RNA Sequencing | RNA-Seq | GENEWIZ from Azenta

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quantitatively. It can identify the full catalog of transcripts, precisely define gene structures, and accurately measure gene expression levels.</p> <p>Our RNA-Seq services provide unparalleled flexibility in analyzing different RNA species, including coding, non-coding, and small transcripts, from a wide range of starting material using long- or short-read sequencing. Various RNA-Seq service options are available, including total, small, and single-cell RNA-Seq with RNA-Seq data analysis from a wide range of starting materials using short- or long-read sequencing. Our U.S.-based processing and support provides the fastest and most reliable service for customers in North America.</p> <p>For RNA-Seq experiments using FFPE tissue or other low-quality samples, <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry" target="_blank">submit an inquiry</a> about our highly-sensitive RNA exome solution. </p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry?utm_source=rna-seq" target="_blank"><button>Request Quote</button></a>  </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q030dTky0" target="_blank"><img alt="" src="/-/media/13069-D_NGS-New-Price_WebBanRight-US.ashx?la=en&hash=BE8000A098DB77F729C5D366D776291442EA7471" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; 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margin: 0 auto; } .ngs-content-box1 { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #ngs-content-box-left1 { width: 70%; text-align: left; padding: 30px 0; } #ngs-content-box-right1 { width: 29%; text-align: center; } .ngs-content-box1 img { width: 400px; } .ngs-content-box1 button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; } .ngs-content-box button1:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .ngs-new-container1 { text-align: center; margin-top: 0; } .ngs-new-content1 { width: 90%; margin: 0 auto; } .ngs-new-content1 { display: inline-block; } #ngs-content-box-right1, #ngs-content-box-left1 { width: 100%; text-align: center; } #ngs-content-box-right1 { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .ngs-new-content1 { width: 90%; margin: 0 auto; } .ngs-content-box1 img { width: 350px; } } </style> <div class="ngs-new-container1"> <div class="ngs-new-content1"> <h2>RNA Extraction & Library Preparation</h2> <div class="ngs-content-box1" id="ngs-content-box-left1"> <div> </div> <p>Having performed over 220,000 sample extractions, our experienced scientists can extract RNA from over 30 standard and hundreds of custom samples types to ensure RNA integrity and quality.</p> <p>We also offer several library preparation protocols tailored to meet your project requirements. In addition to standard and stranded poly(A) selection methods, we offer rRNA depletion for FFPE, poor-quality, and ultra-low input samples containing as few as 10pg of RNA or just a few cells. We also offer optional controls such as unique molecular identifiers (UMIs) and ERCC (External RNA Controls Consortium) RNA spike-ins to improve RNA-Seq data quality and quantification accuracy. </p> </div> <div class="ngs-content-box1" id="ngs-content-box-right1"> <p><img alt="Sample Types" src="/-/media/13002-D-4-Sample-Types.ashx?h=200&w=200&la=en&hash=BF0D016DC73368066BB3D1566049C0911E3E23E5" style="width: 200px; height: 200px;" /></p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .rna-seq-services { width: 1170px; margin: 0 auto; text-align: center; margin-bottom: 50px; } .service-container { width: 100%; text-align: center; display: flex; justify-content: space-around; } .rna-seq-service { width: 32%; text-align: center; padding: 20px 0 75px 0; position: relative; } .rna-seq-service h3 { margin: 0; margin-bottom: 15px; } .rna-seq-service p { width: 90%; margin: 0 auto; /*margin-bottom: 20px;*/ line-height: 20px; padding: 0; } #rna-seq-service-1 { background: #f0f0f0; } #rna-seq-service-1 span { display: none; } #rna-seq-service-2 { background: #f0f0f0; } #rna-seq-service-3 { background: #f0f0f0; } #rna-seq-service-4 { background: #f0f0f0; } #rna-seq-service-5 { background: #f0f0f0; } #rna-seq-service-6 { background: #f0f0f0; } #rna-seq-service-7 { background: #826ec3; padding: 20px 0 20px 0; } #rna-seq-service-8 { background: none; } #rna-seq-service-7 p { color: #f0f0f0; } #rna-seq-service-7 h3 { color: #f0f0f0; padding: 0 10px; } .rna-seq-service-btn-1 { background: #826ec3; color: #ffffff; border: 2px solid #826ec3; padding: 10px 20px; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; position: absolute; left: 50%; bottom: 20px; margin-left: -60px; } .rna-seq-service-btn-1:hover { background: #6959a1; border: 2px solid #6959a1; color: #fff; padding: 10px 20px; } .rna-seq-service-btn-2 { background: #826ec3; color: #ffffff; border: 2px solid #826ec3; padding: 10px 20px; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; position: absolute; left: 47%; bottom: 20px; margin-left: -60px; } .rna-seq-service-btn-2:hover { background: #6959a1; border: 2px solid #6959a1; color: #fff; padding: 10px 20px; } .rna-seq-service-btn-3 { background: none; color: #f0f0f0; border: 2px solid #f0f0f0; padding: 10px 20px; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; position: absolute; left: 46%; bottom: 20px; margin-left: -60px; } .rna-seq-service-btn-3:hover { background: #f0f0f0; border: 2px solid #f0f0f0; color: #3a3a3a; padding: 10px 20px; } @media only screen and (max-width: 1170px) { .rna-seq-services { width: 90%; } .rna-seq-service-btn { font-size: 12px; padding: 10px 18px; } .rna-seq-service-btn-2 { font-size: 12px; padding: 10px 18px; } .rna-seq-service-btn-3 { font-size: 12px; padding: 10px 18px; } } @media only screen and (max-width: 768px) { .service-container { display: block; } .rna-seq-service { width: 100%; margin: 10px 0; padding: 10px 0 20px 0; } .rna-seq-service-btn-1 { position: static; left: 0; right: 0; margin: 0; padding: 10px 20px; } .rna-seq-service-btn-2 { position: static; left: 0; right: 0; margin: 0; padding: 10px 20px; } .rna-seq-service-btn-3 { position: static; left: 0; right: 0; margin: 0; padding: 10px 20px; } .rna-seq-service p { margin-bottom: 20px; } } </style> <div class="rna-seq-services" id="rna-seq-services"> <h2 style="text-transform: uppercase;">RNA Sequencing Services</h2> <div class="service-container"> <div class="rna-seq-service" id="rna-seq-service-1"> <div class="rna-seq-service-content"> <h3>Single-Cell RNA-Seq</h3> <p>Single-cell RNA sequencing analyzes gene expression at single-cell resolution for heterogeneous samples. The 10x Genomics<sup>®</sup> Chromium™ platform provides advanced transcriptional profiling of thousands of individual cells.</p> </div> <a href="/Public/Services/Next-Generation-Sequencing/Single-Cell-RNA-Seq" target="_blank"><button class="rna-seq-service-btn-1">Learn More</button></a> </div> <div class="rna-seq-service" id="rna-seq-service-2"> <div class="rna-seq-service-content"> <h3>Ultra-Low Input RNA-Seq</h3> <p>Ultra-low input RNA sequencing provides bulk expression analysis of samples containing as few as 10 pg of RNA or just a few cells. Recommended for sequencing cells after fluorescence-activated cell sorting (FACS) or immunoprecipitation (RIP-Seq).</p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/case-study/ultra-low-rna-seq-uncovering-cell-expression-profiles" target="_blank"><button class="rna-seq-service-btn-1">Learn More</button></a> </div> <div class="rna-seq-service" id="rna-seq-service-3"> <div class="rna-seq-service-content"> <h3>Long-Read Isoform Sequencing</h3> <p>Iso-Seq (isoform sequencing) reads full-length contiguous transcripts. Applications include genome annotation, gene fusion detection, novel transcript discovery, and alternative splicing analysis.</p> </div> <a href="/Public/Services/Next-Generation-Sequencing/Iso-Seq" target="_blank"><button class="rna-seq-service-btn-1">Learn More</button></a> </div> </div> <br /> <div class="service-container"> <div class="rna-seq-service" id="rna-seq-service-4"> <div class="rna-seq-service-content"> <h3>Standard RNA-Seq</h3> <p>Standard RNA sequencing is our most popular option for profiling gene expression, enabling the analysis of coding (mRNA) and long non-coding RNA (lncRNA).</p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://fs.hubspotusercontent00.net/hubfs/3478602/13002-SD-2 1221 RNA-Seq Technical Specs Sell Sheet.pdf" target="_blank"><button class="rna-seq-service-btn-2">Download Info</button></a> </div> <div class="rna-seq-service" id="rna-seq-service-5"> <div class="rna-seq-service-content"> <h3>Strand-Specific RNA-Seq</h3> <p>Strand-specific RNA sequencing provides gene expression information with relation to strand orientation. Used for genome annotation, analysis of anti-sense/overlapping transcripts, and novel transcript discovery.</p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://fs.hubspotusercontent00.net/hubfs/3478602/13002-SD-2 1221 RNA-Seq Technical Specs Sell Sheet.pdf" target="_blank"><button class="rna-seq-service-btn-2">Download Info</button></a> </div> <div class="rna-seq-service" id="rna-seq-service-6"> <div class="rna-seq-service-content"> <h3>Small RNA-Seq</h3> <p>Small RNA sequencing enables analysis of miRNA and other small RNA species. Provides small RNA discovery and profiling.