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In the present study, RT-LAMP was used to develop a rapid and sensitive laboratory diagnostic system for the H5N1 highly pathogenic avian influenza (HPAI). The sensitivity of the system was 0.1-0.01 plaque-forming units per reaction for HPAI-H5N1 viruses belonging to the genetically and antigenically distinct clade 1, represented by A/Vietnam/JP1203/2004, and clade 2, represented by A/Indonesia/JP283/2006. This RT-LAMP sensitivity is 10-fold higher than the sensitivity of standard one-step RT-PCR. By using viral RNAs extracted from avian influenza viruses of H1-H15 hemagglutinin (HA) subtypes and human pathogenic respiratory viruses, it was confirmed that the RT-LAMP system amplifies specifically RNA of the H5 subtype virus. The system detected H5-HA genes in throat swabs collected from humans as well as from wild birds. These results suggest that the present RT-LAMP system is a useful diagnostic tool for surveillance of recent outbreaks of the HPAI-H5N1 virus.","publication_date":"2007,,","publication_name":"Journal of Virological Methods","grobid_abstract_attachment_id":"53986416"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin gene-specific loop-mediated isothermal amplification method","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [35846984]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "control"; window.loswp.useOptimizedScribd4genScript = false; window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":53986416,"attachmentType":"pdf"}"><img alt="First page of “Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin gene-specific loop-mediated isothermal amplification method”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/53986416/mini_magick20190118-27588-uonb4k.png?1547861399" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/images/single_work_splash/adobe_icon.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin gene-specific loop-mediated isothermal amplification method</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="35846984" href="https://independent.academia.edu/nguyentien61"><img alt="Profile image of nguyen tien" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />nguyen tien</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2007, Journal of Virological Methods</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">8 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 34047476; 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if (!viewCountBody) { throw new Error('Failed to find work views element'); } viewCountBody.textContent = `${commaizedViewCount} views`; } catch (error) { // Remove the whole views element if there was some issue parsing. document.getElementById('work-metadata-view-count')?.parentNode?.remove(); throw new Error(`Failed to parse view count: ${viewCount}`, error); } }; // If the DOM is still loading, wait for it to be ready before updating the view count. if (document.readyState === "loading") { document.addEventListener('DOMContentLoaded', () => { updateViewCount(viewCount); }); // Otherwise, just update it immediately. } else { updateViewCount(viewCount); } })();</script></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is a unique gene amplification method that can be completed within 35 min at 62.5 • C. In the present study, RT-LAMP was used to develop a rapid and sensitive laboratory diagnostic system for the H5N1 highly pathogenic avian influenza (HPAI). The sensitivity of the system was 0.1-0.01 plaque-forming units per reaction for HPAI-H5N1 viruses belonging to the genetically and antigenically distinct clade 1, represented by A/Vietnam/JP1203/2004, and clade 2, represented by A/Indonesia/JP283/2006. This RT-LAMP sensitivity is 10-fold higher than the sensitivity of standard one-step RT-PCR. By using viral RNAs extracted from avian influenza viruses of H1-H15 hemagglutinin (HA) subtypes and human pathogenic respiratory viruses, it was confirmed that the RT-LAMP system amplifies specifically RNA of the H5 subtype virus. The system detected H5-HA genes in throat swabs collected from humans as well as from wild birds. These results suggest that the present RT-LAMP system is a useful diagnostic tool for surveillance of recent outbreaks of the HPAI-H5N1 virus.