Immunogenomics | GENEWIZ from Azenta

<!DOCTYPE html>    <html lang="en">  <head> <title>Immunogenomics | GENEWIZ from Azenta</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content=": Immunogenomics is the study of gene regulatory mechanisms and gene mutations involved in disease-associated immune responses."> <meta name="keywords" content="immunogenomics, immunosequencing, immunoseq, immune repertoire, immune repertoire sequencing, genewiz, azenta, azenta life sciences"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Next-Generation-Sequencing/Immunogenomics/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Next-Generation-Sequencing/Immunogenomics" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Next-Generation-Sequencing/Immunogenomics" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/Immunogenomics" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Next-Generation-Sequencing/Immunogenomics" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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repertoire analysis. This approach uncovers the composition of B-cell receptors (BCRs) and T-cell receptors (TCRs) generated through the recombination of the V(D)J loci, which is crucial to the adaptive immune system. </p> <p>For in vivo natural antibody populations, we offer single-cell sequencing for physical pairing of the VH/VL or α/β chains of each complex or bulk sequencing for non-physical pairing inferred through frequency analysis. For in vitro recombinant antibody populations, short- and long-read options are available for unpaired and paired analyses. Add custom bioinformatics to any configuration to comprehensively characterize the entire BCR or TCR repertoire.</p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry?utm_source=immunogenomics" target="_blank"><button>Request Quote</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery" target="_blank"><img alt="" class="desktop12" src="/-/media/13071-D_Precision-Antibody-Disc_WebBanRight.ashx?la=en&hash=C52229CD28B79E27C66EFBFFDAB0B69F96D63155" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <h3>Accelerating Antibody-Drug Conjugate (ADC)<br /> Discovery with High-Throughput rAb Screening</h3> <p>Thursday, September 19, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02Jnv9Y0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> .table-bordered tbody tr td { vertical-align: middle; } </style> <div class="container"> <h2>Immunosequencing Options</h2> <p>Our suite of immunogenomics services provide next generation sequencing solutions for natural and recombinant antibody populations to achieve high-throughput analysis of diverse populations. Options include physical pairing of the VH/VL or α/β chains of each complex and non-physical pairing inferred through frequency analysis. Find the optimal solution for your project below.</p> <div class="row"> <div class="col-md-9 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td rowspan="2">Antibody Discovery<br /> Platform </td> <td colspan="2">Method of Pairing Chain Sequences</td> <td rowspan="2">Sequence Target</td> <td rowspan="2">Applications</td> </tr> <tr class="panel-heading"> <td>Frequency<br /> Analysis</td> <td>Physical<br /> Pairing</td> </tr> <tr> <td><em>In vivo</em></td> <td style="background: #fffaef;"><a href="/Public/Services/Next-Generation-Sequencing/Immunogenomics/Immuno-Profiling" target="_blank"><strong>Immuno-Profiling</strong></a></td> <td style="background: #e4f6fe;"><a href="/Public/Services/Next-Generation-Sequencing/Immunogenomics/Immuno-Profiling" target="_blank"><strong>Single-Cell<br /> Immuno-Profiling</strong></a></td> <td> <p>B-cell receptors (BCR)</p> <p>T-cell receptors (TCR)</p> </td> <td> <p>BCR/TCR repertoire<br /> profiling</p> <p><em>In vivo</em> antibody<br /> discovery</p> <p>Hybridoma screening</p> </td> </tr> <tr> <td><em>In vitro</em></td> <td style="background: #fffaef;"><a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services" target="_blank"><strong>Short-Read Amplicon<br /> Sequencing</strong></a></td> <td style="background: #e4f6fe;"><a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Long-Read-Amplicon-Sequencing" target="_blank"><strong>Long-Read