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data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598982/Cationic_ceramides_and_analogues_LCL30_and_LCL85_as_adjuvants_to_photodynamic_therapy_of_tumors"><img alt="Research paper thumbnail of Cationic ceramides and analogues, LCL30 and LCL85, as adjuvants to photodynamic therapy of tumors" class="work-thumbnail" src="https://attachments.academia-assets.com/106223493/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598982/Cationic_ceramides_and_analogues_LCL30_and_LCL85_as_adjuvants_to_photodynamic_therapy_of_tumors">Cationic ceramides and analogues, LCL30 and LCL85, as adjuvants to photodynamic therapy of tumors</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Sep 1, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Photodynamic therapy (PDT) is known to alter the expression of various genes in treated cells. Th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Photodynamic therapy (PDT) is known to alter the expression of various genes in treated cells. This prompted us to examine the activity of genes encoding two important enzymes in sphingolipid (SL) metabolism, dihydroceramide desaturase (DES) and sphingosine kinase (SPHK), in mouse SCCVII tumor cells treated by PDT using either the porphyrin-based photosensitizer Photofrin or silicon phthalocyanine Pc4. The results revealed that PDT induced an upregulation in the expression of two major isoforms of both genes (DES1 and DES2 as well as SPHK1 and SPHK2). While the changes were generally moderate (2-3 fold gains), the increase in DES2 expression was more pronounced and it was much greater with Photofrin-PDT than with Pc4-PDT (over 23-fold vs. less than 5-fold). Combining either Photofrin-PDT or Pc4-PDT with the cationic C16-ceramide LCL30 (20 mg/kg i.p.) for treatment of subcutaneously growing SCCVII tumors rendered important differences in the therapy outcome. Photofrin-PDT, used at a dose that attained good initial response but no tumor cures, produced 50% cures when combined with a single LCL30 treatment. In contrast, the same LCL30 treatment combined with Pc4-PDT had no significant effect on tumor response. The optimal timing of LCL30 injection was immediately after Photofrin-PDT. The therapeutic benefit was lost when LCL30 was given in two 20 mg/kg injections encompassing intervals before and after PDT. LCL85, the cationic B13 ceramide analogue and SL-modulating agent, also increased cure rates of Photofrin-PDT treated tumors, but the therapeutic benefit was less pronounced than with LCL30. These results with LCL30 and LCL85, and our previous findings for LCL29 (another SL analogue), assert the potential of SLs for use as adjuvants to augment the efficacy of PDT-mediated tumor destruction.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="28685846537bb02a0ceadab21b0df964" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223493,"asset_id":107598982,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223493/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598982"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598982"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598982; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598981"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598981/Antitumor_Efficacy_of_Photodynamic_Therapy_Using_Novel_Nanoformulations_of_Hypocrellin_Photosensitizer_SL052"><img alt="Research paper thumbnail of Antitumor Efficacy of Photodynamic Therapy Using Novel Nanoformulations of Hypocrellin Photosensitizer SL052" class="work-thumbnail" src="https://attachments.academia-assets.com/106223510/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598981/Antitumor_Efficacy_of_Photodynamic_Therapy_Using_Novel_Nanoformulations_of_Hypocrellin_Photosensitizer_SL052">Antitumor Efficacy of Photodynamic Therapy Using Novel Nanoformulations of Hypocrellin Photosensitizer SL052</a></div><div class="wp-workCard_item"><span>Photochemistry and Photobiology</span><span>, Dec 20, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recent preclinical and clinical testing of hypocrellin-based photosensitizer SL052 for use in pho...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recent preclinical and clinical testing of hypocrellin-based photosensitizer SL052 for use in photodynamic therapy (PDT) of cancer has shown encouraging results. Further optimization of its formulation for delivery could considerably extend the therapeutic efficiency of this drug. A nanoformulation encapsulating SL052 into biodegradable polymer poly(lactic-co-glycolic acid) (PLGA) was developed using a single-emulsion solvent evaporation technique and characterized in terms of particle size and loading of the photosensitizing agent. This nanoformulation, SL052-PLGA-nanoparticles (NPs), was compared with recently created nanoformulation based on polyvinylpyrrolidone (SL052-PVP-NPs) and standard liposomal SL052 preparation in terms of efficacy when used for PDT treatment of squamous cell carcinomas SCCVII growing subcutaneously in syngeneic mice. The therapeutic effect of PDT using these three different SL052 formulations was tested for both 1 and 4 h intervals between drug injection and tumor light exposure. The longer time interval produced higher tumor cure rates with all SL052 preparations. With both drug-light intervals, PDT based on SL052-PLGA-NPs produced superior therapeutic benefit compared with the other two SL052 formulations.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f879f2e94b041ce07544a38f6b2993f5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223510,"asset_id":107598981,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223510/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598981"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598981"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598981; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598980"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598980/Increased_killing_of_SCCVII_squamous_cell_carcinoma_cells_after_the_combination_of_Pc_4_photodynamic_therapy_and_dasatinib_is_associated_with_enhanced_caspase_3_activity_and_ceramide_synthase_1_upregulation"><img alt="Research paper thumbnail of Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598980/Increased_killing_of_SCCVII_squamous_cell_carcinoma_cells_after_the_combination_of_Pc_4_photodynamic_therapy_and_dasatinib_is_associated_with_enhanced_caspase_3_activity_and_ceramide_synthase_1_upregulation">Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation</a></div><div class="wp-workCard_item"><span>International Journal of Oncology</span><span>, Oct 9, 2013</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598980"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598980"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598980; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598980]").text(description); $(".js-view-count[data-work-id=107598980]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598980; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598980']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598980]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598980,"title":"Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation","internal_url":"https://www.academia.edu/107598980/Increased_killing_of_SCCVII_squamous_cell_carcinoma_cells_after_the_combination_of_Pc_4_photodynamic_therapy_and_dasatinib_is_associated_with_enhanced_caspase_3_activity_and_ceramide_synthase_1_upregulation","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598979"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598979/Enhancement_of_laser_cancer_treatment_by_a_chitosan_derived_immunoadjuvant"><img alt="Research paper thumbnail of Enhancement of laser cancer treatment by a chitosan-derived immunoadjuvant" class="work-thumbnail" src="https://attachments.academia-assets.com/106223522/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598979/Enhancement_of_laser_cancer_treatment_by_a_chitosan_derived_immunoadjuvant">Enhancement of laser cancer treatment by a chitosan-derived immunoadjuvant</a></div><div class="wp-workCard_item"><span>Photochemistry and Photobiology</span><span>, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A chitosan derivative, glycated chitosan (GC), has been used as an immunostimulant for cancer tre...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A chitosan derivative, glycated chitosan (GC), has been used as an immunostimulant for cancer treatment in laser immunotherapy. The function of GC is to enhance the host immune response after direct cancer cell destruction by a selective laser photothermal interaction. To further test its effects, laser immunotherapy was extended to include several different adjuvants for immunological stimulation and to include photodynamic therapy (PDT) as a different tumordestruction mechanism. Complete Freund (CF) adjuvant, incomplete Freund (IF) adjuvant and Corynebucterium parvum (CP) were selected for treatment of metastatic mammary tumors in rats, in combination with a selective photothermal interaction. The solution of the immunoadjuvants admixed with indocyanine green (ICG), a light-absorbing dye, was injected directly into the tumors, followed by noninvasive irradiation of an 805 nm laser. Combined with PDT, in the treatment of tumors in mice, GC was administered peritumorally immediately after laser irradiation. The survivals of treated animals were compared with untreated control animals. In the treatment of rat tumors, CF, IF and CP raised the cure rates from 0% to 18%, 7% and 9%, respectively. In comparison, GC resulted in a 29% long-term survival. In the treatment of EMT6 mammary sarcoma in mice, GC of 0.5% and 1.5% concentrations increased the cure rates of Photofrin-based PDT treatment from 38% to 63% and 75%, respectively. In the treatment of Line 1 lung adenocarcinoma in mice, a 1.67% GC solution enabled a noncurative mesosubstituted tetra(meta-hydroxy-pheny1)chlorin-based PDT to YPosted on the website on 9 November 2004.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="266b1a3f0f119b9ebc36de1f94fce810" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223522,"asset_id":107598979,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223522/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598979"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598979"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598979; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598979]").text(description); $(".js-view-count[data-work-id=107598979]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598979; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598979']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "266b1a3f0f119b9ebc36de1f94fce810" } } $('.js-work-strip[data-work-id=107598979]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598979,"title":"Enhancement of laser cancer treatment by a chitosan-derived immunoadjuvant","internal_url":"https://www.academia.edu/107598979/Enhancement_of_laser_cancer_treatment_by_a_chitosan_derived_immunoadjuvant","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[{"id":106223522,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223522/thumbnails/1.jpg","file_name":"j.1751-1097.2005.tb01541.x20231004-1-9surwd.pdf","download_url":"https://www.academia.edu/attachments/106223522/download_file","bulk_download_file_name":"Enhancement_of_laser_cancer_treatment_by.