Ready-to-Use Cytokines for Simplified Workflows

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paragraph--view-mode--default"> <div><p><a href="#Available Proteins">Available Proteins</a> | <a href="#Options">Ready-to-Use Cytokine Options</a> | <a href="#data">Data & Specifications</a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/scaleready-logo-525x300.png" alt="ScaleReady logo" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h1>Cytokine Formats to Fit Your Needs</h1></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>To streamline your workflow and enable your program to reach the clinic faster, we now offer our best-selling immune cell therapy cytokines in three convenient ready-to-use formats. Select from a liquid formulation, a process-optimized single-use vial, or a closed-process bag with weldable tubing, customizing your reagent formats to precisely match your workflow requirements.</p><p>Offered in Partnership with ScaleReady. <a href="/research-areas/immune-cell-therapy/scaleready" target="_self"><strong>Learn more.</strong></a></p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>ProPak™ GMP Cytokines Now Available!</h2></div> <a href="/gmp-products/closed-process-solutions" > <button type="button" class="btn btn-primary"> Use in Your Closed Process </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Available Proteins"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2><a name="Available Proteins"></a>Ready-to-Use Animal-Free GMP and RUO Proteins </h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><tbody><tr><td rowspan="2"><strong>Protein Name</strong></td><td colspan="2"><strong>Liquid </strong></td><td colspan="2"><strong>Process-Sized</strong></td></tr><tr><td><strong>RUO Vial</strong></td><td><strong>GMP Vial</strong></td><td><strong>GMP Vial (Lyophilized)</strong></td><td><strong>GMP ProPak (Liquid in Bag)</strong></td></tr><tr><td>IL-2</td><td><a href="/p/proteins-enzymes/recombinant-human-il-2-protein-animal-free_bt-002-afl">50 μg, 1 mg</a></td><td><a href="/p/proteins-enzymes/recombinant-human-il-2-gmp-protein-cf_bt-002-gmp">50 μg, 1 mg</a></td><td> </td><td> </td></tr><tr><td>IL-7</td><td><a href="/p/proteins-enzymes/recombinant-human-il-7-protein-animal-free_bt-007-afl">25 μg</a></td><td><a href="/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp">25 μg</a></td><td><a href="/p/proteins-enzymes/recombinant-human-il-7-gmp-protein-cf_bt-007-gmp">10 µg</a></td><td><a href="/p/proteins-enzymes/propak-recombinant-human-il-7-gmp-protein-cf_ppk-007-gmp">10 µg</a></td></tr><tr><td>IL-15</td><td><a href="/p/proteins-enzymes/recombinant-human-il-15-protein-animal-free_bt-015-afl">25 μg</a></td><td><a href="/p/proteins-enzymes/recombinant-human-il-15-gmp-protein-cf_bt-015-gmp">25 μg</a></td><td><a href="/p/proteins-enzymes/recombinant-human-il-15-gmp-protein-cf_bt-015-gmp">10 µg</a></td><td><a href="/p/proteins-enzymes/propak-recombinant-human-il-15-gmp-protein-cf_ppk-015-gmp">10 µg</a></td></tr><tr><td>IL-21</td><td><a href="/p/proteins-enzymes/recombinant-human-il-21-protein-animal-free_bt-021-afl">25 μg, 1 mg</a></td><td><a href="/p/proteins-enzymes/recombinant-human-il-21-gmp-protein-cf_bt-021-gmp">25 μg, 1 mg</a></td><td> </td><td> </td></tr></tbody></table></figure><p>Liquid formulations for these proteins are currently available to ship to the United States, Canada, and Europe. We are working to expand shipping to other parts of the world.</p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Why Choose Liquid Cytokines"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Options"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Liquid Cytokines: Eliminate Reconstitution Steps </h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Designed to simplify your complex processes, liquid frozen cytokines streamline your workflow, simplify your protocol, and achieve greater research efficiencies by eliminating the reconstitution step. This removes the risk of errors associated with reconstitution plus eliminates the need to maintain a sterile reconstitution buffer. Simply thaw and add to your cell culture. </p><p>Protein stability is paramount to ensure safety, bioactivity, and minimal variability. With this