GENEWIZ from Azenta | Quantitative Polymerase Chain Reaction (qPCR)

<!DOCTYPE html>    <html lang="en">  <head> <title>GENEWIZ from Azenta | Quantitative Polymerase Chain Reaction (qPCR)</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="With Azenta Life Sciences’ qPCR services, highly-trained Azenta scientists handle the entire qPCR workflow while leveraging their expertise in assay development – including primer design, optimization, and troubleshooting – to provide high-quality, high-throughput qPCR solutions."> <meta name="keywords" content="qPCR, qPCR analysis, qPCR solution, qpcr assay, qpcr amplicon size, qpcr primer design, assay development, qPCR processing "> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Solutions/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Solutions" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Molecular-Genetics/PCR-Solutions" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Molecular-Genetics/PCR-Solutions" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Molecular-Genetics/PCR-Solutions" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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none;">d</span>PCR)</h1> <div> </div> <p>From gene expression and genotyping to copy number variation (CNV) analysis and viral titer, GENEWIZ Multiomics & Synthesis Solutions offer a versatile polymerase chain reaction (PCR) portfolio that can be tailored to support the needs of your genomics project. Whether through endpoint or real-time detection, our unmatched quantitative PCR (qPCR) or digital PCR (dPCR) capabilities provide you with precise, rapid quantitation of nucleic acid sequences to simplify and advance the path toward your next breakthrough. Reach out to our experts to discuss your specific needs for nucleic acid detection.</p> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/RegisterAccount/Login" target="_blank"><button>Request Quote</button></a>   </div> <div class="dsp-content-box" id="dsp-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing?utm_campaign=2024_05_GEN_SANGER%3A%20Trouble%20Shooting%20Sanger%20Sequencing%20Blog&utm_source=EU%20qPCR%20and%20dPCR%20Page&utm_medium=Right%20Banner%20Ad" target="_blank"><img alt="" src="/-/media/11037-D_Sanger-Troubleshoot_WebBanRight.ashx?la=en&hash=190D69722EB285F5CCBB879F8937014492098206" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner { width: 1170px; background-image: url('/-/media/Images/13008-D_HT-GEx_Web-Ban-Full-Lrg-Mockup_Background.ashx'); 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No matter the size of your sample concentrations, this method offers a wide detection range of target sequences. Select from convenient and affordable predeveloped assays or partner with our assay experts to design a custom assay to meet your project requirements.</p> </div> </div> <div class="epi-service" id="epi-service-2"> <div class="epi-service-content"> <h3> Digital PCR (dPCR)<span style="color: #826ec3;"><sup>NEW</sup> </span></h3> <p>dPCR provides absolute quantitation of nucleic acid sequences without the need for complex standard curves or reference targets. Through micro reactions, dPCR is highly sensitive for increased detection of rare transcripts and low-frequency targets. This endpoint method is optimal for reproducibility and offers straightforward data analysis and interpretation of your results. </p> </div> </div> </div> </div> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .epi-service-options1 { width: 1170px; margin: 0 auto; text-align: center; } .service-container1 { width: 100%; text-align: center; display: flex; justify-content: space-around; flex-wrap: wrap; padding:5px; } .epi-service1 { width: 49%; text-align: center; /*display: inline-block;*/ /*vertical-align: top;*/ padding: 35px 35px 35px 35px ; margin: 4px; position: relative; } .epi-service1 img { height: 150px; padding: 20px 0; } .epi-service1 h3 { margin: 0; } .epi-service1 p { width: 90%; margin: 0 auto; /*margin-bottom: 20px;*/ line-height: 20px; } .epi-service1 a { color: #826ec3; } #epi-service1-1 { background: #ffffff; } #epi-service1-1 span { display: none; } #epi-service1-2 { background: #ffffff; } .epi-service-btn1 { background: #826ec3; border: 2px solid #826ec3; padding: 10px 20px; color: #ffffff; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; margin-top: 20px; } .epi-service-btn1:hover { background: #6959a1; border: 2px solid #6959a1; color: #fff; padding: 10px 20px; } @media only screen and (max-width: 1170px) { .epi-service-options1 { width: 90%; } .epi-service-btn1 { font-size: 10px; } } @media only screen and (max-width: 905px) { .epi-service1 { width: 80%; } } @media only screen and (max-width: 768px) { .service-container1 { display: block; } .epi-service1 { width: 100%; margin: 10px 0; padding: 10px 0 20px 0; } .epi-service-btn1 { position: static; left: 0; right: 0; margin: 0; } .epi-service1 p { margin-bottom: 20px; } } </style> <div class="epi-service-options1" id="epigenomics-service-options1"> <h2 