Next Generation Sequencing Sample Submission Guidelines - Resources

<!DOCTYPE html>    <html lang="en">  <head> <title>Next Generation Sequencing Sample Submission Guidelines - Resources - GENEWIZ</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content=""> <meta name="keywords" content=""> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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document, '//static.hotjar.com/c/hotjar-', '.js?sv='); </script>  <script src="https://cdn.cookielaw.org/scripttemplates/otSDKStub.js" type="text/javascript" charset="UTF-8" data-domain-script="0190a77c-46be-74e5-a65b-09fc07bbf439" ></script> <script type="text/javascript"> function OptanonWrapper() { } </script>   <p style="display:none;"><button id="ot-sdk-btn" class="ot-sdk-show-settings">CookieSettings</button></p>  <br /><br /> <div class="headline-center Rich-Text-Edit-Area"> <style> .guidelines-container { text-align: center; margin-top: 50px; } .guidelines-container h3 { color: #000; font-size: 20px; } .list-item { display: inline-block; padding: 20px; text-align: center; } .cognito #c-submit-button { display:none; } </style> <div> </div> <div> </div> <div id="backtoTop" class="container text-center"> <h1 style="text-align: center;">Next Generation Sequencing</h1> <p>View our Sample Submission <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://fs.hubspotusercontent00.net/hubfs/3478602/13007-M&G 1221V3.pdf" target="_blank'">Best Practices Checklist</a>.</p> <br /> <h3 style="text-align: center; font-size: 21px;">Interactive Sample Submission Guidelines</h3> <iframe src="https://services.cognitoforms.com/f/OQ3A98HloUi2D8ZR_2sj-A?id=2" style="position: relative; width: 1px; min-width: 100%; overflow: visible;" frameborder="0" scrolling="no" seamless="seamless" height="795px" width="100%" data-cog-init="true"></iframe>  <h3 style="text-align: left;"> </h3> <h3 style="text-align: left;">Direct Shipping (FedEx):</h3> <div style="text-align: left;"> <p>Please use an overnight delivery service such as FedEx for all NGS samples. A printout of the confirmed quote must be included with the material.</p> <br /> <strong>All direct shipments should be sent to:</strong></div> <div style="text-align: left;"> <p>  <br /> Azenta Life Sciences <br /> Attn: NGS <br /> 111 Corporate Boulevard <br /> Suite H Loading Dock<br /> South Plainfield, NJ 07080 <br /> USA </p> </div> <ul class="list-inline"> <li> <h3><a href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines#dna-seq" class="blue-text">DNA</a> </h3> </li> | <li> <h3><a href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines#rna-seq" class="blue-text">RNA</a> </h3> </li> | <li> <h3><a href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines#protein" class="blue-text">Protein</a> </h3> </li> | <li> <h3><a href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines#extraction-req" class="blue-text">Extraction Requirements</a> </h3> </li> | <li> <h3><a href="/Public/Resources/Sample-Submission-Guidelines/Next-Generation-Sequencing-Sample-Submission-Guidelines#sample-quality" class="blue-text">Sample Quality Control Assessment</a>  </h3> </li> </ul> </div>  <div class="container text-left"> <h1 id="dna-seq">DNA Sample Submission Guidelines</h1> <em>DNA can be concentrated free of charge via SpeedVac<sup>TM</sup>. Additional charges and turnaround time will apply for concentration via column or other methods.