Explore Organoid and 3-D Cell Culture Products

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url('https://resources.bio-techne.com/bio-techne-assets/images/research-areas/organoid-3-d-culture-products-hero-v2.jpg'); background-position: top center; background-repeat: no-repeat; background-size: cover;"> <div> <div class="paragraph paragraph--type--h1-2-2-text-to-media paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media:field_indv_h1"> <div><h1>Organoid and 3D Cell Culture Products</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Bio-Techne offers a complete range of products designed to promote robust organoid growth, while maintaining consistent, reproducible culture conditions. We provide basement membrane extracts, including our <strong>NEW</strong> Cultrex™ UltiMatrix RGF Basement Membrane Extract, along with growth factors, small molecules, media, and media supplements. Additionally, we offer antibodies and RNAscope™ <em>in situ</em> hybridization assays for identifying lineage-specific markers.</p></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media:field_call_to_action_global"> <a href="#tools" class="btn btn-primary" type="link"> Explore Our Products </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> </div> </div> </div> </div> </div> </div> </div> </section> </div> </div> </header> </div> <div id="main" class="container-fluid container-wide"> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/research-areas">Research Areas</a> </li> <li> Organoid and 3D Cell Culture Products </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--clickable-icons-container paragraph--view-mode--default"> <div class="container"> <div class="row"> <div class="col p-0 "> <h2></h2> </div> </div> <div class="row"> <div class="col "> </div> </div> <div class="row"> <div class="row justify-content-start"> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#what" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/organoid-light-blue-icon.png' alt='icon of an organoid'></div> </article> </div> <p><strong>Introduction: What are Organoids</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#tools" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/tools-organoid-icon.png' alt='icon of a computer monitor with a pencil in front of it'></div> </article> </div> <p><strong>Tools for Organoid Culture</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#protocols" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/organoid-protocols-icon.png' alt=' icon of an Erlenmeyer flask and beaker'></div> </article> </div> <p><strong>Organoid Protocols</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#organoid-data" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/organoid-data-icon.png' alt='icon of an organoid under a magnifying glass'></div> </article> </div> <p><strong>Organoid Data</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#literature" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/literature-organoid-icon.png' alt=' icon of open book'></div> </article> </div> <p><strong>Literature for Organoid Research</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="#additional" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/additional-resources-icon.png' alt='icon of two gears'></div> </article> </div> <p><strong>Additional Resources</strong></p> </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="what"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>What Are Organoids?</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>An organoid is a miniaturized version of an organ that can be generated <em>in vitro </em>under specific culture conditions from primary tissue, embryonic <a href="/research-areas/stem-cells">stem cells</a> (ESCs), or induced pluripotent stem cells (iPSCs). Organoids have similar cellular composition and architecture as their tissues of origin and exhibit functional similarities. They are typically composed of multiple different cell types, which are properly arranged with respect to both each other and the extracellular matrix. Organoids are not only capable of self-organization, they are also capable of self-renewal, and are amenable to long-term cultivation and manipulation. Researchers have developed organoid systems for many different human tissues, including intestine, stomach, colon, liver, lung, heart, pancreas, kidney, prostate, inner ear, brain, and heart, among others. These 3D <em>in vitro</em> model systems have opened new avenues for investigating organ development, studying physiology and disease, screening drugs, and exploring personalized medicine. Bio-Techne offers a wide range of products aimed at promoting robust, reproducible organoid growth. From basement membrane extracts to growth factors and small molecules, we have the reagents that you need for establishing optimal organoid culture conditions, with minimal variability from one experiment to the next.   </p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/2x2-organoid-man-v2.jpg" alt="Organoid and 3-D Cell Culture Products Teaser BG" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="tools"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Tools for Organoid Culture</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cultrex-basement-extract-350x210-v2.jpg" alt="organoid cultured in Cultrex UltiMatrix RGF Basement Membrane Extract" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins" > <h3 class="card_title_htag"> <div><h3>Cultrex Extracellular Matrices</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>• Different formats of organoid-qualified Cultrex Basement Membrane Extracts are available to promote robust cell growth, viability, and culture consistency</p><p>• Our latest, most advanced <a href="/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05">Cultrex UltiMatrix RGF Basement Membrane Extract</a> has been optimized to improve the performance and consistency of spheroid, organoid, and stem cell cultures</p><p>• Reduced growth factor formulations provide more defined culture systems</p><p>• Quality controlled for lot-to-lot performance consistency</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins" > Explore Our Selection of Cultrex Extracellular Matrices </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/proteins/organoid-culture-proteins" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/recombinant-proteins-525x315.jpg" alt="Representative ribbon structure of a recombinant protein" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/proteins/organoid-culture-proteins" > <h3 class="card_title_htag"> <div><h3>Recombinant Proteins for Organoid Culture</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>• We offer an extensive catalog of proteins commonly used for organoid culture, including proteins that are difficult to express and purify such as <a href="/t/r-spondin-1/proteins-enzymes">R-Spondin-1</a>, <a href="/t/noggin/proteins-enzymes">Noggin</a>, and <a href="/t/wnt-3a/proteins-enzymes">Wnt-3a</a></p><p>• All of our recombinant proteins are rigorously tested to ensure high levels of bioactivity and lot-to-lot consistency, and low endotoxin levels</p><p>• Our proteins are highly cited in organoid-related scientific publications </p><p>• From basic research to preclinical and clinical applications, we offer research-grade, <a href="/reagents/proteins/animal-free-proteins">Animal-free RUO</a>, and <a href="/gmp-products/gmp-proteins">Animal-free GMP-grade</a> cytokines to meet every researcher's need</p><p>• Our recombinant proteins can be ordered in <a href="/services/bulk-quotes">bulk</a> at discounted prices and custom protein services are also available</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/proteins/organoid-culture-proteins" > Browse Proteins for Organoid Culture </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/small-molecules-and-peptides/organoids-small-molecules" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/small-molecule-525x315.jpg" alt="Representative image of a small molecule" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/small-molecules-and-peptides/organoids-small-molecules" > <h3 class="card_title_htag"> <div><h3>Small Molecules for Organoids</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>• A large selection of small molecules that are commonly used as 3-D growth matrix components and additives for long-term organoid growth are available</p><p>• Our catalog includes the ALK kinase inhibitor, <a href="/p/small-molecules-peptides/a-83-01_2939">A 83-01</a>, the highly selective GSK-3 inhibitor <a href="/p/small-molecules-peptides/chir-99021_4423">CHIR 99021</a>, the potent ROCK inhibitor, <a href="/p/small-molecules-peptides/y-27632-dihydrochloride_1254">Y-27632 dihydrochloride</a>, the p38 MAPK inhibitor, <a href="/p/small-molecules-peptides/sb-202190_1264">SB 202190</a>, and many more</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/small-molecules-and-peptides/organoids-small-molecules" > View Small Molecules for Organoid Culture </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=%22cell%20culture%20media%22%20OR%20%22media%20supplements%22" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/mouse-colon-organoids-290x200.jpg" alt="Mouse colon organoids grown in Cultrex RGF BME stained for E-Cadherin and counterstained with DAPI" loading="lazy" > </div> </a> <a class="card_title_link" href="/search?keywords=%22cell%20culture%20media%22%20OR%20%22media%20supplements%22" > <h3 class="card_title_htag"> <div><h3>Media and Media Supplements</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>• We offer a variety of different formats of base media for organoid cultures, including media with or without glucose, L-glutamine, HEPES, NEAA, nucleosides, phenol red, or sodium pyruvate content</p><p>• We develop and manufacture <a href="/search?keywords=media%20supplements&sub_category=Media%20Supplements">serum-free media supplements</a> to support organoid cultures</p><p>• Our catalog includes <a href="/search?keywords=media%20supplements&sub_category=Cryopreservation%20Media">cryopreservation media</a>, which are optimized to increase cell recovery and viability</p><p>• Our New <a href="/p/cell-culture/excellerate-ipsc-expansion-medium-animal-free-gmp_ccm036-gmp">ExCellerate GMP iPSC Expansion Medium</a> is completely animal-free and does not require the addition of growth factors</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=%22cell%20culture%20media%22%20OR%20%22media%20supplements%22" > Browse Our Complete Selection of Media and Media Supplements </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/antibodies" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/super-resolutio-microscopy-antibodies-teaser.jpg" alt="Super Resolution Antibodies Teaser" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/antibodies" > <h3 class="card_title_htag"> <div><h3>Antibodies for Detecting Lineage-Specific Markers</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>• Our catalog includes over 425,000 R&D Systems™ and Novus Biologicals™ antibodies for more than 25 species</p><p>• We offer both unlabeled and labeled primary antibodies with 20 different potential fluorescent conjugates</p><p>• Our primary antibodies are validated for one or more of up to 15 applications</p><p>• We offer both conjugated and unconjugated secondary antibodies and a range