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(PDF) Hair testing for cortisol by UPLC–MS/MS in a family: External cross-contamination from use of cortisol cream
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Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.","publication_date":"2019,12,1","publication_name":"Forensic Science International","grobid_abstract_attachment_id":"112034826"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Hair testing for cortisol by UPLC–MS/MS in a family: External cross-contamination from use of cortisol cream","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [38476187]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon'; window.userInChina = "false";</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":112034826,"attachmentType":"pdf"}"><img alt="First page of “Hair testing for cortisol by UPLC–MS/MS in a family: External cross-contamination from use of cortisol cream”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/112034826/mini_magick20240303-1-ccsrgx.png?1709446307" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/images/single_work_splash/adobe_icon.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Hair testing for cortisol by UPLC–MS/MS in a family: External cross-contamination from use of cortisol cream</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="38476187" href="https://independent.academia.edu/SysJohansen"><img alt="Profile image of Sys Johansen" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Sys Johansen</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2019, Forensic Science International</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">30 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 115701750; 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However, cortisol levels are subject to variations due to acute stress, the diurnal rhythm and pulsatile secretion. Cortisol measurements in body fluids are not always a reflection of long-term cortisol exposure. The analysis of cortisol in scalp hair is a relatively novel method to measure cumulative cortisol exposure over months up to years. Over the past years, hair cortisol concentrations (HCC) have been examined in association with a large number of somatic and mental health conditions. HCC can be used to evaluate disturbances of the HPA axis, including Cushing&#39;s syndrome, and to evaluate hydrocortisone treatment. Using HCC, retrospective timelines of cortisol exposure can be created, which can be of value in diagnosing cyclic hypercortisolism. HCC have also been shown to increase with psychological stressors, includi...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Clinical applications of cortisol measurements in hair","attachmentId":44137817,"attachmentType":"pdf","work_url":"https://www.academia.edu/23715261/Clinical_applications_of_cortisol_measurements_in_hair","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/23715261/Clinical_applications_of_cortisol_measurements_in_hair"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="70600625" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/70600625/Validity_and_reliability_of_method_used_to_analyse_hair_cortisol_concentration">Validity and reliability of method used to analyse hair cortisol concentration</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="185230481" href="https://tudublin.academia.edu/JordanReid">Jordan Reid</a></div><p class="ds-related-work--metadata ds2-5-body-xs">F1000Research</p><p class="ds-related-work--abstract ds2-5-body-sm">Hair cortisol analysis is a method of analysing the stress hormone cortisol that offers great potential for helping researchers understand the long-term impact of stress and distress on the body. Hair analysis not only provides an excellent method of studying the average production of cortisol over weeks and months, but also the potential to understand cortisol levels several months before the hair was collected. Whilst research with hair samples for cortisol analysis is a fast-developing field, there has been less analysis of the methods used to analyse hair cortisol. We report two studies where the novel hair analysis method developed at the Anglia Ruskin university (ARU) Biomarker Laboratory was tested for reliability and validity. In study 1, 32 participants provided hair samples for an examination of the reproducibility of the hair cortisol analysis method. In study 2, 53 participants provided a hair sample cut from the scalp, and the methanol that the cortisol was extracted in...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Validity and reliability of method used to analyse hair cortisol concentration","attachmentId":80284640,"attachmentType":"pdf","work_url":"https://www.academia.edu/70600625/Validity_and_reliability_of_method_used_to_analyse_hair_cortisol_concentration","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/70600625/Validity_and_reliability_of_method_used_to_analyse_hair_cortisol_concentration"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="23715251" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/23715251/Toward_Standardization_of_Hair_Cortisol_Measurement_Results_of_the_First_International_Inter_laboratory_Round_Robin">Toward Standardization of Hair Cortisol Measurement; Results of the First International Inter-laboratory Round Robin</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33049582" href="https://independent.academia.edu/ElisabethVanRossum">Elisabeth Van Rossum</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Therapeutic Drug Monitoring, 2014</p><p class="ds-related-work--abstract ds2-5-body-sm">Background: The importance of hair cortisol as a long-term, retrospective measure of systemic cortisol exposure is being increasingly recognized, and over recent years the field of hair cortisol analysis has seen rapid expansion with laboratories around the globe integrating hair cortisol analysis into their study designs. These laboratories employ different methods of analysis and presently no attempt has been made to compare them.