GENEWIZ | Plasmid Prep FAQs

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document, '//static.hotjar.com/c/hotjar-', '.js?sv='); </script>  <script src="https://cdn.cookielaw.org/scripttemplates/otSDKStub.js" type="text/javascript" charset="UTF-8" data-domain-script="0190a77c-46be-74e5-a65b-09fc07bbf439" ></script> <script type="text/javascript"> function OptanonWrapper() { } </script>   <p style="display:none;"><button id="ot-sdk-btn" class="ot-sdk-show-settings">CookieSettings</button></p>  <br /><br /> <div class="headline-center Rich-Text-Edit-Area"> <br> <br> <h1>Plasmid Prep FAQs</h1> </div> <div class="container FAQs"> <div class="row"> <h3>Service Options</h3> </div> <div class="row"> <div class="NoBorder panel-group acc-v1 container" id='Service-Options'> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Service-Options" href="#QA-1" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What type of plasmid preparation services do you offer?</div> </a> </div> <div id="QA-1" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Our plasmid prep includes a standard-scale service with mini to giga scale preps, and a targeted yield service to deliver specific quantities of transfection-grade DNA. We also offer 96-well-mini-preps, custom large-scale preps, and AAV plasmid preps for your specific project needs. Plasmid prep can also be ordered using bacterial colonies or glycerol stocks from a Sanger sequencing order. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Service-Options" href="#QA-2" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What type of analysis option do you offer?</div> </a> </div> <div id="QA-2" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> We offer a wide variety of analysis options with your plasmid prep order including DNA sequencing, restriction digest analysis, sterility testing, and much more. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Service-Options" href="#QA-3" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Why should I choose you for my plasmid prep needs?</div> </a> </div> <div id="QA-3" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> We have proven a steadfast commitment to superior service over the last two decades, becoming a global leader in genomics services. With plasmid DNA preparation, we offer rapid turnaround times starting at just 2 business days, convenient online ordering, and a strict QC process with a wide range of analysis options to ensure high-quality plasmids. </div> </div> </div> </div> </div> </div><div class="container FAQs"> <div class="row"> <h3>Completing the Order Form</h3> </div> <div class="row"> <div class="NoBorder panel-group acc-v1 container" id='Order-Form'> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Order-Form" href="#QA-26" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What is the difference between # of Aliquots and Custom Tubes?</div> </a> </div> <div id="QA-26" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <p><strong># of Aliquots:</strong> The table below shows how we aliquot your sample volumes into tubes. If you would like your sample aliquoted in any other way, by volume or yield, that can be done for you at an additional charge. Examples for custom aliquot requests are also listed. </p> <center> </center> <div class="row"> <div class="col-md-9 col-centered" style="text-align: center;"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-blue"> <tr class="panel-heading"> <td>Run Scale</td> <td>Sample Volumes</td> </tr> <tr> <td>Mini</td> <td>60μl mini tube or 96-well plate depending on order size</td> </tr> <tr> <td>Midi</td> <td>200μl in 2ml tube</td> </tr> <tr> <td>Maxi</td> <td>500μl in 2ml</td> </tr> <tr> <td>Mega</td> <td>1ml in 2ml tube</td> </tr> <tr> <td>Giga</td> <td>(1ml in 2ml tube) x2</td> </tr> <tr> <td>Normalizations</td> <td>1.7ml per tube 2ml (leaving room for expansion if freezing) or mini tubes (<200ul)</td> </tr> </tbody> </table> </div> </div> <br /> <p style="margin-left: 30px;"> <em>Example 1:</em> 50μl in one tube, the rest in a second tube.<br /> <em>Example 2:</em> Split the order into 5 tubes.<br /> <em>Example 3:</em> 50μg per tube. </p> <p><strong>Custom Tubes:</strong> If you would like your samples shipped back to you in specific tubes from your stock, that can be done for you at an additional charge. Simply include a stock of your tubes with your samples.</p> </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Order-Form" href="#QA-4" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Which cell line should I choose for transformation?