</em> <h3>Whole Genome Sequencing / Low-Pass WGS / Shotgun Sequencing</h3> <ul> <li><strong>Sample Type</strong>: Genomic DNA (RNA-free)</li> <li><strong>Sample Purity (OD260/280):</strong> 1.8-2.0</li> <li><strong>Recommended Quantity:</strong> >1.5 <span sc-part-of="field" class="scWebEditInput" id="fld_3AF7A299B86E46E5B6644C6F3DC2ABB3_3BC76BA4EE5C433DB9FDA754AA84AC64_en_1_1b8adf5717544141b1f8af51d4279050_624_edit" contenteditable="false">µg</span>, >20 ng/<span sc-part-of="field" class="scWebEditInput" id="fld_3AF7A299B86E46E5B6644C6F3DC2ABB3_3BC76BA4EE5C433DB9FDA754AA84AC64_en_1_1b8adf5717544141b1f8af51d4279050_624_edit" contenteditable="false">µ</span>l</li> <li><strong>Minimum Amount:</strong> 500 ng</li> <li><strong>Resuspension Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Dry Ice, overnight</li> </ul> <h3>Single-Cell ATAC-Seq</h3> <ul> <li><strong>Sample Type:</strong> Frozen cell suspension according to Azenta-supplied protocol, submitted in duplicate</li> <li><strong>Sample Viability:</strong> >90% post-thaw</li> <li><strong>Recommended Quantity:</strong> >1M cells in 1 mL</li> <li><strong>Minimum Quantity:</strong> 50,000 cells in 500 µL</li> <li><strong>Resuspension Buffer:</strong> Appropriate cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight </li> </ul> <h3>ChIP-Seq</h3> <ul> <li><strong>Sample Type:</strong> ChIP DNA (RNA-free)</li> <li><strong>Sample Purity (OD 260/280):</strong> 1.8-2.0</li> <li><strong>Recommended Quantity:</strong> >50 ng</li> <li><strong>Minimum Amount:</strong> 10 ng</li> <li><strong>DNA Fragment Length:</strong> 100-800 bp; 200-600 bp (Main peak)</li> <li><strong>Resuspension Buffer:</strong> Water, BE, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight </li> </ul> <h3> Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment </h3> <ul> <li><strong>Sample Type:</strong> Genomic DNA (RNA-free)</li> <li><strong>Sample Purity (OD260/280):</strong> 1.8-2.0</li> <li><strong>Recommended Quantity:</strong> >1.5 µg, >20 ng/µl</li> <li><strong>Minimum Amount:</strong> 500 ng</li> <li><strong>Resuspension Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3> Custom TargetGxOne™ Amplicon Sequencing </h3> <ul> <li><strong>Sample Type:</strong> Genomic DNA</li> <li><strong>Sample Purity (OD260/280):</strong> 1.8-2.0</li> <li><strong>Recommended Quantity:</strong> >1 µg, >20 ng/µl</li> <li><strong>Minimum Amount:</strong> 500 ng</li> <li><strong>Resuspension Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3> 16S-EZ & ITS-EZ</h3> <ul> <li><strong>Sample Type:</strong> Genomic DNA</li> <li><strong>Sample Purity (OD260/280):</strong> 1.8-2.0</li> <li><strong>Concentration:</strong> 20 ng/µl</li> <li><strong>Minimum Amount:</strong> 500 ng</li> <li><strong>Resuspension Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>Pre-Made Sequencing Libraries</h3> <ul> <li><strong>Recommended Quantity:</strong> >15 nM</li> <li><strong>Volume:</strong> ≥20 ul</li> <li><strong>Resuspension Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>genoTYPER-NEXT</h3> <ul> <li><strong>Sample Type:</strong> Fresh/frozen cell pellet, washed in PBS </li> <li><strong>Recommended Quantity:</strong> ≥10<sup>6</sup> cells</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <ul> <li><strong>Sample Type:</strong> DNA</li> <li><strong>Recommended Quantity:</strong> 3 ug</li> <li><strong>Resuspension Buffer:</strong> Tris Buffer</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>Amplicon-EZ</h3> <ul> <li><strong>Sample Type: </strong>Purified PCR products</li> <li><strong>Sample Purity (OD260/280):</strong> 