of isotype control antibodies</p><p>• Our antibodies come in a variety of size options, including <a href="/services/bulk-quotes">bulk</a> and sample sizes to ensure that you can find what you need for your research</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/antibodies" > Learn More About Our Antibodies </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/rnascope-ish-technology" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/images/research/neuro-visualize-acd-bio-rnascope-multiplex-fluorescent-assay.jpg" alt="Quantitative Molecular Detection with Morphological Context and In Situ Hybridization (ISH)" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/rnascope-ish-technology" > <h3 class="card_title_htag"> <div><h3>RNAscope <em>In Situ</em> Hybridization Assays</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>• Utilize this technology to spatially map and quantify specific target mRNAs in intact cells and tissues</p><p>• The assay can be combined with immunohistochemistry or immunofluorescence on the same slide to investigate mRNA and protein co-expression</p><p>• Up to 12 RNA targets can be detected simultaneously using the RNAscope HiPlex assay</p><p>• Catalog probes are available for over 23,000 targets in 140+ species and can also be made-to-order</p><p>• RNAscope ISH Assays are cited in over 2,600 publications across multiple different research areas</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/rnascope-ish-technology" > Learn More About RNAscope ISH Technology </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="protocols"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Organoid Protocols</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--clickable-icons-container paragraph--view-mode--default"> <div class="container"> <div class="row"> <div class="col p-0 text-left"> <h2></h2> </div> </div> <div class="row"> <div class="col text-left"> </div> </div> <div class="row"> <div class="row"> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center h-100 mb-0"> <a href="/resources/protocols-troubleshooting/human-intestinal-organoid-culture" > <div><article> <div><img src='https://resources.bio-techne.com/bio-techne-assets/images/icons/intestinal-organoid-protocol-icon.png' alt='Icon of an intestine organ'></div> </article> </div> <p><strong>Intestinal Organoid Protocol</strong></p> </a> </div> </div> <div class="col-xl-2 col-lg-2 col-md-4 col-sm-6 col-6 mb-4"> <div class="paragraph paragraph--type--indvidual-clickable-icon paragraph--view-mode--default card p-3 text-center 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<div> <div><h2>Learn Why Scientists Choose Our Products for their Research</h2></div> <a href="/research-areas/organoids-3d-culture/peer-product-reviews" > <button type="button" class="btn btn-primary"> Read Scientists' Comments </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="organoid-data"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Organoid Data</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong>Human Lung Organoids Grown in Cultrex UltiMatrix RGF Basement Membrane Extract.</strong> Adult stem cells isolated from human lung biopsy tissue were embedded in <a href="/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05">Cultrex UltiMatrix RGF Basement Membrane Extract</a> (R&D Systems, Catalog # BME001-05) and cultured in media for 20-60 days. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. A) Lung organoids were stained with anti-Cytokeratin 5 (KRT5) (green; Novus Biologicals) and with <a href="/p/antibodies/human-p63-tp73l-antibody_af1916">anti-p63/TP73L</a> (red; R&D Systems, Catalog # AF1916) to visualize basal cells. B) Lung organoids were stained with <a href="/p/antibodies/podoplanin-antibody-811_nb600-1015" target="_blank">anti-Podoplanin</a> (PDPN) (green; Novus Biologicals, Catalog # NB600-1015) to visualize alveolar type I cells. C, D) Lung organoids were stained with <a href="/p/antibodies/muc5ac-antibody-45m1_nbp2-15196" target="_blank">anti-Muc5ac</a> (green; Novus Biologicals, Catalog # NBP2-15196) to visualize Goblet cells and with <a href="/p/antibodies/human-mouse-rat-sox2-antibody-245610_mab2018">anti-Sox2</a> (red; R&D Systems, Catalog # MAB2018). All samples were counterstained with the nuclear stain <a href="/p/fluorescent-probes-dyes/dapi_5748">DAPI</a> (blue; Tocris, Catalog # 5748).</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/products/human-lung-organoids-v5.jpg" alt="Human lung organoids grown in Cultrex UltiMatrix RGF BME and stained for Cytokeratin 5, p63, Podoplanin, Muc5a, and SOX2." loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong>Human Descending Colon Organoids.</strong> Adult stem cells isolated from human descending colon were embedded in <a href="/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05">Cultrex UltiMatrix RGF Basement Membrane Extract</a> (R&D Systems, Catalog # BME001-05) and cultured in growth medium for 30 days prior to imaging. A, B) Descending colon organoids were stained with <a href="/p/antibodies/human-chromogranin-a-antibody-948128_mab90981">Chromogranin A</a> (green; R&D Systems, Catalog # MAB90981) to visualize intestinal enteroendocrine cells, and counterstained for <a href="/p/antibodies/human-mouse-e-cadherin-antibody_af748">E-Cadherin</a> (red; R&D Systems, Catalog # AF748) and <a href="/p/fluorescent-probes-dyes/dapi_5748">DAPI</a> (blue; Tocris, Catalog # 5748).</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/descending-colon-organoids-743x240.jpg" alt="Human descending colon organoids stained with Chromogranin A and counterstained with E-Cadherin and DAPI." loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="organoidhandbook"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><strong>Video of Human Intestinal Organoid Grown in Cultrex UltiMatrix RGF BME.