Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay (FCSTM14) by R&D Systems, Part of Bio-Techne

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B Custom LxPA Mag Kit, Fluorokine MAP Multiplex, FMAP, Bio-Plex, Magentic Beads" /> <meta name="description" content="Build your own Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay with our Luminex Assay Customization Tool. Our Luminex multiplexing immunoassays include cytokines and chemokines and are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. 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Earn double rewards for reviews </div> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> <div id="product_alerts_wrapper"><article class="product-data-wrapper"> </article> </div> </div> <div class="row border-top" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.bio-techne.com/images/products/fcstm14_human-hs-cytokine-b-premixed-mag-luminex-performance-assay-1811202013265.png" alt="Human Cytokine B High Sensitivity Luminex Panel Standard Curves" title="Human Cytokine B High Sensitivity Luminex Panel Standard Curves" data-caption="" data-badges="" data-application="Multiplex Immunoassay" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/fcstm14_human-hs-cytokine-b-premixed-mag-luminex-performance-assay-2872020162950.jpg" data-alt="Human High Sensitivity Cytokine B Premixed Magnetic Luminex Performance Assay" title="Human High Sensitivity Cytokine B Premixed Magnetic Luminex Performance Assay" data-caption="" data-badges="" data-application="Multiplex Immunoassay" data-width="250" data-height="250" class="none"></i> </div> </div> </article> </div> <div class="col-xl-8" id="atc_wrapper"><article class="product-data-wrapper"> <h3>Luminex High Performance Assays</h3> <p>Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.</p> <p>Build your own Luminex Assay with our Luminex Assay Customization Tool.</p> <hr class="a___border-dashed my-4"/> <p> <a class="btn btn-primary text-white float-right mb-2" id="luminex_build_now_btn" href="#luminex_analyte_page"> Build Now </a> </p> </article> </div> </div> <div class="row datapoint_list border-top" id="key_details_wrapper"><div class="col-xl-12 mt-2" id="key_details_col_wrapper"></div> </div> </div> <div class="col-xl-3 mt-2" id="orderinfo_guarantee_wrapper"><div id="order_information_wrapper"><div id="product_shipping_block_wrapper" data-brand="rnd" data-shipping="" data-source="ep-data" data-sub_category_id="80"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"><span class="sr-only">Loading...</span></div> </div> </div> <div id="video-tile-wrapper"><article class="product-data-wrapper"> <div class="video_tile_wrapper"> <article> <div> <lite-youtube videoid="9QE6w3gImsM" style="background-image: url('https://img.youtube.com/vi/9QE6w3gImsM/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/9QE6w3gImsM?autoplay=1" class="lty-playbtn" title="Setting the standard in quality research reagents"> <span class="lyt-visually-hidden">Setting the standard in quality research reagents</span> </a> </lite-youtube> </div> </article> </div> </article> </div> </div> </div> <div class="datapoint_list table tablesaw tablesaw-stack" id="key_details"><h3>Key Product Details</h3><table class=" table" id="key_details_table_wrapper"><tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Panel: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Luminex Performance Human High Sensitivity Cytokine Panel B </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Assay Type: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Magnetic bead-based multiplex assay for the Luminex® MAGPIX® platform </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Format: </td> <td class="product-data-value py-2 align-top w-75 text-break"> 96-well microplate and antibody-coated magnetic beads* </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Species: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Human </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Analytes Detected: </td> <td class="product-data-value py-2 align-top w-75 text-break"> <div class="luminex-panel"> <ul class="luminex-analytes p-0"> <li>GM-CSF</li> <li>IFN-gamma</li> <li>IL-1 beta/IL-1F2</li> <li>IL-2</li> <li>IL-5</li> <li>IL-6</li> <li>IL-7</li> <li>IL-13</li> <li>IL-15</li> <li>IL-17/IL-17A</li> <li>IL-17F</li> <li>IL-22</li> <li>IL-23</li> <li>IL-31</li> <li>IL-33</li> <li>IL-36 beta/IL-1F8</li> <li>TNF-alpha</li> </ul> </div> </td> </tr> </table> <p>Not finding your analyte of interest? View our complete Luminex offering in the full version of our <a href="/luminex-assay-customization-tool">Luminex Assay Customization Tool</a>. We also offer <a href="https://www.rndsystems.com/services/luminex-assays-custom-development-services" target="_blank">Custom Luminex Services <span class="icon-launch"></span></a>. </p> </div> <div id="view_all_category_link_wrapper"><div class="text-left pl-3"> <h4><a href="/t/multiplex-cytokine-assay-kits/luminex-assays">View all Multiplex Cytokine Assay Kits Luminex Assays »</a></h4> </div></div> <div id="view_technical_detail_links"><div class="text-center"> <a id="view_technical" href="#technical-data-tab-tab">View Full Technical Details for Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay</a> </div> </div> </div> <div class="tab-content"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" aria-controls="region-6521588722589" aria-selected="true" >Technical Data</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-reviews--tab-tab" href="#citations-reviews--tab" data-toggle="tab" role="tab" aria-controls="region-2961587486424" aria-selected="false" >Citations / Reviews (2)</a> </li> </ul> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4" id="technical-data-tab" data-tab-label="Technical Data" role="tabpanel" aria-labelledby="technical-data-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="product-features_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#product-features" id="product-features_li" >Product Features</a> </li> <li id="performance-validation_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#performance-validation" id="performance-validation_li" >Performance Validation</a> </li> <li id="kit-components_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#kit-components" id="kit-components_li" >Kit Components</a> </li> <li id="precision-and-accuracy_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#precision-and-accuracy" id="precision-and-accuracy_li" >Precision and Accuracy</a> </li> <li id="build-your-assay_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#build-your-assay" id="build-your-assay_li" >Build Your Assay</a> </li> <li id="which-luminex-assay-is-right-for-me_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#which-luminex-assay-is-right-for-me" id="which-luminex-assay-is-right-for-me_li" >Which Luminex Assay is right for me?</a> </li> <li id="about-magnetic-luminex-assays_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#about-magnetic-luminex-assays" id="about-magnetic-luminex-assays_li" >About Magnetic Luminex Assays</a> </li> <li id="scientific-data_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#scientific-data" id="scientific-data_li" >Scientific Data</a> </li> <li id="preparation-and-storage_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#preparation-and-storage" id="preparation-and-storage_li" >Preparation and Storage</a> </li> <li id="product-specific-notices_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#product-specific-notices" id="product-specific-notices_li" >Product Specific Notices</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-features" class="nav-link px-0" data-anchor-label="Product Features"><div id="luminex_hp_product_features_wrapper"><h3>Product Features</h3><p>Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.