Antibody Murinization (Convert to Mouse Ab) Service

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Engineering</a></li><li><a href="/Antibody-Engineering-Services.html">Antibody Conversion</a></li> <li>Murinization (Convert to Mouse Ab)</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Antibody Murinization (Convert to Mouse Ab) Service</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Services</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Published Data</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Resources</span> </div> <script> window.addEventListener('load',function(){ page_tab_margin() page_tab_css(); })</script> <h2 class="h2_tit page_tab_margin" id="tab1"> Monoclonal Antibody Murinization </h2> <p> <strong>Creative Biolabs</strong> is proud to be the first company that offers professional monoclonal antibody murinization service. Relying on our unparalleled expertise in antibody engineering and affinity maturation, monoclonal antibodies with a variety of species background, such as human, rabbit, rat, chicken, bovine and dog can be successfully murinized without specificity switch and significant decrease in affinity. </p> <p> Frequently, human therapeutic antibodies require a mouse counterpart so its efficacy can be tested in rodent animal models. In these cases, murinization of the human antibodies is the most straightforward method to achieve this goal. Based on our extensive experience in antibody humanization, we usually select the best matching murine antibody frameworks and then perform CDR grafting, which is then followed by construction and screening of a unique phage display library with designed mutation positions. In the end, multiple "murinized" mutants with the high affinity retained can be obtained. </p> <p class="ServiceShowPic"> <img loading="lazy" width="650" height="186" src="static/img/Antibody Murinization Service-1.jpg" alt="Monoclonal Antibody Murinization Service" /> </p> <p> After murinization, the "murinized" frameworks greatly decrease their immunogenic in mice as compared to the parental antibody or chimeric murine-human antibody, and thus made them ideal antibodies for pharmacophore studies in rodents. In addition to our unique antibody murinization service, <b>Creative Biolabs</b> is one of the leading companies that is specialized in <a href="/antibody-humanization.html">custom antibody humanization service</a>. </p> <p> Other optional <a href="/antibody-engineering-services.html">antibody engineering services</a>: </p> <ul class="ullist"> <li> <a href="/antibody-affinity-maturation.html">Antibody Affinity Maturation</a> </li> <li> <a href="/Antibody-Stability-Improvement.html">Antibody Stability Improvement</a> </li> <li> <a href="/Simianization-of-Non-monkey-Antibodies.html">Simianization of Non-monkey Antibodies</a> </li> <li> <a href="/Antibody-Caninization-Service.html">Monoclonal Antibody Caninization Service</a> </li> <li> <a href="/Custom-Cysteine-Modification-in-Antibody.html">Custom Cysteine Modification in Antibody</a> </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab2"> Published Data </h2> <ul class="ullist"> <li> <strong>Murinized Anti-IL-1b Antibody</strong> </li> </ul> <p class="show-center"> <img loading="lazy" src="/static/img/1-2-4-11-antibody-murinization-2.jpg" width="450" height="279" alt="" />Fig. 2 Anti‐IL‐1β treatment reduces serum FetA and urinary TNF‐α levels in diabetic DBA/2J mice. (Jana M. Orellana, 2017) </p> <p> The study investigated the effects of Fetuin-A (FetA) and palmitic acid on podocyte death, highlighting the role of interleukin-1 (IL-1) signaling in exacerbating lipotoxicity in podocytes—a key factor in diabetic nephropathy (DN). <em>In vitro</em> experiments demonstrated that both FetA and lipopolysaccharide (LPS) intensified palmitic acid-induced podocyte death, which was strongly linked to an inflammatory response involving the secretion of monocyte chemoattractant protein-1 (MCP-1) and keratinocyte chemoattractant (KC). The murinized anti-IL-1b antibody played a critical role in attenuating the inflammatory and cellular death responses elicited by FetA and palmitic acid. <em>In vivo</em> experiments with diabetic DBA/2J mice treated with the anti-IL1-b antibody for four weeks demonstrated a preventative effect on the increase in serum FetA levels and a significant reduction in urinary tumor necrosis alpha (TNF-a) levels. The study underscores the significance of IL-1b signaling in the pathogenesis of DN, suggesting that inhibiting this pathway could be a promising strategy for the treatment and prevention of DN. The use of murinized antibodies against IL-1b specifically targets this pathway, providing a rationale for more prolonged in vivo studies to explore the therapeutic potential of anti-IL-1b therapy in DN. </p> <div class="reference"> <strong>Reference</strong> <ol class="ollist decimal"> <li> Orellana, Jana M., et al. "Fetuin‐A aggravates lipotoxicity in podocytes via interleukin‐1 signaling." <em>Physiological reports</em> 5.10 (2017): e13287. </li> </ol> </div> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab3"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">What is antibody murinization?