Smad2/3 Antibody [Unconjugated] (AF3797): Novus Biologicals

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</div><div class="ds_info float_left"><a href="/reviews/AF3797"><div class="dsSprite_submitreview"></div></a> <a href="/reviews/AF3797" class="bottom4"><span class="ctr">Submit a Review</span></a></div></div></div><div class="modalWrapper" style="display:none;"> <h2>Species Glossary</h2> <hr class="customhr"/> <img src="//images.novusbio.com/design/bunny.gif" alt="Species Glossary Rabbit" style="float: right;"> <table class="modalTable"> <tr> <td>At</td> <td>=</td> <td>A. thaliana</td> <td>SyHa</td> <td>=</td> <td>Golden Syrian Hamster</td> </tr> <tr> <td>All</td> <td>=</td> <td>All Species</td> <td>Ha</td> <td>=</td> <td>Hamster</td> </tr> <tr> <td>An</td> <td>=</td> <td>Animal</td> <td>Hu</td> <td>=</td> <td>Human</td> </tr> <tr> <td>ArHa</td> <td>=</td> <td>Armenian Hamster</td> <td>I</td> <td>=</td> <td>Insect</td> </tr> <tr> <td>Av</td> <td>=</td> <td>Avian</td> <td>Ll</td> <td>=</td> <td>Llama</td> </tr> <tr> <td>Bb</td> <td>=</td> <td>Baboon</td> <td>Ma</td> <td>=</td> <td>Mammal</td> </tr> <tr> <td>Ba</td> <td>=</td> <td>Bacteria</td> <td>Mk</td> <td>=</td> <td>Monkey</td> </tr> <tr> <td>Bv</td> <td>=</td> <td>Bovine</td> <td>Mu</td> <td>=</td> <td>Mouse</td> </tr> <tr> <td>Ca</td> <td>=</td> <td>Canine</td> <td>Multi</td> <td>=</td> <td>Multi-species</td> </tr> <tr> <td>Ce</td> <td>=</td> <td>C. Elegans</td> <td>NA</td> <td>=</td> <td>Non-species specific</td> </tr> <tr> <td>Ch</td> <td>=</td> <td>Chicken</td> <td>Or</td> <td>=</td> <td>Orangutan</td> </tr> <tr> <td>ChHa</td> <td>=</td> <td>Chinese Hamster</td> <td>Pl</td> <td>=</td> <td>Plant</td> </tr> <tr> <td>Cm</td> <td>=</td> <td>Cynomolgus Monkey</td> <td>Pm</td> <td>=</td> <td>Primate</td> </tr> <tr> <td>Do</td> <td>=</td> <td>Donkey</td> <td>Po</td> <td>=</td> <td>Porcine</td> </tr> <tr> <td>Dr</td> <td>=</td> <td>Drosophilia</td> <td>Rb</td> <td>=</td> <td>Rabbit</td> </tr> <tr> <td>Ec</td> <td>=</td> <td>E. Coli</td> <td>Rt</td> <td>=</td> <td>Rat</td> </tr> <tr> <td>Eq</td> <td>=</td> <td>Equine</td> <td>RM</td> <td>=</td> <td>Rhesus Macaque</td> </tr> <tr> <td>Fe</td> <td>=</td> <td>Feline</td> <td>Sh</td> <td>=</td> <td>Sheep</td> </tr> <tr> <td>Ft</td> <td>=</td> <td>Ferret</td> <td>Vb</td> <td>=</td> <td>Vertebrate</td> </tr> <tr> <td>Fi</td> <td>=</td> <td>Fish</td> <td>Vi</td> <td>=</td> <td>Virus</td> </tr> <tr> <td>Fu</td> <td>=</td> <td>Fungi</td> <td>Xp</td> <td>=</td> <td>Xenopus</td> </tr> <tr> <td>Ge</td> <td>=</td> <td>Gerbil</td> <td>Ye</td> <td>=</td> <td>Yeast</td> </tr> <tr> <td>GP</td> <td>=</td> <td>Guinea Pig</td> <td>Ze</td> <td>=</td> <td>Zebrafish</td> </tr> <tr> <td>Gt</td> <td>=</td> <td>Goat</td> </tr> </table> <div style="text-align: center;margin-left:25px;margin-right:25px;"> <p>Note: Mouseover a species abbreviation on the product page to display the fullname.</p> </div> </div> </div><div class="clear"></div><div class="grid_10 pos_relative"><div class="ds_blue_border equaltop"><div class="imagesleft equaltop"><h2 class="blueHead">Images</h2> <div class="midBlue"> </div><div class="images"><div id="ss_prev_top" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-top" data-cycle-prev="#ss_prev_top" data-cycle-next="#ss_next_top" data-cycle-caption="#image-caption" data-cycle-caption-template="Image {{slideNum}} of {{slideCount}}" data-cycle-slides=".big_thumb_container" data-cycle-carousel-visible="5" id="images_cycle" > <div class="sub_image_wrapper"><div id="cycle-circle-pager-top" class="cycle-circle-pager"></div><div id="image-caption"></div> <a class="enlargeTop">(<div class="dsSprite_magGlass"></div> Enlarge)</a></div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/Smad3_AF3797_Western_Blot_5347.jpg" class="top_images" rel="gallery" title="Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." > <img src="//images.novusbio.com/images2/Smad3_AF3797_Western_Blot_5347.jpg" class="big_thumb" alt="Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." title="Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." /> </a> </div> <div class="alt-caption padding_left5" >Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with<a class="openGallery" href="#" data-picnum="0"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/Smad3_AF3797_Chromatin_Immunoprecipitation_11240.jpg" class="top_images" rel="gallery" title="Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR." > <img src="//images.novusbio.com/images2/Smad3_AF3797_Chromatin_Immunoprecipitation_11240.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR." title="Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR." /> </a> </div> <div class="alt-caption padding_left5" >Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin.<a class="openGallery" href="#" data-picnum="1"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/Smad3_AF3797_Immunocytochemistry_11829.jpg" class="top_images" rel="gallery" title="Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and, in EMT-induced cells, nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > <img src="//images.novusbio.com/images2/Smad3_AF3797_Immunocytochemistry_11829.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and, in EMT-induced cells, nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and, in EMT-induced cells, nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." /> </a> </div> <div class="alt-caption padding_left5" >Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3<a class="openGallery" href="#" data-picnum="2"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/Smad2_3_AF3797_Simple_Western_20385.jpg" class="top_images" rel="gallery" title=" Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad2/3 at approximately 64 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " > <img src="//images.novusbio.com/images2/Smad2_3_AF3797_Simple_Western_20385.jpg" loading="lazy" style="display:none;" class="big_thumb" alt=" Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad2/3 at approximately 64 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " title=" Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad2/3 at approximately 64 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " /> </a> </div> <div class="alt-caption padding_left5" > Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific<a class="openGallery" href="#" data-picnum="3"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/af3797_human-mouse-smad2-3-affinity-purified-polyclonal-ab-immunohistochemistry-308202115308.jpg" class="top_images" rel="gallery" title="Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > <img src="//images.novusbio.com/images2/af3797_human-mouse-smad2-3-affinity-purified-polyclonal-ab-immunohistochemistry-308202115308.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." /> </a> </div> <div class="alt-caption padding_left5" >Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 3 µg/mL for 1<a class="openGallery" href="#" data-picnum="4"> ...read more</a></div> </div> <div class="big_thumb_container"> <div class="image_wrapper"> <a href="//images.novusbio.com/fullsize2/af3797_human-mouse-smad2-3-affinity-purified-polyclonal-ab-immunohistochemistry-3082021153223.jpg" class="top_images" rel="gallery" title="Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > <img src="//images.novusbio.com/images2/af3797_human-mouse-smad2-3-affinity-purified-polyclonal-ab-immunohistochemistry-3082021153223.jpg" loading="lazy" style="display:none;" class="big_thumb" alt="Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." /> </a> </div> <div class="alt-caption padding_left5" >Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL<a class="openGallery" href="#" data-picnum="5"> ...read more</a></div> </div></div><div id="ss_next_top" class="cycle-pager"><div class="pager_right_on"></div></div></div></div><div class="productsright pos_relative equaltop"><h2 class="blueHead">Product Details</h2> <div class="midBlue">Summary</div><table class="ds_list"><tr class="lined"> <td><strong>Reactivity</strong></td> <td><span title='Human'>Hu</span>, <span title='Mouse'>Mu</span><span class="myModal">Species Glossary</span></td> </tr><tr class="lined"> <td><strong>Applications</strong></td> <td><span title='Western Blot'>WB</span>, <span title='Simple Western'>Simple Western</span>, <span title='Immunohistochemistry'>IHC</span>, <span title='Chromatin Immunoprecipitation'>ChIP</span>, <span title='Immunocytochemistry/ Immunofluorescence'>ICC/IF</span></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Goat</div></td> </tr><tr class="lined"> <td><strong>Conjugate</strong></td> <td><div>Unconjugated</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr></table></div> </div></div><div class="grid_6"><div></div> <div class="ds_blue_border equaltop"> <h2 class="blueHead_nopadding">Order Details</h2><ul class="ds_details"><li class="add-to-cart" data-type="full" data-prodname="Human/Mouse Smad2/3 Affinity Purified Polyclonal Ab" data-catnum="AF3797" data-catnums="AF3797,AF3797-SP" data-disabled=''><noscript><form