</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":53986416,"attachmentType":"pdf","workUrl":"https://www.academia.edu/34047476/Rapid_diagnosis_of_H5N1_avian_influenza_virus_infection_by_newly_developed_influenza_H5_hemagglutinin_gene_specific_loop_mediated_isothermal_amplification_method"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":53986416,"attachmentType":"pdf","workUrl":"https://www.academia.edu/34047476/Rapid_diagnosis_of_H5N1_avian_influenza_virus_infection_by_newly_developed_influenza_H5_hemagglutinin_gene_specific_loop_mediated_isothermal_amplification_method"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="53986416" data-landing_url="https://www.academia.edu/34047476/Rapid_diagnosis_of_H5N1_avian_influenza_virus_infection_by_newly_developed_influenza_H5_hemagglutinin_gene_specific_loop_mediated_isothermal_amplification_method" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="105810668" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/105810668/An_Updated_Loop_Mediated_Isothermal_Amplification_Method_for_Rapid_Diagnosis_of_H5N1_Avian_Influenza_Viruses">An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="211432125" href="https://independent.academia.edu/%C4%90%E1%BB%A9cL%E1%BB%A3i%C4%90inh">Đức Lợi Đinh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Tropical Medicine and Health, 2011</p><p class="ds-related-work--abstract ds2-5-body-sm">We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new primers showed higher sensitivity and specificity than the previously published RT-LAMP primers and were comparable to the RT-PCR method currently recommended by WHO. These results suggest that our RT-LAMP assay may be a better choice as a diagnostic tool for current H5N1 influenza virus infection.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses","attachmentId":105175096,"attachmentType":"pdf","work_url":"https://www.academia.edu/105810668/An_Updated_Loop_Mediated_Isothermal_Amplification_Method_for_Rapid_Diagnosis_of_H5N1_Avian_Influenza_Viruses","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/105810668/An_Updated_Loop_Mediated_Isothermal_Amplification_Method_for_Rapid_Diagnosis_of_H5N1_Avian_Influenza_Viruses"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="67104931" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/67104931/The_Single_Step_Multiplex_Reverse_Transcription_Polymerase_Chain_Reaction_Assay_for_Detecting_H5_and_H7_Avian_Influenza_A_Viruses">The Single-Step Multiplex Reverse Transcription- Polymerase Chain Reaction Assay for Detecting H5 and H7 Avian Influenza A Viruses</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="68793983" href="https://independent.academia.edu/JuthatipKeawcharoen">Juthatip Keawcharoen</a></div><p class="ds-related-work--metadata ds2-5-body-xs">The Tohoku Journal of Experimental Medicine, 2007</p><p class="ds-related-work--abstract ds2-5-body-sm">Avian influenza (AI) A virus subtypes H5 and H7 cause severe disease in domestic poultry, including chickens and turkeys. Moreover, H5 and H7 AI A viruses can cross the species barrier from poultry to humans. In the present study, we have developed a single-step multiplex reverse transcription-polymerase chain reaction assay (RT-PCR) for detecting H5 and H7 AI A viruses. This assay was applied to the poultry isolates with the aim of establishing a surveillance method to monitor possible transmission to humans. Two subtype-specific primer sets capable of producing PCR products of 157 and 326 base pairs corresponding to AI A virus H5 and H7 subtypes, respectively, were utilized in a one-step and one-tube reaction. The single-step multiplex RT-PCR assay developed in this study was found to be specific for detecting H5 and H7 AI A viruses. No specific amplification bands were detected with total nucleic acids extracted from other influenza hemagglutinin subtypes and other viral pathogens. The sensitivity of this assay was about 10 3 RNA copies/μ l. In conclusion, this novel single-step multiplex RT-PCR is a simple assay with high potential for rapid, specific and cost effective laboratory diagnosis of H5 and H7 AI A virus isolates from clinical specimens of poultry. Avian influenza virus; hemagglutinin; H5 and H7 AI A viruses; single-step multiplex RT-PCR</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"The Single-Step Multiplex Reverse Transcription- Polymerase Chain Reaction Assay for Detecting H5 and H7 Avian Influenza A Viruses","attachmentId":78047951,"attachmentType":"pdf","work_url":"https://www.academia.edu/67104931/The_Single_Step_Multiplex_Reverse_Transcription_Polymerase_Chain_Reaction_Assay_for_Detecting_H5_and_H7_Avian_Influenza_A_Viruses","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/67104931/The_Single_Step_Multiplex_Reverse_Transcription_Polymerase_Chain_Reaction_Assay_for_Detecting_H5_and_H7_Avian_Influenza_A_Viruses"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="102737299" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/102737299/Development_of_reverse_transcription_recombinase_polymerase_amplification_assay_for_avian_influenza_H5N1_HA_gene_detection">Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="260879537" href="https://independent.academia.edu/mohamedamrshalaby">mohamed amr shalaby</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of virological methods, 2015</p><p class="ds-related-work--abstract ds2-5-body-sm">The 2006 outbreaks of H5N1 avian influenza in Egypt interrupted poultry production and caused staggering economic damage. In addition, H5N1 avian influenza viruses represent a significant threat to public health. Therefore, the rapid detection of H5 viruses is very important in order to control the disease. In this study, a qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of haemagglutinin gene of H5 subtype influenza viruses was developed. The results were compared to the real-time reverse transcription polymerase chain reaction (RT-PCR). An in vitro transcribed RNA standard of 970 nucleotides of the haemagglutinin gene was developed and used to determine the assay sensitivity. The developed H5 RT-RPA assay was able to detect one RNA molecule within 7min, while in real-time RT-PCR, at least 90min was required. H5 RT-RPA assay did not detect nucleic acid extracted from H5 negative samples or from other pathogens producing respir...