</p> <ul> <li>Simultaneously profile up to 17 analytes of your choice in one sample</li> <li>Magnetic format allows for easier wash steps</li> <li>Undergoes similar validation testing as Quantikine® ELISA Kits</li> <li>Precise and Specific</li> <li>Requires a small sample volume (<50 µL)</li> <li>Run your assay in just 3-5 hours</li> <li>Mass-calibrated standards for consistent results with every new lot of material</li> <li>Enough reagents for one 96-well plate</li> </ul> </div> </div> <div id="performance-validation" class="nav-link px-0" data-anchor-label="Performance Validation"><div id="luminex_hp_validation"><h3>Performance Validation</h3><p>Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet. </p> </div> </div> <div id="kit-components" class="nav-link px-0" data-anchor-label="Kit Components"><h3>Kit Components</h3> <ul> <li>Premixed cocktail of antibody-coated Magnetic beads</li> <li>Premixed cocktail of biotinylated detection antibody</li> <li>Standard Cocktail(s)</li> <li>Bead Diluent</li> <li>Biotin Antibody Diluent</li> <li>Calibrator Diluent</li> <li>Wash Buffer</li> <li>Streptavidin-PE</li> <li>One flat-bottom 96-well Microplate</li> <li>Foil Plate Sealers (4)</li> <li>Mixing Bottle</li> <li>Standard Value Card**</li> </ul> <p> **A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay. </p> </div> <div id="precision-and-accuracy" class="nav-link px-0" data-anchor-label="Precision and Accuracy"><div id="precision_accuracy_wrapper"><article class="product-data-wrapper"> <div class="col-xs-12"> <div class=""> <h3>Precision</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td align="left" colspan="3"> <p>Intra-Assay Precision - is generated from the mean of the %CV’s from 20 reportable results across two different concentrations of analytes in a single serum assay.</p> <p>Inter-Assay Precision - is generated from the mean of the %CV’s across two different concentrations of analytes across 43 different serum assays.</p> </td> </tr> <tr> <th> </th> <th>n=20 (10x2 levels)</th> <th>n=43</th> </tr> <tr> <th>Analyte</th> <th>Intra-Assay (%CV)</th> <th>Inter-Assay (%CV)</th> </tr> </thead> <tbody> <tr> <td>GM-CSF</td> <td>1.7</td> <td>9.8</td> </tr> <tr> <td>IFN-gamma</td> <td>1.5</td> <td>15.6</td> </tr> <tr> <td>IL-1 beta</td> <td>1.7</td> <td>11.1</td> </tr> <tr> <td>IL-2</td> <td>2.0</td> <td>11.4</td> </tr> <tr> <td>IL-5</td> <td>3.2</td> <td>14.1</td> </tr> <tr> <td>IL-6</td> <td>2.0</td> <td>11.4</td> </tr> <tr> <td>IL-7</td> <td>2.4</td> <td>12.4</td> </tr> <tr> <td>IL-13</td> <td>2.9</td> <td>12.3</td> </tr> <tr> <td>IL-15</td> <td>2.0</td> <td>12.7</td> </tr> <tr> <td>IL-17A</td> <td>1.6</td> <td>11.7</td> </tr> <tr> <td>IL-17F</td> <td>2.5</td> <td>9.9</td> </tr> <tr> <td>IL-22</td> <td>2.4</td> <td>16.4</td> </tr> <tr> <td>IL-23</td> <td>1.1</td> <td>15.0</td> </tr> <tr> <td>IL-31</td> <td>2.3</td> <td>14.8</td> </tr> <tr> <td>IL-33</td> <td>1.9</td> <td>13.3</td> </tr> <tr> <td>IL-36 beta</td> <td>2.5</td> <td>13.0</td> </tr> <tr> <td>TNF-alpha</td> <td>1.7</td> <td>11.6</td> </tr> </tbody> </table> </div> <div class="col-xs-12"> <div class=""> <h3>Accuracy</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td colspan="5"> <p>Linearity - The data represents mean spiked or natural linearity in serum matrix samples.</p> <p>Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.</p> </td> </tr> <tr> <th> </th> <th align="center" colspan="2">Linearity</th> <th align="center" colspan="2">Spiked Recovery</th> </tr> <tr> <th>Analyte</th> <th>N</th> <th>Average %</th> <th>N</th> <th>Average %</th> </tr> </thead> <tfoot> </tfoot> <tbody> <tr> <td>GM-CSF</td> <td>4</td> <td>92</td> <td>4</td> <td>104</td> </tr> <tr> <td>IFN-gamma</td> <td>4</td> <td>96</td> <td>4</td> <td>101</td> </tr> <tr> <td>IL-1 