autocomplete="off" action="/products/smad2-3-antibody_af3797" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # AF3797-SP">25 ug</div><div data-msg='' class="atc_catnum">AF3797-SP</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">11-13 Business Days</td> </tr> <tr class="odd"><td><div class="atc_size" title="catalog # AF3797">100 ug</div><div data-msg='' class="atc_catnum">AF3797</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">7-9 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-6t2IrxaJN7gxGtpAERy19Hjz105E6F9Bl_ayLzpi3gc" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript></li></ul><div id="shipping" class="hidden"></td></tr></div><div class="conj_form_buttons"><div class="list_expand options_closed" data-name="Formulations" data-wrapper-type=".formulations_wrapper"><div class="options_text">View Available Formulations</div> <div class="expander option_closed"></div></div><div class="formulations_wrapper" data-title="Smad2/3 Antibody [Unconjugated]" data-catnum="AF3797"><table class="scf_table"> <thead> <tr> <th>Catalog# & Formulation</th> <th>Size</th> <th class="col_price">Price</th> <th class="col_cart"></th> </tr> </thead> <tbody><tr class="popup_sku" data-sku="AF3797-SP"> <td> <a href="/products/smad2-3-antibody_af3797" class="conj_link">Carrier Free</a><br /> <a href="/products/smad2-3-antibody_af3797" class="cat_link">AF3797-SP</a> </td> <td><div class="popup_size"></div></td> <td class="col_price"><div class="popup_price"></div></td> <td class="col_cart"><a href="/commerce/add-to-cart/429744"><div class="purchase_cart"></div></a></td> </tr> <tr> <td colspan="4" class="row_stock"><div class="popup_stock"></div></td> </tr> <tr class="scf_footer"> <td colspan="4"><div class="expander option_opened"></div></td> </tr> </tbody> </table> <div class="popup_skus" data-skus='["AF3797-SP"]' data-rendered="false"></div></div></div><div id="shipping_statement"><span id="shipping_rate"></span></div><div class="learn_more"> <a href="/guarantee"> <div class="sprite_noImage" title="Novus Biologicals offers 100% Guarantee"></div> <div class="learn_more_text">Novus Biologicals offers 100% Guarantee</div></a> </div><span id="conj_form" class="hidden"><tr><td colspan="4"></td></tr></span> </div> </div><div class="clear"></div><div class="grid_10"><div><div id="ds_tabs" style="display:none;"> <div class="ds_tab_active" id="dsTabs_datasheet" data-anchor_desc="datasheet">Datasheet</div> <div class="ds_tab" id="dsTabs_reviews-publications" data-anchor_desc="reviews-publications">Reviews & Publications</div> <div class="ds_tab" id="dsTabs_protocols-faqs" data-anchor_desc="protocols-faqs">Protocols & FAQs</div> <div class="ds_tab" id="dsTabs_supportresearch" data-anchor_desc="supportresearch">Support & Research</div> </div><div class="ds_tab_content equalbottom" id="content_datasheet" > <a id="datasheet"></a><h2 class=greyHead2>Smad2/3 Antibody [Unconjugated] Summary</h2> <div class="ds_info"> <a id="tab1details"></a> <table class="ds_list wide" style="table-layout:fixed;"> <tr class="lined"> <td><strong>Immunogen</strong></td> <td><div><EM>E. coli</EM>-derived recombinant human Smad3<br />Ser2-Ala230<br />Accession # P84022</div></td> </tr><tr class="lined"> <td><strong>Specificity</strong></td> <td><div>Detects human, mouse, and rat Smad2/3 in Western blots. Predicted to detect rat based on sequence homology.</div></td> </tr><tr class="lined"> <td><strong>Source</strong></td> <td><div>N/A</div></td> </tr><tr class="lined"> <td><strong>Isotype</strong></td> <td><div>IgG</div></td> </tr><tr class="lined"> <td><strong>Clonality</strong></td> <td><div>Polyclonal</div></td> </tr><tr class="lined"> <td><strong>Host</strong></td> <td><div>Goat</div></td> </tr><tr class="lined"> <td><strong>Gene</strong></td> <td><div>SMAD2</div></td> </tr><tr class="lined"> <td><strong>Purity Statement</strong></td> <td><div>Antigen Affinity-purified</div></td> </tr><tr class="lined"> <td><strong>Innovator's Reward</strong></td> <td><div>Test in a species/application not listed above to receive a full credit towards a future purchase.<br/><div class='text_right'><a class='ds_submit_blue ds_regularPad' href='/support/innovators-reward.html'>Learn about the Innovator's Reward</a></div></div></td> </tr> </table> </div> <h2 class="greyHead2">Applications/Dilutions</h2> <div class="ds_info"> <a id="tab1appsdiluts"></a> <table class="ds_list wide"> <tr class="lined"> <td><strong>Dilutions</strong></td> <td> <ul class="ds_noDotList"><li>Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells</li><li>Immunocytochemistry 5-15 ug/mL</li><li>Immunohistochemistry 3-15 ug/mL</li><li>Simple Western 25 ug/mL</li><li>Western Blot 0.5 ug/mL</li> </ul> </td> </tr><tr class="lined"> <td><strong>Reviewed Applications</strong></td> <td> <table class="wide"> <tr> <td> Read <a href="#ReviewsSection">2 Reviews</a> rated 4.5<div class="dsSprite_stars5"></div> using <br/>AF3797 in the following applications:<br /><br /> <ul class="information-list"><li> <div class="information-subject"><a href="#ReviewsSection" class="filter_link" data-destination="review" data-type="application" data-filter="Western Blot">Western Blot</a></div> <div class="information-data"></div> </li> </ul> </td> </tr> </table> </td> </tr><tr class="lined"> <td><strong>Publications</strong></td> <td> <table> <tr> <td>Read <a href="#PublicationSection"> Publications</a> using<br/> AF3797 in the following applications:<br /><br /> <ul class="information-list"><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ChIP">ChIP</a> </div><div class="information-data">4 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Flow Cytometry">Flow Cytometry</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="ICC">ICC</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="IHC">IHC</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Immunoprecipitation">Immunoprecipitation</a> </div><div class="information-data">2 publications</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Simple Western">Simple Western</a> </div><div class="information-data">1 publication</div></li><li class="noIndent"><div class="information-subject"> <a href="#PublicationSection" class="filter_link" data-destination="publication" data-type="application" data-filter="Western Blot">Western Blot</a> </div><div class="information-data">8 publications</div></li></ul></td> </tr> </table> </td> </tr> </table> </div> <h2 class="greyHead2">Packaging, Storage & Formulations</h2><div class="ds_info"><table class="ds_list"><tr class="lined"> <td><strong>Storage</strong></td> <td><div><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></div></td> </tr><tr class="lined"> <td><strong>Buffer</strong></td> <td><div>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.</div></td> </tr><tr class="lined"> <td><strong>Preservative</strong></td> <td><div>No Preservative</div></td> </tr><tr class="lined"> <td><strong>Concentration</strong></td> <td><div>LYOPH</div></td> </tr><tr class="lined"> <td><strong>Reconstitution Instructions</strong></td> <td><div>Reconstitute at 0.2 mg/mL in sterile PBS.</div></td> </tr></table></div> <h2 class="greyHead2">Notes</h2><div class="ds_info"> <a id="tab1notes"></a> <div>This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.</div> </div><h2 class="greyHead2">Alternate Names for Smad2/3 Antibody [Unconjugated]</h2> <div class="ds_info"> <a id="tab1altnames"></a> <div class="information-list"> <ul><li>hMAD-2</li><li>hSMAD2</li><li>JV18</li><li>JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)</li><li>MAD homolog 2</li><li>Mad protein homolog</li><li>Mad, mothers against decapentaplegic homolog 2</li><li>MADH2MGC34440</li><li>MADR2MGC22139</li><li>Mad-related protein 2</li><li>mother against DPP homolog 2</li><li>mothers against decapentaplegic homolog 2</li><li>Mothers against DPP homolog 2</li><li>Sma- and Mad-related protein 2</li><li>SMAD 2</li><li>SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)</li><li>SMAD, mothers against DPP homolog 2</li><li>Smad2</li><li>Smad2/3</li></ul></div> </div> <h2 class="greyHead2">Background</h2> <div class="ds_info"> <a id="tab1background"></a> <div><p>Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.</p></div> </div> <h2 class="greyHead2">Limitations</h2> <div class="ds_info"> <a id="tab1background"></a> <div>This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are <a href="/support/quality-assurance.html">guaranteed</a> for 1 year from date of receipt.</div> </div><h2 class="greyHead2">Customers Who Viewed This Item Also Viewed...