Stem Cell Culture and Characterization Reagents and Assays

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href="/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05" > <h3 class="card_title_htag"> <div><h3>Cultrex™ UltiMatrix RGF Basement Membrane Extract</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul> <li>Designed for organoid and stem cell culture</li> <li>Protein composition and tensile strength optimized for 3D applications</li> <li>Qualified for dome formation</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/cell-culture/cultrex-ultimatrix-reduced-growth-factor-basement-membrane-extract_bme001-05" > Learn About UltiMatrix </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/promotions/excellerate-ipsc-medium-sample" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/tra-1-60-ipsc-525x315.jpg" alt="Fluorescence image of a human induced human induced pluripotent stem cell in ExCellerate iPSC Expansion Medium." loading="lazy" > </div> </a> <a class="card_title_link" href="/promotions/excellerate-ipsc-medium-sample" > <h3 class="card_title_htag"> <div><h3>New! ExCellerate™ GMP iPSC Expansion Medium</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul> <li>Completely animal-free</li> <li>All-in-one formulation</li> <li>Stable cell integrity over long term culture</li> </ul> <p> </p></div> </div> <div class="card-bottom-link"> <div> <a href="/promotions/excellerate-ipsc-medium-sample" > Try Our GMP iPSC Medium </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/p/proteins-enzymes/recombinant-human-bmp-4-gmp-protein-cf_314e-gmp" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/gmp-facility-bioreactor-525x315.jpg" alt="GMP manufacturing facility at Bio-Techne" loading="lazy" > </div> </a> <a class="card_title_link" href="/p/proteins-enzymes/recombinant-human-bmp-4-gmp-protein-cf_314e-gmp" > <h3 class="card_title_htag"> <div><h3>Animal-Free GMP BMP‑4</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><ul> <li>Equivalent bioactivity to our other BMP-4 proteins</li> <li>Animal-free for clinical manufacturing processes</li> <li>Backed by our quality standards and facility certifications. See <a href="/gmp-capabilities" target="_self">GMP Capabilities</a></li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/p/proteins-enzymes/recombinant-human-bmp-4-gmp-protein-cf_314e-gmp" > Learn About AF GMP BMP-4 </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h3>Why Use Bio-Techne Reagents for Culturing Stem Cells?</h3></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Our mission is to facilitate discovery and translation by ensuring high performance and lot-to-lot consistency of our products. Consistency is key to minimizing variability and standardizing protocols.</p> <p>Don’t take it from us, listen as Stem Cell Core Facility Directors describe why they prefer Bio-Techne proteins and small molecules for their cultures!</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <article> <div class="youtube-video-place"> <lite-youtube videoid="IUydCoe0yvQ" params="rel=0" style="background-image: url('https://img.youtube.com/vi/IUydCoe0yvQ/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/IUydCoe0yvQ?autoplay=1" class="lty-playbtn" title="Stem Cell Core Testimonials for Proteins and Small Molecules"> <span class="lyt-visually-hidden">Stem Cell Core Testimonials for Proteins and Small Molecules</span> </a> </lite-youtube> </div> </article> </div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_image_caption"> <div>Testimonial on the reliability of R&D Systems reagents for stem cell culture.</div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/serum-free-animal-free-teaser.jpg" alt="adherent cells in culgture supported by extracellular matrix" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/cell-culture-reagents/serum-free-animal-free" > <h3 class="card_title_htag"> <div>Serum-Free and Animal-Free Cell Culture</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Increase the consistency of your cell cultures as you approach translational studies for regenerative medicine and cell therapy programs. Adopting these media will</p> <ul> <li>Reduce variability in media composition</li> <li>Simplify compliance with regulatory guidelines</li> <li>Simplify comparability testing for raw material changes</li> </ul></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/cell-culture-reagents/serum-free-animal-free" > Culture Your Cells Serum-Free and Animal-Free </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/reagents/small-molecules-and-peptides/cept-cocktail" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cept_cocktail_teaser_card.png" alt="Stem