Multiplex Assays and Multiplex Immunoassays

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Mouse XL Cytokine Assay Panel Available Now</h2></div> <a href="/p/luminex-assays/mouse-xl-cytokine-luminex-performance-premixed-kit_fcstm20" > <button type="button" class="btn btn-primary"> Buy Now </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Multiplex Assays for Accurate, Consistent Analysis</h2> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/luminex-assays" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/teaser-trim-xmap-iflex-openside-700x420.png" alt="Luminex Intelliflex xMAP instrument with drawer open" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/reagents/luminex-assays" > <h3 class="card_title_htag"> <div>Need Maximum Analyte Flexibility and Selection?</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Profile and validate your biomarker candidates with Luminex<sup>®</sup> Discovery and Luminex High Performance multiplex immunoassays.   </p> <ul> <li>Multiplex up to 50 analytes per sample.</li> <li>Choose from more than 450 analytes in 3 flexible formats.</li> <li>Customize panels to fit your needs.</li> <li>Rigorously validated for accuracy, reproducibility, and lot-to-lot consistency.</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/luminex-assays" > Explore Luminex Assays </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-plex" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/ella-front-feature1.png" alt="Automated ELISA system Ella from ProteinSimple runs high throughput multiplex assays" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/instruments/simple-plex" > <h3 class="card_title_htag"> <div>Need High Throughput and Fast Validation?</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Bring a higher level of precision and automation to your immunoassay workflow with Simple Plex™ assays on the Ella™ platform.</p> <ul> <li>Get consistent, reproducible results in less than 90 minutes.</li> <li>Generate triplicate data with only 25 µL of sample volume.</li> <li>Reduce human error and variability with hand-free automation.</li> <li>Choose from a menu of more than 250 fully validated assays.</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-plex" > View Simple Plex Assay Options </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--header"> <div> </div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/IA-workflow-1110x616.png" alt="Multiplex assay options including multiplex western system, multiplex arrays and automated multiplex immunoassays " loading="lazy" > </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p> </p> <h2>Types of Multiplex Assays</h2> <p> </p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/proteome-sized.png" alt="Proteome Profiler antibody arrays" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div>Screening/Exploration</div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h4>Antibody Arrays</h4> <p>Providing broad, cost-effective early-stage screening, antibody arrays offer a quick and inexpensive analysis of many analytes simultaneously—in less time than it takes to perform a Western blot.   </p> <p>Similar to an ELISA but with expanded multiplexing capabilities, these semiqualitative antibody arrays can analyze hundreds of different proteins simultaneously with substantially less time and effort.  Rather than a plate, antibody arrays use a membrane as a substrate. Capture antibodies are pre-spotted in duplicate with high-quality capture antibodies for multiplexing.  </p> <p>With superior specificity, low background noise, and no cross-reactivity, you can count on this assay for clear and consistent data. </p> <ul> <li>Fast, economical analysis</li> <li>No specialized equipment needed</li> <li>Measure more than 100 analytes at once</li> <li>Quickly analyze complex signaling responses.</li> </ul></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/reagents/proteome-profiler-antibody-arrays" class="btn btn-primary" type="link"> Learn More About Antibody Arrays </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/webinars/webinar-luminex-thumbnail-525x315.jpg" alt="Luminex Multiplex Assays " loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div>Precise, Accurate Biomarker Discovery</div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h4>Luminex Multiplex Assays</h4> <p>Luminex multiplex assays are designed to maximize multiplexing capacity and flexibility while maintaining assay specificity. The bead-based Luminex format allows you to better tailor assay selection to your individual research needs while overcoming many common multiplexing challenges. </p> <p>Utilizing the same sandwich principles of traditional ELISAs, color-coded microspheres, or beads, are internally dyed with different proportions of red and infrared fluorophores that correspond to a distinct spectral signature, or bead region. Antibodies specific to the desired analyte are coupled to a unique bead region and are incubated with the sample.</p> <p>Luminex multiplex assays allow you to quantify up to 50 biomarkers with less sample volume than a traditional plate-based ELISA.