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223522/j.1751-1097.2005.tb01541.x20231004-1-9surwd-libre.pdf?1696453825=\u0026response-content-disposition=attachment%3B+filename%3DEnhancement_of_laser_cancer_treatment_by.pdf\u0026Expires=1740033853\u0026Signature=CAnVHKacvv~TWY8Cv7mOtftvCebEnjSXa-mLnqITRiRas3e0VpqM5N1n~n7QLmfF~oUjgQnmxrn01UVXJpUDUOQfkrJMN30kDy59BaZ3OSAnVSh2igrqo9O7rqABu-SEUV-6BKb8o3VNqFlJzmJI3FNAHDrWw0195QXuvWcFki6KDJ4bSJNfucOzIKQL5XMdDAo6mFgQFdnX2Zdo9lfwqSRKt-5pcxzyXIN0cds7gdHEoQYNYBYCb1u3-gaQ86Em4GFPUXgoqrDcw7XEtdSgNTlSeeer46H6DtuYbY7dr6l-9sTRemFKzn8dYno24qfzzjUgNpW8~n2eGL-PZdUAIA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598978"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598978/_title_Laser_immunotherapy_a_novel_approach_for_metastatic_tumors_title_"><img alt="Research paper thumbnail of <title>Laser immunotherapy: a novel approach for metastatic tumors</title>" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598978/_title_Laser_immunotherapy_a_novel_approach_for_metastatic_tumors_title_"><title>Laser immunotherapy: a novel approach for metastatic tumors</title></a></div><div class="wp-workCard_item"><span>SPIE Proceedings</span><span>, Aug 20, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598978"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598978"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598978; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598978]").text(description); $(".js-view-count[data-work-id=107598978]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598978; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598978']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598978]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598978,"title":"\u003ctitle\u003eLaser immunotherapy: a novel approach for metastatic tumors\u003c/title\u003e","internal_url":"https://www.academia.edu/107598978/_title_Laser_immunotherapy_a_novel_approach_for_metastatic_tumors_title_","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598977"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598977/Ceramide_synthase_inhibitor_fumonisin_B1_inhibits_apoptotic_cell_death_in_SCC17B_human_head_and_neck_squamous_carcinoma_cells_after_Pc4_photosensitization"><img alt="Research paper thumbnail of Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization" class="work-thumbnail" src="https://attachments.academia-assets.com/106223491/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598977/Ceramide_synthase_inhibitor_fumonisin_B1_inhibits_apoptotic_cell_death_in_SCC17B_human_head_and_neck_squamous_carcinoma_cells_after_Pc4_photosensitization">Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, Nov 1, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The sphingolipid ceramide modulates stress-induced cell death and apoptosis. We have shown that c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The sphingolipid ceramide modulates stress-induced cell death and apoptosis. We have shown that ceramide generated via de novo sphingolipid biosynthesis is required to initiate apoptosis after photodynamic therapy (PDT). The objective of this study was to define the role of ceramide synthase (CERS) in PDT-induced cell death and apoptosis using fumonisin B1 (FB), a CERS inhibitor. We used the silicon phthalocyanine Pc4 for PDT, and SCC17B cells, as a clinically-relevant model of human head and neck squamous carcinoma. zVAD-fmk, a pan-caspase inhibitor, as well as FB, protected cells from death after PDT. In contrast, ABT199, an inhibitor of the anti-apoptotic protein Bcl2, enhanced cell killing after PDT. PDT-induced accumulation of ceramide in the endoplasmic reticulum and mitochondria was inhibited by FB. PDT-induced Bax translocation to the mitochondria and cytochrome c release were also inhibited by FB. These novel data suggest that PDT-induced cell death via apoptosis is CERS/ceramide-dependent. † Electronic supplementary information (ESI) available. See</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="49e2c09590d5c5267fb2105ccabc8ea3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223491,"asset_id":107598977,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223491/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598977"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598977"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598977; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598976"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598976/Interaction_of_acid_ceramidase_inhibitor_LCL521_with_tumor_response_to_photodynamic_therapy_and_photodynamic_therapy_generated_vaccine"><img alt="Research paper thumbnail of Interaction of acid ceramidase inhibitor LCL521 with tumor response to photodynamic therapy and photodynamic therapy-generated vaccine" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598976/Interaction_of_acid_ceramidase_inhibitor_LCL521_with_tumor_response_to_photodynamic_therapy_and_photodynamic_therapy_generated_vaccine">Interaction of acid ceramidase inhibitor LCL521 with tumor response to photodynamic therapy and photodynamic therapy-generated vaccine</a></div><div class="wp-workCard_item"><span>International Journal of Cancer</span><span>, May 18, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Acid ceramidase has been identified as a promising target for cancer therapy. One of its most eff...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Acid ceramidase has been identified as a promising target for cancer therapy. One of its most effective inhibitors, LCL521, was examined as adjuvant to photodynamic therapy (PDT) using mouse squamous cell carcinoma SCCVII model of head and neck cancer. Lethal effects of PDT, assessed by colony forming ability of in vitro treated SCCVII cells, were greatly enhanced when combined with 10 µM LCL521 treatment particularly when preceding PDT. When PDT-treated SCCVII cells are used to vaccinate SCCVII tumor-bearing mice (PDT vaccine protocol), adjuvant LCL521 treatment (75 mg/kg) resulted in a marked retardation of tumor growth. This effect can be attributed to the capacity of LCL521 to effectively restrict the activity of two main immunoregulatory cell populations (Tregs and myeloid-derived suppressor cells, MDSCs) that are known to hinder the efficacy of PDT vaccines. The therapeutic benefit with adjuvant LCL521 was also achieved with SCCVII tumors treated with standard PDT when using immunocompetent mice but not with immunodeficient hosts. The interaction of LCL521 with PDT-based antitumor mechanisms is dominated by immune system contribution that includes overriding the effects of immunoregulatory cells, but could also include a tacit contribution from boosting direct tumor cell kill. Acid ceramidase, one of the key enzymes in sphingolipid metabolism, has been identified as a promising target for cancer therapy. Using a head and neck cancer mouse model, here the authors examine the effectiveness of acid ceramidase inhibitor LCL521 as adjuvant to photodynamic therapy (PDT). They show that targeting acid ceramidase can be successfully exploited in combined treatments with PDT or PDT-generated vaccines. LCL521 treatment enhances direct lethal effects initiated by PDT-induced oxidative stress responses in vitro. In vivo, the hindering effects of major immunoregulatory populations following PDT of tumors or related vaccine protocols can be restrained by LCL521 treatment. This article is protected by copyright. All rights reserved.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598976"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598976"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598976; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598976]").text(description); $(".js-view-count[data-work-id=107598976]").attr('title', description).tooltip(); 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</script> <div class="js-work-strip profile--work_container" data-work-id="107598975"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598975/Controlling_Immunoregulatory_Cell_Activity_for_Effective_Photodynamic_Therapy_of_Cancer"><img alt="Research paper thumbnail of Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598975/Controlling_Immunoregulatory_Cell_Activity_for_Effective_Photodynamic_Therapy_of_Cancer">Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer</a></div><div class="wp-workCard_item"><span>Springer eBooks</span><span>, 2022</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598975"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598975"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598975; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598975]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598975,"title":"Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer","internal_url":"https://www.academia.edu/107598975/Controlling_Immunoregulatory_Cell_Activity_for_Effective_Photodynamic_Therapy_of_Cancer","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598974"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598974/C6_pyridinium_ceramide_sensitizes_SCC17B_human_head_and_neck_squamous_cell_carcinoma_cells_to_photodynamic_therapy"><img alt="Research paper thumbnail of C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy" class="work-thumbnail" src="https://attachments.academia-assets.com/106223488/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598974/C6_pyridinium_ceramide_sensitizes_SCC17B_human_head_and_neck_squamous_cell_carcinoma_cells_to_photodynamic_therapy">C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Feb 1, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Combining photodynamic therapy (PDT) 1 with another anticancer treatment modality is an important...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Combining photodynamic therapy (PDT) 1 with another anticancer treatment modality is an important strategy for improved efficacy. PDT with Pc4, a silicon phthalocyanine photosensitizer, was combined with C6-pyridinium ceramide (LCL29) to determine their potential to promote death of SCC17B human head and neck squamous cell carcinoma cells. PDT+LCL29-induced enhanced cell death was inhibited by zVAD-fmk, a pan-caspase inhibitor, and fumonisin B1 (FB), a ceramide synthase inhibitor. Quantitative confocal microscopy showed that combining PDT with LCL29 enhanced FB-sensitive ceramide accumulation in the mitochondria. Furthermore, PDT +LCL29 induced enhanced FB-sensitive redistribution of cytochrome c and caspase-3 activation. Overall, the data indicate that PDT+LCL29 enhanced cell death via FB-sensitive, mitochondrial ceramide accumulation and apoptosis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f16c6e6cb10591d84969252f81e15761" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223488,"asset_id":107598974,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223488/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598974"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598974"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598974; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598974]").text(description); $(".js-view-count[data-work-id=107598974]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598974; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598974']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f16c6e6cb10591d84969252f81e15761" } } $('.js-work-strip[data-work-id=107598974]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598974,"title":"C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy","internal_url":"https://www.academia.edu/107598974/C6_pyridinium_ceramide_sensitizes_SCC17B_human_head_and_neck_squamous_cell_carcinoma_cells_to_photodynamic_therapy","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[{"id":106223488,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223488/thumbnails/1.jpg","file_name":"pmc4662378.pdf","download_url":"https://www.academia.edu/attachments/106223488/download_file","bulk_download_file_name":"C6_pyridinium_ceramide_sensitizes_SCC17B.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223488/pmc4662378-libre.pdf?1696453837=\u0026response-content-disposition=attachment%3B+filename%3DC6_pyridinium_ceramide_sensitizes_SCC17B.pdf\u0026Expires=1740033853\u0026Signature=C-P1hglN5arxwtSH-WLu4jo2Xw06MP5UqD66wX5RX8hlTyFxIUnztBsp8YYDsV0aMftxdhGpymKHECR75XgWkHxCxAyAAdyi1le4VzeCvVibbrbJEOfn8CdnA4jS2Ad7Lc8fV5TvSRJIg2L-tuvUfBH4Yj1vsnKpHixBB5nOKg7lXgvWVzAIqP2aJqWqpE9z6pIYOsJrieGieHlkz-7WlezUG8Nf2bU0YC40iwZ4y7dF85KwcoBvWYY0ErRcnCu6Ek~GP3uZ97wsyyvBSWlVUQDKkOJZt5jooL6LE~9dboB9Xk8vXS7DLme-To2WZYY7Eb-WLMeVxwXpmNllFlvUwQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"},{"id":106223489,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223489/thumbnails/1.jpg","file_name":"pmc4662378.pdf","download_url":"https://www.academia.edu/attachments/106223489/download_file","bulk_download_file_name":"C6_pyridinium_ceramide_sensitizes_SCC17B.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223489/pmc4662378-libre.pdf?1696453838=\u0026response-content-disposition=attachment%3B+filename%3DC6_pyridinium_ceramide_sensitizes_SCC17B.pdf\u0026Expires=1740033853\u0026Signature=LwZf2sso-Bj-Jg3zGG2HMNLlNt-ercp8UfsVJMSaW~QIJE9K9oeg~XKglAth2dcexMQJSR~YjjuER2pNOLAXCiX-XdKq1rv80xtZhrLX4M0zYP~-Wk19khepvMb59LnnrZ6aeIaW9K9wfgcryFtuPMFEYzik1~iu4vaNzFeQtCvWWBfAF8a~q9EohU0Bli49RGjuScOp2WoZzQEvype7-NRWGfoImGMZjBpY7SSNHU7cnwMmtYY0DGdJ~qMUpEG-zUJQDu3sd4vAOVPZbBsXQ-y-fiIO7RQS8dKMqTeqUP73ALKQCTMXpwSFYHpqU7UK9rQ7dYyg1Os7gHtbpmk~iw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598973"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598973/Increased_tumour_dihydroceramide_production_after_Photofrin_PDT_alone_and_improved_tumour_response_after_the_combination_with_the_ceramide_analogue_LCL29_Evidence_from_mouse_squamous_cell_carcinomas"><img alt="Research paper thumbnail of Increased tumour dihydroceramide production after Photofrin-PDT alone and improved tumour response after the combination with the ceramide analogue LCL29. Evidence from mouse squamous cell carcinomas" class="work-thumbnail" src="https://attachments.academia-assets.com/106223487/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598973/Increased_tumour_dihydroceramide_production_after_Photofrin_PDT_alone_and_improved_tumour_response_after_the_combination_with_the_ceramide_analogue_LCL29_Evidence_from_mouse_squamous_cell_carcinomas">Increased tumour dihydroceramide production after Photofrin-PDT alone and improved tumour response after the combination with the ceramide analogue LCL29. Evidence from mouse squamous cell carcinomas</a></div><div class="wp-workCard_item"><span>British Journal of Cancer</span><span>, Feb 1, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Photodynamic therapy (PDT) has been proven effective for treatment of several types of cancer. Ph...