beta</td> <td>4</td> <td>94</td> <td>4</td> <td>111</td> </tr> <tr> <td>IL-2</td> <td>4</td> <td>96</td> <td>4</td> <td>98</td> </tr> <tr> <td>IL-5</td> <td>4</td> <td>101</td> <td>4</td> <td>103</td> </tr> <tr> <td>IL-6</td> <td>4</td> <td>107</td> <td>4</td> <td>83</td> </tr> <tr> <td>IL-7</td> <td>4</td> <td>101</td> <td>4</td> <td>94</td> </tr> <tr> <td>IL-13</td> <td>4</td> <td>91</td> <td>4</td> <td>117</td> </tr> <tr> <td>IL-15</td> <td>4</td> <td>94</td> <td>4</td> <td>110</td> </tr> <tr> <td>IL-17A</td> <td>4</td> <td>112</td> <td>4</td> <td>100</td> </tr> <tr> <td>IL-17F</td> <td>4</td> <td>118</td> <td>4</td> <td>75</td> </tr> <tr> <td>IL-22</td> <td>4</td> <td>105</td> <td>4</td> <td>86</td> </tr> <tr> <td>IL-23</td> <td>4</td> <td>94</td> <td>4</td> <td>78</td> </tr> <tr> <td>IL-31</td> <td>4</td> <td>94</td> <td>4</td> <td>108</td> </tr> <tr> <td>IL-33</td> <td>4</td> <td>90</td> <td>4</td> <td>125</td> </tr> <tr> <td>IL-36 beta</td> <td>4</td> <td>101</td> <td>4</td> <td>105</td> </tr> <tr> <td>TNF-alpha</td> <td>4</td> <td>107</td> <td>4</td> <td>94</td> </tr> </tbody> </table> </div> </article> </div> </div> <div id="build-your-assay" class="nav-link px-0" data-anchor-label="Build Your Assay"><div id="luminex_tool_wrapper"><article class="product-data-wrapper"> <div id="luminex_analyte_page" class="page-container luminex-analyte-page"> <div class="row"> <div class="luminex-whole-column col-sm-12"> <h2 class="luminex-analytes-summary"> Build Your Assay </h2> <h3 class="luminex-analytes-summary"> Make Your Selections Below to Configure Your Custom Luminex Assay </h3> <div class="luminex-analytes-filter-container luminex-hide-on-load"> <div class="luminex-analytes-filter-element-wrapper"> <span class="luminex-analytes-filter-element luminex-analytes-filter-label">Filter Analytes</span> <span class="luminex-analytes-filter-element luminex-analytes-filter-input-area"> <input type="text" class="luminex-analytes-filter-input pr-4" id="luminex_input_filter_results" placeholder="Analyte"/> <span class="luminex-button luminex-button-filter-clear luminex-hide-on-load" id="luminex_button_filter_clear"></span> </span>  </div>  <div class="luminex-analytes-select-wrapper"> <span class="luminex-analytes-filter-element luminex-analytes-select-all-container luminex-button-wrapper"> <span class="btn btn-quinary luminex-button luminex-button-select-all" id="luminex_button_select_all">Select All</span> </span>  <span class="luminex-analytes-filter-element luminex-analytes-select-none-container luminex-button-wrapper"> <span class="btn btn-quinary luminex-button luminex-button-select-none" id="luminex_button_select_none">Select None</span> </span>  </div>  </div>  <div class="luminex-analytes-results row" id="luminex_analytes_results"> </div>  <div class="luminex-assays-section luminex-right-side luminex-hide-on-load"> <div class="row"> <div class="luminex-kits-header col-sm-12"> <h2 class="luminex-kits-summary">Assays</h2> <p class="luminex-kits-instructions"> Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed. </p> <p><b>If you run into bead region incompatibilities, <a href="https://www.rndsystems.com/services/luminex-assays-custom-development-services" target="_blank">send your Luminex code</a> to our team and find out more.</b></p> </div> </div> <div class="luminex-splitting-area row"> <div class="luminex-splitting-mixing luminex-splitting-box col-xs-12 col-sm-6" id="luminex_splitting_mixing"> <div class="luminex-splitting-row luminex-splitting-row-header"> <h4 class="luminex-mixing-question luminex-splitting-question">How would you like these analytes mixed?</h4> </div>  <div class="luminex-splitting-row luminex-splitting-row-premixed"> <div class="luminex-splitting-row-select-premixed luminex-splitting-row-select-mixing luminex-splitting-row-select luminex-mixing-selected" data-mixing="premixed">Premixed<span class="mixing-info-icon structure-info-icon info-icon luminex-info-icon-mixing luminex-info-icon-premixed" data-toggle="popover" data-placement="left" data-title="Beads and biotin antibodies are mixed before shipment" data-group="luminex-info-icon-mixing" data-name="luminex-info-icon-premixed"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-user-mixed"> <div