</div> </a> </div> <div id="QA-4" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <p>The table below provides guidance on selecting the appropriate cell line based on factors such as expression system, target protein, and downstream applications. </p> <center> </center> <div class="row"> <div class="col-md-9 col-centered" style="text-align: center;"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-blue"> <tr class="panel-heading"> <td>Strain</td> <td>Best for</td> </tr> <tr> <td>DH5alpha</td> <td>Regular cloning and storage, uptake of large plasmids</td> </tr> <tr> <td>Top10</td> <td>Regular cloning and storage</td> </tr> <tr> <td>Stable</td> <td>Lentiviral and retroviral plasmids, plasmids prone to rearrangement</td> </tr> <tr> <td>AAV</td> <td>Proprietary cell strain used for our AAV-ITR plasmid production services, designed to maximize ITR stability</td> </tr> </tbody> </table> </div> </div> </div> </div> </div> </div> </div> </div><div class="container FAQs"> <div class="row"> <h3>Sample Submission</h3> </div> <div class="row"> <div class="NoBorder panel-group acc-v1 container" id='FAQ-Prep'> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-5" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>How much starting material should I send?</div> </a> </div> <div id="QA-5" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <br /> <ul> <li>Purified DNA: At least 5 µL of DNA at 200 ng/µL (minimum 100 ng). Additional charge applies.</li> <li>Agar plate: Fresh (<3 days old) plate with single, well isolated colonies. Customer may indicate the colony (or colonies) to use, if desired.</li> <li>Glycerol stock or media sample: At least 200 µL. We can streak colonies (additional charges apply) for isolation prior to starting the liquid culture upon request.</li> <li>Cell pellet: Ship frozen on dry ice (direct to us via overnight carrier), with an indication of the culture volume, media used, OD600 at harvest, and cell pellet mass.</li> <li>DNA on filter paper: This format is discouraged – please send as purified, wet DNA in a sealed tube via overnight shipping (or our courier, if available). Additional charge applies.</li> <li>Other samples: Please contact our <a href="mailto:MB@azenta.com?Subject=Custom Plasmid Prep Requirements">Technical Support</a> team to discuss custom requirements.</li> </ul> </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-6" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Can I send my own antibiotic?</div> </a> </div> <div id="QA-6" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> We routinely stock Carbenicillin (Carb), Kanamycin, Chloramphenicol, Ampicillin, Tetracycline, Streptomycin, Spectinomycin, and Zeocin. We also supply Gentamycin, Hygromycin, Apramycin, and Erythromycins but do not keep them on hand, so some lead time will be necessary. An automatic upgrade of Ampicillin to Carbenicillin is included at no added charge. Other antibiotics may be submitted by the customer with their samples. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-7" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>How old can the agar plates be?</div> </a> </div> <div id="QA-7" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <p>Fresher is better; < 3days old with well isolated colonies. Older plates can be freshly streaked for isolation upon request. Please note that this will add to the order timeline.</p> <p>Please use caution in shipping agar plates during the cold winter months. The agar media may freeze in transit, rendering the plate unusable. Glycerol stocks are recommended as a substitute format during this season.</p> </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-8" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Are there types of plates that are not accepted?</div> </a> </div> <div id="QA-8" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Our plasmid prep team accepts only BSL1 samples. Typical agar plates used for these strains, bearing well isolated colonies are usually acceptable. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-9" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What is the maximum plasmid size (kb) that can be handled?</div> </a> </div> <div id="QA-9" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Our Plasmid DNA Preparation service is for plasmids in the 3-15 kb range. We do not currently offer BAC or cosmid preparation services. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-10" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Are there plasmids that you do not accept?</div> </a> </div> <div id="QA-10" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> We do not currently offer BAC or cosmid preparation services. Plasmids requiring handling outside standard protocols are reviewed on a case-by-case basis. Additional charges may apply. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-11" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>If I send my own primers, how much should I send?</div> </a> </div> <div id="QA-11" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Please send at least 10 µL for each sequencing reaction using that primer. The primer concentration should be 5 µM. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-12" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What cells are recommended for more difficult DNA types?</div> </a> </div> <div id="QA-12" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <p>Larger plasmids (>10 kb) and plasmids containing toxic or unstable sequences (such as long terminal repeats (LTR) found in viral expression vectors) are likely to produce better results when paired with cell lines engineered to maintain these difficult sequences. </p> <p>We advise against using standard cloning strains for these plasmids, due to increased risk of lower yield and plasmid instability. We also suggest the selection of additional QC testing to verify unstable or toxic plasmids.</p> </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Sample-Submission" href="#QA-13" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Who should I contact with any specific questions about sample submission for my project?</div> </a> </div> <div id="QA-13" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Please contact our Project Management team at <a href="mailto:GS@azenta.com">GS@azenta.com</a> or 908.222.0711 ext. 3 with any questions or to discuss your project. </div> </div> </div> </div> </div> </div><div class="container FAQs"> <div class="row"> <h3>Turnaround Time and Troubleshooting</h3> </div> <div class="row"> <div class="NoBorder panel-group acc-v1 container" id='ABOUT-PRIMERS'> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Turnaround-Troubleshooting" href="#QA-14" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>When will I receive my plasmid preps?</div> </a> </div> <div id="QA-14" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Typical turnaround time is 2-4 business days, depending on the order options selected. Additional testing or repeats may extend the timeline. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Turnaround-Troubleshooting" href="#QA-15" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>How can I track the status of my plasmid prep order?</div> </a> </div> <div id="QA-15" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <br /> <ol> <li>Log into your account and view <em>My Orders</em>. From this main menu you can also see <em>Order Status</em> next to each individual tracking number. </li> <li>If additional help is needed, please contact our Project Management team at <a href="mailto:GS@azenta.com" style="color: #826ec3;">GS@azenta.com</a> or 908.222.0711 ext. 3 with any questions or to discuss your project.</li> </ol> </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Turnaround-Troubleshooting" href="#QA-16" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Can I reorder my plasmid prep from last month?</div> </a> </div> <div id="QA-16" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Upon request, we will store a sample of your starting material or final DNA for up to one year at no additional charge, to facilitate subsequent ordering. Please be sure to request this storage in your order comments and include the order tracking number when reordering preps. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Turnaround-Troubleshooting" href="#QA-17" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Can I order a DNA prep from my gene synthesis order?</div> </a> </div> <div id="QA-17" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Azenta Project Management stores a sample of your final DNA from a gene synthesis order for up to one year at no additional charge. To order preps from these projects, simply go to the gene synthesis results page, select the construct of interest in the checkbox, and click “submit prep order” to initiate an online plasmid prep order. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#Turnaround-Troubleshooting" href="#QA-18" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What can I do if my prep yield is low?</div> </a> </div> <div id="QA-18" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> <p>Our internal quality monitoring will trigger the automatic repeat of a plasmid DNA preparation when the yield is below a critical threshold, depending on the anticipated copy number for the plasmid. Yield variance above this threshold could be due to a variety of factors and may be intrinsic to the sample provided.</p> <p>Plasmid DNA preparation orders are processed by run. For low copy or other low yield plasmids, we recommend ordering a larger preparation scale or multiple preps, depending on the amount of final material required.</p> <p>To discuss troubleshooting or custom process modifications, please contact our Project Management team at <a href="mailto:GS@azenta.com" style="color: #826ec3;">GS@azenta.com</a> or 908.222.0711 ext. 3 with any questions or to discuss your project.