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Photodynamic therapy (PDT) has been proven effective for treatment of several types of cancer. Photodynamic therapy alone, however, attains limited cures with some tumours and there is need for its improved efficacy in such cases. Sphingolipid (SL) analogues can promote tumour response in combination with anticancer drugs. In this study, we used mouse SCCVII squamous cell carcinoma tumours to determine the impact of Photofrin-PDT on the in vivo SL profile and the effect of LCL29, a C6-pyridinium ceramide, on PDT tumour response. Following PDT, the levels of dihydroceramides (DHceramides), in particular C20-DHceramide, were elevated in tumours. Similarly, increases in DHceramides, in addition to C20:1-ceramide, were found in PDT-treated SCCVII cells. These findings indicate the importance of the de novo ceramide pathway in Photofrin-PDT response not only in cells but also in vivo. Notably, co-exposure of SCCVII tumours to Photofrin-PDT and LCL29 led to enhanced tumour response compared with PDT alone. Thus, we show for the first time that Photofrin-PDT has a distinct signature effect on the SL profile in vitro and in vivo, and that the combined treatment advances PDT therapeutic gain, implying translational significance of the combination.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4f9abcf283dceab3982f9fd20437ea12" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223487,"asset_id":107598973,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223487/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598973"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598973"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598973; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598973]").text(description); $(".js-view-count[data-work-id=107598973]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598973; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598973']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4f9abcf283dceab3982f9fd20437ea12" } } $('.js-work-strip[data-work-id=107598973]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598973,"title":"Increased tumour dihydroceramide production after Photofrin-PDT alone and improved tumour response after the combination with the ceramide analogue LCL29. Evidence from mouse squamous cell carcinomas","internal_url":"https://www.academia.edu/107598973/Increased_tumour_dihydroceramide_production_after_Photofrin_PDT_alone_and_improved_tumour_response_after_the_combination_with_the_ceramide_analogue_LCL29_Evidence_from_mouse_squamous_cell_carcinomas","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[{"id":106223487,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223487/thumbnails/1.jpg","file_name":"6604896.pdf","download_url":"https://www.academia.edu/attachments/106223487/download_file","bulk_download_file_name":"Increased_tumour_dihydroceramide_product.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223487/6604896-libre.pdf?1696453829=\u0026response-content-disposition=attachment%3B+filename%3DIncreased_tumour_dihydroceramide_product.pdf\u0026Expires=1740033853\u0026Signature=Rulh6jVQA03fs1jcHdSsI~IPcu5R8HipnDOp55dSiP~vDlIvIZjYiiQcPAkhvw8-AH6WSTYU509XJSxN4pt3g8rucoflWTHXzlH8CRNcxHRcnCXEjeoOGTnM-3BmZ7Zu3OjIkOMabgRsVVMQE2ay18H~nQ1aU3UCp~wOwE39uOL81jEomRoCk5si8dVHiN0rFTQLVL296pEujzBOFel77pL88NpP0hjGCNygmK2M4idv06Gi3z48eYtCcdU7Ju1vV74bvw2WgY~spHpo18Yd8kjL0Seiss6NOlQFAOfK9S~Z-N9V4yy~aPDX2waNdQK2-2y-B0FHbhQO2KQWO4D0xQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598972"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598972/Characteristics_of_complement_activation_in_mice_bearing_Lewis_lung_carcinomas_treated_by_photodynamic_therapy"><img alt="Research paper thumbnail of Characteristics of complement activation in mice bearing Lewis lung carcinomas treated by photodynamic therapy" class="work-thumbnail" src="https://attachments.academia-assets.com/106223511/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598972/Characteristics_of_complement_activation_in_mice_bearing_Lewis_lung_carcinomas_treated_by_photodynamic_therapy">Characteristics of complement activation in mice bearing Lewis lung carcinomas treated by photodynamic therapy</a></div><div class="wp-workCard_item"><span>Cancer Letters</span><span>, Jul 1, 2005</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Following treatment of Lewis lung carcinomas (LLC) by Photofrin-mediated photodynamic therapy (PD...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Following treatment of Lewis lung carcinomas (LLC) by Photofrin-mediated photodynamic therapy (PDT), tumor tissues and sera of host mice were collected for the analysis of complement activity. Elevated tumor C3 levels were detected between 1 and 24 h after PDT, while serum C3 levels increased significantly at 24 h post therapy. Increased alternative complement pathway activity in the serum was evident between 1 and 3 days post PDT. Blocking C3a-or C5a-receptors in the host mice decreased the efficacy of PDT in producing LLC tumor cures, supporting the importance of complement action in PDT-mediated tumor destruction.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="860be353d06cc0ec9bcfc81166cb54a4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223511,"asset_id":107598972,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223511/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598972"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598972"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598972; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598971"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598971/Fumonisin_B1_Inhibits_Endoplasmic_Reticulum_Stress_Associated_apoptosis_After_FoscanPDT_Combined_with_C6_Pyridinium_Ceramide_or_Fenretinide"><img alt="Research paper thumbnail of Fumonisin B1 Inhibits Endoplasmic Reticulum Stress Associated-apoptosis After FoscanPDT Combined with C6-Pyridinium Ceramide or Fenretinide" class="work-thumbnail" src="https://attachments.academia-assets.com/106223485/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598971/Fumonisin_B1_Inhibits_Endoplasmic_Reticulum_Stress_Associated_apoptosis_After_FoscanPDT_Combined_with_C6_Pyridinium_Ceramide_or_Fenretinide">Fumonisin B1 Inhibits Endoplasmic Reticulum Stress Associated-apoptosis After FoscanPDT Combined with C6-Pyridinium Ceramide or Fenretinide</a></div><div class="wp-workCard_item"><span>Anticancer Research</span><span>, Feb 10, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Colorectal cancer is the third leading cause of cancer-related mortality in most deve...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Colorectal cancer is the third leading cause of cancer-related mortality in most developed countries. This mortality is mainly due to the metastatic progression to the liver with frequent recurrence. Colorectal cancer remains a therapeutic challenge and this has intensified the search for new drug targets. In an effort to establish a novel targeted-therapy, we studied the molecular mechanisms of cancer stem cell inhibitor salinomycin. Materials and Methods: Co-immunoprecipitation was performed to examine STAT3-STAT1 protein interactions. Telomerase activity was measured by polymerase chain reaction (PCR) and ELISA assays. Apoptosis and cell stress arrays were analyzed to identify key proteins responding to salinomycin treatments. Results: IL-6 and TNF-α induced STAT3 and STAT1 interactions, however the interactions were abolished by salinomycin challenge. Salinomycin reduced cancer stem cell phenotype and decreased telomerase activity of colorectal cancer cells. Conclusion: Our work uncovers a new mechanism through which salinomycin inhibits cancer stemness suggesting a novel targeted-therapy for metastatic colorectal cancer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7f0157ded8d4b8a33e3884776d993910" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223485,"asset_id":107598971,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223485/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598971"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598971"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598971; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598971]").text(description); $(".js-view-count[data-work-id=107598971]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598971; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598971']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598970"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598970/Enhanced_apoptotic_cancer_cell_killing_after_Foscan_photodynamic_therapy_combined_with_fenretinide_via_de_novo_sphingolipid_biosynthesis_pathway"><img alt="Research paper thumbnail of Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway" class="work-thumbnail" src="https://attachments.academia-assets.com/106223490/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598970/Enhanced_apoptotic_cancer_cell_killing_after_Foscan_photodynamic_therapy_combined_with_fenretinide_via_de_novo_sphingolipid_biosynthesis_pathway">Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Jun 1, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We and others have shown that stresses, including photodynamic therapy (PDT) 1 , can disrupt the ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We and others have shown that stresses, including photodynamic therapy (PDT) 1 , can disrupt the de novo sphingolipid biosynthesis pathway, leading to changes in the levels of sphingolipids, and subsequently, modulation of cell death. The de novo sphingolipid biosynthesis pathway includes a ceramide synthase-dependent reaction, giving rise to dihydroceramide, which is then converted in a desaturase-dependent reaction to ceramide. In this study we tested the hypothesis that combining Foscan-mediated PDT with desaturase inhibitor fenretinide (HPR) enhances cancer cell killing. We discovered that by subjecting SCC19 cells, a human head and neck squamous cell carcinoma cell line, to PDT+HPR resulted in enhanced accumulation of C16-dihydroceramide, not ceramide. Concomitantly, mitochondrial depolarization was enhanced by the combined treatment. Enhanced activation of caspase-3 after PDT +HPR was inhibited by FB. Enhanced clonogenic cell death after the combination was sensitive to FB, as well as Bcl2-and caspase inhibitors. Treatment of mouse SCCVII squamous cell carcinoma tumors with PDT+HPR resulted in improved long-term tumor cures. Overall, our data showed that combining PDT with HPR enhanced apoptotic cancer cell killing and antitumor efficacy of PDT. The data suggest the involvement of the de novo sphingolipid biosynthesis pathway in enhanced apoptotic cell killing after PDT+HPR, identify PDT+HPR as a more effective combination than either treatment alone, and that the combination has potential for cancer treatment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f0a1c466b649c3b68d26756b67f30e50" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223490,"asset_id":107598970,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223490/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598970"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598970"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598970; 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window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598968]").text(description); $(".js-view-count[data-work-id=107598968]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598968; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598968']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598967"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598967/Mechanistic_insights_into_ceramidase_inhibitor_LCL521_enhanced_tumor_cell_killing_by_photodynamic_and_thermal_ablation_therapies"><img alt="Research paper thumbnail of Mechanistic insights into ceramidase inhibitor LCL521-enhanced tumor cell killing by photodynamic and thermal ablation therapies" class="work-thumbnail" src="https://attachments.academia-assets.com/106223507/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598967/Mechanistic_insights_into_ceramidase_inhibitor_LCL521_enhanced_tumor_cell_killing_by_photodynamic_and_thermal_ablation_therapies">Mechanistic