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})();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19299112" data-work_id="19299112" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19299112/Impact_of_fresh_cycle_variables_on_the_implantation_potential_of_cryopreserved_thawed_human_embryos">Impact of fresh-cycle variables on the implantation potential of cryopreserved-thawed human embryos</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To compare the clinical outcomes of cryopreservation according to the indications for freezing and the outcomes of the fresh cycle from which the embryos were derived. Retrospective study. Private assisted-reproduction unit. Three... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19299112" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To compare the clinical outcomes of cryopreservation according to the indications for freezing and the outcomes of the fresh cycle from which the embryos were derived. Retrospective study. Private assisted-reproduction unit. Three thousand, three hundred and sixty-eight embryos in 702 frozen-thawed embryo transfer (ET) cycles. Clinical outcomes of frozen-thawed ET cycles in which embryos were derived from fresh conception cycles (n = 436), from fresh nonconception cycles (n = 180), or where the fresh cycle was cancelled due to impending ovarian hyperstimulation syndrome (OHSS) (n = 86). Cryosurvival, clinical pregnancy, and implantation rates. Despite similar cryosurvival rates and the transfer of a similar number of embryos, significantly higher clinical pregnancy and implantation rates were obtained in the fresh-conception and OHSS groups. The implantation potential of frozen-thawed embryos that were not selected in a prior fresh cycle was comparable to that of embryos selected for fresh transfer. The success of frozen-thawed ET depends on the outcome of fresh ET and the indications for cryopreservation. Success is similar to that of fresh ET cycles in patients who had all their embryos frozen due to impending OHSS.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19299112" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8aeb898316847368782a595b6e8902e1" rel="nofollow" data-download="{"attachment_id":42099647,"asset_id":19299112,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42099647/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39554479" href="https://ku.academia.edu/BulentUrman">Bulent Urman</a><script data-card-contents-for-user="39554479" type="text/json">{"id":39554479,"first_name":"Bulent","last_name":"Urman","domain_name":"ku","page_name":"BulentUrman","display_name":"Bulent Urman","profile_url":"https://ku.academia.edu/BulentUrman?f_ri=19295","photo":"https://0.academia-photos.com/39554479/10883563/12145835/s65_bulent.urman.jpg"}</script></span></span></li><li class="js-paper-rank-work_19299112 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19299112"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19299112, container: ".js-paper-rank-work_19299112", }); 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Retrospective study. Private assisted-reproduction unit. Three thousand, three hundred and sixty-eight embryos in 702 frozen-thawed embryo transfer (ET) cycles. Clinical outcomes of frozen-thawed ET cycles in which embryos were derived from fresh conception cycles (n = 436), from fresh nonconception cycles (n = 180), or where the fresh cycle was cancelled due to impending ovarian hyperstimulation syndrome (OHSS) (n = 86). Cryosurvival, clinical pregnancy, and implantation rates. Despite similar cryosurvival rates and the transfer of a similar number of embryos, significantly higher clinical pregnancy and implantation rates were obtained in the fresh-conception and OHSS groups. The implantation potential of frozen-thawed embryos that were not selected in a prior fresh cycle was comparable to that of embryos selected for fresh transfer. The success of frozen-thawed ET depends on the outcome of fresh ET and the indications for cryopreservation. Success is similar to that of fresh ET cycles in patients who had all their embryos frozen due to impending OHSS.","downloadable_attachments":[{"id":42099647,"asset_id":19299112,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39554479,"first_name":"Bulent","last_name":"Urman","domain_name":"ku","page_name":"BulentUrman","display_name":"Bulent Urman","profile_url":"https://ku.academia.edu/BulentUrman?f_ri=19295","photo":"https://0.academia-photos.com/39554479/10883563/12145835/s65_bulent.urman.jpg"}],"research_interests":[{"id":10966,"name":"Turkey","url":"https://www.academia.edu/Documents/in/Turkey?f_ri=19295","nofollow":false},{"id":12426,"name":"Treatment 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href="https://www.academia.edu/13989676/Defending_the_Ethical_Permissibility_of_Cryopreservation">Defending the Ethical Permissibility of Cryopreservation</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13989676" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7128022f734217e8e6adad847b29c4d9" rel="nofollow" data-download="{"attachment_id":38174187,"asset_id":13989676,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen 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type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="62550" href="https://www.academia.edu/Documents/in/Pregnancy">Pregnancy</a><script data-card-contents-for-ri="62550" type="text/json">{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=67873167]'), work: {"id":67873167,"title":"Advances in cooled 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href="https://www.academia.edu/71251280/Cryopreservation_of_sperm_from_the_endangered_formosan_landlocked_salmon_">Cryopreservation of sperm from the endangered formosan landlocked salmon ()</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/71251280" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="213135454" 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Okuzawa</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-71251280'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-71251280').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_71251280 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="71251280"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 71251280, container: ".js-paper-rank-work_71251280", }); });</script></li><li class="js-percentile-work_71251280 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 71251280; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_71251280"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_71251280 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="71251280"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 71251280; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=71251280]").text(description); $(".js-view-count-work_71251280").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_71251280").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="71251280"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="11417" href="https://www.academia.edu/Documents/in/Population_Dynamics">Population Dynamics</a>, <script data-card-contents-for-ri="11417" type="text/json">{"id":11417,"name":"Population Dynamics","url":"https://www.academia.edu/Documents/in/Population_Dynamics?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="14076" href="https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy">Transmission Electron Microscopy</a>, <script data-card-contents-for-ri="14076" type="text/json">{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="23979" href="https://www.academia.edu/Documents/in/Endangered_Species">Endangered Species</a><script data-card-contents-for-ri="23979" type="text/json">{"id":23979,"name":"Endangered Species","url":"https://www.academia.edu/Documents/in/Endangered_Species?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=71251280]'), work: {"id":71251280,"title":"Cryopreservation of sperm from the endangered formosan landlocked salmon ()","created_at":"2022-02-12T14:09:52.124-08:00","url":"https://www.academia.edu/71251280/Cryopreservation_of_sperm_from_the_endangered_formosan_landlocked_salmon_?f_ri=19295","dom_id":"work_71251280","summary":null,"downloadable_attachments":[],"ordered_authors":[{"id":213135454,"first_name":"Hiromi","last_name":"Ohta","domain_name":"independent","page_name":"HiromiOhta","display_name":"Hiromi Ohta","profile_url":"https://independent.academia.edu/HiromiOhta?f_ri=19295","photo":"https://0.academia-photos.com/213135454/72092513/60550309/s65_hiromi.ohta.png"},{"id":215003118,"first_name":"K.","last_name":"Okuzawa","domain_name":"independent","page_name":"KOkuzawa","display_name":"K. Okuzawa","profile_url":"https://independent.academia.edu/KOkuzawa?f_ri=19295","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":11417,"name":"Population Dynamics","url":"https://www.academia.edu/Documents/in/Population_Dynamics?f_ri=19295","nofollow":false},{"id":14076,"name":"Transmission Electron Microscopy","url":"https://www.academia.edu/Documents/in/Transmission_Electron_Microscopy?