Amplicon<br /> Sequencing</strong></a></td> <td> <p>Antigen-binding<br /> fragments (Fab)</p> <p>Single-chain variable<br /> fragments (scFv)</p> </td> <td><em>In vitro</em> antibody<br /> discovery via surface<br /> display</td> </tr> </tbody> </table> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .service-details-container { width: 1170px; margin: 0 auto; text-align: center; display: table; } .servic-details-column p { padding: 0; } .service-details-column { display: table-cell; width: 50%; /*height: 1740px;*/ text-align: center; vertical-align: top; padding: 15px 0 40px 0; } .service-details-column button { border: none; background: #826ec3; color: #fff; font-size: 14px; font-weight: 200; padding: 6px 10px; cursor: pointer; margin-top: 10px; } .column-section { padding-top: 50px; } .column-section img { width: 100%; } .column-section h3 { margin: 0; } .column-section p { width: 80%; margin: 0 auto; padding: 0; } #column-left { background: #fffaef; } #column-right { background: #e4f6fe; } .related-services { margin-top: 30px; } .related-services-boxes { width: 80%; margin: 0 auto; border: 2px solid #ccc; margin-bottom: 10px; padding-bottom: 20px; background-color: #fff; } .service-column { display: inline-block; width: 49%; vertical-align: middle; /* text-align: left;*/ } .service-column img { width: 100px; } #service-image { width: 30%; } #service-description { width: 69%; text-align: left; } @media only screen and (max-width: 1170px) { .service-details-container { width: 100%; } } @media only screen and (max-width: 768px) { .service-details-column { display: block; width: 100%; } } </style> <h2>Immunosequencing Workflows</h2> <div class="service-details-container"> <div class="service-details-column" id="column-left"> <div class="column-section"> <h3>Immuno-Profiling</h3> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/Immuno-Profiling_workflow2x.ashx?la=en&hash=F237AF3E1939165C4BE754FEFB273C40CB6B511F" /> <p>Perform bulk analysis of V(D)J sequences at the RNA level.</p> <a href="/Public/Services/Next-Generation-Sequencing/Immunogenomics/Immuno-Profiling" target="_blank"><button>Learn More</button></a> </div> <br /> <br /> <div class="column-section" style="border-top: 5px solid #ffffff;"> <h3>Short-Read Amplicon Sequencing</h3> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/Short-Read_Amplicon_Sequencing_workflow2x.ashx?la=en&hash=C7F6F2079F8A00E3DEAB618302833D1A864DB8B0" /> <p>Assay the diversity of clones after bio-panning in surface display experiments. Heavy and light chains, amplified separately, are fully sequenced on the Illumina MiSeq platform. We offer the following NGS services for this approach:</p> </div> <div class="related-services"> <div class="related-services-boxes"> <div class="service-column" id="service-image"> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/amplicon_ez_standard2x.ashx?la=en&hash=87A54269234281E91921EB5EAA7EDCD58640A048" /> </div> <div class="service-column" id="service-description"> <h3>Amplicon-EZ</h3> <p>A cost-effective solution that provides 50,000 reads per sample with a fast turnaround time.</p> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Amplicon-EZ"><button>Learn More</button></a> </div> </div> <div class="related-services-boxes"> <div class="service-column" id="service-image"> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/amplicon_ez_standard2x.ashx?la=en&hash=87A54269234281E91921EB5EAA7EDCD58640A048" /> </div> <div class="service-column" id="service-description"> <h3>Custom Short-Read Amplicon Sequencing</h3> <p>Offers customizable solutions with flexible data outputs and multiplexing.</p> <a href="/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Custom-Short-Read-Amplicon-Sequencing"><button>Learn More</button></a> </div> </div> <div class="related-services-boxes"> <div class="service-column" id="service-image"> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/Asset_1.ashx?la=en&hash=B8E3BAAAAA901CE37D0B7303D6B3AF1CCF29D1CC" /> </div> <div class="service-column" id="service-description"> <h3>TargetGxOne™ </h3> <p>Ideal for projects requiring custom amplification and sequencing.