insights into ceramidase inhibitor LCL521-enhanced tumor cell killing by photodynamic and thermal ablation therapies</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, Sep 1, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Our recent investigation uncovered that the acid ceramidase inhibitor LCL521 enhances the direct ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Our recent investigation uncovered that the acid ceramidase inhibitor LCL521 enhances the direct tumor cell killing effect of photodynamic therapy (PDT) treatment. The present study aimed at elucidating the mechanisms underlying this effect. Exposing mouse squamous cell carcinoma SCCVII cells treated with temoporfin-based PDT to LCL521 (rising ceramide concentration) produced a much greater decrease in cell survival than comparable exposure to the sphingosine kinase-1 inhibitor PF543 (that reduces sphingosine-1-phosphate concentration). This is consistent with recognizing the rising levels of pro-apoptotic sphingolipid ceramide as being more critical in promoting the death of PDT-treated cells than the reduction in the availability of pro-survival acting sphingosine-1 phosphate. This pro-apoptotic impact of LCL521, which was suppressed by the apoptosis inhibitor bongkrekic acid, involves the interaction with the cellular stress signaling network. Hence, inhibiting the key elements of these pathways markedly influenced the adjuvant effect of LCL521 on the PDT response. Particularly effective was the inositolrequiring element-1 (IRE1) kinase inhibitor STF-083010 that dramatically enhanced the killing of cells treated with PDT plus LCL521. An important role in the survival of these cells was exhibited by master transcription factors STAT3 and HIF-1α. The STAT3 inhibitor NSC 74859 was especially effective in further reducing the cell survival rates, suggesting its possible exploitation for therapeutic gain. An additional finding in this study is that LCL521-promoted PDT-mediated cell killing through ceramide-mediated lethal effects is extended to the interaction with other cancer treatment modalities with a rapid cellular stress impact such as photothermal therapy (PTT) and cryoablation therapy (CAT).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="917b7ef79340a824f7d87a3c66456346" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223507,"asset_id":107598967,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223507/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598967"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598967"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598967; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598966"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598966/Drug_delivery_technologies_and_immunological_aspects_of_photodynamic_therapy"><img alt="Research paper thumbnail of Drug delivery technologies and immunological aspects of photodynamic therapy" class="work-thumbnail" src="https://attachments.academia-assets.com/106223505/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598966/Drug_delivery_technologies_and_immunological_aspects_of_photodynamic_therapy">Drug delivery technologies and immunological aspects of photodynamic therapy</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, May 1, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="619b43fbe37bdeec712277bac4700fba" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223505,"asset_id":107598966,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223505/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598966"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598966"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598966; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598965"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598965/Enhancement_of_tumour_response_to_photodynamic_therapy_by_adjuvant_mycobacterium_cell_wall_treatment"><img alt="Research paper thumbnail of Enhancement of tumour response to photodynamic therapy by adjuvant mycobacterium cell-wall treatment" class="work-thumbnail" src="https://attachments.academia-assets.com/106223508/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598965/Enhancement_of_tumour_response_to_photodynamic_therapy_by_adjuvant_mycobacterium_cell_wall_treatment">Enhancement of tumour response to photodynamic therapy by adjuvant mycobacterium cell-wall treatment</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Jul 1, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mycobacterium cell-wall extract (MCWE) is a potent non-specific immunostimulant that elicits a lo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mycobacterium cell-wall extract (MCWE) is a potent non-specific immunostimulant that elicits a local inflammatory response associated with antitumour activity. Tumour-localized administration of MCWE has been examined as an adjuvant to photodynamic therapy (PDT) mediated by the photosensitizers Photofrin, benzoporphyrin derivative monoacid (BPD), metatetrahydroxyphenylchlorin (mTHPC), or zinc (II)-phthaIocyanine (ZnPc). A single MCWE treatment, given immediately after light treatment of murine EMT6 tumours, potentiates the curative effect of PDT. A similar enhancement of tumour response to Photofrin-based PDT is obtained with the live Bacillus Calmerte-Guhrin (BCG) vaccine. Despite differences in the kinetics/intensity of damage induction to tumour microvasculature and othercharacteristics underlying the mechanism of antitumour activity of Photofrin, BPD, mTHPC and ZnPc, there appear to be no marked differences in the therapeutic benefit of adjuvant MCWE therapy combined with the PDT mediated by these various photosensitizers. This may be related to the fact that MCWE elicits a wide range of immunomodulatory effects that could amplify and sustain the inflammatory/immune responses triggered by PDT. The enhancement of inflammatory effector cell activity is indicated by the increased infiltration of neutrophils and other myeloid cells at the expense of malignant cells found in the MCWE plus mTHPC-based PDT treatment group compared to the PDT-only group.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="860c4a2618c637410b403515817e9e61" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223508,"asset_id":107598965,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223508/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598965"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598965"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598965; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598964"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598964/Induction_of_Systemic_Neutrophil_Response_in_Mice_by_Photodynamic_Therapy_of_Solid_Tumors_"><img alt="Research paper thumbnail of Induction of Systemic Neutrophil Response in Mice by Photodynamic Therapy of Solid Tumors¶" class="work-thumbnail" src="https://attachments.academia-assets.com/106223516/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598964/Induction_of_Systemic_Neutrophil_Response_in_Mice_by_Photodynamic_Therapy_of_Solid_Tumors_">Induction of Systemic Neutrophil Response in Mice by Photodynamic Therapy of Solid Tumors¶</a></div><div class="wp-workCard_item"><span>Photochemistry and Photobiology</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Photodynamic therapy (PDT) of solid tumors elicits a strong, acute inflammatory response characte...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Photodynamic therapy (PDT) of solid tumors elicits a strong, acute inflammatory response characterized by a rapid and massive infiltration of activated neutrophils into the tumor. The present study investigated the impact of PDT on the systemic and local (treatment site) kinetics of neutrophil trafficking and activity in mouse SCCVII and EMT6 tumor models. Differential leukocyte counts in the peripheral blood of treated mice revealed a pronounced neutrophilia developing rapidly after Photofrin porfimer sodium (Photofrin)-or tetra(m-tetrahydroxyphenyl)chlorin (mTHPC)-based PDT. Significant neutrophilia was also observed upon PDT treatment of normal dorsal skin but not on the footpad of tumor-free mice. The changes in circulating neutrophil numbers were accompanied by an efflux of these cells from the bone marrow. An increased proportion of cells with high L-selectin (CD62L antigen) expression was found among bone-marrow-residing neutrophils 6-24 h after PDT, and in neutrophils in the peripheral circulation and treated tumors 24 h after therapy. Complement inhibition completely prevented the development of PDT-induced neutrophilia. The results of the present study demonstrate that treatment of solid tumors by PDT induces a strong and protracted increase in systemic neutrophil numbers mediated by complement activation. This reaction reflects rapid and massive mobilization and activation of neutrophils for the destruction of PDT-treated tumor tissue.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="83cfe4f7f58e2489ca03e9fd71567ff5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223516,"asset_id":107598964,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223516/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598964"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598964"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598964; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598963"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598963/Neutrophils_as_inflammatory_and_immune_effectors_in_photodynamic_therapy_treated_mouse_SCCVII_tumours"><img alt="Research paper thumbnail of Neutrophils as inflammatory and immune effectors in photodynamic therapy-treated mouse SCCVII tumours" class="work-thumbnail" src="https://attachments.academia-assets.com/106223506/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598963/Neutrophils_as_inflammatory_and_immune_effectors_in_photodynamic_therapy_treated_mouse_SCCVII_tumours">Neutrophils as inflammatory and immune effectors in photodynamic therapy-treated mouse SCCVII tumours</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, Jul 16, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Neutrophils have become recognised as important contributors to the effectiveness of tumour eradi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Neutrophils have become recognised as important contributors to the effectiveness of tumour eradication by photodynamic therapy (PDT). In this study, we have used the mouse SCCVII squamous cell carcinoma model to investigate the activity of neutrophils in tumours treated by PDT. Tumour levels of neutrophilic myeloperoxidase (MPO) demonstrated not only a massive and sustained sequestration of these cells in PDT-treated tumours but also revealed their activated state evidenced by the presence of released MPO. Among the adhesion molecules expressed on tumour vascular endothelium, ICAM-1 appears to be of primary importance in the invasion of neutrophils into PDT-treated tumours, because its functional blocking with monoclonal antibodies reduced the tumour cure rate. A marked upregulation of its ligands CD11b/CD18 and CD11c/CD18 found on neutrophils associated with PDTtreated tumours supports this assumption. To evaluate the role of inflammatory cytokines regulating neutrophil activity, neutralising antibodies were given to mice before PDT treatment. The results suggest that IL-1β activity is critical for the therapeutic outcome, since its neutralisation diminished the cure rates of PDT-treated tumours. No significant effect was observed with anti-IL-6 and anti-TNF-α treatment. Further flow cytometry-based examination of neutrophils found in PDT-treated tumours revealed that these cells express MHC class II molecules, which suggests their engagement as antigen-presenting cells and involvement in the development of antitumour immune response.