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":23979,"name":"Endangered Species","url":"https://www.academia.edu/Documents/in/Endangered_Species?f_ri=19295","nofollow":false},{"id":43028,"name":"Genetic Diversity","url":"https://www.academia.edu/Documents/in/Genetic_Diversity?f_ri=19295"},{"id":43820,"name":"Theriogenology","url":"https://www.academia.edu/Documents/in/Theriogenology?f_ri=19295"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=19295"},{"id":64240,"name":"Androgenesis","url":"https://www.academia.edu/Documents/in/Androgenesis?f_ri=19295"},{"id":79084,"name":"Hybrid","url":"https://www.academia.edu/Documents/in/Hybrid?f_ri=19295"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=19295"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=19295"},{"id":112334,"name":"Methanol","url":"https://www.academia.edu/Documents/in/Methanol?f_ri=19295"},{"id":342920,"name":"Fertility Rate","url":"https://www.academia.edu/Documents/in/Fertility_Rate?f_ri=19295"},{"id":432622,"name":"DMA","url":"https://www.academia.edu/Documents/in/DMA?f_ri=19295"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation?f_ri=19295"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=19295"},{"id":897073,"name":"Sperm Motility","url":"https://www.academia.edu/Documents/in/Sperm_Motility?f_ri=19295"},{"id":2470834,"name":"Oncorhynchus","url":"https://www.academia.edu/Documents/in/Oncorhynchus?f_ri=19295"},{"id":3574523,"name":"High risk","url":"https://www.academia.edu/Documents/in/High_risk?f_ri=19295"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_56294640" data-work_id="56294640" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/56294640/Development_of_a_cryopreservation_procedure_using_aluminium_cryo_plates">Development of a cryopreservation procedure using aluminium cryo-plates</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A cryopreservation procedure using an aluminium cryo-plate was successfully developed using in vitro-grown Dalmatian chrysanthemum (Tanacetum cinerariifolium) shoot tips. Shoot cultures were cold-hardened at 5 degree C on MS medium... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_56294640" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A cryopreservation procedure using an aluminium cryo-plate was successfully developed using in vitro-grown Dalmatian chrysanthemum (Tanacetum cinerariifolium) shoot tips. Shoot cultures were cold-hardened at 5 degree C on MS medium containing 0.5 M sucrose over a period of 20 to 40 days. Shoot tips with basal plate (1.0-1.5 x 1.0 mm) were dissected from shoot cultures and precultured at 5 degree C for 2 days on MS medium containing 0.5 M sucrose. Precultured shoot tips were placed on aluminium cryo-plates (7 mm x 37 mm x 0.5 mm) with 10 wells (diameter 1.5 mm, depth 0.75 mm) and embedded in alginate gel. Osmoprotection was performed by immersing the cryo-plates for 30 or 60 min in 25 ml pipetting reservoirs filled with loading solution (2 M glycerol + 1.4 M sucrose). For dehydration, the loading solution was replaced with PVS 7M vitrification solution (30 percent glycerol, 19.5 percent ethylene glycol and 0.6 M sucrose in liquid MS basal medium), which was applied for 40 min. After ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/56294640" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9ce07aca4d1bd3c235d20b92fdaf923c" rel="nofollow" data-download="{"attachment_id":71751046,"asset_id":56294640,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/71751046/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51943208" href="https://independent.academia.edu/TariqRafique4">Tariq Rafique</a><script data-card-contents-for-user="51943208" type="text/json">{"id":51943208,"first_name":"Tariq","last_name":"Rafique","domain_name":"independent","page_name":"TariqRafique4","display_name":"Tariq Rafique","profile_url":"https://independent.academia.edu/TariqRafique4?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_56294640 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="56294640"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 56294640, container: ".js-paper-rank-work_56294640", }); });</script></li><li class="js-percentile-work_56294640 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 56294640; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_56294640"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_56294640 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="56294640"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 56294640; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=56294640]").text(description); $(".js-view-count-work_56294640").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_56294640").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="56294640"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="151" href="https://www.academia.edu/Documents/in/Cryobiology">Cryobiology</a>, <script data-card-contents-for-ri="151" type="text/json">{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="27497" href="https://www.academia.edu/Documents/in/Instrumentation">Instrumentation</a><script data-card-contents-for-ri="27497" type="text/json">{"id":27497,"name":"Instrumentation","url":"https://www.academia.edu/Documents/in/Instrumentation?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=56294640]'), work: {"id":56294640,"title":"Development of a cryopreservation procedure using aluminium cryo-plates","created_at":"2021-10-07T06:05:12.010-07:00","url":"https://www.academia.edu/56294640/Development_of_a_cryopreservation_procedure_using_aluminium_cryo_plates?f_ri=19295","dom_id":"work_56294640","summary":"A cryopreservation procedure using an aluminium cryo-plate was successfully developed using in vitro-grown Dalmatian chrysanthemum (Tanacetum cinerariifolium) shoot tips. Shoot cultures were cold-hardened at 5 degree C on MS medium containing 0.5 M sucrose over a period of 20 to 40 days. Shoot tips with basal plate (1.0-1.5 x 1.0 mm) were dissected from shoot cultures and precultured at 5 degree C for 2 days on MS medium containing 0.5 M sucrose. Precultured shoot tips were placed on aluminium cryo-plates (7 mm x 37 mm x 0.5 mm) with 10 wells (diameter 1.5 mm, depth 0.75 mm) and embedded in alginate gel. Osmoprotection was performed by immersing the cryo-plates for 30 or 60 min in 25 ml pipetting reservoirs filled with loading solution (2 M glycerol + 1.4 M sucrose). For dehydration, the loading solution was replaced with PVS 7M vitrification solution (30 percent glycerol, 19.5 percent ethylene glycol and 0.6 M sucrose in liquid MS basal medium), which was applied for 40 min. After ...","downloadable_attachments":[{"id":71751046,"asset_id":56294640,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51943208,"first_name":"Tariq","last_name":"Rafique","domain_name":"independent","page_name":"TariqRafique4","display_name":"Tariq Rafique","profile_url":"https://independent.academia.edu/TariqRafique4?f_ri=19295","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":27497,"name":"Instrumentation","url":"https://www.academia.edu/Documents/in/Instrumentation?f_ri=19295","nofollow":false},{"id":55258,"name":"Aluminum","url":"https://www.academia.edu/Documents/in/Aluminum?f_ri=19295"},{"id":121939,"name":"Technique","url":"https://www.academia.edu/Documents/in/Technique?f_ri=19295"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=19295"},{"id":448182,"name":"Vitrification","url":"https://www.academia.edu/Documents/in/Vitrification?f_ri=19295"},{"id":2150526,"name":"Shoot","url":"https://www.academia.edu/Documents/in/Shoot?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8679496" data-work_id="8679496" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/8679496/Glucokinase_activator_PSN_GK1_displays_enhanced_antihyperglycaemic_and_insulinotropic_actions">Glucokinase activator PSN-GK1 displays enhanced antihyperglycaemic and insulinotropic actions</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Aims/hypothesis We evaluated the insulinotropic and antihyperglycaemic actions of glucokinase activators (GKAs), especially through acute and subchronic studies in rodent diabetes models with... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_8679496" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Aims/hypothesis We evaluated the insulinotropic and antihyperglycaemic actions of glucokinase activators (GKAs), especially through acute and subchronic studies in rodent diabetes models with (2R)-2-(4-cyclopropanesulphonylphenyl)-N-(5-fluorothiazol-2-yl)-3-(tetrahydropyran-4-yl)propionamide (PSN-GK1), a novel and potent GKA. Materials and methods The action of PSN-GK1 on or in the following were investigated: (1) on human liver glucokinase, insulin secretion from MIN6 cells and 2-deoxy-d-[3H]glucose (2-DG) uptake into rat hepatocytes; and (2) in Zucker diabetic fatty rats and in non-diabetic C57Bl/6, diabetic db/db and ob/ob mice. Results At 5 mmol/l glucose, PSN-GK1 activated glucokinase (4.3-fold, median effective concentration [EC50] 130 nmol/l), increased MIN6 insulin secretion (26-fold, EC50 267 nmol/l) and 2-DG hepatocytic uptake (threefold, EC50 1 μmol/l); at higher glucose concentrations, EC50s and fold-effectiveness were both lower. In C57Bl/6 mice, PSN-GK1 reduced blood glucose at 1 and 10 mg/kg (by mouth), but insulin was increased significantly at only the higher dose. In hyperinsulinaemic 10-mmol/l glucose clamps, PSN-GK1 increased 2-DG incorporation into liver glycogen sixfold, directly demonstrating liver effects. PSN-GK1 improved glycaemic profiles in db/db mice and Zucker diabetic fatty rats, diabetic animal models in which GKA efficacy has not previously been described, without causing hypoglycaemia. In ob/ob mice, it dose-dependently reduced excursions in OGTTs. Moreover, after subchronic administration, no tachyphylaxis was evident and glycaemia was improved without alterations to lipid levels, liver weight, glycogen content or body weight. Conclusions/interpretation PSN-GK1 was potently antihyperglycaemic through its effects on insulin release and hepatic glucose metabolism. It is one of the most potent GKAs described in the literature and is active in diabetic animal models where GKAs have not been reported to show efficacy to date. Ongoing human trials are investigating the potential of this novel therapeutic approach.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/8679496" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="679f3e5af2c3bebb3620dd1fb6b66a00" rel="nofollow" data-download="{"attachment_id":48021801,"asset_id":8679496,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48021801/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="18168342" href="https://independent.academia.edu/ChatfieldRichard">Richard Chatfield</a><script data-card-contents-for-user="18168342" type="text/json">{"id":18168342,"first_name":"Richard","last_name":"Chatfield","domain_name":"independent","page_name":"ChatfieldRichard","display_name":"Richard Chatfield","profile_url":"https://independent.academia.edu/ChatfieldRichard?