-1e5_nbp2-22203" style="overflow: hidden; width: 280px;" title="ERK1 Antibody (1E5) - BSA Free">ERK1 Antibody<br> (1E5) - BSA Free</a>    </div><div class="sub-header">NBP2-22203</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red)." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg" alt="Flow Cytometry ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg" alt="Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk1-antibody-1e5_nbp2-22203#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Pm, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF4309"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/plexin-a1-antibody_af4309" style="overflow: hidden; width: 280px;" title="Plexin A1 Antibody [Unconjugated]">Plexin A1 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF4309</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/plexin-a1-antibody_af4309" title="Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunohistochemistry_10417.jpg" alt="Immunohistochemistry Plexin A1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/plexin-a1-antibody_af4309" title="Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunocytochemistry_11095.jpg" alt="Immunocytochemistry Plexin A1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/plexin-a1-antibody_af4309" title="Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/Plexin_A1_AF4309_Western_Blot_9601.jpg" alt="Western Blot Plexin A1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/plexin-a1-antibody_af4309#PublicationSection"> <div class="sprite_pubBook"></div> (12 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ICC, IHC, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-84037"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/rpl10-antibody_nbp1-84037" style="overflow: hidden; width: 280px;" title="RPL10 Antibody">RPL10 Antibody</a>    </div><div class="sub-header">NBP1-84037</div> <div class="images_customers"><div id="ss_prev_cav8" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/rpl10-antibody_nbp1-84037" title="Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/RPL10-Antibody-Immunohistochemistry-Paraffin-NBP1-84037-img0014.jpg" alt="Immunohistochemistry-Paraffin RPL10 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rpl10-antibody_nbp1-84037" title="Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining of human colon shows strong cytoplasmic positivity in glandular cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/RPL10-Antibody-Immunohistochemistry-Paraffin-NBP1-84037-img0015.jpg" alt="Immunohistochemistry-Paraffin RPL10 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/rpl10-antibody_nbp1-84037" title="Immunohistochemistry-Paraffin: RPL10 Antibody [NBP1-84037] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/RPL10-Antibody-Immunohistochemistry-Paraffin-NBP1-84037-img0016.jpg" alt="Immunohistochemistry-Paraffin RPL10 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/rpl10-antibody_nbp1-84037#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="210-TA"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" style="overflow: hidden; width: 280px;" title="TNF-alpha [Unconjugated]">TNF-alpha<br> [Unconjugated]</a>    </div><div class="sub-header">210-TA</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg" alt="SDS-PAGE TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg" alt="Bioactivity TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta" title="Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg" alt="SEC-MALS TNF-alpha [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#ReviewsSection"> <div class="sprite_5Stars"></div> (3 Reviews</a>) </div> <div class="tiny"> <a href="/products/recombinant-human-tnf-alpha-protein_210-ta#PublicationSection"> <div class="sprite_pubBook"></div> (821 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-30027"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/serpin-a8-angiotensinogen-antibody_nbp1-30027" style="overflow: hidden; width: 280px;" title="Serpin A8/Angiotensinogen Antibody - BSA Free">Serpin<br> A8/Angiotensinogen An ...</a>    </div><div class="sub-header">NBP1-30027</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/serpin-a8-angiotensinogen-antibody_nbp1-30027" title="Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Serpin-A8-Angiotensinogen-Antibody-Immunohistochemistry-Paraffin-NBP1-30027-img0012.jpg" alt="Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/serpin-a8-angiotensinogen-antibody_nbp1-30027" title="Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Serpin-A8-Angiotensinogen-Antibody-Western-Blot-NBP1-30027-img0013.jpg" alt="Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/serpin-a8-angiotensinogen-antibody_nbp1-30027" title="Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody - BSA Free [NBP1-30027] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of Serpin A8 (NBP1-30027) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Serpin-A8-Angiotensinogen-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-30027-img0015.jpg" alt="Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/serpin-a8-angiotensinogen-antibody_nbp1-30027#PublicationSection"> <div class="sprite_pubBook"></div> (12 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF3797"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad2-3-antibody_af3797" style="overflow: hidden; width: 280px;" title="Smad2/3 Antibody [Unconjugated]">Smad2/3 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF3797</div> <div class="images_customers"><div id="ss_prev_cav11" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/smad2-3-antibody_af3797" title="Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad3_AF3797_Western_Blot_5347.jpg" alt="Western Blot Smad2/3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad2-3-antibody_af3797" title="Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog&amp;nbsp;# AB‑108‑C) for 15&amp;nbsp;minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog&amp;nbsp;# MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad3_AF3797_Chromatin_Immunoprecipitation_11240.jpg" alt="Chromatin Immunoprecipitation (ChIP) Smad2/3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad2-3-antibody_af3797" title="Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and, in EMT-induced cells, nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad3_AF3797_Immunocytochemistry_11829.jpg" alt="Immunocytochemistry Smad2/3 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/smad2-3-antibody_af3797#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/smad2-3-antibody_af3797#PublicationSection"> <div class="sprite_pubBook"></div> (24 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ChIP, ICC, IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-56479"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad3-antibody_nb100-56479" style="overflow: hidden; width: 280px;" title="Smad3 Antibody">Smad3 Antibody</a>    </div><div class="sub-header">NB100-56479</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/smad3-antibody_nb100-56479" title="Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Smad3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56479-img0006.jpg" alt="Immunocytochemistry/ Immunofluorescence Smad3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad3-antibody_nb100-56479" title="Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Smad3-Antibody-Western-Blot-NB100-56479-img0008.jpg" alt="Western Blot Smad3 Antibody" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad3-antibody_nb100-56479" title="Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Smad3-Antibody-Immunohistochemistry-Paraffin-NB100-56479-img0004.jpg" alt="Immunohistochemistry-Paraffin Smad3 Antibody" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/smad3-antibody_nb100-56479#ReviewsSection"> <div class="sprite_3Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/smad3-antibody_nb100-56479#PublicationSection"> <div class="sprite_pubBook"></div> (8 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ca, Hu, Mu, Po, Pm, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="M6000B"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" style="overflow: hidden; width: 280px;" title="IL-6 [HRP]">IL-6 [HRP]</a>    </div><div class="sub-header">M6000B</div> <div class="images_customers"><div id="ss_prev_cav13" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="" class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="" class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b" title="Increased production of proinflammatory cytokines and VEGF in the plasma and chondrocytes in high fat diet (HFD)-feeding mice.(a~d) levels of plasma IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the C57BL/6 male mice fed with a normal diet (ND) or HFD for 8 weeks. (n = 5, **P &lt; 0.01). (e~h) Relative mRNA expression of IL-6 (e), TNF-alpha (f), IL-1 beta (g) and VEGF (h) in the chondrocytes isolated from the 8-week ND or HFD-feeding mice. (n = 5, **P &lt; 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26271607), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_13" > <img loading="lazy" src="https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg" alt="N/A IL-6 [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav13" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-13" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/mouse-il-6-quantikine-elisa-kit-2-plate_m6000b#PublicationSection"> <div class="sprite_pubBook"></div> (676 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Mu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DCP00"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-ccl2-mcp-1-quantikine-elisa-kit_dcp00" style="overflow: hidden; width: 280px;" title="CCL2/JE/MCP-1 [HRP]">CCL2/JE/MCP-1 [HRP]</a>    </div><div