Avi-tag and Amine Biotinylated Proteins from R&D Systems

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paragraph--type--hero-banner paragraph--view-mode--full paragraph-background-color--dark"> <div class="banner_background_image no-lazy-load" style="background: linear-gradient(to top, rgba(0, 0, 0, 0.0) 0%, rgba(0, 0, 0, 0.90) 100%), url('https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-avi-tag-protein-hero-banner-1500x340.png'); background-position: top center; background-repeat: no-repeat; background-size: cover;"> <div> <div class="paragraph paragraph--type--h1-2-2-text-to-media paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>Avi-tag and Amine-Labeled Biotinylated Proteins</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Biotinylated proteins can be powerful tools for assessing protein-protein interactions and screening  antibody or small molecule libraries for potential therapeutics. Whether you are looking for an Avi-tag or an amine-labeled biotinylated protein, Bio-Techne’s wide selection of R&D Systems™ biotinylated proteins will provide the performance you need and the consistency you expect to optimize your assay.</p></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_call_to_action_global"> <a href="#avi-tag" class="btn btn-primary" type="link"> Browse Biotinylated Proteins </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-avi-tag-protein-hero-img.png" alt="3D image of Avi-Tag protein" fetchpriority="high" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/reagents">Bio-Techne Reagents</a> </li> <li> <a href="/reagents/proteins">Proteins</a> </li> <li> Avi-tag Biotinylated Proteins </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-rampd-systems-biotinylated-proteins16" role="button" aria-expanded="false" aria-controls="expPara"> <h5>R&D Systems Biotinylated Proteins</h5> </a> </div> <div class="collapse" id="expPara-rampd-systems-biotinylated-proteins16" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p><strong>Choosing Between an Amine-Labeled or an Avi-tag Biotinylated Protein</strong></p> <p>Biotinylated proteins can be powerful tools for assessing protein-protein interactions and screening  antibody or small molecule libraries for potential therapeutics. Avi-tag recombinant proteins contain an extra 15 amino acid sequence, the Avi-tag, typically fused to the N- or the C-terminus of protein. This Avi-tag peptide is recognized by BirA ligase which enzymatically attaches a biotin molecule to a single lysine residue within the Avi-tag sequence. As a result, Avi-tag proteins are biotinylated in a site-specific manner, allowing for consistent, uniform protein biotinylation. This provides enhanced control of protein orientation in binding assays, and since the Avi-tag is located on the end of the protein, the effect on the activity of the protein is typically minimal. The result is a biotinylated protein that maintains the same characteristics as the unlabeled protein.</p> <p>In contrast to Avi-tag biotinylated proteins, amine-labeled biotinylated proteins are generatated by chemical biotinylation. This labels the protein on amine groups found at the N-terminus and in lysine residues throughout the protein, resulting in multiple biotins being incorporated per protein. The result is a biotinylated protein with a high binding efficiency to avidin or streptavidin and high signal strength.</p> <p>As biotin is a relatively small molecule, it typically does not affect the activity of the protein, but our in-house scientists rigorously test both our amine-labeled and Avi-tag biotinylated proteins to ensure that they exhibit the same level of bioactivity as the unlabeled protein. Additionally, the lot-to-lot consistency of all our proteins are routinely evaluated. Each new lot is tested side-by-side with previous lots and with a master lot, so you don’t have to worry whether your results will be reproducible over time. </p> <p>Explore our large selection of R&D Systems™ amine-labeled and Avi-tag biotinylated proteins to find the right protein for your assay.</p></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="sNhKLH3Z9gA" params="rel=0" style="background-image: url('https://img.youtube.com/vi/sNhKLH3Z9gA/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/sNhKLH3Z9gA?autoplay=1" class="lty-playbtn" title="R&D Systems Biotinylated Proteins"> <span class="lyt-visually-hidden">R&D Systems Biotinylated Proteins</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h3>R&D Systems Biotinylated Proteins Video</h3></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>This video describes how R&D Systems amine-labeled and Avi-tag biotinylated recombinant proteins are produced and the advantages associated with either chemical or enzymatic biotinylation.</p></div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3>Advantages of Avi-Tag Biotinylated Proteins</h3> <ul> <li> <p><strong>Consistent, highly specific labeling.</strong> A single biotin molecule is enzymatically added to a lysine residue in the Avi-tag by BirA biotin ligase, resulting in the generation of a homogeneous product.</p> </li> <li> <p><strong>Uniform orientation of the protein.</strong> When bound to a streptavidin-coated surface, the orientation of the Avi-tag biotinylated protein will be uniform due to the precise control over biotinylation.</p> </li> <li> <p><strong>Similar bioactivity as the unlabeled protein.</strong> All R&D Systems biotinylated proteins are rigorously tested to ensure that they exhibit the same level of bioactivity as the unlabeled protein.