GENEWIZ from Azenta | Standard Plasmid DNA Preparation

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<div> </div> <p>Delivering high quality DNA from a variety of starting materials in a cost-effective and reliable manner is our priority. Don't waste your valuable time with a plasmid preparation kit when GENEWIZ<sup>TM</sup> Plasmid Preparation Services from Azenta can conveniently provide <a href="https://www.genewiz.com/en/Public/Services/Plasmid-DNA-Prep">plasmid DNA preps</a> at the scale and quantity you need.</p> <p>Our standard plasmid DNA preparation service is a flexible and fast choice for a range of plasmid templates. Runs are available in the classic mini to giga scales, yielding micrograms to milligrams of purified plasmid DNA. Additional add-on service and analysis options are available, enabling project customization to meet budgetary and technical specifications.</p> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/DNAPrep?lobId=5&serviceId=510" target="_blank"><button>Place an Order</button></a> </div> <div class="plasmid-content-box" id="plasmid-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/prep25?_ga=2.22954226.858825492.1654525039-1258591845.1617043252" target="_blank"><img alt="" src="/-/media/Images/15005D-Prep20OffPromoWebBanRightV1.ashx?la=en&hash=8698D8122942CE889C52D33DE6808F41725FDDAD" /></a> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> No plasmid purification kit needed – Submit starting material and Azenta will deliver your purified plasmid product </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Fast delivery time - Rapid turnaround time, starting at just two business days, to meet your research deadlines </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Convenient online ordering - Place an order directly through your Azenta account, including your Sanger sequencing results page </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Quality control – Strict QC process with multiple analysis options to ensure high-quality plasmid prep </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url('/-/media/15016-D_Plasmid-EZ_WebBanFull-Med_Background.ashx'); background-size: cover; background-position: center center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 500; line-height: 100%; font-size: 50px; margin: 0; padding-bottom: 15px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h4 style="color: #826ec3;"><strong>NEW</strong></h4> <h3>Plasmid-EZ</h3> <p>Whole plasmid sequencing in as fast as 1 business day upon receipt in processing lab</p> <br /> <a href="https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ" target="_blank"> <br /> <button> LEARN MORE </button> <br /> </a> <p> </p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <p style="text-align: center;"> </p> <style> .table-responsive { overflow-x: hidden} </style> <p> </p> <div class="container margin-top-50 margin-bottom-50" style="text-align: center;"> <h1>Plasmid DNA Prep Service & Analysis Options</h1> <p style="text-align: left;"><em class="BlueCheck icon-4x fa fa-check" style="font-size: 20px; float: none; vertical-align: middle;"></em> = Included</p> <p style="text-align: left;"><em class="BlueCheck icon-4x fa fa-plus" style="font-size: 20px; float: none; vertical-align: middle; color: black;"></em> = Add-on</p> <div class="table-responsive" style="text-align: center;"> <table class="table table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td style="padding:10px;">Turnaround Time</td> <td style="padding:10px;">Accepted Plasmids</td> <td style="padding:10px;">Service Options</td> <td style="padding:10px;">Analysis Options</td> </tr> <tr> <td rowspan="8" style="padding:10px;"> <p>Starting from 2-3 business days</p> </td> <td style="text-align: left; padding:10px;"> <ul style="width: auto; display: inline-block;"> <li style="text-align: left;">High- or Low-Copy</li> <li style="text-align: left;">Toxic Construct</li> <li style="text-align: left;">Stable or Unstable</li> </ul> </td> <td style="text-align: left; padding:10px;"> <span class="BlueCheck icon-4x fa fa-check" style="font-size: 14px; float: none; text-align: left; vertical-align: middle;"><span style="font-size: 14px; text-align: left; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; font-family: 'Open Sans', sans-serif; color: #2f2f2f;"></span><span style="font-size: 14px; text-align: left; font-family: 'Open Sans', sans-serif; color: #585f69;"> Spectrophotometric DNA Quality Analysis</span></span> <br /> <span class="BlueCheck icon-4x fa fa-check" style="font-size: 14px; float: none; text-align: left; vertical-align: middle;"><span style="font-size: 14px; text-align: left; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; font-family: 'Open Sans', sans-serif; color: #2f2f2f;"></span><span style="font-size: 14px; text-align: left; font-family: 'Open Sans', sans-serif; color: #585f69;"> Minimum Yield Verification</span></span> <br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> Transfection-Grade (Endotoxin Free)</span> <br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> DNA Normalization</span> <br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> Aliquots or Custom Tubes</span><br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> GLP-Compliant</span><br /> </td> <td style="text-align: left; padding:10px;"> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> DNA Sequencing</span><br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> Sequence