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Toward Standardization of Hair Cortisol Measurement; Results of the First International Inter-laboratory Round Robin","attachmentId":44137809,"attachmentType":"pdf","work_url":"https://www.academia.edu/23715251/Toward_Standardization_of_Hair_Cortisol_Measurement_Results_of_the_First_International_Inter_laboratory_Round_Robin","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/23715251/Toward_Standardization_of_Hair_Cortisol_Measurement_Results_of_the_First_International_Inter_laboratory_Round_Robin"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="113655874" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/113655874/Hair_cortisol_analysis_An_update_on_methodological_considerations_and_clinical_applications">Hair cortisol analysis: An update on methodological considerations and clinical applications</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="36470223" href="https://independent.academia.edu/StanVanUum">Stan Van Uum</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinical biochemistry, 2018</p><p class="ds-related-work--abstract ds2-5-body-sm">Hair cortisol analysis is increasingly being appreciated and applied in both research and medicine, aiding endocrinologists with diagnosis. We provide an overview of hair cortisol research in general and an update on methodological considerations including the incorporation of cortisol into hair, hair growth rates, and sampling procedures, mincing vs. grinding of samples during preparation for extraction, various extraction protocols, and quantification techniques. We compare the clinical utility and application of hair cortisol with traditional methods of measurement while acknowledging the limitations of analysis including variations in hair growth parameters. We explore the value of hair cortisol in cases of Cushing syndrome (particularly Cyclical Cushing), Adrenal insufficiency (including Addison&#39;s disease), therapy monitoring, cardiovascular disease, stress, and mental illness. Hair cortisol provides a unique objective biomarker for the analysis of endogenous cortisol level...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Hair cortisol analysis: An update on methodological considerations and clinical applications","attachmentId":110560284,"attachmentType":"pdf","work_url":"https://www.academia.edu/113655874/Hair_cortisol_analysis_An_update_on_methodological_considerations_and_clinical_applications","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/113655874/Hair_cortisol_analysis_An_update_on_methodological_considerations_and_clinical_applications"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="48191846" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/48191846/An_assessment_of_cortisol_analysis_in_hair_and_its_clinical_applications">An assessment of cortisol analysis in hair and its clinical applications</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32710843" href="https://independent.academia.edu/MichaelRieder">Michael Rieder</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Forensic Science International, 2010</p><p class="ds-related-work--abstract ds2-5-body-sm">Hair analyses for exogenous compounds, specifically drugs of abuse, have been a useful tool in detecting long-term drug exposure. More recently, studies have delved into the exposure of endogenous compounds in hair. Cortisol is synthesized in the adrenal cortex in response to stress-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis. While catecholamines generally indicate acute stress, cortisol can be used as an indicator for sub-acute and chronic stress. Studies on the effects of chronic stress are most often subjective in nature, relying on questionnaires asking the participant to recall on past stressors. This can lead to the issue of recall and reporting bias. A new objective measure of chronic stress is needed for a more accurate understanding of the effects of chronic stress on the body. This review uses emerging evidence to describe the usefulness of hair analysis for cortisol and discusses the current methods used.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"An assessment of cortisol analysis in hair and its clinical applications","attachmentId":66946910,"attachmentType":"pdf","work_url":"https://www.academia.edu/48191846/An_assessment_of_cortisol_analysis_in_hair_and_its_clinical_applications","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/48191846/An_assessment_of_cortisol_analysis_in_hair_and_its_clinical_applications"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="12457883" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/12457883/Detection_of_physiological_concentrations_of_cortisol_and_cortisone_in_human_hair">Detection of physiological concentrations of cortisol and cortisone in human hair</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="31283622" href="https://independent.academia.edu/BertrandLudes">Bertrand Ludes</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinical Biochemistry, 2004</p><p class="ds-related-work--abstract ds2-5-body-sm">Objective: Since the 1960s, glucocorticoids are used by athletes to improve their performances. Their use is restricted in sports. Hair can document chronic abuse and can be therefore a complementary matrix for doping control. We have developed a new extraction, purification, and separation technique using liquid chromatography and mass spectrometry for the identification and quantification of two endogenous glucocorticoids: cortisol and cortisone.