GENEWIZ | PCR + Sanger Services Sample Submission Guidelines

<!DOCTYPE html>    <html lang="en">  <head> <title>GENEWIZ | PCR + Sanger Services Sample Submission Guidelines</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="Sample submission guidelines for GENEWIZ PCR + Sanger services including nucleic acid extraction, cDNA and gDNA verification, and SNP/Mutation analysis."> <meta name="keywords" content="PCR, sanger, cDNA verification, gDNA verification, SNP/Mutation Analysis, nucleic acid extraction, molecular genetics, fragment analysis, 16s rRNA sequencing"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='https://web.azenta.com/cell-and-gene-therapy' style='font-size:17px'>Cell and Gene Therapy Solutions 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'/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis'"> <div class="row headline-center margin-bottom-10"> <img src="/-/media/Images/Home/icons/Purple-Icons/GLP-compliant-SNP-Mutation-Analysis_110x210_Purple.ashx?h=110&la=en&w=210&hash=FEC877C60D6900DA782A8BA4A89823B016285E0F" alt="" width="210" height="110" /> </div> <div class="row headline-center"> GLP SNP Mutation Analysis </div> </div> <div class="col-md-3" style="cursor:pointer;padding-top:20px;padding-bottom:20px;" onclick="location.href = '/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep'"> <div class="row headline-center margin-bottom-10"> <img src="/-/media/Images/Home/icons/Purple-Icons/GLP-compliant-plasmid-prep_110x210_Purple.ashx?h=110&la=en&w=210&hash=50C0A6163387BD975BB09982BC5B1DBDCFCD5404" alt="" width="210" height="110" /> </div> <div class="row headline-center"> GLP Plasmid Prep </div> </div> <div class="col-md-3" style="cursor:pointer;padding-top:20px;padding-bottom:20px;" onclick="location.href = '/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction'"> <div class="row headline-center margin-bottom-10"> <img src="/-/media/Images/Home/icons/Purple-Icons/Nucleic-Acid-Extraction_110x210_Purple.ashx?h=110&la=en&w=210&hash=9C72E25F36E011F6ACD2D7CFB169F12713A6596D" alt="" width="210" height="110" /> </div> <div class="row headline-center"> GLP Nucleic Acid Extraction </div> </div> <div class="col-md-3" style="cursor:pointer;padding-top:20px;padding-bottom:20px;" onclick="location.href = '/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview'"> <div class="row headline-center margin-bottom-10"> </div> <div class="row headline-center"> Biofluid Processing </div> </div> <div class="col-md-3" style="cursor:pointer;padding-top:20px;padding-bottom:20px;" onclick="location.href = '/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis'"> <div class="row headline-center margin-bottom-10"> </div> <div class="row headline-center"> CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup> </div> </div> </div> </div> </div> </li> </ul> </li> </ul> </div> </div> </div> </div>  </div>  <br /><br /> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> span.anchor { display: block; position: relative; top: -100px; visibility: hidden; } </style> <br /> <br /> <div class="container text-left"> <h1 class="text-center">PCR + Sanger (R&D, GLP)</h1> <h2 class="text-center">Sample Submission Guidelines</h2> <p class="text-center">View our Sample Submission <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://fs.hubspotusercontent00.net/hubfs/3478602/13022-M&G 0122 PCR+Sanger Best Practices Checklist.pdf" target="_blank'">Best Practices Checklist</a>.