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Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that" /> <meta name="twitter:image" content="https://0.academia-photos.com/201065409/62462587/50754242/s200_krupa.sudheendra.png" /> <meta property="fb:app_id" content="2369844204" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization" /> <meta property="og:title" content="3D Bioprinting for Vascularization" /> <meta property="og:image" content="http://a.academia-assets.com/images/open-graph-icons/fb-paper.gif" /> <meta property="og:description" content="In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that" /> <meta property="article:author" content="https://independent.academia.edu/KrupaSudheendra" /> <meta name="description" content="In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that" /> <title>(PDF) 3D Bioprinting for Vascularization | Krupa Sudheendra - Academia.edu</title> <link rel="canonical" href="https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '92477ec68c09d28ae4730a4143c926f074776319'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1732855486000); window.Aedu.timeDifference = new Date().getTime() - 1732855486000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that must be observed in order to develop successful 3D tissue constructs for clinical applications. However, current research in 3D bioprinting model development lacks diverse methods of successful vascularization as a result of issues with scaling, size, and variations in printing method. This study analyzes the methods of printing, bioinks used, and analysis techniques in 3D bioprinting for vascularization. These methods are discussed and evaluated to determine the most optimal strategies of 3D bioprinting for successful vascularization. Integrating stem and endothelial cells in prints, selecting the type of bioink according to its physical properties, and choosing a printing method according to physical properties of the desired printed ...","author":[{"@context":"https://schema.org","@type":"Person","name":"Krupa Sudheendra"}],"contributor":[],"dateCreated":"2023-07-11","dateModified":null,"datePublished":null,"headline":"3D Bioprinting for Vascularization","inLanguage":"en","keywords":["Bioengineering"],"locationCreated":null,"publication":"Bioengineering","publisher":{"@context":"https://schema.org","@type":"Organization","name":"MDPI AG"},"image":null,"thumbnailUrl":null,"url":"https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization","sourceOrganization":[{"@context":"https://schema.org","@type":"EducationalOrganization","name":null}]}</script><link rel="stylesheet" media="all" 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Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that must be observed in order to develop successful 3D tissue constructs for clinical applications. However, current research in 3D bioprinting model development lacks diverse methods of successful vascularization as a result of issues with scaling, size, and variations in printing method. This study analyzes the methods of printing, bioinks used, and analysis techniques in 3D bioprinting for vascularization. These methods are discussed and evaluated to determine the most optimal strategies of 3D bioprinting for successful vascularization. Integrating stem and endothelial cells in prints, selecting the type of bioink according to its physical properties, and choosing a printing method according to physical properties of the desired printed ...","publisher":"MDPI AG","publication_name":"Bioengineering"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"3D Bioprinting for Vascularization","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [201065409]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":104194091,"attachmentType":"pdf"}"><img alt="First page of “3D Bioprinting for Vascularization”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/104194091/mini_magick20230711-1-ii3oed.png?1689100705" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">3D Bioprinting for Vascularization</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="201065409" href="https://independent.academia.edu/KrupaSudheendra"><img alt="Profile image of Krupa Sudheendra" class="ds-work-card--author-avatar" src="https://0.academia-photos.com/201065409/62462587/50754242/s65_krupa.sudheendra.png" />Krupa Sudheendra</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">Bioengineering</p></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">In the world of clinic treatments, 3D-printed tissue constructs have emerged as a less invasive treatment method for various ailments. Printing processes, scaffold and scaffold free materials, cells used, and imaging for analysis are all factors that must be observed in order to develop successful 3D tissue constructs for clinical applications. However, current research in 3D bioprinting model development lacks diverse methods of successful vascularization as a result of issues with scaling, size, and variations in printing method. This study analyzes the methods of printing, bioinks used, and analysis techniques in 3D bioprinting for vascularization. These methods are discussed and evaluated to determine the most optimal strategies of 3D bioprinting for successful vascularization. Integrating stem and endothelial cells in prints, selecting the type of bioink according to its physical properties, and choosing a printing method according to physical properties of the desired printed ...</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":104194091,"attachmentType":"pdf","workUrl":"https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":104194091,"attachmentType":"pdf","workUrl":"https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="104194091" data-landing_url="https://www.academia.edu/104474089/3D_Bioprinting_for_Vascularization" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="104729583" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/104729583/3D_Bioprinting_for_Vascularized_Tissue_Fabrication">3D Bioprinting for Vascularized Tissue Fabrication</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="38841160" href="https://musc.academia.edu/MichaelYost">Michael Yost</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Annals of biomedical engineering, 2016</p><p class="ds-related-work--abstract ds2-5-body-sm">3D bioprinting holds remarkable promise for rapid fabrication of 3D tissue engineering constructs. Given its scalability, reproducibility, and precise multi-dimensional control that traditional fabrication methods do not provide, 3D bioprinting provides a powerful means to address one of the major challenges in tissue engineering: vascularization. Moderate success of current tissue engineering strategies have been attributed to the current inability to fabricate thick tissue engineering constructs that contain endogenous, engineered vasculature or nutrient channels that can integrate with the host tissue. Successful fabrication of a vascularized tissue construct requires synergy between high throughput, high-resolution bioprinting of larger perfusable channels and instructive bioink that promotes angiogenic sprouting and neovascularization. This review aims to cover the recent progress in the field of 3D bioprinting of vascularized tissues. It will cover the methods of bioprinting v...