1.8-2.0</li> <li><strong>Concentration:</strong> 20 ng/µl</li> <li><strong>Minimum Amount:</strong> 500 ng</li> <li><strong>Resuspension Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> <li><strong>Shipment Method:</strong> Room temperature/blue ice/dry ice, overnight</li> </ul> <h3>Plasmid-EZ</h3> <ul> <li><strong>Sample Type:</strong> Purified plasmid DNA</li> <li><strong>Size:</strong> Up to 25 kb; if larger than 25 kb, please reach out to our NGS experts</li> <li><strong>Minimum Amount:</strong> 500 ng (we recommend using a dsDNA quantification assay such as Qubit™ or PicoGreen™); at least 2 μg of plasmid DNA is required for downstream plasmid DNA preparation</li> <li><strong>Concentration:</strong> Normalized to 50 ng/µL</li> <li><strong>Purity (A260/280):</strong> 1.8-2.0 </li> <li><strong>Buffer:</strong> Water, EB, or low TE (<0.1 mM EDTA)</li> </ul> <h3>Precision Antibody Discovery</h3> <p>For phage display libraries:</p> <ul> <li><strong>Sample Type</strong>:  Amplicon DNA</li> <li><strong>Sample Purity (OD260/280)</strong>:  ~1.8-2.0 </li> <li><strong>Concentration</strong>:  ≥50 ng/µl</li> <li><strong>Recommended Quantity</strong>: ≥2 µg </li> <li><strong>Minimum Quantity</strong>:  ≥500 µg</li> <li><strong>Resuspension Buffer</strong>:  RNA-free water in TE (<0.1 mM EDTA) </li> <li><strong>Shipment Method</strong>:  Dry ice, overnight</li> </ul> <p>For <em>in vivo</em> samples, we can accept total RNA or perform RNA extraction from frozen cell pellets. Please see the <a href="#rna-seq">RNA</a> or <a href="#extraction-req">Extraction Requirements</a> sections for further details. </p> <div><a href="#backtoTop">Back to Top</a></div> <hr /> <h1 id="rna-seq">RNA Sample Submission Guidelines</h1> <p>We now offer RNA Stabilization Tubes. Ship RNA safely at ambient temperature. <span style="text-decoration: underline;"><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://3478602.fs1.hubspotusercontent-na1.net/hubfs/3478602/13031-SD 0222 RNA Stabilization Tubes Sell Sheet.pdf" target="_blank">Download</a></span> the information sheet. <span style="text-decoration: underline;"><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry" target="_blank">Contact</a></span> us to receive a free trial.</p> <h3> Standard/Strand-Specific RNA-Seq & Immuno-Profiling</h3> <ul> <li><strong>Sample Type:</strong> Total RNA (DNA-free)</li> <li><strong>Sample Purity (OD260/OD280):</strong> 1.8-2.2</li> <li><strong>Recommended RIN:</strong> ≥6.0</li> <li><strong>Recommended Quantity:</strong> >2 µg, >50 ng/µl</li> <li><strong>Minimum Quantity:</strong> 250 ng</li> <li><strong>Resuspension Buffer:</strong> Nuclease-free water</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>Small RNA-Seq </h3> <ul> <li><strong>Sample Type:</strong> Total RNA (containing small RNA)</li> <li><strong>Sample Purity (OD260/OD280):</strong> 1.8-2.2, DNA-free</li> <li><strong>Recommended RIN:</strong> ≥6.0</li> <li><strong>Recommended Quantity:</strong> ≥2 µg, >50 ng/µl</li> <li><strong>Resuspension Buffer:</strong> Nuclease-free water</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <ul> <li><strong>Sample Type:</strong> Purified small RNA</li> <li><strong>Sample Purity (OD260/OD280):</strong> 1.8-2.2, DNA-free</li> <li><strong>Recommended RIN:</strong> ≥6.0</li> <li><strong>Recommended Quantity:</strong> >50 ng, >50 ng/µl</li> <li><strong>Resuspension Buffer:</strong> Nuclease-free water</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3> </h3> <h3>Ultra-Low Input RNA-Seq </h3> <p>Cell