CRISPR gRNA Synthesis | CRISPR Construct Synthesis

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#f0f0f0;">CRISPR gRNA Synthesis service can synthesize the CRISPR sequence, clone it into your vector of choice, and perform sequence verification. CRISPR gene editing makes highly flexible, highly specific, and affordable genome engineering possible. Enabling almost any genomic target to be modified in any organism that can express exogenous DNA, CRISPR/Cas9 and other CRISPR-associated systems continue to revolutionize the field of genome engineering. This service is labeled as CRISPR Construct Synthesis in our <a href="https://clims4.genewiz.com/GeneSynthesis/CRISPR?lobId=2&serviceId=211" target="_blank">CLIMS4 ordering system</a>.</p> <a href="https://web.genewiz.com/gene-synth-inquiry?utm_source=crispr-validation" target="_blank"><button>Request Quote</button></a> </div> <div class="intro-content-box" id="intro-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q01hh0Zw0" target="_blank"><img alt="" src="/-/media/12035-D_mrNA-Launch_WebBanRight.ashx?la=en&hash=E713C2306C010F75724727ADB676A161BA686957" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is gRNA?</h2> <p>Guide RNA (gRNA) is an RNA sequence that serves to guide the editing process – directing the CRISPR associated nuclease (Cas nuclease) over to the target DNA region of interest.</p> <p> </p> <h2>What is synthetic sgRNA?</h2> <p>Synthetic sgRNA, or single guide RNA, is a synthetically generated single RNA molecule that contains the nucleotide sequence complementary to the target DNA (also known as CRISPR RNA or crRNA for short) and a tracrRNA that enables the Cas nuclease to bind.</p> <p> </p> <h2>What is the difference between sgRNA and gRNA?</h2> <p>The difference between sgRNA (single guide RNA) and gRNA (guide RNA) is that sgRNA contains both crRNA and tracrRNA fused into one RNA molecule. Meanwhile, gRNA (guide RNA) is a general term for all guide RNAs used with CRISPR. Synthetic sgRNA is better optimized for genome engineering compared to other guide RNAs, as it allows for fast, reliable, and reproducible editing results.</p> <p> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner3 { width: 1170px; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner3 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner3-left { width: 50%; padding: 20px; } #banner3-right { width: 49%; padding: 10px; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 5; margin-bottom: 0px; color: #FFFFFF } #banner3-right h3 { text-transform: none; letter-spacing: 1px; line-height: 29px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-left h3 { text-transform: none; letter-spacing: 1px; line-height: 29px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner3-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #826ec3; border-radius: 3px; transition: all .2s ease-in-out; font-size: 18px; font-weight: bold; } #banner3-left button { padding: 7px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #826ec3; border-radius: 3px; transition: all .2s ease-in-out; font-size: 17px; font-weight: bold; } #banner3-right button:hover { cursor: pointer; transform: scale(1.1); } #banner3-left button:hover { cursor: pointer; transform: scale(1.1); } #banner3-right button:focus { outline: 0; } #banner3-left button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner3 { width: 95%; height: auto; background-image: url('/-/media/12054-D_Gene-DIscovery-Solutions_WebBanFull-Med_BG.ashx'); display: inline-block; } #banner3-left { text-align: center; width: 100%; } #banner3-right { text-align: center; width: 100%; } #banner3-right h2 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner3-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner3-left img { width: auto; height: auto; } #banner3-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner3-left img { width: 60%; } #banner3-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner3"> <div id="banner3-left"> <h3 style="text-align:left;">STREAMLINE YOUR PATH TO DISCOVERY</h3> <h2 style="text-align:left;">Gene-to-Discovery Solutions</h2> <h3 style="text-align:left;">AAV Packaging, Custom mRNA Synthesis, Lentivirus Packaging, Recombinant Antibody Production</h3> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02gBwzs0" target="_blank"><button style="text-align:left;">LEARN MORE</button></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container margin-top-40"> <h2>gRNA Synthesis and Cloning</h2> <p class="text-left">Azenta offers very competitive pricing to synthesize, clone and sequence verify your gRNA expression cassettes into your preferred vector regardless of vector size or complexity.  