</p> </li> <li> <p><strong>Lot-to-lot consistency.</strong> Each new lot is tested side-by-side with previous lots and with a master lot, so you don’t have to worry whether your results will be reproducible over time.</p> </li> </ul></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="avi-tag"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3>Avi-tag Proteins – Products by Molecule</h3> <figure class="responsive-figure-table"><table class="overflow-auto table table-bordered"> <tbody> <tr> <td><a href="/t/2b4-cd244-slamf4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">2B4/CD244/SLAMF4</a></td> <td><a href="/t/4-1bb-tnfrsf9-cd137?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">4-1BB/TNFRSF9</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-5-nucleotidase-cd73-his-tag-avi-tag-cf_avi5795">5'-Nucleotidase/CD73</a></td> <td><a href="/t/5t4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">5T4</a></td> <td><a href="/t/ace-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ACE-2</a></td> <td><a href="/t/alcam-cd166?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ALCAM/CD166</a></td> </tr> <tr> <td><a href="/t/axl?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Axl</a></td> <td><a href="/t/b7-1-cd80?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">B7-1/CD80</a></td> <td><a href="/t/b7-2-cd86?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">B7-2/CD86</a></td> <td><a href="/t/b7-h2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">B7-H2</a></td> <td><a href="/t/b7-h3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">B7-H3</a></td> <td><a href="/t/b7-h4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">B7-H4</a></td> </tr> <tr> <td><a href="/t/b7-h6?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">B7-H6</a></td> <td><a href="/t/bcma-tnfrsf17?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">BCMA</a></td> <td><a href="/t/btn1a1-butyrophilin?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">BTN1A1/Butyrophilin</a></td> <td><a href="/t/btn2a1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">BTN2A1</a></td> <td><a href="/t/btn3a2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">BTN3A2</a></td> <td><a href="/t/cd3-delta-cd3d?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD3 delta</a></td> </tr> <tr> <td><a href="/t/cd4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD4</a></td> <td><a href="/t/cd6?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD6</a></td> <td><a href="/t/cd8?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD8 alpha</a></td> <td><a href="/t/cd19?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD19</a></td> <td><a href="/t/cd24?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD24</a></td> <td><a href="/t/cd25-il-2r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD25/IL-2 R</a></td> </tr> <tr> <td><a href="/t/cd27-tnfrsf7?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD27</a></td> <td><a href="/t/cd28?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD28</a></td> <td><a href="/t/cd30-tnfrsf8?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD30/TNFRSF8</a></td> <td><a href="/t/cd38?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD38</a></td> <td><a href="/t/cd40-tnfrsf5?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD40/TNFRSF5</a></td> <td><a href="/t/cd44?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD44v5</a></td> </tr> <tr> <td><a href="/t/cd47?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD47</a></td> <td><a href="/t/cd48-slamf2?sub_category=Recombinant%20Proteins&conjugate_form=Biotin">CD48/SLAMF2</a></td> <td><a href="/t/cd117-c-kit?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD117/c-kit</a></td> <td><a href="/t/cd155-pvr?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD155/PVR</a></td> <td><a href="/t/cd200?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD200</a></td> <td><a href="/t/cd200r1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CD200 R1</a></td> </tr> <tr> <td><a href="/t/ceacam-1-cd66a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CEACAM-1/CD66a</a></td> <td><a href="/t/ceacam-5-cd66e?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CEACAM-5/CD66e</a></td> <td><a href="/t/ceacam-6-cd66c?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CEACAM-6/CD66c</a></td> <td><a href="/t/common-gamma-chain-il-2-r-gamma?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Common gamma chain</a></td> <td><a href="/t/ctla-4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CTLA-4</a></td> <td><a href="/t/cracc-slamf7?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CRACC/SLAMF7</a></td> </tr> <tr> <td><a href="/t/cx3cl1-fractalkine?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">CX3CL1/Fractalkine</a></td> <td><a href="/t/dll4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">DLL4</a></td> <td><a href="/t/dnam-1-cd226?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">DNAM-1/CD226</a></td> <td><a href="/t/dppiv-cd26?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">DPPIV/CD26</a></td> <td><a href="/t/egfr?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">EGFR</a></td> <td><a href="/t/epcam-trop1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">EpCAM/TROP1</a></td> </tr> <tr> <td><a href="/t/erbb2-her2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ErbB2/Her2</a></td> <td><a href="/t/erbb3-her3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ErbB3/Her3</a></td> <td><a href="/t/fc-epsilon-ri-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Fc epsilon RI alpha</a></td> <td><a href="/t/fc-gamma-riib-c-cd32b-c?