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_8679496 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="8679496"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 8679496, container: ".js-paper-rank-work_8679496", }); 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Materials and methods The action of PSN-GK1 on or in the following were investigated: (1) on human liver glucokinase, insulin secretion from MIN6 cells and 2-deoxy-d-[3H]glucose (2-DG) uptake into rat hepatocytes; and (2) in Zucker diabetic fatty rats and in non-diabetic C57Bl/6, diabetic db/db and ob/ob mice. Results At 5 mmol/l glucose, PSN-GK1 activated glucokinase (4.3-fold, median effective concentration [EC50] 130 nmol/l), increased MIN6 insulin secretion (26-fold, EC50 267 nmol/l) and 2-DG hepatocytic uptake (threefold, EC50 1 μmol/l); at higher glucose concentrations, EC50s and fold-effectiveness were both lower. In C57Bl/6 mice, PSN-GK1 reduced blood glucose at 1 and 10 mg/kg (by mouth), but insulin was increased significantly at only the higher dose. In hyperinsulinaemic 10-mmol/l glucose clamps, PSN-GK1 increased 2-DG incorporation into liver glycogen sixfold, directly demonstrating liver effects. PSN-GK1 improved glycaemic profiles in db/db mice and Zucker diabetic fatty rats, diabetic animal models in which GKA efficacy has not previously been described, without causing hypoglycaemia. In ob/ob mice, it dose-dependently reduced excursions in OGTTs. Moreover, after subchronic administration, no tachyphylaxis was evident and glycaemia was improved without alterations to lipid levels, liver weight, glycogen content or body weight. Conclusions/interpretation PSN-GK1 was potently antihyperglycaemic through its effects on insulin release and hepatic glucose metabolism. It is one of the most potent GKAs described in the literature and is active in diabetic animal models where GKAs have not been reported to show efficacy to date. Ongoing human trials are investigating the potential of this novel therapeutic approach.","downloadable_attachments":[{"id":48021801,"asset_id":8679496,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":18168342,"first_name":"Richard","last_name":"Chatfield","domain_name":"independent","page_name":"ChatfieldRichard","display_name":"Richard Chatfield","profile_url":"https://independent.academia.edu/ChatfieldRichard?f_ri=19295","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=19295","nofollow":false},{"id":71300,"name":"Blood 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Model","url":"https://www.academia.edu/Documents/in/Animal_Model?f_ri=19295"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=19295"},{"id":336223,"name":"Digestive System","url":"https://www.academia.edu/Documents/in/Digestive_System?f_ri=19295"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=19295"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=19295"},{"id":432593,"name":"Hepatocytes","url":"https://www.academia.edu/Documents/in/Hepatocytes?f_ri=19295"},{"id":453923,"name":"Oral Hypoglycemic Agents","url":"https://www.academia.edu/Documents/in/Oral_Hypoglycemic_Agents?f_ri=19295"},{"id":564878,"name":"Body Weight","url":"https://www.academia.edu/Documents/in/Body_Weight?f_ri=19295"},{"id":806503,"name":"Thiazoles","url":"https://www.academia.edu/Documents/in/Thiazoles?f_ri=19295"},{"id":1490737,"name":"Secretion","url":"https://www.academia.edu/Documents/in/Secretion?f_ri=19295"},{"id":1681550,"name":"Psn","url":"https://www.academia.edu/Documents/in/Psn?f_ri=19295"},{"id":1953384,"name":"Activator","url":"https://www.academia.edu/Documents/in/Activator?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21120240" data-work_id="21120240" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21120240/Histological_identification_of_purified_and_cryopreserved_allogeneic_hepatocytes_following_transplantation_in_a_murine_model_without_host_immunosuppression">Histological identification of purified and cryopreserved allogeneic hepatocytes following transplantation in a murine model without host immunosuppression</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21120240" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="74f3186f636f6949872b4c496031d775" rel="nofollow" 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type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="31837" href="https://www.academia.edu/Documents/in/Cell_separation">Cell separation</a>, <script data-card-contents-for-ri="31837" type="text/json">{"id":31837,"name":"Cell separation","url":"https://www.academia.edu/Documents/in/Cell_separation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71437" href="https://www.academia.edu/Documents/in/Liver">Liver</a><script data-card-contents-for-ri="71437" type="text/json">{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21120240]'), work: {"id":21120240,"title":"Histological identification of purified and 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Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":31837,"name":"Cell separation","url":"https://www.academia.edu/Documents/in/Cell_separation?f_ri=19295","nofollow":false},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=19295","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=19295"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=19295"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=19295"},{"id":155901,"name":"Liver Transplantation","url":"https://www.academia.edu/Documents/in/Liver_Transplantation?f_ri=19295"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=19295"},{"id":416442,"name":"Transplant","url":"https://www.academia.edu/Documents/in/Transplant?f_ri=19295"},{"id":790533,"name":"Liver Failure","url":"https://www.academia.edu/Documents/in/Liver_Failure?f_ri=19295"},{"id":951639,"name":"Murine Model","url":"https://www.academia.edu/Documents/in/Murine_Model?f_ri=19295"},{"id":1163292,"name":"Survival Time","url":"https://www.academia.edu/Documents/in/Survival_Time?f_ri=19295"},{"id":1663252,"name":"Portal vein","url":"https://www.academia.edu/Documents/in/Portal_vein?f_ri=19295"},{"id":1740532,"name":"Acute rejection","url":"https://www.academia.edu/Documents/in/Acute_rejection?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_68163752 coauthored" data-work_id="68163752" itemscope="itemscope" 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class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66336288/Effect_of_Polyvinylpyrrolidone_on_Vitrification_of_Buffalo_Bubalus_bubalis_Oocytes">Effect of Polyvinylpyrrolidone on Vitrification of Buffalo (Bubalus bubalis) Oocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_66336288" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Vitrification, a method of rapid cooling, is an alternate cryopreservation method of oocytes and embryos. The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66336288" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="41faa09c98497e3a00663447cae98a72" rel="nofollow" data-download="{"attachment_id":77569374,"asset_id":66336288,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77569374/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="211349963" href="https://independent.academia.edu/lifescienceglobal">Lifescience Global Canada</a><script data-card-contents-for-user="211349963" type="text/json">{"id":211349963,"first_name":"Lifescience","last_name":"Global Canada","domain_name":"independent","page_name":"lifescienceglobal","display_name":"Lifescience Global Canada","profile_url":"https://independent.academia.edu/lifescienceglobal?f_ri=19295","photo":"https://0.academia-photos.com/211349963/71416544/59860660/s65_lifescience.global_canada.jpg"}</script></span></span></li><li class="js-paper-rank-work_66336288 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="66336288"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 66336288, container: ".js-paper-rank-work_66336288", }); 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The present study was aimed to examine the effect of polyvinylpyrrolidone (PVP) on vitrification of buffalo oocytes. Cumulus oocyte complexes (COCs) with fully grown oocytes (120-130 µm in diameter) were aspirated from slaughtered buffalo ovaries for vitrification. COCs were treated with equilibration solution at room temperature for 5 min and then transferred to a vitrification solution for 1 min. Then the COCs were submerged into liquid nitrogen (-196C) for a while using cryotops. The COCs were thawed, diluted, and washed in a washing solution for 5 min, respectively. Vitrified oocytes were incubated for in vitro maturation (IVM) at 38.5C under an atmosphere of 5% CO2 in the air for 24 hrs. Cumulus cells surrounding the oocytes were removed mechanically, oocytes were fixed in acetic acid and ethanol, and stained with aceto-orcein to examine the meiotic stages of oocytes. The numbers of morphologically normal oocytes after vitrification were higher in 5% PVP than 0 and 10% PVP groups. A proportion of oocytes treated with 5% PVP reached the metaphase II (MII) stage while none of the oocytes from 0% and 10% PVP groupsdeveloped beyond anaphase I and metaphase I (MI) stages, respectively. These results show that PVP can be used as a cryoprotectant for the vitrification of buffalo oocytes.","downloadable_attachments":[{"id":77569374,"asset_id":66336288,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":211349963,"first_name":"Lifescience","last_name":"Global Canada","domain_name":"independent","page_name":"lifescienceglobal","display_name":"Lifescience Global Canada","profile_url":"https://independent.academia.edu/lifescienceglobal?f_ri=19295","photo":"https://0.academia-photos.com/211349963/71416544/59860660/s65_lifescience.global_canada.jpg"}],"research_interests":[{"id":140,"name":"Pharmacology","url":"https://www.academia.edu/Documents/in/Pharmacology?f_ri=19295","nofollow":false},{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":306948,"name":"In 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href="https://www.academia.edu/Documents/in/Life_Sciences">Life Sciences</a>, <script data-card-contents-for-ri="8014" type="text/json">{"id":8014,"name":"Life Sciences","url":"https://www.academia.edu/Documents/in/Life_Sciences?