</h2> <a id="custalsoviewed"></a><div id="cav_prev"><div class="sprite_leftArrow"></div></div> <div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout=0 data-cycle-prev="#cav_prev" data-cycle-next="#cav_next" data-cycle-slides="div.ds_cav_group" id="ds_customer_also_viewed"><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NB100-56479"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad3-antibody_nb100-56479" style="overflow: hidden; width: 280px;" title="Smad3 Antibody - BSA Free">Smad3 Antibody -<br> BSA Free</a>    </div><div class="sub-header">NB100-56479</div> <div class="images_customers"><div id="ss_prev_cav0" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev_cav0" data-cycle-next="#ss_next_cav0" data-cycle-slides="div.big_thumb_cust_container" data-index=0 ><div class="big_thumb_cust_container"> <a href="/products/smad3-antibody_nb100-56479" title="Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Smad3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56479-img0006.jpg" alt="Immunocytochemistry/ Immunofluorescence Smad3 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad3-antibody_nb100-56479" title="Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Smad3-Antibody-Western-Blot-NB100-56479-img0008.jpg" alt="Western Blot Smad3 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad3-antibody_nb100-56479" title="Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL." class="cust_viewed_0" > <img loading="lazy" src="https://images.novusbio.com/images/Smad3-Antibody-Immunohistochemistry-Paraffin-NB100-56479-img0004.jpg" alt="Immunohistochemistry-Paraffin Smad3 Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav0" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-0" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/smad3-antibody_nb100-56479#ReviewsSection"> <div class="sprite_3Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/smad3-antibody_nb100-56479#PublicationSection"> <div class="sprite_pubBook"></div> (8 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ca, Hu, Mu, Po, Pm, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-P, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF2097"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad4-antibody_af2097" style="overflow: hidden; width: 280px;" title="Smad4 Antibody [Unconjugated]">Smad4 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF2097</div> <div class="images_customers"><div id="ss_prev_cav1" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-1" data-cycle-prev="#ss_prev_cav1" data-cycle-next="#ss_next_cav1" data-cycle-slides="div.big_thumb_cust_container" data-index=1 ><div class="big_thumb_cust_container"> <a href="/products/smad4-antibody_af2097" title="Jurkat human acute T cell leukemia cell line treated with 10&amp;nbsp;ng/mL Recombinant Human IL‑12 (Catalog&amp;nbsp;# 219-IL) overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad4/DNA complexes were immunoprecipitated using 5&amp;nbsp;μg Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) or control antibody (Catalog&amp;nbsp;# AB‑108‑C) for 15&amp;nbsp;minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# BAF109). Immunocomplexes were captured using 50&amp;nbsp;μL of MagCellect Streptavidin Ferrofluid (Catalog&amp;nbsp;# MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad4_AF2097_Chromatin_Immunoprecipitation_11225.jpg" alt="Chromatin Immunoprecipitation (ChIP) Smad4 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad4-antibody_af2097" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad4_AF2097_Western_Blot_17168.jpg" alt="Western Blot Smad4 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad4-antibody_af2097" title="Smad4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="cust_viewed_1" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad4_AF2097_Immunocytochemistry__Immunofluorescence_17807.jpg" alt="Immunocytochemistry Smad4 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/smad4-antibody_af2097#PublicationSection"> <div class="sprite_pubBook"></div> (9 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ChIP, ICC, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB2029"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad7-antibody-293039_mab2029" style="overflow: hidden; width: 280px;" title="Smad7 Antibody (293039) [Unconjugated]">Smad7 Antibody<br> (293039) [Unconjugate</a>    </div><div class="sub-header">MAB2029</div> <div class="images_customers"><div id="ss_prev_cav2" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev_cav2" data-cycle-next="#ss_next_cav2" data-cycle-slides="div.big_thumb_cust_container" data-index=2 ><div class="big_thumb_cust_container"> <a href="/products/smad7-antibody-293039_mab2029" title="Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad7_MAB2029_Western_Blot_6004.jpg" alt="Western Blot Smad7 Antibody (293039) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad7-antibody-293039_mab2029" title="Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 &amp;micro;g/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 &amp;deg;C. Tissue was stained with the Anti-Mouse HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad7_MAB2029_Immunohistochemistry_6962.jpg" alt="Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad7-antibody-293039_mab2029" title="Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_2" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad7_MAB2029_Immunohistochemistry_9846.jpg" alt="Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/smad7-antibody-293039_mab2029#ReviewsSection"> <div class="sprite_3Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/smad7-antibody-293039_mab2029#PublicationSection"> <div class="sprite_pubBook"></div> (59 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="7754-BH/CF"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-tgf-beta-1-human-cell-expressed-cf_7754-bh-cf" style="overflow: hidden; width: 280px;" title="TGF-beta 1 [Unconjugated]">TGF-beta 1<br> [Unconjugated]</a>    </div><div class="sub-header">7754-BH/CF</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev_cav3" data-cycle-next="#ss_next_cav3" data-cycle-slides="div.big_thumb_cust_container" data-index=3 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-tgf-beta-1-human-cell-expressed-cf_7754-bh-cf" title="Recombinant human TGF-beta 1 (7754-BH/CF) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL." class="cust_viewed_3" > <img loading="lazy" src="https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg" alt="Bioactivity TGF-beta 1 [Unconjugated]" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-tgf-beta-1-human-cell-expressed-cf_7754-bh-cf#PublicationSection"> <div class="sprite_pubBook"></div> (53 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF3025"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/tgf-beta-ri-alk-5-antibody_af3025" style="overflow: hidden; width: 280px;" title="TGF-beta RI/ALK-5 Antibody [Unconjugated]">TGF-beta RI/ALK-5<br> Antibody [Unconjugate ...</a>    </div><div class="sub-header">AF3025</div> <div class="images_customers"><div id="ss_prev_cav4" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev_cav4" data-cycle-next="#ss_next_cav4" data-cycle-slides="div.big_thumb_cust_container" data-index=4 ><div class="big_thumb_cust_container"> <a href="/products/tgf-beta-ri-alk-5-antibody_af3025" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 &amp; 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/TGFbeta_RI_AF3025_Western_Blot_17081.jpg" alt="Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tgf-beta-ri-alk-5-antibody_af3025" title="TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images2/TGF-beta_RI_AF3025_Immunocytochemistry_9948.jpg" alt="Immunocytochemistry TGF-beta RI/ALK-5 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/tgf-beta-ri-alk-5-antibody_af3025" title="Myostatin signaling in longissimus dorsi muscle of low-birth-weight (LBWT) and normal-birth-weight (NBWT) neonatal pigs. (A): Representative western blots from four pairs of LBWT and NBWT pigs (N, NBWT; L, LBWT). (B–G): Protein abundance of myostain, ALK5 and ActRIIB, and protein abundance phosphorylation of smad2/3. Abundance was normalized to alpha -tubulin and phosphorylation normalized to the corresponding non-phospho-proteins. Results are means ± SE. n = 12. Values with different letters differ significantly (P ≤ 0.05). Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fphys.2017.00482/full), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_4" > <img loading="lazy" src="https://images.novusbio.com/images/af3025_human-tgf-beta-ri-alk-5-affinity-purified-polyclonal-ab-41202412402510.jpg" alt="Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/tgf-beta-ri-alk-5-antibody_af3025#PublicationSection"> <div class="sprite_pubBook"></div> (16 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF1230"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk2-antibody_af1230" style="overflow: hidden; width: 280px;" title="ERK2 Antibody [Unconjugated]">ERK2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF1230</div> <div class="images_customers"><div id="ss_prev_cav5" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev_cav5" data-cycle-next="#ss_next_cav5" data-cycle-slides="div.big_thumb_cust_container" data-index=5 ><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg" alt="Western Blot ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title=" ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. " class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg" alt="Immunohistochemistry ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk2-antibody_af1230" title="Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_5" > <img loading="lazy" src="https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg" alt="Knockout Validated ERK2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk2-antibody_af1230#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> IHC, KO, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-22203"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/erk1-antibody-1e5_nbp2-22203" style="overflow: hidden; width: 280px;" title="ERK1 Antibody (1E5) - BSA Free">ERK1 Antibody<br> (1E5) - BSA Free</a>    </div><div class="sub-header">NBP2-22203</div> <div class="images_customers"><div id="ss_prev_cav6" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev_cav6" data-cycle-next="#ss_next_cav6" data-cycle-slides="div.big_thumb_cust_container" data-index=6 ><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red)." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Flow-Cytometry-NBP2-22203-img0001.jpg" alt="Flow Cytometry ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg" alt="Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/erk1-antibody-1e5_nbp2-22203" title="Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining." class="cust_viewed_6" > <img loading="lazy" src="https://images.novusbio.com/images/ERK1-Antibody-1E5-Immunohistochemistry-Paraffin-NBP2-22203-img0003.jpg" alt="Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/erk1-antibody-1e5_nbp2-22203#PublicationSection"> <div class="sprite_pubBook"></div> (1 Publication</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Pm, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF2039"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad1-antibody_af2039" style="overflow: hidden; width: 280px;" title="Smad1 Antibody [Unconjugated]">Smad1 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF2039</div> <div class="images_customers"><div id="ss_prev_cav7" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev_cav7" data-cycle-next="#ss_next_cav7" data-cycle-slides="div.big_thumb_cust_container" data-index=7 ><div class="big_thumb_cust_container"> <a href="/products/smad1-antibody_af2039" title="Smad1 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad1_AF2039_Immunohistochemistry_10106.jpg" alt="Immunohistochemistry Smad1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad1-antibody_af2039" title="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Smad1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad1_AF2039_Western_Blot_5162.jpg" alt="Western Blot Smad1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/smad1-antibody_af2039" title="Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Smad1 at approximately 66&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system. " class="cust_viewed_7" > <img loading="lazy" src="https://images.novusbio.com/images2/Smad1_AF2039_Simple_Western_16677.jpg" alt="Simple Western Smad1 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/smad1-antibody_af2039#PublicationSection"> <div class="sprite_pubBook"></div> (8 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP3-35329"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/gars-antibody_nbp3-35329" style="overflow: hidden; width: 280px;" title="GARS Antibody">GARS Antibody</a>    </div><div class="sub-header">NBP3-35329</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev_cav8" data-cycle-next="#ss_next_cav8" data-cycle-slides="div.big_thumb_cust_container" data-index=8 ><div class="big_thumb_cust_container"> <a href="/products/gars-antibody_nbp3-35329" title="Western Blot: GARS Antibody [NBP3-35329] - Western blot analysis of various lysates using GARS Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s." class="cust_viewed_8" > <img loading="lazy" src="https://images.novusbio.com/images/nbp3-35329_rabbit-gars-pab-1012202411334018.jpg" alt="Western Blot GARS Antibody" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF660"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ctgf-ccn2-antibody_af660" style="overflow: hidden; width: 280px;" title="CTGF/CCN2 Antibody [Unconjugated] - C-terminus">CTGF/CCN2 Antibody<br> [Unconjugated] - C-te ...</a>    </div><div class="sub-header">AF660</div> <div class="images_customers"><div id="ss_prev_cav9" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev_cav9" data-cycle-next="#ss_next_cav9" data-cycle-slides="div.big_thumb_cust_container" data-index=9 ><div class="big_thumb_cust_container"> <a href="/products/ctgf-ccn2-antibody_af660" title="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg" alt="Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ctgf-ccn2-antibody_af660" title="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg" alt="Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ctgf-ccn2-antibody_af660" title="Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P &lt;0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&amp;D Systems." class="cust_viewed_9" > <img loading="lazy" src="https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg" alt="Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus" class="big_thumb" /> </a> </div></div><div id="ss_next_cav9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/ctgf-ccn2-antibody_af660#PublicationSection"> <div class="sprite_pubBook"></div> (7 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> IHC</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-91258"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/fibronectin-antibody_nbp1-91258" style="overflow: hidden; width: 280px;" title="Fibronectin Antibody - BSA Free">Fibronectin<br> Antibody - BSA Free</a>    </div><div class="sub-header">NBP1-91258</div> <div class="images_customers"><div id="ss_prev_cav10" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev_cav10" data-cycle-next="#ss_next_cav10" data-cycle-slides="div.big_thumb_cust_container" data-index=10 ><div class="big_thumb_cust_container"> <a href="/products/fibronectin-antibody_nbp1-91258" title="Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were intraperitoneally injected an hour before the surgery and daily thereafter. Representative images of Western blot of FN (fibronectin), alpha-SMA (alpha-smooth muscle actin) and GAPDH (loading control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30993884/) licensed under a CC-BY license." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Fibronectin Antibody - BSA Free-Western Blot-NBP1-91258-img0022.jpg" alt="Western Blot Fibronectin Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/fibronectin-antibody_nbp1-91258" title="Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0003.jpg" alt="Western Blot Fibronectin Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/fibronectin-antibody_nbp1-91258" title="Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate." class="cust_viewed_10" > <img loading="lazy" src="https://images.novusbio.com/images/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0004.jpg" alt="Western Blot Fibronectin Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/fibronectin-antibody_nbp1-91258#ReviewsSection"> <div class="sprite_4Stars"></div> (11 Reviews</a>) </div> <div class="tiny"> <a href="/products/fibronectin-antibody_nbp1-91258#PublicationSection"> <div class="sprite_pubBook"></div> (56 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt</div><div class="tiny"><strong>Applications:</strong> ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP2-29463"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/p21-cip1-cdkn1a-antibody-wa-1_nbp2-29463" style="overflow: hidden; width: 280px;" title="p21/CIP1/CDKN1A Antibody (WA-1)">p21/CIP1/CDKN1A<br> Antibody (WA-1)</a>    </div><div class="sub-header">NBP2-29463</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev_cav11" data-cycle-next="#ss_next_cav11" data-cycle-slides="div.big_thumb_cust_container" data-index=11 ><div class="big_thumb_cust_container"> <a href="/products/p21-cip1-cdkn1a-antibody-wa-1_nbp2-29463" title="Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1)." class="cust_viewed_11" > <img loading="lazy" src="https://images.novusbio.com/images/p21-CIP1-CDKN1A-Antibody-WA-1-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg" alt="Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)" class="big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/p21-cip1-cdkn1a-antibody-wa-1_nbp2-29463#PublicationSection"> <div class="sprite_pubBook"></div> (24 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Pm, Hu, Pm, Mu</div><div class="tiny"><strong>Applications:</strong> Flow, ICC/IF, IHC, IHC-P, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="NBP1-19773"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/serpin-e1-pai-1-antibody_nbp1-19773" style="overflow: hidden; width: 280px;" title="Serpin E1/PAI-1 Antibody - BSA Free">Serpin E1/PAI-1<br> Antibody - BSA Free</a>    </div><div class="sub-header">NBP1-19773</div> <div class="images_customers"><div id="ss_prev_cav12" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-12" data-cycle-prev="#ss_prev_cav12" data-cycle-next="#ss_next_cav12" data-cycle-slides="div.big_thumb_cust_container" data-index=12 ><div class="big_thumb_cust_container"> <a href="/products/serpin-e1-pai-1-antibody_nbp1-19773" title="Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Serpin-E1-PAI-1-Antibody-Western-Blot-NBP1-19773-img0005.jpg" alt="Western Blot Serpin E1/PAI-1 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/serpin-e1-pai-1-antibody_nbp1-19773" title="Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP1-19773] - PAI1/Serpine1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red)." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Serpin-E1-PAI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19773-img0006.jpg" alt="Immunocytochemistry/ Immunofluorescence Serpin E1/PAI-1 Antibody - BSA Free" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/serpin-e1-pai-1-antibody_nbp1-19773" title="Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas." class="cust_viewed_12" > <img loading="lazy" src="https://images.novusbio.com/images/Serpin-E1-PAI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-19773-img0007.jpg" alt="Immunohistochemistry-Paraffin Serpin E1/PAI-1 Antibody - BSA Free" class="big_thumb" /> </a> </div></div><div id="ss_next_cav12" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-12" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/serpin-e1-pai-1-antibody_nbp1-19773#ReviewsSection"> <div class="sprite_5Stars"></div> (2 Reviews</a>) </div> <div class="tiny"> <a href="/products/serpin-e1-pai-1-antibody_nbp1-19773#PublicationSection"> <div class="sprite_pubBook"></div> (32 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="3218-ND"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/recombinant-human-nodal-protein_3218-nd" style="overflow: hidden; width: 280px;" title="Nodal [Unconjugated]">Nodal<br> [Unconjugated]</a>    </div><div class="sub-header">3218-ND</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-13" data-cycle-prev="#ss_prev_cav13" data-cycle-next="#ss_next_cav13" data-cycle-slides="div.big_thumb_cust_container" data-index=13 ><div class="big_thumb_cust_container"> <a href="/products/recombinant-human-nodal-protein_3218-nd" title="Nodal [Unconjugated]" class="cust_viewed_13"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="Nodal [Unconjugated]" title="Nodal [Unconjugated]" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/recombinant-human-nodal-protein_3218-nd#PublicationSection"> <div class="sprite_pubBook"></div> (22 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> BA</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF467"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/ephb2-antibody_af467" style="overflow: hidden; width: 280px;" title="EphB2 Antibody [Unconjugated]">EphB2 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF467</div> <div class="images_customers"><div id="ss_prev_cav14" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-14" data-cycle-prev="#ss_prev_cav14" data-cycle-next="#ss_next_cav14" data-cycle-slides="div.big_thumb_cust_container" data-index=14 ><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title=" Simple Western lane view shows lysates of COLO&amp;nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&amp;nbsp;kDa (as indicated) using 20&amp;nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg" alt="Simple Western EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="Western blot shows 25 ng of Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) and Recombinant Mouse EphB2 Fc Chimera (Catalog # 467-B2). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg" alt="Western Blot EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/ephb2-antibody_af467" title="EphB2 was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_14" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg" alt="Immunocytochemistry EphB2 Antibody [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav14" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-14" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/ephb2-antibody_af467#ReviewsSection"> <div class="sprite_4Stars"></div> (5 Reviews</a>) </div> <div class="tiny"> <a href="/products/ephb2-antibody_af467#PublicationSection"> <div class="sprite_pubBook"></div> (63 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> CyTOF-ready, Flow, ICC, IHC, Simple Western, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB8930"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/c-jun-antibody-1018y_mab8930" style="overflow: hidden; width: 280px;" title="c-jun [p Ser63] Antibody (1018Y) [Unconjugated]">c-jun [p Ser63]<br> Antibody (1018Y) [Unc ...</a>    </div><div class="sub-header">MAB8930</div> <div class="images_customers"><div id="ss_prev_cav15" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-15" data-cycle-prev="#ss_prev_cav15" data-cycle-next="#ss_next_cav15" data-cycle-slides="div.big_thumb_cust_container" data-index=15 ><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-c-Jun (S63) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg" alt="Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="c-Jun phosphorylated at S63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with Anisomycin using Rabbit Anti-Human Phospho-c-Jun (S63) Monoclonal Antibody (Catalog # MAB8930) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg" alt="Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/c-jun-antibody-1018y_mab8930" title="Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV)&amp;nbsp;with a&amp;nbsp;30 minute recovery, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-c‑Jun&amp;nbsp;(S63)&amp;nbsp;at approximately 49&amp;nbsp;kDa (as indicated) using&amp;nbsp;5 μg/mL&amp;nbsp;of Rabbit Anti-Human Phospho-c‑Jun (S63) Monoclonal Antibody (Catalog # MAB8930) . This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." class="cust_viewed_15" > <img loading="lazy" src="https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg" alt="Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav15" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-15" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/c-jun-antibody-1018y_mab8930#ReviewsSection"> <div class="sprite_5Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/c-jun-antibody-1018y_mab8930#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu</div><div class="tiny"><strong>Applications:</strong> ICC, Simple Western, WB</div> </div> </div> </div></div><div class="ds_cav_group"> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="MAB17761"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/jnk1-antibody-228601_mab17761" style="overflow: hidden; width: 280px;" title="JNK1 Antibody (228601) [Unconjugated]">JNK1 Antibody<br> (228601) [Unconjugate</a>    </div><div class="sub-header">MAB17761</div> <div class="images_customers"><div id="ss_prev_cav16" class="cycle-pager"> <div class="pager_left_on"></div></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-16" data-cycle-prev="#ss_prev_cav16" data-cycle-next="#ss_next_cav16" data-cycle-slides="div.big_thumb_cust_container" data-index=16 ><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg" alt="Western Blot JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg" alt="Western Blot JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div><div class="big_thumb_cust_container"> <a href="/products/jnk1-antibody-228601_mab17761" title="JNK1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="cust_viewed_16" > <img loading="lazy" src="https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg" alt="Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]" class="big_thumb" /> </a> </div></div><div id="ss_next_cav16" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-16" class="cycle-circle-pager"></div></div> <div class="ds_info"> <div class="tiny"> <a href="/products/jnk1-antibody-228601_mab17761#ReviewsSection"> <div class="sprite_4Stars"></div> (1 Review</a>) </div> <div class="tiny"> <a href="/products/jnk1-antibody-228601_mab17761#PublicationSection"> <div class="sprite_pubBook"></div> (3 Publications</a>) </div><div class="tiny"><strong>Species:</strong> Hu, Mu, Rt</div><div class="tiny"><strong>Applications:</strong> ICC, WB</div> </div> </div> </div> <div class="ds_cav_block"> <div class="ds_cav_upper" data-catnums="AF3797"> <div class="name"> <div class="flourish_left"></div> <div class="flourish_right"></div> <a href="/products/smad2-3-antibody_af3797" style="overflow: hidden; width: 280px;" title="Smad2/3 Antibody [Unconjugated]">Smad2/3 Antibody<br> [Unconjugated]</a>    </div><div class="sub-header">AF3797</div> <div class="images_customers"><div class="one_image_l"></div><div class="cycle-slideshow" data-cycle-fx="fade" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-17" data-cycle-prev="#ss_prev_cav17" data-cycle-next="#ss_next_cav17" data-cycle-slides="div.big_thumb_cust_container" data-index=17 ><div class="big_thumb_cust_container"> <a href="/products/smad2-3-antibody_af3797" title="Smad2/3 Antibody [Unconjugated]" class="cust_viewed_17"> <img loading="lazy" src="/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png" alt="Smad2/3 Antibody [Unconjugated]" title="Smad2/3 Antibody [Unconjugated]" class=" big_thumb" /> </a> </div></div><div class="one_image"></div><div class="one_image_11"></div></div> <div class="ds_info"><div class="tiny"><strong>Species:</strong> Hu, Mu</div><div class="tiny"><strong>Applications:</strong> WB, Simple Western, IHC, ChIP, ICC/IF</div> </div> </div> </div></div> </div><div id="cav_next"><div class="sprite_rightArrow"></div></div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_reviews-publications"> <a id="reviews-publications"></a><a id="PublicationSection"></a><h2 class="greyHead2">Publications for Smad2/3 Antibody (AF3797)(24)</h2><div class="ds_info"> <div>We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.