</p> <a href="/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels/Custom-Targeted-Sequencing"><button>Learn More</button></a> </div> </div> </div> </div> <div class="service-details-column" id="column-right"> <div class="column-section"> <h3>Single-Cell Immuno-Profiling</h3> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/Single-Cell-Immuno-Profiling_workflow2x.ashx?la=en&hash=3BCBFB7986D1DE4F2842B22367888D629B45E5A5" /> <p>Pair BCR and TCR chains at single-cell resolution using 10x Genomics™ technology.</p> <a href="/Public/Services/Next-Generation-Sequencing/Immunogenomics/Immuno-Profiling" target="_blank"><button>Learn More</button></a> </div> <br /> <div class="column-section" style="border-top: 5px solid #ffffff;"> <h3>Long-Read Amplicon Sequencing</h3> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/Ultra-Long_Amplicon_Sequencing_workflow2x.ashx?la=en&hash=2C13CC4311E44F4777ACB2DBE75A995156C2D53B" /> <p>Analyze clonal diversity after bio-panning via long-read sequencing technology. Heavy and light chains amplified together in a single PCR product are contiguously sequenced using the PacBio Sequel® platform. This approach physically pairs the sequences of the two chains.</p> </div> <div class="related-services"> <div class="related-services-boxes"> <div class="service-column" id="service-image"> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/amplicon_ez_standard2x.ashx?la=en&hash=87A54269234281E91921EB5EAA7EDCD58640A048" /> </div> <div class="service-column" id="service-description"> <h3>Long-Read Amplicon Sequencing</h3> <p>Offers contiguous sequencing of premade amplicons up to 10 kb.</p> <a rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services/Long-Read-Amplicon-Sequencing" target="_blank"><button>Learn More</button></a> </div> </div> <div class="related-services-boxes"> <div class="service-column" id="service-image"> <img alt="" src="/-/media/Images/Services/NGS/Immunogenomics/Asset_1.ashx?la=en&hash=B8E3BAAAAA901CE37D0B7303D6B3AF1CCF29D1CC" /> </div> <div class="service-column" id="service-description"> <h3>TargetGxOne™ </h3> <p>Ideal for projects requiring custom amplification and sequencing.</p> <a href="/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels/Custom-Targeted-Sequencing"><button>Learn More</button></a> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div class="tech-resources-container"> <div style="width: 100%; background-color: #ffffff; padding: 0; margin: 0;margin-top: -20px;"> <h2 style="box-sizing: border-box; border-radius: 0px; font-family: 'Open Sans SemiBold', sans-serif; font-weight: normal; line-height: 33px; color: #585f69; margin-top: 5px; margin-bottom: 20px; font-size: 24px; text-shadow: none; text-transform: uppercase; position: relative; padding-bottom: 15px; border-bottom: 1px solid #f1f0f0; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; letter-spacing: normal; text-align: center; text-indent: 0px; white-space: normal; word-spacing: 0px; background-color: #ffffff;">TECHNICAL RESOURCES</h2> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/NGS_PBMC_TechNote_Web-Thumbnail.ashx?la=en&hash=CEF074513EBB67DF9491F5E01CBEC2B42FC40D60" /> </div> <div class="resource resource-right"> <h3>An Optimized Approach to PBMC Isolation and Cryopreservation</h3> <p>The quantity and quality of isolated cells are critical to the performance of functional assays. In this tech note, we discuss the challenges associated with PBMC isolation and offer a robust workflow for PBMC isolation and cryopreservation leading to reproducible, high-quality results. <br /> <a href="https://web.genewiz.com/pbmc-isolation-tech-note" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13008-D_HT-GEx_Web-Tech-Thumb.ashx?la=en&hash=665950DF0138FC7B750C09882A2B8B85727255CC" /> </div> <div class="resource resource-right"> <h3>Tech Note: Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening</h3> <p>High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery. <br /> <a href="https://web.genewiz.com/gene-expression-technote?utm_campaign=NGS HT-Gene Expression&utm_source=rna-seq&utm_medium=technical resources&utm_content=EU" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div 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