class="luminex-splitting-row-select-user-mixed luminex-splitting-row-select-mixing luminex-splitting-row-select" data-mixing="user-mixed">User Mixed<span class="mixing-info-icon structure-info-icon info-icon luminex-info-icon-mixing luminex-info-icon-user-mixed" data-placement="left" data-title="Mix your own beads and biotin antibodies when your need them" data-group="luminex-info-icon-mixing" data-name="luminex-info-icon-user-mixed"></span></div> </div>  <div class="luminex-splitting-row-alert luminex-hide-on-load"> <div class="luminex-splitting-row-alert-content alert alert-warning">Please select at least one mixing option to see the kits.</div> </div>  </div>  <div class="luminex-splitting-dilution luminex-splitting-box col-xs-12 col-sm-6" id="luminex_splitting_dilution"> <div class="luminex-splitting-row luminex-splitting-row-header"> <h4 class="luminex-dilution-question luminex-splitting-question">Would you like to split based on dilution?</h4> </div>  <div class="luminex-splitting-row luminex-splitting-row-do-not-split"> <div class="luminex-splitting-row-select-do-not-split luminex-splitting-row-select-dilution luminex-splitting-row-select luminex-dilution-selected" data-dilution="do-not-split">Do not split<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-" data-placement="left" data-title="Different sample type dilutions are not considered" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-do-not-split"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-split-serum"> <div class="luminex-splitting-row-select-split-serum luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-serum">Split based on serum<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-serum" data-placement="left" data-title="Panel is split based upon matching serum sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-serum"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-split-plasma"> <div class="luminex-splitting-row-select-split-plasma luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-plasma">Split based on plasma<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-plasma" data-placement="left" data-title="Panel is split based upon matching plasma sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-plasma"></span></div> </div>  <div class="luminex-splitting-row luminex-splitting-row-split-cell-culture"> <div class="luminex-splitting-row-select-split-cell-culture luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-cell-culture">Split based on cell culture supernates<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-cell-culture" data-placement="left" data-title="Panel is split based upon matching cell culture supernate sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-cell-culture"></span></div> </div>  </div>  </div>  <div class="luminex-kits-area row" id="luminex_kits_area"> </div>  </div>  </div>  </div>  </div>  </article> </div> </div> <div id="which-luminex-assay-is-right-for-me" class="nav-link px-0" data-anchor-label="Which Luminex Assay is right for me?"><div id="luminex_wrapper"><h3>Which Luminex Assay is Right For Me?</h3></div> <p>Assays for the Luminex platform are offered as <a href="https://www.rndsystems.com/products/luminex-high-performance-assays?utm_source=bio-techne.com&utm_medium=referral&utm_campaign=sister-link&utm_term=FCSTM14&utm_content=luminex-product-page-link" target="_blank">High Performance Assays <span class="icon-launch"></span></a> or Discovery <a href="https://www.rndsystems.com/products/luminex-assays?utm_source=bio-techne.com&utm_medium=referral&utm_campaign=sister-link&utm_term=FCSTM14&utm_content=luminex-product-page-link" target="_blank">Assays <span class="icon-launch"></span></a>. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. <a href="https://www.rndsystems.com/products/which-luminex-assay-right-me?utm_source=bio-techne.com&utm_medium=referral&utm_campaign=sister-link&utm_term=FCSTM14&utm_content=luminex-product-page-link" target="_blank">View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com <span class="icon-launch"></span></a>.