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"3D Bioprinting for Vascularized Tissue Fabrication","attachmentId":104380918,"attachmentType":"pdf","work_url":"https://www.academia.edu/104729583/3D_Bioprinting_for_Vascularized_Tissue_Fabrication","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/104729583/3D_Bioprinting_for_Vascularized_Tissue_Fabrication"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="51766218" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/51766218/Mini_review_advances_in_3D_bioprinting_of_vascularized_constructs">Mini-review: advances in 3D bioprinting of vascularized constructs</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="41772878" href="https://ucla.academia.edu/FBilli">Fabrizio Billi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Biology Direct</p><p class="ds-related-work--abstract ds2-5-body-sm">3D in vitro constructs have gained more and more relevance in tissue engineering and in cancer-modeling. In recent years, with the development of thicker and more physiologically relevant tissue patches, the integration of a vascular network has become pivotal, both for sustaining the construct in vitro and to help the integration with the host tissue once implanted. Since 3D bioprinting is rising to be one of the most versatile methods to create vascularized constructs, we here briefly review the most promising advances in bioprinting techniques.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Mini-review: advances in 3D bioprinting of vascularized constructs","attachmentId":69343990,"attachmentType":"pdf","work_url":"https://www.academia.edu/51766218/Mini_review_advances_in_3D_bioprinting_of_vascularized_constructs","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/51766218/Mini_review_advances_in_3D_bioprinting_of_vascularized_constructs"><span 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data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Use of 3D-BIOPRINTING in Tissue Engineering Scaffold Production","attachmentId":114179680,"attachmentType":"pdf","work_url":"https://www.academia.edu/118589680/Use_of_3D_BIOPRINTING_in_Tissue_Engineering_Scaffold_Production","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/118589680/Use_of_3D_BIOPRINTING_in_Tissue_Engineering_Scaffold_Production"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="58266749" data-sort-order="default"><a 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translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/58266749/Bioprinting_in_Vascularization_Strategies"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="77320982" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/77320982/3D_Bioprinting_for_Tissue_Engineering_Application_Review">3D Bioprinting for Tissue Engineering Application Review</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="207234115" href="https://independent.academia.edu/MagedNaser1">Maged Naser</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2021</p><p class="ds-related-work--abstract ds2-5-body-sm">Three-dimensional (3D) printing (rapid prototyping or additive fabricating innovations) has gotten significant consideration in different fields in the course of recent many years. Tissue engineering uses of 3D bioprinting, specifically, have attracted the attention of numerous researchers. 3D platforms delivered by the 3D bioprinting of biomaterials (bio-inks) empower the recovery and rebuilding of different tissues and organs. These 3D bioprinting methods are helpful for creating platforms for biomedical and regenerative medication and tissue engineering applications, allowing quick production with high-accuracy and control over size, porosity, and shape. In this review, we present an assortment of tissue designing applications to make bones, vascular, skin, ligament, and neural structures utilizing an assortment of 3D bioprinting strategies.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"3D Bioprinting for Tissue Engineering Application Review","attachmentId":84729230,"attachmentType":"pdf","work_url":"https://www.academia.edu/77320982/3D_Bioprinting_for_Tissue_Engineering_Application_Review","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/77320982/3D_Bioprinting_for_Tissue_Engineering_Application_Review"><span 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data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Trends of 3D bioprinting in vascular surgery","attachmentId":96770252,"attachmentType":"pdf","work_url":"https://www.academia.edu/94264897/Trends_of_3D_bioprinting_in_vascular_surgery","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/94264897/Trends_of_3D_bioprinting_in_vascular_surgery"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="109593084" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/109593084/A_bioink_blend_for_rotary_3D_bioprinting_tissue_engineered_small_diameter_vascular_constructs">A bioink blend for rotary 3D bioprinting tissue engineered small-diameter vascular constructs</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="13432816" href="https://independent.academia.edu/PranavSoman">Pranav Soman</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Acta Biomaterialia, 2019</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"A bioink blend for rotary 3D bioprinting tissue engineered small-diameter vascular constructs","attachmentId":107671369,"attachmentType":"pdf","work_url":"https://www.academia.edu/109593084/A_bioink_blend_for_rotary_3D_bioprinting_tissue_engineered_small_diameter_vascular_constructs","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/109593084/A_bioink_blend_for_rotary_3D_bioprinting_tissue_engineered_small_diameter_vascular_constructs"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="118589643" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md 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Such tissue engineering field is&lt;br&gt; nowadays recognized to possess high potential in human curing and fast treating of patients in&lt;br&gt; personalized medicine sense. 3D bioprinting approach is interconnected with many scientific fields,&lt;br&gt; such as engineering, mathematics, biology, and medicine.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Optimization of Parameters for 3D-Bioprinting Scaffold Production – Blood Vessel Bioengineering","attachmentId":114179666,"attachmentType":"pdf","work_url":"https://www.academia.edu/118589643/Optimization_of_Parameters_for_3D_Bioprinting_Scaffold_Production_Blood_Vessel_Bioengineering","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a 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