GENEWIZ from Azenta | Bacterial and Fungal Identification

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Spacer (ITS) sequencing respectively. These highly conserved regions are standard tools used to construct bacterial and fungal phylogenies and taxonomies. Azenta Life Sciences, formerly Azenta offers pre-developed PCR assays that cover essential regions within the 16S rRNA gene and the ribosomal ITS region used for amplification and Sanger sequencing of clonal samples.</p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/MBForm/RNA?lobId=10&serviceId=1001" target="_blank"><button>Place an Order</button></a> </div> <div class="gs-content-box" id="gs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02l4rmP0" target="_blank"><img alt="" src="/-/media/10013-D_Customer-Referral_WebBanRight2.ashx?la=en&hash=21BD9C4C09D7AFE28645FBB92782B71F584DE6BE" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> * { box-sizing: border-box; } .details-row { display: flex; width: 800px; margin: 0 auto } /* Create two equal columns that sits next to each other */ .details-column { flex: 50%; padding: 10px; text-align: left; /*height: 300px;*/ /* Should be removed. Only for demonstration */ } @media only screen and (max-width: 800px) { .details-row { display: block; width: 100%; } .details-column { display: inline-block; width: 100%; } } </style> <h2>16S/ITS <span style="text-transform: none;">r</span>RNA SERVICE DETAILS</h2> <div class="details-row"> <div class="details-column"> <ul> <li>PCR amplification directly from bacterial or fungal colonies </li> <li>Enzymatic purification of crude PCR product enhances sequencing quality</li> <li>Forward and reverse sequencing of the 16S rRNA gene and the ITS region</li> </ul> </div> <div class="details-column"> <ul> <li>Starting materials accepted: <ul> <li>Bacterial and fungal colonies</li> <li>Extracted gDNA</li> <li>Glycerol stocks</li> <li>Cell pellets</li> </ul> </li> </ul> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); 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Quality control: PCR product is observed by agarose gel electrophoresis. </div> </div><div class="row icon-content content_3"> <div class="col-sm-10"> Resulting PCR product is sequenced in both directions. </div> </div> </div> <script language="javascript" type="text/javascript"> var i = 1; var iconCount = 0; var icons; var iconContent; var Flag; jQuery(document).ready(function ($) { icons = $(".SeqIcon"); iconContent = $(".icon-content"); //hide the content if (iconContent != null && jQuery(".content_1").length > 0) { iconContent.each(function (i) { if (i > 0) { $(this).hide(); } }); } //change the icon status if (icons != null) { iconCount = icons.length; $(icons[0]).addClass("SeqIcon_Sel"); Flag = setInterval(function () { if (i == iconCount) { i = 0; } SwcRCA(i); i++; }, 2000); } $("li[class^=ImgSlili]").bind("mousemove", function () { clearInterval(Flag); jQuery("div[class^='row icon-content content_']").hide(); jQuery(".Img-arror").find("img").attr("src", "/assets/img/gwz/arrow.png"); jQuery(icons).removeClass("SeqIcon_Sel"); jQuery(".content_" + $(this).attr("class").split("_")[1]).show(); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).addClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); }); $("li[class^=ImgSlili]").bind("mouseleave", function () { i = 0; Flag = setInterval(function () { SwcRCA(i); i++; }, 2000); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).removeClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow.png"); }); }); function SwcRCA(i) { if (i == 0) { jQuery(icons[icons.length - 1]).removeClass("SeqIcon_Sel"); } else { jQuery(icons[i - 1]).removeClass("SeqIcon_Sel"); } jQuery("div[class^='row icon-content content_']").hide(); jQuery(icons[i]).addClass("SeqIcon_Sel"); jQuery(".content_" + (i + 1)).show(); //change the arrow color jQuery(".Img_" + (i)).find("img").attr("src", "/assets/img/gwz/arrow.png"); if (i != (icons.length - 1)) { jQuery(".Img_" + (i + 1)).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); } } </script> </div><div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Service Overview</h2> <div class="row"> <div class="col-md-9 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-blue"> <tr class="panel-heading"> <td> </td> <td>16S rRNA Sequencing</td> <td>ITS rRNA Sequencing</td> </tr> <tr> <td>Genomic Loci Sequenced</td> <td class="text-left" style="text-align: center; vertical-align: middle;">16S rRNA gene<br> (V1 to V9 regions)</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Internal Transcribed Spacer 1 and 2<br> (ITS 1 & 2)</td> </tr> <tr> <td>Purpose of Assay</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Bacterial Identification</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Fungal Identification</td> </tr> <tr> <td>Deliverables</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Chromatogram and Sequence Files<br> <span style="color: #ff0000;">New!</span> Optional Analysis Available</td> <td class="text-left" style="text-align: center; vertical-align: middle;">Chromatogram and Sequence Files</td> </tr> <tr> <td> </td> <td class="text-left" style="text-align: center; vertical-align: middle;"><a rel="noopener noreferrer" href="https://clims4.genewiz.com/MBForm/RNA?lobId=10&serviceId=1001" target="_blank"><button style="background: #0067b1; padding: 6px 10px; border: none; border-radius: 5px; color: #ffffff;">Place an Order</button></a></td> <td class="text-left" style="text-align: center; vertical-align: middle;"><a rel="noopener noreferrer" href="https://clims4.genewiz.com/MBForm/RNA?lobId=10&serviceId=1016" target="_blank"><button style="background: #0067b1; padding: 6px 10px; border: none; border-radius: 5px; color: #ffffff;">Place an Order</button></a></td> </tr> </tbody> </table> </div> </div> </div> <br> <p><span style="color: #ff0000;">New!</span> Alignment and analysis of 16S raw data now available. 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