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<!DOCTYPE html> <html lang="en" dir="ltr"> <head> <meta charset="utf-8" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-NCKYSBRCP5"></script> <script>window.dataLayer = window.dataLayer || [];function gtag(){dataLayer.push(arguments)};gtag("js", new Date());gtag("set", "developer_id.dMDhkMT", true);gtag("config", "G-NCKYSBRCP5", {"groups":"default","page_placeholder":"PLACEHOLDER_page_location","allow_ad_personalization_signals":false});</script> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <style>.logo a {background-image: url(/dept/drc/dept/drc/themes/custom/bccrc/logo.svg);}</style> <link rel="icon" href="/dept/drc/dept/drc/themes/custom/bccrc/favicon.ico" type="image/vnd.microsoft.icon" /> <link rel="canonical" 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</head> <body class="colour--eggplant not-front path--node page--service"> <a href="#main-content" class="visually-hidden focusable skip-link"> Skip to main content </a> <div class="dialog-off-canvas-main-canvas" data-off-canvas-main-canvas> <div class="main-container"> <header class="page--service page"> <div class="region__header--inner region--inner"> <section id="block-bccrc-breadcrumbs" class="block block--system-breadcrumb-block block-bccrc-breadcrumbs"> <nav class="breadcrumb" role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/dept/drc/dept/drc/">Deeley Research Centre</a> </li> <li> <a href="/dept/drc/dept/drc/services">Services</a> </li> <li> Molecular and Cellular Immunology Core </li> </ol> </nav> </section> <section id="block-bccrc-page-title" class="block block--page-title-block block-bccrc-page-title"> <h1><span>Molecular and Cellular Immunology Core</span> </h1> </section> </div> </header> <section class="region region__highlight"> <div class="region__highlight--inner region--inner"> <div data-drupal-messages-fallback class="hidden"></div> </div> </section> <main role="main"> <a id="main-content" tabindex="-1"></a> <div class="layout-content"> <section class="region region__content"> <div class="region__content--inner region--inner"> <section id="block-bccrc-content" class="block block--system-main-block block-bccrc-content"> <article class="node node--service full"> <div class="field field--body"><p>The Molecular and Cellular Immunology Core (MCIC) is a full service facility specializing in advanced molecular and histological methods including multicolour immunohistochemistry (mcIHC) and immunofluorescence (mcIF), automated image analysis, laser capture microdissection, multispectral flow cytometry, and single-cell sequencing. <br> <br> MCIC supports academic and industry projects both nationally and internationally, ranging from investigator-driven basic research, to correlative studies for clinical trials, to fee-for-service projects with industrial clients.</p></div> </article> <section class="layout layout__expanding layout__parent__node has-expanding-text paragraph paragraph--type--expanding paragraph--view-mode--default" id="services"> <h2 class="section__title" data-expand="field--field_text">Histology</h2> <div class="field field--field_expanding_summary">Histology – Tissue fixation, paraffin embedding, sectioning, mounting, TMA construction, automated mcIHC and mcIF staining (up 8-colour), and laser capture microdissection. <br /> <br /> Imaging – Advanced imaging suite with cutting-edge equipment, including a Vectra 3.0 automated quantitative pathology imaging system, Pannoramic MIDI digital slide scanner, and Vectra Polaris. <br /> <br /> Analysis – Advanced image analysis software, including InForm (Perkin Elmer), HALO (Indica Labs) and QuPath, to quantify morphological and cellular features within tissue samples. <br /> <br /> To inquire about histology services or to request a quote please contact Katy Milne (<a href="mailto:kmilne@bccrc.ca">kmilne@bccrc.ca</a>).