<br /><br />We have publications tested in 7 applications: ChIP, Flow Cytometry, ICC, IHC, Immunoprecipitation, Simple Western, Western Blot.<br /><br /><div class="align-right"> <button class="greybutton btn_width" name="novusreview" onclick="window.location='/publications'"; >Submit a Publication</button><br /> </div> </div><div> <div class="filterParent"> <div class="publications_filterList" id="pubapp"> <div class="blueHead">Filter By Application</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ChIP"> <div style="display:inline;">ChIP</div> (4)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Flow Cytometry"> <div style="display:inline;">Flow Cytometry</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="ICC"> <div style="display:inline;">ICC</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="IHC"> <div style="display:inline;">IHC</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Immunoprecipitation"> <div style="display:inline;">Immunoprecipitation</div> (2)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Simple Western"> <div style="display:inline;">Simple Western</div> (1)</div><div class="filter_line"> <input type="checkbox" value="application" class="publication_filterCheckbox" data-filter="Western Blot"> <div style="display:inline;">Western Blot</div> (8)</div><div class="filter_clear" data-type="publication" data-filter="application">All Applications</div></div> <div class="publications_filterList" id="pubspec"> <div class="blueHead">Filter By Species</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Human"> <div style="display:inline;">Human</div> (11)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (5)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Rat"> <div style="display:inline;">Rat</div> (1)</div><div class="filter_line"> <input type="checkbox" value="species" class="publication_filterCheckbox" data-filter="Xenopus"> <div style="display:inline;">Xenopus</div> (1)</div><div class="filter_clear" data-type="publication" data-filter="species">All Species</div></div> </div></div> <div class="clear"></div> <div id="publications_list">Showing Publications 1 - <span id="appcnt">10</span> of 24. <a class="pubs_showall">Show All 24 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a><table class="wide lightPad tablesorter" id="publications_list_table"> <thead> <tr> <th>Publications using AF3797</th> <th>Applications</th> <th>Species</th> </tr> </thead> <tbody><tr class="lined revfil firstten" data-application="ICC" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37725511" target="_blank" rel="nofollow"> Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC, Human) </a></td> <td valign="top">ICC</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37709746" target="_blank" rel="nofollow"> Mouti MA, Deng S, Pook M et al. KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics Nature communications 9/14/2023 [PMID: 37709746] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/37405760" target="_blank" rel="nofollow"> Yoshida M, Kokubun T, Sato K et al. DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-?/Smad Signaling Pathway Investigative ophthalmology & visual science 7/3/2023 [PMID: 37405760] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Simple Western" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36835384" target="_blank" rel="nofollow"> RA Razali, MD Yazid, A Saim, RBH Idrus, Y Lokanathan Approaches in Hydroxytyrosol Supplementation on Epithelial-Mesenchymal Transition in TGFbeta1-Induced Human Respiratory Epithelial Cells International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835384] (Simple Western, Human) </a></td> <td valign="top">Simple Western</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/36143013" target="_blank" rel="nofollow"> M Sisto, D Ribatti, G Ingravallo, S Lisi The Expression of Follistatin-like 1 Protein Is Associated with the Activation of the EMT Program in Sjï¿½gren&#039;s Syndrome Journal of Clinical Medicine, 2022-09-13;11(18):. 2022-09-13 [PMID: 36143013] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/33793068" target="_blank" rel="nofollow"> Rebecca L. Mather, Abhijit Parolia, Sandra E. Carson, Erik Venalainen, David Roig‐Carles, Mustapha Jaber et al. The evolutionarily conserved long non‐coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms Molecular Oncology 7/1/2021 [PMID: 33793068] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32393780" target="_blank" rel="nofollow"> LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil firstten" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/32243809" target="_blank" rel="nofollow"> Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 4/2/2020 [PMID: 32243809] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil firstten" data-application="" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31947892" target="_blank" rel="nofollow"> L Wang, Y Li, X Wang, P Wang, K Essandoh, S Cui, W Huang, X Mu, Z Liu, Y Wang, T Peng, GC Fan GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype Cells, 2020-01-03;9(1):. 2020-01-03 [PMID: 31947892] (Mouse) </a></td> <td valign="top"></td> <td valign="top">Mouse</td> </tr><tr class="lined revfil firstten" data-application="Flow Cytometry, Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31925325" target="_blank" rel="nofollow"> M Sisto, L Lorusso, G Ingravallo, D Ribatti, S Lisi TGFbeta1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial-mesenchymal transition in Sjï¿½gren&#039;s syndrome Lab. Invest., 2020-01-10;100(6):824-836. 2020-01-10 [PMID: 31925325] (Flow Cytometry, Western Blot, Human) </a></td> <td valign="top">Flow Cytometry, Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31557150" target="_blank" rel="nofollow"> J Jang, D Han, M Golkaram, M Audouard, G Liu, D Bridges, S Hellander, A Chialastri, SS Dey, LR Petzold, KS Kosik Control over single-cell distribution of G1 lengths by WNT governs pluripotency PLoS Biol., 2019-09-26;17(9):e3000453. 2019-09-26 [PMID: 31557150] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ChIP, IHC" data-species="Xenopus"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31537794" target="_blank" rel="nofollow"> GE Gentsch, T Spruce, NDL Owens, JC Smith Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals Nat Commun, 2019-09-19;10(1):4269. 2019-09-19 [PMID: 31537794] (ChIP, IHC, Xenopus) </a></td> <td valign="top">ChIP, IHC</td> <td valign="top">Xenopus</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/31387632" target="_blank" rel="nofollow"> He R, Wang M, Zhao C et al. TFEB-driven autophagy potentiates TGF-beta induced migration in pancreatic cancer cells J. Exp. Clin. Cancer Res. 8/6/2019 [PMID: 31387632] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/30954335" target="_blank" rel="nofollow"> K Nakai, S Karita, J Igarashi, I Tsukamoto, K Hirano, Y Kubota COA-Cl prevented TGF-?1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis J. Dermatol. Sci., 2019-03-12;0(0):. 2019-03-12 [PMID: 30954335] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/29489750" target="_blank" rel="nofollow"> A Bertero, S Brown, P Madrigal, A Osnato, D Ortmann, L Yiangou, J Kadiwala, NC Hubner, IR de Los Moz, C Sadée, AS Lenaerts, S Nakanoh, R Grandy, E Farnell, J Ule, HG Stunnenber, S Mendjan, L Vallier The SMAD2/3 interactome reveals that TGF? controls m6A mRNA methylation in pluripotency Nature, 2018-02-28;555(7695):256-259. 2018-02-28 [PMID: 29489750] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="" data-species=""> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/29402304" target="_blank" rel="nofollow"> MS Ventura Fe, M Bienert, K Müller, B Rath, T Goecke, C Opländer, T Braunschwe, P Mela, TH Brümmendor, F Beier, S Neuss Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta Stem Cell Res Ther, 2018-02-05;9(1):28. 2/5/2018 [PMID: 29402304] </a></td> <td valign="top"></td> <td valign="top"></td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27939132" target="_blank" rel="nofollow"> Tao Cheng Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation Am. J. Pathol, 2016-12-08;0(0):. 2016-12-08 [PMID: 27939132] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/27501759" target="_blank" rel="nofollow"> Potential mechanisms underlying ectodermal differentiation of Wharton&#039;s jelly mesenchymal stem cells Biochem Biophys Res Commun, 2016-08-05;0(0):. 2016-08-05 [PMID: 27501759] (Western Blot, Human) </a></td> <td valign="top">Western Blot</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="ChIP" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/25775035" target="_blank" rel="nofollow"> Yoon , Se-Jin, Foley , Joseph W, Baker , Julie C HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun, 2015-03-16;6(0):6546. 