</p> <ul> <li>Accurate bead-based assays</li> <li>Multiplex up to 50 analytes simultaneously </li> <li>Customize panels to fit your needs</li> <li>Rigorously validated for accuracy, reproducibility, and lot-to-lot consistency</li> </ul></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/reagents/luminex-assays" class="btn btn-primary" type="link"> Explore Luminex Multiplex Assays </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/simple-plex-automated-immunoassays-banner-solo-2.png" alt="Automated ELISA systems Ella runs single, multianalyte and multiplex immunoassays" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div>Fully Automated ELISA</div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><h4>Cartridge-Based Immunoassays</h4> <p>With multi-analyte cartridge-based assays, you get the same specificity of a single-analyte ELISA but with greater sensitivity and a broader dynamic range. The automated immunoassay cartridges deliver results in less than 90 minutes with better sensitivity and reproducibility than plate-based assays.  </p> <p>Each cartridge accommodates up to 16, 32, or 72 samples, and each well is sampled three times to give you all the results in triplicate. The antibodies as well as the fluorescent detector are pre-functionalized in the cartridge, eliminating the need to run a standard curve.</p> <p>Disposable cartridge kits come in a variety of multi-analyte and single-analyte configurations and include everything you need to run your automated ELISA. The reduction in manual steps enables a high level of reproducibility and data quality—all with a method that's fast and easy to transfer. </p> <ul> <li>Highly sensitive results in less than 90 minutes</li> <li>Choose from over 250 validated targets</li> <li>Hands-free automation with high-throughput</li> <li>Available in single and multi-analyte cartridge formats</li> </ul></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/instruments/simple-plex" class="btn btn-primary" type="link"> Meet the Next Generation ELISA </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>The Latest in Multiplexing Technology</h2> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/luminex-assays/mouse-xl-cytokine-luminex-performance-premixed-kit_fcstm20" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/teaser-mousexl700x420-min.png" alt="Luminex Mouse Cytokine Panel" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/luminex-assays/mouse-xl-cytokine-luminex-performance-premixed-kit_fcstm20" > <h3 class="card_title_htag"> <div>Mouse XL Cytokine Luminex Assay Panel</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Flexible, premixed Metabolic Luminex panels can be configured to quantify up to 45 biomarkers per 50 uL sample in less than a day, helping to save time and precious samples while accelerating your workflow. Rigorous quality control processes help eliminate false-positive data. </p> <ul> <li>The largest customizable mouse Luminex assay on the market providing you with 45 biomarkers</li> <li>The panel is available in multiple format options including premix, user mix, and fixed </li> <li>7 exclusive targets: EGF, FGF-basic, Flt-3 Ligand, GDF-15, IL-1ra, IL-11 & LDL R</li> </ul> <p> </p></div> </div> <div class="card-bottom-link"> <div> <a href="/p/luminex-assays/mouse-xl-cytokine-luminex-performance-premixed-kit_fcstm20" > Customize Your Luminex Mouse XL Cytokine Assay Panel </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/simple-plex-immunoassays" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/images/webinar/LP_images/cgt-gmp-capabilities-expect-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/ella-cartridge-32x8-hero-front-480x480-2.png" alt="Simple plex cartridge 32x8" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/simple-plex-immunoassays" > <h3 class="card_title_htag"> <div>Multiplex Assays on Ella</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Simple Plex multiplex assays combined with Ella's speed and automated workflow allow you to create large datasets quickly with minimal manual intervention. The 32 sample multiplex cartridge offers your choice of either 5, 6, 7, or 8 immunoassays in duplicate from just 25 µL of sample.  </p> <ul> <li>Reduce setup time with factory-calibrated standard curves</li> <li>Get the same assay results no matter the cartridge format  </li> <li>Maximize throughput with up to 256 reportable results in less than 90 minutes</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/simple-plex-immunoassays" > Explore Simple Plex Assay Cartridges </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-western" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/webinars/webinar-thumbnail-best-practices-for-multiplex-western-blots.jpg" alt="Webinar with tips for Utilizing RePlex Sequential Immunoassays and Total Protein Normalization on Simple Western" loading="lazy" > </div> </a> <a class="card_title_link" href="/instruments/simple-western" > <h3 class="card_title_htag"> <div>RePlex on Simple Western</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>With the RePlex™ features on Jess™ and Abby™, you can run two immunoassays within the same capillary to get all your rich protein characterization data from just one sample.