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="611484edb3c5d985e42b26bf696d6268" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223506,"asset_id":107598963,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223506/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598963"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598963"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598963; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="3310859" id="papers"><div class="js-work-strip profile--work_container" data-work-id="107598982"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598982/Cationic_ceramides_and_analogues_LCL30_and_LCL85_as_adjuvants_to_photodynamic_therapy_of_tumors"><img alt="Research paper thumbnail of Cationic ceramides and analogues, LCL30 and LCL85, as adjuvants to photodynamic therapy of tumors" class="work-thumbnail" src="https://attachments.academia-assets.com/106223493/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598982/Cationic_ceramides_and_analogues_LCL30_and_LCL85_as_adjuvants_to_photodynamic_therapy_of_tumors">Cationic ceramides and analogues, LCL30 and LCL85, as adjuvants to photodynamic therapy of tumors</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Sep 1, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Photodynamic therapy (PDT) is known to alter the expression of various genes in treated cells. Th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Photodynamic therapy (PDT) is known to alter the expression of various genes in treated cells. This prompted us to examine the activity of genes encoding two important enzymes in sphingolipid (SL) metabolism, dihydroceramide desaturase (DES) and sphingosine kinase (SPHK), in mouse SCCVII tumor cells treated by PDT using either the porphyrin-based photosensitizer Photofrin or silicon phthalocyanine Pc4. The results revealed that PDT induced an upregulation in the expression of two major isoforms of both genes (DES1 and DES2 as well as SPHK1 and SPHK2). While the changes were generally moderate (2-3 fold gains), the increase in DES2 expression was more pronounced and it was much greater with Photofrin-PDT than with Pc4-PDT (over 23-fold vs. less than 5-fold). Combining either Photofrin-PDT or Pc4-PDT with the cationic C16-ceramide LCL30 (20 mg/kg i.p.) for treatment of subcutaneously growing SCCVII tumors rendered important differences in the therapy outcome. Photofrin-PDT, used at a dose that attained good initial response but no tumor cures, produced 50% cures when combined with a single LCL30 treatment. In contrast, the same LCL30 treatment combined with Pc4-PDT had no significant effect on tumor response. The optimal timing of LCL30 injection was immediately after Photofrin-PDT. The therapeutic benefit was lost when LCL30 was given in two 20 mg/kg injections encompassing intervals before and after PDT. LCL85, the cationic B13 ceramide analogue and SL-modulating agent, also increased cure rates of Photofrin-PDT treated tumors, but the therapeutic benefit was less pronounced than with LCL30. These results with LCL30 and LCL85, and our previous findings for LCL29 (another SL analogue), assert the potential of SLs for use as adjuvants to augment the efficacy of PDT-mediated tumor destruction.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="28685846537bb02a0ceadab21b0df964" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223493,"asset_id":107598982,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223493/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598982"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598982"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598982; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598981"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598981/Antitumor_Efficacy_of_Photodynamic_Therapy_Using_Novel_Nanoformulations_of_Hypocrellin_Photosensitizer_SL052"><img alt="Research paper thumbnail of Antitumor Efficacy of Photodynamic Therapy Using Novel Nanoformulations of Hypocrellin Photosensitizer SL052" class="work-thumbnail" src="https://attachments.academia-assets.com/106223510/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598981/Antitumor_Efficacy_of_Photodynamic_Therapy_Using_Novel_Nanoformulations_of_Hypocrellin_Photosensitizer_SL052">Antitumor Efficacy of Photodynamic Therapy Using Novel Nanoformulations of Hypocrellin Photosensitizer SL052</a></div><div class="wp-workCard_item"><span>Photochemistry and Photobiology</span><span>, Dec 20, 2011</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Recent preclinical and clinical testing of hypocrellin-based photosensitizer SL052 for use in pho...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Recent preclinical and clinical testing of hypocrellin-based photosensitizer SL052 for use in photodynamic therapy (PDT) of cancer has shown encouraging results. Further optimization of its formulation for delivery could considerably extend the therapeutic efficiency of this drug. A nanoformulation encapsulating SL052 into biodegradable polymer poly(lactic-co-glycolic acid) (PLGA) was developed using a single-emulsion solvent evaporation technique and characterized in terms of particle size and loading of the photosensitizing agent. This nanoformulation, SL052-PLGA-nanoparticles (NPs), was compared with recently created nanoformulation based on polyvinylpyrrolidone (SL052-PVP-NPs) and standard liposomal SL052 preparation in terms of efficacy when used for PDT treatment of squamous cell carcinomas SCCVII growing subcutaneously in syngeneic mice. The therapeutic effect of PDT using these three different SL052 formulations was tested for both 1 and 4 h intervals between drug injection and tumor light exposure. The longer time interval produced higher tumor cure rates with all SL052 preparations. With both drug-light intervals, PDT based on SL052-PLGA-NPs produced superior therapeutic benefit compared with the other two SL052 formulations.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f879f2e94b041ce07544a38f6b2993f5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223510,"asset_id":107598981,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223510/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598981"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598981"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598981; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598980"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598980/Increased_killing_of_SCCVII_squamous_cell_carcinoma_cells_after_the_combination_of_Pc_4_photodynamic_therapy_and_dasatinib_is_associated_with_enhanced_caspase_3_activity_and_ceramide_synthase_1_upregulation"><img alt="Research paper thumbnail of Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598980/Increased_killing_of_SCCVII_squamous_cell_carcinoma_cells_after_the_combination_of_Pc_4_photodynamic_therapy_and_dasatinib_is_associated_with_enhanced_caspase_3_activity_and_ceramide_synthase_1_upregulation">Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation</a></div><div class="wp-workCard_item"><span>International Journal of Oncology</span><span>, Oct 9, 2013</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598980"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598980"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598980; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598980]").text(description); $(".js-view-count[data-work-id=107598980]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598980; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598980']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598980]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598980,"title":"Increased killing of SCCVII squamous cell carcinoma cells after the combination of Pc 4 photodynamic therapy and dasatinib is associated with enhanced caspase-3 activity and ceramide synthase 1 upregulation","internal_url":"https://www.academia.edu/107598980/Increased_killing_of_SCCVII_squamous_cell_carcinoma_cells_after_the_combination_of_Pc_4_photodynamic_therapy_and_dasatinib_is_associated_with_enhanced_caspase_3_activity_and_ceramide_synthase_1_upregulation","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598979"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598979/Enhancement_of_laser_cancer_treatment_by_a_chitosan_derived_immunoadjuvant"><img alt="Research paper thumbnail of Enhancement of laser cancer treatment by a chitosan-derived immunoadjuvant" class="work-thumbnail" src="https://attachments.academia-assets.com/106223522/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598979/Enhancement_of_laser_cancer_treatment_by_a_chitosan_derived_immunoadjuvant">Enhancement of laser cancer treatment by a chitosan-derived immunoadjuvant</a></div><div class="wp-workCard_item"><span>Photochemistry and Photobiology</span><span>, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A chitosan derivative, glycated chitosan (GC), has been used as an immunostimulant for cancer tre...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A chitosan derivative, glycated chitosan (GC), has been used as an immunostimulant for cancer treatment in laser immunotherapy. The function of GC is to enhance the host immune response after direct cancer cell destruction by a selective laser photothermal interaction. To further test its effects, laser immunotherapy was extended to include several different adjuvants for immunological stimulation and to include photodynamic therapy (PDT) as a different tumordestruction mechanism. Complete Freund (CF) adjuvant, incomplete Freund (IF) adjuvant and Corynebucterium parvum (CP) were selected for treatment of metastatic mammary tumors in rats, in combination with a selective photothermal interaction. The solution of the immunoadjuvants admixed with indocyanine green (ICG), a light-absorbing dye, was injected directly into the tumors, followed by noninvasive irradiation of an 805 nm laser. Combined with PDT, in the treatment of tumors in mice, GC was administered peritumorally immediately after laser irradiation. The survivals of treated animals were compared with untreated control animals. In the treatment of rat tumors, CF, IF and CP raised the cure rates from 0% to 18%, 7% and 9%, respectively. In comparison, GC resulted in a 29% long-term survival. In the treatment of EMT6 mammary sarcoma in mice, GC of 0.5% and 1.5% concentrations increased the cure rates of Photofrin-based PDT treatment from 38% to 63% and 75%, respectively. In the treatment of Line 1 lung adenocarcinoma in mice, a 1.67% GC solution enabled a noncurative mesosubstituted tetra(meta-hydroxy-pheny1)chlorin-based PDT to YPosted on the website on 9 November 2004.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="266b1a3f0f119b9ebc36de1f94fce810" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223522,"asset_id":107598979,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223522/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598979"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598979"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598979; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "266b1a3f0f119b9ebc36de1f94fce810" } } $('.js-work-strip[data-work-id=107598979]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598979,"title":"Enhancement of laser cancer treatment by a chitosan-derived immunoadjuvant","internal_url":"https://www.academia.edu/107598979/Enhancement_of_laser_cancer_treatment_by_a_chitosan_derived_immunoadjuvant","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[{"id":106223522,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223522/thumbnails/1.jpg","file_name":"j.1751-1097.2005.tb01541.x20231004-1-9surwd.pdf","download_url":"https://www.academia.edu/attachments/106223522/download_file","bulk_download_file_name":"Enhancement_of_laser_cancer_treatment_by.