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-webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">DNA</h1><div class="u-tcGrayDark">222 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>DNA</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/DNA">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/DNA/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/DNA/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/DNA/MostRecent">Newest Papers</a></li><li><a class="" href="https://www.academia.edu/People/DNA">People</a></li></ul></div><style type="text/css">ul.nav{flex-direction:row}@media(max-width: 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u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/58007549/Australian_spiny_mountain_crayfish_and_their_temnocephalan_ectosymbionts_an_ancient_association_on_the_edge_of_coextinction">Australian spiny mountain crayfish and their temnocephalan ectosymbionts: an ancient association on the edge of coextinction?</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Australian spiny mountain crayfish (Euastacus, Parastacidae) and their ecotosymbiotic temnocephalan flatworms (Temnocephalida, Platyhelminthes) may have co-occurred and interacted through deep time, during a period of major environmental... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_58007549" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Australian spiny mountain crayfish (Euastacus, Parastacidae) and their ecotosymbiotic temnocephalan flatworms (Temnocephalida, Platyhelminthes) may have co-occurred and interacted through deep time, during a period of major environmental change. Therefore, reconstructing the history of their association is of evolutionary, ecological, and conservation significance. Here, time-calibrated Bayesian phylogenies of Euastacus species and their temnocephalans (Temnohaswellia and Temnosewellia) indicate near-synchronous diversifications from the Cretaceous. Statistically significant cophylogeny correlations between associated clades suggest linked evolutionary histories. However, there is a stronger signal of codivergence and greater host specificity in Temnosewellia, which co-occurs with Euastacus across its range. Phylogeography and analyses of evolutionary distinctiveness (ED) suggest that regional differences in the impact of climate warming and drying had major effects both on crayfish...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58007549" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d738089b733c93e3be0f0f437679b24c" rel="nofollow" data-download="{"attachment_id":72631713,"asset_id":58007549,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72631713/download_file?st=MTczMjcyODk4Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="81741437" href="https://independent.academia.edu/LesterCannon">Lester Cannon</a><script data-card-contents-for-user="81741437" type="text/json">{"id":81741437,"first_name":"Lester","last_name":"Cannon","domain_name":"independent","page_name":"LesterCannon","display_name":"Lester Cannon","profile_url":"https://independent.academia.edu/LesterCannon?f_ri=48057","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_58007549 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58007549"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58007549, container: ".js-paper-rank-work_58007549", }); 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Therefore, reconstructing the history of their association is of evolutionary, ecological, and conservation significance. Here, time-calibrated Bayesian phylogenies of Euastacus species and their temnocephalans (Temnohaswellia and Temnosewellia) indicate near-synchronous diversifications from the Cretaceous. Statistically significant cophylogeny correlations between associated clades suggest linked evolutionary histories. However, there is a stronger signal of codivergence and greater host specificity in Temnosewellia, which co-occurs with Euastacus across its range. Phylogeography and analyses of evolutionary distinctiveness (ED) suggest that regional differences in the impact of climate warming and drying had major effects both on crayfish...","downloadable_attachments":[{"id":72631713,"asset_id":58007549,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":81741437,"first_name":"Lester","last_name":"Cannon","domain_name":"independent","page_name":"LesterCannon","display_name":"Lester Cannon","profile_url":"https://independent.academia.edu/LesterCannon?f_ri=48057","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2467,"name":"Conservation 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href="https://www.academia.edu/15432569/New_understanding_of_the_molecular_mechanism_of_receptor_mediated_genomic_actions_of_the_vitamin_D_hormone">New understanding of the molecular mechanism of receptor-mediated genomic actions of the vitamin D hormone</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15432569" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f9ee5e32fed5a5eec0c4d50abab3ee2d" rel="nofollow" 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data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A autora apresenta no primeiro capitulo do livro, casos reais de dilemas éticos que envolvem não somente a relação medico-paciente, mas, também todas as implicações futuras nas relações familiares, ao revelar que determinado membro da família não é de fato filho biológico, e que naquela família existe um segredo que se revelado pode ter consequências inimagináveis. 