Repertoire Analysis Services - Creative Biolabs

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This analytical method provides detailed insights into how the immune system responds to pathogens and other foreign substances, helping to identify the best weapons against diseases. </p> <p> Repertoire analysis not only aids scientists in developing new drugs but also enhances the accuracy of disease diagnosis, advancing medical technology. By combining phage display with repertoire analysis, <strong>Creative Biolabs</strong> can more efficiently discover and validate high-affinity and high-specificity candidate molecules, significantly promoting the progress of medical research and clinical applications. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Repertoire Analysis Services </h2> <div class="row" style="margin-bottom:30px;"> <div class="col-md-2"> <p class="ServiceShowPic"> <img src="../static/img/1-1-1-2-3-magictm-therapeutic-antibody-discovery-6.jpg" width="135" height="118" loading="lazy" alt="Magic™ BCR Repertoire Analysis" /> </p> </div> <div class="col-md-10"> <p> <a href="magic-bcr-repertoire-analysis.html"><strong>BCR Repertoire Analysis</strong></a> </p> <ul class="ullist"> <li> Comprehensive BCR repertoire from the RNA of either humans or mice. </li> <li> Specific capture primers. </li> <li> High fidelity amplification method. </li> <li> Advanced high-throughput sequencing platform. </li> </ul> </div> </div> <div class="row" style="margin-bottom:30px;"> <div class="col-md-2"> <p class="ServiceShowPic"> <img src="../static/img/1-1-1-2-3-magictm-therapeutic-antibody-discovery-7.jpg" width="120" height="110" loading="lazy" alt="Magic™ TCR Repertoire Analysis" /> </p> </div> <div class="col-md-10"> <p> <a href="magic-tcr-repertoire-sequencing-service.html"><strong>TCR Repertoire Analysis</strong></a> </p> <ul class="ullist"> <li> Comprehensive TCR repertoire from the RNA of either humans or mice. </li> <li> Specific capture primers. </li> <li> High fidelity amplification method. </li> <li> Advanced high-throughput sequencing platform. </li> </ul> </div> </div> <div class="row" style="margin-bottom:30px;"> <div class="col-md-2"> <p class="ServiceShowPic"> <img src="../static/img/1-1-1-2-3-magictm-therapeutic-antibody-discovery-5.jpg" width="100" height="96" loading="lazy" alt="Magic™ Peptide Repertoire Analysis" /> </p> </div> <div class="col-md-10"> <p> <a href="magic-peptide-repertoire-analysis.html"><strong>Peptide Repertoire Analysis</strong></a> </p> <ul class="ullist"> <li> Combined with the phage display platform. </li> <li> Custom peptide binder selection and epitope mapping. </li> <li> Optimized sequencing analysis method. </li> </ul> </div> </div> <div class="row" style="margin-bottom:30px;"> <div class="col-md-2"> <p class="ServiceShowPic"> <img src="../static/img/1-1-1-2-3-magictm-therapeutic-antibody-discovery-3.jpg" width="125" height="105" loading="lazy" alt="Magic™ Antibody Repertoire Analysis" /> </p> </div> <div class="col-md-10"> <p> <a href="Magic-Antibody-Repertoire-Analysis.htm"><strong>Antibody Repertoire Analysis</strong></a> </p> <ul class="ullist"> <li> Integration of next generation sequencing (NGS) and bioinformatics in parallel. </li> <li> Sequencing of diverse antibody repertoires at a rate of millions of clones per hour. </li> <li> High-fidelity analysis of the full-length paired heavy- and light-chains expressed by individual B cells. </li> <li> Analysis of <a href="magic-fab-repertoire-analysis.html">Fab repertoire</a>, <a href="magic-scfv-repertoire-analysis.html">scFv repertoire</a>, and <a href="magic-vhh-repertoire-analysis.html">sdAb repertoire</a> </li> </ul> </div> </div> <div class="row" style="margin-bottom:30px;"> <div class="col-md-2"> <p class="ServiceShowPic"> <img src="../static/img/1-1-1-2-3-magictm-therapeutic-antibody-discovery-4.jpg" width="120" height="103" loading="lazy" alt="Magic™ Bioactive Peptide Discovery" /> </p> </div> <div class="col-md-10"> <p> <a href="Magic-Bioactive-Peptides-Discovery-Service.htm"><strong>Bioactive Peptide Discovery</strong></a> </p> <ul class="ullist"> <li> High-throughput. </li> <li> High affinity. </li> <li> Up to 300 unique binders. </li> <li> Fast result. </li> </ul> </div> </div> <div class="row" style="margin-bottom:30px;"> <div class="col-md-2"> <p class="ServiceShowPic"> <img src="../static/img/1-1-1-2-3-magictm-therapeutic-antibody-discovery-8.jpg" width="130" height="120" loading="lazy" alt="Magic™ Quantitative Immune Repertoire for Small Lymphocytes" /> </p> </div> <div class="col-md-10"> <p> <a href="magic-quantitative-immune-repertoires-profiling-for-small-lymphocytes.html"><strong>Quantitative Immune Repertoire for Small Lymphocytes</strong></a> </p> <ul class="ullist"> <li> Qualitative and quantitative BCR/TCR proﬁling. </li> <li> Special for small lymphocyte populations, such as Ag-speciﬁc, tissue-resident cell subsets, microdissection, or ﬁne needle aspirates. </li> <li> Based on UMIs. </li> <li> Low amount of sample input. </li> </ul> </div> </div> <h2 class="h2_tit