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href="https://independent.academia.edu/DanielleLarouche">Danielle Larouche</a></div><p class="ds-related-work--metadata ds2-5-body-xs">BioResearch Open Access, 2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries","attachmentId":111684448,"attachmentType":"pdf","work_url":"https://www.academia.edu/115210333/Improved_Methods_to_Produce_Tissue_Engineered_Skin_Substitutes_Suitable_for_the_Permanent_Closure_of_Full_Thickness_Skin_Injuries","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/115210333/Improved_Methods_to_Produce_Tissue_Engineered_Skin_Substitutes_Suitable_for_the_Permanent_Closure_of_Full_Thickness_Skin_Injuries"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="107365496" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/107365496/Tissue_engineered_skin_substitutes_from_in_vitro_constructs_to_in_vivo_applications">Tissue-engineered skin substitutes: from in vitro constructs to in vivo applications</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37099636" href="https://independent.academia.edu/RoxanePouliot">Roxane Pouliot</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Biotechnology and applied biochemistry, 2004</p><p class="ds-related-work--abstract ds2-5-body-sm">The field of skin tissue engineering is a paradigm for the various efforts towards the reconstruction of other tissues and organ substitutes. As skin replacement, this biotechnological approach has evolved from simple cultured autologous epidermal sheets to more complex bilayered cutaneous substitutes. The various types of such substitutes are herein presented with their intended use. However, two integrative characteristics are analysed more specifically because of their critical role: neovascularization and re-innervation. Furthermore, the in vitro use of these various skin substitutes has shed light on various physiological and pathological phenomena. Thus, not only the in vivo application of these skin substitutes as grafts, but also their in vitro value as skin models, are presented.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Tissue-engineered skin substitutes: from in vitro constructs to in vivo applications","attachmentId":106051085,"attachmentType":"pdf","work_url":"https://www.academia.edu/107365496/Tissue_engineered_skin_substitutes_from_in_vitro_constructs_to_in_vivo_applications","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/107365496/Tissue_engineered_skin_substitutes_from_in_vitro_constructs_to_in_vivo_applications"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="63365048" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/63365048/Engineered_skin_substitutes_practices_and_potentials">Engineered skin substitutes: practices and potentials</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45981986" href="https://independent.academia.edu/DorothySupp">Dorothy Supp</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinics in Dermatology, 2005</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Engineered skin substitutes: practices and potentials","attachmentId":75818395,"attachmentType":"pdf","work_url":"https://www.academia.edu/63365048/Engineered_skin_substitutes_practices_and_potentials","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/63365048/Engineered_skin_substitutes_practices_and_potentials"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="17809204" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/17809204/Tissue_engineering_of_cultured_skin_substitutes">Tissue engineering of cultured skin substitutes</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37732417" href="https://independent.academia.edu/RHorch">Raymund Horch</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Cellular and Molecular Medicine, 2005</p><p class="ds-related-work--abstract ds2-5-body-sm">Skin replacement has been a challenging task for surgeons ever since the introduction of skin grafts by Reverdin in 1871. Recently, skin grafting has evolved from the initial autograft and allograft preparations to biosynthetic and tissue-engineered living skin replacements. This has been fostered by the dramatically improved survival rates of major burns where the availability of autologous normal skin for grafting has become one of the limiting factors. The ideal properties of a temporary and a permanent skin substitute have been well defined. Tissue-engineered skin replacements: cultured autologous keratinocyte grafts, cultured allogeneic keratinocyte grafts, autologous/allogeneic composites, acellular biological matrices, and cellular matrices including such biological substances as fibrin sealant and various types of collagen, hyaluronic acid etc. have opened new horizons to deal with such massive skin loss. In extensive burns it has been shown that skin substitution with cultured grafts can be a life-saving measure where few alternatives exist. Future research will aim to create skin substitutes with cultured epidermis that under appropriate circumstances may provide a wound cover that could be just as durable and esthetically acceptable as conventional split-thickness skin grafts. Genetic manipulation may in addition enhance the performance of such cultured skin substitutes. If cell science, molecular biology, genetic engineering, material science and clinical expertise join their efforts to develop optimized cell culture techniques and synthetic or biological matrices then further technical advances might well lead to the production of almost skin like new tissue-engineered human skin products resembling natural human skin.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Tissue engineering of cultured skin substitutes","attachmentId":42227843,"attachmentType":"pdf","work_url":"https://www.academia.edu/17809204/Tissue_engineering_of_cultured_skin_substitutes","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17809204/Tissue_engineering_of_cultured_skin_substitutes"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="25583452" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/25583452/Tissue_engineered_skin_substitutes_an_overview">Tissue-engineered skin substitutes: an overview</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="49118691" href="https://uniupo.academia.edu/BarbaraAzzimonti">Barbara Azzimonti</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Artificial Organs, 2013</p><div class="ds-related-work--ctas"><button 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