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223522/j.1751-1097.2005.tb01541.x20231004-1-9surwd-libre.pdf?1696453825=\u0026response-content-disposition=attachment%3B+filename%3DEnhancement_of_laser_cancer_treatment_by.pdf\u0026Expires=1740033853\u0026Signature=CAnVHKacvv~TWY8Cv7mOtftvCebEnjSXa-mLnqITRiRas3e0VpqM5N1n~n7QLmfF~oUjgQnmxrn01UVXJpUDUOQfkrJMN30kDy59BaZ3OSAnVSh2igrqo9O7rqABu-SEUV-6BKb8o3VNqFlJzmJI3FNAHDrWw0195QXuvWcFki6KDJ4bSJNfucOzIKQL5XMdDAo6mFgQFdnX2Zdo9lfwqSRKt-5pcxzyXIN0cds7gdHEoQYNYBYCb1u3-gaQ86Em4GFPUXgoqrDcw7XEtdSgNTlSeeer46H6DtuYbY7dr6l-9sTRemFKzn8dYno24qfzzjUgNpW8~n2eGL-PZdUAIA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598978"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598978/_title_Laser_immunotherapy_a_novel_approach_for_metastatic_tumors_title_"><img alt="Research paper thumbnail of <title>Laser immunotherapy: a novel approach for metastatic tumors</title>" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598978/_title_Laser_immunotherapy_a_novel_approach_for_metastatic_tumors_title_"><title>Laser immunotherapy: a novel approach for metastatic tumors</title></a></div><div class="wp-workCard_item"><span>SPIE Proceedings</span><span>, Aug 20, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598978"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598978"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598978; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598978]").text(description); $(".js-view-count[data-work-id=107598978]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598978; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598978']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598978]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598978,"title":"\u003ctitle\u003eLaser immunotherapy: a novel approach for metastatic tumors\u003c/title\u003e","internal_url":"https://www.academia.edu/107598978/_title_Laser_immunotherapy_a_novel_approach_for_metastatic_tumors_title_","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598977"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598977/Ceramide_synthase_inhibitor_fumonisin_B1_inhibits_apoptotic_cell_death_in_SCC17B_human_head_and_neck_squamous_carcinoma_cells_after_Pc4_photosensitization"><img alt="Research paper thumbnail of Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization" class="work-thumbnail" src="https://attachments.academia-assets.com/106223491/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598977/Ceramide_synthase_inhibitor_fumonisin_B1_inhibits_apoptotic_cell_death_in_SCC17B_human_head_and_neck_squamous_carcinoma_cells_after_Pc4_photosensitization">Ceramide synthase inhibitor fumonisin B1 inhibits apoptotic cell death in SCC17B human head and neck squamous carcinoma cells after Pc4 photosensitization</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, Nov 1, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The sphingolipid ceramide modulates stress-induced cell death and apoptosis. We have shown that c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The sphingolipid ceramide modulates stress-induced cell death and apoptosis. We have shown that ceramide generated via de novo sphingolipid biosynthesis is required to initiate apoptosis after photodynamic therapy (PDT). The objective of this study was to define the role of ceramide synthase (CERS) in PDT-induced cell death and apoptosis using fumonisin B1 (FB), a CERS inhibitor. We used the silicon phthalocyanine Pc4 for PDT, and SCC17B cells, as a clinically-relevant model of human head and neck squamous carcinoma. zVAD-fmk, a pan-caspase inhibitor, as well as FB, protected cells from death after PDT. In contrast, ABT199, an inhibitor of the anti-apoptotic protein Bcl2, enhanced cell killing after PDT. PDT-induced accumulation of ceramide in the endoplasmic reticulum and mitochondria was inhibited by FB. PDT-induced Bax translocation to the mitochondria and cytochrome c release were also inhibited by FB. These novel data suggest that PDT-induced cell death via apoptosis is CERS/ceramide-dependent. † Electronic supplementary information (ESI) available. See</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="49e2c09590d5c5267fb2105ccabc8ea3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223491,"asset_id":107598977,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223491/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598977"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598977"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598977; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598976"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598976/Interaction_of_acid_ceramidase_inhibitor_LCL521_with_tumor_response_to_photodynamic_therapy_and_photodynamic_therapy_generated_vaccine"><img alt="Research paper thumbnail of Interaction of acid ceramidase inhibitor LCL521 with tumor response to photodynamic therapy and photodynamic therapy-generated vaccine" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598976/Interaction_of_acid_ceramidase_inhibitor_LCL521_with_tumor_response_to_photodynamic_therapy_and_photodynamic_therapy_generated_vaccine">Interaction of acid ceramidase inhibitor LCL521 with tumor response to photodynamic therapy and photodynamic therapy-generated vaccine</a></div><div class="wp-workCard_item"><span>International Journal of Cancer</span><span>, May 18, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Acid ceramidase has been identified as a promising target for cancer therapy. One of its most eff...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Acid ceramidase has been identified as a promising target for cancer therapy. One of its most effective inhibitors, LCL521, was examined as adjuvant to photodynamic therapy (PDT) using mouse squamous cell carcinoma SCCVII model of head and neck cancer. Lethal effects of PDT, assessed by colony forming ability of in vitro treated SCCVII cells, were greatly enhanced when combined with 10 µM LCL521 treatment particularly when preceding PDT. When PDT-treated SCCVII cells are used to vaccinate SCCVII tumor-bearing mice (PDT vaccine protocol), adjuvant LCL521 treatment (75 mg/kg) resulted in a marked retardation of tumor growth. This effect can be attributed to the capacity of LCL521 to effectively restrict the activity of two main immunoregulatory cell populations (Tregs and myeloid-derived suppressor cells, MDSCs) that are known to hinder the efficacy of PDT vaccines. The therapeutic benefit with adjuvant LCL521 was also achieved with SCCVII tumors treated with standard PDT when using immunocompetent mice but not with immunodeficient hosts. The interaction of LCL521 with PDT-based antitumor mechanisms is dominated by immune system contribution that includes overriding the effects of immunoregulatory cells, but could also include a tacit contribution from boosting direct tumor cell kill. Acid ceramidase, one of the key enzymes in sphingolipid metabolism, has been identified as a promising target for cancer therapy. Using a head and neck cancer mouse model, here the authors examine the effectiveness of acid ceramidase inhibitor LCL521 as adjuvant to photodynamic therapy (PDT). They show that targeting acid ceramidase can be successfully exploited in combined treatments with PDT or PDT-generated vaccines. LCL521 treatment enhances direct lethal effects initiated by PDT-induced oxidative stress responses in vitro. In vivo, the hindering effects of major immunoregulatory populations following PDT of tumors or related vaccine protocols can be restrained by LCL521 treatment. This article is protected by copyright. All rights reserved.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598976"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598976"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598976; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598976]").text(description); $(".js-view-count[data-work-id=107598976]").attr('title', description).tooltip(); 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</script> <div class="js-work-strip profile--work_container" data-work-id="107598975"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598975/Controlling_Immunoregulatory_Cell_Activity_for_Effective_Photodynamic_Therapy_of_Cancer"><img alt="Research paper thumbnail of Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598975/Controlling_Immunoregulatory_Cell_Activity_for_Effective_Photodynamic_Therapy_of_Cancer">Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer</a></div><div class="wp-workCard_item"><span>Springer eBooks</span><span>, 2022</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598975"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598975"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598975; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598975]").text(description); $(".js-view-count[data-work-id=107598975]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598975; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598975']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598975]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598975,"title":"Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer","internal_url":"https://www.academia.edu/107598975/Controlling_Immunoregulatory_Cell_Activity_for_Effective_Photodynamic_Therapy_of_Cancer","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598974"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598974/C6_pyridinium_ceramide_sensitizes_SCC17B_human_head_and_neck_squamous_cell_carcinoma_cells_to_photodynamic_therapy"><img alt="Research paper thumbnail of C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy" class="work-thumbnail" src="https://attachments.academia-assets.com/106223488/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598974/C6_pyridinium_ceramide_sensitizes_SCC17B_human_head_and_neck_squamous_cell_carcinoma_cells_to_photodynamic_therapy">C6-pyridinium ceramide sensitizes SCC17B human head and neck squamous cell carcinoma cells to photodynamic therapy</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Feb 1, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Combining photodynamic therapy (PDT) 1 with another anticancer treatment modality is an important...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Combining photodynamic therapy (PDT) 1 with another anticancer treatment modality is an important strategy for improved efficacy. PDT with Pc4, a silicon phthalocyanine photosensitizer, was combined with C6-pyridinium ceramide (LCL29) to determine their potential to promote death of SCC17B human head and neck squamous cell carcinoma cells. PDT+LCL29-induced enhanced cell death was inhibited by zVAD-fmk, a pan-caspase inhibitor, and fumonisin B1 (FB), a ceramide synthase inhibitor. Quantitative confocal microscopy showed that combining PDT with LCL29 enhanced FB-sensitive ceramide accumulation in the mitochondria. Furthermore, PDT +LCL29 induced enhanced FB-sensitive redistribution of cytochrome c and caspase-3 activation. Overall, the data indicate that PDT+LCL29 enhanced cell death via FB-sensitive, mitochondrial ceramide accumulation and apoptosis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f16c6e6cb10591d84969252f81e15761" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223488,"asset_id":107598974,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223488/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598974"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598974"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598974; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598973"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598973/Increased_tumour_dihydroceramide_production_after_Photofrin_PDT_alone_and_improved_tumour_response_after_the_combination_with_the_ceramide_analogue_LCL29_Evidence_from_mouse_squamous_cell_carcinomas"><img alt="Research paper thumbnail of Increased tumour dihydroceramide production after Photofrin-PDT alone and improved tumour response after the combination with the ceramide analogue LCL29. Evidence from mouse squamous cell carcinomas" class="work-thumbnail" src="https://attachments.academia-assets.com/106223487/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598973/Increased_tumour_dihydroceramide_production_after_Photofrin_PDT_alone_and_improved_tumour_response_after_the_combination_with_the_ceramide_analogue_LCL29_Evidence_from_mouse_squamous_cell_carcinomas">Increased tumour dihydroceramide production after Photofrin-PDT alone and improved tumour response after the combination with the ceramide analogue LCL29. Evidence from mouse squamous cell carcinomas</a></div><div class="wp-workCard_item"><span>British Journal of Cancer</span><span>, Feb 1, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Photodynamic therapy (PDT) has been proven effective for treatment of several types of cancer. Ph...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Photodynamic therapy (PDT) has been proven effective for treatment of several types of cancer. Photodynamic therapy alone, however, attains limited cures with some tumours and there is need for its improved efficacy in such cases. Sphingolipid (SL) analogues can promote tumour response in combination with anticancer drugs. In this study, we used mouse SCCVII squamous cell carcinoma tumours to determine the impact of Photofrin-PDT on the in vivo SL profile and the effect of LCL29, a C6-pyridinium ceramide, on PDT tumour response. Following PDT, the levels of dihydroceramides (DHceramides), in particular C20-DHceramide, were elevated in tumours. Similarly, increases in DHceramides, in addition to C20:1-ceramide, were found in PDT-treated SCCVII cells. These findings indicate the importance of the de novo ceramide pathway in Photofrin-PDT response not only in cells but also in vivo. Notably, co-exposure of SCCVII tumours to Photofrin-PDT and LCL29 led to enhanced tumour response compared with PDT alone. Thus, we show for the first time that Photofrin-PDT has a distinct signature effect on the SL profile in vitro and in vivo, and that the combined treatment advances PDT therapeutic gain, implying translational significance of the combination.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="4f9abcf283dceab3982f9fd20437ea12" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223487,"asset_id":107598973,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223487/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598973"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598973"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598973; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598973]").text(description); $(".js-view-count[data-work-id=107598973]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598973; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598973']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "4f9abcf283dceab3982f9fd20437ea12" } } $('.js-work-strip[data-work-id=107598973]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598973,"title":"Increased tumour dihydroceramide production after Photofrin-PDT alone and improved tumour response after the combination with the ceramide analogue LCL29. 