2015-03-16 [PMID: 25775035] (ChIP, Mouse) </a></td> <td valign="top">ChIP</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="ChIP" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/24709321" target="_blank" rel="nofollow"> Rosa A, Papaioannou M, Krzyspiak J, Brivanlou A miR-373 is regulated by TGFbeta signaling and promotes mesendoderm differentiation in human Embryonic Stem Cells. Dev Biol, 2014-04-04;391(1):81-8. 2014-04-04 [PMID: 24709321] (ChIP, Human) </a></td> <td valign="top">ChIP</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Immunoprecipitation" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22912414" target="_blank" rel="nofollow"> Pereira P, Dobreva M, Maas E, Cornelis F, Moya I, Umans L, Verfaillie C, Camus A, de Sousa Lopes S, Huylebroeck D, Zwijsen A Antagonism of Nodal signaling by BMP/Smad5 prevents ectopic primitive streak formation in the mouse amnion. Development, 2012-09-01;139(18):3343-54. 2012-09-01 [PMID: 22912414] (Immunoprecipitation, Human) </a></td> <td valign="top">Immunoprecipitation</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="Western Blot" data-species="Mouse"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/22505452" target="_blank" rel="nofollow"> Kudryashova E, Kramerova I, Spencer MJ Satellite cell senescence underlies myopathy in a mouse model of limb-girdle muscular dystrophy 2H. J. Clin. Invest., 2012-04-16;122(5):1764-76. 2012-04-16 [PMID: 22505452] (Western Blot, Mouse) </a></td> <td valign="top">Western Blot</td> <td valign="top">Mouse</td> </tr><tr class="lined revfil hidden" data-application="ChIP" data-species="Human"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21741376" target="_blank" rel="nofollow"> Kim SW, Yoon SJ, Chuong E, Oyolu C, Wills AE, Gupta R, Baker J Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs. Dev. Biol., 2011-06-29;357(2):492-504. 2011-06-29 [PMID: 21741376] (ChIP, Human) </a></td> <td valign="top">ChIP</td> <td valign="top">Human</td> </tr><tr class="lined revfil hidden" data-application="IHC" data-species="Rat"> <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/17392319" target="_blank" rel="nofollow"> Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension. Eur. Respir. J., 2007-03-28;29(6):1094-104. 2007-03-28 [PMID: 17392319] (IHC, Rat) </a></td> <td valign="top">IHC</td> <td valign="top">Rat</td> </tr><tr><td colspan="3" align="center"> <a class="pubs_showall">Show All 24 Publications.</a> <a class="pubs_collapse" style="display:none;">Collapse Publications.</a> </td></tr></tbody> </table> </div> </div><a id="ReviewsSection"></a> <h2 class="greyHead2">Reviews for <span>Smad2/3 Antibody (AF3797)</span> (2) <span style="display:none;">4.5</span><span style="display:none;">2</span></h2> <div class="ds_info"> <div class="bottom15"> <strong>Average Rating: 4.5</strong> <div class="dsSprite_stars4"></div> (Based on 2 reviews) </div> <div><div class="ds_info">We have 2 reviews tested in 1 species: Mouse.</div> <div class="align-right"> <button class="greybutton btn_width" id="novusreview" name="novusreview" onclick="window.location='/reviews/AF3797'"; >Submit a Review</button><br /> </div> </div> <div class="blueHead"><strong>Reviews using AF3797:</strong></div> <div class="filterParent"><div class="reviews_filterList"> <div class="blueHead">Filter by Applications</div><div class="filter_line"> <input type="checkbox" value="application" class="review_filterCheckbox" data-filter="WB"> <div style="display:inline;">WB</div> (2)</div><div class="filter_clear" data-type="review" data-filter="application">All Applications</div></div> <div class="reviews_filterList"> <div class="blueHead">Filter by Species</div><div class="filter_line"> <input type="checkbox" value="species" class="review_filterCheckbox" data-filter="Mouse"> <div style="display:inline;">Mouse</div> (2)</div><div class="filter_clear" data-type="review" data-filter="species">All Species</div></div></div><div id="reviews_list"> <table class="wide lightPad tablesorter" id="reviews_list_table"> <thead> <tr> <th>Images</th> <th>Ratings</th> <th>Applications</th> <th>Species</th> <th>Date</th> <th>Details</th> </tr> </thead> <tbody><tr class="top-lined revfil" data-rating="Excellent" data-application="WB" data-species="Mouse"> <td><a href="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af3797_64431.jpg" title="Mouse cardiac fibroblast assessed for Smad2/3 expression" class="colorbox" title="Western Blot" > <img loading="lazy" src="https://www.novusbio.com/sites/novusbio.com/files/reviews/review_af3797_64431.jpg" class="reviews_imageBorder" width="75" alt="Western Blot Smad2/3 AF3797" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a><meta content="12/20/2024" /> </td> <td> <span style="display:none;">5</span> <div class="dsSprite_stars5" content="5"></div> <div class="revby">reviewed by:<br />KARTHIKEYAN THIRUGNANAM</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>12/20/2024</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>MOSUE CARDIAC fibroblasts</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr><tr><td class="text_bold">Lot</td><td>XHY0421071</td></tr></table> </div> </div> </td> </tr><tr class="top-lined revfil" data-rating="Very Good" data-application="WB" data-species="Mouse"> <td> <meta content="01/08/2015" /> </td> <td> <span style="display:none;">4</span> <div class="dsSprite_stars4" content="4"></div> <div class="revby">reviewed by:<br />Verified Customer</div> </td> <td title="Western Blot">WB</td> <td>Mouse</td> <td>01/08/2015</td> <td align="left"> <div class="dsSprite_expandReviews"><div class="expandReviewData">View</div></div> <div class="reviews_showMoreContent"> <div class="reviews_showMoreContentInner"><h2 class="greyHead">Summary</h2> <table class="even wide top"><tr><td class="text_bold">Application</td><td>Western Blot</td></tr><tr><td class="text_bold">Sample Tested</td><td>See PMID 22868230</td></tr><tr><td class="text_bold">Species</td><td>Mouse</td></tr></table> </div> </div> </td> </tr></tbody> </table> </div><br /> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_protocols-faqs"> <a id="protocols-faqs"></a><h2 class="greyHead2">Product General Protocols</h2> <a id="protocols"></a> <div class="ds_info">Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars. <ul><li><a href="/support/support-by-application/western-blot/protocol.html">Western Blot</a></li><li><a href="/support/support-by-application.html">View all Protocols, Troubleshooting, Illustrated assays and Webinars.</a></li> </ul></div><h2 class="greyHead2 ds_info">Video Protocols</h2> <a id="videos"></a> <div class="ds_info"><div class="movie-cell"> <span class="movie-title">WB Video Protocol</span><br/> <meta content ="https://www.youtube.com/watch?v=uFu8aie4QFI" /> <a href="https://www.youtube.com/embed/uFu8aie4QFI?rel=0&wmode=transparent" class="youtube"> <div class="reduce sprite_WBvideo"></div> </a> </div></div> <div class="clear"></div><h2 class="greyHead2">FAQs for Smad2/3 Antibody (AF3797) (0)</h2> <a id="faqs"></a> <div class="ds_info">There are no specific FAQs related to this product. Read our general <a href="/support/faqs.html">customer & technical service FAQs.</a> </div> <div class="clear"></div></div> <div class="ds_tab_content equalbottom" id="content_supportresearch"> <a id="supportresearch"></a><table cellpadding="0" cellspacing="0"><tr><td valign="top" class="width50"> <h2 class="greyHead2">Secondary Antibodies</h2> <a id="secondaryantibodies"></a> <div class="ds_info"> <ul class="information-list"><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=HRP">Anti-Goat HRP Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=FITC">Anti-Goat FITC Labeled Antibodies</a></li><li><a href="/product-type/secondary-antibodies?species=Goat&facet_conjugate=Biotin">Anti-Goat Biotin Labeled Antibodies</a></li><li> </li> <li><a href="/product-type/secondary-antibodies#fq=species%3A%22Goat%22">View all of our anti-Goat Secondary Antibodies</a></li> </ul> </div></td><td width="5"> </td><td valign="top"> <h2 class="greyHead2" >Isotype Controls</h2> <a id="isotypecontrols"></a> <div class="ds_info"> <ul class="ds_noDotList"><li><a href="/product-type/isotype-controls#fq=species%3A%22Goat%22">Goat Isotype Controls</a></li><li> </li> <li><a href="/product-type/isotype-controls">View all of our Isotype Control Products</a></li> </div></td></tr></table><h2 class="greyHead2">Additional Smad2/3 Products</h2> <a id="addtlalphaprods"></a> <div class="ds_info"> <div> <span style="display:none;">Array AF3797</span> <ul class="ds_noDotList"><li> <a href="/primary-antibodies/smad2-3">Smad2/3 Antibodies</a></li></ul> </div> </div><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><table cellpadding="0" cellspacing="0" class="width100"><tr></tr></table><h2 class="greyHead2">Blogs on Smad2/3</h2> <a id="blogsect"></a> <div>There are no specific blogs for Smad2/3, but you can <a href="/antibody-news/">read our latest blog posts.