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection","attachmentId":102927409,"attachmentType":"pdf","work_url":"https://www.academia.edu/102737299/Development_of_reverse_transcription_recombinase_polymerase_amplification_assay_for_avian_influenza_H5N1_HA_gene_detection","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/102737299/Development_of_reverse_transcription_recombinase_polymerase_amplification_assay_for_avian_influenza_H5N1_HA_gene_detection"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="25209092" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/25209092/Rapid_and_Highly_Sensitive_Pathotyping_of_Avian_Influenza_A_H5N1_Virus_by_Using_Real_Time_Reverse_Transcription_PCR">Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="48420688" href="https://paulleaderinbrackets.academia.edu/MartinBeer">Martin Beer</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Clinical Microbiology, 2007</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR","attachmentId":45520326,"attachmentType":"pdf","work_url":"https://www.academia.edu/25209092/Rapid_and_Highly_Sensitive_Pathotyping_of_Avian_Influenza_A_H5N1_Virus_by_Using_Real_Time_Reverse_Transcription_PCR","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/25209092/Rapid_and_Highly_Sensitive_Pathotyping_of_Avian_Influenza_A_H5N1_Virus_by_Using_Real_Time_Reverse_Transcription_PCR"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="3788572" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/3788572/Development_of_a_Real_Time_Reverse_Transcriptase_PCR_Assay_for_Type_A_Influenza_Virus_and_the_Avian_H5_and_H7_Hemagglutinin_Subtypes">Development of a Real-Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="4657463" href="https://independent.academia.edu/DavidSSuarez">David S Suarez</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2002</p><p class="ds-related-work--abstract ds2-5-body-sm">A real-time reverse transcriptase PCR (RRT-PCR) assay based on the avian influenza virus matrix gene was developed for the rapid detection of type A influenza virus. Additionally, H5 and H7 hemagglutinin subtypespecific probe sets were developed based on North American avian influenza virus sequences. The RRT-PCR assay utilizes a one-step RT-PCR protocol and fluorogenic hydrolysis type probes. The matrix gene RRT-PCR assay has a detection limit of 10 fg or approximately 1,000 copies of target RNA and can detect 0.1 50% egg infective dose of virus. The H5-and H7-specific probe sets each have a detection limit of 100 fg of target RNA or approximately 10 3 to 10 4 gene copies. The sensitivity and specificity of the real-time PCR assay were directly compared with those of the current standard for detection of influenza virus: virus isolation (VI) in embryonated chicken eggs and hemagglutinin subtyping by hemagglutination inhibition (HI) assay. The comparison was performed with 1,550 tracheal and cloacal swabs from various avian species and environmental swabs obtained from live-bird markets in New York and New Jersey. Influenza virus-specific RRT-PCR results correlated with VI results for 89% of the samples. The remaining samples were positive with only one detection method. Overall the sensitivity and specificity of the H7-and H5-specific RRT-PCR were similar to those of VI and HI.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development of a Real-Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes","attachmentId":31449972,"attachmentType":"pdf","work_url":"https://www.academia.edu/3788572/Development_of_a_Real_Time_Reverse_Transcriptase_PCR_Assay_for_Type_A_Influenza_Virus_and_the_Avian_H5_and_H7_Hemagglutinin_Subtypes","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/3788572/Development_of_a_Real_Time_Reverse_Transcriptase_PCR_Assay_for_Type_A_Influenza_Virus_and_the_Avian_H5_and_H7_Hemagglutinin_Subtypes"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="17119374" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction">Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="36693995" href="https://independent.academia.edu/HarePradhan">Hare Pradhan</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Avian Diseases, 2007</p><p class="ds-related-work--abstract ds2-5-body-sm">Highly pathogenic