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="32276" href="https://www.academia.edu/Documents/in/Soft_Tissue_Preservation">Soft Tissue Preservation</a>, <script data-card-contents-for-ri="32276" type="text/json">{"id":32276,"name":"Soft Tissue Preservation","url":"https://www.academia.edu/Documents/in/Soft_Tissue_Preservation?f_ri=19295","nofollow":false}</script><a 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cryopreservation","created_at":"2021-07-23T00:12:54.691-07:00","url":"https://www.academia.edu/50181304/Curcumin_treatment_enhances_islet_recovery_by_induction_of_heat_shock_response_proteins_Hsp70_and_heme_oxygenase_1_during_cryopreservation?f_ri=19295","dom_id":"work_50181304","summary":null,"downloadable_attachments":[{"id":68263420,"asset_id":50181304,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35020230,"first_name":"Ramesh","last_name":"Bhonde","domain_name":"mjdrdypu","page_name":"RameshBhonde","display_name":"Ramesh R Bhonde","profile_url":"https://mjdrdypu.academia.edu/RameshBhonde?f_ri=19295","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8014,"name":"Life Sciences","url":"https://www.academia.edu/Documents/in/Life_Sciences?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":32276,"name":"Soft Tissue Preservation","url":"https://www.academia.edu/Documents/in/Soft_Tissue_Preservation?f_ri=19295","nofollow":false},{"id":53663,"name":"HSP","url":"https://www.academia.edu/Documents/in/HSP?f_ri=19295","nofollow":false},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin?f_ri=19295"},{"id":71426,"name":"Insulin Secretion","url":"https://www.academia.edu/Documents/in/Insulin_Secretion?f_ri=19295"},{"id":82978,"name":"Reactive Oxygen Species","url":"https://www.academia.edu/Documents/in/Reactive_Oxygen_Species?f_ri=19295"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=19295"},{"id":93232,"name":"Curcumin","url":"https://www.academia.edu/Documents/in/Curcumin?f_ri=19295"},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=19295"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=19295"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=19295"},{"id":169489,"name":"Heme Oxygenase","url":"https://www.academia.edu/Documents/in/Heme_Oxygenase?f_ri=19295"},{"id":945101,"name":"Liquid Nitrogen","url":"https://www.academia.edu/Documents/in/Liquid_Nitrogen?f_ri=19295"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival?f_ri=19295"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=19295"},{"id":1705044,"name":"islets of Langerhans","url":"https://www.academia.edu/Documents/in/islets_of_Langerhans?f_ri=19295"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_43131344" data-work_id="43131344" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/43131344/Stability_of_Interleukin_6_Soluble_Interleukin_6_Receptor_Interleukin_10_and_CC16_in_Human_Serum">Stability of Interleukin 6, Soluble Interleukin 6 Receptor, Interleukin 10 and CC16 in Human Serum</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/43131344" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="955ff024724b922bb9994c84f57fbed3" rel="nofollow" data-download="{"attachment_id":63389729,"asset_id":43131344,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/63389729/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i 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u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a><script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=43131344]'), work: {"id":43131344,"title":"Stability of Interleukin 6, Soluble Interleukin 6 Receptor, Interleukin 10 and CC16 in Human Serum","created_at":"2020-05-21T19:17:36.385-07:00","url":"https://www.academia.edu/43131344/Stability_of_Interleukin_6_Soluble_Interleukin_6_Receptor_Interleukin_10_and_CC16_in_Human_Serum?f_ri=19295","dom_id":"work_43131344","summary":null,"downloadable_attachments":[{"id":63389729,"asset_id":43131344,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5836108,"first_name":"Michael","last_name":"Maes","domain_name":"independent","page_name":"MichaelMaes","display_name":"Michael Maes","profile_url":"https://independent.academia.edu/MichaelMaes?f_ri=19295","photo":"https://0.academia-photos.com/5836108/2505255/2908910/s65_michael.maes.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=19295","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=19295","nofollow":false},{"id":103298,"name":"Blood sampling","url":"https://www.academia.edu/Documents/in/Blood_sampling?f_ri=19295"},{"id":132592,"name":"Time Delay","url":"https://www.academia.edu/Documents/in/Time_Delay?f_ri=19295"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=19295"},{"id":189424,"name":"Blood Coagulation","url":"https://www.academia.edu/Documents/in/Blood_Coagulation?f_ri=19295"},{"id":295233,"name":"Cytokine","url":"https://www.academia.edu/Documents/in/Cytokine?f_ri=19295"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=19295"},{"id":563659,"name":"Human Serum","url":"https://www.academia.edu/Documents/in/Human_Serum?f_ri=19295"},{"id":1029221,"name":"Freezing","url":"https://www.academia.edu/Documents/in/Freezing?f_ri=19295"},{"id":1186541,"name":"Protein Denaturation","url":"https://www.academia.edu/Documents/in/Protein_Denaturation?f_ri=19295"},{"id":1924712,"name":"Interleukin","url":"https://www.academia.edu/Documents/in/Interleukin?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_38031782" data-work_id="38031782" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/38031782/Effect_of_glutathione_on_sperm_quality_after_short_term_cryopreservation_in_seurukan_fish_Osteochilus_vittatus_Cyprinidae">Effect of glutathione on sperm quality after short-term cryopreservation in seurukan fish Osteochilus vittatus (Cyprinidae</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_38031782" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50 mg L À1. Next, the sperm thawed at 39e40 C for 3 min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6 h after fertilization, while the hatching rate was examined 60 h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteo-chilus vittatus eggs (P < 0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30 mgL À1 of glutathione (63.00 ± 5.89), however, this value was not significantly different from using concentration of 10 and 20 mg L À1. A higher fertilization rate was produced using glutathione concentration of 50 mg L À1 (51.33 ± 17.01); however, it was also not significantly different from the concentration of 20, 30 and 40 mgL À1. In addition, a higher hatching rate was also recorded using glutathione concentration of 50 mg L À1 (40.33 ± 12.89), this value was not significantly different from using 40 mgL À1 glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40 mg L À1 of diluent.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/38031782" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="29622021b69b9e2faa41c40d0f03bac8" rel="nofollow" data-download="{"attachment_id":58054192,"asset_id":38031782,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/58054192/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="197964" href="https://unsyiah.academia.edu/muchlisinza">Zainal A . Muchlisin</a><script data-card-contents-for-user="197964" type="text/json">{"id":197964,"first_name":"Zainal","last_name":"Muchlisin","domain_name":"unsyiah","page_name":"muchlisinza","display_name":"Zainal A . Muchlisin","profile_url":"https://unsyiah.academia.edu/muchlisinza?f_ri=19295","photo":"https://0.academia-photos.com/197964/46993/64243760/s65_zainal.muchlisin.jpeg"}</script></span></span></li><li class="js-paper-rank-work_38031782 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="38031782"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 38031782, container: ".js-paper-rank-work_38031782", }); });</script></li><li class="js-percentile-work_38031782 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 38031782; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_38031782"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_38031782 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="38031782"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 38031782; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=38031782]").text(description); $(".js-view-count-work_38031782").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_38031782").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="38031782"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="151" href="https://www.academia.edu/Documents/in/Cryobiology">Cryobiology</a>, <script data-card-contents-for-ri="151" type="text/json">{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1177" href="https://www.academia.edu/Documents/in/Crystallography">Crystallography</a>, <script data-card-contents-for-ri="1177" type="text/json">{"id":1177,"name":"Crystallography","url":"https://www.academia.edu/Documents/in/Crystallography?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4711" href="https://www.academia.edu/Documents/in/Fisheries">Fisheries</a>, <script data-card-contents-for-ri="4711" type="text/json">{"id":4711,"name":"Fisheries","url":"https://www.academia.edu/Documents/in/Fisheries?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="17375" href="https://www.academia.edu/Documents/in/Fish_Reproduction">Fish Reproduction</a><script data-card-contents-for-ri="17375" type="text/json">{"id":17375,"name":"Fish Reproduction","url":"https://www.academia.edu/Documents/in/Fish_Reproduction?