Plasmid Preparation Sample Submission Guidelines - Resources

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onclick="location.href = '/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction'"> <div class="row headline-center margin-bottom-10"> <img src="/-/media/Images/Home/icons/Purple-Icons/Nucleic-Acid-Extraction_110x210_Purple.ashx?h=110&la=en&w=210&hash=9C72E25F36E011F6ACD2D7CFB169F12713A6596D" alt="" width="210" height="110" /> </div> <div class="row headline-center"> GLP Nucleic Acid Extraction </div> </div> <div class="col-md-3" style="cursor:pointer;padding-top:20px;padding-bottom:20px;" onclick="location.href = '/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview'"> <div class="row headline-center margin-bottom-10"> </div> <div class="row headline-center"> Biofluid Processing </div> </div> <div class="col-md-3" style="cursor:pointer;padding-top:20px;padding-bottom:20px;" onclick="location.href = '/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis'"> <div class="row headline-center margin-bottom-10"> </div> <div class="row headline-center"> CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup> </div> </div> </div> </div> </div> </li> </ul> </li> </ul> </div> </div> </div> </div>  </div>  <br /><br /> <div class="headline-center Rich-Text-Edit-Area"> <br /> <br /> <div class="container"> <h1 style="text-align: center;">Plasmid Preparation</h1> <h3 style="text-align: center;">Sample Submission Guidelines</h3> <div class="col-md-8 col-md-offset-2"> <table class="table"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td>Sample Type</td> <td>Sample Guideline</td> </tr> <tr> <td>Clonal Plasmid DNA</td> <td>5 µl at 200 ng/µl (minimum 100 ng)<br /> </td> </tr> <tr> <td>Colonies on agar</td> <td><3 days old; single and well isolated</td> </tr> <tr> <td>Clonal Agar stab samples</td> <td> agar stabs</td> </tr> <tr> <td>Clonal Glycerol stocks</td> <td>200 µl of frozen stock</td> </tr> <tr> <td>Frozen bacterial cell pellets</td> <td>varies by scale<br /> </td> </tr> </tbody> </table> </div> <div class="col-md-12 text-left"> <p style="text-align: left;"><strong><br /> </strong></p> <p><strong>Viral Plasmid Containing Inverted Terminal Repeat (ITR)</strong></p> <p>Be aware that sequencing ITR is challenging, so we request concentrated stocks of DNA. </p> <ul> <li>Optimal sequencing concentration for Viral Plasmid is 300 ng/µl (minimum 200 ng/µl). Please submit as much template as you are able, up to 10 µl but no less than 5 µl. </li> <li>Please include exact concentration in <em>Notes</em> field for each template. </li> <li>Ensure 260/230 ration of 1.8-2.2, as this protocol is particularly sensitive to organic contamination. </li> <li>Primer – Please provide 30 µl per reaction at 5 pmol/µl (5 µM) in a labeled 1.5 or 0.5 ml tube. </li> <li>Generate primers that bind between 150 bps and 350 bp from the ITR.</li> </ul> <p style="text-align: left;"><strong> </strong></p> <p style="text-align: left;"><strong>Contact</strong></p> <p style="text-align: left;">Azenta Life Sciences offers GENEWIZ DNA prep services at several of our labs, including San Francisco, New Jersey, Boston & San Diego.</p> <p><strong>GENEWIZ South San Francisco</strong><br /> 201 Haskins Way, Suite 100<br /> South San Francisco, CA 94080<br /> Tel: 510-705-1455<br /> 12pm-8pm</p> <p><strong>GENEWIZ Global Headquarters</strong><br /> 111 Corporate Boulevard<br /> South Plainfield, NJ 07080<br /> Tel: 908-222-0711<br /> 8am-8pm<strong></strong></p> <strong> </strong> <p><strong>GENEWIZ Waltham</strong><br /> 1432 Main Street, Suite #110<br /> Waltham, MA 02451<br /> Tel: 617-300-0184<br /> 10am-7pm</p> <p><strong>GENEWIZ La Jolla</strong><br /> 11099 North Torrey Pines Road, Suite 270<br /> La Jolla, CA 92037<br /> 10am-8pm (M-F)</p> <p style="text-align: left;"><strong>Direct Shipping (FedEx, UPS):</strong> </p> <p>If a dropbox is not available or if your starting material requires dry ice, please use an overnight delivery service such as FedEx or UPS. A printout of the online <em>Order Summary</em> page must be included with the material. </p> <p>All direct shipments should be sent to:</p> <p><strong>GENEWIZ MB</strong><br /> 115 Corporate Boulevard <br /> South Plainfield, NJ 07080 <br /> USA<br /> 908-222-0711</p> <p><strong>GENEWIZ Dropbox (if available):</strong></p> <p>Starting material that is stable at room temperature may be submitted via a GENEWIZ Dropbox where available. A printout of the online <em>Order Summary</em> page must be included with the material. Contact <a href="mailto:GS@azenta.com">GS@azenta.com</a> to request a dropbox pickup for your location or if you have any dropbox questions.</p> </div> <strong> </strong> <p> </p> </div> <strong> <br /> <br /> </strong> </div> <link rel="stylesheet" href="/assets/css/footers/footer-v1.css?v=1"> <div class="footer-v1"> <div class="footer"> <div class="container text-center"> <div class="row"> <div class="col-md-12"> <span class="icon-Solid-Science-tagline"> <p style="font-size:35px; text-align: center;color: white;">GENEWIZ from Azenta Life Sciences</p> <p style="text-align: center;"><a href="https://www.azenta.com/" target="_blank"><img src="https://web.azenta.com/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor_WhiteType_RGB.png" width="250"></a></p> </span> </div> </div> </div>  <div class="container text-center socialicons"> <div class="row"> <div class="col-md-12"> <a href="https://www.facebook.com/AzentaLifeSciences"></a>      <a 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Plasmid Preparation Sample Submission Guidelines - Resources - GENEWIZ