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Fc gamma RIIB/CD32b</a></td> <td><a href="/t/fc-gamma-riia-cd32a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Fc gamma RIIA/CD32a</a></td> <td><a href="/t/fc-gamma-riiia-cd16a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Fc gamma RIIIA</a></td> </tr> <tr> <td><a href="/t/fgfr1-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">FGFR1 alpha (IIIc)</a></td> <td><a href="/t/fgfr2-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">FGFR2 alpha (IIIc)</a></td> <td><a href="/t/fgfr3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">FGFR3 </a></td> <td><a href="/t/fgfr4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">FGFR4</a></td> <td><a href="/t/flt-3-flk-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Flt-3/Flk-2</a></td> <td><a href="/t/gitr-tnfrsf18?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">GITR/TNFRSF18</a></td> </tr> <tr> <td><a href="/t/glypican-3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Glypican-3</a></td> <td><a href="/t/gp130?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">gp130</a></td> <td><a href="/t/guanylyl-cyclase-c-gucy2c?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Guanylyl Cyclase C</a></td> <td><a href="/t/hepassocin-fgl1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Hepassocin/FGL1</a></td> <td><a href="/t/hvem-tnfrsf14?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">HVEM/TNFRSF14</a></td> <td><a href="/t/icam-1-cd54?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ICAM-1/CD54</a></td> </tr> <tr> <td><a href="/t/icos?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ICOS</a></td> <td><a href="/t/ifn-gamma-r1-cd119?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IFN-gamma R1/CD119</a></td> <td><a href="/t/igg1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IgG1</a></td> <td><a href="/t/il-1-racp-il-1-r3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-1 RAcP/IL-1 R3</a></td> <td><a href="/t/il-1rrp2-il-1r6?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-1 Rrp2/IL-1R6</a></td> <td><a href="/t/il-2-r-beta?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-2 R beta</a></td> </tr> <tr> <td><a href="/t/il-3r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-3R alpha</a></td> <td><a href="/t/il-4r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-4 R alpha</a></td> <td><a href="/t/il-5-r-alpha-cd125?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-5R alpha/CD125</a></td> <td><a href="/t/il-6r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-6 R alpha</a></td> <td><a href="/t/il-7r-alpha-cd127?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-7 R alpha/CD127</a></td> <td><a href="/t/il-10-r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-10 R alpha</a></td> </tr> <tr> <td><a href="/t/il-12-r-beta-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-12 R beta 1</a></td> <td><a href="/t/il-12-r-beta-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-12 R beta 2</a></td> <td><a href="/t/il-13-r-alpha-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-13 R alpha 1</a></td> <td><a href="/t/il-15r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-15 R alpha</a></td> <td><a href="/t/il-17ra-il-17r?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL17RA/IL-17R</a></td> <td><a href="/t/il-18-bpa?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-18 Bpa</a></td> </tr> <tr> <td><a href="/t/il-18-r-alpha-il-1-r5?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-18 R alpha/IL-1 R5</a></td> <td><a href="/t/il-18-r-beta-il-1-r7?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-18 R beta/IL-1 R7</a></td> <td><a href="/t/il-21r?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-21 R</a></td> <td><a href="/t/il-23r?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-23R</a></td> <td><a href="/t/il-31ra?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-31RA</a></td> <td><a href="/t/jagged-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Jagged 1</a></td> </tr> <tr> <td><a href="/t/lag-3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">LAG-3</a></td> <td><a href="/t/lair1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">LAIR1</a></td> <td><a href="/t/lair2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">LAIR2</a></td> <td><a href="/t/lilrb2-cd85d-ilt4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">LILRB2/CD85d/ILT4</a></td> <td><a href="/t/lymphotoxin?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Lymphotoxin alpha1/beta2</a></td> <td><a href="/t/m-csf-r-cd115?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">M-CSF R/CD115</a></td> </tr> <tr> <td><a href="/t/mer?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Mer</a></td> <td><a href="/t/mesothelin?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Mesothelin</a></td> <td><a href="/t/mica?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">MICA</a></td> <td><a href="/t/mspr-ron?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">MSPR/Ron</a></td> <td><a href="/t/muc-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">MUC-1</a></td> <td><a href="/t/musk?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">MuSK</a></td> </tr> <tr> <td><a href="/t/nectin-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Nectin-1</a></td> <td><a href="/t/nectin-2-cd112?