SEC-MALS: Accurate Determination of Protein Molecular Weight and Confirmation of Polymer State

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href="/P5481-GMP-Monoclonal-Anti-Human-CD3-Antibody-%28OKT3%29-DMF-Filed.html" >Activation Antibodies</a></li> <li><a href="/A1595-ActiveMax®-Cytokines.html" >ActiveMax - Target Specific Beads</a></li> <li><a href="/P4836-ActiveMax%C2%AE-Human-T-cell-ActivationExpansion-CD3CD28-Beads-Premium-grade(Pre-GMP) (Pre-GMP)-DMF-Filed.html" >Activation CD3/CD28 Beads</a></li> <li><a href="/A2107-Solutions-for-T-Cell-Activation-and-Culture.html" >Solutions for T Cell Activation and Culture</a></li> <li><a href="/A2107-Solutions-for-T-Cell-Activation-and-Culture.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Cell Media Additives</h2> <ul> <li><a href="/A2560-ECMproteins.html" >Extracellular Matrix (ECM) Proteins</a></li> <li><a href="/A2145-BME.html" >Mogengel Matrix Basement Membrane Extracts</a></li> <li><a href="/A2612-Laminin.html" >Laminin Proteins</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Cell Culture Medium</h2> <ul> <li><a href="/A2660-T-cell-culture-media.html" >CelThera™ GMP T Cell Expansion Medium</a></li> </ul> </div> <div class="pane_box_link"> <h2>Others</h2> <ul> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> <li><a href="/A1888-Custom-GMP-Services.html" >Custom GMP Protein Services</a></li> <li><a href="/A1751-Key-Raw-Materials-for-Cell-and-Gene-Therapy.html" >More >></a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Kits & Assay Reagents <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box4 pane_boxB"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/Product_hand05.jpg" alt="Kits & Assay Reagents"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>ELISA</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Detection Kits</a></li> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Biomarker Detection Kits</a></li> <li><a href="/A1096-Inhibitor-Screening-Kits.html" >Inhibitor Screening Kits</a></li> <li><a href="/A1078-ELISA-Assay-Kits-for-Therapeutic-Antibodies.html" >Antibody Titer Kits</a></li> <li><a href="/P7571-resDetect%E2%84%A2-GENIUS%E2%84%A2-Nuclease-ELISA-Kit-%28Residue-Testing%29.html" >GENIUS™ Nuclease ELISA Kit</a></li> <li><a href="/search-keywords-protein%20A%20(SuRe).html" >Universal Protein A Quick ELISA Kit</a></li> <li><a href="/search-keywords-AAV-nature-9-w-na9.html" >AAV-related assay kits</a></li> <li><a href="/P5420-Anti-CD19-%28FMC63%29-CAR-Immunogenicity-ELISA-Kit.html" >Immunogenicity Assay Kits</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >Streptavidin (SA) Series Products</a></li> </ul> </div> <div class="pane_box_link"> <h2>qPCR Kits</h2> <ul> <li><a href="/P6109-E-coli-resDNA-Quantitative-Kit-%28qPCR%29.html" >E.Coli Residual DNA Kit</a></li> <li><a href="/P6614-CHO-resDNA-Quantitation-Kit-%28qPCR%29.html" >CHO Residual DNA Kit</a></li> <li><a href="/P6651-HEK293-resDNA-Quantitation-Kit-%28qPCR%29.html" >HEK Residual DNA Kit</a></li> <li><a href="/P6840-resDetect%E2%84%A2-E1A--SV40LTA-resDNA-Quantitation-Kit-%28qPCR%29.html" >E1A & SV40LTA Residual DNA Kit</a></li> <li><a href="/P6842-resDetect%E2%84%A2-Plasmid-resDNA-Quantitation-Kit-%28qPCR%29.html" >pDNA Residual DNA Kit</a></li> <li><a href="/search-keywords-Mycoplasma.html" >Mycoplasma Rapid Detection Kit</a></li> <li><a href="https://www.acrobiosystems.com/A2331-resDetect.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Flow Cytometry</h2> <ul> <li><a href="/P7817-ClinMax%E2%84%A2-Human-TBNK-6-color-Cocktail-Flow-Panel.html" >Human TBNK 6-color Cocktail Flow Panel</a></li> <li><a href="/search-keywords-Th1/Th2%20cytokine.html" >Human Th1/Th2 Cytokine Kit (Flow Cytometry Multiplex Bead Assay)</a></li> <li><a href="/A1128-Pre-Coupling-Magnetic-Beads.html" >Pre-coupled Magnetic Beads</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplexed Flow Cytometry</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >Streptavidin (SA) Series Products</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>ADC Conjugation Kits</h2> <ul> <li><a href="/search-keywords-mmae-nature-9-w-na9.html" >AgLink ADC Kit - MMAE</a></li> <li><a href="/A1210-Targets-for-Antibody-Drug-Conjugates.