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=38031782]'), work: {"id":38031782,"title":"Effect of glutathione on sperm quality after short-term cryopreservation in seurukan fish Osteochilus vittatus (Cyprinidae","created_at":"2018-12-23T23:35:27.588-08:00","url":"https://www.academia.edu/38031782/Effect_of_glutathione_on_sperm_quality_after_short_term_cryopreservation_in_seurukan_fish_Osteochilus_vittatus_Cyprinidae?f_ri=19295","dom_id":"work_38031782","summary":"The objective of this present study is to determine the optimum concentration for glutathione in the cryopreservation of seurukan fish (Osteochilus vittatus) spermatozoa. A completely randomized design (CRD) was used with 6 treatments and 3 replications. The Seurukan fish sperm was diluted in extender with a ratio of 1:20 (sperm: extender), then glutathione was added at a concentration of 0, 10, 20, 30, 40 and 50 mg L À1. Next, the sperm thawed at 39e40 C for 3 min and mixed with 100 eggs which were randomly selected. The success of the fertilized egg was observed 6 h after fertilization, while the hatching rate was examined 60 h after fertilization. The ANOVA test showed that the addition of glutathione significant affected the sperms motility, fertility and hatching rate of seurukan fish Osteo-chilus vittatus eggs (P \u003c 0.05). By segregation the fresh sperm, a higher sperm motility rate was recorded with an addition of 30 mgL À1 of glutathione (63.00 ± 5.89), however, this value was not significantly different from using concentration of 10 and 20 mg L À1. A higher fertilization rate was produced using glutathione concentration of 50 mg L À1 (51.33 ± 17.01); however, it was also not significantly different from the concentration of 20, 30 and 40 mgL À1. In addition, a higher hatching rate was also recorded using glutathione concentration of 50 mg L À1 (40.33 ± 12.89), this value was not significantly different from using 40 mgL À1 glutathione. Hence, a conclusion was drawn that the optimum concentration of glutathione is 40 mg L À1 of diluent.","downloadable_attachments":[{"id":58054192,"asset_id":38031782,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":197964,"first_name":"Zainal","last_name":"Muchlisin","domain_name":"unsyiah","page_name":"muchlisinza","display_name":"Zainal A . Muchlisin","profile_url":"https://unsyiah.academia.edu/muchlisinza?f_ri=19295","photo":"https://0.academia-photos.com/197964/46993/64243760/s65_zainal.muchlisin.jpeg"}],"research_interests":[{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false},{"id":1177,"name":"Crystallography","url":"https://www.academia.edu/Documents/in/Crystallography?f_ri=19295","nofollow":false},{"id":4711,"name":"Fisheries","url":"https://www.academia.edu/Documents/in/Fisheries?f_ri=19295","nofollow":false},{"id":17375,"name":"Fish Reproduction","url":"https://www.academia.edu/Documents/in/Fish_Reproduction?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295"},{"id":23848,"name":"Aquaculture","url":"https://www.academia.edu/Documents/in/Aquaculture?f_ri=19295"},{"id":36977,"name":"Fish Breeding","url":"https://www.academia.edu/Documents/in/Fish_Breeding?f_ri=19295"},{"id":342400,"name":"Spermatozoa Cryobiology","url":"https://www.academia.edu/Documents/in/Spermatozoa_Cryobiology?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_7572250" data-work_id="7572250" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/7572250/Improved_vitrification_solutions_based_on_the_predictability_of_vitrification_solution_toxicity">Improved vitrification solutions based on the predictability of vitrification solution toxicity</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a 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toxicity","created_at":"2014-07-06T04:51:41.717-07:00","url":"https://www.academia.edu/7572250/Improved_vitrification_solutions_based_on_the_predictability_of_vitrification_solution_toxicity?f_ri=19295","dom_id":"work_7572250","summary":null,"downloadable_attachments":[{"id":48417955,"asset_id":7572250,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4088138,"first_name":"Gregory","last_name":"Fahy","domain_name":"independent","page_name":"GregoryFahy","display_name":"Gregory Fahy","profile_url":"https://independent.academia.edu/GregoryFahy?f_ri=19295","photo":"https://0.academia-photos.com/4088138/1573856/1905987/s65_gregory.fahy.jpg"}],"research_interests":[{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false},{"id":2699,"name":"Tissue Engineering","url":"https://www.academia.edu/Documents/in/Tissue_Engineering?f_ri=19295","nofollow":false},{"id":9003,"name":"Kidney transplantation","url":"https://www.academia.edu/Documents/in/Kidney_transplantation?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":32276,"name":"Soft Tissue Preservation","url":"https://www.academia.edu/Documents/in/Soft_Tissue_Preservation?f_ri=19295"},{"id":71294,"name":"Kidney","url":"https://www.academia.edu/Documents/in/Kidney?f_ri=19295"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=19295"},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=19295"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=19295"},{"id":147636,"name":"Cooling Rate","url":"https://www.academia.edu/Documents/in/Cooling_Rate?f_ri=19295"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=19295"},{"id":203424,"name":"Organ Preservation","url":"https://www.academia.edu/Documents/in/Organ_Preservation?f_ri=19295"},{"id":240148,"name":"Hydrogen Bond","url":"https://www.academia.edu/Documents/in/Hydrogen_Bond?f_ri=19295"},{"id":335228,"name":"Glass Transition Temperature","url":"https://www.academia.edu/Documents/in/Glass_Transition_Temperature?f_ri=19295"},{"id":351461,"name":"Biological systems","url":"https://www.academia.edu/Documents/in/Biological_systems?f_ri=19295"},{"id":389153,"name":"Polyvinyl alcohol","url":"https://www.academia.edu/Documents/in/Polyvinyl_alcohol?f_ri=19295"},{"id":414692,"name":"Solutions","url":"https://www.academia.edu/Documents/in/Solutions?f_ri=19295"},{"id":613442,"name":"Fertilization in Vitro","url":"https://www.academia.edu/Documents/in/Fertilization_in_Vitro?f_ri=19295"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits?f_ri=19295"},{"id":1438730,"name":"Creatinine","url":"https://www.academia.edu/Documents/in/Creatinine?f_ri=19295"},{"id":1650162,"name":"Ethylene Glycol","url":"https://www.academia.edu/Documents/in/Ethylene_Glycol?f_ri=19295"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9522492" data-work_id="9522492" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9522492/DNA_fragmentation_in_frozen_sperm_of_Equus_asinus_Zamorano_Leon%C3%A9s_a_breed_at_risk_of_extinction">DNA fragmentation in frozen sperm of Equus asinus: Zamorano-Leonés, a breed at risk of extinction</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9522492" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2d044034ecc18d08a37dcffb0181c808" rel="nofollow" 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href="https://www.academia.edu/21651338/Mechanics_of_Fresh_Refrigerated_and_Frozen_Arterial_Tissue">Mechanics of Fresh, Refrigerated, and Frozen Arterial Tissue</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21651338" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b960c019e377b6770b2ad8a796653202" rel="nofollow" data-download="{"attachment_id":42292450,"asset_id":21651338,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen 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Freezing Tetrahymena thermophila</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We have developed a simple, efficient procedure for the long term freezing of Tetrahymena thermophila in liquid nitrogen. This technique yields excellent recovery of viable cells with all strains tested and does not require the use of a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18944158" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We have developed a simple, efficient procedure for the long term freezing of Tetrahymena thermophila in liquid nitrogen. This technique yields excellent recovery of viable cells with all strains tested and does not require the use of a controlled rate low temperature freezer. To optimize the freezing technique, we have examined the effects of varying a number of parameters, including the physiological state of the cells prior to freezing, the time of exposure to cryoprotectant, and the rate of freezing and thawing. The frequency of viable cell recovery following freezing using this technique has been tested for a variety of different cell lines.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18944158" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8ed3d567b67fd7293b3799e88743d560" rel="nofollow" data-download="{"attachment_id":42125614,"asset_id":18944158,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42125614/download_file?st=MTczMjQ0MDY4Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39077383" href="https://independent.academia.edu/DCassidyhanley">D. 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This technique yields excellent recovery of viable cells with all strains tested and does not require the use of a controlled rate low temperature freezer. To optimize the freezing technique, we have examined the effects of varying a number of parameters, including the physiological state of the cells prior to freezing, the time of exposure to cryoprotectant, and the rate of freezing and thawing. The frequency of viable cell recovery following freezing using this technique has been tested for a variety of different cell lines.","downloadable_attachments":[{"id":42125614,"asset_id":18944158,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39077383,"first_name":"D.","last_name":"Cassidy-hanley","domain_name":"independent","page_name":"DCassidyhanley","display_name":"D. 