style="text-transform: uppercase;"> Key Applications </h2> <div class="service-container1"> <div class="epi-service1" id="epi-service1-1"> <div class="epi-service-content1"> <h3 style="color: #826ec3; text-align:left;">qPCR</h3> <ul> <li style="text-align:left;">Gene expression</li> <li style="text-align:left;">Copy number variation (with standard curve)</li> <li style="text-align:left;">Confirmatory testing of NGS data</li> <li style="text-align:left;">SNP genotyping and allelic discrimination</li> <li style="text-align:left;">Methylation analysis (CpG)</li> <li style="text-align:left;">Pathogen detection and quality control</li> <li style="text-align:left;">Customized qPCR solutions</li> </ul> </div> </div> <div class="epi-service1" id="epi-service1-2"> <div class="epi-service-content1"> <h3 style="color: #826ec3; text-align:left;">dPCR</h3> <ul> <li style="text-align:left;">Absolute copy number variation (CNV)</li> <li style="text-align:left;">Rare mutation and allelic discrimination</li> <li style="text-align:left;">Low-abundance transcript detection</li> <li style="text-align:left;">Viral titer (copies/mL) </li> <li style="text-align:left;">Vector copy number (copies/genome)</li> <li style="text-align:left;">Circulating tumor DNA (ctDNA) and cell-free DNA (cfDNA) detection in liquid biopsy</li> </ul> </div> </div> </div> </div> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; padding: 0; } .dsp-new-container1 { background: #f0f0f0; padding: 30px 0; } .dsp-new-container1 h2 { margin: 0; color: #000000; } .dsp-new-content1 { width: 1170px; margin: 0 auto; } .dsp-new-content1 p { color: #000000; } .dsp-content-box1 { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #dsp-content-box-left1 { width: 98%; text-align: left; padding: 30px 0; } #dsp-content-box-right1 { width:1%; text-align: right; } .dsp-content-box1 img { width: 400px; } .dsp-content-box1 button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .dsp-content-box button1:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .dsp-new-container1 { text-align: center; margin-top: 0; } .dsp-new-content1 { width: 90%; margin: 0 auto; } .dsp-new-content1 { display: inline-block; } #dsp-content-box-right1, #dsp-content-box-left1 { width: 100%; text-align: center; } #dsp-content-box-right1 { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .dsp-new-content1 { width: 90%; margin: 0 auto; } .dsp-content-box1 img { width: 350px; } } </style> <div class="dsp-new-container1"> <div class="dsp-new-content1"> <div class="dsp-content-box1" id="dsp-content-box-left1"> <h2 style="text-align: center;">PCR for Clinical Research</h2> <p style="text-align: left;">Regulatory guidelines recommend the use of PCR methodology for multiple quality and safety checkpoints throughout the drug development process. For cell and gene therapy products, hyper-sensitive dPCR has largely been used for the evaluation of viral titer as well as quantification of viral vector copy number (VCN) of treated cells for AAV, lentivirus, and retrovirus. <br /> Contact us to learn how our customized PCR solutions can help you advance your clinical research. </p> <p style="text-align: center;"><a rel="noopener noreferrer" href="https://web.genewiz.com/pcr-quote-request" rel="noopener noreferrer" target="_blank"><button>Contact an Expert</button></a></p> </div> </div> </div> <br /> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11036-D_Plasmid-ONT_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; 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}); </script> <div class="process-v1 icon-nav Component-BackGround-Gray margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>PCR Workflow</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-3'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/17001DqPCRdPCRAssayDesign210x110.ashx?h=110&la=en&w=210&hash=3772FFF6E036FCC683229B8B8B53EA43ED38507A" alt="Building" width="210" height="110" title="Assay Development" /> </div> <div class="row"> <h3>1. Assay/Primer Design</h3> </div> <div class="row"> </div> </div> </li>  <li class='col-sm-3'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/17001DqPCRdPCRSamplePrep210x110.ashx?h=110&la=en&w=210&hash=4E1B000492EC8D8C3753C4BBA48BB1BEB4096D4C" alt="Sample Prep" width="210" height="110" title="Sample Prep" /> </div> <div class="row"> <h3>2. Sample Preparation</h3> </div> <div class="row"> </div> </div> </li>  <li class='col-sm-3'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/17001DqPCRdPCRPCRProcessing210x110.ashx?h=110&la=en&w=210&hash=236DDA4A1F8A470187A9AE62DB48F5314F5ED926" alt="PCR Processing" width="210" height="110" title="PCR Processing" /> </div> <div class="row"> <h3>3. PCR Processing</h3> </div> <div class="row"> </div> </div> </li>  <li class='col-sm-3'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/17001DqPCRdPCRDataAnalysis210x110.ashx?h=110&la=en&w=210&hash=EB97557F6BC16F017E6644EC73A5E4EB1D14F54E" alt="Data Analysis" width="210" height="110" title="Data Analysis" /> </div> <div class="row"> <h3>4. Data