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Nectin-2/CD112</a></td> <td><a href="/t/nectin-4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Nectin-4</a></td> <td><a href="/p/proteins-enzymes/recombinant-human-neuropilin-1-fc-avi-tag-protein-cf_avi10455">Neuropilin-1</a></td> <td><a href="/t/nkp30-ncr3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">NKp30</a></td> <td><a href="/t/nkp44-ncr2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Unconjugated">NKp44/NCR2</a></td> </tr> <tr> <td><a href="/t/osteoactivin-gpnmb?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Osteoactivin/GPNMB</a></td> <td><a href="/t/ox40-tnfrsf4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">OX40/TNFRSF4</a></td> <td><a href="/t/pd-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PD-1</a></td> <td><a href="/t/pd-l1-b7-h1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PD-L1/B7-H1</a></td> <td><a href="/t/pd-l2-b7-dc?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PD-L2/B7-DC</a></td> <td><a href="/t/pdgf-r-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PDGF R alpha</a></td> </tr> <tr> <td><a href="/t/pdgf-r-beta?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PDGF R beta</a></td> <td><a href="/t/pilr-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PILR-alpha</a></td> <td><a href="/t/pilr-beta?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PILR-beta</a></td> <td><a href="/t/psma-folh1-naaladase-i?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PSMA/FOLH1/ NAALADase I</a></td> <td><a href="/t/ror1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ROR1</a></td> <td><a href="/t/siglec-2-cd22?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Siglec-2/CD22</a></td> </tr> <tr> <td><a href="/t/siglec-3-cd33?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Siglec-3/CD33</a></td> <td><a href="/t/siglec-10?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Siglec-10</a></td> <td><a href="/t/sirp-alpha-cd172a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SIRP alpha/CD172a</a></td> <td><a href="/t/spike?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SARS-CoV-2 Spike</a></td> <td><a href="/t/spike-rbd?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SARS-CoV-2 Spike RBD</a></td> <td><a href="/t/spike-s1-subunit?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SARS-CoV-2 Spike S1</a></td> </tr> <tr> <td><a href="/t/st2-il-33r?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ST2/IL-33 R</a></td> <td><a href="/t/syndecan-1-cd138?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Syndecan-1</a></td> <td><a href="/t/tgf-beta-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TGF-beta 1</a></td> <td><a href="/t/tgf-beta-ri-alk-5?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TGF-beta RI</a></td> <td><a href="/t/tgf-beta-rii?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TGF-beta RII</a></td> <td><a href="/t/tie-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Tie-2</a></td> </tr> <tr> <td><a href="/t/tigit?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TIGIT (T103)</a></td> <td><a href="/t/tim-1-kim-1-havcr?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TIM-1/KIM-1/ HAVCR</a></td> <td><a href="/t/tim-3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TIM-3</a></td> <td><a href="/t/tnf-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TNF-alpha</a></td> <td><a href="/t/tnf-ri-tnfrsf1a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TNF RI/TNFRSF1A</a></td> <td><a href="/t/tnf-rii-tnfrsf1b?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TNF RII/TNFRSF1B</a></td> </tr> <tr> <td><a href="/t/trance-tnfsf11-rank-l?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TRANCE/RANK L</a></td> <td><a href="/t/trem-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TREM-1</a></td> <td><a href="/t/trem2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TREM2</a></td> <td><a href="/t/tslpr?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TSLP R</a></td> <td><a href="/t/upar?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">uPAR</a></td> <td><a href="/t/ulbp-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ULBP-2</a></td> </tr> <tr> <td><a href="/t/vegf?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VEGF 165</a></td> <td><a href="/t/vegfr2-kdr-flk-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VEGFR2/KDR/ Flk-1</a></td> <td><a href="/t/vegfr3-flt-4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VEGFR3/Flt-4</a></td> <td><a href="/t/vista-b7-h5-pd-1h?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VISTA/B7-H5/PD-1H</a></td> <td><a href="/t/vsig3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VSIG3</a></td> <td><a href="/t/vsig4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VSIG4</a></td> </tr> <tr> <td><a href="/t/vstm2a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VSTM2A</a></td> <td><a href="/t/vstm2b?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VSTM2B</a></td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <a href="/search?keywords=avi&category_attr=Proteins%20and%20Enzymes" > <button type="button" class="btn btn-primary"> Search All Avi-tag Proteins </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3>Advantages of Amine-Labeled Biotinylated Proteins</h3> <p><strong>• High signal strength. </strong>Chemical biotinylation labels the protein on amine groups found in lysine residues throughout the protein and at the N-terminus, resulting in multiple biotins being incorporated per protein.