</span> <p> Murinization is the process of modifying an antibody derived from a non-mouse species (commonly human) to resemble a mouse antibody while retaining its original antigen-binding specificity. This is achieved by grafting the variable regions of the human antibody onto the constant regions of a mouse antibody. This technique is often used to reduce the immunogenicity of therapeutic antibodies in mouse models during preclinical testing. </p> </li> <li> <span class="sFAQTit">Why is murinized antibody important in research?</span> <p> Murinized antibodies are crucial in biomedical research, particularly in the development and testing of therapeutic antibodies. By using antibodies that are structurally similar to mouse antibodies, researchers can effectively study the efficacy, pharmacokinetics, and immunological properties of these antibodies in mouse models, which are commonly used to mimic human disease states prior to clinical trials. </p> </li> <li> <span class="sFAQTit">How is antibody murinization performed?</span> <p> The process of murinization involves several molecular biology techniques. First, the variable domains of the human antibody (VH and VL) that are responsible for antigen binding are identified and sequenced. These human variable domains are then genetically fused to the constant domains (CH and CL) of a mouse antibody. The resulting chimeric genes are then cloned into an expression vector, which is introduced into a suitable host cell line for antibody production. </p> </li> <li> <span class="sFAQTit">How does murinization affect the efficacy of an antibody in preclinical models?</span> <p> Murinization can affect antibody efficacy in several ways. By replacing the human constant regions with mouse constant regions, the interaction of the antibody with mouse effector cells and complement systems is optimized, potentially improving the efficacy of antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) in mouse models. However, changes in the antibody structure could also impact its binding affinity to the target antigen, which needs to be carefully evaluated during development. </p> </li> <li> <span class="sFAQTit">Can murinized antibodies be used in human clinical trials?</span> <p> Murinized antibodies are generally not used in human clinical trials due to their potential immunogenicity in humans. They are primarily used in preclinical studies to evaluate the biological efficacy of therapeutic antibodies in mouse models. For clinical trials involving humans, fully human or humanized antibodies are preferred as they are less likely to be recognized as foreign by the human immune system. </p> </li> <li> <span class="sFAQTit">What are the main applications of murinized antibodies?</span> <p> The primary application of murinized antibodies is in the preclinical phase of drug development, particularly for the evaluation of therapeutic antibodies in mouse models. These antibodies are used to study the pharmacodynamics, pharmacokinetics, and toxicology of potential therapeutic agents in a biologically relevant system that closely mimics human responses. </p> </li> <li> <span class="sFAQTit">Are there alternatives to murinization in antibody development?</span> <p> Alternatives to murinization include humanization and the development of fully human antibodies using phage display or transgenic mice that are capable of producing human antibodies. Humanization involves modifying a small portion of the non-human antibody to increase its similarity to human antibodies, reducing immunogenicity while retaining high specificity and affinity. Fully human antibodies are derived entirely from human gene sequences, thus minimizing the risk of immunogenicity in humans. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab4"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span><span>Podcasts</span><span>Infographic</span><span>Flyer</span><span>Case study</span><span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/introduction-of-recombinant-antibody.jpg" alt="" /> </div> <a href="https://youtu.be/0-yZ9dTGg18" target="_blank">Introduction of Recombinant Antibody</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/development-of-monoclonal-antibodies.jpg" alt="" /> </div> <a 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loading="lazy" src="../static/img/podcasts-ad.png" width="1700" height="500" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/A-Brief-Introduction-to-Antibodies-1.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/a-brief-introduction-to-antibodies.html">A Brief Introduction to Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/De-Novo-Antibody-Seuqencing-Service-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a 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