</p> </div> <div id="about-magnetic-luminex-assays" class="nav-link px-0" data-anchor-label="About Magnetic Luminex Assays"><div id="luminex_about_magnetic"><h3>More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform</h3></div> <p>Magnetic <a href="https://www.rndsystems.com/products/luminex-high-performance-assays?utm_source=bio-techne.com&utm_medium=referral&utm_campaign=sister-link&utm_term=FCSTM14&utm_content=luminex-product-page-link" target="_blank">Luminex High Performance Assays <span class="icon-launch"></span></a> are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the <a href="/p/luminex-instrument/luminex-200-ruo-system-w-xponent-42_lx200-xpon-ruo">Luminex 200™</a> or <a href="/p/luminex-instrument/flexmap-3d-with-xponent-42_flexmap-3d-ruo">FLEXMAP 3D</a>, dual laser, flow-based sorting and detection platforms.</p> <p>Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.</p> </div> <div id="scientific-data" class="nav-link px-0" data-anchor-label="Scientific Data"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>Scientific Data Examples</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/fcstm14_human-hs-cytokine-b-premixed-mag-luminex-performance-assay-1811202013265.png" data-srcset="https://resources.bio-techne.com/images/products/fcstm14_human-hs-cytokine-b-premixed-mag-luminex-performance-assay-1811202013265.png" alt="" title="Human Cytokine B High Sensitivity Luminex Panel Standard Curves" data-caption="" data-badges="" data-application="Multiplex Immunoassay" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Human Cytokine B High Sensitivity Luminex Panel Standard Curves</strong> </p> </td> </tr> </article> </table> </div> <div id="preparation-and-storage" class="nav-link px-0" data-anchor-label="Preparation and Storage"><div id="elisa_preparation_storage"><h3>Preparation and Storage</h3><table class="table-bordered table" id="elisa_preparation_storage_table"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Stability & Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at 2 - 8 °C. Do not use past expiration date. </td> </tr> </table> </div> </div> <div id="product-specific-notices" class="nav-link px-0" data-anchor-label="Product Specific Notices"><div id="product_specific_notices"><h3>Product Specific Notices</h3><article class="product-data-wrapper"> Luminex® is a registered trademark of Luminex Corporation. </article> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4" id="citations-reviews--tab" data-tab-label="Citations / Reviews (2)" role="tabpanel" aria-labelledby="citations-reviews--tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> </div> </div> <div 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<div class="row"> <div class="col-12"> <h2>Certificate of Analysis</h2> <p>To download a Certificate of Analysis, please enter a lot number in the search box below.</p> <p><strong>Note:</strong> Certificate of Analysis not available for kit components.</p> </div> </div> <section class="row"> <div class="col-md-6"> <form novalidate="novalidate" data-drupal-selector="product-coa-form"> <div class="form-row align-items-center"> <div class="col-md-10 my-1"> <input data-drupal-selector="form-2c4ginyk-6yd87alzy0-kur1jsvsmjiowb1pawvbd2y" type="hidden" name="form_build_id" value="form-2c4gINyK-6YD87alZY0_KuR1JSVsMJiOWB1PawVBD2Y" /> <input data-drupal-selector="edit-product-coa-form" type="hidden" name="form_id" value="product_coa_form" /> <input data-drupal-selector="edit-product-code" type="hidden" name="product_code" value="FCSTM14" /> <div class="js-form-item form-item pb-3 js-form-type-textfield form-item-lot-number js-form-item-lot-number form-no-label"> <input autocomplete="off" 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Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay (FCSTM14) by R&D Systems, Part of Bio-Techne