</p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://fs.hubspotusercontent00.net/hubfs/3478602/13002-SD-2 1221 RNA-Seq Technical Specs Sell Sheet.pdf" target="_blank"><button class="rna-seq-service-btn-2">Download Info</button></a> </div> </div> <br /> <div class="service-container"> <div class="rna-seq-service" id="rna-seq-service-1"> <div class="rna-seq-service-content"> <h3>CLIA RNA-Seq</h3> <p>RNA-Seq services performed in a CAP/CLIA laboratory for clinical applications. Custom CLIA validations for specific assays.</p> <p> </p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/clia-inquiry" target="_blank"><button class="rna-seq-service-btn-2">Submit Inquiry</button></a> </div> <div class="rna-seq-service" id="rna-seq-service-7"> <div class="rna-seq-service-content"> <h3>Unsure of the best RNA-Seq solution for your project?</h3> <p>Use our step-by-step guide</p> <p> </p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/guide-to-rna-seq-ebook" target="_blank"><button class="rna-seq-service-btn-3">Interactive Guide</button></a> </div> <div class="rna-seq-service" id="rna-seq-service-8"> <div class="rna-seq-service-content"> </div> </div> </div> <br /> <p>Find the right NGS solution for your project using our <a href="/en/Public/Services/Next-Generation-Sequencing#solution">interactive selection tool</a>.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css2?family=Open+Sans:wght@300;400;600;700&display=swap" rel="stylesheet" /> <style> * { box-sizing: border-box; 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}); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0"> <div class="container"> <div class="headline-center"> <br> <h2>Features & Benefits</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 padding-top-10 '> <div class="row"> <img src="/-/media/Images/Superior-Data-Quality_210x110_purple.ashx?h=111&la=en&w=211&hash=DD699E20C2910384213003A56F021C37CF1DF7D3" alt="Building" width="211" height="111" /> </div> <div class="row"> <br /><strong>Superior Data Quality</strong> </div> <div class="row"> Exceeding manufacturer’s benchmarks </div> </div> </li>  <li class='col-sm-4'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 padding-top-10 '> <div class="row"> <img src="/-/media/Images/Fast_Turnaround_Time1.ashx?h=111&la=en&w=211&hash=6FE844684845CAE64112083E142177C1DF78962B" alt="Building" width="211" height="111" /> </div> <div class="row"> <br /><strong>Fast Turnaround</strong> </div> <div class="row"> Starting at 1 week for sequencing </div> </div> </li>  <li class='col-sm-4'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 padding-top-10 '> <div class="row"> <img src="/-/media/Icon_210x110_US-Based.ashx?h=111&la=en&w=211&hash=1FCC458C24C199FDA32D33D14D49FEB277DF77AD" alt="Building" width="211" height="111" title="US based processing" /> </div> <div class="row"> <br /><strong>US-Based Processing</strong> </div> <div class="row"> Sequencing and customer support in New Jersey </div> </div> </li>  </ul> <div class="row headline-center"> </div> </div> </div><script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); 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height: auto; background-image: url('/-/media/11030DqPCRdPCRWebBanFullMedDesktopBackground.ashx'); display: inline-block; } #banner1-left { text-align: center; width: 100%; } #banner1-right { text-align: center; width: 100%; } #banner1-right h1 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 15px; color: #000000 } #banner1-right h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #000000 } #banner1-left img { width: auto; height: auto; } #banner1-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner1-left img { width: 60%; } #banner1-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner1"> <div id="banner1-left"> <h3>NEW TO OUR PCR PORTFOLIO</h3> <h1>Digital PCR (dPCR)</h1> <h3>Detect your target sequences with unmatched accuracy</h3> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Molecular-Genetics/PCR-Solutions" target="_blank"><button style="text-align:left;">Explore dPCR</button></a> </div> <div id="banner-right1"> <div id="banner-right1 h1">  </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; 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} .