in mind, we have optimized protein concentrations and conducted extensive stability testing. You can rest assured of the potency and effectiveness of the liquid cytokines over time, ensuring consistent performance and reliable outcomes for your cell therapy. </p></div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="process-sized-cytokines"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="request further information"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Process-Sized Cytokines: Eliminate Aliquoting Steps</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Process-sized vials remove the need to aliquot your cytokines, allowing for higher patient safety and reduced hands-on technician time. These single-use vials have been process-optimized and are compatible with any immune cell manufacturing system, including ScaleReady G-Rex<sup>®</sup> bioreactors. Simply use a syringe to reconstitute cytokines in the vial and add to your cell culture.  </p><ul><li>Simplify your cell manufacturing workflow</li><li>Reduce manual touchpoints</li><li>Eliminate aliquoting variability</li></ul></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/gmp-products/gmp-proteins/sample-gmp-proteins#form" class="btn btn-primary" type="link"> Request a Sample </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="dU34XnGY15E" params="rel=0" style="background-image: url('https://img.youtube.com/vi/dU34XnGY15E/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/dU34XnGY15E?autoplay=1" class="lty-playbtn" title="Single Use Cytokines | CGTI On-Demand Webinar"> <span class="lyt-visually-hidden">Single Use Cytokines | CGTI On-Demand Webinar</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>ProPak: The Benefits of Liquid and Process-Sized GMP Cytokines</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/propak-kit-img-v3.jpg" alt="Propak Kit Img" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>ProPak GMP cytokines offer a "best of both" approach: liquid cytokines in process sizes, with the additional benefit of weldable bags supporting a closed process workflow. GMP IL-7 and IL-15 are delivered through process-sized bags with weldable tubing and are compatible with bagged media for use in a 1 L bioreactor, such as the G-Rex.</p><p>Learn more about <a href="/gmp-products/closed-process-solutions">closed system solutions</a>.</p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="data"></a></p></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-cytokine-titration-data20" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Cytokine Titration Data</h5> </a> </div> <div class="collapse" id="expPara-cytokine-titration-data20" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/reagents/cd-127-and-t-cell-expansion-data-v2.png" alt="T Cell Expansion and CD127 in IL-15 data" loading="lazy" > </div> <br/> <div><p>In-house scientists determined the concentrations of IL-7 and IL-15 that yield the optimum balance of T cell expansion and memory phenotype. Based on these findings, we now offer process-sized cytokines, enabling you to generate your desired phenotype while requiring less protein and producing less waste. For a demonstration of a hands-free T cell expansion workflow, check out the <a href="/resources/literature/t-cell-expansion-g-rex-cytokine-fill-and-forget-workflow">G-Rex Fill-and-Forget Workflow Application Note</a>.  </p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-equivalent-bioactivity-between-liquid-and-lyophilized-cytokines3" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Equivalent Bioactivity Between Liquid and Lyophilized Cytokines</h5> </a> </div> <div class="collapse" id="expPara-equivalent-bioactivity-between-liquid-and-lyophilized-cytokines3" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/liquid-cytokines-IL-2-7-15-graphs-banner.png" alt="Liquid Cytokines IL-2, IL-7, IL-15 graphs" loading="lazy" > </div> <br/> <div><p>Direct comparisons of the liquid frozen (blue circles) and lyophilized (red squares) formulations of GMP IL-2, IL-7, and IL-15. All 3 cytokines were tested in cell proliferation assays with no difference in bioactivity observed for any of the cytokines. IL-2 was tested on CTLL-2 mouse cytotoxic T cells, IL-7 was tested on PHA-activated human peripheral blood lymphocytes, and IL-15 was tested on MO7e human megakaryocytic leukemic cells.