Consensus Assembly & Analysis</span><br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> Full-Service DNA Sequence Confirmation</span><br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> Restriction Digest & Agarose Gel Analysis</span><br /> <span class="BlueCheck icon-4x fa fa-plus" style="font-size: 14px; float: none; text-align: left; vertical-align: middle; color: black;"></span><span style="text-align: left;"></span><span style="font-size: 14px; text-align: center; font-family: 'Open Sans', sans-serif; color: #585f69;"> Sterility Testing</span><br /> </td> </tr> </tbody> </table> <p style="text-align: center;">Not all options available for mini-scale standard and 96-well preps. Please contact <a href="mailto:GS@azenta.com">GS@azenta.com</a> to discuss your miniprep project. </p> <h3>Upgrade to our amount based <a href="/Public/Services/Plasmid-DNA-Prep/Targeted-Yield">Targeted Yield Service</a>. Transfection-grade and value-added analysis options included.</h3> </div> </div> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>DNA PREPARATION SCALES AND YIELDS</h2> <div class="row"> <div class="col-md-9 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td>Run Scale</td> <td>Typical Yield for High-Copy, Stable Plasmids^</td> <td>Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^</td> </tr> <tr> <td>Midi</td> <td>70-100 μg</td> <td><span style="text-align: center;">10-50 μg</span> </td> </tr> <tr> <td>Maxi</td> <td><span style="text-align: center;">350-500 μg</span></td> <td><span style="text-align: center;">50-250 μg</span> </td> </tr> <tr> <td>Mega</td> <td><span style="text-align: center;">1.5-2.5 mg</span></td> <td><span style="text-align: center;">250 μg-1.25 mg</span> </td> </tr> <tr> <td>Giga</td> <td>7-10 mg</td> <td>1-5 mg</td> </tr> </tbody> </table> </div> </div> <div class="row"> <div class="col-md-9 col-centered"> <table class="table table-responsive table-bordered" style="text-align: center;"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td style="text-align: center;">Mini Preps</td> <td style="text-align: center;">Typical Yield for High-Copy, Stable Plasmids^</td> <td style="text-align: center;">Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^</td> </tr> <tr> <td style="text-align: center;">Tube</td> <td style="text-align: center;">7-10 μg</td> <td style="text-align: center;">2-5 μg</td> </tr> <tr> <td style="text-align: center;">96-well Plates</td> <td style="text-align: center;">5-10 μg</td> <td style="text-align: center;">1-3 μg</td> </tr> </tbody> </table> <p>^Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors. Please contact <a href="mailto:GS@azenta.com">GS@azenta.com</a> to discuss known low-copy, unstable, or toxic constructs.</p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="process-v1 Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="row"> <h2>Choice of Starting Material </h2> </div> <div class="row"> <div class="col-sm-4"> <img style="height: 110px; width: 210px;" class="img-responsive center-block" alt="Building" src="~/media/Images/Services/Plasmid/icons/110x210/dna_startingmaterial_110x210.ashx?h=110&la=en&w=210&hash=562C88D5588FAA4B7DFC1068FAE3D0C691F9356C&hash=562C88D5588FAA4B7DFC1068FAE3D0C691F9356C"> <h3>DNA</h3> <p>Transformation provided as a service<span style="color: #ff0000;">*</span></p> </div> <div class="col-sm-4"> <img style="height: 110px; width: 210px;" class="img-responsive center-block" alt="Building" src="~/media/Images/Services/Plasmid/icons/110x210/glycerol-stock_startingmaterial_110x210.ashx?h=110&la=en&w=210&hash=AE1077914CF9951C0A438718253A277D1BDC18E2&hash=AE1077914CF9951C0A438718253A277D1BDC18E2"> <h3>Glycerol Stock</h3> <p>Provide 200 μL frozen stock</p> </div> <div class="col-sm-4"> <img style="height: 110px; width: 210px;" class="img-responsive center-block" alt="Building" src="~/media/Images/Services/Plasmid/icons/110x210/bacterial-colony-startingmaterial_110x210.ashx?h=110&la=en&w=210&hash=16C5760CA290E6B2BA19088A39D1A2FC62630DDA&hash=16C5760CA290E6B2BA19088A39D1A2FC62630DDA"> <h3>Bacterial Colony</h3> <p>Please provide fresh colonies (<3 days old)</p> </div> </div> <div class="row margin-top-30"> <p class="text-left"><span style="color: #ff0000;">*</span>For DNA samples, Azenta Life Sciences, formerly GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.</p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner1 { width: 1170px; background-image: url('/-/media/10013-D_Customer-Referral_WebBanFull-Med_Background2.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner1 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner1-left { width: 60%; padding: 5px; } #banner1-right { width: 35%; padding: 15px; } #banner1-left h1 { text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-top: 15px; margin-bottom: 20px; color: #000000; } #banner1-left h3 { text-transform: none; 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margin: 0 auto; } } </style> <div style="width: 100%; background-color: #00000; padding: 20px; margin: 0;margin-top: -20px;"> <h2></h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Tech Note" src="/-/media/Plasmid-Prep-Tech-Note-Thumbnail.ashx?h=160&w=220&la=en&hash=F37CE1AD4FC30DCAB54FD0C2849F373956556CE4" /> </div> <div class="resource resource-right"> <h3>Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins</h3> <p> </p> <p>Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. 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