page_tab_margin" id="tab3"> Related Tech Platform </h2> <p> <a href="/Phage-Display-Platform.html">Phage display platform</a> is a powerful technology used to generate and screen large libraries of peptides or antibodies. It plays a crucial role in repertoire analysis by providing a diverse set of sequences for further study. The integration of the phage display platform with repertoire analysis provides a robust method for discovering and characterizing high-quality candidate molecules, thereby accelerating advancements in medical research and clinical applications. </p> <h2 class="h2_tit page_tab_margin" id="tab4"> Advantages </h2> <ul class="ullist"> <li> Comprehensive Diversity Analysis </li> <li> High-Throughput Sequencing </li> <li> Enhanced Specificity & Affinity </li> <li> Advanced Bioinformatics </li> <li> Accelerated Drug Development </li> <li> Versatile Applications </li> <li> Customizable Solutions </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab5"> Published Data </h2> <p> Analysis of IgM and IgT Repertoires from <em>Takifugu rubripes</em> </p> <p class="show-center"> <img loading="lazy" src="/static/img/2-3-2-repertoire-analysis-1.jpg" width="749" height="398" alt="Fig. 1 A figure presents the repertoire analysis results of three torafugu. (Fu, et al., 2018)" />Fig. 1 Overall IgM (A) and IgT (B) repertoires across the three torafugu.<sup>1</sup> </p> <p> Scientists analyzed the antibody library of torafugu and obtained 1.2 billion expressed antibody sequences from three healthy adults. The findings revealed convergence and evolution of CDR-H3 sequences in IgM and IgT, and a bias towards specific VDJ combinations in individual fish. Different components of IgM and IgT were also observed, suggesting varied mechanisms for diversity creation in the naïve immune system of fish. </p> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab6"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">Q: How do you ensure the accuracy and reliability of the sequencing data?</span> <p> A: We employ advanced bioinformatics tools and algorithms for data analysis, coupled with stringent quality control measures throughout the sequencing process to ensure high accuracy and reliability. </p> </li> <li> <span class="sFAQTit">Q: What is the typical turnaround time for repertoire analysis services?</span> <p> A: The typical turnaround time ranges from 10 to 12 weeks, depending on the complexity of the project and the volume of samples. </p> </li> <li> <span class="sFAQTit">Q: What types of samples can be used for repertoire analysis?</span> <p> A: We can analyze a variety of sample types, including peripheral blood mononuclear cells (PBMCs), bone marrow, lymph nodes, and tissue biopsies. </p> </li> <li> <span class="sFAQTit">Q: What kind of output data and reports can I expect?</span> <p> A: You will receive a comprehensive report including raw sequencing data, processed data files, and detailed analyses of clonotypes, diversity metrics, and graphical representations of the immune repertoire. </p> </li> <li> <span class="sFAQTit">Q: How do you deal with the amplification biases introduced during library preparation?</span> <p> A: We use unique molecular identifiers (UMIs) during library preparation to accurately quantify and correct amplification biases, ensuring a more accurate representation of the immune repertoire. </p> </li> </ol> <br /> <p> Whether you are involved in drug discovery, disease diagnosis, or immunological research, <strong>Creative Biolabs</strong> provides valuable insights and supports your scientific endeavors. For more information or to discuss your specific requirements, please <span class="inquiry-button">contact us</span>. </p> <div class="reference"> <strong>Reference</strong> <ol class="ollist decimal"> <li> Fu, Xi, et al. "High-throughput sequencing of the expressed torafugu (Takifugu rubripes) antibody sequences distinguishes IgM and IgT repertoires and reveals evidence of convergent evolution." <em>Frontiers in Immunology</em> 9 (2018): 251. </li> </ol> </div><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.</p> <div class="related-sections"> <b>Related Sections</b> <b>Services</b> <ul class="related-sections-ul"> <li><a href="/phage-display-library-construction.html" >Phage Display Library Construction Services</a></li> <li><a href="/phage-display-library-screening.html" >Phage Display Library Screening Services</a></li> <li><a href="/immune-antibody-library-services.html" >Monoclonal Antibody Discovery Services</a></li> <li><a href="/Magic-Bioactive-Peptides-Discovery-Service.htm" >Magic™ Bioactive Peptide Discovery Service</a></li> <li><a href="/yeast-display-based-t-cell-receptor-engineering-service.html" >Yeast Display based T Cell Receptor Engineering Service</a></li> </ul> </div> </div> <div class="col-md-3"> <a name="OnlineInquiry"></a> <div class="rightcontact inquiry_bg_small"> <div class="rig_tit">Online Inquiry</div> <link rel="preconnect" href="https://www.recaptcha.net" /> <style type="text/css">.grecaptcha-badge { 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