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<h2 class="mb-1" style="font-size:1.1rem;">[Pharmacological and Pharmaceutical Sciences]</h2> <h3 class="mb-1" style="font-size:1rem;">Online ISSN : 1307-6892</h3> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1236</span> Mapping the Neurotoxic Effects of Sub-Toxic Manganese Exposure: Behavioral Outcomes, Imaging Biomarkers, and Dopaminergic System Alterations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Katie%20M.%20Clark">Katie M. Clark</a>, <a href="https://publications.waset.org/abstracts/search?q=Adriana%20A.%20Tienda"> Adriana A. Tienda</a>, <a href="https://publications.waset.org/abstracts/search?q=Krista%20C.%20Paffenroth"> Krista C. Paffenroth</a>, <a href="https://publications.waset.org/abstracts/search?q=Lindsey%20N.%20Brigante"> Lindsey N. Brigante</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20C.%20Colvin"> Daniel C. Colvin</a>, <a href="https://publications.waset.org/abstracts/search?q=Jose%20Maldonado"> Jose Maldonado</a>, <a href="https://publications.waset.org/abstracts/search?q=Erin%20S.%20Calipari"> Erin S. Calipari</a>, <a href="https://publications.waset.org/abstracts/search?q=Fiona%20E.%20Harrison"> Fiona E. Harrison</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Manganese (Mn) is an essential trace element required for human health and is important in antioxidant defenses, as well as in the development and function of dopaminergic neurons. However, chronic low-level Mn exposure, such as through contaminated drinking water, poses risks that may contribute to neurodevelopmental and neurodegenerative conditions, including attention deficit hyperactivity disorder (ADHD). Pharmacological inhibition of the dopamine transporter (DAT) blocks reuptake, elevates synaptic dopamine, and alleviates ADHD symptoms. This study aimed to determine whether Mn exposure in juvenile mice modifies their response to DAT blockers, amphetamine, and methylphenidate and utilize neuroimaging methods to visualize and quantify Mn distribution across dopaminergic brain regions. Male and female heterozygous DATᵀ³⁵⁶ᴹ and wild-type littermates were randomly assigned to receive control (2.5% Stevia) or high Manganese (2.5 mg/ml Mn + 2.5% Stevia) via water ad libitum from weaning (21-28 days) for 4-5 weeks. Mice underwent repeated testing in locomotor activity chambers for three consecutive days (60 mins.) to ensure that they were fully habituated to the environments. On the fourth day, a 3-hour activity session was conducted following treatment with amphetamine (3 mg/kg) or methylphenidate (5 mg/kg). The second drug was administered in a second 3-hour activity session following a 1-week washout period. Following the washout, the mice were given one last injection of amphetamine and euthanized one hour later. Using the ex-vivo brains, magnetic resonance relaxometry (MRR) was performed on a 7Telsa imaging system to map T1- and T2-weighted (T1W, T2W) relaxation times. Mn inherent paramagnetic properties shorten both T1W and T2W times, which enhances the signal intensity and contrast, enabling effective visualization of Mn accumulation in the entire brain. A subset of mice was treated with amphetamine 1 hour before euthanasia. SmartSPIM light sheet microscopy with cleared whole brains and cFos and tyrosine hydroxylase (TH) labeling enabled an unbiased automated counting and densitometric analysis of TH and cFos positive cells. Immunohistochemistry was conducted to measure synaptic protein markers and quantify changes in neurotransmitter regulation. Mn exposure elevated Mn brain levels and potentiated stimulant effects in males. The globus pallidus, substantia nigra, thalamus, and striatum exhibited more pronounced T1W shortening, indicating regional susceptibility to Mn accumulation (p<0.0001, 2-Way ANOVA). In the cleared whole brains, initial analyses suggest that TH and c-Fos co-staining mirrors behavioral data with decreased co-staining in DATT356M+/- mice. Ongoing studies will identify the molecular basis of the effect of Mn, including changes to DAergic metabolism and transport and post-translational modification to the DAT. These findings demonstrate that alterations in T1W relaxation times, as measured by MRR, may serve as an early biomarker for Mn neurotoxicity. This neuroimaging approach exhibits remarkable accuracy in identifying Mn-susceptible brain regions, with a spatial resolution and sensitivity that surpasses current conventional dissection and mass spectrometry approaches. The capability to label and map TH and cFos expression across the entire brain provides insights into whole-brain neuronal activation and its connections to functional neural circuits and behavior following amphetamine and methylphenidate administration. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=manganese" title="manganese">manganese</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20toxicology" title=" environmental toxicology"> environmental toxicology</a>, <a href="https://publications.waset.org/abstracts/search?q=dopamine%20dysfunction" title=" dopamine dysfunction"> dopamine dysfunction</a>, <a href="https://publications.waset.org/abstracts/search?q=biomarkers" title=" biomarkers"> biomarkers</a>, <a href="https://publications.waset.org/abstracts/search?q=drinking%20water" title=" drinking water"> drinking water</a>, <a href="https://publications.waset.org/abstracts/search?q=light%20sheet%20microscopy" title=" light sheet microscopy"> light sheet microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20resonance%20relaxometry%20%28MRR%29" title=" magnetic resonance relaxometry (MRR)"> magnetic resonance relaxometry (MRR)</a> </p> <a href="https://publications.waset.org/abstracts/194636/mapping-the-neurotoxic-effects-of-sub-toxic-manganese-exposure-behavioral-outcomes-imaging-biomarkers-and-dopaminergic-system-alterations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/194636.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">8</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1235</span> Comparative Study on Effectiveness and Safety of Oral Antidiabetic Medications in Patients with Type 2 Diabetes Mellitus in a Tertiary Care Hospital of Bangalore, South India - A Prospective Cohort Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shobha%20Rani%20R.%20Hiremath">Shobha Rani R. Hiremath</a>, <a href="https://publications.waset.org/abstracts/search?q=Keerthana%20R."> Keerthana R.</a>, <a href="https://publications.waset.org/abstracts/search?q=Madhuvan%20H.%20S."> Madhuvan H. S.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> BACKGROUND: Type 2 Diabetes is a chronic health condition where the body cannot effectively use the insulin it produces, leading to elevated blood sugar levels It is often associated with lifestyle factors and insulin resistance. Globally, diabetes is on the rise, with urban areas like Bangalore seeing a surge due to lifestyle changes, stress, and dietary habits. To manage diabetes effectively, over 50 medications are available, each serving to regulate blood sugar through different mechanisms. This reflects the complex and individualized nature of diabetes treatment. Given the increase in medications for Type 2 diabetes mellitus, it is important to evaluate their effectiveness and safety so that clinicians can make informed choices while treating their patients. OBJECTIVES: To evaluate the effectiveness of various anti-diabetic medications used in the hospital in Type 2 diabetes patients by monitoring their HbA1c levels. To assess the safety of these medications by monitoring Adverse drug reactions if any. METHODOLOGY Design : Prospective Cohort study, Study period: 8 months, Sample Size: 100 patients, Inclusion Criteria: Patients above 18 years of both genders who were diagnosed with T2DM and who were prescribed oral hypoglycaemic agents. Exclusion Criteria: Diabetic patients with hepatic/renal failure, patients prescribed with insulin /not prescribed with OHAs and patients who were terminally ill, pregnant and lactating patients. Source of Data: Prescriptions, lab reports, ECG reports. Data collection forms were used to collect data which consisted of patient demographic details, drugs prescribed, laboratory investigations such as HbA1C, FBS, PPBS , ECG and any ADRs experienced. Data was collected at baseline, 3 months, and 6 months. It was statistically analyzed using SPSS (version 26) software. RESULTS: Greater number of patients (46%) were in the age group of greater than 61 years. 43 patients had no co-morbidities whereas 51 patients had Hypertension as the co morbidity. Basically 5 Drug combinations were prescribed as follows. Combination 1: Tablet Metformin HCL + Glimepiride (500, 2 mg) : 1-0-1, Combination 2: Tablet Sitagliptin + Metformin HCL (50, 500 mg) : 1-0-1, Combination 3: Tablet Vildagliptin + Metformin HCL (50, 500 mg): 1-0-1, Combination 4: Tablet Voglibose+ Glimepiride+ Metformin HCL (0.2 ,2 ,500mg): : 1-0-1, Combination 5: Tablet Voglibose+ Glimepiride+ Metformin HCL (0.2, 2 ,500mg): : 1-0-1 and T. Sitagliptin +Metformin HCL (50, 500 mg): 0-1-0. Combination 5 (Voglibose, Glimepiride, Metformin, Sitagliptin) was most effective in reducing HbA1c levels, showing a significant decrease (-0.00682, p = 0.001), followed by Combinations 4 and 3. However, Combination 5 also had the highest incidence of gastrointestinal side effects (72.7%) and ECG abnormalities (27.3%). Combination 1 (Metformin + Glimepiride) had the highest occurrence of hypoglycemia due to Glimepiride's insulin-stimulating effects. Weight loss was most notable in Combination 5, affecting 36.36% of patients. CONCLUSION: The enhanced effectiveness of Combinations 3, 4, and 5 suggests that a multi-drug approach that incorporates different mechanisms of action is more effective in managing HbA1c levels in patients with diabetes. Adverse effect profiles should be considered for personalized treatment strategies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=type%202%20diabetes" title="type 2 diabetes">type 2 diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=safety" title=" safety"> safety</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20anti%20diabetic%20medications" title=" oral anti diabetic medications"> oral anti diabetic medications</a>, <a href="https://publications.waset.org/abstracts/search?q=effectiveness" title=" effectiveness"> effectiveness</a> </p> <a href="https://publications.waset.org/abstracts/194580/comparative-study-on-effectiveness-and-safety-of-oral-antidiabetic-medications-in-patients-with-type-2-diabetes-mellitus-in-a-tertiary-care-hospital-of-bangalore-south-india-a-prospective-cohort-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/194580.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">9</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1234</span> Nitric Oxide and Potassium Channels but Not Opioid and Cannabinoid Receptors Mediate Tramadol-Induced Peripheral Antinociception in Rat Model of Paw Pressure Withdrawal</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Raquel%20R.%20Soares-Santos">Raquel R. Soares-Santos</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20P.%20Machado"> Daniel P. Machado</a>, <a href="https://publications.waset.org/abstracts/search?q=Thiago%20L.%20Romero"> Thiago L. Romero</a>, <a href="https://publications.waset.org/abstracts/search?q=Igor%20D.%20G.%20Duarte"> Igor D. G. Duarte</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Tramadol, an analgesic classified as an 'atypical opioid,' exhibits both opioid and non-opioid mechanisms of action. This study aimed to explore these mechanisms, specifically the opioid-, cannabinoid-, nitric oxide-, and potassium channel-based mechanisms, which contribute to the peripheral antinociception effect of tramadol, in an experimental rat model. The nociceptive threshold was determined using paw pressure withdrawal. To examine the mechanisms of action, several substances were administered intraplantarly: naloxone, a non-selective opioid antagonist (50 μg/paw); AM251 (80 μg/paw) and AM630 (100 μg/paw) as the selective antagonists for type 1 and type 2 cannabinoid receptors, respectively; nitric oxide synthase inhibitors L-NOArg, L-NIO, L-NPA, and L-NIL (24 μg/paw); and the enzyme inhibitors of guanylatocyclase and phosphodiesterase of cGMP, ODQ and zaprinast. Additionally, potassium channel blockers glibenclamide, tetraethylammonium, dequalinium, and paxillin were used. The results showed that opioid and cannabinoid receptor antagonists did not reverse tramadol’s effects. L-NOarg, L-NIO, and L-NPA partially reversed antinociception, while ODQ completely reversed, and zaprinast enhanced tramadol’s antinociception effect. Notably, glibenclamide blocked tramadol’s antinociception in a dose-dependent manner. These findings suggest that tramadol’s peripheral antinociception effect is likely mediated by the nitrergic pathway and sensitive ATP potassium channels, rather than the opioid and cannabinoid pathways. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=tramadol" title="tramadol">tramadol</a>, <a href="https://publications.waset.org/abstracts/search?q=nitric%20oxide" title=" nitric oxide"> nitric oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=potassium%20channels" title=" potassium channels"> potassium channels</a>, <a href="https://publications.waset.org/abstracts/search?q=peripheral%20analgesia" title=" peripheral analgesia"> peripheral analgesia</a>, <a href="https://publications.waset.org/abstracts/search?q=opioid" title=" opioid"> opioid</a> </p> <a href="https://publications.waset.org/abstracts/193887/nitric-oxide-and-potassium-channels-but-not-opioid-and-cannabinoid-receptors-mediate-tramadol-induced-peripheral-antinociception-in-rat-model-of-paw-pressure-withdrawal" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193887.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">9</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1233</span> Synthesis, Computational Studies, Antioxidant and Anti-Inflammatory Bio-Evaluation of 2,5-Disubstituted- 1,3,4-Oxadiazole Derivatives</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sibghat%20Mansoor%20Rana">Sibghat Mansoor Rana</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Islam"> Muhammad Islam</a>, <a href="https://publications.waset.org/abstracts/search?q=Hamid%20Saeed"> Hamid Saeed</a>, <a href="https://publications.waset.org/abstracts/search?q=Hummera%20Rafique"> Hummera Rafique</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Majid"> Muhammad Majid</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Tahir%20Aqeel"> Muhammad Tahir Aqeel</a>, <a href="https://publications.waset.org/abstracts/search?q=Fariha%20Imtiaz"> Fariha Imtiaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Zaman%20Ashraf"> Zaman Ashraf</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The 1,3,4-oxadiazole derivatives Ox-6a-f have been synthesized by incorporating flur- biprofen moiety with the aim to explore the potential of target molecules to decrease the oxidative stress. The title compounds Ox-6a-f were prepared by simple reactions in which a flurbiprofen –COOH group was esterified with methanol in an acid-catalyzed medium, which was then reacted with hydrazine to afford the corresponding hydrazide. The acid hydrazide was then cyclized into 1,3,4-oxadiazole-2-thiol by reacting with CS2 in the presence of KOH. The title compounds Ox-6a-f were synthesized by the reaction of an –SH group with various alkyl/aryl chlorides, which involves an S-alkylation reaction. The structures of the synthesized Ox-6a-f derivatives were ascer- tained by spectroscopic data. The in silico molecular docking was performed against target proteins cyclooxygenase-2 COX-2 (PDBID 5KIR) and cyclooxygenase-1 COX-1 (PDBID 6Y3C) to determine the binding affinity of the synthesized compounds with these structures. It has been inferred that most of the synthesized compounds bind well with an active binding site of 5KIR compared to 6Y3C, and especially compound Ox-6f showed excellent binding affinity (7.70 kcal/mol) among all synthesized compounds Ox-6a-f. The molecular dynamic (MD) simulation has also been performed to check the stability of docking complexes of ligands with COX-2 by determining their root mean square deviation and root mean square fluctuation. Little fluctuation was observed in case of Ox-6f, which forms the most stable complex with COX-2. The comprehensive antioxidant potential of the synthesized compounds has been evaluated by determining their free radical scavenging activity, including DPPH, OH, nitric oxide (NO), and iron chelation assay. The derivative Ox-6f showed promising results with 80.23% radical scavenging potential at a dose of 100 μg/mL while ascorbic acid exhibited 87.72% inhibition at the same dose. The anti-inflammatory activity of the final products has also been performed, and inflammatory markers were assayed, such as a thiobarbituric acid-reducing substance, nitric oxide, interleukin-6 (IL-6), and COX-2. The derivatives Ox-6d and Ox-6f displayed higher anti-inflammatory activity, exhibiting 70.56% and 74.16% activity, respectively. The results were compared with standard ibuprofen, which showed 84.31% activity at the same dose, 200 μg/mL. The anti-inflammatory potential has been performed by following the carrageen-induced hind paw edema model, and results showed that derivative Ox-6f exhibited 79.83% reduction in edema volume compared to standard ibuprofen, which reduced 84.31% edema volume. As dry lab and wet lab results confirm each other, it has been deduced that derivative Ox-6f may serve as the lead structure to design potent compounds to address oxidative stress. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=synthetic%20chemistry" title="synthetic chemistry">synthetic chemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceutical%20chemistry" title=" pharmaceutical chemistry"> pharmaceutical chemistry</a>, <a href="https://publications.waset.org/abstracts/search?q=oxadiazole%20derivatives" title=" oxadiazole derivatives"> oxadiazole derivatives</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-inflammatory" title=" anti-inflammatory"> anti-inflammatory</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-cancer%20compounds" title=" anti-cancer compounds"> anti-cancer compounds</a> </p> <a href="https://publications.waset.org/abstracts/193708/synthesis-computational-studies-antioxidant-and-anti-inflammatory-bio-evaluation-of-25-disubstituted-134-oxadiazole-derivatives" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193708.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">15</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1232</span> Pharmacokinetic Assessment of Antimicrobial Treatment of Acute Exacerbations of Chronic Obstructive Pulmonary Disease in Hospitalized Patients Colonized with Pseudomonas aeruginosa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Juliette%20Begin">Juliette Begin</a>, <a href="https://publications.waset.org/abstracts/search?q=Juliano%20Colapelle"> Juliano Colapelle</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrea%20Taratanu"> Andrea Taratanu</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Thirion"> Daniel Thirion</a>, <a href="https://publications.waset.org/abstracts/search?q=Amelie%20Marsot"> Amelie Marsot</a>, <a href="https://publications.waset.org/abstracts/search?q=Bryan%20A.%20Ross"> Bryan A. Ross</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chronic obstructive pulmonary disease (COPD), a leading cause of death globally, is characterized by chronic airflow obstruction and acute exacerbations (AECOPDs) that are often triggered by respiratory infections. Pseudomonas aeruginosa (P. aeruginosa), a potentially serious bacterial cause of AECOPDs, is treated with targeted anti-pseudomonal antibiotics. These select few antimicrobials are often used as first-line therapy in patients who are clinically unwell and/or in those suspected of P. aeruginosa-related infection prior to confirmation, potentially contributing to antimicrobial resistance. The present study evaluates prescribing practices in patients with a confirmed sputum history of P. aeruginosa admitted for AECOPD at the McGill University Health Centre (MUHC) and treated with anti-pseudomonal antibiotics. Serum antibiotic concentrations were measured from the same-day peak, trough, and mid-dose blood sampling intervals after reaching steady-state (on or after day 3) and were quantified using ultra-high-performance liquid chromatography (UHPLC). Demographic, clinical, and treatment outcomes were extracted from patient medical charts. Treatment failure was defined by respiratory-related death or mechanical ventilation after ≥3 days of antibiotics; antibiotic therapy extended beyond 2 weeks or a new antibiotic regimen started; or urgent care readmission within 30 days for AECOPD. To date, 9 of 30 planned participants have completed testing: seven received ciprofloxacin, one received meropenem, and one received piperacillin-tazobactam. Due to serum sample batching requirements, the serum ciprofloxacin concentration results for the first 2/8 participants are presented at the time of writing. The first participant had serum levels of 5.45mg/L (T₀), 4.74mg/L (T₅₀), and 4.49mg/L (T₁₀₀), while the second had serum levels of 5mg/L (T₀), 2.6mg/L (T₅₀), and 2.51mg/L (T₁₀₀). Pharmacokinetic parameters Cmax (5.18±0.43mg/L), T₁/₂ (23.56±18.94hours), and AUC (181.9±155.95mg*h/l) were higher than reported monograph values and met target AUC-to-MIC ratio of >125. The patients treated with meropenem and with piperacillin-tazobactam experienced treatment failure. Preliminary results suggest that standard ciprofloxacin dosing in patients experiencing an AECOPD and colonized with P. aeruginosa appears to achieve effective serum concentrations. Final cohort results will inform the pharmacokinetic appropriateness and clinical sufficiency of current AECOPD antimicrobial strategies in P. aeruginosa-colonized patients. This study will guide clinicians in determining the appropriate dosing for AECOPD treatment to achieve therapeutic levels, optimizing outcomes, and minimizing adverse effects. It could also highlight the value of routine antibiotic level monitoring in patients with treatment failure to ensure optimal serum concentrations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20exacerbation" title="acute exacerbation">acute exacerbation</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20resistance" title=" antimicrobial resistance"> antimicrobial resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=chronic%20obstructive%20pulmonary%20disease" title=" chronic obstructive pulmonary disease"> chronic obstructive pulmonary disease</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmacokinetics%2Fpharmacodynamics" title=" pharmacokinetics/pharmacodynamics"> pharmacokinetics/pharmacodynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=Pseudomonas%20aeruginosa" title=" Pseudomonas aeruginosa"> Pseudomonas aeruginosa</a> </p> <a href="https://publications.waset.org/abstracts/193317/pharmacokinetic-assessment-of-antimicrobial-treatment-of-acute-exacerbations-of-chronic-obstructive-pulmonary-disease-in-hospitalized-patients-colonized-with-pseudomonas-aeruginosa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193317.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">12</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1231</span> Assessment of Essential and Nonessential Metal Concentration in Selected Edible Fruit and Leaf Vegetables Grown with Adiahferom River, Tigray, Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mulugeta%20Gurum%20Gerechal">Mulugeta Gurum Gerechal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this piece of study, food safety questions and potential health risks make this as one of the most serious environmental concerns. Then, the levels of essential and non-essential heavy metals concentration were studied in Onion, Carrot, Swiss chard and Lettuce vegetables and compared the permissible levels with international guidelines for safe food. The concentration of Fe was found in the higher concentrations compared to other metals analyzed or significantly different at 95% confidence level than the rest metals studied in this study. However, the levels of the concentration of Cd and Pb exceeded the permissible level set by WHO specifications in water samples, Cd and Pb exceeded the permissible level set by FAO/WHO specifications in all vegetable samples collected from Adiahferom River Fe and Cu were also found below the recommended levels. The higher concentration of Pb and Cd above the permissible level in vegetables used for human food may pose health risk to consumer. However, the Fe hasn’t any health effect they take on from the Adiahferom body River. Mostly, the levels of metals in similar vegetable samples differed between the three sampling site, that may be due to variation in sources and processes of contaminations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adiahferom" title="Adiahferom">Adiahferom</a>, <a href="https://publications.waset.org/abstracts/search?q=turbidity" title=" turbidity"> turbidity</a>, <a href="https://publications.waset.org/abstracts/search?q=temperature" title=" temperature"> temperature</a>, <a href="https://publications.waset.org/abstracts/search?q=physico-chemical" title=" physico-chemical"> physico-chemical</a>, <a href="https://publications.waset.org/abstracts/search?q=assessment" title=" assessment"> assessment</a> </p> <a href="https://publications.waset.org/abstracts/193293/assessment-of-essential-and-nonessential-metal-concentration-in-selected-edible-fruit-and-leaf-vegetables-grown-with-adiahferom-river-tigray-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193293.