</p> <ul> <li>Quantify expressed phosphorylated target and total target levels</li> <li>Normalize your data with total protein expression data in the same capillary</li> <li>Gain greater immunoprobe selection flexibility</li> <li>Get more data points per sample</li> <li>Save time and money on consumables</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-western" > Learn More About RePlex on Simple Western </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/rnascope-ish-technology" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/therapeutic-manufacturing/rnascope-multiplex-fluorescence-1.jpg" alt="Detection of anti-BCMA CAR-T cells, Inteferon-g expression by RNAscope multiplex fluorescence, and CD3+ T cells by immunofluorescence" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/rnascope-ish-technology" > <h3 class="card_title_htag"> <div>RNAscope™ Multiplex Fluorescent V2 Assay</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>The RNAscope Multiplex Fluorescent v2 assay provides exceptional sensitivity while allowing simultaneous detection of up to four RNA targets within a single sample using up to four target probes.</p> <ul> <li>Nuclei can be counter-stained and visualized using DAPI fluorescent dye </li> <li>Sequential assay workflow provides flexibility to visualize any probe in any of the TSA-linked fluorophore channels.</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/rnascope-ish-technology" > Explore RNAscope Fluorescent Multiplex Assays </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Multiplex Immunoassay Applications</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/research-areas/immunology" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/images/webinar/LP_images/Protein-Bioprocessing-LP-Discovery-Immune-Cell.png" alt="Bio-techne can help you fast-track your discovery stages with automated and innovative solutions." loading="lazy" > </div> </a> <a class="card_title_link" href="/research-areas/immunology" > <h3 class="card_title_htag"> <div>Immunology</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Investigate and quantify immune responses using our wide range of multiplex assays and automated platforms that are specifically designed to simplify experiments and accelerate results.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/research-areas/immunology" > Explore Analytical Solutions for Immunology </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/research-areas/cell-and-gene-therapy" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dw-latest-news-img5_stry0134948.png" alt="iPSC Therapies – Cell and Gene Therapy Symposia Series" loading="lazy" > </div> </a> <a class="card_title_link" href="/research-areas/cell-and-gene-therapy" > <h3 class="card_title_htag"> <div>Cell and Gene Therapy</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Rigorously characterize your mid-process and final cell products. Get your therapies to patients quickly and safely using our highly reliable and reproducible assays and analytical platforms. </p></div> </div> <div class="card-bottom-link"> <div> <a href="/research-areas/cell-and-gene-therapy" > Automated Assays for Cell and Gene Therapy </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/research-areas/cancer-profiling" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/teaser-cancerio-app-2.jpg" alt="Cancer & Immuno-Oncology" loading="lazy" > </div> </a> <a class="card_title_link" href="/research-areas/cancer-profiling" > <h3 class="card_title_htag"> <div>Cancer Research</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Identify and track your cancer biomarker candidates using our gold-standard analytical instruments and tools. Gain the superior throughput and high-quality data you need for reliable biomarker validation.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/research-areas/cancer-profiling" > Instruments and Tools for Cancer Biomarker Discovery </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Other Multiplex Assay Options</h2> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-western/multiplex-protein-analysis-simple-western" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/applications/bg-halfcol-blue-standard-curve-1080x400.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/fore-halfcol-instrument-603x422.png" alt="Jess and Peggy Sue instruments" loading="lazy" > </div> </a> <a class="card_title_link" href="/instruments/simple-western/multiplex-protein-analysis-simple-western" > <h3 class="card_title_htag"> <div>Gel-Free, Blot-Free Westerns</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>Capillary-Based Assays</strong></p> <p>With the capillary-based Simple Western™ platform, you can detect multiple proteins in the same capillary using two or more antibodies, enabling a variety of strategies for the detection of multiple proteins in each sample.  With Simple Western, you get rich and quantitative multiplexed protein characterization data with just 3 µL of sample, and it’s fully automated so you can design your next experiment while Simple Western does the work for you.</p> <p><strong>Single-Cell Analysis</strong></p> <p><strong><a href="/instruments/single-cell-western">Single-Cell Western</a></strong> on Milo measures protein expression in ~1000 single cells so you can quantify heterogeneity of your target at the single-cell level and detect up to 12 targets per cell with a variety of multiplex strategies.</p> <p><strong>Find out more about Simple Westerns.