</p> <p>• <strong>Similar bioactivity as the unlabeled protein.</strong> All R&D Systems biotinylated proteins are rigorously tested to ensure that they exhibit the same level of bioactivity as the unlabeled protein.</p> <p>• <strong>Lot-to-lot consistency.</strong> Each new lot is tested side-by-side with previous lots and with a master lot, so you don’t have to worry whether your results will be reproducible over time. </p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3>Amine-Labeled Biotinylated Proteins - Products by Molecule</h3> <figure class="responsive-figure-table"><table class="overflow-auto table table-bordered"> <tbody> <tr> <td><a href="/t/ace-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">ACE-2</a></td> <td><a href="/t/age-bsa?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">AGE-BSA</a></td> <td><a href="/t/bmp-7?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">BMP-7</a></td> <td><a href="/t/ephrin-a1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-A1</a></td> <td><a href="/t/ephrin-a2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-A2</a></td> <td><a href="/t/ephrin-a3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-A3</a></td> </tr> <tr> <td><a href="/t/ephrin-a4?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-A4</a></td> <td><a href="/t/ephrin-a5?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-A5</a></td> <td><a href="/t/ephrin-b1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-B1</a></td> <td><a href="/t/ephrin-b2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-B2</a></td> <td><a href="/t/ephrin-b3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ephrin-B3</a></td> <td><a href="/t/frizzled-8?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Frizzled-8</a></td> </tr> <tr> <td><a href="/t/hgf?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">HGF</a></td> <td><a href="/t/il-2?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-2</a></td> <td><a href="/t/il-21?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-21</a></td> <td><a href="/t/il-27?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">IL-27</a></td> <td><a href="/t/lag-3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">LAG-3</a></td> <td><a href="/t/lif?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">LIF</a></td> </tr> <tr> <td><a href="/t/m-csf?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">M-CSF</a></td> <td><a href="/t/pd-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PD-1</a></td> <td><a href="/t/pd-l1-b7-h1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PD-L1</a></td> <td><a href="/t/pdgf-bb?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">PDGF-BB</a></td> <td><a href="/t/spike?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SARS-CoV-2 spike</a></td> <td><a href="/t/spike-rbd?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SARS-CoV-2 Spike RBD</a></td> </tr> <tr> <td><a href="/t/scf-c-kit-ligand?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SCF</a></td> <td><a href="/t/sirp-alpha-cd172a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">SIRP-alpha</a></td> <td><a href="/t/tnf-alpha?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TNF-alpha</a></td> <td><a href="/t/tgf-beta-1?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">TGF beta</a></td> <td><a href="/t/ubiquitin?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Ubiquitin</a></td> <td><a href="/t/vegf?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VEGF</a></td> </tr> <tr> <td><a href="/t/vista-b7-h5-pd-1h?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VISTA</a></td> <td><a href="/t/vsig3?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">VSIG3</a></td> <td><a href="/t/wnt-3a?category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin">Wnt-3a</a></td> <td><a href="/t/wnt-5a?category_attr=Proteins%20and%20Enzymes">Wnt-5a</a></td> <td> </td> <td> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <a href="/search?keywords=biotinylated&category_attr=Proteins%20and%20Enzymes&conjugate_form=Biotin" > <button type="button" class="btn btn-primary"> Search All Biotinylated Proteins </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h3>R&D Systems Biotinylated Proteins Exhibit the Same Levels of Bioactivity as the Equivalent Unlabeled Proteins</h3></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/recombinant-human-pd-l1-b7-h1-graph-v3.png" alt="R&D Systems Avi-tag Biotinylated Recombinant Human PD-L1 has equivalent bioactivity as unlabeled Recombinant Human PD-L1 " loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><strong>Unlabeled and Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 Display Comparable Bioactivity.</strong> Human T lymphocytes were treated with the indicated concentrations of either unlabeled <a href="/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-fc-chimera-protein-cf_156-b7">Recombinant Human PD-L1/B7-1 Fc Chimera</a> (R&D Systems, Catalog # 156-B7; green line) or <a href="/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-fc-chimera-avi-tag-protein-cf_avi156">Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera</a> (R&D Systems, Catalog # AVI156; orange line). IL-2 secretion was measured in cell culture supernatants using the <a href="/p/elisa-kits/human-il-2-quantikine-elisa-kit_d2050">Human IL-2 Quantikine® ELISA Kit</a> (R&D Systems, Catalog # D2050). The similarity in the activities of the two proteins highlights that the Avi-tag biotinylated protein is fully functional.