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #f1f0f0; padding: 0; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/13002-D-3_RNA-UMI-ERCC_TechResource-Thumb.ashx?la=en&hash=F046C5351C4001F2EBD93AB0541EA8B7AE58D331" /> </div> <div class="resource resource-right"> <h3>Blog | How to Improve Your RNA-Seq Data with UMIs and ERCC RNA</h3> <p>Measuring gene expression with RNA-Seq is susceptible to bias and variability. In this blog post, learn how unique molecular identifiers (UMIs) and ERCC RNA spike-ins can help you generate more accurate gene expression data. <br /> <a href="https://www.azenta.com/learning-center/blog/how-improve-your-rna-seq-data-umis-and-ercc-rna?utm_campaign=2024-09 GEN NGS - RNA-Seq Expansion&utm_source=rna-seq&utm_medium=technical resource" target="_blank"> <button>Read Blog</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/13002_RNA-Seq-Ebook_WE-Thumbnail.ashx?la=en&hash=E84911E32B3085C8455D0F4B3A7D7C9AC1C3F0C6" /> </div> <div class="resource resource-right"> <h3>eBook | A Guide to RNA-Seq</h3> <p>There are multiple factors to consider when selecting which RNA-Seq technique is the right approach for your project. In this eBook, discover the what, why, and how of RNA-Seq, the most common types of assays and platforms, and uncover insights into how to select the best method to achieve optimal results for your research.<br /> <a href="https://hubs.ly/Q02NS_3_0" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/13040_BI-Workbook_WE-Thumbnail.ashx?la=en&hash=9367D8BE069ABFF205E77EAD00B7F5EC91CC0BB2" /> </div> <div class="resource resource-right"> <h3>Quick Start Guide | Exploring Bioinformatics for Genome and Transcriptome Sequencing Data</h3> <p>For those new to bioinformatics, analyzing massive amounts of NGS data can be a daunting task. Download GENEWIZ’s bioinformatics quick start guide to learn how to analyze whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) data with bioinformatics tools to reveal biological insights for your research. <br /> <a href="https://web.genewiz.com/quick-start-guide/bioinformatics-next-generation-sequencing?utm_campaign=2022 GEN NGS - Tech Inno - BI Workbook&utm_source=eu rna-seq&utm_medium=tech resources" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13057-D_Transcriptomics-Webinar_TechResource_Laptop.ashx?la=en&hash=C9D7CAA35F4E698EB28DFEE74F8ADB2FA09B5276" /> </div> <div class="resource resource-right"> <h3>Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond</h3> <p>With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings. <br /> <a href="https://hubs.ly/Q01d5TDk0" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/NGS_On_Demand_BI_Workshop_US_Web-Tech-Thumb.ashx?la=en&hash=226C82B1F390DB9C439E66822D9DDBDDA8FA97B2" /> </div> <div class="resource resource-right"> <h3>RNA-Seq Bioinformatics Workshop & Roundtable Discussion</h3> <p>RNA-Seq bioinformatics can be complex and difficult to decipher. To help make it more approachable, this workshop and roundtable discussion, led by GENEWIZ bioinformatics manager Brian Sereni, explores the bioinformatics pipeline, explains NGS results, and addresses common challenges and FAQs for RNA-Seq bioinformatics analysis. <br /> <a href="https://hubs.ly/Q0117pvV0" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/13062-D_EU-Customer-Webinar_TechResource-Thumb.ashx?la=en&hash=74618789C1F38480E447D08C9D580DFDB40EFE6C" /> </div> <div class="resource resource-right"> <h3>Webinar | Non-Classical Sources of Tumour-Specific Antigen in Checkpoint Inhibitor Response</h3> <p>Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response. <br /> <a href="https://web.genewiz.com/webinar/non-classical-sources-tumour-specific-antigen-checkpoint-inhibitor-response" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="Dead Cell Removal" src="/-/media/Images/Services/NGS/SingleCellAtac2020/Technote_NGS_Optimizing_Single_Cell_webpage.ashx?la=en&hash=783F771FB875E6AD33BE6500BB46E63AC105541B" /> </div> <div class="resource resource-right"> <h3>Tech Note | Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal</h3> <p>Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data. <br /> <a href="https://web.genewiz.com/single-cell-dcr-tech-note" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/Resources/Ultra-Low-Case-Study-Thumbnail.ashx?la=en&hash=0F49AAD6F7C7B546F9838EA7EB49EFA142CFA518" /> </div> <div class="resource resource-right"> <h3>Case Study | Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq</h3> <p>Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells. <br /> <a href="http://web.genewiz.com/ultra-low-input-case-study" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/13017_Single-Cell_CS-Thumbnail.ashx?la=en&hash=CA3435D516373193B8E9B2CD047EE05F0A59DDDA" /> </div> <div class="resource resource-right"> <h3>Case Study | Single-Cell RNA-Seq Analysis Identifies Rare Drug-Resistant Cancer Stem Cells</h3> <p>Cell populations are rarely homogeneous and synchronized in their characteristics. Standard RNA-Seq approaches are limited to reporting general expression levels thus omitting minor