</strong></p></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-western/multiplex-protein-analysis-simple-western" > Learn More About Multiplex Protein Analysis </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/rnascope-ish-technology" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/fore-halfcol-magnifyb-603x422.png" alt="highly sensitive molecular detection" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/reagents/rnascope-ish-technology" > <h3 class="card_title_htag"> <div>Highly Sensitive Molecular Detection</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p><strong>RNA-ISH Assays</strong></p> <p>Recent advances in RNA in situ hybridization (ISH) assay technology provide exceptional sensitivity, allowing single-molecule detection of RNA targets at the single-cell level. RNA-ISH assays are ideal for simultaneous detection studies of any genes in nearly any tissue type using fluorescent labels. </p> <p>The assays are available with various levels of plexing, allowing you to:</p> <ul> <li>Plex RNA and protein targets on the same slide for multi-omic analysis in situ’</li> <li>Find cells of interest by using cell-specific markers</li> <li>Confirm cells by simultaneous detection of additional targets</li> <li>Characterize cellular heterogeneity of complex tissues  </li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/rnascope-ish-technology" > Learn About RNAscope ISH Technology </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Raising the Bar in Multiplex Performance</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://www.rndsystems.com/quality/product-development" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/fore-thirdcol-labtechb-700x420.png" alt="Deep multiplexing expertise" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://www.rndsystems.com/quality/product-development" > <h3 class="card_title_htag"> <div>Deep Multiplexing Expertise</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Multiplexing calls for carefully chosen compromises that allow analytes to play well together while still producing accurate, meaningful results. Bio-Techne has deep and broad-based experience developing and manufacturing immunoassays. Extensive technical expertise and rigid quality control help ensure we build the most reliable assays with superior precision, accuracy, sensitivity, and reproducibility.  </p></div> </div> <div class="card-bottom-link"> <div> <a href="https://www.rndsystems.com/quality/product-development" > Expert Assay Development at R&D Systems </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://www.rndsystems.com/quality/performance" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/fore-thirdcol-histogram-700x420.png" alt="Unparalleled Assay Quality" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://www.rndsystems.com/quality/performance" > <h3 class="card_title_htag"> <div>Unparalleled Assay Quality</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Confidence in multiplex immunoassay data hinges on superior accuracy and precision that is only possible from using high-quality raw materials. Bio-Techne antibodies, proteins, and specialized diluents are the foundation of all our immunoassays.  All of our assays and complete kits go through stringent quality control testing to make sure they remain consistent, and your data is reproducible.  </p></div> </div> <div class="card-bottom-link"> <div> <a href="https://www.rndsystems.com/quality/performance" > Built on a Foundation of Quality at R&D Systems </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/services/custom-formulation" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/fore-thirdcol-antibody-700x420.png" alt="Antibody for Custom Assay Solutions" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/services/custom-formulation" > <h3 class="card_title_htag"> <div>Custom Assay Solutions</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Looking for a unique analyte or multiplex panel? Bio-Techne experts will work closely with you to produce multiplex assays that are tailored to fit your experimental needs. Custom assay services include new assay development, qualification, assay modification, and species testing.<strong> </strong>When your needs change, you can move seamlessly between platforms with consistency in your results.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/services/custom-formulation" > Custom Assays and Formulations </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/luminex-525x525.jpg" alt="Luminex bead based multiplex assays" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h3>What are the Advantages of Multiplex Assays?</h3></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Growing demand for immunoassays and surging testing volumes has put pressure on scientists to rein in costs and optimize sample usage by designing multiplex assays to cover several analytes simultaneously. Factor in the time saved by running the equivalent of 20 or more different assays in a single immunoassay workflow, and the benefits of multiplexing are clear.</p> <p>The ability to measure multiple targets in a single assay allows researchers to detect hundreds of analytes in a single run while being judicious with precious sample volumes. From screening biomarkers for further evaluation to validating results for drug development, multiplex assays provide several important benefits, helping to:</p> <ul> <li>Conserve limited sample volume</li> <li>Lower your cost per data point</li> <li>Improve accuracy due to less sample handling</li> <li>Optimize productivity</li> <li>Save time and resources</li> </ul></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h3>How Can Automation Help?