class="sub-header">DCP00</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/human-ccl2-mcp-1-quantikine-elisa-kit_dcp00" title="" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/CCL2_DCP00_ELISA_67.jpg" alt="N/A CCL2/JE/MCP-1 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-ccl2-mcp-1-quantikine-elisa-kit_dcp00" title="" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_633.jpg" alt="N/A CCL2/JE/MCP-1 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-ccl2-mcp-1-quantikine-elisa-kit_dcp00" title="" class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_634.jpg" alt="N/A CCL2/JE/MCP-1 [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-ccl2-mcp-1-quantikine-elisa-kit_dcp00#PublicationSection"> <div class="sprite_pubBook"></div> (258 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF467"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ephb2-antibody_af467" style="overflow: hidden; width: 280px;" title="EphB2 Antibody [Unconjugated]">EphB2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF467</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title=" Simple Western lane view shows lysates of COLO&amp;nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&amp;nbsp;kDa (as indicated) using 20&amp;nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg" alt="Simple Western EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg" alt="Western Blot EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg" alt="Immunocytochemistry EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/ephb2-antibody_af467#ReviewsSection"> <div class="sprite_4Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/ephb2-antibody_af467#PublicationSection"> <div class="sprite_pubBook"></div> (63 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, ICC, IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DTM100"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-timp-1-quantikine-elisa-kit_dtm100" style="overflow: hidden; width: 280px;" title="TIMP-1 [HRP]">TIMP-1 [HRP]</a>    </div><div class="sub-header">DTM100</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/human-timp-1-quantikine-elisa-kit_dtm100" title="" class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/DATA_TIMP1_DTM100_ELISA_847.jpg" alt="N/A TIMP-1 [HRP]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/human-timp-1-quantikine-elisa-kit_dtm100" title="" class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/TIMP-1_DTM100_ELISA_194.jpg" alt="N/A TIMP-1 [HRP]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-timp-1-quantikine-elisa-kit_dtm100#PublicationSection"> <div class="sprite_pubBook"></div> (97 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="DTSP10"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/human-thrombospondin-1-quantikine-elisa-kit_dtsp10" style="overflow: hidden; width: 280px;" title="Thrombospondin-1 [HRP]">Thrombospondin-1<br> [HRP]</a>    </div><div class="sub-header">DTSP10</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/human-thrombospondin-1-quantikine-elisa-kit_dtsp10" title="" class="cust_viewed_17" > <img loading="lazy" src="https://images.novusbio.com/images2/Thrombospondin-1_DTSP10_ELISA_199.jpg" alt="N/A Thrombospondin-1 [HRP]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/human-thrombospondin-1-quantikine-elisa-kit_dtsp10#PublicationSection"> <div class="sprite_pubBook"></div> (43 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for CTGF/CCN2 Antibody (AF660)(7)</h2><div class="ds_info"> <div>We have publications tested in 3 confirmed species: Human, Mouse, Rat.<br /><br />We have publications tested in 2 applications: IHC-P, Western Blot.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC-P"> <div style="display:inline;">IHC-P</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Western Blot"> <div style="display:inline;">Western Blot</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">7</span> of 7.<table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using AF660</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/35480397" target="_blank" rel="nofollow"> Zhaoping Qin, Tianyuan He, Chunfang Guo, Taihao Quan Age-Related Downregulation of CCN2 Is Regulated by Cell Size in a YAP/TAZ-Dependent Manner in Human Dermal Fibroblasts: Impact on Dermal Aging JID Innovations 5/1/2022 [PMID: 35480397] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31004409" target="_blank" rel="nofollow"> Jun Yoshino, Bruce W. Patterson, Samuel Klein Adipose Tissue CTGF Expression is Associated with Adiposity and Insulin Resistance in Humans Obesity (Silver Spring) 6/1/2019 [PMID: 31004409] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="IHC-P" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26176839" target="_blank" rel="nofollow"> Riordan D, Varma S, West R, Brown P Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 2015-07-15;10(7):e0128975. 2015-07-15 [PMID: 26176839] (IHC-P, Human) </a></td> <td valign="top">IHC-P</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/26022278" target="_blank" rel="nofollow"> Yang X, Qiu PC, Chen BB et al. KIAA1199 as a potential diagnostic biomarker of rheumatoid arthritis related to angiogenesis. Arthritis Research & Therapy. 