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Elevated tumor C3 levels were detected between 1 and 24 h after PDT, while serum C3 levels increased significantly at 24 h post therapy. Increased alternative complement pathway activity in the serum was evident between 1 and 3 days post PDT. Blocking C3a-or C5a-receptors in the host mice decreased the efficacy of PDT in producing LLC tumor cures, supporting the importance of complement action in PDT-mediated tumor destruction.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="860be353d06cc0ec9bcfc81166cb54a4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223511,"asset_id":107598972,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223511/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598972"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598972"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598972; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598972]").text(description); $(".js-view-count[data-work-id=107598972]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598972; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598972']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "860be353d06cc0ec9bcfc81166cb54a4" } } $('.js-work-strip[data-work-id=107598972]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598972,"title":"Characteristics of complement activation in mice bearing Lewis lung carcinomas treated by photodynamic therapy","internal_url":"https://www.academia.edu/107598972/Characteristics_of_complement_activation_in_mice_bearing_Lewis_lung_carcinomas_treated_by_photodynamic_therapy","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[{"id":106223511,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223511/thumbnails/1.jpg","file_name":"characteristics-complement-activation-mice-lewis-lung-carcinomas-pdt.pdf","download_url":"https://www.academia.edu/attachments/106223511/download_file","bulk_download_file_name":"Characteristics_of_complement_activation.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223511/characteristics-complement-activation-mice-lewis-lung-carcinomas-pdt-libre.pdf?1696453825=\u0026response-content-disposition=attachment%3B+filename%3DCharacteristics_of_complement_activation.pdf\u0026Expires=1740033853\u0026Signature=OKCjnIj5VHUCESVaUYW2LfeGf3L8MVL3Lire0hh8Mfb-F7USi-Pp9uj5elNhq3E7~wma9afvBlijQLCYblPalgCMOzLkj4eA-0-GCbYcY2CLRokoiYj7pvJGaOSCUbJEkswdUY3FGlGrTNqvr~OV128e1pIfzwVVLUs3EzheEifd2LCZSVeoge7wECNtXuxbHurPjMsb7N1Z8MS34K3GwJUGKozbPrEmMkLSDN649LZ8vrHul8hhPV0yO67e5tV6ZMXzMjnGkOKZRWExTWWGf4CzfPR0my0~IEBFTwRkCkz7iu-Ym6SEXh-asDToZJXriDm91oyDLZ3IVKH8QNtm9w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598971"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598971/Fumonisin_B1_Inhibits_Endoplasmic_Reticulum_Stress_Associated_apoptosis_After_FoscanPDT_Combined_with_C6_Pyridinium_Ceramide_or_Fenretinide"><img alt="Research paper thumbnail of Fumonisin B1 Inhibits Endoplasmic Reticulum Stress Associated-apoptosis After FoscanPDT Combined with C6-Pyridinium Ceramide or Fenretinide" class="work-thumbnail" src="https://attachments.academia-assets.com/106223485/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598971/Fumonisin_B1_Inhibits_Endoplasmic_Reticulum_Stress_Associated_apoptosis_After_FoscanPDT_Combined_with_C6_Pyridinium_Ceramide_or_Fenretinide">Fumonisin B1 Inhibits Endoplasmic Reticulum Stress Associated-apoptosis After FoscanPDT Combined with C6-Pyridinium Ceramide or Fenretinide</a></div><div class="wp-workCard_item"><span>Anticancer Research</span><span>, Feb 10, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Background: Colorectal cancer is the third leading cause of cancer-related mortality in most deve...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Background: Colorectal cancer is the third leading cause of cancer-related mortality in most developed countries. This mortality is mainly due to the metastatic progression to the liver with frequent recurrence. Colorectal cancer remains a therapeutic challenge and this has intensified the search for new drug targets. In an effort to establish a novel targeted-therapy, we studied the molecular mechanisms of cancer stem cell inhibitor salinomycin. Materials and Methods: Co-immunoprecipitation was performed to examine STAT3-STAT1 protein interactions. Telomerase activity was measured by polymerase chain reaction (PCR) and ELISA assays. Apoptosis and cell stress arrays were analyzed to identify key proteins responding to salinomycin treatments. Results: IL-6 and TNF-α induced STAT3 and STAT1 interactions, however the interactions were abolished by salinomycin challenge. Salinomycin reduced cancer stem cell phenotype and decreased telomerase activity of colorectal cancer cells. Conclusion: Our work uncovers a new mechanism through which salinomycin inhibits cancer stemness suggesting a novel targeted-therapy for metastatic colorectal cancer.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7f0157ded8d4b8a33e3884776d993910" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223485,"asset_id":107598971,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223485/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598971"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598971"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598971; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598971]").text(description); $(".js-view-count[data-work-id=107598971]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598971; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598971']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598970"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598970/Enhanced_apoptotic_cancer_cell_killing_after_Foscan_photodynamic_therapy_combined_with_fenretinide_via_de_novo_sphingolipid_biosynthesis_pathway"><img alt="Research paper thumbnail of Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway" class="work-thumbnail" src="https://attachments.academia-assets.com/106223490/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598970/Enhanced_apoptotic_cancer_cell_killing_after_Foscan_photodynamic_therapy_combined_with_fenretinide_via_de_novo_sphingolipid_biosynthesis_pathway">Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Jun 1, 2016</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We and others have shown that stresses, including photodynamic therapy (PDT) 1 , can disrupt the ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We and others have shown that stresses, including photodynamic therapy (PDT) 1 , can disrupt the de novo sphingolipid biosynthesis pathway, leading to changes in the levels of sphingolipids, and subsequently, modulation of cell death. The de novo sphingolipid biosynthesis pathway includes a ceramide synthase-dependent reaction, giving rise to dihydroceramide, which is then converted in a desaturase-dependent reaction to ceramide. In this study we tested the hypothesis that combining Foscan-mediated PDT with desaturase inhibitor fenretinide (HPR) enhances cancer cell killing. We discovered that by subjecting SCC19 cells, a human head and neck squamous cell carcinoma cell line, to PDT+HPR resulted in enhanced accumulation of C16-dihydroceramide, not ceramide. Concomitantly, mitochondrial depolarization was enhanced by the combined treatment. Enhanced activation of caspase-3 after PDT +HPR was inhibited by FB. Enhanced clonogenic cell death after the combination was sensitive to FB, as well as Bcl2-and caspase inhibitors. Treatment of mouse SCCVII squamous cell carcinoma tumors with PDT+HPR resulted in improved long-term tumor cures. Overall, our data showed that combining PDT with HPR enhanced apoptotic cancer cell killing and antitumor efficacy of PDT. The data suggest the involvement of the de novo sphingolipid biosynthesis pathway in enhanced apoptotic cell killing after PDT+HPR, identify PDT+HPR as a more effective combination than either treatment alone, and that the combination has potential for cancer treatment.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="f0a1c466b649c3b68d26756b67f30e50" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223490,"asset_id":107598970,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223490/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598970"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598970"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598970; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598970]").text(description); $(".js-view-count[data-work-id=107598970]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598970; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598970']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "f0a1c466b649c3b68d26756b67f30e50" } } $('.js-work-strip[data-work-id=107598970]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598970,"title":"Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway","internal_url":"https://www.academia.edu/107598970/Enhanced_apoptotic_cancer_cell_killing_after_Foscan_photodynamic_therapy_combined_with_fenretinide_via_de_novo_sphingolipid_biosynthesis_pathway","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[{"id":106223490,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106223490/thumbnails/1.jpg","file_name":"pmc6123833.pdf","download_url":"https://www.academia.edu/attachments/106223490/download_file","bulk_download_file_name":"Enhanced_apoptotic_cancer_cell_killing_a.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106223490/pmc6123833-libre.pdf?1696453835=\u0026response-content-disposition=attachment%3B+filename%3DEnhanced_apoptotic_cancer_cell_killing_a.pdf\u0026Expires=1740033853\u0026Signature=Djhay54kTEsUbhV-CLxKILN8iiWcJFHUGPjJVgwP5WzkhNsrjMUrYovZcQm-BO0j~dD2ZSWAt1rkW1oLLFxiMmlEtd0JD2KggBFAcBNV7lJrYWhfLdvNxYYDiVHedyomSe77QurNLPaMf~hZH7OAFGsTh25qm2h8aysAGct4rRileoUyaVEeZsTmloze4mR4uDVkJ30ABznXSqBj9YTlkojhf-nBDlW5uMWdigCiHwDe1Qq6GYzx-hir2FWQ~qAQSU5puXTdwFuBdke0HcGj3Lvq18ApCdiSZk87aV7YIE~Qh8Qz6KeZYbx7do4Nl9kmQIpZLbop1s1HopongXNx2w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=107598969]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107598969,"title":"CA: A Cancer Journal for Clinicians","internal_url":"https://www.academia.edu/107598969/CA_A_Cancer_Journal_for_Clinicians","owner_id":33486112,"coauthors_can_edit":true,"owner":{"id":33486112,"first_name":"Mladen","middle_initials":null,"last_name":"Korbelik","page_name":"MladenKorbelik","domain_name":"ubc","created_at":"2015-07-31T04:32:57.787-07:00","display_name":"Mladen Korbelik","url":"https://ubc.academia.edu/MladenKorbelik"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598968"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/107598968/Ceramide_and_Sphingosine_1_Phosphate_Sphingosine_act_as_Photodynamic_Therapy_Elicited_Damage_Associated_Molecular_Patterns_Release_from_Cells_and_Impact_on_Tumor_Associated_Macrophages"><img alt="Research paper thumbnail of Ceramide and Sphingosine-1-Phosphate/Sphingosine act as Photodynamic Therapy-Elicited Damage-Associated Molecular Patterns: Release from Cells and Impact on Tumor-Associated Macrophages" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/107598968/Ceramide_and_Sphingosine_1_Phosphate_Sphingosine_act_as_Photodynamic_Therapy_Elicited_Damage_Associated_Molecular_Patterns_Release_from_Cells_and_Impact_on_Tumor_Associated_Macrophages">Ceramide and Sphingosine-1-Phosphate/Sphingosine act as Photodynamic Therapy-Elicited Damage-Associated Molecular Patterns: Release from Cells and Impact on Tumor-Associated Macrophages</a></div><div class="wp-workCard_item"><span>Journal of analytical & bioanalytical techniques</span><span>, 2014</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598968"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598968"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598968; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107598968]").text(description); $(".js-view-count[data-work-id=107598968]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107598968; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107598968']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598967"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598967/Mechanistic_insights_into_ceramidase_inhibitor_LCL521_enhanced_tumor_cell_killing_by_photodynamic_and_thermal_ablation_therapies"><img alt="Research paper thumbnail of Mechanistic insights