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href="https://www.academia.edu/78262729/Human_Fallopian_Tube_Mesenchymal_Stromal_Cells_Enhance_Bone_Regeneration_in_a_Xenotransplanted_Model">Human Fallopian Tube Mesenchymal Stromal Cells Enhance Bone Regeneration in a Xenotransplanted Model</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/78262729" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c1d117394b1cea3d59da5f8f79a2f701" rel="nofollow" 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href="https://www.academia.edu/Documents/in/Viscoelasticity">Viscoelasticity</a>, <script data-card-contents-for-ri="2383" type="text/json">{"id":2383,"name":"Viscoelasticity","url":"https://www.academia.edu/Documents/in/Viscoelasticity?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22050" href="https://www.academia.edu/Documents/in/Cytotoxicity">Cytotoxicity</a><script data-card-contents-for-ri="22050" type="text/json">{"id":22050,"name":"Cytotoxicity","url":"https://www.academia.edu/Documents/in/Cytotoxicity?f_ri=48057","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=77240402]'), work: {"id":77240402,"title":"Synthesis of Guanidinium-Modified Hyaluronic Acid 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js-work-link" href="https://www.academia.edu/71515961/A_Population_Based_Ant_Colony_Optimization_Approach_for_DNA_Sequence_Optimization">A Population-Based Ant Colony Optimization Approach for DNA Sequence Optimization</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">DNA computing is a new computing paradigm which uses bio-molecular as information storage media and biochemical tools as information processing operators. It has shows many successful and promising results for various applications. Since... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_71515961" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">DNA computing is a new computing paradigm which uses bio-molecular as information storage media and biochemical tools as information processing operators. It has shows many successful and promising results for various applications. Since DNA reactions are probabilistic reactions, it can cause the different results for the same situations, which can be regarded as errors in the computation. To overcome the drawbacks, much works have focused to design the error-minimized DNA sequences to improve the reliability of DNA computing. In this research, Population-based ACO (P-ACO) is proposed to solve the DNA sequence optimization. P-ACO approach is a meta-heuristic algorithm that uses some ants to obtain the solutions based on the pheromone in their colony. The DNA sequence design problem is modelled by four nodes, representing four DNA bases (A, T, C, and G). The results from the proposed algorithm are compared with other sequence design methods, which are Genetic Algorithm (GA), and Mult...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/71515961" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="101277564" href="https://independent.academia.edu/AlexTrikurnia">Tri Kurniawan</a><script data-card-contents-for-user="101277564" type="text/json">{"id":101277564,"first_name":"Tri","last_name":"Kurniawan","domain_name":"independent","page_name":"AlexTrikurnia","display_name":"Tri Kurniawan","profile_url":"https://independent.academia.edu/AlexTrikurnia?f_ri=48057","photo":"https://0.academia-photos.com/101277564/22167064/21398746/s65_alex.trikurnia.jpg"}</script></span></span></li><li class="js-paper-rank-work_71515961 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="71515961"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 71515961, container: ".js-paper-rank-work_71515961", }); 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class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66333822/Human_evolution_writ_small">Human evolution writ small</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66333822" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ed50270c37984a7dbb67442a09581f7b" rel="nofollow" data-download="{"attachment_id":77568147,"asset_id":66333822,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button 