Avian Influenza (AI) H5N1 viruses have been spreading from Asia since late 2003. Early detection and classification are paramount for control of the disease because these viruses are lethal to birds and have caused fatalities in humans. Here we describe a TaqMan Reverse Transcriptase-Polymerase Chain Reaction Assay for rapid detection of Avian Influenza virus and for H5 subtyping by targeting HA gene of AI viruses. The assay was highly sensitive than RT-PCR and virus isolation in chick embryos. In the present study all samples (field samples) which are positive for HI and RT-PCR were tested by using TaqMan Reverse Transcriptase-Polymerase Chain Reaction Assay for reconfirmation. AI viruses (H5N1) were detected from nine samples which are received from Maharashtra during Avian influenza outbreak in India in 2006. Real-Time PCR assays was also conducted for detection of viral genome in different organs of experimental infected chickens revealed presence of the virus in all organs with high virus concentration in brain, heart, intestine and cloaca. This test allows definitive confirmation of an AI virus as H5 within hours, which is crucial for rapid implementation of control measures in the event of an outbreak.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase–Polymerase Chain Reaction","attachmentId":42316756,"attachmentType":"pdf","work_url":"https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/17119374/Rapid_Detection_of_Highly_Pathogenic_Avian_Influenza_H5N1_Virus_by_TaqMan_Reverse_Transcriptase_Polymerase_Chain_Reaction"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="13383827" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/13383827/An_SYBR_Green_based_real_time_RT_PCR_assay_for_the_detection_of_H5_hemagglutinin_subtype_avian_influenza_virus">An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32610617" href="https://independent.academia.edu/LesterP%C3%A9rez">Lester Pérez</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Molecular and cellular probes, 2012</p><p class="ds-related-work--abstract ds2-5-body-sm">Increasing diversity among H5 hemagglutinin (HA) subtype avian influenza (AI) viruses has resulted in the need of novel sensitive and specific molecular assays. In this study, an SYBR Green-based real-time reverse transcription-PCR (RRT-PCR) assay was developed for the detection of H5 subtype AI virus. Sequence analysis of the Mexican lineage H5N2 isolates (subgroup B) revealed several mismatches in the primer/hydrolysis probe set reported in the commonly used RRT-PCR assay for the detection of H5 North American lineage. The present assay was designed to circumvent the challenge that these viruses represent for the specific detection of H5 subtype AI viruses. This RRT-PCR assay successfully detected a range of different H5 subtype AI strains from both Eurasian and North American lineages representing different avian H5 HA clades from diverse geographical locations. The sensitivity of the present method was determined by using in vitro-transcribed RNA and 10-fold serial dilutions of ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus","attachmentId":45400974,"attachmentType":"pdf","work_url":"https://www.academia.edu/13383827/An_SYBR_Green_based_real_time_RT_PCR_assay_for_the_detection_of_H5_hemagglutinin_subtype_avian_influenza_virus","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/13383827/An_SYBR_Green_based_real_time_RT_PCR_assay_for_the_detection_of_H5_hemagglutinin_subtype_avian_influenza_virus"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="82025030" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/82025030/Rapid_and_Sensitive_Detection_of_H7N9_Avian_Influenza_Virus_by_Use_of_Reverse_Transcription_Loop_Mediated_Isothermal_Amplification">Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription-Loop-Mediated Isothermal Amplification</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="227293922" href="https://whu-cn.academia.edu/DANHU">DAN HU</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Clinical Microbiology, 2013</p><p class="ds-related-work--abstract ds2-5-body-sm">An epidemic of human H7N9 influenza virus infection recently emerged in China whose clinical features include high mortality and which has also resulted in serious economic loss. The novel reassortant avian-origin influenza A (H7N9) virus which was the causative agent of this epidemic raised the possibility of triggering a large-scale influenza pandemic worldwide. It seemed likely that fast molecular detection assays specific for this virus would be in great demand. Here, we report a one-step reverse transcription–loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of the hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 virus, the minimum detection limit of which was evaluated using in vitro RNA transcription templates. In total, 135 samples from clinical specimens (from either patients or poultry) were tested using this method in comparison with the real-time PCR recommended by the World Health Organization (WHO). Our results showed that (i) RT-LAMP-b...