number and buffer clearly indicated in Azenta Sample Submission Form</p> <ul> <li><strong>Sample Type:</strong> Cells in solution or small amounts of total RNA</li> <li><strong>Recommended Quantity:</strong> >1,000 cells/sample in Trizol or 20 ng of total RNA</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>Single-Cell RNA-Seq & Single-Cell Immuno-Profiling</h3> <ul> <li><strong>Sample Type:</strong> Frozen cell suspension according to Azenta-supplied protocol, submitted in duplicate; methanol-fixed cells per 10x Genomics<sup>®</sup> guidelines are also accepted</li> <li><strong>Sample Viability:</strong> >90% post-thaw</li> <li><strong>Recommended Quantity:</strong> >1M cells in 500 µL</li> <li><strong>Minimum Quantity:</strong> 50,000 cells in 500 µL</li> <li><strong>Resuspension Buffer:</strong> Appropriate cryopreservation media (e.g., cell culture media supplemented with 20% FBS and 10% DMSO)</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>High-Throughput Gene Expression (HT-GEx) Screening</h3> <ul> <li><strong>Recommended Quantity: </strong>Frozen cell lysates, 5,000 - 20,000 cells in 15µl of lysis buffer</li> <li><strong>Recommended Lysis Buffer: </strong>QIASeq UPX Cell Lysis Buffer (#333076) or equivalent</li> <li><strong>Shipment Method: </strong>Dry ice, overnight</li> <li><strong>Sample Container: </strong>PCR-clean 96-well PCR-plate -80°C dry-ice resistant and foil sealed</li> <li><strong>Sample Type:</strong></li> <li style="margin-left:2em;">Cell lysate</li> <li style="margin-left:2em;">Purified RNA (please contact us for details)</li> </ul> <h3>PacBio Iso-Seq</h3> <ul> <li><strong>Sample Type:</strong> Total RNA</li> <li><strong>Sample Purity (OD260/280):</strong> 1.8 - 2.2</li> <li><strong>Recommended RIN:</strong> ≥9.0</li> <li><strong>Recommended Quantity:</strong> ≥2µg</li> <li><strong>Recommended Volume:</strong> 10 - 20µL</li> <li><strong>Shipment Method:</strong> Dry ice, overnight</li> </ul> <h3>Precision Antibody Discovery</h3> <p>For <em>in vivo</em> samples:</p> <ul> <li><strong>Sample Type</strong>:  Total RNA</li> <li><strong>Sample Purity (OD260/OD280)</strong>:  1.8-2.0</li> <li><strong>Recommended RIN</strong>:  ≥6.0</li> <li><strong>Recommended Quantity</strong>: ≥1 µg</li> <li><strong>Minimum Quantity</strong>:  ≥250 ng</li> <li><strong>Resuspension Buffer</strong>:  Nuclease-free water</li> <li><strong>Shipment Method</strong>: Dry ice, overnight</li> </ul> <p>We also accept frozen cell pellets for RNA extraction. Please see the <a href="#extraction-req">Extraction Requirements</a> section for further details or contact us to inquire about other types of starting material. </p> <p>For phage display libraries, we can accept amplicon DNA. Please see the <a href="#dna-seq">DNA</a> section for further details.</p> <div><a href="#backtoTop">Back to Top</a></div> <hr /> <h1 id="protein">Protein Sample Submission Guidelines</h1> <h3>Olink Proteomics<sup>®</sup></h3> <p> <strong>Sample Preparation:</strong></p> <ul> <li><strong>Recommended Quantity:</strong> 30µl - 100µl at 0.5 µg/µL – 1.0 µg/µL of total protein concentration</li> <li><strong>Minimum Amount:</strong> 20µl</li> <li><strong>Shipping Method:</strong>Dry ice</li> <li><strong>Sample Container:</strong>PCR-clean tubes or 96-well PCR plate  -80°C dry-ice resistant and easily resealable</li> <li><strong>Sample Type:</strong>EDTA-plasma, interstitial fluids, cell lysate, microdialysis fluids, microvesicles, citrate-plasma, dried blood spots, synovial fluids, final