<h3></h3></h3></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Some of the latest developments in multiplexing include new automated platforms designed to eliminate user error and accelerate time-to-results. Automation not only improves the efficiency of critical steps along the discovery continuum but also helps remove many potential sources of variability to provide more accurate and reproducible results.</p> <p><a href="/instruments/simple-plex"><strong>Simple Plex</strong></a>™ assays running on the Ella™ platform are a good example, With Simple Plex, you get the same reproducible answers across multiple users and multiple sites, regardless of the user. The assay works like a traditional ELISA except everything is pre-loaded into the cartridge. You simply dilute your sample and insert the cartridge into Ella. Each assay is run the same way every time—with no manual steps, washes, or reagent additions. </p> <p>While different labs require different degrees of automation, the underlying need for greater precision, throughput, and reproducibility remains a core driver across research sectors.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/p/simple-plex/ella-automated-immunoassay-system_600-100" class="btn btn-primary" type="link"> Meet Your Next-Generation ELISA </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/immunoassay-brian-astry-scientist-525x315.png" alt="Scientist Brian Astry does immunoassay research" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="key-features"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/immunoassay-mandy-kubik-scientist-525x315.png" alt="Scientist Mandy Kubik does immunoassay research" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h3>What Are the Key Features of the Best Multiplex Immunoassays?</h3></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>Confidence in immunoassay data depends on the accuracy and precision that is only possible from using high-quality <strong><a href="/reagents/antibodies">antibody reagents</a></strong>. This means selecting antibodies with not only high analyte affinity but also specificity, which is the ability to distinguish between the analyte and other structurally similar components.</p> <p>While multiplexing offers numerous efficiency advantages, it also introduces several technical challenges that make assay design more complicated. One of the biggest challenges is controlling for cross-reactivity and interference between all the antibodies and proteins in the assay. Do you get the same results from IL-6 when run alone and when with 20 other analytes?</p> <p>Single-analyte assays are designed to be analyte-specific—meaning that every component, from buffer to antibody pairs and more, are custom-tailored for that specific analyte. Assays incorporating multiple analytes do not have this luxury.</p> <p><strong><a href="/reagents/luminex-assays/luminex-guide" target="_self">Multiplexing</a></strong> requires carefully integrated balances that allow analytes to work consistently together to produce consistent, accurate results. Interfering factors, no matter the mechanism, can result in either reduced or elevated signals relative to the actual concentration of target analyte.</p> <p>While validation protocols help ensure that antibodies are producing appropriate results, if the assay isn’t carefully developed and properly designed, achieving optimal performance will be difficult.</p> <p>At Bio-Techne, we take great care to select the antibodies and proteins that can be plexed harmoniously to reduce interference and maximize performance across all analytes. Meanwhile, our rigorous quality control and validation efforts confirm the performance meets required standards and specifications lot after lot.</p></div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h3>Ensuring Assay Precision and Accuracy</h3></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>One challenge in multiplex assay design is creating a buffer system that is optimized to perform well with other analytes in the panel. Since it is not possible to customize the buffer to any individual analyte, optimizing buffers for peak performance, such as linearity or recovery, and managing matrix effects are key steps to ensuring sensitivity, precision, and accuracy of the resulting immunoassay.</p> <p>There are primarily two reasons for dilutions. In some assays most samples read above the standard curve, thus requiring a dilution for analyte levels to fall within the range of the assay. A second reason for dilution is to limit interference due to factors in complex matrices.</p> <p>In the best scenario, an assay diluent works well with a broad range of analytes, allowing scientists to multiplex anywhere from a few to several dozen analytes within a single test. To avoid incorrect dilutions in multiplex immunoassays, you need to know the relative abundance of the analytes to be tested.</p> <p>Higher-abundance analytes may require a different sample dilution than those of lower abundance. Grouping the analytes by common dilution is the most efficient means of creating multiplex panels.