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription-Loop-Mediated Isothermal Amplification","attachmentId":87864154,"attachmentType":"pdf","work_url":"https://www.academia.edu/82025030/Rapid_and_Sensitive_Detection_of_H7N9_Avian_Influenza_Virus_by_Use_of_Reverse_Transcription_Loop_Mediated_Isothermal_Amplification","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/82025030/Rapid_and_Sensitive_Detection_of_H7N9_Avian_Influenza_Virus_by_Use_of_Reverse_Transcription_Loop_Mediated_Isothermal_Amplification"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="6490278" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/6490278/Detection_of_H5_H7_and_H9_subtypes_of_avian_influenza_viruses_by_multiplex_reverse_transcription_polymerase_chain_reaction">Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="10328517" href="https://upm.academia.edu/KhatijahYusoff">Khatijah Yusoff</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Microbiological Research, 2009</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction","attachmentId":48845987,"attachmentType":"pdf","work_url":"https://www.academia.edu/6490278/Detection_of_H5_H7_and_H9_subtypes_of_avian_influenza_viruses_by_multiplex_reverse_transcription_polymerase_chain_reaction","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/6490278/Detection_of_H5_H7_and_H9_subtypes_of_avian_influenza_viruses_by_multiplex_reverse_transcription_polymerase_chain_reaction"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="111913387" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/111913387/Development_and_Bench_Validation_of_Real_Time_Reverse_Transcription_Polymerase_Chain_Reaction_Protocols_for_Rapid_Detection_of_the_Subtypes_H6_H9_and_H11_of_Avian_Influenza_Viruses_in_Experimental_Samples">Development and Bench Validation of Real-Time Reverse Transcription Polymerase Chain Reaction Protocols for Rapid Detection of the Subtypes H6, H9, and H11 of Avian Influenza Viruses in Experimental Samples</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33675429" href="https://independent.academia.edu/DavidSuarez40">David Suarez</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Veterinary Diagnostic Investigation, 2007</p><p class="ds-related-work--abstract ds2-5-body-sm">Real-time reverse transcription polymerase chain reaction (RRT-PCR) is commonly used for the rapid detection, as well as to determine the subtype, of avian influenza viruses (AIVs). There are 16 known serologically distinct hemagglutinin (HA) subtypes of AIV described. Currently, determination of the subtypes of AIVs by RRT-PCR tests has been limited to the H5 and H7 subtypes. In this study, RRT-PCR assays were developed in simplex formats for rapid detection of AIV subtypes H6, H9, and H11. The primers and probes for RRT-PCR were designed from nucleotide sequences of the HA genes, which were either downloaded from GenBank (for H6 and H9) or sequenced for this study. The specificity and sensitivity of the RRT-PCR assays were determined based on the detection of the virus from a proficiency panel consisting of 15 different HA subtypes of AIVs and from serial dilutions of target viral RNA. The subtype-specific RRT-PCR assays were used to detect the virus in cloacal and oropharyngeal s...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development and Bench Validation of Real-Time Reverse Transcription Polymerase Chain Reaction Protocols for Rapid Detection of the Subtypes H6, H9, and H11 of Avian Influenza Viruses in Experimental Samples","attachmentId":109306720,"attachmentType":"pdf","work_url":"https://www.academia.edu/111913387/Development_and_Bench_Validation_of_Real_Time_Reverse_Transcription_Polymerase_Chain_Reaction_Protocols_for_Rapid_Detection_of_the_Subtypes_H6_H9_and_H11_of_Avian_Influenza_Viruses_in_Experimental_Samples","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/111913387/Development_and_Bench_Validation_of_Real_Time_Reverse_Transcription_Polymerase_Chain_Reaction_Protocols_for_Rapid_Detection_of_the_Subtypes_H6_H9_and_H11_of_Avian_Influenza_Viruses_in_Experimental_Samples"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":53986416,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":53986416,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_53986416" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="2612526" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/2612526/Specific_detection_of_H5N1_avian_influenza_A_virus_in_field_specimens_by_a_one_step_RT_PCR_assay">Specific detection of H5N1 avian influenza A virus in field specimens by a one-step RT-PCR assay</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2535968" href="https://vnu-vn.academia.edu/ThanhTo">Thanh L To</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm 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