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-unlabeled-and-amine-labeled-biotinylated-recombinant-human-tnf-alpha-bioactivity-comparison-data8" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Unlabeled and Amine-Labeled Biotinylated Recombinant Human TNF-alpha - Bioactivity Comparison Data</h5> </a> </div> <div class="collapse" id="expPara-unlabeled-and-amine-labeled-biotinylated-recombinant-human-tnf-alpha-bioactivity-comparison-data8" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/rnd-biotinylated-human-tnf-alpha-graph.png" alt="R&D Systems Biotinylated Recombinant Human TNF-alpha has equivalent bioactivity as unlabeled Recombinant Human TNF-alpha" loading="lazy" > </div> <br/> <div><p><strong>Unlabeled and Biotinylated Recombinant Human TNF-alpha Display Comparable Bioactivity in an Assay Measuring Cytotoxicity in L-929 Mouse Fibroblasts.</strong> L-929 mouse fibroblast cells were treated with the indicated concentrations of either unlabeled <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-protein_210-ta">Recombinant Human TNF-alpha</a> (R&D Systems, Catalog # 210-TA; green line) or <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-biotinylated-protein_bt210">Biotinylated Recombinant Human TNF-alpha</a> (R&D Systems, Catalog # BT210; orange line), in the presence of the metabolic inhibitor, actinomycin D, and cytotoxicity was assessed. The ED<sub>50</sub> for this effect is 25-100 pg/mL. The similarity in the activities of the two proteins highlights that the biotinylated protein is fully functional.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-unlabeled-and-amine-labeled-biotinylated-recombinant-human-pdgf-bb-bioactivity-comparison-data21" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Unlabeled and Amine-Labeled Biotinylated Recombinant Human PDGF-BB - Bioactivity Comparison Data</h5> </a> </div> <div class="collapse" id="expPara-unlabeled-and-amine-labeled-biotinylated-recombinant-human-pdgf-bb-bioactivity-comparison-data21" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/rnd-biotinylated-human-pdgf-bb-graph.png" alt="R&D Systems Biotinylated Recombinant Human PDGF-BB has equivalent bioactivity as unlabeled Recombinant Human PDGF-BB" loading="lazy" > </div> <br/> <div><p><strong>Unlabeled and Biotinylated Recombinant Human PDGF-BB Display Comparable Bioactivity in an Assay Measuring Proliferation of NR6R-3T3 Mouse Fibroblast Cells.</strong> NR6R-3T3 mouse fibroblast cells were treated with the indicated concentrations of either unlabeled <a href="/p/proteins-enzymes/recombinant-human-pdgf-bb-protein-cf_220-bb">Recombinant Human PDGF-BB</a> (R&D Systems, Catalog # 220-BB; green line) or <a href="/p/proteins-enzymes/recombinant-human-pdgf-bb-biotinylated-protein_bt220">Biotinylated Recombinant Human PDGF-BB</a> (R&D Systems, Catalog # BT220; orange line) and cell proliferation was assessed. The similarity in the activities of the two proteins highlights that the biotinylated protein is fully functional.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h3>R&D Systems Biotinylated Proteins Are Rigorously Tested to Ensure Lot-to-lot Consistency</h3></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong>R&D Systems Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 Displays High Lot-to-Lot Consistency.</strong> Three independent lots of <a href="/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-fc-chimera-avi-tag-protein-cf_avi156">Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1</a> (R&D Systems, Catalog # AVI156) were tested for their ability to bind to <a href="/p/proteins-enzymes/recombinant-human-pd-1-fc-chimera-protein-cf_1086-pd">Recombinant Human PD-1</a> (R&D Systems, Catalog # 1086-PD), which was coated at 1 ug/mL. Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 bound with an ED<sub>50</sub> of 8-48 ng/mL. Each trace shown on the graph represents data obtained from Avi-tag Biotinylated Recombinant Human PD-L1/B7-H1 from a different manufacturing run to show the lot-to-lot consistency of the proteins.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/recombinant-human-pd-l1-b7-h1-lot-to-lot-consistency-graph-v4.png" alt="Analysis of the lot-to-lot consistency of R&D Systems Avi-tag Biotinylated Recombinant Human PD-L1 Protein" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div></div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <a href="https://www.rndsystems.com/resources/articles/avi-tag-biotinylated-proteins-app-note" > <button type="button" class="btn btn-primary"> Download the Avi-tag Proteins Application Note </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default"> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h3>Analysis of the Binding Properties of R&D Systems Avi-tag Biotinylated Proteins</h3></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/pd-1-sersorgrams-data.jpg" alt="Surface plasmon resonance data showing the affinity measurements and binding kinetics between the PD-L1 and PD-1 proteins." loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><strong>Affinity Measurements and Binding Kinetics of the PD-1:PD-L1 Interaction by Surface Plasmon Resonance.