</p> <p>No matter what dilution samples are run at, you should always derive the same concentration once back calculated, giving you consistency in results. As you dilute the sample, the interfering factors also dilute out, making the target analyte more accessible, and the concentration of the target analyte increases.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/immunoassay-valeriu-bortnov-scientist-525x315.png" alt="Scientist Valeriu Bortnov does immunoassay research" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/luminex-high-performance-assay-card.png" alt="Luminex High Performance Assay - Icon" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h3>Multiplex with Speed and Efficiency</h3></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p>While developing a multiplex assay presents several complexities compared to single analyte, plate-based ELISAs, the ability to generate high-quality data while dramatically reducing time and costs needed for testing provides researchers with a powerful advantage. From narrowing down drug candidates in preclinical studies to evaluating therapeutic responses during a clinical trial, multiplex assays provide several important benefits, including the ability to:</p> <ul> <li><strong>Maximize limited test samples</strong>: collect data from just 25μl or less of undiluted sample.</li> <li><strong>Minimize experimental variability</strong>: derive multiple data points from a single manipulation.</li> <li><strong>Optimize productivity</strong>: reduce sample preparation and processing time.</li> <li><strong>Improve efficiency</strong>: examine multiple analytes in a single sample.</li> </ul> <p>Meeting immunoassay performance goals is an ongoing challenge across research fields. The good news is that today’s scientists have access to an abundance of external experts who have deep technical experience and access to vast libraries of <a href="/reagents/antibodies">antibodies</a> to help develop their assays. Engaging with an experienced supplier like Bio-Techne and paying attention to the technical factors outlined here will significantly improve your chances of developing high-quality, highly effective multiplex assays.</p></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_call_to_action_global"> <a href="/applications/immunoassays" class="btn btn-primary" type="link"> Explore Bio-Techne Immunoassay Options </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3><strong>Additional Immunoassay Tips and Insights</strong></h3> <ul> <li><strong><a href="/applications/immunoassays/important-plate-based-immunoassay-features" target="_self">What is the most important feature of any plate-based immunoassay?</a></strong></li> <li><strong><a href="/applications/immunoassays/which-immunoassay-will-meet-my-research-needs" target="_self">Which immunoassay will best meet my research needs?</a></strong></li> <li><strong><a href="/applications/immunoassays/immunoassay-expert-advice" target="_self">What are some other ways to improve immunoassay performance?</a></strong></li> </ul></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-additional-resources24" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Additional Resources</h5> </a> </div> <div class="collapse" id="expPara-additional-resources24" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><ul> <li><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/an_simple_western_single-cell_western_immuno-oncology.pdf" target="_blank"><strong>Automated Western Assays to Develop New Immunotherapies</strong></a></li> <li><a href="https://youtu.be/tQ27pt_PmhU" target="_blank"><strong>Learn How to Design and Run a Luminex Assay</strong></a></li> <li><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/AN_Fast-and-Reproducible-AAV2_STRY0156312.pdf" target="_blank"><strong>Fast and Reproducible AAV2 Vector Quantification</strong></a></li> <li><a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/cell-kill-appnote.pdf" target="_blank"><strong>Quantify Secreted T Cell Activation Markers in 90 Minutes</strong></a></li> <li><a href="https://www.youtube.com/watch?v=1lt1f_-EIwg" target="_blank"><strong>Learn How to Use the Proteome Profiler Antibody Array</strong></a></li> <li><a href="/reagents/rnascope-ish-technology/get-started"><strong>Getting Started with RNAscope ISH Technology</strong></a></li> <li><a href="https://www.rndsystems.com/promotions/luminex-assay-users-guide" target="_blank"><strong>Luminex Assay User Guide</strong></a></li> <li><strong><a href="https://www.rndsystems.com/resources/literature/immunoassay-solutions-neuroinflammation" target="_blank">Immunoassay Solutions for Neuroinflammation</a></strong></li> <li><a href="/resources/simple-western-antibody-database"><strong>Simple Western Antibody Database</strong></a></li> <li><a href="/resources/single-cell-western-antibody-database"><strong>Milo Antibody Database</strong></a></li> </ul></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p>Luminex® is a Registered Trademark of Luminex Corporation.</p></div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> <div class="footer-logo col-xl-3 col-lg-auto d-flex"> <img class="brand-bar-footer-logo" src="/themes/custom/bio_techne_global/logo.svg" alt="Bio-techne logo"> </div> <div 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