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Detection of physiological concentrations of cortisol and cortisone in human hair","attachmentId":46169359,"attachmentType":"pdf","work_url":"https://www.academia.edu/12457883/Detection_of_physiological_concentrations_of_cortisol_and_cortisone_in_human_hair","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/12457883/Detection_of_physiological_concentrations_of_cortisol_and_cortisone_in_human_hair"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="75423279" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/75423279/Assessing_cortisol_from_hair_samples_in_a_large_observational_cohort_The_Whitehall_II_study">Assessing cortisol from hair samples in a large observational cohort: The Whitehall II study</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="59812616" href="https://independent.academia.edu/JaneFerrie">Jane Ferrie</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Psychoneuroendocrinology, 2016</p><p class="ds-related-work--abstract ds2-5-body-sm">Hair cortisol concentrations (HCC) have been suggested to reflect long-term integrated cortisol levels, but most evidence of associations with co-variates is from small samples of healthy volunteers. The objective of this study was to describe the collection of hair samples in a large cohort study and report associations of demographic and health measures with HCC. We examined HCC measured from the 3cm hair segment near the scalp in 3507 participants (aged 59-83y) from The Whitehall II occupational cohort study of British civil servants. Hair samples were analysed using a column switching LC-APCI-MS/MS assay. Findings from mutually adjusted linear regression analyses revealed lower HCC in participants who reported use of hair dye [% difference (95%CI); -12.5 (-22.0, -1.9), p value=0.022] and evidence suggestive of differences by length of sample storage and seasonal variation. With regard to demographic variables, HCC was lower in women compared to men [-17.0 (-24.8, -8.4), p value ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Assessing cortisol from hair samples in a large observational cohort: The Whitehall II study","attachmentId":83198081,"attachmentType":"pdf","work_url":"https://www.academia.edu/75423279/Assessing_cortisol_from_hair_samples_in_a_large_observational_cohort_The_Whitehall_II_study","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/75423279/Assessing_cortisol_from_hair_samples_in_a_large_observational_cohort_The_Whitehall_II_study"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="55421355" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/55421355/Validation_of_an_adapted_procedure_to_collect_hair_for_cortisol_determination_in_adolescents">Validation of an adapted procedure to collect hair for cortisol determination in adolescents</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33122434" href="https://umontreal.academia.edu/IsabelleOuelletmorin">Isabelle Ouellet-morin</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Psychoneuroendocrinology, 2016</p><p class="ds-related-work--abstract ds2-5-body-sm">Introduction: In the last decades, cortisol has been extensively studied in association to early exposure to adversity as well as in the etiology of a number of physical and mental problems. While saliva and blood samples allow the measurement of acute changes in cortisol secretion, hair samples are thought to provide a valid retrospective measure of chronic cortisol secretion over an extended period of time. Nevertheless, the existing protocol for hair collection involves considerable financial and logistical challenges when performed in large epidemiological studies. Objective: This study aimed to validate an adapted collection protocol asking participants to sample their hair at home and to send it back to our laboratory by regular mail. Methods: Participants were 34 teenagers between 17 and 18 years of age. They participated in two hair collections: a) at home, with the help of someone they know, and b) in our laboratory, with a trained research assistant. Results: We noted a strong correlation between cortisol ascertained from hair collected at home and at the laboratory. No mean difference in cortisol levels could be detected between the two protocols. Moreover, we showed that a wide range of hair-related, sociodemographic, lifestyle factors that may be associated with hair cortisol levels did not affect the association between cortisol measures derived from each protocol. Conclusion: Our study provides initial support that reliable measures of chronic cortisol secretion could be obtained by asking adolescents to collect a sample of their hair at home and send them to the laboratory by regular mail. This adapted protocol has considerable financial and logistical advantages in large epidemiological studies.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Validation of an adapted procedure to collect hair for cortisol determination in adolescents","attachmentId":71298694,"attachmentType":"pdf","work_url":"https://www.academia.edu/55421355/Validation_of_an_adapted_procedure_to_collect_hair_for_cortisol_determination_in_adolescents","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/55421355/Validation_of_an_adapted_procedure_to_collect_hair_for_cortisol_determination_in_adolescents"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="100650196" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/100650196/Hair_cortisol_concentrations_in_a_Spanish_sample_of_healthy_adults">Hair cortisol concentrations in a Spanish sample of healthy adults</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="115916370" href="https://independent.academia.edu/AndresGonzalez702">Andres Gonzalez</a></div><p class="ds-related-work--metadata ds2-5-body-xs">PloS one, 2018</p><p class="ds-related-work--abstract ds2-5-body-sm">Hair cortisol concentration (HCC), as a novel promising method to retrospectively measure hypothalamic-pituitary-adrenal (HPA) axis activation, is being increasingly studied. We tested the relationships between HCC and a range of possible confounding variables in a Spanish sample of healthy adults and pregnant women. The number of healthy adults who participated in the study was 529, being 270 males and 259 females, with a combined mean age of 37.88 years (SD = 15.66). Additionally, a separate sample of 62 pregnant women was also recruited with a mean age of 32.95 (SD = 3.67), and in the first trimester of pregnancy. Each participant was interviewed before the study to obtain sociodemographic and lifestyle variables, and a hair sample was taken from the posterior vertex of the head, cut as close to the scalp as possible. Assuming the average growth rate of head hair is 1 cm per month, a 3-cm segment was analysed, in order to measure the cortisol concentrations from a three-month per...