Cell Cluster" loading="lazy" > </div> </a> <a class="card_title_link" href="/reagents/small-molecules-and-peptides/cept-cocktail" > <h3 class="card_title_htag"> <div>CEPT Cocktail for Improved Cell Survival</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Improve stem cell survival with our small molecule CEPT cocktail. A combination of 4 small molecules, <a href="/p/small-molecules-peptides/chroman-1_7163"><strong>C</strong>hroman 1</a>, <a href="/p/small-molecules-peptides/emricasan_7310"><strong>E</strong>mricasan</a>, <a href="/p/cell-culture/lyophilized-polyamine-supplement-x1000_7739">Lyophilized<strong> P</strong>olyamine Supplement</a> and <a href="/p/small-molecules-peptides/trans-isrib_5284"><strong>T</strong>rans-ISRIB</a>, the CEPT cocktail enhances the viability of stem cells during passaging, single cell cloning, gene-editing, cryopreservation, and differentiation and can be used in the manufacture of organoids for use in disease modeling or drug screening.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/reagents/small-molecules-and-peptides/cept-cocktail" > Learn About CEPT Cocktail </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <section class="paragraph paragraph--type--full-width-banner-with-image mb-4 paragraph--view-mode--default paragraph-background-color--dark"> <div class="banner_background_image" data-bgimage="https://resources.bio-techne.com/bio-techne-assets/images/banners/animal-free-proteins_background2.png" style="background-repeat: no-repeat;"> <div> <div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Animal-Free Recombinant Proteins</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Ensure a seamless transition from preclinical research into clinical manufacturing by starting your preclinical experiments with our Animal-Free RUO proteins. R&D Systems<sup>TM</sup> Animal-Free RUO proteins are produced in dedicated animal-free laboratories and are manufactured using the same systems as our Animal-Free GMP-Grade proteins.</p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/reagents/proteins/animal-free-proteins" class="btn btn-primary" type="link"> Browse Animal-Free Proteins </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/banners/animal-free-proteins_foreground.png" alt="Animal Free Proteins Banner" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </section> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3><a name="related"></a>Related Research Areas</h3> <figure class="responsive-figure-table"><table class="overflow-auto table table-bordered"> <tbody> <tr> <td class="w-50"><a href="/research-areas/cell-biology/hippo-signaling">HIPPO Signaling Pathway</a></td> <td><a href="/research-areas/epigenetics">Epigenetics</a></td> </tr> <tr> <td><a href="/research-areas/stem-cells/stem-cell-markers">Stem Cell Markers</a></td> <td><a href="/research-areas/stem-cells/stem-cell-signaling">Stem Cell Signaling</a></td> </tr> </tbody> </table></figure> <p> </p></div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="custom"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div><h3>Custom Services for Stem Cell Research</h3></div> <div><p>The multitude of potential stem cell preparations and applications means that the exact product you need for your research may not be available. If you can’t find what you need, our expert scientists and custom services team are here to help. We will work with you to deliver reagents and assays to meet your requirements. Whether it’s as simple as a change in formulation or a completely new product, take advantage of our resources and expertise to overcome your challenge.</p></div> <a href="/services/custom-cell-and-gene-therapy-services" class="btn btn-primary" type="link"> Inquire About Custom Services </a> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="regen"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--full-page-text-with-media mb-4 paragraph--view-mode--default"> <div><h3>Streamlining Translation for Regenerative Medicine</h3></div> <div><p>When it’s time to advance your <a href="/research-areas/regenerative-medicine">regenerative medicine</a> product to clinical manufacturing, partner with us for reliability, quality, and custom services. We will work with you to provide reproducible production of reagents and assays at clinical scale, with complete documentation. We offer <a href="/gmp-products/gmp-capabilities">GMP reagents</a> as well as 21 CFR Part 11-compliant<a href="/brands/proteinsimple"> analytical instruments</a> for automation and high throughput, including high sensitivity multiplex immunoassays for stem cell characterization with our <a href="/instruments/simple-western">Simple Western™</a>, <a href="/instruments/single-cell-western">Single-Cell Western</a>, and <a href="/instruments/simple-plex">Simple Plex™</a> product lines. We’re committed to helping you streamline the manufacture of regenerative medicine products.