needle biopsies, urine, saliva, tissue lysates, serum, cebrospinal fluids, cell culture media, heparin-plasma, plaque extracts</li> </ul> <div><a href="#backtoTop">Back to Top</a></div> <hr /> <h1 id="extraction-req">Extraction Requirements</h1> <span style="font-size: 13px;"><em>All extractions are performed to best of Azenta’s ability and the Client is charged for work performed regardless of outcome. Additional charges for procurement of kits may apply for select extractions. </em></span><em style="font-size: 13px;"><span style="font-weight: 700;">Please Note:</span> </em><em style="font-size: 13px;">we do not accept samples above BSL 2 and we require prior notification for pathogenic samples. </em> <h3>FFPE Samples</h3> <ul> <li><strong>Recommended Quantity:</strong> ≥4 FFPE curls recommended</li> <li><strong>Minimum Quantity:</strong> 2 FFPE curls</li> <li><strong>Thickness:</strong> 5µm-20 µm</li> <li><strong>Area:</strong> >150 mm<sup>2</sup></li> <li><strong>Shipping Method:</strong> Room temperature/blue ice</li> </ul> <h3>Cell pellets</h3> <ul> <li><strong>Eukaryotic:</strong></li> <li style="margin-left:2em;"><strong>Recommended Quantity:</strong> 10<sup>6</sup> cells</li> <li style="margin-left:2em;"><strong>Minimum Quantity:</strong> 10<sup>4</sup> cells</li> <li><strong>Microorganism:</strong></li> <li style="margin-left:2em;"><strong>Recommended Quantity:</strong> 10<sup>8</sup> cells</li> <li style="margin-left:2em;"><strong>Minimum Quantity:</strong> 10<sup>6 </sup> cells</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>Fresh Frozen Tissue</h3> <ul> <li><strong>Recommended Quantity:</strong> 30 mg</li> <li><strong>Minimum Quantity:</strong> 2 mg</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>PacBio Blood</h3> <ul> <li><strong>Recommended Quantity:</strong> 10 mL</li> <li><strong>Minimum Quantity:</strong> 3 mL</li> <li><strong>Sample Quality:</strong> Collect blood in tubes containing EDTA and store at -70°C</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>PacBio Mammalian Cells</h3> <ul> <li><strong>Recommended Quantity:</strong> 2x10<sup>7</sup> cells</li> <li><strong>Minimum Quantity:</strong> 1x10<sup>7</sup> cells</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>PacBio Yeast Cells</h3> <ul> <li><strong>Recommended Quantity:</strong> 7x10<sup>9</sup> cells</li> <li><strong>Minimum Quantity:</strong> 3.5x10<sup>9</sup> cells</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>PacBio Bacterial Cells</h3> <ul> <li><strong>Recommended Quantity:</strong> 2.2x10<sup>10</sup> cells</li> <li><strong>Minimum Quantity:</strong> 1x10<sup>10</sup> cells</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>PacBio Fresh Frozen Tissue</h3> <ul> <li><strong>Recommended Quantity:</strong> 100 mg</li> <li><strong>Minimum Quantity:</strong> 50 mg</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>PacBio Viral Particles</h3> <ul> <li><strong>Recommended Quantity:</strong> 1x10<sup>13</sup> vg in <1mL</li> <li><strong>Minimum Quantity:</strong> 1x10<sup>12</sup> vg in <1mL</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>Viral Particles</h3> <ul> <li><strong>Recommended Quantity:</strong> 1x10<sup>12</sup> vg in <1mL</li> <li><strong>Minimum Quantity:</strong> 1x10<sup>11</sup> vg in <1mL</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>Whole Blood</h3> <ul> <li><strong>Recommended Quantity:</strong> 2 tubes/sample</li> <li><strong>Recommended Volume (DNA Extraction):</strong> 500 µl/tube</li> <li><strong>Recommended