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class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/61069355/Nucleic_acid_quantity_and_quality_from_paraffin_blocks_Defining_optimal_fixation_processing_and_DNA_RNA_extraction_techniques">Nucleic acid quantity and quality from paraffin blocks: Defining optimal fixation, processing and DNA/RNA extraction techniques</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/61069355" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cabdf350c28a9382b11297ff319bef3d" rel="nofollow" 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type="text/json">{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="51791" href="https://www.academia.edu/Documents/in/PCR">PCR</a>, <script data-card-contents-for-ri="51791" type="text/json">{"id":51791,"name":"PCR","url":"https://www.academia.edu/Documents/in/PCR?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71260" href="https://www.academia.edu/Documents/in/Molecular_plant_pathology">Molecular plant pathology</a><script data-card-contents-for-ri="71260" type="text/json">{"id":71260,"name":"Molecular plant pathology","url":"https://www.academia.edu/Documents/in/Molecular_plant_pathology?f_ri=48057","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=61069355]'), work: {"id":61069355,"title":"Nucleic acid quantity and quality from 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Turashvili","profile_url":"https://independent.academia.edu/GulisaTurashvili?f_ri=48057","photo":"https://0.academia-photos.com/32389335/128035749/117424973/s65_gulisa.turashvili.jpeg"}],"research_interests":[{"id":3701,"name":"RNA","url":"https://www.academia.edu/Documents/in/RNA?f_ri=48057","nofollow":false},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057","nofollow":false},{"id":51791,"name":"PCR","url":"https://www.academia.edu/Documents/in/PCR?f_ri=48057","nofollow":false},{"id":71260,"name":"Molecular plant pathology","url":"https://www.academia.edu/Documents/in/Molecular_plant_pathology?f_ri=48057","nofollow":false},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=48057"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=48057"},{"id":244814,"name":"Clinical 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reaction","url":"https://www.academia.edu/Documents/in/reverse_transcriptase_polymerase_chain_reaction?f_ri=48057"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_58486871" data-work_id="58486871" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/58486871/Designing_a_Bio_responsive_Robot_from_DNA_Origami">Designing a Bio-responsive Robot from DNA Origami</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58486871" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i 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itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43564668" href="https://independent.academia.edu/EldadBenIshay">Eldad Ben Ishay</a><script data-card-contents-for-user="43564668" type="text/json">{"id":43564668,"first_name":"Eldad Ben","last_name":"Ishay","domain_name":"independent","page_name":"EldadBenIshay","display_name":"Eldad Ben Ishay","profile_url":"https://independent.academia.edu/EldadBenIshay?f_ri=48057","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_58486871 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58486871"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58486871, container: ".js-paper-rank-work_58486871", }); });</script></li><li class="js-percentile-work_58486871 InlineList-item InlineList-item--bordered 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Ishay","profile_url":"https://independent.academia.edu/EldadBenIshay?f_ri=48057","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":68,"name":"Bioengineering","url":"https://www.academia.edu/Documents/in/Bioengineering?f_ri=48057","nofollow":false},{"id":77,"name":"Robotics","url":"https://www.academia.edu/Documents/in/Robotics?f_ri=48057","nofollow":false},{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=48057","nofollow":false},{"id":1131,"name":"Biomedical Engineering","url":"https://www.academia.edu/Documents/in/Biomedical_Engineering?f_ri=48057","nofollow":false},{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology?f_ri=48057"},{"id":3216,"name":"Genomics","url":"https://www.academia.edu/Documents/in/Genomics?f_ri=48057"},{"id":11972,"name":"Nanomedicine","url":"https://www.academia.edu/Documents/in/Nanomedicine?f_ri=48057"},{"id":17733,"name":"Nanotechnology","url":"https://www.academia.edu/Documents/in/Nanotechnology?f_ri=48057"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=48057"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057"},{"id":49685,"name":"CAD","url":"https://www.academia.edu/Documents/in/CAD?f_ri=48057"},{"id":58316,"name":"Sequencing","url":"https://www.academia.edu/Documents/in/Sequencing?f_ri=48057"},{"id":178906,"name":"Nucleic