</p> <p style="font-size:15px; text-align:center;"> <a href="#qPCR-guides">qPCR/RT-qPCR</a> | <a href="#dPCR-guides">dPCR</a> | <a href="#Nucleic-guides">Nucleic Acid Extraction</a> | <a href="#Mutation-guides">SNP/Mutation Analysis</a> | <a href="#cDNA-guides">cDNA Verification | </a><a href="#gDNA-guides">gDNA Verification</a> | <a href="#Fragment-guides">Fragment Analysis | </a><a href="#rRNA-guides">16S rRNA and Fungal ITS Sequencing | </a><a href="#plasmid-guides">Plasmid Sequencing | </a><a href="#yeast-guides">Yeast Colony Sequencing | </a><a href="#dna-guides">Large-Scale DNA Preparation</a> | <a href="#shipment-guides">Shipment Instructions</a></p> <h3 style="text-align: center;"><span class="anchor" id="qPCR-guides"></span> qPCR/RT-qPCR</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP <br /> </th> </tr> <tr> <td>Fresh frozen tissue</td> <td>At least 2 mg (10 mg recommended)</td> <td>By inquiry</td> </tr> <tr> <td>Cell pellets</td> <td>At least 10<sup>4</sup> cells (10<sup>6</sup> cells recommended)</td> <td>By inquiry</td> </tr> <tr> <td> DNA</td> <td>At least 2 µg (5 µg recommended)</td> <td>By inquiry</td> </tr> <tr> <td>RNA or cDNA</td> <td>At least 2 µg; recommended quality for Total RNA samples is RIN<span style="background-color: #ffffff; color: #444444;">≥6 (5 µg recommended)</span></td> <td>By inquiry</td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="dPCR-guides"></span>dPCR</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP <br /> </th> </tr> <tr> <td>Fresh frozen tissue</td> <td>At least 2 mg (10 mg recommended)</td> <td>At least 2 mg (10 mg recommended)</td> </tr> <tr> <td>Cell pellets</td> <td>At least 10<sup>4</sup> cells (10<sup>6</sup> cells recommended)</td> <td>At least 10<sup>4</sup> cells (10<sup>6</sup> cells recommended)</td> </tr> <tr> <td>Whole blood</td> <td>DNA extraction: 2 tubes of 200ul (500ul/tube recommended)<br /> <br /> RNA extraction: 2 tubes of 2.5mL (whole blood, PAXgene Blood RNA Tube) </td> <td>DNA extraction: 2 tubes of 200ul (500ul/tube recommended)<br /> <br /> RNA extraction: 2 tubes of 2.5mL (whole blood, PAXgene Blood RNA Tube)</td> </tr> <tr> <td>FFPE samples</td> <td>2 slices per sample, 5-10 µm per slice/curl (≥4 FFPE slides recommended)</td> <td>2 slices per sample, 5-10 µm per slice/curl (≥4 FFPE slides recommended)</td> </tr> <tr> <td>Viral particles</td> <td>Cell-free viral particles: 1 x 10<sup>7</sup> to 1 x 10<sup>12</sup>/particles per ml, submit 1 ml, 2 vials preferred<br /> <br /> Frozen allantoic fluid: at least 1 ml</td> <td>Cell-free viral particles: 1 x 10<sup>7</sup> to 1 x 10<sup>12</sup>/particles per ml, submit 1 ml, 2 vials preferred<br /> <br /> Frozen allantoic fluid: at least 1 ml</td> </tr> <tr> <td>Swab</td> <td>2 tubes/sample, 1 swab/tube</td> <td>2 tubes/sample, 1 swab/tube</td> </tr> <tr> <td>DNA</td> <td>DNA concentration should be between 2-50 ng/µl (100ng recommended per target); A260/A280 and A260/A230 ratios should be at least 1.8 to ensure high-quality DNA</td> <td>DNA concentration should be between 2-50 ng/µl (100ng recommended per target); A260/A280 and A260/A230 ratios should be at least 1.8 to ensure high-quality DNA</td> </tr> <tr> <td>RNA or cDNA</td> <td>At least 2 µg; recommended quality for total RNA samples is RIN≥6 (5 µg recommended)</td> <td>At least 2 µg; recommended quality for total RNA samples is RIN≥6 (5 µg recommended)</td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="Nucleic-guides"></span>Nucleic Acid Extraction</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP <br /> </th> </tr> <tr> <td>Frozen mammalian cell pellet samples</td> <td>At least 1 X 10<sup>6</sup> to 1 x 10<sup>7</sup> cells per pellet*</td> <td>At least 5 x 10<sup>6</sup> cells per pellet (2 pellets preferred), recommended 1 x 10<sup>7</sup> cells per pellet*</td> </tr> <tr> <td>Frozen mammalian tissue</td> <td>At least 25 mg*</td> <td>At least 25 mg*</td> </tr> <tr> <td>FFPE Tissue (slides or curls)</td> <td>1-2 slices per sample, 5-10 µm per slice/curl</td> <td>Not available</td> </tr> <tr> <td>Blood samples</td> <td>200 µl blood</td> <td>200 µl blood</td> </tr> <tr> <td>Buccal swabs</td> <td>At least 1 per