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>MPCLIA</h2> <ul> <li><a href="/A2038-MPCLIA.html" >MPCLIA Reagents</a></li> </ul> </div> <div class="pane_box_link"> <h2>Other Kits</h2> <ul> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> <li><a href="/A1126-Antigens--Antibodies-for-Diagnostic-Kit-Development.html" >Antibody Pairs</a></li> <li><a href="/A2146-Nuclease-Detection-Kits.html" >RNase / DNase Detection Kits</a></li> <li><a href="/P7954-Recombinant-Factor-C-Endotoxin-Detection-Kit.html" >Recombinant Factor C Endotoxin Detection Kit</a></li> <li><a href="/search-keywords-elispot.html" >ELISpot Assay Kits</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title">Viral Antigens&Antibodies&Kits <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box5 pane_boxB"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/Product_hand02.jpg" alt="Viral Antigens"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>SARS-CoV-2</h2> <ul> <li><a href="/A1226-SARS-CoV-2_spike_mutants.html" >Variants of SARS-CoV-2 SARS-CoV-2 Related Products</a></li> <li><a href="/A1111-SARS-CoV-2-Related-Products.html" >SARS-CoV-2 Related Products</a></li> <li><a href="/A1167-Solutions-for-COVID-19-vaccine-safety-and-immunogenicity-evaluation.html" >Workflow for Vaccine R&D</a></li> </ul> </div> <div class="pane_box_link"> <h2>Varicella Zoster Virus</h2> <ul> <li><a href="/search-keywords-Glycoprotein E (VZV).html" >Glycoprotein E (VZV Antigen)</a></li> <li><a href="/search-keywords-VZV-nature-1-w-na1.html" >Anti-Glycoprotein E Antibody</a></li> <li><a href="/A2374-VZV-vaccine.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Influenza Virus</h2> <ul> <li><a href="/L-141-Hemagglutinin%20(HA).html" >Natural HA Trimer</a></li> <li><a href="/L-332-Neuraminidase%20(NA).html" >Natural NA Tetramer</a></li> <li><a href="/A2523-Flu.html" >HA & NA Antibody</a></li> <li><a href="/A2502-Influenza-Vaccine-Research-Customized-Services.html" >Customized Service</a></li> <li><a href="/A2523-Flu.html" >More >></a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Respiratory Syncytial Virus</h2> <ul> <li><a href="/search-nature-6-molecule-1484-keywords-RSV-w-na6_mo1484.html" >Pre-fusion Glycoprotein</a></li> <li><a href="/search-nature-6-keywords-RSV-molecule-1530-w-na6_mo1530.html" >Post-fusion Glycoprotein</a></li> <li><a href="/search-nature-1-molecule-1627-keywords-RSV-w-mo1627_na1.html" >Anti-RSV Pre-Fusion Antibody</a></li> <li><a href="/search-nature-1-molecule-1724-keywords-RSV-w-na1_mo1724.html" >Anti-RSV Post-fusion Antibody</a></li> <li><a href="/A2222-RSV.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Rabies Virus</h2> <ul> <li><a href="/L-1235-Glycoprotein%20(RABV).html" >Glycoprotein</a></li> <li><a href="/L-1739-Nucleoprotein.html" >Nucleoprotein</a></li> <li><a href="/P7526-Mouse-Anti-RABV-Nucleoprotein-Antibody-IgG-Titer-Serologic-Assay-Kit-%28ELISA%29.html" >Anti-RABV Nucleoprotein Antibody Kit </a></li> <li><a href="/P6055-Mouse-Anti-RABV-Glycoprotein-Antibody-IgG-Titer-Serologic-Assay-Kit-%28ELISA%29.html" >Anti-RABV Glycoprotein Antibody Kit </a></li> <li><a href="/A2438-RABV.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Herpes Virus</h2> <ul> <li><a href="/A2374-VZV-vaccine.html" >VZV</a></li> <li><a href="/A1658-virustop.html#module=ViruStop7" >HSV</a></li> <li><a href="/A1658-virustop.html#module=ViruStop13" >EBV</a></li> <li><a href="/A1658-virustop.html#module=ViruStop19" >HCMV</a></li> <li><a href="/A2642-Herpesviruses.html" >More >></a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Other Viruses</h2> <ul> <li><a href="/A1658-virustop.html#module=ViruStop2" >Influenza virus</a></li> <li><a href="/A1658-virustop.html#module=ViruStop4" >MPXV</a></li> <li><a href="/A1658-virustop.html#module=ViruStop5" >HIV</a></li> <li><a href="/A2438-RABV.html" >RABV</a></li> <li><a href="/A1658-virustop.