Volume (RNA Extraction):</strong> 2.5 mL (whole blood, PAXgene Blood RNA Tube)</li> <li><strong>Shipping Method:</strong> Fresh: Blue ice, frozen: Dry ice, overnight</li> </ul> <h3>Plasma/Serum</h3> <ul> <li><strong>Recommended Quantity:</strong> 2 tubes/sample</li> <li><strong>Recommended Volume:</strong> 10 mL/tube</li> <li><strong>Minimum Volume:</strong> 5 mL/tube</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <h3>Stool</h3> <ul> <li><strong>Fecal pellet samples (solid, no buffer)</strong></li> <li><strong>Recommended Quantity:</strong> 100 mg</li> <li><strong>Minimum Quantity:</strong> 50 mg</li> <li><strong>Shipping Method:</strong> Dry ice/blue ice, overnight</li> </ul> <h3>Saliva</h3> <ul> <li><strong>Recommended Quantity:</strong> 1 mL</li> <li><strong>Minimum Quantity:</strong> 500 uL</li> <li><strong>Shipping Method:</strong> Dry ice/blue ice, overnight</li> </ul> <h3>Soil</h3> <ul> <li><strong>Recommended Quantity:</strong> 100 mg</li> <li><strong>Minimum Quantity:</strong> 50 mg</li> <li><strong>Shipping Method:</strong> Room temperature/blue Ice, overnight</li> </ul> <h3>Water</h3> <ul> <li><strong>Recommended Volume:</strong> 100 mL</li> <li><strong>Minimum Volume:</strong> 20 mL</li> <li><strong>Shipping Method:</strong> Room temperature</li> </ul> <h3>Swab</h3> <ul> <li><strong>Recommended Quantity:</strong> 2 tubes/sample; 1 swab/tube</li> <li><strong>Shipping Method:</strong> Room temperature</li> </ul> <h3>Fixed Needle Core Biopsy</h3> <ul> <li><strong>Recommended Quantity:</strong> 4 biopsies</li> <li><strong>Minimum Quantity:</strong> 1 biopsy</li> <li><strong>Recommended Diameter:</strong> 1-2 mm diameter; >5 mm in length</li> <li><strong>Shipping Method:</strong> Room temperature/blue ice</li> </ul> <h3>Snap Frozen Needle Core Biopsy</h3> <ul> <li><strong>Recommended Quantity:</strong> 10 mg</li> <li><strong>Minimum Quantity:</strong> 3 mg</li> <li><strong>Shipping Method:</strong> Dry ice, overnight</li> </ul> <div><a href="#backtoTop">Back to Top</a></div> <p> </p> <hr /> <h1 id="sample-quality">Azenta Sample Quality Control Assessment</h1> <p>Azenta performs QC free of charge with results reported within 24 – 48 hours.</p> <table class="table text-left"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <th> </th> <th>DNA*</th> <th>RNA</th> <th>Pre-Made Libraries</th> </tr> <tr> <td>TapeStation</td> <td> </td> <td><em class="BlueCheck icon-4x fa fa-check font_size_50"></em><br /> </td> <td><em class="BlueCheck icon-4x fa fa-check font_size_50"></em><br /> </td> </tr> <tr> <td>Qubit Assay</td> <td><em class="BlueCheck icon-4x fa fa-check font_size_50"></em> <br /> </td> <td><em class="BlueCheck icon-4x fa fa-check font_size_50"></em> <br /> </td> <td><em class="BlueCheck icon-4x fa fa-check font_size_50"></em> <br /> </td> </tr> </tbody> </table> <p>*For High Molecular Weight DNA, gel electrophoresis will also be performed.</p> <p>For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.</p> <p> Phone: (877) 436-3949 ext. 1<br /> <br /> Email: <a href="mailto:NGS@azenta.com">NGS@azenta.com</a></p> <div> <p> </p> </div> </div> <hr /> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> </div> <ul class="list-unstyled row "> <li class="col-sm-3 col-centered"> <a href="/Public/Services/Next-Generation-Sequencing"> </a> <div class="SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"><a 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Next Generation Sequencing Sample Submission Guidelines - Resources - GENEWIZ