Acids","url":"https://www.academia.edu/Documents/in/Nucleic_Acids?f_ri=48057"},{"id":250811,"name":"DNA origami.","url":"https://www.academia.edu/Documents/in/DNA_origami?f_ri=48057"},{"id":1362652,"name":"Nucleic Acid Conformation","url":"https://www.academia.edu/Documents/in/Nucleic_Acid_Conformation?f_ri=48057"},{"id":1649745,"name":"Oligonucleotides","url":"https://www.academia.edu/Documents/in/Oligonucleotides?f_ri=48057"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_57593960 coauthored" data-work_id="57593960" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/57593960/Cell_cycle_parameters_inAedes_albopictus_mosquito_cells">Cell cycle parameters inAedes albopictus mosquito cells</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We have analyzed cell cycle parameters for the Aedes albopictus C7-10 mosquito cell line, which has been systematically developed for somatic cell genetics, expression of transfected genes, and synthesis of hormone-inducible proteins. In... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_57593960" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We have analyzed cell cycle parameters for the Aedes albopictus C7-10 mosquito cell line, which has been systematically developed for somatic cell genetics, expression of transfected genes, and synthesis of hormone-inducible proteins. In rapidly cycling cells, we measured a generation time of 10-12 h. The duration of mitosis (M) was &amp;lt; or = 1 h, and the DNA synthesis phase (S) required 6 h. Unlike Drosophila melanogaster Kc cells, in which the G2 gap is substantially longer than G1, in C7-10 cells G1 and G2 each lasted approximately 2 h. In these cells, the duration of both S and G2 was independent of the population doubling time, and the increase in population doubling time as cells approached confluency was due to prolongation of G1. When treated with the insect steroid hormone, 20-hydroxyecdysone, C7-10 mosquito cells complete the cycle in progress before undergoing a reversible arrest.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/57593960" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="215824721" href="https://spanalumni.academia.edu/AnnFallon">Ann Fallon</a><script data-card-contents-for-user="215824721" type="text/json">{"id":215824721,"first_name":"Ann","last_name":"Fallon","domain_name":"spanalumni","page_name":"AnnFallon","display_name":"Ann Fallon","profile_url":"https://spanalumni.academia.edu/AnnFallon?f_ri=48057","photo":"https://0.academia-photos.com/215824721/74332423/62827166/s65_ann.fallon.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-57593960">+1</span><div class="hidden js-additional-users-57593960"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/AnnaGerenday">Anna Gerenday</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-57593960'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-57593960').html(); 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container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_57593960 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="57593960"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 57593960; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=57593960]").text(description); $(".js-view-count-work_57593960").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_57593960").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="57593960"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="156" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="173" href="https://www.academia.edu/Documents/in/Zoology">Zoology</a>, <script data-card-contents-for-ri="173" type="text/json">{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6599" href="https://www.academia.edu/Documents/in/Flow_Cytometry">Flow Cytometry</a><script data-card-contents-for-ri="6599" type="text/json">{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=48057","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=57593960]'), work: {"id":57593960,"title":"Cell cycle parameters inAedes albopictus mosquito cells","created_at":"2021-10-13T11:56:21.464-07:00","url":"https://www.academia.edu/57593960/Cell_cycle_parameters_inAedes_albopictus_mosquito_cells?f_ri=48057","dom_id":"work_57593960","summary":"We have analyzed cell cycle parameters for the Aedes albopictus C7-10 mosquito cell line, which has been systematically developed for somatic cell genetics, expression of transfected genes, and synthesis of hormone-inducible proteins. In rapidly cycling cells, we measured a generation time of 10-12 h. The duration of mitosis (M) was \u0026amp;lt; or = 1 h, and the DNA synthesis phase (S) required 6 h. Unlike Drosophila melanogaster Kc cells, in which the G2 gap is substantially longer than G1, in C7-10 cells G1 