into ceramidase inhibitor LCL521-enhanced tumor cell killing by photodynamic and thermal ablation therapies" class="work-thumbnail" src="https://attachments.academia-assets.com/106223507/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598967/Mechanistic_insights_into_ceramidase_inhibitor_LCL521_enhanced_tumor_cell_killing_by_photodynamic_and_thermal_ablation_therapies">Mechanistic insights into ceramidase inhibitor LCL521-enhanced tumor cell killing by photodynamic and thermal ablation therapies</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, Sep 1, 2020</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Our recent investigation uncovered that the acid ceramidase inhibitor LCL521 enhances the direct ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Our recent investigation uncovered that the acid ceramidase inhibitor LCL521 enhances the direct tumor cell killing effect of photodynamic therapy (PDT) treatment. The present study aimed at elucidating the mechanisms underlying this effect. Exposing mouse squamous cell carcinoma SCCVII cells treated with temoporfin-based PDT to LCL521 (rising ceramide concentration) produced a much greater decrease in cell survival than comparable exposure to the sphingosine kinase-1 inhibitor PF543 (that reduces sphingosine-1-phosphate concentration). This is consistent with recognizing the rising levels of pro-apoptotic sphingolipid ceramide as being more critical in promoting the death of PDT-treated cells than the reduction in the availability of pro-survival acting sphingosine-1 phosphate. This pro-apoptotic impact of LCL521, which was suppressed by the apoptosis inhibitor bongkrekic acid, involves the interaction with the cellular stress signaling network. Hence, inhibiting the key elements of these pathways markedly influenced the adjuvant effect of LCL521 on the PDT response. Particularly effective was the inositolrequiring element-1 (IRE1) kinase inhibitor STF-083010 that dramatically enhanced the killing of cells treated with PDT plus LCL521. An important role in the survival of these cells was exhibited by master transcription factors STAT3 and HIF-1α. The STAT3 inhibitor NSC 74859 was especially effective in further reducing the cell survival rates, suggesting its possible exploitation for therapeutic gain. An additional finding in this study is that LCL521-promoted PDT-mediated cell killing through ceramide-mediated lethal effects is extended to the interaction with other cancer treatment modalities with a rapid cellular stress impact such as photothermal therapy (PTT) and cryoablation therapy (CAT).</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="917b7ef79340a824f7d87a3c66456346" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223507,"asset_id":107598967,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223507/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598967"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598967"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598967; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598966"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598966/Drug_delivery_technologies_and_immunological_aspects_of_photodynamic_therapy"><img alt="Research paper thumbnail of Drug delivery technologies and immunological aspects of photodynamic therapy" class="work-thumbnail" src="https://attachments.academia-assets.com/106223505/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598966/Drug_delivery_technologies_and_immunological_aspects_of_photodynamic_therapy">Drug delivery technologies and immunological aspects of photodynamic therapy</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, May 1, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="619b43fbe37bdeec712277bac4700fba" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223505,"asset_id":107598966,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223505/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598966"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598966"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598966; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598965"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598965/Enhancement_of_tumour_response_to_photodynamic_therapy_by_adjuvant_mycobacterium_cell_wall_treatment"><img alt="Research paper thumbnail of Enhancement of tumour response to photodynamic therapy by adjuvant mycobacterium cell-wall treatment" class="work-thumbnail" src="https://attachments.academia-assets.com/106223508/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598965/Enhancement_of_tumour_response_to_photodynamic_therapy_by_adjuvant_mycobacterium_cell_wall_treatment">Enhancement of tumour response to photodynamic therapy by adjuvant mycobacterium cell-wall treatment</a></div><div class="wp-workCard_item"><span>Journal of Photochemistry and Photobiology B-biology</span><span>, Jul 1, 1998</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Mycobacterium cell-wall extract (MCWE) is a potent non-specific immunostimulant that elicits a lo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Mycobacterium cell-wall extract (MCWE) is a potent non-specific immunostimulant that elicits a local inflammatory response associated with antitumour activity. Tumour-localized administration of MCWE has been examined as an adjuvant to photodynamic therapy (PDT) mediated by the photosensitizers Photofrin, benzoporphyrin derivative monoacid (BPD), metatetrahydroxyphenylchlorin (mTHPC), or zinc (II)-phthaIocyanine (ZnPc). A single MCWE treatment, given immediately after light treatment of murine EMT6 tumours, potentiates the curative effect of PDT. A similar enhancement of tumour response to Photofrin-based PDT is obtained with the live Bacillus Calmerte-Guhrin (BCG) vaccine. Despite differences in the kinetics/intensity of damage induction to tumour microvasculature and othercharacteristics underlying the mechanism of antitumour activity of Photofrin, BPD, mTHPC and ZnPc, there appear to be no marked differences in the therapeutic benefit of adjuvant MCWE therapy combined with the PDT mediated by these various photosensitizers. This may be related to the fact that MCWE elicits a wide range of immunomodulatory effects that could amplify and sustain the inflammatory/immune responses triggered by PDT. The enhancement of inflammatory effector cell activity is indicated by the increased infiltration of neutrophils and other myeloid cells at the expense of malignant cells found in the MCWE plus mTHPC-based PDT treatment group compared to the PDT-only group.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="860c4a2618c637410b403515817e9e61" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223508,"asset_id":107598965,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223508/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598965"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598965"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598965; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598964"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598964/Induction_of_Systemic_Neutrophil_Response_in_Mice_by_Photodynamic_Therapy_of_Solid_Tumors_"><img alt="Research paper thumbnail of Induction of Systemic Neutrophil Response in Mice by Photodynamic Therapy of Solid Tumors¶" class="work-thumbnail" src="https://attachments.academia-assets.com/106223516/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598964/Induction_of_Systemic_Neutrophil_Response_in_Mice_by_Photodynamic_Therapy_of_Solid_Tumors_">Induction of Systemic Neutrophil Response in Mice by Photodynamic Therapy of Solid Tumors¶</a></div><div class="wp-workCard_item"><span>Photochemistry and Photobiology</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Photodynamic therapy (PDT) of solid tumors elicits a strong, acute inflammatory response characte...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Photodynamic therapy (PDT) of solid tumors elicits a strong, acute inflammatory response characterized by a rapid and massive infiltration of activated neutrophils into the tumor. The present study investigated the impact of PDT on the systemic and local (treatment site) kinetics of neutrophil trafficking and activity in mouse SCCVII and EMT6 tumor models. Differential leukocyte counts in the peripheral blood of treated mice revealed a pronounced neutrophilia developing rapidly after Photofrin porfimer sodium (Photofrin)-or tetra(m-tetrahydroxyphenyl)chlorin (mTHPC)-based PDT. Significant neutrophilia was also observed upon PDT treatment of normal dorsal skin but not on the footpad of tumor-free mice. The changes in circulating neutrophil numbers were accompanied by an efflux of these cells from the bone marrow. An increased proportion of cells with high L-selectin (CD62L antigen) expression was found among bone-marrow-residing neutrophils 6-24 h after PDT, and in neutrophils in the peripheral circulation and treated tumors 24 h after therapy. Complement inhibition completely prevented the development of PDT-induced neutrophilia. The results of the present study demonstrate that treatment of solid tumors by PDT induces a strong and protracted increase in systemic neutrophil numbers mediated by complement activation. This reaction reflects rapid and massive mobilization and activation of neutrophils for the destruction of PDT-treated tumor tissue.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="83cfe4f7f58e2489ca03e9fd71567ff5" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223516,"asset_id":107598964,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223516/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598964"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598964"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598964; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107598963"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107598963/Neutrophils_as_inflammatory_and_immune_effectors_in_photodynamic_therapy_treated_mouse_SCCVII_tumours"><img alt="Research paper thumbnail of Neutrophils as inflammatory and immune effectors in photodynamic therapy-treated mouse SCCVII tumours" class="work-thumbnail" src="https://attachments.academia-assets.com/106223506/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107598963/Neutrophils_as_inflammatory_and_immune_effectors_in_photodynamic_therapy_treated_mouse_SCCVII_tumours">Neutrophils as inflammatory and immune effectors in photodynamic therapy-treated mouse SCCVII tumours</a></div><div class="wp-workCard_item"><span>Photochemical and Photobiological Sciences</span><span>, Jul 16, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Neutrophils have become recognised as important contributors to the effectiveness of tumour eradi...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Neutrophils have become recognised as important contributors to the effectiveness of tumour eradication by photodynamic therapy (PDT). In this study, we have used the mouse SCCVII squamous cell carcinoma model to investigate the activity of neutrophils in tumours treated by PDT. Tumour levels of neutrophilic myeloperoxidase (MPO) demonstrated not only a massive and sustained sequestration of these cells in PDT-treated tumours but also revealed their activated state evidenced by the presence of released MPO. Among the adhesion molecules expressed on tumour vascular endothelium, ICAM-1 appears to be of primary importance in the invasion of neutrophils into PDT-treated tumours, because its functional blocking with monoclonal antibodies reduced the tumour cure rate. A marked upregulation of its ligands CD11b/CD18 and CD11c/CD18 found on neutrophils associated with PDTtreated tumours supports this assumption. To evaluate the role of inflammatory cytokines regulating neutrophil activity, neutralising antibodies were given to mice before PDT treatment. The results suggest that IL-1β activity is critical for the therapeutic outcome, since its neutralisation diminished the cure rates of PDT-treated tumours. No significant effect was observed with anti-IL-6 and anti-TNF-α treatment. Further flow cytometry-based examination of neutrophils found in PDT-treated tumours revealed that these cells express MHC class II molecules, which suggests their engagement as antigen-presenting cells and involvement in the development of antitumour immune response.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="611484edb3c5d985e42b26bf696d6268" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106223506,"asset_id":107598963,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106223506/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107598963"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107598963"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107598963; 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