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However, ER+/PR− tumors respond less well. To... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9774993" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The presence of progesterone receptor (PR) in estrogen receptor (ER)-positive breast cancer is associated with a good prognosis, and indicates that tumors are likely to respond to tamoxifen. However, ER+/PR− tumors respond less well. To reveal the potential molecular mechanism of this phenomenon, we sought to identify differential protein abundances between invasive ductal carcinoma cells from cryopreserved ER+/PR+ and ER+/PR− mammary tumor specimens. Because current proteomics methods are hampered in the examination of most primary human tumor samples by the extreme tissue heterogeneity, we used laser capture microdissection (LCM) to isolate tumor cells and developed a sample pooling strategy to analyze small sample protein lysates. Proteins from LCM-harvested tumors were pooled into four sub-pools from each condition of three tumors/sub-pool, and proteins from respective paired sub-pools were co-electrophoresed by 2-DE using 54-cm IEF over pH 4–9. Abundance ratios were accurately quantified by a differential multiplex radioactive ProteoTope method at low attomole levels (∼3.6 &&mu;;g protein per labeling reaction, <180 ng per multiplex protein sample per 54-cm gel). Applying this approach, differentially displayed proteins were identified by MS using comigrating non-radioactively labeled tumor proteins. They include decreased cytochrome b5 and transgelin, and more abundant CRABP-II, cyclophilin A, Neudesin, and hemoglobin in ER+/PR+ tumors versus ER+/PR−providing a possible explanation for differential susceptibility against tamoxifen as a result of deregulated cytochrome b5-dependent metabolism. This study demonstrates the potential of ProteoTope and LCM to enable extremely sensitive and precise differential analyses from well-defined primary clinical specimen.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9774993" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cb492776adea087c2c523e21eb983647" rel="nofollow" data-download="{"attachment_id":47651345,"asset_id":9774993,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47651345/download_file?st=MTczMjQ0MDY4NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="23539420" href="https://eitplus.academia.edu/WojciechWozny">Wojciech Wozny</a><script data-card-contents-for-user="23539420" type="text/json">{"id":23539420,"first_name":"Wojciech","last_name":"Wozny","domain_name":"eitplus","page_name":"WojciechWozny","display_name":"Wojciech Wozny","profile_url":"https://eitplus.academia.edu/WojciechWozny?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_9774993 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9774993"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9774993, container: ".js-paper-rank-work_9774993", }); 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However, ER+/PR− tumors respond less well. To reveal the potential molecular mechanism of this phenomenon, we sought to identify differential protein abundances between invasive ductal carcinoma cells from cryopreserved ER+/PR+ and ER+/PR− mammary tumor specimens. Because current proteomics methods are hampered in the examination of most primary human tumor samples by the extreme tissue heterogeneity, we used laser capture microdissection (LCM) to isolate tumor cells and developed a sample pooling strategy to analyze small sample protein lysates. Proteins from LCM-harvested tumors were pooled into four sub-pools from each condition of three tumors/sub-pool, and proteins from respective paired sub-pools were co-electrophoresed by 2-DE using 54-cm IEF over pH 4–9. Abundance ratios were accurately quantified by a differential multiplex radioactive ProteoTope method at low attomole levels (∼3.6 \u0026\u0026mu;;g protein per labeling reaction, \u003c180 ng per multiplex protein sample per 54-cm gel). Applying this approach, differentially displayed proteins were identified by MS using comigrating non-radioactively labeled tumor proteins. They include decreased cytochrome b5 and transgelin, and more abundant CRABP-II, cyclophilin A, Neudesin, and hemoglobin in ER+/PR+ tumors versus ER+/PR−providing a possible explanation for differential susceptibility against tamoxifen as a result of deregulated cytochrome b5-dependent metabolism. 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href="https://www.academia.edu/59046241/Phase_contrast_x_ray_imaging_and_tomography_of_the_nematode_Caenorhabditis_elegans">Phase-contrast x-ray imaging and tomography of the nematode Caenorhabditis elegans</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/59046241" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7793bb0ddcae1b29c381ff0ba09bef46" rel="nofollow" data-download="{"attachment_id":73165080,"asset_id":59046241,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" 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data-has-card-for-ri="2184" href="https://www.academia.edu/Documents/in/Electron_Microscopy">Electron Microscopy</a>, <script data-card-contents-for-ri="2184" type="text/json">{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2889" href="https://www.academia.edu/Documents/in/Caenorhabditis_elegans">Caenorhabditis elegans</a><script data-card-contents-for-ri="2889" type="text/json">{"id":2889,"name":"Caenorhabditis elegans","url":"https://www.academia.edu/Documents/in/Caenorhabditis_elegans?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=59046241]'), work: {"id":59046241,"title":"Phase-contrast x-ray imaging and tomography of the nematode Caenorhabditis 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href="https://www.academia.edu/25764841/Novel_application_of_a_whole_blood_intracellular_cytokine_detection_assay_to_quantitate_specific_T_cell_frequency_in_field_studies">Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25764841" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="97f76f1d5c821f85dc7d2944333de49b" rel="nofollow" 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u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of genotoxicity tests in germ cells is to assess the impact of exposure to environmental mutagens that may represent a risk for the fertility or for the offspring of exposed subject. The comet assay on mature mouse oocytes is a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23837494" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of genotoxicity tests in germ cells is to assess the impact of exposure to environmental mutagens that may represent a risk for the fertility or for the offspring of exposed subject. The comet assay on mature mouse oocytes is a simple, reproductive and rapid test to study primary DNA damage in oocytes. This test is used to complete toxicology assays applied in first line to somatic cells, and could find many applications in reproductive toxicology to study impact of environmental factors on female germ cells. We describe a practical application of comet assay in reproductive biology to assess the genotoxicity of cryoprotectants used at high concentrations in oocyte vitrification protocols. This test allowed us to demonstrate that dimethylsulfoxide and ethylene glycol are non-genotoxic for the mouse oocytes and led us to hypothesize a genotoxic effect of 1,2-propanediol (PrOH) at high concentrations after having observed induction of significant DNA damage on CHO cell line an...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23837494" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="86530f9718fee51543182c315ad6095b" rel="nofollow" data-download="{"attachment_id":44237957,"asset_id":23837494,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44237957/download_file?st=MTczMjQ0MDY4NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46082625" href="https://independent.academia.edu/JeannePerrin1">Jeanne Perrin</a><script data-card-contents-for-user="46082625" type="text/json">{"id":46082625,"first_name":"Jeanne","last_name":"Perrin","domain_name":"independent","page_name":"JeannePerrin1","display_name":"Jeanne Perrin","profile_url":"https://independent.academia.edu/JeannePerrin1?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23837494 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23837494"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23837494, container: ".js-paper-rank-work_23837494", }); 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href="https://www.academia.edu/16526199/Results_of_6139_artificial_insemination_cycles_with_donor_spermatozoa">Results of 6139 artificial insemination cycles with donor spermatozoa</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16526199" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="66c158eaf9a2a575163e63a6a46907cf" rel="nofollow" data-download="{"attachment_id":42448005,"asset_id":16526199,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm 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It became apparent that these materials could be successfully reused as new techniques and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13703977" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">By the early 1980s, many life scientists had begun to maintain small collections of cryopreserved<br />tissues for their own specific research purposes. It became apparent that these materials could be<br />successfully reused as new techniques and research questions emerged. This realization led several<br />American leaders in the field of systematic taxonomy (the science of biological classification) and<br />conservation genetics to argue for the need to take stock of and coordinate these heterogeneous<br />collections. Their strategy, which they called ‘planned hindsight,’ was meant to organize the<br />present in a way that appeared to anticipate the needs of future scientists. In this paper I examine<br />how the seemingly paradoxical strategy of ‘planned hindsight’ has functioned as a strategy for<br />choreographing life, time, and value at two centralized biospecimen collections: The Frozen Zoo in<br />Escondido, CA, USA, and the Ambrose Monell Cryo Collection at the American Museum of Natural<br />History in New York City. I conclude that, in practice, ‘planned hindsight’ not only contributes to the<br />endurance of frozen tissues but also preserves widely divergent speculative visions of the many<br />different individuals involved with their creation, maintenance, and re-use.