and G2 each lasted approximately 2 h. In these cells, the duration of both S and G2 was independent of the population doubling time, and the increase in population doubling time as cells approached confluency was due to prolongation of G1. When treated with the insect steroid hormone, 20-hydroxyecdysone, C7-10 mosquito cells complete the cycle in progress before undergoing a reversible arrest.","downloadable_attachments":[],"ordered_authors":[{"id":215824721,"first_name":"Ann","last_name":"Fallon","domain_name":"spanalumni","page_name":"AnnFallon","display_name":"Ann Fallon","profile_url":"https://spanalumni.academia.edu/AnnFallon?f_ri=48057","photo":"https://0.academia-photos.com/215824721/74332423/62827166/s65_ann.fallon.png"},{"id":48128438,"first_name":"Anna","last_name":"Gerenday","domain_name":"independent","page_name":"AnnaGerenday","display_name":"Anna Gerenday","profile_url":"https://independent.academia.edu/AnnaGerenday?f_ri=48057","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=48057","nofollow":false},{"id":173,"name":"Zoology","url":"https://www.academia.edu/Documents/in/Zoology?f_ri=48057","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=48057","nofollow":false},{"id":6599,"name":"Flow Cytometry","url":"https://www.academia.edu/Documents/in/Flow_Cytometry?f_ri=48057","nofollow":false},{"id":9113,"name":"Cell Cycle","url":"https://www.academia.edu/Documents/in/Cell_Cycle?f_ri=48057"},{"id":38650,"name":"Cell Division","url":"https://www.academia.edu/Documents/in/Cell_Division?f_ri=48057"},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=48057"},{"id":442493,"name":"Larva","url":"https://www.academia.edu/Documents/in/Larva?f_ri=48057"},{"id":1168109,"name":"Mitotic Index","url":"https://www.academia.edu/Documents/in/Mitotic_Index?f_ri=48057"},{"id":1388461,"name":"Aedes","url":"https://www.academia.edu/Documents/in/Aedes?f_ri=48057"},{"id":2194607,"name":"Somatic Cells","url":"https://www.academia.edu/Documents/in/Somatic_Cells?f_ri=48057"},{"id":2235833,"name":"Dna Synthesis","url":"https://www.academia.edu/Documents/in/Dna_Synthesis?f_ri=48057"},{"id":3134154,"name":"Doubling Time","url":"https://www.academia.edu/Documents/in/Doubling_Time?f_ri=48057"},{"id":3789883,"name":"Paediatrics and reproductive medicine","url":"https://www.academia.edu/Documents/in/Paediatrics_and_reproductive_medicine?f_ri=48057"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_54438334" data-work_id="54438334" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/54438334/The_use_of_gene_probes_in_the_rapid_analysis_of_natural_microbial_communities">The use of gene probes in the rapid analysis of natural microbial communities</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/54438334" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bee994e0341e49058e52227f1a46ba28" rel="nofollow" data-download="{"attachment_id":70801200,"asset_id":54438334,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/70801200/download_file?st=MTczMjcyODk4Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="31088772" href="https://independent.academia.edu/GarySayler">Gary Sayler</a><script 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{ var workId = 54438334; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_54438334"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_54438334 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="54438334"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 54438334; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=54438334]").text(description); $(".js-view-count-work_54438334").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_54438334").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="54438334"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="167" href="https://www.academia.edu/Documents/in/Physiology">Physiology</a>, <script data-card-contents-for-ri="167" type="text/json">{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="14907" href="https://www.academia.edu/Documents/in/Industrial_Biotechnology">Industrial Biotechnology</a>, <script data-card-contents-for-ri="14907" type="text/json">{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="20099" href="https://www.academia.edu/Documents/in/Sensitivity_Analysis">Sensitivity Analysis</a><script data-card-contents-for-ri="20099" type="text/json">{"id":20099,"name":"Sensitivity Analysis","url":"https://www.academia.edu/Documents/in/Sensitivity_Analysis?f_ri=48057","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=54438334]'), work: {"id":54438334,"title":"The use of gene probes in the rapid analysis of natural microbial