</p></div> <a href="/gmp-capabilities" class="btn btn-primary" type="link"> Learn About Our GMP Capabilities </a> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <div><h2>Stay Informed. New Solutions Are Coming.</h2></div> <a href="/research-areas/cell-and-gene-therapy-solutions/stay-informed" > <button type="button" class="btn btn-primary"> Subscribe </button> </a> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="resources"></a></p></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-resources-for-stem-cell-culture-and-differentiation22" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Resources for Stem Cell Culture and Differentiation</h5> </a> </div> <div class="collapse" id="expPara-resources-for-stem-cell-culture-and-differentiation22" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h3><strong>Website Stem Cell Resources</strong><br><br><strong>Webinars</strong></h3><ul><li><a href="/resources/webinars/view-all-webinars?tid_4%5B231%5D=231">View all Bio-Techne Stem Cell Webinars</a></li><li><a href="https://www.rndsystems.com/blog/advancing-culture-and-clonogenic-analysis-gastrointestinal-stem-cells" target="_blank">Advancing the Culture and Clonogenic Analysis of Gastrointestinal Stem Cells</a> at R&D Systems</li><li><a href="https://www.rndsystems.com/blog/isolation-and-culture-primary-cells-bone-marrow" target="_blank">Isolation and Culture of Primary Cells from Bone Marrow</a></li><li><a href="https://www.rndsystems.com/blog/webinar-regenerative-medicine-using-ipscs-setting-your-research-up-for-success" target="_blank">Regenerative Medicine using iPSCs</a></li></ul><h3><strong>Protocols</strong></h3><ul><li><a href="/resources/protocols-troubleshooting?tid_4%5B231%5D=231">View all Stem Cell Protocols</a></li><li><a href="https://www.rndsystems.com/protocol-types/stem-cell-protocols" target="_blank">Stem Cell Protocols</a> at R&D Systems</li><li><a href="https://www.tocris.com/resources/protocols/stem-cells" target="_blank">Protocols for Stem Cells</a> at Tocris</li></ul><h3><strong>Blogs</strong></h3><ul><li><a href="/resources/blogs">View all Stem Cell Blogs</a></li><li><a href="/resources/blogs/spheroids-vs-organoids-which-3d-cell-culture-model-is-best" rel="bookmark">Spheroids vs. Organoids: Which 3D Cell Culture Model is Best for You?</a></li></ul><h3><strong> Stem Cell eBooks</strong></h3><ul><li><a href="https://www.rndsystems.com/technical-guide-basement-membrane-basics" target="_blank">Technical Guide: Basement Membrane Basics</a> at R&D Systems</li><li><a href="https://www.rndsystems.com/resources/ebook/evolution-model-systems-toxicology-drug-screening-and-disease-modeling" target="_blank">Evolution of Cell Culture Models eBook</a> at R&D Systems</li><li><a href="https://info.proteinsimple.com/next-generation-analytical-solutions-cell-and-gene-therapy.html" target="_blank">Next-Generation Analytical Solutions For Cell & Gene Therapy eBook</a> at ProteinSimple</li><li><a href="//resources.bio-techne.com/bio-techne-assets/docs/literature/Stem_Cell_e-book_PL9-0008_RevA.pdf" target="_blank">Stem Cell Analysis: Advances in Western Blotting Technologies eBook</a></li></ul><h3><strong>Stem Cell Literature</strong></h3><ul><li><a href="https://resources.bio-techne.com/bio-techne-assets/images/literature/bio-techne-cultrex-matrices-stemcell-culture-flyer.pdf">Cultrex™ Matrices for Stem Cell Culture Flyer</a></li><li><a href="/resources/literature/stem-cell-differentiation-on-simple-western">Unraveling the Signaling Web in Stem Cells for Regenerative Medicine with Simple Western</a></li><li><a href="//resources.bio-techne.com/bio-techne-assets/images/pdfs/an-ipsc-morphology-differentiation.pdf" target="_blank">Application Note: Assessing iPSC Colony Morphology Attributes</a></li><li><a href="https://resources.rndsystems.com/images/site/br_ESiPS_MKT20-10863.pdf" target="_blank">Embryonic and Induced Pluripotent Stem Cell Workflow Solutions Brochure</a> at R&D Systems</li><li><a href="https://resources.tocris.com/pdfs/literature/posters/stem-cell-workflow-poster-v1-2019.pdf" target="_blank">Stem Cell Workflow: Using Small Molecules Poster</a> at