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">8</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1230</span> Evaluation of Immune Responses of Gamma-Irradiated, Electron Beam Irradiated FMD Virus Type O/IRN/2007 Vaccines and DNA Vaccine- Based on the VP1 Gene by a Prime-Boost Strategy in a Mouse Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Farahnaz%20Motamedi%20Sedeh">Farahnaz Motamedi Sedeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Homayoon%20Mahravani"> Homayoon Mahravani</a>, <a href="https://publications.waset.org/abstracts/search?q=Parvin%20Shawrang"> Parvin Shawrang</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehdi%20Behgar"> Mehdi Behgar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Most countries use inactivated binary ethylenimine (BEI) vaccines to control and prevent Foot-and-Mouth Disease (FMD). However, this vaccine induces a short-term humoral immune response in animals. This study investigated the cellular and humoral immune responses in homologous and prime-boost (PB) groups in the BALB/c mouse model. FMDV strain O/IRN/1/2007 was propagated in the BHK-21 cell line and inactivated by three methods, including a chemical with BEI to produce a conventional vaccine (CV), a gamma irradiation vaccine (GIV), and an electron irradiated vaccine (EIV). Three vaccines were formulated with the adjuvant aluminum hydroxide gel. In addition, a DNA vaccine was prepared by amplifying the virus VP1 gene pcDNA3.1 plasmid. In addition, the plasmid encoding the granulocyte-macrophage colony-stimulating factor gene (GM-CSF) was used as a molecular adjuvant. Eleven groups of five mice each were selected, and the vaccines were administered as homologous and heterologous strategy prime-boost (PB) in three doses two weeks apart. After the evaluation of neutralizing antibodies, interleukin (IL)-2, IL-4, IL-10, interferon-gamma (INF-γ), and MTT assays were compared in the different groups. The pcDNA3.1+VP1 cassette was prepared and confirmed as a DNA vaccine. The virus was inactivated by gamma rays and electron beams at 50 and 55 kGy as GIV and EIV, respectively. Splenic lymphocyte proliferation in the inactivated vaccinated homologous groups was significantly lower (P≤0.05) compared with the heterologous prime-boosts (PB1, PB2, PB3) and DNA + GM-CSF groups (P≤0.05). The highest SNT titer was observed in the inactivated vaccine groups. IFN-γ and IL-2 were higher in the vaccinated groups. It was found that although there was a protective humoral immune response in the groups with inactivated vaccine, there was adequate cellular immunity in the group with the DNA vaccine. However, the strongest cellular and humoral immunity was observed in the PB groups. The primary injection was accompanied by DNA vaccine + GM-CSF and boosted injection with GIV or CV. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=foot%20and%20mouth%20disease" title="foot and mouth disease">foot and mouth disease</a>, <a href="https://publications.waset.org/abstracts/search?q=irradiated%20vaccine" title=" irradiated vaccine"> irradiated vaccine</a>, <a href="https://publications.waset.org/abstracts/search?q=immune%20responses" title=" immune responses"> immune responses</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20vaccine" title=" DNA vaccine"> DNA vaccine</a>, <a href="https://publications.waset.org/abstracts/search?q=prime%20boost%20strategy" title=" prime boost strategy"> prime boost strategy</a> </p> <a href="https://publications.waset.org/abstracts/193142/evaluation-of-immune-responses-of-gamma-irradiated-electron-beam-irradiated-fmd-virus-type-oirn2007-vaccines-and-dna-vaccine-based-on-the-vp1-gene-by-a-prime-boost-strategy-in-a-mouse-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193142.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">16</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1229</span> An Investigation of Etiology of Liver Cirrhosis and Its Complications with Other Co-morbid Diseases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tayba%20Akram">Tayba Akram</a> </p> <p class="card-text"><strong>Abstract:</strong></p> our main objective of this study is to work on the etiology of liver cirrhosis, to find basic reasons and causes of liver damage, and to find the pattern of liver cirrhosis in hepatic patients either suffering from hepatitis B/C or simple jaundice. We can evaluate medical treatment and the latest trends in patients suffering from liver cirrhosis. We can evaluate the side effects and adverse effects induced by drug therapy used to treat liver cirrhosis. The conclusion is based on the etiology of liver cirrhosis. The most common cause of liver cirrhosis is the viral Hepatitis C virus. Other common causes of liver cirrhosis that are estimated from our research are Hepatitis B virus, Diabetes Mellitus, Ascites, and very rarely found Hepatitis D virus. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=etiology" title="etiology">etiology</a>, <a href="https://publications.waset.org/abstracts/search?q=liver" title=" liver"> liver</a>, <a href="https://publications.waset.org/abstracts/search?q=cirrhosis" title=" cirrhosis"> cirrhosis</a>, <a href="https://publications.waset.org/abstracts/search?q=co-morbid%20diseases" title=" co-morbid diseases"> co-morbid diseases</a> </p> <a href="https://publications.waset.org/abstracts/193100/an-investigation-of-etiology-of-liver-cirrhosis-and-its-complications-with-other-co-morbid-diseases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193100.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">12</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1228</span> Spray-Dried, Biodegradable, Drug-Loaded Microspheres for Use in the Treatment of Lung Diseases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mazen%20AlGharsan">Mazen AlGharsan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: The Carbopol Microsphere of Linezolid, a drug used to treat lung disease (pulmonary disease), was prepared using Buchi B-90 nano spray-drier. Methods: Production yield, drug content, external morphology, particle size, and in vitro release pattern were performed. Results: The work was 79.35%, and the drug content was 66.84%. The surface of the particles was shriveled in shape, with particle size distribution with a mean diameter of 9.6 µm; the drug was released in a biphasic manner with an initial release of 25.2 ± 5.7% at 60 minutes. It later prolonged the release by 95.5 ± 2.5% up to 12 hours. Differential scanning calorimetry (DSC) revealed no change in the melting point of the formulation. Fourier-transform infrared (FT-IR) studies showed no polymer-drug interaction in the prepared nanoparticles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanotechnology" title="nanotechnology">nanotechnology</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20delivery" title=" drug delivery"> drug delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=Linezolid" title=" Linezolid"> Linezolid</a>, <a href="https://publications.waset.org/abstracts/search?q=lung%20disease" title=" lung disease"> lung disease</a> </p> <a href="https://publications.waset.org/abstracts/193025/spray-dried-biodegradable-drug-loaded-microspheres-for-use-in-the-treatment-of-lung-diseases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193025.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">13</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1227</span> Polyphenols: Isolation, Purification, Characterization and Evaluation of Various Biological Activities</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20Ijaz%20Hussain">Abdullah Ijaz Hussain</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The purpose of this study was to explore the cardioprotective and anti-inflammatory effects of polyphenol-rich extracts from cucurbitaceae family members, including Cucurbita pepo, C. moschata, and C. maxima, on rat models. The initial crude extracts from these cucurbits were further separated into hexane, chloroform, ethyl acetate, butanol, and aqueous ethanol fractions, labeled as HEF, CHF, EAF, BUF, and AEF, respectively. Of these, AEF yielded the highest amount, followed by BUF, HEF, EAF, and CHF in descending order. Notably, EAF contained the greatest concentration of total phenolics, flavonoids, and flavonols. In terms of antioxidant activity, EAF demonstrated the most potent DPPH radical scavenging capability, succeeded by CHF, BUF, AEF, and HEF. EAF also exhibited the strongest reducing potential among the fractions. RP-HPLC analysis identified various phenolic acids and flavonoids across the cucurbita fractions, including ferulic acid, vanillic acid, p-coumeric acid, gallic acid, p-hydroxybenzoic acid, chlorogenic acid, catechin, rutin, quercetin, myricetin, and kaempferol. Doses of 250 and 500 mg/kg body weight of cucurbita fractions were administered orally to male WKY rats daily for 21 days. The rats' body weight, heart rate, and blood pressure were monitored bi-weekly. Oxidative status assessments were conducted using plasma samples to measure levels of malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), nitric oxide (NO), and total antioxidant capacity (TAC). At the study's conclusion, surgical assessments, including blood pressure, pulse wave velocity (PWV), and echocardiograms (ECG) were performed. The findings indicated that EAF from cucurbita significantly enhanced antihypertensive and antioxidant activities in the SHR rat group. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title="polyphenols">polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=chlorogenic%20acid" title=" chlorogenic acid"> chlorogenic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=antihypertensive%20activity" title=" antihypertensive activity"> antihypertensive activity</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a>, <a href="https://publications.waset.org/abstracts/search?q=lcms" title=" lcms"> lcms</a> </p> <a href="https://publications.waset.org/abstracts/192459/polyphenols-isolation-purification-characterization-and-evaluation-of-various-biological-activities" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/192459.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">23</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1226</span> Evaluation of Mirabegron, Tolterodine, and Fesoterodine for Double-J Stent-Related Symptoms: A Comparative Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Janet%20Joy">Janet Joy</a>, <a href="https://publications.waset.org/abstracts/search?q=Shri%20Shailesh%20Amarkhed"> Shri Shailesh Amarkhed</a>, <a href="https://publications.waset.org/abstracts/search?q=Pradeep%20M.%20Kulkarni"> Pradeep M. Kulkarni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Ureteral stent-related symptoms significantly impact patients' quality of life. This study compared the efficacy of Mirabegron, Tolterodine, and Fesoterodine in managing these symptoms. Methodology: In this prospective, randomized, placebo-controlled trial, two hundred patients undergoing ureteral stenting were randomly assigned to receive Mirabegron, Tolterodine, Fesoterodine, or placebo for two weeks. Symptoms were assessed using the Ureteral Stent Symptom Questionnaire (USSQ) at stent removal. Results: 200 patients completed the study. Mirabegron demonstrated the lowest mean USSQ score (31.6 ± 6.4), followed by Fesoterodine (34.0 ± 6.9) and Tolterodine (35.0 ± 7.2), all significantly lower than placebo (48.6 ± 8.7, p<0.001). Mirabegron showed superior efficacy in reducing urinary symptoms (score: 16.5 ± 3.9) compared to Fesoterodine (17.8 ± 4.1) and Tolterodine (18.2 ± 4.3). Side effects, such as parched mouth, were less frequent with Mirabegron (6%) than with Tolterodine (28%) and Fesoterodine (24%). Conclusion: All three medications significantly improved stent-related symptoms compared to placebo. Mirabegron demonstrated a trend toward superior efficacy and fewer side effects, suggesting its potential as a first-line treatment for stent-related discomfort. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ureteral%20stent" title="ureteral stent">ureteral stent</a>, <a href="https://publications.waset.org/abstracts/search?q=mirabegron" title=" mirabegron"> mirabegron</a>, <a href="https://publications.waset.org/abstracts/search?q=tolterodine" title=" tolterodine"> tolterodine</a>, <a href="https://publications.waset.org/abstracts/search?q=fesoterodine" title=" fesoterodine"> fesoterodine</a>, <a href="https://publications.waset.org/abstracts/search?q=USSQ" title=" USSQ"> USSQ</a>, <a href="https://publications.waset.org/abstracts/search?q=stent-related%20symptoms" title=" stent-related symptoms"> stent-related symptoms</a> </p> <a href="https://publications.waset.org/abstracts/192339/evaluation-of-mirabegron-tolterodine-and-fesoterodine-for-double-j-stent-related-symptoms-a-comparative-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/192339.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">19</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1225</span> Viral Metagenomics Revealed a Novel Cardiovirus in Feces of Wild Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asif%20Mahmood">Asif Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Shama%20Shama"> Shama Shama</a>, <a href="https://publications.waset.org/abstracts/search?q=Hao%20Ni"> Hao Ni</a>, <a href="https://publications.waset.org/abstracts/search?q=Hao%20Wang"> Hao Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Ling"> Yu Ling</a>, <a href="https://publications.waset.org/abstracts/search?q=Hui%20Xu"> Hui Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Shixing%20Yang"> Shixing Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Qais%20Ahmad%20Naseer"> Qais Ahmad Naseer</a>, <a href="https://publications.waset.org/abstracts/search?q=Wen%20Zhang"> Wen Zhang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cardiovirus is a genus of viruses belonging to the family Picornaviridae. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China. Fecal samples were collected from 20 wild rats and pooled into four sample pools and then subjected to libraries construction which were then sequenced on Illumina MiSeq platform. The sequenced reads were analyzed using viral metagenomic analysis pipeline. A novel cardiovirus from feces of a wild rat was identified, named amzj-2018, of which the complete genome was acquired. Phylogenetic analysis based on the complete amino acid sequence of polyprotein revealed that amzj-2018 formed a separate branch located between clusters of Saffold virus and Rat Theilovirus 1 (RTV-1). Phylogenetic analysis based on different regions of the polyproteins, including P1, P2, P3, and P2+P3, respectively, showed discordant trees, where the tree based on P3 region indicated that amzj-2018 clustered separately between Theiler's murine encephalomyelitis virus and RTV-1. The complete genome of a cardiovirus was determined from the feces of wild rats which belonged to a novel type of cardiovirus based on phylogenetic analysis. Whether it is associated with disease needs further investigation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cardiovirus" title="cardiovirus">cardiovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=viral%20metagenomics" title=" viral metagenomics"> viral metagenomics</a>, <a href="https://publications.waset.org/abstracts/search?q=genomic%20organization" title=" genomic organization"> genomic organization</a>, <a href="https://publications.waset.org/abstracts/search?q=phylogenetic%20analysis" title=" phylogenetic analysis"> phylogenetic analysis</a> </p> <a href="https://publications.waset.org/abstracts/192230/viral-metagenomics-revealed-a-novel-cardiovirus-in-feces-of-wild-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/192230.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">18</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1224</span> Rationally Designed Dual PARP-HDAC Inhibitor Elicits Striking Anti-leukemic Effects</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amandeep%20Thakur">Amandeep Thakur</a>, <a href="https://publications.waset.org/abstracts/search?q=Yi-Hsuan%20Chu"> Yi-Hsuan Chu</a>, <a href="https://publications.waset.org/abstracts/search?q=Chun-Hsu%20Pan"> Chun-Hsu Pan</a>, <a href="https://publications.waset.org/abstracts/search?q=Kunal%20Nepali"> Kunal Nepali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The transfer of ADP-ribose residues onto target substrates from nicotinamide adenine dinucleotide (NAD) (PARylation) is catalyzed by Poly (ADP-ribose) polymerases (PARPs). Amongst the PARP family members, the DNA damage response in cancer is majorly regulated by PARP1 and PARP2. The blockade of DNA repair by PARP inhibitors leads to the progression of DNA single-strand breaks (induced by some triggering factors) to double-strand breaks. Notably, PARP inhibitors are remarkably effective in cancers with defective homologous recombination repair (HRR). In particular, cancer cells with BRCA mutations are responsive to therapy with PARP inhibitors. The aforementioned requirement for PARP inhibitors to be effective confers a narrow activity spectrum to PARP inhibitors, which hinders their clinical applicability. Thus, the quest to expand the application horizons of PARP inhibitors beyond BRCA mutations is the need of the hour. Literature precedents reveal that HDAC inhibition induces BRCAness in cancer cells and can broaden the therapeutic scope of PARP inhibitors. Driven by such disclosures, dual inhibitors targeting both PARP and HDAC enzymes were designed by our research group to extend the efficacy of PARP inhibitors beyond BRCA-mutated cancers to cancers with induced BRCAness. The design strategy involved the installation of Veliparib, an investigational PARP inhibitor, as a surface recognition part in the HDAC inhibitor pharmacophore model. The chemical architecture of veliparib was deemed appropriate as a starting point for the generation of dual inhibitors by virtue of its size and structural flexibility. A validatory docking study was conducted at the outset to predict the binding mode of the designed dual modulatory chemical architectures. Subsequently, the designed chemical architectures were synthesized via a multistep synthetic route and evaluated for antitumor efficacy. Delightfully, one compound manifested impressive anti-leukemic effects (HL-60 cell lines) mediated via dual inhibition of PARP and class I HDACs. The outcome of the western blot analysis revealed that the compound could downregulate the expression levels of PARP1 and PARP2 and the HDAC isoforms (HDAC1, 2, and 3). Also, the dual PARP-HDAC inhibitor upregulated the protein expression of the acetyl histone H3, confirming its abrogation potential for class I HDACs. In addition, the dual modulator could arrest the cell cycle at the G0/G1 phase and induce autophagy. Further, polymer-based nanoformulation of the dual inhibitor was furnished to afford targeted delivery of the dual inhibitor at the cancer site. Transmission electron microscopy (TEM) results indicate that the nanoparticles were monodispersed and spherical. Moreover, the polymeric nanoformulation exhibited an appropriate particle size. Delightfully, pH-sensitive behavior was manifested by the polymeric nanoformulation that led to selective antitumor effects towards the HL-60 cell lines. In light of the magnificent anti-leukemic profile of the identified dual PARP-HDAC inhibitor, in-vivo studies (pharmacokinetics and pharmacodynamics) are currently being conducted. Notably, the optimistic findings of the aforementioned study have spurred our research group to initiate several medicinal chemistry campaigns to create bifunctional small molecule inhibitors addressing PARP as the primary target. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=PARP%20inhibitors" title="PARP inhibitors">PARP inhibitors</a>, <a href="https://publications.waset.org/abstracts/search?q=HDAC%20inhibitors" title=" HDAC inhibitors"> HDAC inhibitors</a>, <a href="https://publications.waset.org/abstracts/search?q=BRCA%20mutations" title=" BRCA mutations"> BRCA mutations</a>, <a href="https://publications.waset.org/abstracts/search?q=leukemia" title=" leukemia"> leukemia</a> </p> <a href="https://publications.waset.org/abstracts/191592/rationally-designed-dual-parp-hdac-inhibitor-elicits-striking-anti-leukemic-effects" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/191592.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">23</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1223</span> Analyzing Healthy Eating Among Adolescent Teens Using the Socioecological Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kaavya%20Chandrasekar">Kaavya Chandrasekar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Healthy eating is essential to maintain good health and stable mental status regardless of age. WHO describes that a healthy diet consists of incorporating more fruits and vegetables and reducing the consumption of sugary and salty foods into a regularly scheduled healthy diet. Although this attitude is rather uncommon among all age groups, it is notably uncommon among the teens being a very vulnerable state in a man’s life. Faulty dietary habits, in the long run, interfere with health, leading to obesity, cardiovascular diseases, and mental instability. This study collates a discussion on the barriers prevailing among adolescents, to inculcate healthy eating practices by means of the socioecological model. The studies consisted of teens aged 13 to 19 years from schools and colleges of both sexes. The socio-ecological model emphasizes the interplay and interconnectedness of elements at all levels of health behavior, acknowledging that the majority of public health issues are just too complicated to be solved from a single-level perspective. As a result, it necessitates that people are not considered in isolation from bigger social groups. According to the studies retrieved from ten articles studies conducted globally, more than five articles suggest that socioeconomic class, lack of adult supervision and easy access to fast food stores and schools affect their decision of healthy eating. Awareness via personalized intervention has been tried and found successful. Future research is still needed to address various dimensions of the issue. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=socio%20ecological%20model" title="socio ecological model">socio ecological model</a>, <a href="https://publications.waset.org/abstracts/search?q=healthy%20eating" title=" healthy eating"> healthy eating</a>, <a href="https://publications.waset.org/abstracts/search?q=adolescents" title=" adolescents"> adolescents</a>, <a href="https://publications.waset.org/abstracts/search?q=fast%20food%20consumption" title=" fast food consumption"> fast food consumption</a>, <a href="https://publications.waset.org/abstracts/search?q=interventions." title=" interventions."> interventions.</a> </p> <a href="https://publications.waset.org/abstracts/191013/analyzing-healthy-eating-among-adolescent-teens-using-the-socioecological-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/191013.