sample</td> <td>Not available</td> </tr> <tr> <td>Saliva</td> <td>Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)</td> <td>Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)</td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="Mutation-guides"></span>SNP/Mutation Analysis</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td>Genomic DNA samples</td> <td><span style="background-color: #ffffff; color: #444444;">≥60 ng per amplicon per sample, conc 20 ng/µl, in water, elution buffer, or low TE buffer*</span></td> <td>At least 200 ng per sample, conc 20 ng/µl*</td> </tr> <tr> <td>Frozen mammalian cell pellet samples</td> <td>At least 1 x 10<sup>6</sup> to 1 x 10<sup>7</sup> cells per pellet*</td> <td>At least 5 x 10<sup>6</sup> cells per pellet (2 pellets preferred), recommended 1 x 10<sup>7</sup> cells per pellet*</td> </tr> <tr> <td>Frozen mammalian tissue</td> <td>At least 25 mg*</td> <td>At least 25 mg*</td> </tr> <tr> <td>FFPE Tissue (slides or curls)</td> <td>1-2 slices per sample, 5-10 µm per slice/curl</td> <td>Work can be performed on already extracted material from this sample type</td> </tr> <tr> <td>Blood samples</td> <td>200 µl blood</td> <td>200 µl blood</td> </tr> <tr> <td>Yeast Colony</td> <td>Agar plate with single and well isolated colonies</td> <td>Agar plate with single and well isolated colonies</td> </tr> <tr> <td>Buccal swabs</td> <td>At least 1 per sample</td> <td>Not available </td> </tr> <tr> <td>Saliva</td> <td>Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)</td> <td>Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)</td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="cDNA-guides"></span>cDNA Verification</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td><span>RNA</span></td> <td><span>At least 5 µg*</span></td> <td><span>At least 5 µg*</span></td> </tr> <tr> <td>cDNA</td> <td>At least 200 ng per sample, conc 20 ng/µl*</td> <td>At least 200 ng per sample, conc 20 ng/µl*</td> </tr> <tr> <td>Frozen mammalian cell pellet samples</td> <td>At least 1 x 10<sup>6</sup> to 1 x 10<sup>7</sup> cells per pellet*</td> <td>At least 5 x 10<sup>6</sup> cells per pellet (2 pellets preferred), recommended 1 x 10<sup>7</sup> cells per pellet*</td> </tr> <tr> <td>Frozen mammalian tissue</td> <td>At least 25 mg*</td> <td>At least 25 mg*</td> </tr> <tr> <td>FFPE Tissue (slides or curls)</td> <td>1-2 slices per sample, 5-10 µm per slice/curl</td> <td>Work can be performed on already extracted material from this sample type</td> </tr> <tr> <td>Blood samples</td> <td>200 µl blood</td> <td>200 µl blood</td> </tr> <tr> <td>Viral particles</td> <td> <p>If cell free viral particles: 1 x 10<sup>7</sup> to 1 x 10<sup>12</sup>/particles per ml, submit 1 ml, 2 vials preferred*</p> <p> If frozen allantoic fluid: at least 1 ml*</p> </td> <td> <p>If cell free viral particles: 1 x 107 to 1 x 10<sup>12</sup>/particles per ml, submit 1 ml, 2 vials preferred*</p> <p>If frozen allantoic fluid: at least 1 ml*</p> </td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="gDNA-guides"></span>gDNA Verification</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td>Genomic DNA samples</td> <td><span style="background-color: #ffffff; color: #444444;">≥60 ng per amplicon per sample, conc 20 ng/µl, in water, elution buffer, or low TE buffer*</span></td> <td>At least 120 ng per sample, conc 20 ng/µl*</td> </tr> <tr> <td>Frozen mammalian cell pellet samples</td> <td>At least 1 x 10<sup>6</sup> to 1 x 10<sup>7</sup> cells per pellet*</td> <td>At least 5 x 10<sup>6</sup> cells per pellet (2 pellets preferred), recommended 1 x 10<sup>7</sup> cells per pellet*</td> </tr> <tr> <td>Frozen mammalian tissue</td> <td>At least 25 mg*</td> <td>At least 25 mg*</td> </tr> <tr> <td>FFPE Tissue (slides or curls)</td> <td>1-2 slices per sample, 5-10 µm per