subpopulation profiles. This study highlights new single-cell RNA sequencing capabilities for identifying rare cells, characterizing their transcriptomes, and discovering potential biomarkers. <br /> <a href="http://web.genewiz.com/rna-seq-case-study" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13002-D-2_RNA-Thera_TechResource-Thumb.ashx?la=en&hash=3A3C71D229F4F04549CF9D64FDA9D744DF306D36" /> </div> <div class="resource resource-right"> <h3>Tech Note | Full-Length RNA-Seq: A Novel Method to Assess Sequence Integrity for RNA Therapeutics</h3> <p>Strict quality control is required to maintain the integrity of manufactured products for RNA therapies, but current assays often present limitations. Learn how the novel full-length RNA-Seq approach from GENEWIZ allows you to preserve the entire length of and effectively sequence the poly(A) tails of your mRNA products. <br /> <a href="https://web.genewiz.com/tech-note/full-length-rna-seq?utm_campaign=2022 GEN NGS - Pharma&utm_source=eu rna-seq&utm_medium=tech resources" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/Services/NGS/photos/Iso_Seq_Thumnail.ashx?la=en&hash=686EC12313ABEF5B8707052F472968B33C41EC30" /> </div> <div class="resource resource-right"> <h3>Tech Note | Isoform Sequencing on the PacBio Sequel® – Maximizing Output and Accuracy</h3> <p>Contiguous mRNA full-length sequencing (Iso-Seq) greatly simplifies genome annotation efforts and revolutionizes the discovery of novel RNA isoforms. This Tech Note discusses the advantages of the latest technologies combined with GENEWIZ’s optimized workflow, and how this increases output and accuracy. <br /> <a href="http://web.genewiz.com/iso-seq-tech-note-web" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/NGS_RNA-Seq_Article_Web-Tech-Thumb.ashx?la=en&hash=5D4E609EBC15EB1457D0F71FA3FEEE945C8E10FB" /> </div> <div class="resource resource-right"> <h3>Article | Which RNA-Seq Technique Should I Use?</h3> <p>With so many RNA sequencing assay types to choose from, how do you know which is best suited for your NGS project? In this article, we discuss the most common RNA-Seq approaches and what factors to consider when selecting the right one. <br /> <a href="https://blog.genewiz.com/which-rna-seq-technique-should-i-use" target="_blank"> <button>Read Article</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13008-D_HT-GEx_Web-Tech-Thumb.ashx?la=en&hash=665950DF0138FC7B750C09882A2B8B85727255CC" /> </div> <div class="resource resource-right"> <h3>Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening</h3> <p>High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, GENEWIZ discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery. <br /> <a href="https://web.genewiz.com/tech-note/high-throughput-gene-expression-screening-phenotypic-profiling" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <p> </p> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is RNA sequencing (RNA-Seq) used for?</h2> <p>RNA sequencing, or RNA-Seq, is used to identify the nucleotide sequence of the RNA strand and detect the quantity of RNA in a sample.</p> <p>RNA-Seq provides a deeper insight into the transcriptome of a cell— enabling discovery of novel transcripts and differential gene expression analysis—by generating count data. In analyzing the count data, researchers can quantify RNA fragments and associate RNA sequences to respective genes in the sample.</p> <h2>What is the process of RNA sequencing (RNA-Seq)?</h2> <p>The process of RNA-Seq starts by isolating and fragmenting the RNA strand, then undergoing reverse transcription— synthesizing complementary DNA (cDNA)— and adding nucleotides to an RNA strand while copying the RNA strand simultaneously using next generation sequencing (NGS) technology.</p> <h2>How does RNA sequencing (RNA-Seq) analysis work?</h2> <p>RNA sequencing (RNA-Seq) analysis works by using a reference genome or transcriptome to map sequencing reads, quantifying the expression levels of the individual genes and transcripts, and identifying target genes and transcripts that are differentially expressed between samples. Researchers can perform de novo transcriptome assembly for new species with RNA-Seq using low concentrations of a starting sample, and/or with low-quality sample solutions.</p> <p> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>GENEWIZ from Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Illumina"><img alt="Illumina" src="/-/media/hires-illumina-logo-rgb_new.ashx?w=200&la=en&hash=2DE7778E5877DA9F3D063A9FAF021DA575ECF223" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#PacBio"><img alt="PacBio" 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