</p> </div> </div> </div> </div> </div> </div><div class="container FAQs"> <div class="row"> <h3>GLP-level Plasmid Preparation</h3> </div> <div class="row"> <div class="NoBorder panel-group acc-v1 container" id='FAQ-Prep'> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#GLP-Level" href="#QA-19" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What type of plasmid preparation services do you offer?</div> </a> </div> <div id="QA-19" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Our Quality System is developed in accordance with applicable FDA GLP regulations described in Title 21, Part 58 of the Code of Federal Regulations and EPA GLP regulations described in Title 40, Part 160 of the Code of Federal Regulations. We offer GLP services including GLP Plasmid Prep. For more details, please contact us at 877-436-3949, ext. 3350 or <a href="mailto:Regulatory@Azenta.com">Regulatory@Azenta.com</a>. </div> </div> </div> </div> </div> </div><div class="container FAQs"> <div class="row"> <h3>AAV Plasmid Preparation</h3> </div> <div class="row"> <div class="NoBorder panel-group acc-v1 container" id='FAQ-Prep'> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#AAV-Plasmid" href="#QA-20" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>How is your AAV plasmid preparation service different from your standard-scale plasmid preparation service?</div> </a> </div> <div id="QA-20" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> AAV plasmid preparation is our newly developed, proprietary process to propagate AAV plasmids while preserving the integrity of ITR regions. The service includes pre- and post-prep QC steps that leverage our AAV-ITR Sanger sequencing capabilities, while regular plasmid preparation kits and protocols still risk the loss of ITR integrity. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#AAV-Plasmid" href="#QA-21" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What is the advantage of a 2-step QC process?</div> </a> </div> <div id="QA-21" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Pre-prep QC assesses your sample configuration for the presence of full-length intact or truncated ITRs, or a mixture of both. It allows selected propagation of intact ITR sub-population only, while post-prep QC ensures integrity of ITR regions in the final plasmids delivered to you. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#AAV-Plasmid" href="#QA-22" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What if my sample contains truncated ITRs or is a mixture of intact and truncated ITR sequences?</div> </a> </div> <div id="QA-22" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> If your sample contains only truncated ITRs, we can fix the mutation at an additional cost and prep the corrected plasmids. If a mixture of intact and truncated ITRs is present, we can separate the full-length intact ITR sub-population and propagate it. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#AAV-Plasmid" href="#QA-23" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>My samples have been sequence-confirmed by your AAV-ITR Sanger sequencing service. Will they undergo pre-prep QC?</div> </a> </div> <div id="QA-23" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Pre-prep QC can be skipped if you could provide the tracking number of your AAV-ITR Sanger sequencing order, or the .ab1 files associated with the current aliquot of your samples. Any new transformation and plasmid preparation on your end will require a pre-prep QC. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#AAV-Plasmid" href="#QA-24" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>What starting materials do you accept, and what is the minimum amount required?</div> </a> </div> <div id="QA-24" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Currently, we only accept plasmid DNA. 10 µl at 200-300 ng/µl is recommended (minimum 200 ng/µl), in order to account for ITR sequencing as well. Please contact our Project Management team at <a href="mailto:GS@azenta.com" style="color: #826ec3;">GS@azenta.com</a> or 908.222.0711 ext. 3 for possible options if you have insufficient sample quantities. </div> </div> </div> <div class="NoBorder panel panel-default"> <div class="panel-heading SingleBorder" style="text-align:left;"> <a class="accordion-toggle" data-toggle="collapse" data-parent="#AAV-Plasmid" href="#QA-25" style="color:gray;text-align:left;"> <div class="QA col-sm-1">Q:</div> <div>Does your AAV plasmid preparation service guarantee a minimum yield?</div> </a> </div> <div id="QA-25" class=" panel-collapse collapse NoBorder"> <div class="panel-body NoBorder"> <div class="QA col-sm-1">A:</div> Final yield is dependent on the copy number of your sample(s), and a minimum yield cannot be guaranteed. 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