Our strong expertise and experience in highly reliable synthetic construct generation services coupled with our CRISPR offering makes us a valuable partner to researchers around the globe. Simply provide your pre-design sequence to Azenta for any of the CRISPR-related products below and receive a quote within one business day: <br />  <br /> <strong>gRNA Synthesis and Cloning  </strong><br /> Azenta will synthesize and clone your gRNA sequences as cDNA into your preferred vector regardless of vector size and complexity, using the same industry-leading proprietary technology that Azenta has developed for standard gene synthesis products.<br />  <br /> <strong>Homology Directed Repair (HDR) Donor Construct and Custom Vector Synthesis </strong><br /> Need a custom donor or vector construct synthesized? Azenta has years of experience working with complex and unstable vectors.  We can provide custom constructs for your HR-mediated repair template as well as custom CRISPR vector generation and modifications. All custom vectors can be stored in-house for your future orders for up to 2 years at no additional cost.<br /> <a href="/en/Public/Services/Gene-Synthesis/Standard">Learn more ></a><br />  <br /> <strong>Standard Deliverables: </strong><br /> - 2-5 µg of lyophilized plasmid containing your sequence verified synthetic construct <br /> - Certificate of Analysis (COA) including restriction digest <br /> - Sequence trace data with alignment <br /> - Sequence files of synthetic gene alone and in vector <br />  <br /> <strong>Synthetic Libraries </strong><br /> Looking to create a gRNA library to screen knock-outs/knock-ins of a variety of genes in one experiment? Azenta custom <a href="https://www.genewiz.com/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries">synthetic library</a> service can create a custom gRNA library for your high-throughput CRISPR screening needs. </p> <p class="text-left"> </p> <p class="text-left"> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner { width: 1170px; background-image: url('/-/media/Images/13064-D_Lagator-Customer-Webinar_WebBanFull-Med_Desktop-BG.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 40%; padding: 20px; } #banner-right { width: 80%; padding: 10px; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #ffffff } #banner-right button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #FFFFFF; border: 2px solid #FFFFFF; border-radius: 3px; transition: all .2s ease-in-out; font-size: 18px; font-weight: bold; } #banner-right button:hover { cursor: pointer; transform: scale(1.1); } #banner-right button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .banner { width: 95%; height: auto; background: #294b62; display: inline-block; } #banner-left { text-align: center; width: 100%; } #banner-right { text-align: center; width: 100%; } #banner-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner"> <div id="banner-left"> <img alt="" src="-/media/.ashx" /></div> <div id="banner-right"> <div id="banner-right h1"> <h3>WEBINAR</h3> <h1>Understanding biological mechanisms to better predict the evolution of antibiotic resistance</h1> <div id="banner-right h3"> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/webinar/biological-mechanisms-predict-evolution-antibiotic-resistance?utm_campaign=2022-06 GEN NGS - New Customer Growth&utm_medium=web&utm_source=banner&utm_medium=CRISPR&__hstc=251210969.ee677d8374177d47808d4f65f2e7a318.1652957843001.1665408131811.1665479132940.197&__hssc=251210969.18.1665479132940&__hsfp=1345237511" target="_blank"><button>Watch Now</button></a> </div> </div> </div> </div> </div> </div> <div class="process-v1 Image-Slider Component-BackGround-Default margin-top-0 padding-top-10 padding-bottom-30"> <div class="container"> <div class="headline-center margin-bottom-20"> <div class="row"> <h2>TYPICAL CLONED GENE