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13703977" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="be6f51ff45df0b3789da122b91dadf22" rel="nofollow" data-download="{"attachment_id":39557836,"asset_id":13703977,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39557836/download_file?st=MTczMjQ0MDY4NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="96670" href="https://yale.academia.edu/JoannaRadin">Joanna Radin</a><script data-card-contents-for-user="96670" type="text/json">{"id":96670,"first_name":"Joanna","last_name":"Radin","domain_name":"yale","page_name":"JoannaRadin","display_name":"Joanna Radin","profile_url":"https://yale.academia.edu/JoannaRadin?f_ri=19295","photo":"https://0.academia-photos.com/96670/26730/7189493/s65_joanna.radin.jpg"}</script></span></span></li><li class="js-paper-rank-work_13703977 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13703977"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13703977, container: ".js-paper-rank-work_13703977", }); 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It became apparent that these materials could be\nsuccessfully reused as new techniques and research questions emerged. This realization led several\nAmerican leaders in the field of systematic taxonomy (the science of biological classification) and\nconservation genetics to argue for the need to take stock of and coordinate these heterogeneous\ncollections. Their strategy, which they called ‘planned hindsight,’ was meant to organize the\npresent in a way that appeared to anticipate the needs of future scientists. In this paper I examine\nhow the seemingly paradoxical strategy of ‘planned hindsight’ has functioned as a strategy for\nchoreographing life, time, and value at two centralized biospecimen collections: The Frozen Zoo in\nEscondido, CA, USA, and the Ambrose Monell Cryo Collection at the American Museum of Natural\nHistory in New York City. I conclude that, in practice, ‘planned hindsight’ not only contributes to the\nendurance of frozen tissues but also preserves widely divergent speculative visions of the many\ndifferent individuals involved with their creation, maintenance, and re-use.","downloadable_attachments":[{"id":39557836,"asset_id":13703977,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":96670,"first_name":"Joanna","last_name":"Radin","domain_name":"yale","page_name":"JoannaRadin","display_name":"Joanna Radin","profile_url":"https://yale.academia.edu/JoannaRadin?f_ri=19295","photo":"https://0.academia-photos.com/96670/26730/7189493/s65_joanna.radin.jpg"}],"research_interests":[{"id":2988,"name":"History of Natural History","url":"https://www.academia.edu/Documents/in/History_of_Natural_History?f_ri=19295","nofollow":false},{"id":9201,"name":"Anthropology Of Science (Science Technology And 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class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/78107916/Sea_urchin_Paracentrotus_lividus_larval_rearing_Culture_from_cryopreserved_embryos">Sea urchin (Paracentrotus lividus) larval rearing — Culture from cryopreserved embryos</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/78107916" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="03e60033d1ab91256e730d11b25b23d0" rel="nofollow" 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embryos","url":"https://www.academia.edu/Documents/in/Cryopreservation_of_gametes_and_embryos?f_ri=19295"},{"id":170652,"name":"Fisheries Sciences","url":"https://www.academia.edu/Documents/in/Fisheries_Sciences?f_ri=19295"},{"id":229847,"name":"Sea Urchins","url":"https://www.academia.edu/Documents/in/Sea_Urchins?f_ri=19295"},{"id":560354,"name":"Sea Urchin","url":"https://www.academia.edu/Documents/in/Sea_Urchin?f_ri=19295"},{"id":771480,"name":"Paracentrotus lividus","url":"https://www.academia.edu/Documents/in/Paracentrotus_lividus?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_49502890" data-work_id="49502890" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/49502890/Recovery_and_cryopreservation_of_epididymal_sperm_from_agouti_Dasiprocta_aguti_using_powdered_coconut_water_ACP_109c_and_Tris_extenders">Recovery and cryopreservation of epididymal sperm from agouti (Dasiprocta aguti) using powdered coconut water (ACP-109c) and Tris extenders</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/49502890" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="da0dd86b427521f0bdc9aed9dee291e3" rel="nofollow" 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Analysis","url":"https://www.academia.edu/Documents/in/Semen_Analysis?f_ri=19295"},{"id":143078,"name":"Cocos","url":"https://www.academia.edu/Documents/in/Cocos?f_ri=19295"},{"id":541736,"name":"Sexual maturity","url":"https://www.academia.edu/Documents/in/Sexual_maturity?f_ri=19295"},{"id":559224,"name":"Membrane Integrity","url":"https://www.academia.edu/Documents/in/Membrane_Integrity?f_ri=19295"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=19295"},{"id":897074,"name":"Sperm Retrieval","url":"https://www.academia.edu/Documents/in/Sperm_Retrieval?f_ri=19295"},{"id":897075,"name":"Epididymis","url":"https://www.academia.edu/Documents/in/Epididymis?f_ri=19295"},{"id":945101,"name":"Liquid Nitrogen","url":"https://www.academia.edu/Documents/in/Liquid_Nitrogen?f_ri=19295"},{"id":1223743,"name":"Sperm Quality","url":"https://www.academia.edu/Documents/in/Sperm_Quality?f_ri=19295"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=19295"},{"id":3998792,"name":"Egg yolk","url":"https://www.academia.edu/Documents/in/Egg_yolk?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_26365785" data-work_id="26365785" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/26365785/Cryopreservation_of_cultivated_and_wild_Arachis_species_embryonic_axes_using_desiccation_and_vitrification_methods">Cryopreservation of cultivated and wild Arachis species embryonic axes using desiccation and vitrification methods</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_26365785" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/26365785" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a6bba84e7ddcb3c16416b87c71de185e" rel="nofollow" data-download="{"attachment_id":46672190,"asset_id":26365785,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46672190/download_file?st=MTczMjQ0MDY4NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50233802" href="https://uerj.academia.edu/RachelGagliardi">Rachel Gagliardi</a><script data-card-contents-for-user="50233802" type="text/json">{"id":50233802,"first_name":"Rachel","last_name":"Gagliardi","domain_name":"uerj","page_name":"RachelGagliardi","display_name":"Rachel Gagliardi","profile_url":"https://uerj.academia.edu/RachelGagliardi?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_26365785 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="26365785"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 26365785, container: ".js-paper-rank-work_26365785", }); 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$(".js-view-count[data-work-id=26365785]").text(description); $(".js-view-count-work_26365785").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_26365785").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="26365785"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a>, <script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="151091" href="https://www.academia.edu/Documents/in/Nitrogen">Nitrogen</a>, <script data-card-contents-for-ri="151091" type="text/json">{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="162196" href="https://www.academia.edu/Documents/in/Sucrose">Sucrose</a>, <script data-card-contents-for-ri="162196" type="text/json">{"id":162196,"name":"Sucrose","url":"https://www.academia.edu/Documents/in/Sucrose?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="165000" href="https://www.academia.edu/Documents/in/Desiccation">Desiccation</a><script data-card-contents-for-ri="165000" type="text/json">{"id":165000,"name":"Desiccation","url":"https://www.academia.edu/Documents/in/Desiccation?