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>ViruStop Services</h2> <ul> <li><a href="/A1074-Innovation-Lab-Capabilities.html" >More >></a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title">Organoids-related Solutions <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box7 pane_boxB"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/Product_hand070408.jpg" alt="Organoid Toolbox"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Cardiac-related Solutions</h2> <ul> <li><a href="/A2500-Cardiac-related-Solutions-Services.html" >Cardiac Organoids</a></li> <li><a href="/A2500-Cardiac-related-Solutions-Services.html" >Cardiac Cells</a></li> <li><a href="/A2500-Cardiac-related-Solutions-Services.html">Cardiac Services</a></li> <li><a href="/A2500-Cardiac-related-Solutions-Services.html" >Cardiac Case Studies</a></li> <li><a href="/A2398-Disease-and-human-models.html" >More >></a></li> </ul> </div> <div 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<div class="layui-col-md12"> <div class="pane_box_link"> <h2>Liver-related Solutions</h2> <ul> <li><a href="/A2582-Liver-Organoid-Solutions.html" >Liver Organoids</a></li> <li><a href="/A2582-Liver-Organoid-Solutions.html" >Liver Cells</a></li> <li><a href="/A2582-Liver-Organoid-Solutions.html" >Liver Services</a></li> <li><a href="/A2582-Liver-Organoid-Solutions.html" >Liver Case Studies</a></li> <li><a href="/A2398-Disease-and-human-models.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Organoid Culture Reagents</h2> <ul> <li><a href="/A1904-Organoid-Culture.html" >Cytokines for Organoid Culture</a></li> <li><a href="/A2145-BME.html" >Mogengel Matrix for Organoid Culture</a></li> <li><a href="/A2398-Disease-and-human-models.html#MoreRT" >More >></a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Others <i class="layui-icon layui-icon-right"></i> </div> <div 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</div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2> Experimental Instruments and Equipments</h2> <ul> <li><a href="/P7052-Automatic-Nucleic-Acid-Extraction-System.html" >Automatic Nucleic Acid Extraction System</a></li> <li><a href="/P7052-Automatic-Nucleic-Acid-Extraction-System.html" >More >></a></li> </ul> </div> </div> </div> </li> <div class="layui-menu-body-titleA"> Search by Modality </div> <li class="layui-menu-item-parent" > <div class="layui-menu-body-title upA"> <a href="/A2471-Cell-_Therapy-_Solutions.html">Cell Therapy </a><i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel layui-pane2_1 layui-pane2_1_1" > <div class="layui-menu-body-title layui-menu-body-title2"> Cell Therapy </div> <ul> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Discovery and Development <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_1 pane_boxD pane_boxA upA_suow" > <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightA_a.jpg" alt="Discovery & Development"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>CAR Targets</h2> <ul> <li><a href="/A1088-CD19%EF%BC%9Aa-validated-therapeutic-target-for-B-cell-malignancies.html" >CD19 Protein</a></li> <li><a href="/search-keywords-BCMA-molecule-36-w-mo36.html" >BCMA Protein</a></li> <li><a href="/search-keywords-MSLN.html" >MSLN Protein</a></li> <li><a href="/A1971-Hematological-Malignancies.html" >Hematologica Malignancies CAR Targets</a></li> <li><a href="/A1861-Solid-Tumor-CAR-Targets.html" >Solid Tumor CAR Targets</a></li> <li><a href="/A1041-Targets-of-CAR-T-Cell-Therapy.html" >More Targets >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Universal CAR Detection Tool</h2> <ul> <li><a href="/search-keywords-G4S%20Linker.html" >Anti-G4S linker Antibody</a></li> <li><a href="/L-422-Protein%20L.html" >Protein