</div> <div class="clear"></div></div> </div></div><div class="grid_6"><div class="ds_blue_border marTop26 equalbottom"><a id="jump_recommended"></a> <h2 class="greyHead2">Customers Who Bought This Also Bought</h2> <div class="text_center"><div class="images2"><div id="ss_prev_sidebar" class="cycle-prev ds_rp_prev"><div class="pager_left_on"></div></div><div id="ss_next_sidebar" class="cycle-next ds_rp_next"><div class="pager_right_on"></div></div><div class="cycle-slideshow" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-prev="#ss_prev_sidebar" data-cycle-next="#ss_next_sidebar" data-cycle-slides="div.big_thumb_container" id="ds_customer_who_bought" ><div class="big_thumb_container" data-catnums="AB-108-C"> <div class="img_container"> <a href="/products/igg-isotype-control_ab-108-c" title="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/IgG_AB108C_Control_19376.jpg" title="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." alt="SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog&nbsp;#AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog&nbsp;# AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog&nbsp;#F0107)." class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/igg-isotype-control_ab-108-c">Goat IgG Isotype Control [Unconjugated]</a> <br />AB-108-C</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/igg-isotype-control_ab-108-c';" >View Datasheet</button> </div> </div><div class="big_thumb_container" data-catnums="AF467"> <div class="img_container"> <a href="/products/ephb2-antibody_af467" title=" Simple Western lane view shows lysates of COLO&nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&nbsp;kDa (as indicated) using 20&nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " class="cust_bought" > <img loading="lazy" src="https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg" title=" Simple Western lane view shows lysates of COLO&nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&nbsp;kDa (as indicated) using 20&nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " alt=" Simple Western lane view shows lysates of COLO&nbsp;205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2&nbsp;mg/mL. A specific band was detected for EphB2 at approximately 139 and 146&nbsp;kDa (as indicated) using 20&nbsp;µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. " class="big_thumb" /> </a> </div> <div class="caption_container"><a href="/products/ephb2-antibody_af467">EphB2 Antibody [Unconjugated]</a> <br />AF467</div> <div style="text-align: right"><button class="greybutton" id="novusviewdatasheet" name="novusviewdatasheet" onclick="window.location='/products/ephb2-antibody_af467';" >View Datasheet</button> </div> </div></div></div></div><div class="clear"></div><a id="jump_distributor"></a> <h2 class="greyHead2">Contact Information</h2> <div class="ds_info"> <div id="distributors"></div> </div> <div id="et_reload_trigger" style="display:none;">reload</div><h2 class="greyHead2">Product PDFs</h2><div class="silverHeadShort"> <span class="file"><a href="//aero.bio-techne.com/sds/pdf/af3797" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//aero.bio-techne.com/sds/pdf/af3797" target="_blank" class="datasheet-download sdsFile">PDF SDS</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="//resources.rndsystems.com/pdfs/datasheets/af3797.pdf" target="_blank" class="sprite_dsPDF"><span class="pdfSmaller"> </span></a></span> <span class="download-file-text" ><a href="//resources.rndsystems.com/pdfs/datasheets/af3797.pdf" target="_blank" class="datasheet-download">PDF Datasheet</a></span> </div><div class="silverHeadShort"> <span class="file"><a href="mailto:novus@novusbio.com?subject=Request%20CofA%20for%20AF3797" class="sprite_newDocument"><span class="downloadBlank"> </span></a></span> <span class="download-file-text"><a href="mailto:nb-technical@bio-techne.com?subject=Request%20CofA%20for%20AF3797&body=Specific%20catalog%20number(if multiple sizes):%0ALot%20Number:%0AAny%20additional%20products:">Request CofA</a></span> </div><h2 class="greyHead2">Calculators</h2> <div class="calc_wrapper"> <div class="dsSprite_calc"></div> <div class="fake_link" id="calc_reconstitution_link">Concentration Calculator</div> <div id="calc_reconstitution_wrapper" class="calc_function_wrapper"> <div class="calc_popup"> <div class="calc_close"> <div class="dsSprite_calc_close"></div> </div> <div class="calc_header">Concentration Calculator</div> <div id="reconstitution_calculator" class="calculator"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="concentration" class="calculator_input calculator_text"/> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button"/> </div> </div> </div> </div> </div> <h2 class="greyHead2">Recent Reviews</h2><div class="review_summary"> <div class="review_summary_left"> <div class="review_large_number">4.5</div> <div class="dsSprite_stars4"></div> </div> <div class="review_summary_right"> <table> <tbody><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">5</div></td><td style="position:relative;width:155px;"><div class="review_bar star5" style="width:100%;"></div><div class="review_bar_value">1</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">4</div></td><td style="position:relative;width:155px;"><div class="review_bar star4" style="width:100%;"></div><div class="review_bar_value">1</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">3</div></td><td style="position:relative;width:155px;"><div class="review_bar star3" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">2</div></td><td style="position:relative;width:155px;"><div class="review_bar star2" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr><tr><td><div class="dsSprite_emptyStar"></div><div class="review_summary_star_number">1</div></td><td style="position:relative;width:155px;"><div class="review_bar star1" style="width:0%;"></div><div class="review_bar_value">0</div></td></tr> </tbody> </table> </div> <hr /><div class="single_review"><div class="review_summary_image"><a href="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af3797_64431.jpg" title="Mouse cardiac fibroblast assessed for Smad2/3 expression" class="colorbox" title="Western Blot" > <img loading="lazy" src="//www.novusbio.com/sites/novusbio.com/files/reviews/review_af3797_64431.jpg" class="reviews_imageBorder" width="75" alt="Western Blot Smad2/3 AF3797" /> <br/><span class="enlarge" href="#"><div class="dsSprite_magGlass"></div> Enlarge</span></a></div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars5" content="5"></div></td> <td>KARTHIKEYAN THIRUGNANAM<br />12/20/2024</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>WB<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Mouse</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="single_review"><div class="review_summary_image"> </div> <div class="review_summary_right"><table> <tbody> <tr> <td><div class="dsSprite_stars4" content="4"></div></td> <td>Verified Customer<br />01/08/2015</td> </tr> <tr> <td class="review_summary_title">Application: </td> <td>WB<td> </tr> <tr> <td class="review_summary_title">Species: </td> <td>Mouse</td> </tr> </tbody> </table> <div class="text_right" style="margin-bottom:-8px;"><a href="#ReviewsSection">read this review</a></div></div></div> <hr /><div class="align-right" style="margin-right:10px;"> <a href="#ReviewsSection"> <button class="greybutton btn_width">read all the reviews</button> </a> </div> </div><h2 class="greyHead2">Bioinformatics</h2> <div class="ds_info"> <a id="tab1bioinformatics"></a> <div> <table class="ds_list_right wide top"> <tr class="lined"> <td><strong>Gene Symbol</strong></td> <td><a href="/gene-name/SMAD2">SMAD2</a></td> </tr><tr class="grey-line-top"> <td><strong>Uniprot</strong></td> <td> <ul class="bioinf-list"><li><a target="_blank" href="http://www.uniprot.org/uniprot/?query=P84022" rel="nofollow"><div class="sprite_newWindow"> </div> P84022</a><span class="inline ">()</span></li> </ul> </td> </tr></table> </div> </div> </div> </div><div class="clear"></div><noscript><form autocomplete="off" action="/products/smad2-3-antibody_af3797" method="post" id="et-add-multiple-to-cart-form" accept-charset="UTF-8"><div><table class="sticky-enabled"> <thead><tr><th>Size</th><th>Price</th> </tr></thead> <tbody> <tr class="odd"><td><div class="atc_size" title="catalog # AF3797-SP">25 ug</div><div data-msg='' class="atc_catnum">AF3797-SP</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">8-10 Business Days</td> </tr> <tr class="odd"><td><div class="atc_size" title="catalog # AF3797">100 ug</div><div data-msg='' class="atc_catnum">AF3797</div></td><td><div style="font-size:11px;"><a class="jump_distributor" href="#jump_distributor">Contact Your<br />Distributor</a></div></td> </tr> <tr class="even"><td colspan="3" class="stock_row">4-6 Business Days</td> </tr> </tbody> </table> <input type="hidden" name="form_build_id" value="form-q7wUhlNkbF31yW163hP-Y2CBd7GR4PuSlzRYCqS5glQ" /> <input type="hidden" name="form_id" value="et_add_multiple_to_cart_form" /> </div></form></noscript> </div> </div> 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Smad2/3 Antibody [Unconjugated] (AF3797): Novus Biologicals