Analysis & Reporting</h3> </div> <div class="row"> </div> </div> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .main-container { padding: 20px 0; } .workflow-container { width: 95%; margin: 0 auto; margin-top: 40px; } .workflow { width: 100%; margin: 0 auto; text-align: center; } .workflow-step1 { display: inline-block; width: 16%; vertical-align: top; padding: 5px; margin-bottom: 40px; } .workflow-step1 img { width: 250px; margin: 0 auto; margin-bottom: 40px; } .workflow-step1 h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .workflow-step1 p { width: 80%; margin: 0 auto; text-align: center; color:#FFFFFF; } .workflow-icon { text-align: center; } @media only screen and (max-width: 1170px) { .workflow { text-align: left; margin: 0 auto; } .workflow-step1 { width: 16%; } } @media only screen and (max-width: 1000px) { .workflow-step1 { width: 100%; } } </style> <div class="main-container"> <h2 style="text-align: center; margin-bottom: 30px;">Sample Types Accepted</h2> <div class="workflow-container"> <div class="workflow"> <div class="workflow-step1"> <div class="workflow-icon"> <img alt="" src="/-/media/17001DqPCRdPCRViralParticles210x110.ashx?la=en&hash=189D76668E57CADE8AB96CD35E7530ECF2CBD202" /> </div> <h3><strong> Viral Particles</strong></h3>  </div> <div class="workflow-step1"> <div class="workflow-icon"> <img alt="" src="/-/media/17001DqPCRdPCRRNA210x110.ashx?la=en&hash=B825297973D0A842AC4625F8A5C71A3942B4A165" /> </div> <h3><strong> RNA or cDNA</strong></h3> </div> <div class="workflow-step1"> <div class="workflow-icon"> <img alt="" src="/-/media/17001DqPCRdPCRDNA210x110.ashx?la=en&hash=BB6DA5A4DB50EE83FF5CD7227D76FB087CACB948" /> </div> <h3><strong> DNA</strong></h3> </div> <div class="workflow-step1"> <div class="workflow-icon"> <img alt="" src="/-/media/17001DqPCRdPCRCells210x110.ashx?la=en&hash=4F3A386C87A846F64D72D3BEB1D620EDBCEDE8F6" /> </div> <h3><strong> Cell or Tissue</strong></h3> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Gray margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0 "> <div class=" container"> <div class="headline-center"> <h2>Technical Resources</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-6'> <a href='https://www.genewiz.com/en/Public/Resources/Sample-Submission-Guidelines/Molecular-Genetics-Sample-Submission-Guidelines' target='_blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/17001DqPCRdPCRSamplePrep210x110.ashx?h=110&la=en&w=210&hash=4E1B000492EC8D8C3753C4BBA48BB1BEB4096D4C" alt="Building" width="210" height="110" title="Sample Prep" /> </div> <div class="row"> <h3>Sample Submission Guidelines</h3> </div> <div class="row"> </div> </div> </a> </li>  <li class='col-sm-6'> <a href='https://www.genewiz.com/en/Public/Resources/FAQs/FAQs-Molecular-Genetics' target='blank'> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Images/FAQ_210x110_purple.ashx?h=111&la=en&w=211&hash=3E72FE67CF61B960C891CE902CD430B183BB8BF7" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>Frequently Asked Questions</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #f1f0f0; padding: 0; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/MG_qPCR_Blog_US_Web-Tech-Thumb_Blue.ashx?la=en&hash=2A4957D1C2685CDC902644346D94E592804711C4" /> </div> <div class="resource resource-right"> <h3>Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide</h3> <p>This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making. <br /> <a href="https://blog.genewiz.com/ngs-qpcr-or-sanger-sequencing-an-assay-selection-guide?hs_preview=oBDChQvP-61477530694&utm_campaign=2022-03 GEN MG-qPCR&utm_source=Targeted Sequencing&utm_medium=technical resources&utm_content=US" target="_blank"> <button>Read Blog</button> </a> </p> </div> </div> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Related Services</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='https://www.genewiz.com/en/Public/Services/Next-Generation-Sequencing/RNA-Seq' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/RNA-Seq_210x110_purple.ashx?h=111&la=en&w=211&hash=4FC3702FB93E5B9DF653AD3CDCC7AB5F8B7D51F6" alt="Building" width="211" height="111" /> </div> <div class="row"> <h3>RNA-Seq</h3> </div> <div class="row"> </div> 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Sequencing" /> </div> <div class="row"> <h3>AAV ITR Sanger Sequencing</h3> </div> <div class="row"> </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> </div> </div> <ul class="list-inline row process-in process-border-3 "> <li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a href='https://web.genewiz.com/pcr-quote-request' target='_blank'> <div class="process-desc"> <img src="/-/media/Icon_90x90_PlaceOrder.ashx?h=91&la=en&w=91&hash=3867869B79260AFB741049BC250879E6340289C1" alt="Building" width="91" height="91" /> </div> <div class="row"> <h3>Place an Order</h3> <br /> </div> </a> </li><li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a 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GENEWIZ from Azenta | Quantitative Polymerase Chain Reaction (qPCR)