</strong> Sensorgram data of captured <a href="/p/proteins-enzymes/recombinant-human-pd-l1-b7-h1-his-tag-avi-tag-protein-cf_avi9049">Avi-tag Biotinylated Recombinant Human PD-L1 His tag</a> (R&D Systems, Catalog # AVI9049) binding to <a href="/p/proteins-enzymes/recombinant-human-pd-1-his-tagged-protein-cf_8986-pd">Recombinant Human PD-1 His tag</a> (R&D Systems, Catalog # 8986-PD). The corresponding overlaid kinetic fits with the residual plot shown below. The concentration of Recombinant Human PD-1 His-tag ranged from 3.2 nM to 13.2 μM. The corresponding steady state affinity fit is shown below. The experiment was performed on a BiacoreT200, GE Healthcare.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-avi-tag-biotinylated-recombinant-human-ctla-4-and-b7-1-spr-data3" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Avi-tag Biotinylated Recombinant Human CTLA-4 and B7-1 - SPR Data</h5> </a> </div> <div class="collapse" id="expPara-avi-tag-biotinylated-recombinant-human-ctla-4-and-b7-1-spr-data3" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/ctla-4-b7-1-data-vertical.jpg" alt="Surface plasmon resonance data showing the affinity measurements and binding kinetics between the CTLA-4 and B7-1 proteins." loading="lazy" > </div> <br/> <div><p><strong>Affinity Measurements and Binding Kinetics of the CTLA-4:B7-1 Interaction by Surface Plasmon Resonance.</strong> Sensorgram data of captured <a href="/p/proteins-enzymes/recombinant-human-ctla-4-fc-chimera-avi-tag-protein-cf_avi7268">Avi-tag Biotinylated Recombinant Human CTLA-4 Fc Chimera</a> (R&D Systems, Catalog # AVI7268) binding to <a href="/p/proteins-enzymes/recombinant-human-b7-1-cd80-fc-chimera-protein-cf_10133-b1">Recombinant Human B7-1 Fc Chimera</a> (R&D Systems, Catalog # 10133-B1). Briefly, Avi-tag Biotinylated Recombinant Human CTLA-4 Fc Chimera was captured at a low coupling density to the active flow cell via the Avi-tag biotin. Recombinant Human B7-1 Fc Chimera at a concentration range between 82 pM and 21 nM was flowed over both active and uncoupled reference flow cells at each concentration. Kinetic sensorgrams were fit to a 1:1 binding model to determine the binding kinetics and affinity, with an interaction affinity of KD=0.2511 nM. The corresponding overlaid kinetic fits with the residual plot shown below. The corresponding steady state affinity fit is shown at the bottom. The experiment was performed on a Biacore T200, GE Healthcare.</p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-avi-tag-biotinylated-recombinant-human-cd47-and-sirp-alpha-spr-data2" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Avi-tag Biotinylated Recombinant Human CD47 and SIRP-alpha - SPR Data</h5> </a> </div> <div class="collapse" id="expPara-avi-tag-biotinylated-recombinant-human-cd47-and-sirp-alpha-spr-data2" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cd-47-sirp-alpha-v2.jpg" alt="Surface plasmon resonance data showing affinity measurements and binding kinetics between the CD47 and SIRP alpha proteins." loading="lazy" > </div> <br/> <div><p><strong>Affinity Measurements and Binding Kinetics of the CD47:SIRP alpha Interaction by Surface Plasmon Resonance. </strong>Sensorgram data of captured <a href="/p/proteins-enzymes/recombinant-human-cd47-fc-chimera-avi-tag-protein-cf_avi4670">Avi-tag Biotinylated Recombinant Human CD47 Fc tag</a> (R&D Systems, Catalog # AVI4670)binding to <a href="/p/proteins-enzymes/recombinant-human-sirp-alpha-cd172a-his-tag-protein-cf_9378-sa">Recombinant Human SIRP alpha His tag</a> (R&D Systems, Catalog # 9378-SA). The corresponding overlaid kinetic fits with the residual plot shown below. The concentration of Recombinant Human SIRP alpha His-tag ranged from 6.85 nM to 3.51 μM. The corresponding steady state affinity fit is shown at the bottom. The experiment was performed on a Biacore T200, GE Healthcare. </p></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-avi-tag-biotinylated-recombinant-human-cd155pvr-and-tigit-spr-data15" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Avi-tag Biotinylated Recombinant Human CD155/PVR and TIGIT - SPR Data</h5> </a> </div> <div class="collapse" id="expPara-avi-tag-biotinylated-recombinant-human-cd155pvr-and-tigit-spr-data15" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/pd-l1-spr-data.jpg" alt="Surface plasmon resonance data showing the affinity measurements and binding kinetics between the CD155 and TIGIT proteins." loading="lazy" > </div> <br/> <div><p><strong>Affinity Measurements and Binding Kinetics of the CD155/PVR:TIGIT Protein Interaction by Surface Plasmon Resonance. </strong>Sensorgram data of captured<a href="/p/proteins-enzymes/recombinant-human-cd155-pvr-fc-chimera-avi-tag-protein-cf_avi9174"> Avi-tag Biotinylated Recombinant Human CD155/PVR Fc Chimera</a> (R&D Systems, Catalog # AVI9174) binding to <a href="/p/proteins-enzymes/recombinant-human-tigit-his-tag-protein-cf_9525-tg">Recombinant Human TIGIT His-tag</a> (R&D Systems, Catalog # 9525-TG). The corresponding overlaid kinetic fits with the residual plot shown below. The concentration of Recombinant Human TIGIT His-tag ranged from 0.2 nM to 400 nM. The corresponding steady state affinity fit is shown at the bottom. The experiment was performed on a Biacore T200, GE Healthcare.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Additional Resources</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/immune-checkpoint-targets-for-cancer-immunotherapy-research-poster" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/io-poster-teaser.jpg" alt="I/O Poster Teaser" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/immune-checkpoint-targets-for-cancer-immunotherapy-research-poster" > <h3 class="card_title_htag"> <div><h3>Wall Poster: Immune Checkpoint Targets</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Immune checkpoint molecules deliver signals that control the activities of a variety of immune cell types and are some of the most highly investigated targets for immuno-oncology research. Use our new wall poster to explore the current and emerging immune checkpoint molecules being investigated as potential cancer immunotherapy targets.