</div> <button class="icon" data-expand></button> <div class="field field--field_text"> <p>Please consult the <a href="https://www.bccrc.ca/dept/drc/dept/drc/dept/drc/sites/deelyresearch/files/MCIC%20Histology%20Services%2031Oct24.pdf">MCIC service guide</a> for additional information.</p></div> </section> <section class="layout layout__expanding layout__parent__node paragraph paragraph--type--expanding paragraph--view-mode--default" id="services"> <h2 class="section__title" >Flow Cytometry</h2> <div class="field field--field_expanding_summary">High dimensional flow cytometry – Two Cytek Aurora spectral flow cytometers (up to 48-parameter analyses) and dedicated computer workstations for multispectral data analysis. <br /> <br /> Fluorescence activated cell sorting (FACS) – BD FACSMelody for single cell sorting<br /> <br /> To inquire about flow cytometry services or to request a quote please contact Tyler Dyer (<a href="mailto:tdyer@bccrc.ca">tdyer@bccrc.ca</a>).</div> </section> <section class="layout layout__columns layout__parent__node paragraph paragraph--type--columns paragraph--view-mode--default" id="paragraph--70"> <div class="columns columns--3"> <h2 class="section__title">Multicolour immunohistochemistry</h2> <div class="layout layout__column layout__parent__paragraph paragraph paragraph--type--column paragraph--view-mode--default" id="paragraph--69"> <img loading="lazy" src="/dept/drc/dept/drc/sites/deelyresearch/files/styles/teaser/public/2019-10/24jun14%20IROC019%2018%2020x.jpg?itok=Gvn0p8o2" width="600" height="450" alt="" /> <div class="field--field_paragraph_text"> <p>Tertiary lymphoid structure in high-grade serous carcinoma.</p> <h5><em>(CD21: blue, CD3: green, CD20: red, CD208: black, PNAd: brown,CD8: darker purple)</em></h5> </div> </div> <h2 class="section__title">High dimensional flow cytometry</h2> <div class="layout layout__column layout__parent__paragraph paragraph paragraph--type--column paragraph--view-mode--default" id="paragraph--71"> <img loading="lazy" src="/dept/drc/dept/drc/sites/deelyresearch/files/styles/teaser/public/2022-10/MC_Flow_0.jpg?itok=zcd_Oaad" width="600" height="450" alt="" /> <div class="field--field_paragraph_text"> <p>tSNE projections of immune cell compartments from disaggregated HGSC cells colored by cell type and defined using common phenotypic markers.</p> <h5><em>Laumont CM, Wouters MCA, Smazynski J, Gierc NS, Chavez EA, Chong LC, Thornton S, Milne K, Webb JR, Steidl C, Nelson BH. Single-cell Profiles and Prognostic Impact of Tumor-Infiltrating Lymphocytes Coexpressing CD39, CD103, and PD-1 in Ovarian Cancer. Clin Cancer Res. 2021 Jul 15;27(14):4089-4100. PMID: 33963000.</em></h5> </div> </div> <h2 class="section__title">Multicolour immunofluorescence</h2> <div class="layout layout__column layout__parent__paragraph paragraph paragraph--type--column paragraph--view-mode--default" id="paragraph--72"> <img loading="lazy" src="/dept/drc/dept/drc/sites/deelyresearch/files/styles/teaser/public/2019-10/Picture3.png?itok=m55VhBdT" width="600" height="450" alt="" /> <div class="field--field_paragraph_text"> <p>Tumour infiltrating lymphocytes in high-grade serous carcinoma.</p> <h5><em>(CD155: orange, CD68: magenta, PD-L1: cyan, CD3: yellow, CD8: green, PD-1: red, PanCK+: purple'<em>)</em></em></h5> </div> </div> </div> </section> </section> </div> </section> </div> <a class="top" href="#main-content">Back to top</a> </main> <section class="region region__cta"> <div class="region__cta--inner region--inner"> <section id="block-sitecalltoaction" class="block block--block-contenta8a59224-4648-42d5-b71c-294b3c630d8a block-sitecalltoaction"> <div class="field field--body"><p>BC Cancer Foundation is the fundraising partner of BC Cancer, which includes BC Cancer Research. 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