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=26365785]'), work: {"id":26365785,"title":"Cryopreservation of cultivated and wild Arachis species embryonic axes using desiccation and vitrification methods","created_at":"2016-06-21T04:48:45.989-07:00","url":"https://www.academia.edu/26365785/Cryopreservation_of_cultivated_and_wild_Arachis_species_embryonic_axes_using_desiccation_and_vitrification_methods?f_ri=19295","dom_id":"work_26365785","summary":"The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment.","downloadable_attachments":[{"id":46672190,"asset_id":26365785,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50233802,"first_name":"Rachel","last_name":"Gagliardi","domain_name":"uerj","page_name":"RachelGagliardi","display_name":"Rachel Gagliardi","profile_url":"https://uerj.academia.edu/RachelGagliardi?f_ri=19295","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=19295","nofollow":false},{"id":162196,"name":"Sucrose","url":"https://www.academia.edu/Documents/in/Sucrose?f_ri=19295","nofollow":false},{"id":165000,"name":"Desiccation","url":"https://www.academia.edu/Documents/in/Desiccation?f_ri=19295","nofollow":false},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=19295"},{"id":283379,"name":"Glycerol","url":"https://www.academia.edu/Documents/in/Glycerol?f_ri=19295"},{"id":386438,"name":"Seeds","url":"https://www.academia.edu/Documents/in/Seeds?f_ri=19295"},{"id":1146416,"name":"Arachis Hypogaea","url":"https://www.academia.edu/Documents/in/Arachis_Hypogaea?f_ri=19295"},{"id":2474622,"name":"Recovery of Function","url":"https://www.academia.edu/Documents/in/Recovery_of_Function?f_ri=19295"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17965233" data-work_id="17965233" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17965233/T_cell_responses_in_fresh_and_cryopreserved_peripheral_blood_mononuclear_cells_Kinetics_of_cell_viability_cellular_subsets_proliferation_and_cytokine_production">T cell responses in fresh and cryopreserved peripheral blood mononuclear cells: Kinetics of cell viability, cellular subsets, proliferation, and cytokine production</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Polyclonal stimuli like phorbol myristate acetate (PMA) plus calcium ionophore (Ca-I), concanavalin A (ConA) or anti-CD3 plus anti-CD28 (alphaCD3/alphaCD28) are widely used T cell stimuli. All three stimuli act at different sites and in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_17965233" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Polyclonal stimuli like phorbol myristate acetate (PMA) plus calcium ionophore (Ca-I), concanavalin A (ConA) or anti-CD3 plus anti-CD28 (alphaCD3/alphaCD28) are widely used T cell stimuli. All three stimuli act at different sites and in different ways to activate the T cell receptor pathway and are widely used in different concentrations, stimulation durations and read-out systems. This study was designed to establish the most suitable polyclonal stimulus in human peripheral blood mononuclear cells (PBMC) experiments by assessing the kinetics of cell viability, present immunophenotypes, proliferation, and cytokine production of the PBMC. In addition, changes in these read-out parameters due to cryopreservation have been investigated by comparing fresh and cryopreserved PBMC cultures at days 1, 3, 5, and 7. This study showed a reduction in the cytokine levels after cryopreservation of PMA/Ca-I stimulated PBMC, whereas no significant differences due to the cryopreservation were observed in ConA or alphaCD3/alphaCD28 stimulated PBMC. Cryopreservation did not alter the maximal proliferation capacity of ConA or alphaCD3/alphaCD28 stimulated PBMC, whereas it did delay the proliferation. Although cryopreservation had no effect on the CD3+CD4+ or CD3+CD8+ T cell subsets, PMA/Ca-I significantly reduced the amount of both T cell subsets over time. In conclusion, PMA/Ca-I is suitable as a positive control in experiments where high cytokine production is expected and only fresh PBMC are used. Proliferation and effects on the T cell subsets in long-term PBMC cultures should use ConA or alphaCD3/alphaCD28 as positive control.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17965233" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e5320d20ba949bcb59c5e906058d3052" rel="nofollow" data-download="{"attachment_id":42213934,"asset_id":17965233,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42213934/download_file?st=MTczMjQ0MDY4NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37888813" href="https://independent.academia.edu/PJeurink">Prescilla Jeurink</a><script data-card-contents-for-user="37888813" type="text/json">{"id":37888813,"first_name":"Prescilla","last_name":"Jeurink","domain_name":"independent","page_name":"PJeurink","display_name":"Prescilla Jeurink","profile_url":"https://independent.academia.edu/PJeurink?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_17965233 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="17965233"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 17965233, container: ".js-paper-rank-work_17965233", }); 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$(".js-view-count[data-work-id=17965233]").text(description); $(".js-view-count-work_17965233").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_17965233").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="17965233"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="151" href="https://www.academia.edu/Documents/in/Cryobiology">Cryobiology</a>, <script data-card-contents-for-ri="151" type="text/json">{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9111" href="https://www.academia.edu/Documents/in/Cytokines">Cytokines</a>, <script data-card-contents-for-ri="9111" type="text/json">{"id":9111,"name":"Cytokines","url":"https://www.academia.edu/Documents/in/Cytokines?f_ri=19295","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19295" href="https://www.academia.edu/Documents/in/Cryopreservation">Cryopreservation</a><script data-card-contents-for-ri="19295" type="text/json">{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17965233]'), work: {"id":17965233,"title":"T cell responses in fresh and cryopreserved peripheral blood mononuclear cells: Kinetics of cell viability, cellular subsets, proliferation, and cytokine production","created_at":"2015-11-08T10:51:55.324-08:00","url":"https://www.academia.edu/17965233/T_cell_responses_in_fresh_and_cryopreserved_peripheral_blood_mononuclear_cells_Kinetics_of_cell_viability_cellular_subsets_proliferation_and_cytokine_production?f_ri=19295","dom_id":"work_17965233","summary":"Polyclonal stimuli like phorbol myristate acetate (PMA) plus calcium ionophore (Ca-I), concanavalin A (ConA) or anti-CD3 plus anti-CD28 (alphaCD3/alphaCD28) are widely used T cell stimuli. All three stimuli act at different sites and in different ways to activate the T cell receptor pathway and are widely used in different concentrations, stimulation durations and read-out systems. This study was designed to establish the most suitable polyclonal stimulus in human peripheral blood mononuclear cells (PBMC) experiments by assessing the kinetics of cell viability, present immunophenotypes, proliferation, and cytokine production of the PBMC. In addition, changes in these read-out parameters due to cryopreservation have been investigated by comparing fresh and cryopreserved PBMC cultures at days 1, 3, 5, and 7. This study showed a reduction in the cytokine levels after cryopreservation of PMA/Ca-I stimulated PBMC, whereas no significant differences due to the cryopreservation were observed in ConA or alphaCD3/alphaCD28 stimulated PBMC. Cryopreservation did not alter the maximal proliferation capacity of ConA or alphaCD3/alphaCD28 stimulated PBMC, whereas it did delay the proliferation. Although cryopreservation had no effect on the CD3+CD4+ or CD3+CD8+ T cell subsets, PMA/Ca-I significantly reduced the amount of both T cell subsets over time. In conclusion, PMA/Ca-I is suitable as a positive control in experiments where high cytokine production is expected and only fresh PBMC are used. Proliferation and effects on the T cell subsets in long-term PBMC cultures should use ConA or alphaCD3/alphaCD28 as positive control.","downloadable_attachments":[{"id":42213934,"asset_id":17965233,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37888813,"first_name":"Prescilla","last_name":"Jeurink","domain_name":"independent","page_name":"PJeurink","display_name":"Prescilla Jeurink","profile_url":"https://independent.academia.edu/PJeurink?f_ri=19295","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":151,"name":"Cryobiology","url":"https://www.academia.edu/Documents/in/Cryobiology?f_ri=19295","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=19295","nofollow":false},{"id":9111,"name":"Cytokines","url":"https://www.academia.edu/Documents/in/Cytokines?f_ri=19295","nofollow":false},{"id":19295,"name":"Cryopreservation","url":"https://www.academia.edu/Documents/in/Cryopreservation?f_ri=19295","nofollow":false},{"id":34021,"name":"T cell receptor","url":"https://www.academia.edu/Documents/in/T_cell_receptor?f_ri=19295"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=19295"},{"id":70247,"name":"Natural Killer cells","url":"https://www.academia.edu/Documents/in/Natural_Killer_cells?f_ri=19295"},{"id":165002,"name":"Cell 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class="download"><a id="5f30e0de437664ae74517eee221a1a81" rel="nofollow" data-download="{"attachment_id":37795859,"asset_id":12727323,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/37795859/download_file?st=MTczMjQ0MDY4NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33646271" href="https://independent.academia.edu/PeterMazur1">Peter Mazur</a><script data-card-contents-for-user="33646271" type="text/json">{"id":33646271,"first_name":"Peter","last_name":"Mazur","domain_name":"independent","page_name":"PeterMazur1","display_name":"Peter Mazur","profile_url":"https://independent.academia.edu/PeterMazur1?f_ri=19295","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-12727323">+1</span><div class="hidden js-additional-users-12727323"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://uvigo.academia.edu/EstefaniaParedesRosendo">Estefania Paredes Rosendo</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-12727323'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-12727323').html(); } } new 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/56501395/Efficient_method_for_slow_cryopreservation_of_human_embryonic_stem_cells_in_xeno_free_conditions">Efficient method for slow cryopreservation of human embryonic stem cells in xeno-free conditions</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/56501395" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cbdc04d3da9c1305ccbf4eb558fca645" rel="nofollow" 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href="https://www.academia.edu/33644370/Cryopreservation_of_the_ovary_by_vitrification_as_an_alternative_to_slow_cooling_protocols">Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/33644370" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dcb30c7bff4ba25a8f10a46a81cf32da" rel="nofollow" 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