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/immune-checkpoint-targets-for-cancer-immunotherapy-research-poster" > Request a Free Poster </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/current-and-emerging-immune-checkpoint-targets-immuno-oncology-research-ebook#request-ebook" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/chemokine-blocking-teaser-bg.jpg" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/literature/v10_new-io-ebook-thumb.png" alt="Immune-Oncology eBook" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/current-and-emerging-immune-checkpoint-targets-immuno-oncology-research-ebook#request-ebook" > <h3 class="card_title_htag"> <div><h3>eBook: Current and Emerging Immune Checkpoint Targets for I-O Research</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Immune checkpoint proteins play a central role in regulating the activities of different immune cell types and represent some of the most promising targets for cancer immunotherapy. This eBook highlights some of the immune checkpoint molecules that are being investigated as immuno-oncology targets, and the products that we offer for studying these molecules.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/current-and-emerging-immune-checkpoint-targets-immuno-oncology-research-ebook#request-ebook" > Download the eBook </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://www.rndsystems.com/resources/posters/look-inside-tumor-mechanism-tumor-evasion-and-immunosuppression-tumor" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/poster-525x315.jpg" alt="poster A Look Inside A Tumor 525x315" loading="lazy" > </div> </a> <a class="card_title_link" href="https://www.rndsystems.com/resources/posters/look-inside-tumor-mechanism-tumor-evasion-and-immunosuppression-tumor" > <h3 class="card_title_htag"> <div><h3>Wall Poster: A Look Inside a Tumor</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>The tumor microenvironment (TME) plays a central role in inhibiting anti-tumor immune responses. Use our poster to learn about the key mechanisms by which tumor cells, tumor-derived exosomes, regulatory T cells, myeloid-derived suppressor cells (MDSCs), and tumor-associated macrophages (TAMs) mediate immunosuppression in the tumor microenvironment.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://www.rndsystems.com/resources/posters/look-inside-tumor-mechanism-tumor-evasion-and-immunosuppression-tumor" > Request Your Wall Poster </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Related Protein Products</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/proteins/immune-checkpoint-proteins" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/illustration-final-7.jpg" alt="TIGIT and DNAM-1 both bind to CD155 and CD112, but have opposing effects on the functions of T cells and NK cells." loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/proteins/immune-checkpoint-proteins" > <h3 class="card_title_htag"> <div><h3>Immune Checkpoint Proteins</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>R&D Systems offers an extensive range of bioactive immune checkpoint proteins. In addition to known proteins such as PD-1, PD-L1, CTLA-4, and CD28, we offer many lesser-known immune checkpoint proteins that exhibit similar co-inhibitory effects.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/proteins/immune-checkpoint-proteins" > Learn More About Immune Checkpoint Proteins </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/proteins/immune-cell-culture-proteins" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/banners/v2_immune-cell-culture-backlinking_teaser-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/banners/immune-cell-culture-backlinking4_teaser-foreground.png" alt="Macrophage Immune Cell" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/proteins/immune-cell-culture-proteins" > <h3 class="card_title_htag"> <div>Proteins for Immune Cell Culture</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Achieve robust, reproducible immune cell cultures with R&D Systems™ proteins. Our proteins are rigorously tested to ensure that they will provide superior performance and lot-to-lot consistency, so you can have confidence in their ability to promote optimal immune cell expansion and differentiation with minimal variability between cultures.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/proteins/immune-cell-culture-proteins" > Browse Our Proteins for Immune Cell Culture </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/proteins/fluorokines-fluorescent-labeled-proteins" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/fluorokines-car-t-teaser-350x215.png" alt="Fluorokines for CAR-T" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/proteins/fluorokines-fluorescent-labeled-proteins" > <h3 class="card_title_htag"> <div>Fluorescent Proteins for CAR-T Research</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Fluorescent labeled CD19, BCMA, and Siglec-2 allow for direct detection of a corresponding chimeric antigen receptor (CAR) on cells. 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Avi-tag and Amine Biotinylated Proteins from R&D Systems