communities","created_at":"2021-09-30T14:21:17.193-07:00","url":"https://www.academia.edu/54438334/The_use_of_gene_probes_in_the_rapid_analysis_of_natural_microbial_communities?f_ri=48057","dom_id":"work_54438334","summary":null,"downloadable_attachments":[{"id":70801200,"asset_id":54438334,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":31088772,"first_name":"Gary","last_name":"Sayler","domain_name":"independent","page_name":"GarySayler","display_name":"Gary Sayler","profile_url":"https://independent.academia.edu/GarySayler?f_ri=48057","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=48057","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=48057","nofollow":false},{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=48057","nofollow":false},{"id":20099,"name":"Sensitivity Analysis","url":"https://www.academia.edu/Documents/in/Sensitivity_Analysis?f_ri=48057","nofollow":false},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057"},{"id":63353,"name":"Identification","url":"https://www.academia.edu/Documents/in/Identification?f_ri=48057"},{"id":74761,"name":"Industrial microbiology","url":"https://www.academia.edu/Documents/in/Industrial_microbiology?f_ri=48057"},{"id":129385,"name":"DNA hybridization","url":"https://www.academia.edu/Documents/in/DNA_hybridization?f_ri=48057"},{"id":234147,"name":"ribosomal RNA","url":"https://www.academia.edu/Documents/in/ribosomal_RNA?f_ri=48057"},{"id":340277,"name":"Natural Environment","url":"https://www.academia.edu/Documents/in/Natural_Environment?f_ri=48057"},{"id":354035,"name":"Industrial microbiology and environmental biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_microbiology_and_environmental_biotechnology?f_ri=48057"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=48057"},{"id":1627116,"name":"Microbial community","url":"https://www.academia.edu/Documents/in/Microbial_community?f_ri=48057"},{"id":2274872,"name":"DNA sequence","url":"https://www.academia.edu/Documents/in/DNA_sequence?f_ri=48057"},{"id":2550936,"name":"Molecular Probe","url":"https://www.academia.edu/Documents/in/Molecular_Probe?f_ri=48057"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_53998388" data-work_id="53998388" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/53998388/The_effect_of_silver_ion_binding_and_pH_on_the_buoyant_density_of_DNA_and_its_use_in_fractionating_heterogeneous_DNA">The effect of silver ion binding and pH on the buoyant density of DNA and its use in fractionating heterogeneous DNA</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">1. The effect of pH on the buoyant density of the complexes of Ag+ with DNA has been studied using 3H-labeled human DNA and several bacterial DNAs to determine the conditions necessary for the maximum resolution of compositional... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_53998388" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">1. The effect of pH on the buoyant density of the complexes of Ag+ with DNA has been studied using 3H-labeled human DNA and several bacterial DNAs to determine the conditions necessary for the maximum resolution of compositional heterogeneity. In neutral CS2SO4 density gradients, Ag+ complexes with (G - C)-rich components are always denser than those with (A - T)-rich components, since (G - C)rich DNAs have a larger affinity for Ag+ than (A - T)-rich DNAs and their complexes are denser than (A - T)-rich complexes. In alkaline (pH greater than 9) CS2SO4 gradients, the buoyant density of the Ag+ - DNA complex is not a simple function of base composition. The Ag+ affinity of (A - T)-rich DNA is larger than that of (G - C)-rich DNA but the density of a (G - C)-rich complex is larger. Thus the ordering of the buoyant density changes depends on the amount of added Ag+. 2. The problem of resolving the density heterogeneity within a tracer DNA, and minor components of DNA, is explored and u...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/53998388" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4264a553e39a7e345577f3797711c45f" rel="nofollow" data-download="{"attachment_id":70574092,"asset_id":53998388,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/70574092/download_file?st=MTczMjcyODk4Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50587804" href="https://independent.academia.edu/CSchmid2">C. Schmid</a><script data-card-contents-for-user="50587804" type="text/json">{"id":50587804,"first_name":"C.","last_name":"Schmid","domain_name":"independent","page_name":"CSchmid2","display_name":"C. 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The effect of pH on the buoyant density of the complexes of Ag+ with DNA has been studied using 3H-labeled human DNA and several bacterial DNAs to determine the conditions necessary for the maximum resolution of compositional heterogeneity. In neutral CS2SO4 density gradients, Ag+ complexes with (G - C)-rich components are always denser than those with (A - T)-rich components, since (G - C)rich DNAs have a larger affinity for Ag+ than (A - T)-rich DNAs and their complexes are denser than (A - T)-rich complexes. In alkaline (pH greater than 9) CS2SO4 gradients, the buoyant density of the Ag+ - DNA complex is not a simple function of base composition. The Ag+ affinity of (A - T)-rich DNA is larger than that of (G - C)-rich DNA but the density of a (G - C)-rich complex is larger. Thus the ordering of the buoyant density changes depends on the amount of added Ag+. 