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">25</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1222</span> The Importance of Clinical Pharmacy and Computer Aided Drug Design</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mario%20Hanna%20Louis%20Hanna">Mario Hanna Louis Hanna</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The use of CAD (pc Aided layout) generation is ubiquitous inside the structure, engineering and construction (AEC) industry. This has led to its inclusion in the curriculum of structure faculties in Nigeria as an important part of the training module. This newsletter examines the moral troubles involved in implementing CAD (pc Aided layout) content into the architectural training curriculum. Using current literature, this study begins with the advantages of integrating CAD into architectural education and the responsibilities of various stakeholders in the implementation process. It also examines issues related to the terrible use of records generation and the perceived bad effect of CAD use on design creativity. The use of a survey technique, information from the architecture department of Chukwuemeka Odumegwu Ojukwu Uli college changed into accumulated to serve as a case observe on how the problems raised have been being addressed. The object draws conclusions on what guarantees a hit moral implementation. Tens of millions of human beings around the sector suffer from hepatitis C, one of the international's deadliest sicknesses. Interferon (IFN) is a remedy alternative for patients with hepatitis C, but these treatments have their aspect outcomes. Our research targeted growing an oral small molecule drug that goals hepatitis C virus (HCV) proteins and has fewer facet effects. Our contemporary study targets to broaden a drug primarily based on a small molecule antiviral drug precise for the hepatitis C virus (HCV). Drug improvement and the use of laboratory experiments isn't always best high-priced, however also time-eating to behavior those experiments. instead, on this in silicon have a look at, we used computational strategies to recommend a particular antiviral drug for the protein domain names of discovered in the hepatitis C virus. This examines used homology modeling and abs initio modeling to generate the 3-D shape of the proteins, then figuring out pockets within the proteins. Proper lagans for pocket pills were advanced the usage of the de novo drug design method. Pocket geometry is taken into consideration while designing ligands. A few of the various lagans generated, a different for each of the HCV protein domains has been proposed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20design" title="drug design">drug design</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-viral%20drug" title=" anti-viral drug"> anti-viral drug</a>, <a href="https://publications.waset.org/abstracts/search?q=in-silicon%20drug%20design" title=" in-silicon drug design"> in-silicon drug design</a>, <a href="https://publications.waset.org/abstracts/search?q=Hepatitis%20C%20virus%20%28HCV%29%20%20CAD%20%28Computer%20Aided%20Design%29" title=" Hepatitis C virus (HCV) CAD (Computer Aided Design)"> Hepatitis C virus (HCV) CAD (Computer Aided Design)</a>, <a href="https://publications.waset.org/abstracts/search?q=CAD%20education" title=" CAD education"> CAD education</a>, <a href="https://publications.waset.org/abstracts/search?q=education%20improvement" title=" education improvement"> education improvement</a>, <a href="https://publications.waset.org/abstracts/search?q=small-size%20contractor%20automatic%20pharmacy" title=" small-size contractor automatic pharmacy"> small-size contractor automatic pharmacy</a>, <a href="https://publications.waset.org/abstracts/search?q=PLC" title=" PLC"> PLC</a>, <a href="https://publications.waset.org/abstracts/search?q=control%20system" title=" control system"> control system</a>, <a href="https://publications.waset.org/abstracts/search?q=management%20system" title=" management system"> management system</a>, <a href="https://publications.waset.org/abstracts/search?q=communication." title=" communication."> communication.</a> </p> <a href="https://publications.waset.org/abstracts/189433/the-importance-of-clinical-pharmacy-and-computer-aided-drug-design" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189433.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">27</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1221</span> Curcumin Promotes the Deoxygenated State of Hemoglobin</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Roohallah%20Yousefi">Roohallah Yousefi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: In beta-thalassemia, an imbalance in the production of beta subunits of hemoglobin leads to the oxidation and deposition of excess alpha-globin chains at the cell membrane, resulting in the hemolysis of erythrocytes and a disorder of erythropoiesis. Antioxidants, such as curcumin, may promote this progression. This study aims to investigate the antioxidant effect of curcumin on hemolysate samples from patients with beta-thalassemia. Materials and methods: Pure curcumin was extracted and purified for use in studying its effect on the visual light absorbance of hemoglobin in hemolysate samples from beta-thalassemia patients compared to control samples. Changes in light absorbance at 540 and 700 nm wavelengths during exposure to curcumin were analyzed to examine the shift from oxyhemoglobin to deoxyhemoglobin. Results: Curcumin was found to dissolve rapidly and to a high degree in ethanol at 1 mg/ml, but did not dissolve in distilled water at the same concentration. The curcumin addition to the hemolysate sample of a patient with beta-thalassemia resulted in a decrease in the light absorbance of the sample at 540 nm wavelength, with minimal changes observed in the control sample. Conclusion: Curcumin deoxygenated the hemolysate samples from both the patient and control, causing hemoglobin precipitation to occur slowly. The study suggests a greater potential role for curcumin in deoxygenating hemoglobin in the hemolysate samples of beta-thalassemia patients compared to those of the normal control. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=beta-thalassemia" title="beta-thalassemia">beta-thalassemia</a>, <a href="https://publications.waset.org/abstracts/search?q=hemoglobin" title=" hemoglobin"> hemoglobin</a>, <a href="https://publications.waset.org/abstracts/search?q=curcumin" title=" curcumin"> curcumin</a>, <a href="https://publications.waset.org/abstracts/search?q=alpha-globin" title=" alpha-globin"> alpha-globin</a> </p> <a href="https://publications.waset.org/abstracts/189204/curcumin-promotes-the-deoxygenated-state-of-hemoglobin" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189204.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">29</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1220</span> Acute Poisoning Based on Age and Gender Caused by Pharmaceuticals and Therapies That Influence the Nervous System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ragy%20Raafat%20Gaber%20Attaalla">Ragy Raafat Gaber Attaalla</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: We looked at acute poisonings brought on by illegal drugs and pharmaceuticals that influence the nervous system at Assiut University Hospitals. Methods: Between January 2010 and December 2015, we conducted a retrospective examination of patient records from the largest tertiary toxicology referral center in Assiut. We examined the frequency, pattern, and distribution of ages and genders of acute nervous system agent poisoning. Results: 29,083 individuals total—16,657 (57.27%) males and 12,426 (42.73%) females—were included in the current study. Men's and women's median ages were 29 and 26, respectively (p < 0.0001). 10,326 (83.10%) women and 12,071 (72.47%) men under 40 were present (p < 0.001). 44.10% of cases had a history of poisoning, and the majority of cases (69.38% in men and 79.00% in women, p<0.001) were purposeful. Between various age groups and nervous system agents, there were notable variations in the ratios of men and women. The most often used agent for women was alprazolam, whereas methadone was more popular for men. Overall, there was a rising tendency in acute poisoning associated with alcohol and opioids used to treat addiction disorders, but a declining trend with benzodiazepines and antidepressants. Conclusion: Addiction to methadone was widespread, particularly in young males, and the majority of these cases were self-inflicted. Alprazolam and clonazepam poisoning most commonly affect women and males in the 20–29 age range, respectively. Opium was utilized by men over 30 and women over 60. Over half of the deaths were related to illicit narcotics, with opium being the most common. This research could raise awareness and lead to the development of gender- and age-specific local programs for education and prevention. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20poisonings" title="acute poisonings">acute poisonings</a>, <a href="https://publications.waset.org/abstracts/search?q=illegal%20drugs" title=" illegal drugs"> illegal drugs</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceuticals" title=" pharmaceuticals"> pharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=nerve%20system" title=" nerve system"> nerve system</a> </p> <a href="https://publications.waset.org/abstracts/189153/acute-poisoning-based-on-age-and-gender-caused-by-pharmaceuticals-and-therapies-that-influence-the-nervous-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189153.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">29</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1219</span> Unprecedented Bioactive Naturally-occurring Compounds from the Rare and Endangered Plants Endemic to China</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jin-Feng%20Hu">Jin-Feng Hu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Over the past decades, the global biodiversity has continued to decline. The threats to the terrestrial plant species have increased under anthropogenic activities and other massive ecological change impacts. The situation is much more serious in China, the third richest countries regarding plant biodiversity in the world. It was not until 1992 that the first volume of the China Plant Red Data Book was published. Nowadays, a significant number of Chinese endemic plants have been threatened (The IUCN Red List). Nevertheless, plant-originated natural products (NPs) have continued to play a crucial role in the drug discovery and development process. The opportunity for identifying new chemical entities for emerging and malignant diseases depends on a diversity of drug-producing species. Several statistical surveys unveiled that the rare and endangered plants (REPs) have proven to be better sources for drug discovery than other botanic sources. The identification of bioactive NPs from REPs reveals the importance of conservation efforts in preventing species diversity loss and addressing human diseases at the same time. Thus, there is an urgent need to investigate these fragile REPs. Since 2013, our group has initially launched a special program to systematically identify bioactive/novel NPs from REPs native to China. The selected plant species were generally collected from the remote Mountain areas, and have never been chemically or pharmacologically investigated. Due to the difficult collection of the mass-limited samples of REPs, studies on the secondary metabolites of REPs-associated endophytes would provide a promising alternative potential solution. This presentation details the achievements that related to a series of “Phytochemical and biological studies on rare and endangered plants endemic to China”. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioactive%20naturally-occrring%20compounds" title="bioactive naturally-occrring compounds">bioactive naturally-occrring compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=rare%20and%20endengered%20plants%20%28REPs%29" title=" rare and endengered plants (REPs)"> rare and endengered plants (REPs)</a>, <a href="https://publications.waset.org/abstracts/search?q=plant%20endophytes" title=" plant endophytes"> plant endophytes</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20discovery" title=" drug discovery"> drug discovery</a> </p> <a href="https://publications.waset.org/abstracts/188876/unprecedented-bioactive-naturally-occurring-compounds-from-the-rare-and-endangered-plants-endemic-to-china" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188876.