L</a></li> </ul> </div> <div class="pane_box_link"> <h2>Other Target Proteins</h2> <ul> <li><a href="/A1656-MHC-Class-I-Complex-Custom-Service.html" >MHC-peptide Complexes</a></li> <li><a href="/A1536-Proteins-for-Neuroscience.html" >Aneuro - Proteins for Neuroscience</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>General Proteins</h2> <ul> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >SA Series Products</a></li> </ul> </div> <div class="pane_box_link"> <h2>Drug Screening Service</h2> <ul> <li><a href="/A1151-SPR-BLI-analytical-service.html" >SPR /BLI analytical service</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Companion Diagnostics Development</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Manufacturing <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_2 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightA_b.jpg" alt="CMC-Manufacturing"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Cell Culture Products</h2> <ul> <li><a href="/A1798-cell-culture-cytokines.html" >Cell Culture Growth Factors & Antibodies</a></li> <li><a href="/A2660-T-cell-culture-media.html" >CelThera™ GMP T Cell Expansion Medium</a></li> <li><a href="/A1595-ActiveMax®-Cytokines.html" >ActiveMax Cell Activation Beads</a></li> <li><a href="/A2560-ECMproteins.html" >Extracellular Matrix (ECM) Proteins</a></li> <li><a href="/A2097-iPSC-Culturing.html" >CMC Production Process of iPSC-based Cell Therapy</a></li> <li><a href="/A2612-Laminin.html" 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Raw Materials for CGT</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Nucleic Acid Removal Products</h2> <ul> <li><a href="/P5787-GMP-GENIUS%E2%84%A2Nuclease-DMF-Filed.html" >GMP Grade GENIUS™Nuclease </a></li> <li><a href="/P7181-GMP-Salt-Active-GENIUS%E2%84%A2Nuclease.html" >Salt Active GENIUS™Nuclease</a></li> </ul> </div> <div class="pane_box_link"> <h2>Gene Editing Products</h2> <ul> <li><a href="/A1657-CRISPR-Cas-technology.html" >GENPower - Cas Enzymes</a></li> <li><a href="/A1301-enzyme-products.html#mRNA_enzymeA" >mRNA Synthesis Enzymes</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Large-Scale Manufacturing</h2> <ul> <li><a href="/A1751-Key-Raw-Materials-for-Cell-and-Gene-Therapy.html" >GMP-grade Raw Materials</a></li> <li><a href="/A1888-Custom-GMP-Services.html" >GMP Protein Services</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> 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>Residual Cell Culture Materials Detection Kits</a></li> <li><a href="/P6991-resDetect%E2%84%A2-Kanamycin-ELISA-Kit.html" target="_blank" >Residual Antibiotics Detection Kits</a></li> <li><a href="/P7281-resDetect%E2%84%A2-HIV-1-p24-ELISA-Kit-%28Residue-Testing%29.html" target="_blank" >Residual HIV-1 p24 ELISA Kit</a></li> <li><a href="/search-keywords-Mycoplasma.html" target="_blank">Mycoplasma Rapid Detection Kit</a></li> <li><a href="/P7954-Recombinant-Factor-C-Endotoxin-Detection-Kit.html" target="_blank">Recombinant Factor C Endotoxin Detection Kit</a></li> <li><a href="/A2504-resDetect-CGT.html" target="_blank" >CGT CMC Manufacturing Process Residue Detection Solutions</a></li> <li><a href="https://www.acrobiosystems.com/A2331-resDetect.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>CAR Detection Tools</h2> <ul> <li><a href="/A1332-Star-Staining-Proteins-Platform.html" >Star Staining - Fluorescent Labeled Proteins</a></li> <li><a href="/A1118-mAb-for-Anti-CD19-CAR-Detection-Flow-Cytometry-Validated.html" >Anti-FMC63 Antibody</a></li> <li><a href="/L-1788-G4S%C2%A0linker.html" >Anti-G4s Linker Antibody</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> <li><a href="/A1391-IgG-Fc-Proteins%E2%80%94%E2%80%94Ideal-Isotype-Control-for-IgG-Structured-Drug-Development.html" >ComboX Isotype Controls</a></li> </ul> </div> <div class="pane_box_link"> <h2>Potency Assays</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2221-Stable-cell-line.html" >Overexpression