SYNTHESIS WORKFLOW</h2> </div> </div> <ul class="list-inline list-st row process-in headline-center"> <li style="text-align:center;cursor:pointer;" class="ImgSlili_1"> <div class="row headline-center"> <img src="/-/media/Images/Services/GS/workflow/day1.ashx?h=110&la=en&w=210&hash=E315552673A713AC14252895BBED2AD8A7D8DDF5" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Oligonucleotide Synthesis</h3> <br> </div> <div class="SeqIcon SeqIcon_1"> <span class="SeqIcon_text">1</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_1"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_2"> <div class="row headline-center"> <img src="/-/media/Images/Services/GS/workflow/day2.ashx?h=110&la=en&w=210&hash=08D4C9C92D97391DADB4052DA06E1FC02CA8C3D6" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Gene Assembly</h3> <br> </div> <div class="SeqIcon SeqIcon_2"> <span class="SeqIcon_text">2</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_2"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_3"> <div class="row headline-center"> <img src="/-/media/Images/Services/GS/workflow/day3.ashx?h=110&la=en&w=210&hash=C6CD034D47D0A5331782E7A1E52019D8BD0D80FB" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Cloning</h3> <br> </div> <div class="SeqIcon SeqIcon_3"> <span class="SeqIcon_text">3</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_3"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_4"> <div class="row headline-center"> <img src="/-/media/Images/Services/GS/workflow/day4.ashx?h=110&la=en&w=210&hash=D86B1BE583926EE0711029FA7B5BA52BE966E66D" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Quality Control</h3> <br> </div> <div class="SeqIcon SeqIcon_4"> <span class="SeqIcon_text">4</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_4"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_5"> <div class="row headline-center"> <img src="/-/media/Images/Services/GS/workflow/day5.ashx?h=110&la=en&w=210&hash=F0BCE5064898B299C22E7FD9CEF56709DB645B98" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Synthetic Gene Shipment</h3> <br> </div> <div class="SeqIcon SeqIcon_5"> <span class="SeqIcon_text">5</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_5"> </li> </ul> </div> <script language="javascript" type="text/javascript"> var i = 1; var iconCount = 0; var icons; var iconContent; var Flag; jQuery(document).ready(function ($) { icons = $(".SeqIcon"); iconContent = $(".icon-content"); //hide the content if (iconContent != null && jQuery(".content_1").length > 0) { iconContent.each(function (i) { if (i > 0) { $(this).hide(); } }); } //change the icon status if (icons != null) { iconCount = icons.length; $(icons[0]).addClass("SeqIcon_Sel"); Flag = setInterval(function () { if (i == iconCount) { i = 0; } SwcRCA(i); i++; }, 2000); } $("li[class^=ImgSlili]").bind("mousemove", function () { clearInterval(Flag); jQuery("div[class^='row icon-content content_']").hide(); jQuery(".Img-arror").find("img").attr("src", "/assets/img/gwz/arrow.png"); jQuery(icons).removeClass("SeqIcon_Sel"); jQuery(".content_" + $(this).attr("class").split("_")[1]).show(); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).addClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); }); $("li[class^=ImgSlili]").bind("mouseleave", function () { i = 0; Flag = setInterval(function () { SwcRCA(i); i++; }, 2000); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).removeClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow.png"); }); }); function SwcRCA(i) { if (i == 0) { jQuery(icons[icons.length - 1]).removeClass("SeqIcon_Sel"); } else { jQuery(icons[i - 1]).removeClass("SeqIcon_Sel"); } jQuery("div[class^='row icon-content content_']").hide(); jQuery(icons[i]).addClass("SeqIcon_Sel"); jQuery(".content_" + (i + 1)).show(); //change the arrow color jQuery(".Img_" + (i)).find("img").attr("src", "/assets/img/gwz/arrow.png"); if (i != (icons.length - 1)) { jQuery(".Img_" + (i + 1)).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); } } </script> </div> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2 class="no-padding-bottom no-margin-bottom">HOW TO ORDER</h2> </div> <div class="row"> <p>*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. 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