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">33</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1218</span> Contribution of Artificial Intelligence in the Studies of Natural Compounds Against SARS-COV-2</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Salah%20Belaidi">Salah Belaidi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We have carried out extensive and in-depth research to search for bioactive compounds based on Algerian plants. A selection of 50 ligands from Algerian medicinal plants. Several compounds used in herbal medicine have been drawn using Marvin Sketch software. We determined the three-dimensional structures of the ligands with the MMFF94 force field in order to prepare these ligands for molecular docking. The 3D protein structure of the SARS-CoV-2 main protease was taken from the Protein Data Bank. We used AutoDockVina software to apply molecular docking. The hydrogen atoms were added during the molecular docking process, and all the twist bonds of the ligands were added using the (ligand) module in the AutoDock software. The COVID-19 main protease (Mpro) is a key enzyme that plays a vital role in viral transcription and mediating replication, so it is a very attractive drug target for SARS-CoV-2. In this work, an evaluation was carried out on the biologically active compounds present in these selected medicinal plants as effective inhibitors of the protease enzyme of COVID-19, with an in-depth computational calculation of the molecular docking using the Autodock Vina software. The top 7 ligands: Phloroglucinol, Afzelin, Myricetin-3-O- rutinosidTricin 7-neohesperidoside, Silybin, Silychristinthat and Kaempferol are selected among the 50 molecules studied which are Algerian medicinal plants, whose selection is based on the best binding energy which is relatively low compared to the reference molecule with binding affinities of -9.3, -9.3, -9, -8.9, -8 .5, 8.3 and -8.3 kcal mol-1 respectively. Then, we analyzed the ADME properties of the best7 ligands using the web server SwissADME. Two ligands (Silybin, Silychristin) were found to be potential candidates for the discovery and design of novel drug inhibitors of the protease enzyme of SARS-CoV-2. The stability of the two ligands in complexing with the Mpro protease was validated by molecular dynamics simulation; they revealed a stable trajectory in both techniques, RMSD and RMSF, by showing molecular properties with coherent interactions in molecular dynamics simulations. Finally, we conclude that the Silybin ligand forms a more stable complex with the Mpro protease compared to the Silychristin ligand. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=COVID-19" title="COVID-19">COVID-19</a>, <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20docking" title=" molecular docking"> molecular docking</a>, <a href="https://publications.waset.org/abstracts/search?q=ADME%20properties" title=" ADME properties"> ADME properties</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20dynamics" title=" molecular dynamics"> molecular dynamics</a> </p> <a href="https://publications.waset.org/abstracts/188747/contribution-of-artificial-intelligence-in-the-studies-of-natural-compounds-against-sars-cov-2" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188747.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">34</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1217</span> Methanolic Extract of the Exudates of Aloe Otallensis and Its Effect on Leishmania Donovani Parasite</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zerihun%20Tesfaye%20Nigusse">Zerihun Tesfaye Nigusse</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: This study evaluates the antileishmanial activity of the methanolic extract of Aloe otallensis (A. otallensis) on the promastigote stage of Leishmaniadonovani (L. donovani) as compared to standard drugs and to screen its phytochemical constituents. Methods: Phytochemical screening was done by using the method mentioned by Evans and Trease on methanolic extract of the exudates of Aloe otallensis leaves. The extract was also evaluated for in vitro antileishmanial activity against L. donavani, which is found in the Parasitology Unit of Black Lion Hospital. The result was compared to standard drugs of sodium stibogluconate, milfostin and paramomycin. Results: The extract has good antileishmanial activity with an IC50 of 0.123 0 μg/mL on L. donovani (AM 563). The experimental data showed that relatively, it had better activity than paramomycin and milfostin but less activity than sodium stibogluconate. The data analyses were done by GraphPad Prism version 5 software after it was read by an ELISA reader at the wavelength of 650 nm. The phytochemical screening of the exudates of A. otallensis showed the presence of phenol, alkaloid and saponin. Conclusions: The methanol extract of the exudates of A.otallensishas a good anti- leishmaniasis activity and this may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the conformation of which constituent presents in high concentration to know which one has the strongest effect. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti%20leshimaniasis" title="anti leshimaniasis">anti leshimaniasis</a>, <a href="https://publications.waset.org/abstracts/search?q=aloe%20otallensis" title=" aloe otallensis"> aloe otallensis</a>, <a href="https://publications.waset.org/abstracts/search?q=leshimania%20ethiopica" title=" leshimania ethiopica"> leshimania ethiopica</a>, <a href="https://publications.waset.org/abstracts/search?q=IC50" title=" IC50"> IC50</a> </p> <a href="https://publications.waset.org/abstracts/188744/methanolic-extract-of-the-exudates-of-aloe-otallensis-and-its-effect-on-leishmania-donovani-parasite" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188744.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">38</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1216</span> The importance of Clinical Pharmacy and Computer Aided Drug Design</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Peter%20Edwar%20Mortada%20Nasif">Peter Edwar Mortada Nasif</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The use of CAD (Computer Aided Design) technology is ubiquitous in the architecture, engineering and construction (AEC) industry. This has led to its inclusion in the curriculum of architecture schools in Nigeria as an important part of the training module. This article examines the ethical issues involved in implementing CAD (Computer Aided Design) content into the architectural education curriculum. Using existing literature, this study begins with the benefits of integrating CAD into architectural education and the responsibilities of different stakeholders in the implementation process. It also examines issues related to the negative use of information technology and the perceived negative impact of CAD use on design creativity. Using a survey method, data from the architecture department of Chukwuemeka Odumegwu Ojukwu Uli University was collected to serve as a case study on how the issues raised were being addressed. The article draws conclusions on what ensures successful ethical implementation. Millions of people around the world suffer from hepatitis C, one of the world's deadliest diseases. Interferon (IFN) is treatment options for patients with hepatitis C, but these treatments have their side effects. Our research focused on developing an oral small molecule drug that targets hepatitis C virus (HCV) proteins and has fewer side effects. Our current study aims to develop a drug based on a small molecule antiviral drug specific for the hepatitis C virus (HCV). Drug development using laboratory experiments is not only expensive, but also time-consuming to conduct these experiments. Instead, in this in silicon study, we used computational techniques to propose a specific antiviral drug for the protein domains of found in the hepatitis C virus. This study used homology modeling and abs initio modeling to generate the 3D structure of the proteins, then identifying pockets in the proteins. Acceptable lagans for pocket drugs have been developed using the de novo drug design method. Pocket geometry is taken into account when designing ligands. Among the various lagans generated, a new specific for each of the HCV protein domains has been proposed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20design" title="drug design">drug design</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-viral%20drug" title=" anti-viral drug"> anti-viral drug</a>, <a href="https://publications.waset.org/abstracts/search?q=in-silicon%20drug%20design" title=" in-silicon drug design"> in-silicon drug design</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatitis%20C%20virus" title=" hepatitis C virus"> hepatitis C virus</a>, <a href="https://publications.waset.org/abstracts/search?q=computer%20aided%20design" title=" computer aided design"> computer aided design</a>, <a href="https://publications.waset.org/abstracts/search?q=CAD%20education" title=" CAD education"> CAD education</a>, <a href="https://publications.waset.org/abstracts/search?q=education%20improvement" title=" education improvement"> education improvement</a>, <a href="https://publications.waset.org/abstracts/search?q=small-size%20contractor%20automatic%20pharmacy" title=" small-size contractor automatic pharmacy"> small-size contractor automatic pharmacy</a>, <a href="https://publications.waset.org/abstracts/search?q=PLC" title=" PLC"> PLC</a>, <a href="https://publications.waset.org/abstracts/search?q=control%20system" title=" control system"> control system</a>, <a href="https://publications.waset.org/abstracts/search?q=management%20system" title=" management system"> management system</a>, <a href="https://publications.waset.org/abstracts/search?q=communication" title=" communication"> communication</a> </p> <a href="https://publications.waset.org/abstracts/188179/the-importance-of-clinical-pharmacy-and-computer-aided-drug-design" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188179.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">22</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1215</span> A Study on How Domestic Cats' Nutritional Behavior is Affected by Adjustment Stress</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maria%20Magdy%20Danial%20Riad">Maria Magdy Danial Riad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The hypothalamic-pituitary-adrenal axis is activated by the adaptation stress, and this might result in the alteration of certain behavioral signs. The primary purpose of this paper is the adaptive stress effect on dietary behavior, which is directly correlated with changes in plasma cortisol levels. Physiological factors have a role in systems of adaptation and stress. Objectives: Ten clinically healthy cats were included in the study, and they were all kept in the same setting. Methods: On days 1, 5, 9, and 10 of the stay, each cat's behavior was observed through ethograms, and the serum cortisol levels were also measured at the same time. Significant behavioral changes in terms of nutrition were seen on the first day, with 50% of the participants not feeding and all participants not watering. Toward the study's conclusion, between days 5 and 9, there were no longer any discernible changes in the dietary habits, which might be attributed to the adaptation to the new living conditions. Cortisol variations in serological levels were consistent with behavioral changes; in 50% of the participants under observation, there was a substantial increase in values (p<0.05), which gradually declined as the study came to an end. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=domestic%20cats" title="domestic cats">domestic cats</a>, <a href="https://publications.waset.org/abstracts/search?q=ewes" title=" ewes"> ewes</a>, <a href="https://publications.waset.org/abstracts/search?q=nutritional%20behavior" title=" nutritional behavior"> nutritional behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=adjustment%20stress" title=" adjustment stress"> adjustment stress</a>, <a href="https://publications.waset.org/abstracts/search?q=plasma%20cortisol%20levels" title=" plasma cortisol levels"> plasma cortisol levels</a> </p> <a href="https://publications.waset.org/abstracts/187021/a-study-on-how-domestic-cats-nutritional-behavior-is-affected-by-adjustment-stress" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/187021.