2. The problem of resolving the density heterogeneity within a tracer DNA, and minor components of DNA, is explored and u...","downloadable_attachments":[{"id":70574092,"asset_id":53998388,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50587804,"first_name":"C.","last_name":"Schmid","domain_name":"independent","page_name":"CSchmid2","display_name":"C. Schmid","profile_url":"https://independent.academia.edu/CSchmid2?f_ri=48057","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":300,"name":"Mathematics","url":"https://www.academia.edu/Documents/in/Mathematics?f_ri=48057","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=48057","nofollow":false},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057","nofollow":false},{"id":80694,"name":"Silver","url":"https://www.academia.edu/Documents/in/Silver?f_ri=48057","nofollow":false},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=48057"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=48057"},{"id":695018,"name":"Molecular weight","url":"https://www.academia.edu/Documents/in/Molecular_weight?f_ri=48057"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity?f_ri=48057"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=48057"},{"id":1146746,"name":"Clostridium perfringens","url":"https://www.academia.edu/Documents/in/Clostridium_perfringens?f_ri=48057"},{"id":2898895,"name":"binding sites","url":"https://www.academia.edu/Documents/in/binding_sites?f_ri=48057"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_51292090 coauthored" data-work_id="51292090" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/51292090/The_architecture_of_the_12RSS_in_V_D_J_recombination_signal_and_synaptic_complexes">The architecture of the 12RSS in V(D)J recombination signal and synaptic complexes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">V(D)J recombination is initiated by RAG1 and RAG2, which together with HMGB1 bind to a recombination signal sequence (12RSS or 23RSS) to form the signal complex (SC) and then capture a complementary partner RSS, yielding the paired... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_51292090" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">V(D)J recombination is initiated by RAG1 and RAG2, which together with HMGB1 bind to a recombination signal sequence (12RSS or 23RSS) to form the signal complex (SC) and then capture a complementary partner RSS, yielding the paired complex (PC). Little is known regarding the structural changes that accompany the SC to PC transition or the structural features that allow RAG to distinguish its two asymmetric substrates. To address these issues, we analyzed the structure of the 12RSS in the SC and PC using fluorescence resonance energy transfer (FRET) and molecular dynamics modeling. The resulting models indicate that the 12RSS adopts a strongly bent V-shaped structure upon RAG/HMGB1 binding and reveal structural differences, particularly near the heptamer, between the 12RSS in the SC and PC. Comparison of models of the 12RSS and 23RSS in the PC reveals broadly similar shapes but a distinct number and location of DNA bends as well as a smaller central cavity for the 12RSS. These findin...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/51292090" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="27829b2de99be78501f702fe65a2f45b" rel="nofollow" data-download="{"attachment_id":69075525,"asset_id":51292090,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/69075525/download_file?st=MTczMjcyODk4Nyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="94261517" href="https://independent.academia.edu/CiubotaruMihai">Mihai Ciubotaru</a><script data-card-contents-for-user="94261517" type="text/json">{"id":94261517,"first_name":"Mihai","last_name":"Ciubotaru","domain_name":"independent","page_name":"CiubotaruMihai","display_name":"Mihai Ciubotaru","profile_url":"https://independent.academia.edu/CiubotaruMihai?f_ri=48057","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-51292090">+1</span><div class="hidden js-additional-users-51292090"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/DavidSchatz2">David Schatz</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-51292090'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-51292090').html(); 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Little is known regarding the structural changes that accompany the SC to PC transition or the structural