slice/curl</td> <td>Work can be performed on already extracted material from this sample type</td> </tr> <tr> <td>Blood samples</td> <td>200 µl blood</td> <td>200 µl blood</td> </tr> <tr> <td>Virus</td> <td> <p>If cell free viral particles: 1 x 10<sup>7</sup> to 1 x 10<sup>12</sup>/particles per ml, submit 1 ml, 2 vials preferred*</p> <p>If frozen allantoic fluid: at least 1 ml*</p> </td> <td> <p>If cell free viral particles: 1 x 10<sup>7</sup> to 1 x 10<sup>12</sup>/particles per ml, submit 1 ml, 2 vials preferred*</p> <p>If frozen allantoic fluid: at least 1 ml*</p> </td> </tr> <tr> <td>Buccal swabs</td> <td>At least 1 per sample</td> <td>Not available</td> </tr> <tr> <td>Saliva</td> <td>Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)</td> <td>Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)</td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="Fragment-guides"></span>Fragment Analysis</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td><span>Labeled PCR products</span></td> <td>1<span>0-20 µl equivalent of PCR product</span></td> <td> <span>Not available</span></td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="rRNA-guides"></span>16S rRNA and Fungal ITS Sequencing</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td>Bacterial colony on agar</td> <td>Agar plate with single and well isolated colonies</td> <td>Not available</td> </tr> <tr> <td>gDNA</td> <td>At least 60 ng per sample, conc 20 ng/µl, homogeneous samples only for this service</td> <td>Not available</td> </tr> <tr> <td>Glycerol stocks</td> <td>200 µl of frozen stock on dry ice, homogeneous samples that can be pelleted when thawed (≥10<sup>6 </sup>cells)</td> <td>Not available</td> </tr> <tr> <td>Agar stabs</td> <td>Agar stab with clonal sample</td> <td> <p>Not available</p> </td> </tr> <tr> <td>Cell Pellets</td> <td><span style="background-color: #ffffff; color: #444444;">≥</span>10<sup>6 </sup>cells, dry or resuspended in buffer</td> <td> <p>Not available</p> </td> </tr> <tr> <td>Fungal colony on agar</td> <td>Agar plate with single and well isolated colonies</td> <td> <p>Not available</p> </td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="plasmid-guides"></span>Plasmid Sequencing</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td>Plasmid DNA</td> <td><a href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines/template-discovery-services#sanger-sequence">Primer Walking</a></td> <td>At least 5 µg*<br /> If transforming- At least 500 ng</td> </tr> <tr> <td>Glycerol stock</td> <td><a href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines/template-discovery-services#sanger-sequence">Primer Walking</a></td> <td>>200 µl of frozen stock on dry ice, 2 stocks preferred*</td> </tr> <tr> <td>Bacterial colony on agar</td> <td><a href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines/template-discovery-services#sanger-sequence">Primer Walking</a></td> <td>Agar plate with single and well isolated colonies</td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="yeast-guides"></span>Yeast Colony Sequencing</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D </th> <th class="col-md-4">GLP </th> </tr> <tr> <td>Yeast Colonies</td> <td><span>Agar plate with single and well isolated colonies</span></td> <td><span>Agar plate with single and well isolated colonies</span></td> </tr> </tbody> </table> <br /> <h3 style="text-align: center;"><span class="anchor" id="dna-guides"></span>Large-Scale DNA Preparation</h3> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th class="col-md-4">Sample Type</th> <th class="col-md-4">R&D</th> <th class="col-md-4">GLP </th> </tr> <tr> <td>Plasmid DNA</td> <td><a href="/Public/Resources/Sample-Submission-Guidelines/Plasmid-Preparation-Sample-Submission-Guidelines">Preps</a></td> <td>At least 5 µg<br /> If transforming - At least 500 ng</td> </tr> <tr> <td>Glycerol stock</td> <td><a href="/Public/Resources/Sample-Submission-Guidelines/Plasmid-Preparation-Sample-Submission-Guidelines">Preps</a></td> <td>200 µl of frozen stock on dry ice, 2 stocks preferred*</td> </tr> <tr> <td>Bacterial colony on agar</td> <td><a href="/Public/Resources/Sample-Submission-Guidelines/Plasmid-Preparation-Sample-Submission-Guidelines">Preps</a></td> <td>Agar plate with single and well isolated colonies</td> </tr> </tbody> </table> <p>*Requires Dry-Ice Shipment</p> <br /> <h3 class="text-center"><span class="anchor" id="shipment-guides"></span>SHIPMENT INSTRUCTIONS FOR ALL R&D-LEVEL SERVICES</h3> <p style="text-align: left;"><strong>Recommended Packaging and Shipping Guidelines:</strong></p> <p style="text-align: left;">Please secure all samples for shipment as appropriate:</p> <ol> <li>≤ 36 samples: Please submit samples in 1.5 ml snap-lock microcentrifuge tubes or 8-strip tubes with securely fastened lids. Parafilm can be used to minimize risk to samples during shipment.</li> <li>>36 samples: Please submit samples arrayed on a 96-well plate, sealed with an appropriate adhesive plate seal and centrifuged to collect samples in the wells.</li> <li>Agar plates: Please ship agar plates in a sturdy container with the agar-side up to prevent condensation build-up. Secure agar plate lids with tape, rubber bands, or parafilm to minimize risk to samples during shipment.</li> <li>Glycerol stocks, RNA, and samples for extraction should be shipped using dry ice. Please consider the estimated delivery date of your shipment when determining the amount of dry ice to use.</li> </ol> <p style="text-align: left;"><strong>Note: GENEWIZ accepts purified genomic DNA or can extract genomic DNA from various sample types, including biosafety level 1 (BSL1) and 2 (BSL2) supplied sources. We do not accept samples that require a higher level of processing than BSL 2.</strong></p> <br /> <p style="text-align: left;">Direct Shipping (FedEx, UPS or World Carrier):</p> <p style="text-align: left;">If a Drop Box is not available, or if your starting material requires dry ice, please use an overnight delivery service such as FedEx, UPS, or DHL. A print-out of the Excel order page must be included with the material.</p> <br /> <p style="text-align: left;">All direct shipments should be sent to:<br /> GENEWIZ<br /> Attn: Molecular Genetics<br /> 115 Corporate Blvd<br /> South Plainfield, NJ 07080 </p> <br /> <p style="text-align: left;">GENEWIZ Drop Box (if available):<br /> Starting material that is stable at room temperature may be submitted via a GENEWIZ Drop Box (when available). A print out of the Excel order page must be included with the material. Contact <a href="mailto: dnaseq@genewiz.com">dnaseq@genewiz.com</a> to request a Drop Box pick-up for your location or if you have any Drop Box questions.</p> <br /> <br /> <h3 class="text-center">SHIPMENT INSTRUCTIONS FOR ALL GLP-COMPLIANT SERVICES</h3> <p style="text-align: left;">All shipments via FedEx, UPS or World Courier should be sent to:</p> <p style="text-align: left;"> Dry ice, ship to:<br /> Attn: GLP Services<br /> 111 Corporate Boulevard<br /> South Plainfield, NJ 07080<br /> USA </p> <p style="text-align: left;">Please include a copy of the Sample Registration Form.</p> </div> <p> </p> </div> <link rel="stylesheet" href="/assets/css/footers/footer-v1.css?v=1"> <div class="footer-v1"> <div class="footer"> <div class="container text-center"> <div class="row"> <div class="col-md-12"> <span class="icon-Solid-Science-tagline"> <p style="font-size:35px; text-align: center;color: white;">GENEWIZ from Azenta Life Sciences</p> <p style="text-align: center;"><a 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GENEWIZ | PCR + Sanger Services Sample Submission Guidelines