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">41</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1214</span> Screening of Phytochemicals Compounds from Chasmanthera dependens and Carissa edulis as Potential Inhibitors of Carbonic Anhydrases CA II (3HS4) Receptor using a Target-Based Drug Design</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Owonikoko%20Abayomi%20Dele">Owonikoko Abayomi Dele</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Epilepsy is an unresolved disease that needs urgent attention. It is a brain disorder that affects over sixty-five (65) million people around the globe. Despite the availability of commercial anti-epileptic drugs, the war against this unmet condition is yet to be resolved. Most epilepsy patients are resistant to available anti-epileptic medications thus the need for affordable novel therapy against epilepsy is a necessity. Numerous phytochemicals have been reported for their potency, efficacy and safety as therapeutic agents against many diseases. This study investigated 99 isolated phytochemicals from Chasmanthera dependens and Carissa edulis against carbonic anhydrase (ii) drug target. The absorption, distribution, metabolism, excretion and toxicity (ADMET) of the isolated compounds were examined using admet SAR-2 web server while Swiss ADME was used to analyze the oral bioavailability, drug-likeness and lead-likeness properties of the selected leads. PASS web server was used to predict the biological activities of selected leads while other important physicochemical properties and interactions of the selected leads with the active site of the target after successful molecular docking simulation with the pyrx virtual screening tool were also examined. The results of these study identified seven lead compounds; C49- alpha-carissanol (-7.6 kcal/mol), C13- Catechin (-7.4 kcal/mol), C45- Salicin (-7.4 kcal/mol), C6- Bisnorargemonine (-7.3 kcal/mol), C36- Pallidine (-7.1 kcal/mol), S4- Lacosamide (-7.1 kcal/mol), and S7- Acetazolamide (-6.4 kcal/mol) for CA II (3HS4 receptor). These leads compounds are probable inhibitors of this drug target due to the observed good binding affinities and favourable interactions with the active site of the drug target, excellent ADMET profiles, PASS Properties, drug-likeness, lead-likeness and oral bioavailability properties. The identified leads have better binding energies as compared to the binding energies of the two standards. Thus, seven identified lead compounds can be developed further towards the development of new anti-epileptic medications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug-likeness" title="drug-likeness">drug-likeness</a>, <a href="https://publications.waset.org/abstracts/search?q=phytochemicals" title=" phytochemicals"> phytochemicals</a>, <a href="https://publications.waset.org/abstracts/search?q=carbonic%20anhydrases" title=" carbonic anhydrases"> carbonic anhydrases</a>, <a href="https://publications.waset.org/abstracts/search?q=metalloeazymes" title=" metalloeazymes"> metalloeazymes</a>, <a href="https://publications.waset.org/abstracts/search?q=active%20site" title=" active site"> active site</a>, <a href="https://publications.waset.org/abstracts/search?q=ADMET" title=" ADMET"> ADMET</a> </p> <a href="https://publications.waset.org/abstracts/186547/screening-of-phytochemicals-compounds-from-chasmanthera-dependens-and-carissa-edulis-as-potential-inhibitors-of-carbonic-anhydrases-ca-ii-3hs4-receptor-using-a-target-based-drug-design" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186547.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">56</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1213</span> Dimethyl fumarate Alleviates Valproic Acid-Induced Autism in Wistar Rats via Activating NRF-2 and Inhibiting NF-κB Pathways</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sandy%20Elsayed">Sandy Elsayed</a>, <a href="https://publications.waset.org/abstracts/search?q=Aya%20Mohamed"> Aya Mohamed</a>, <a href="https://publications.waset.org/abstracts/search?q=Noha%20Nassar"> Noha Nassar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social deficits and repetitive behavior. Multiple studies suggest that oxidative stress and neuroinflammation are key factors in the etiology of ASD and often associated with worsening of ASD-related behaviors. Nuclear factor erythroid 2-related factor 2 (NRF-2) is a transcription factor that promotes expression of antioxidant response element genes in oxidative stress. In ASD subjects, decreased expression of NRF-2 in frontal cortex shifted the redox homeostasis towards oxidative stress, and resulted in inflammation evidenced by elevation of nuclear factor kappa B (NF-κB) transcriptional activity. Dimethyl fumarate (DMF) is a NRF-2 activator that is used in the treatment of psoriasis and multiple sclerosis. It participates in the transcriptional control of inflammatory factors via inhibition of NF-κB and its downstream targets. This study aimed to investigate the role of DMF in alleviating the cognitive impairments and behavior deficits associated with ASD through mitigation of oxidative stress and inflammation in prenatal valproic acid (VPA) rat model of autism. Methods: Pregnant female Wistar rats received a single intraperitoneal injection of VPA (600 mg/kg) to induce autistic-like-behavioral and neurobiological alterations in their offspring. Chronic oral gavage of DMF (150mg/kg/day) started from postnatal day (PND) 24 till PND62 (39 days). Prenatal VPA exposure elicited autistic behaviors including decreased social interaction and stereotyped behavior. Social interaction was evaluated using three-chamber sociability test and calculation of sociability index (SI), while stereotyped repetitive behavior and anxiety associated with ASD were assessed using marble burying test (MBT). Biochemical analyses were done on prefrontal cortex homogenates including NRF-2, and NF-κB expression. Moreover, inducible nitric oxide synthase (iNOS) gene expression and tumor necrosis factor (TNF-) protein expression were evaluated as markers of inflammation. Results: Prenatal VPA elicited decreased social interaction shown by decreased SI compared to control group (p < 0.001) and DMF enhanced SI (p < 0.05). In MBT, prenatal injection of VPA manifested stereotyped behavior and enhanced number of buried marbles compared to control (p < 0.05) and DMF reduced the anxiety-related behavior in rats exhibiting ASD-like behaviors (p < 0.05). In prefrontal cortex, NRF-2 expression was downregulated in prenatal VPA model (p < 0.0001) and DMF reversed this effect (p < 0.0001). The inflammatory transcription factor NF-κB was elevated in prenatal VPA model (p < 0.0001) and reduced (p < 0.0001) upon NRF-2 activation by DMF. Prenatal VPA expressed higher levels of proinflammatory cytokine TNF- compared to control group (p < 0.0001) and DMF reduced it (p < 0.0001). Finally, the gene expression of iNOS was downregulated upon NRF-2 activation by DMF (p < 0.01). Conclusion: This study proposes that DMF is a potential agent that can be used to ameliorate autistic-like-changes through NRF-2 activation along with NF-κB downregulation and therefore, it is a promising novel therapy for ASD. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=autism%20spectrum%20disorders" title="autism spectrum disorders">autism spectrum disorders</a>, <a href="https://publications.waset.org/abstracts/search?q=dimethyl%20fumarate" title=" dimethyl fumarate"> dimethyl fumarate</a>, <a href="https://publications.waset.org/abstracts/search?q=neuroinflammation" title=" neuroinflammation"> neuroinflammation</a>, <a href="https://publications.waset.org/abstracts/search?q=NRF-2" title=" NRF-2"> NRF-2</a> </p> <a href="https://publications.waset.org/abstracts/186497/dimethyl-fumarate-alleviates-valproic-acid-induced-autism-in-wistar-rats-via-activating-nrf-2-and-inhibiting-nf-kb-pathways" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186497.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">41</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1212</span> Azaridachta Indica (Neem) Seed Oil Effect in Experimental Arthritis – Biochemical Parameters Assessment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sasan%20Khademnematolahi">Sasan Khademnematolahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Kevine%20Kamga%20Silihe"> Kevine Kamga Silihe</a>, <a href="https://publications.waset.org/abstracts/search?q=Katar%C3%ADna%20Pru%C5%BEinsk%C3%A1"> Katarína Pružinská</a>, <a href="https://publications.waset.org/abstracts/search?q=Martina%20Chrastina"> Martina Chrastina</a>, <a href="https://publications.waset.org/abstracts/search?q=Elisabeth%20Louise%20Ndjengue%20Mindang"> Elisabeth Louise Ndjengue Mindang</a>, <a href="https://publications.waset.org/abstracts/search?q=Franti%C5%A1ek%20Dr%C3%A1fi"> František Dráfi</a>, <a href="https://publications.waset.org/abstracts/search?q=Katar%C3%ADna%20Bauerov%C3%A1"> Katarína Bauerová</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: In ethnomedicine, plant parts and compounds are traditionally utilized to treat many disorders. Azadirachta indica, known as Neem, has been traditionally used in medicinal practices. Neem has various pharmaceutical activities, such as antioxidant and anti-inflammatory, due to the content of bioactive compounds like nimbolide, azadirachtin, and gedunin.Through its effect on pathological inflammatory processes, supplementation with it could alleviate the symptoms of rheumatoid arthritis (RA). Methods: This research aimed to assess Neem seed oil's impact on rats with adjuvant arthritis. Three doses in monotherapy and two in combination with methotrexate (MTX) have been studied and their effect was compared. Neem p.o. doses of 100, 200, and 300 mg/kg and MTX p.o. doses of 0.3 mg/kg were examined. After clinical parameters assessment, biochemical analysis was performed in plasma. Results: During the acute phase of the experimental arthritis (Day21), levels of MMP-9, MCP-1 and cytokines IL-1beta and IL-17A were measured. The positive results of inflammatory mediators evaluation in plasma encourage additional analysis also in related tissues to prove if Neem seed oil can be used as an adjuvant therapy for RA. Conclusion: In this study, the combination therapy of Neem with MTX was most effective from all therapies investigated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adjuvant" title="adjuvant">adjuvant</a>, <a href="https://publications.waset.org/abstracts/search?q=neem" title=" neem"> neem</a>, <a href="https://publications.waset.org/abstracts/search?q=methotrexate" title=" methotrexate"> methotrexate</a>, <a href="https://publications.waset.org/abstracts/search?q=arthritis" title=" arthritis"> arthritis</a> </p> <a href="https://publications.waset.org/abstracts/186176/azaridachta-indica-neem-seed-oil-effect-in-experimental-arthritis-biochemical-parameters-assessment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186176.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">46</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1211</span> Cytotoxicity and Androgenic Potential of Antifungal Drug Substances on MDA-KB2 Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Benchouala%20Amira">Benchouala Amira</a>, <a href="https://publications.waset.org/abstracts/search?q=Bojic%20Clement"> Bojic Clement</a>, <a href="https://publications.waset.org/abstracts/search?q=Poupin%20Pascal"> Poupin Pascal</a>, <a href="https://publications.waset.org/abstracts/search?q=Cossu%20Leguille-carole"> Cossu Leguille-carole</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of this study is to evaluate in vitro the cytotoxic and androgenic potential of several antifungal molecules (amphotericin B, econazole, ketoconazole and miconazole) on MDA-Kb2 cell lines. This biological model is an effective tool for the detection of endocrine disruptors because it responds well to the main agonist of the androgen receptor (testosterone) and also to an antagonist: flutamide. The cytotoxicity of each chemical compound tested was measured using an MTT assay (tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) which measures the activity of the reductase function of mitochondrial succinate dehydrogenase enzymes of cultured cells. This complementary cytotoxicity test is essential to ensure that the effects of reduction in luminescence intensity observed during androgenic tests are only attributable to the anti-androgenic action of the compounds tested and not to their possible cytotoxic properties. Tests of the androgenic activity of antifungals show that these compounds do not have the capacity to induce transcription of the luciferase gene. These compounds do not exert an androgenic effect on MDA-Kb2 cells in culture for the environmental concentrations tested. The addition of flutamide for the same tested concentrations of antifungal molecules reduces the luminescence induced by amphotericin B, econazole and miconazole, which is explained by a strong interaction of these molecules with flutamide which may have a greater toxic effect than when tested alone. The cytotoxicity test shows that econazole and ketoconazole can cause cell death at certain concentrations tested. This cell mortality is perhaps induced by a direct or indirect action on deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or proteins necessary for cell division. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title="cytotoxicity">cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=androgenic%20potential" title=" androgenic potential"> androgenic potential</a>, <a href="https://publications.waset.org/abstracts/search?q=antifungals" title=" antifungals"> antifungals</a>, <a href="https://publications.waset.org/abstracts/search?q=MDA-Kb2" title=" MDA-Kb2"> MDA-Kb2</a> </p> <a href="https://publications.waset.org/abstracts/186088/cytotoxicity-and-androgenic-potential-of-antifungal-drug-substances-on-mda-kb2-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186088.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">48</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1210</span> Azaridachta indica (Neem) Seed Oil Effect in Experimental Arthritis: Biochemical Parameters Assessment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sasan%20Khademnematolahi">Sasan Khademnematolahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Kevine%20Kamga%20Silihe"> Kevine Kamga Silihe</a>, <a href="https://publications.waset.org/abstracts/search?q=Katar%C3%ADna%20Pru%C5%BEinsk%C3%A1"> Katarína Pružinská</a>, <a href="https://publications.waset.org/abstracts/search?q=Martina%20Chrastina"> Martina Chrastina</a>, <a href="https://publications.waset.org/abstracts/search?q=Elisabeth%20Louise%20Ndjengue%20Mindang"> Elisabeth Louise Ndjengue Mindang</a>, <a href="https://publications.waset.org/abstracts/search?q=Franti%C5%A1ek%20Dr%C3%A1fi"> František Dráfi</a>, <a href="https://publications.waset.org/abstracts/search?q=Katar%C3%ADna%20Bauerov%C3%A1"> Katarína Bauerová</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: In ethnomedicine, plant parts and compounds are traditionally utilized to treat many disorders. Azadirachta indica, known as Neem, has been traditionally used in medicinal practices. Due to the presence of bioactive substances such as nimbolide, azadirachtin, and gedunin, Neem offers a variety of medicinal properties, including anti-inflammatory and antioxidant properties. Through its effect on pathological inflammatory processes, supplementation with it could alleviate the symptoms of rheumatoid arthritis (RA). Methods: This research aimed to assess Neem seed oil's impact on rats with adjuvant arthritis. Three doses in monotherapy and two in combination with methotrexate (MTX) have been studied, and their effect was compared. Neem p.o. doses of 100, 200, and 300 mg/kg and MTX p.o. doses of 0.3 mg/kg were examined. After clinical parameters assessment, biochemical analysis was performed in plasma. Results: During the acute phase of the experimental arthritis (Day21), levels of MMP-9, MCP-1, and cytokines IL-1beta and IL-17A were measured. The positive results of inflammatory mediators evaluation in plasma encourage additional analysis also in related tissues to prove if Neem seed oil can be used as an adjuvant therapy for RA. Conclusion: In this study, the combination therapy of Neem with MTX was the most effective of all therapies investigated. Acknowledgement: SAIA PROJECT of Kevine Kamga Silihe, Slovakia-Cameroon 2023: “The effect of Crocus sativus L (Saffron), Azadirachta indica (Neem) and their main bioactives compounds in combinatory treatment with methotrexate on experimental arthritis”, VEGA 2/0079/24, VEGA 2/0136/20, VEGA 2/0126/23 and VEGA 2/0091/23. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adjuvant" title="adjuvant">adjuvant</a>, <a href="https://publications.waset.org/abstracts/search?q=Neem" title=" Neem"> Neem</a>, <a href="https://publications.waset.org/abstracts/search?q=methotrexate" title=" methotrexate"> methotrexate</a>, <a href="https://publications.waset.org/abstracts/search?q=arthritis" title=" arthritis"> arthritis</a> </p> <a href="https://publications.waset.org/abstracts/186053/azaridachta-indica-neem-seed-oil-effect-in-experimental-arthritis-biochemical-parameters-assessment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186053.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">44</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1209</span> Design, Molecular Modeling, Synthesize, and Biological Evaluation of Some Dual Inhibitors of Soluble Epoxide Hydrolase (sEH) and Cyclooxygenase 2 (COX-2)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elham%20Rezaee">Elham Rezaee</a>, <a href="https://publications.waset.org/abstracts/search?q=Sayyed%20Abbas%20Tabatabai"> Sayyed Abbas Tabatabai</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dual inhibition of COX-2 and sEH enzymes represents one of the distinct pharmaceutical approaches for the treatment of inflammation, pain, cancers, and other diseases. The discovery of these inhibitors for treatment is a great deal of attention because of some advantages such as increased efficacy, a promising safety profile, ease of formulation, and better target engagement. In this research, based on the structure-activity relationship of COX-2 and sEH inhibitors, some amide derivatives with oxadiazole and dihydropyrimidinone rings against sEH and COX-2 enzymes were developed. The designed compounds showed high affinity to the active site of both enzymes in docking studies and were synthesized in good yield and characterized by IR, Mass, 1HNMR, and 13CNMR. All of the novel compounds exhibited considerable in-vitro sEH and COX-2 inhibitory activities in comparison with 12-(3-Adamantan-1-yl-ureido)- dodecanoic acid and celecoxib (a potent urea-based sEH inhibitor and selective nonsteroidal anti-inflammatory drug, respectively). Ethyl 6-methyl-4-(4-(4-(methylsulfonyl)benzamido)phenyl)-2-oxo-1,2,3,4-tetrahydropyrimidine-5-carboxylate was found to be the most selective COX-2 inhibitor (COX-2/COX-1 ratio: 683) with IC50 value of 2.1 nM targeting sEH enzyme. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=COX-2" title="COX-2">COX-2</a>, <a href="https://publications.waset.org/abstracts/search?q=dual%20inhibitors" title=" dual inhibitors"> dual inhibitors</a>, <a href="https://publications.waset.org/abstracts/search?q=sEH" title=" sEH"> sEH</a>, <a href="https://publications.waset.org/abstracts/search?q=synthesis" title=" synthesis"> synthesis</a> </p> <a href="https://publications.waset.org/abstracts/186048/design-molecular-modeling-synthesize-and-biological-evaluation-of-some-dual-inhibitors-of-soluble-epoxide-hydrolase-seh-and-cyclooxygenase-2-cox-2" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186048.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">50</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1208</span> Bioactivity Profiling of Botswana’s Medicinal Ethnobotany With Potential to Mitigate Oxidative Stress</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Motlhanka">Daniel Motlhanka</a>, <a href="https://publications.waset.org/abstracts/search?q=Neo%20Kerebotswe"> Neo Kerebotswe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The strong and long history of use of medicinal plants in Botswana to address existing and emerging health threats provides undebatable evidence for their potential as innovative therapeutic tools. The prevalence of emerging health threats, such as COVID-19 and hard-to-treat non-communicable diseases, warrants the scientific community to revisit and exploit ethnopharmacology for its potential as a source of therapeutic tools. Many studies conducted on bioactivity-guided bioassays of ethnobotanical resources have proved a number of health beneficial properties of these plants, such as free radical scavenging, anti-inflammatory, antimicrobial and, most importantly, the capability of medicinal plants to alleviate oxidative stress. In this work, a number of medicinal plants used in Botswana traditional medicine were investigated for both their free radical scavenging capability and total phenolic contents using the Free Radical Scavenging Power (FRSP) and Folin Ciocalteau (FC) method. At 100 micrograms/ml all the studied plants expressed above 90% Scavenging power and expressed total phenolic contents between 5000- 8890 mg/L.GAE. These plants are promising tools for engineering active therapeutic tools against life-threatening diseases of oxidative stress origin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title="oxidative stress">oxidative stress</a>, <a href="https://publications.waset.org/abstracts/search?q=non-communicable%20diseases" title=" non-communicable diseases"> non-communicable diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20phenolics" title=" total phenolics"> total phenolics</a>, <a href="https://publications.waset.org/abstracts/search?q=ethnobotanicals" title=" ethnobotanicals"> ethnobotanicals</a> </p> <a href="https://publications.waset.org/abstracts/186011/bioactivity-profiling-of-botswanas-medicinal-ethnobotany-with-potential-to-mitigate-oxidative-stress" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186011.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">49</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1207</span> Liquid Biopsy and Screening Biomarkers in Glioma Grading</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20Abdu%20Qaseem%20Shamsan">Abdullah Abdu Qaseem Shamsan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Gliomas represent the most frequent, heterogeneous group of tumors arising from glial cells, characterized by difficult monitoring, poor prognosis, and fatality. Tissue biopsy is an established procedure for tumor cell sampling that aids diagnosis, tumor grading, and prediction of prognosis. We studied and compared the levels of liquid biopsy markers in patients with different grades of glioma. Also, it tried to establish the potential association between glioma and specific blood groups antigen. Result: 78 patients were identified, among whom maximum percentage with glioblastoma possessed blood group O+ (53.8%). The second highest frequency had blood group A+ (20.4%), followed by B+ (9.0%) and A- (5.1%), and least with O-. Liquid biopsy biomarkers comprised of ALT, LDH, lymphocytes, Urea, Alkaline phosphatase, AST Neutrophils, and CRP. The levels of all the components increased significantly with the severity of glioma, with maximum levels seen in glioblastoma (grade IV), followed by grade III and grade II respectively. Conclusion: Gliomas possess significant clinical challenges due to their progression with heterogeneous nature and aggressive behavior. Liquid biopsy is a non-invasive approach which aids to establish the status of the patient and determine the tumor grade, therefore may show diagnostic and prognostic utility. Additionally, our study provides evidence to demonstrate the role of ABO blood group antigens in the development of glioma. However, future clinical research on liquid biopsy will improve the sensitivity and specificity of these tests and validate their clinical usefulness to guide treatment approaches. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=GBM%3A%20glioblastoma%20multiforme" title="GBM: glioblastoma multiforme">GBM: glioblastoma multiforme</a>, <a href="https://publications.waset.org/abstracts/search?q=CT%3A%20computed%20tomography" title=" CT: computed tomography"> CT: computed tomography</a>, <a href="https://publications.waset.org/abstracts/search?q=MRI%3A%20magnetic%20resonance%20imaging" title=" MRI: magnetic resonance imaging"> MRI: magnetic resonance imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=ctRNA%3A%20circulating%20tumor%20RNA" title=" ctRNA: circulating tumor RNA"> ctRNA: circulating tumor RNA</a> </p> <a href="https://publications.waset.org/abstracts/185991/liquid-biopsy-and-screening-biomarkers-in-glioma-grading" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185991.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">51</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/pharmacological-and-pharmaceutical-sciences?page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/pharmacological-and-pharmaceutical-sciences?page=3">3</a></li> <li class="page-item"><a class="page-link" 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