Tocris</li><li><a href="https://resources.tocris.com/pdfs/literature/posters/stemcells-poster-2018.pdf" target="_blank">Stem Cells and Differentiation Poster</a> at Tocris</li><li><a href="/resources/literature/brochure-stem-cell-guide" target="_blank">Small Molecules for Stem Cell Research Product Guide</a></li><li><a href="https://www.bio-techne.com/resources/literature/proteins-for-stem-cell-culture-guide">Proteins for Stem Cell Culture Guide</a></li><li><a href="https://www.rndsystems.com/resources/articles/technical-notes-assessing-pluripotent-status-stem-cells" target="_blank">Technical Notes: Assessing the Pluripotent Status of Stem Cells</a> at R&D Systems</li><li><a href="https://www.rndsystems.com/resources/articles/differentiation-potential-induced-pluripotent-stem-cells" target="_blank">Differentiation potential of iPSC</a> at R&D Systems</li><li><a href="/resources/literature/mending-myocardium-tracking-cardiomyocyte-differentiation-single-cell-westerns">Mending the Myocardium: Tracking Cardiomyocyte Differentiation with Single-Cell Westerns</a></li><li><a href="https://www.rndsystems.com/resources/posters/hematopoietic-colony-identification-guide-wall-poster" target="_blank">Hematopoietic Colony Identification Guide Wall Poster</a></li></ul><h3>Stem Cell-related Tools</h3><ul><li><a href="https://www.rndsystems.com/resources/cell-markers/stem-cells" target="_blank">Stem Cells Markers Interactive Tool</a> at R&D Systems</li><li><a href="/resources/spectra-viewer">Spectra Viewer</a> </li><li><a href="/resources/flow-cytometry-panel-builder" target="_blank">Flow Cytometry Panel Builder</a> </li></ul><h3>Stem Cell Pathways</h3><ul><li><a href="/resources/signal-transduction-pathways/hedgehog-pathway">Hedgehog Signaling Pathway</a></li><li><a href="/resources/signal-transduction-pathways/tgf-beta-pathway">TGF-β Signaling Pathway</a></li><li><a href="https://www.rndsystems.com/pathways/embryonic-induced-pluripotent-stem-cell-differentiation-pathways-lineage-specific-markers">Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers</a> (at R&D Systems)</li><li><a href="https://www.rndsystems.com/pathways/mesenchymal-stem-cell-differentiation-pathways-lineage-specific-markers">Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers</a> (at R&D Systems)</li><li><a href="https://www.rndsystems.com/pathways/neural-stem-cell-differentiation-pathways-lineage-specific-markers">Neural Stem Cell Differentiation Pathways & Lineage-specific Markers</a> (at R&D Systems)</li><li><a href="https://www.rndsystems.com/pathways/hematopoietic-stem-cell-differentiation-pathways-lineage-specific-markers">Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers</a> (at R&D Systems)</li></ul></div> </div> </div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-video-transcript-for-why-use-bio-techne-reagents-for-culturing-stem-cells24" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Video Transcript for “Why Use Bio-Techne Reagents for Culturing Stem Cells?"</h5> </a> </div> <div class="collapse" id="expPara-video-transcript-for-why-use-bio-techne-reagents-for-culturing-stem-cells24" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><p>I have been using R&D / Bio-Techne perhaps for the last maybe 20 years, ever since I was a graduate student. My lab would exclusively use R&D products. I've found R&D / Bio-Techne to be extremely reliable.</p> <p>The Bio-Techne products that we’re using, we love and what we're using are cytokines, growth factors and we have been using them since the beginning. I mean our core started in 2008 and we really have not changed.</p> <p>So in our stem cell core facility, we do a lot of differentiation into different progenies. For all this differentiation we use a number of different cytokines, growth factors and we buy them all from R&D Systems or Tocris.</p> <p>We had very good experience with R&D cytokines as well as antibodies. We didn't find any lot-to-lot variation with the cytokines and antibodies. So, in general, we're very pleased.</p> <p>The product, the reproducibility has been consistent, and we've seen that everything works, everything is good, and now a lot of the products we buy and we don't lot test and we haven't had issues. Everything has been excellent and has worked beautifully.</p></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h5><a name="about"></a></h5> <h3>About Stem Cells</h3> <p>The defining characteristics of stem cells, <em>i.e.