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Hair cortisol concentrations in a Spanish sample of healthy adults","attachmentId":101412480,"attachmentType":"pdf","work_url":"https://www.academia.edu/100650196/Hair_cortisol_concentrations_in_a_Spanish_sample_of_healthy_adults","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/100650196/Hair_cortisol_concentrations_in_a_Spanish_sample_of_healthy_adults"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="118793027" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/118793027/Influence_of_external_factors_on_hair_cortisol_concentrations">Influence of external factors on hair cortisol concentrations</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="207120171" href="https://independent.academia.edu/ErichM%C3%B6stl">Erich Möstl</a></div><p class="ds-related-work--metadata ds2-5-body-xs">General and Comparative Endocrinology, 2016</p><p class="ds-related-work--abstract ds2-5-body-sm">Measuring hair cortisol has attracted interest as a long term parameter for chronic stress evaluation. However, some studies support the hypothesis that locally produced cortisol, originating from the hair follicle or skin cells, affects concentrations in the hair. In an animal model the influence of different treatments (extensive brushing, administration of a hyperemising fluid that enhances blood circulation or a synthetic glucocorticoid) on the local cortisol production of hair was evaluated. Therefore eight sheep were sheared and the area of the skin surface of the back was quartered, with three quarters being daily subjected to a certain treatment and one quarter remaining untreated. The skin areas were sheared again after three weeks and cortisol concentrations of all wool samples were determined by immunoassay. Systemic cortisol concentrations were additionally monitored with faecal samples, indicating a significant decline in concentrations of glucocorticoid metabolites between week 1 and 2 or 3, respectively. We found no significant difference in hair cortisol concentrations between fields before treatment (p = 0.310). Comparing matched fields before and after treatment, we found no significant differences in wool cortisol concentrations for fields treated with hyperemising fluid as well as for the control fields (p = 0.329, p = 0.097). Hairs exposed to either extensive brushing or dexamethasone fluid had significantly higher immunoreactive cortisol concentrations after three weeks of treatment (p = 0.016, p = 0.01). We therefore advise cautious interpretation when measuring hair cortisol concentrations as a parameter for chronic stress, because external factors may have a significant influence on the results.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Influence of external factors on hair cortisol concentrations","attachmentId":114335065,"attachmentType":"pdf","work_url":"https://www.academia.edu/118793027/Influence_of_external_factors_on_hair_cortisol_concentrations","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/118793027/Influence_of_external_factors_on_hair_cortisol_concentrations"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":112034826,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":112034826,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_112034826" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="117019606" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/117019606/Hair_Cortisol_Measurement_by_an_Automated_Method">Hair Cortisol Measurement by an Automated Method</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="303965260" href="https://independent.academia.edu/AyelenBalbi">Ayelen Balbi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Scientific Reports, 2019</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link 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data-entity-id="67745438" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/67745438/Title_Validation_of_an_adapted_procedure_to_collect_hair_for_cortisol_determination_in_adolescents_Running_title_Adaption_of_the_collection_protocol_for_hair_cortisol_determination">Title : Validation of an adapted procedure to collect hair for cortisol determination in adolescents Running title : Adaption of the collection protocol for hair cortisol determination</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33122434" href="https://umontreal.academia.edu/IsabelleOuelletmorin">Isabelle Ouellet-morin</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2017</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" 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href="https://independent.academia.edu/JennetteCabibi">Jennette Cabibi</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37919774" href="https://konkuk.academia.edu/JalilGhassemiNejad">Jalil Ghassemi Nejad</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="7944651" href="https://konkuk.academia.edu/JGhassemiNejad">Jalil Ghassemi Nejad</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"A Cortisol Study; Facial Hair and Nails","attachmentId":49584779,"attachmentType":"pdf","work_url":"https://www.academia.edu/29138013/A_Cortisol_Study_Facial_Hair_and_Nails","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download 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