</strong> MUC2 (magenta) expression in human intestinal organoid grown using <a href="/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05">Cultrex UltiMatrix RGF Basement Membrane Extract</a> (R&D Systems, Catalog # BME001-05). Images courtesy of LifeCanvas Technologies.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="TZQ_W0ZvdOU" params="rel=0" style="background-image: url('https://img.youtube.com/vi/TZQ_W0ZvdOU/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/TZQ_W0ZvdOU?autoplay=1" class="lty-playbtn" title="Intestinal Organoid D28"> <span class="lyt-visually-hidden">Intestinal Organoid D28</span> </a> </lite-youtube> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--light"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/banners/cultrex-organoid-harvesting-solution-v3.jpg" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h1>Cultrex Organoid Harvesting Solution</h1></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h3>Ready to use • Non-enzymatic • Harvest intact organoids</h3><p><br> </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/cell-culture/cultrex-organoid-harvesting-solution_3700-100-01" class="btn btn-primary" type="link"> Learn More </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="literature"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Literature for Organoid Research</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/organoid-handbook" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/organoid-handbook-teasers-v4.jpg" alt=" Image of pages from the organoid handbook" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/organoid-handbook" > <h3 class="card_title_htag"> <div><h3>The Organoid Handbook</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>This handbook highlights some of the notable publications that have used organoids to investigate questions about development, disease, or drug safety. Additionally, it includes recipes and links to the key reagents that our own in-house scientists have used to establish the culture conditions for different types of organoids.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/organoid-handbook" > Download the Organoid Culture Handbook </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/scientific-articles/optimizing-organoid-culture-conditions" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/organoids-appnote-teaser-bg-v4.jpg" alt="Image of pages from the organoid culture application note" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/scientific-articles/optimizing-organoid-culture-conditions" > <h3 class="card_title_htag"> <div><h3>Optimizing Organoid Culture Conditions Application Note</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Establishing and optimizing the appropriate conditions for culturing different types of organoids can be technically challenging. Read this application note to learn about the importance of growth factor bioactivity and reagent consistency for achieving robust, reproducible organoid growth.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/scientific-articles/optimizing-organoid-culture-conditions" > Read the Organoid Culture Application Note </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/organoids-model-systems-of-tissue-development-and-disease-poster" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/organoid-wallposter-teasers-v4.jpg" alt="Image of the organoids wall poster" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/organoids-model-systems-of-tissue-development-and-disease-poster" > <h3 class="card_title_htag"> <div><h3>Organoids Wall Poster</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Organoids are emerging as powerful new platforms for exploring human organ development, modeling disease, screening for drug toxicity, and investigating personalized medicine. Request this poster to decorate your lab with some of the visually stunning organoid images that have been generated by cell and gene therapy scientists at Bio-Techne.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/organoids-model-systems-of-tissue-development-and-disease-poster" > Request the Organoids Wall Poster </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/pro-organoid-web-banner-1110x350.jpg" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Tissue Clearing Pro-Organoid</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Rapid, reversible and non-destructive clearing and staining kit.</p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/staining-kits-reagents/tissue-clearing-pro-organoid_7390" class="btn btn-primary" type="link"> Learn More </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="additional"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Additional Organoid Resources</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="cellculturemodelebook"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://info.proteinsimple.com/webinar-organoid-virtual-symposium-2020" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/human-descending-colon-organoid-525x315.jpg" alt="Image of human descending colon organoid" loading="lazy" > </div> </a> <a class="card_title_link" href="https://info.proteinsimple.com/webinar-organoid-virtual-symposium-2020" > <h3 class="card_title_htag"> <div><h3>Virtual Organoid Symposium</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Watch Bio-Techne’s Virtual Organoid Symposium from May 2020 to learn more about how leading scientists are utilizing organoids in their research. 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