features that allow RAG to distinguish its two asymmetric substrates. To address these issues, we analyzed the structure of the 12RSS in the SC and PC using fluorescence resonance energy transfer (FRET) and molecular dynamics modeling. The resulting models indicate that the 12RSS adopts a strongly bent V-shaped structure upon RAG/HMGB1 binding and reveal structural differences, particularly near the heptamer, between the 12RSS in the SC and PC. Comparison of models of the 12RSS and 23RSS in the PC reveals broadly similar shapes but a distinct number and location of DNA bends as well as a smaller central cavity for the 12RSS. These findin...","downloadable_attachments":[{"id":69075525,"asset_id":51292090,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":94261517,"first_name":"Mihai","last_name":"Ciubotaru","domain_name":"independent","page_name":"CiubotaruMihai","display_name":"Mihai Ciubotaru","profile_url":"https://independent.academia.edu/CiubotaruMihai?f_ri=48057","photo":"/images/s65_no_pic.png"},{"id":211687325,"first_name":"David","last_name":"Schatz","domain_name":"independent","page_name":"DavidSchatz2","display_name":"David Schatz","profile_url":"https://independent.academia.edu/DavidSchatz2?f_ri=48057","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=48057","nofollow":false},{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=48057","nofollow":false},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=48057","nofollow":false},{"id":178906,"name":"Nucleic Acids","url":"https://www.academia.edu/Documents/in/Nucleic_Acids?f_ri=48057","nofollow":false},{"id":598644,"name":"DNA Cleavage","url":"https://www.academia.edu/Documents/in/DNA_Cleavage?f_ri=48057"},{"id":1362652,"name":"Nucleic Acid Conformation","url":"https://www.academia.edu/Documents/in/Nucleic_Acid_Conformation?f_ri=48057"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_47846137" data-work_id="47846137" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/47846137/Sequence_specific_DNA_recognition_by_the_thyroid_transcription_factor_1_homeodomain">Sequence-specific DNA recognition by the thyroid transcription factor-1 homeodomain</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/47846137" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fb60ba3b7aabbb478da02d3d137fc1a2" rel="nofollow" data-download="{"attachment_id":66759740,"asset_id":47846137,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" 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Complete nucleotide sequence of an infectious clone of a mild isolate of tomato yellow leaf curl ... The nucleotide sequence of a field isolate of a TYLCV-like virus from Israel ... pBI121 was propagated in E. coli HB101 and shuttled... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19938800" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">... Complete nucleotide sequence of an infectious clone of a mild isolate of tomato yellow leaf curl ... The nucleotide sequence of a field isolate of a TYLCV-like virus from Israel ... pBI121 was propagated in E. coli HB101 and shuttled into Agrobacterium tumefaciens LAB4404 (Clontech ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19938800" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="58d01d143398b4548c7dc1ff023c3807" rel="nofollow" data-download="{"attachment_id":42000925,"asset_id":19938800,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42000925/download_file?st=MTczMjcyODk4OCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="40798932" href="https://kaust.academia.edu/TakashiGojobori">Takashi Gojobori</a><script data-card-contents-for-user="40798932" type="text/json">{"id":40798932,"first_name":"Takashi","last_name":"Gojobori","domain_name":"kaust","page_name":"TakashiGojobori","display_name":"Takashi Gojobori","profile_url":"https://kaust.academia.edu/TakashiGojobori?f_ri=48057","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19938800 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19938800"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19938800, container: ".js-paper-rank-work_19938800", }); 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type="text/json">{"id":7606,"name":"Medical and Veterinary Entomology","url":"https://www.academia.edu/Documents/in/Medical_and_Veterinary_Entomology?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="43257" href="https://www.academia.edu/Documents/in/Aedes_aegypti">Aedes aegypti</a>, <script data-card-contents-for-ri="43257" type="text/json">{"id":43257,"name":"Aedes aegypti","url":"https://www.academia.edu/Documents/in/Aedes_aegypti?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=48057","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="48057" href="https://www.academia.edu/Documents/in/DNA">DNA</a><script 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