1/1/2015 [PMID: 26022278] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23435419" target="_blank" rel="nofollow"> Finger EC, Cheng CF, Williams TR et al. CTGF is a therapeutic target for metastatic melanoma. Oncogene 2/25/2013 [PMID: 23435419] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21602472" target="_blank" rel="nofollow"> McCurdy S, Dai Q, Zhang J, Zamilpa R, Ramirez T, Dayah T, Nguyen N, Jin Y, Bradshaw A, Lindsey M SPARC mediates early extracellular matrix remodeling following myocardial infarction. Am J Physiol Heart Circ Physiol, 2011-05-20;301(2):H497-505. 2011-05-20 [PMID: 21602472] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="IHC-P" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/12945801" target="_blank" rel="nofollow"> Inkinen K, Wolff H, Lindroos P, Ahonen J Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue. Connect. Tissue Res., 2003-01-01;44(1):19-29. 2003-01-01 [PMID: 12945801] (IHC-P, Rat) </a></td> <td valign="top">IHC-P</td> <td valign="top">Rat</td> </tr></tbody> </table> </div> </div> <a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>CTGF/CCN2 Antibody (AF660) (0) </span></h2> <div class="ds_info"> There are no reviews for CTGF/CCN2 Antibody (AF660). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. <br/> <ul> <li>Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen</li> <li>Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen</li> </ul> <div class="align-right"> <a href="/reviews/AF660"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">FAQs for CTGF/CCN2 Antibody (AF660). (Showing 1 - <span id="faqcnt">1</span> of 1 FAQs).</h2> <a id="faqs"></a> <ol id="faq_list"><li class="faqwrap "> <span class="faq_question">We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.</span> <ul class="faq_answer"> <li>Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.</li> </ul> </li></ol> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=HRP">Anti-Goat HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=FITC">Anti-Goat FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=Biotin">Anti-Goat Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Goat%22">View all of our anti-Goat Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Goat%22">Goat Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional CTGF/CCN2 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array AF660</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/ctgf-ccn2">CTGF/CCN2 Antibodies</a></li><li> <a href="/elisa-kits/ctgf-ccn2">CTGF/CCN2 ELISA Kits</a></li><li> <a href="/lysates/ctgf-ccn2">CTGF/CCN2 Lysate</a></li><li> <a href="/proteins/ctgf-ccn2">CTGF/CCN2 Proteins</a></li><li> <a href="/proteins-and-enzymes/ctgf-ccn2">CTGF/CCN2 Proteins and Enzymes</a></li><li> <a href="/elisas/ctgf-ccn2">CTGF/CCN2 ELISA</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on CTGF/CCN2</h2> <a id="blogsect"></a> <div>There are no specific blogs for CTGF/CCN2, but you can <a href="/antibody-news/">read our latest blog posts.</div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="AB-108-C"> <div class="img_container"> <a href="/products/igg-isotype-control_ab-108-c" title="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_AB108C_Control_19376.jpg" title="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." alt="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_ab-108-c">Goat IgG Isotype Control [Unconjugated]</a> <br />AB-108-C</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_ab-108-c';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a 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Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="concentration" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button"/> </div> </div> </div> </div> </div> <h2 class="greyHead2">Review this Product</h2> <div style="padding:10px;"> Be the first to review our CTGF/CCN2 Antibody [Unconjugated] - C-terminus and receive a gift card or discount.<br /><br /> <div class="text_center"> <div class="dsSprite_stars5" style="position:relative;top:3px;"></div> <a href="/reviews/AF660"><button class="greybutton btn_width" name="novusreview">Submit a Review</button></a><br /> </div> </div><h2 class="greyHead2">Bioinformatics</h2> <div class="ds_info"> <a id="tab1bioinformatics"></a> <div> <table class="ds_list_right wide top"> <tr class="lined"> <td><strong>Gene Symbol</strong></td> <td><a href="/gene-name/CTGF">CTGF</a></td> </tr><tr class="grey-line-top"> <td><strong>Uniprot</strong></td> <td> <ul class="bioinf-list"><li><a target="_blank" href="http://www.uniprot.org/uniprot/?query=P29279" rel="nofollow"><div class="sprite_newWindow"> </div> P29279</a><span class="inline ">()</span></li> </ul> </td> </tr></table> </div> </div> </div> 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