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-equivalent-t-cell-expansion-and-phenotype-between-liquid-and-lyophilized-cytokines6" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Equivalent T Cell Expansion and Phenotype Between Liquid and Lyophilized Cytokines</h5> </a> </div> <div class="collapse" id="expPara-equivalent-t-cell-expansion-and-phenotype-between-liquid-and-lyophilized-cytokines6" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/graphs/liquid-and-lyophilized-cyotkines-graphs-v3.png" alt="Equivalent T Cell Expansion and Phenotype Between Liquid and Lyophilized Cytokines" loading="lazy" > </div> <br/> <div><p>Primary human T cell expansion was evaluated using both liquid format and lyophilized cytokines. Cells were expanded in G-Rex<sup>®</sup> 6M well plates using complete GMP Human T Cell Expansion Media supplemented with either 200 IU/mL GMP IL-2 or a combination of 10 ng/mL of both GMP IL-7 and GMP IL-15 in both liquid and lyophilized formats. After 9 days of expansion, cells were assessed for fold expansion and viability (left; data labels are mean % viability) as well as for memory phenotype using flow cytometry (right).</p></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Specifications"></a></p></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-specifications-of-liquid-and-lyophilized-cytokines7" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Specifications of Liquid and Lyophilized Cytokines</h5> </a> </div> <div class="collapse" id="expPara-specifications-of-liquid-and-lyophilized-cytokines7" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><thead><tr><th>IL-2</th><th>Liquid</th><th>Lyophilized</th></tr></thead><tbody><tr><th>Formulation    </th><td>0.50 mg/mL, 0.2 μm filtered solution in sodium acetate</td><td>Lyophilized from a 0.2 μm filtered solution in sodium acetate with Trehalose</td></tr><tr><th>Stability</th><td>A minimum of 6 months when stored at ≤ -65 °C as supplied                 </td><td>A minimum of 12 months when stored at ≤ -20 °C as supplied</td></tr><tr><th>Pack Size</th><td>50 μg, 1 mg</td><td>50 μg, 1 mg</td></tr></tbody></table></figure><p> </p><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><thead><tr><th>IL-7</th><th>Liquid</th><th>Lyophilized</th></tr></thead><tbody><tr><th>Formulation</th><td>0.50 mg/mL, 0.2 μm filtered solution in PBS</td><td>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose</td></tr><tr><th>Stability</th><td>A minimum of 6 months when stored at ≤ -65 °C as supplied</td><td>A minimum of 12 months when stored at ≤ -20 °C as supplied</td></tr><tr><th>Pack Size</th><td>10 µg bag (1L process size), 25 μg</td><td>10 µg (1L process size), 25 μg, 1 mg</td></tr></tbody></table></figure><p> </p><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><thead><tr><th>IL-15</th><th>Liquid</th><th>Lyophilized</th></tr></thead><tbody><tr><th>Formulation</th><td>0.50 mg/mL, 0.2 μm filtered solution in PBS</td><td>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose</td></tr><tr><th>Stability</th><td>A minimum of 6 months when stored at ≤ -65 °C as supplied</td><td>A minimum of 12 months when stored at ≤ -20 °C as supplied</td></tr><tr><th>Pack Size</th><td>10 µg bag (1L process size), 25 μg</td><td>10 µg (1L process size), 25 μg, 1 mg</td></tr></tbody></table></figure><p> </p><figure class="responsive-figure-table"><table class="table table-bordered overflow-auto"><thead><tr><th>IL-21</th><th>Liquid</th><th>Lyophilized</th></tr></thead><tbody><tr><th>Formulation</th><td>0.50 mg/mL, 0.2 μm filtered solution in PBS</td><td>Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose</td></tr><tr><th>Stability</th><td>A minimum of 6 months when stored at ≤ -65 °C as supplied</td><td>A minimum of 12 months when stored at ≤ -20 °C as supplied</td></tr><tr><th>Pack Size</th><td>25 μg, 1 mg</td><td>25 μg, 1 mg</td></tr></tbody></table></figure><p>Stability data for liquid frozen cytokines is available on request.</p></div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div class="col-xl-9 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