</em> their ability to self-renew and differentiate, make them a valuable resource in medical research and therapy. Directed differentiation of stem cells can provide insights into embryonic development, generate organ-like structures (<strong><a href="/research-areas/organoids-3d-culture" target="_self">organoids</a></strong>), and help us understand complex disease processes.</p> <p>Stem cells are unspecialized cells that are capable of self-renewal through mitotic cell division, even after long periods of inactivity. Stem cell differentiation gives rise to the endoderm, ectoderm, and mesoderm germ layers which develop into the specialized cells of a tissues and organs.</p> <p>Embryonic stem cells (ESCs) are found in the inner cell mass of blastocysts, adult (or somatic) stem cells maintain the populations of specific cell types, <strong><a href="/research-areas/stem-cells/ipsc" target="_self">induced pluripotent stem cells</a></strong> (iPSCs) are produced by the reprogramming of somatic cells, and cancer stem cells (CSCs) reside in tissues where they give rise to local tumors. Hematopoietic stem cells (HSC) differentiate into the myeloid and lymphoid immune cell lineages. Multipotent <a href="/research-areas/stem-cells/mesenchymal-stem-cells">mesenchymal stem cells</a> (MSC) give rise to adipocytes, chondrocytes, osteocytes, and cardiomyocytes. <a href="/research-areas/stem-cells/neural-stem-cells">Neural stem cells</a> (NSC) can differentiate into neurons, astrocytes, and oligodendrocytes.</p> <p>Owing to their tissue regenerative potential, stem cells can be utilized to repair or replace old or damaged cells, and this property is valuable in <strong><a href="/research-areas/regenerative-medicine">regenerative medicine</a></strong>. Stem cells offer the potential for treating many diseases including <a href="/research-areas/cancer">cancer</a>, <a href="/research-areas/neuroscience/neurodegeneration">neurodegeneration</a>, musculoskeletal disorders, heart disease, and diabetes. 3-dimensional cultures and organoids based on differentiated stem cells reproduce some functions of native tissues and are valuable for toxicology studies and drug discovery.</p></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-different-types-of-stem-cells-are-identifiable-by-their-specification-potential10" role="button" aria-expanded="false" aria-controls="expPara"> <h5>Different types of stem cells are identifiable by their specification potential</h5> </a> </div> <div class="collapse" id="expPara-different-types-of-stem-cells-are-identifiable-by-their-specification-potential10" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/somatic-cells-figure.png" alt="Stem cells have diverse differentiation potential and may be classified as totipotent, pluripotent, multipotent or unipotent." loading="lazy" > </div> <br/> <div><p><strong>Totipotent stem cells:</strong> generate all the cell types in the organism (e.g., zygote or fertilized egg).</p> <p><strong>Pluripotent stem cells:</strong> generate all the embryonic germ layers (i.e. endoderm, ectoderm and mesoderm), but are unable to generate extra-embryonic tissues (i.e. placenta). Best exemplified by embryonic stem cells (ESCs) which are derived from the inner cell mass of the blastocyst. Induced pluripotent stem cells (iPSCs) are reprogrammed from adult differentiated cells (e.g., fibroblasts, peripheral blood mononuclear cells).</p> <p><strong>Multipotent stem cells:</strong> generate a limited number of cell types based on their tissue of origin.</p> <p>Mesenchymal stem cells- give rise to fat, bone, muscle and cartilage.<br> Hematopoietic stem cells- give rise to different types of blood cells (i.e. platelets, red and white cells).<br> Neural stem cells- give rise to neurons, oligodendrocytes and astrocytes.</p> <p><strong>Oligopotent stem cells:</strong> generate few cell types which are closely related (e.g., myeloid stem cells).</p> <p><strong>Unipotent stem cells:</strong> generate one cell type (e.g., epidermal stem cells, muscle stem cells).</p></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-3-media-to-text paragraph--view-mode--default mb-4 p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--25-75"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/dw-ges-page-img-4-v2.png" alt="TcBuster graphic" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:1_3_media_to_text:field_header_2_global"> <div><h4>Non-Viral Gene Transfer With TcBuster</h4></div> </div> <div class="field_block:paragraph:1_3_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p> </p><p>Realize the potential of gene engineering by partnering with our experts. 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