cell line - CellInsight</a></li> <li><a href="/A2219-Cell-Line-Customize-Service.html" >Cell Line Customization services</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div 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<h2>Pharmacodynamics Studies</h2> <ul> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li><li><a href="/A1904-Organoid-Culture.html" >Organoid Culture Cytokines</a></li> <li><a href="/A1718-Pre-formed-Fibrils-(PFFs).html" >Pre-formed Fibrils (Aneuro)</a></li> <li><a href="/A2145-BME.html" >Mogengel Matrix for Organoid Culture</a></li> <li><a href="/A2183-DBC.html" >Diagnostic Biochips</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Toxicity Studies</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> </ul> </div> <div class="pane_box_link"> <h2>Immunogenicity Studies</h2> <ul> <li><a href="/A1118-mAb-for-Anti-CD19-CAR-Detection-Flow-Cytometry-Validated.html" >Anti-FMC63 Antibody</a></li> <li><a href="/P5420-Anti-CD19-%28FMC63%29-CAR-Immunogenicity-ELISA-Kit.html" >Immunogenicity Anti-FMC63 ELISA kit</a></li> <li><a href="/A1332-Star-Staining-Proteins-Platform.html" >Star Staining - Fluorescent Labeled Proteins</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Audit & Documentation support</h2> <ul> <li><a href="/A1148-FDA-DMF-Filed-Proteins.html" >FDA DMF Filed Proteins</a></li> <li><a href="/A1557-GMP-Grade-Cytokines.html" >GMP Regulatory Support Files</a></li> </ul> </div> <div class="pane_box_link"> <h2>Companion Diagnostics Tools</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Clinical Research <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_5 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightA_e.jpg" alt="Clinical Studies"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Pharmacokinetics Studies</h2> <ul> <li><a href="/A1332-Star-Staining-Proteins-Platform.html" >Star Staining - Fluorescent Labeled Proteins</a></li> <li><a href="/A1118-mAb-for-Anti-CD19-CAR-Detection-Flow-Cytometry-Validated.html" >Anti-FMC63 Antibody</a></li> <li><a href="/L-1788-G4S%C2%A0linker.html" >Anti-G4s Linker Antibody</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> <li><a href="/A1326-Enzyme-Linked-Immunosorbent-Assay-%28ELISA%29-Service.html" >PK ELISA Development Service</a></li> </ul> </div> <div class="pane_box_link"> <h2>Toxicity Studies</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Immunogenicity Studies</h2> <ul> <li><a href="/A1118-mAb-for-Anti-CD19-CAR-Detection-Flow-Cytometry-Validated.html" >Anti-FMC63 Antibody</a></li> <li><a href="/P5420-Anti-CD19-%28FMC63%29-CAR-Immunogenicity-ELISA-Kit.html" >Immunogenicity Anti-FMC63 ELISA kit</a></li> <li><a href="/A1332-Star-Staining-Proteins-Platform.html" >Star Staining - Fluorescent Labeled Proteins</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> </ul> </div> </div> </div> </li> </ul> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title upA"> Gene Therapy <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel layui-pane2_1 layui-pane2_1_2"> <div class="layui-menu-body-title layui-menu-body-title2"> Gene Therapy </div> <ul> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Discovery and Development <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_1 pane_boxD pane_boxA upA_suow"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightB_a.jpg" alt="Discovery & Development"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Gene Editing</h2> <ul> <li><a href="/A1657-CRISPR-Cas-technology.html" >GENPower - Cas Enzymes</a></li> </ul> </div> <div class="pane_box_link"> <h2>AAV Titer Quantitation</h2> <ul> <li><a href="/P7031-AAV5-Titration-ELISA-Kit-%28Fast%29.html" >AAV5 Titration ELISA Kit (Fast)</a></li> <li><a href="/P7157-AAV5-Titration-ELISA-Kit.html" >AAV5 Titration ELISA Kit </a></li> <li><a href="/P7823-AAV3-Titration-ELISA-Kit-%28Fast%29.html" >AAV3 Titration ELISA Kit (Fast) </a></li> <li><a href="/P7100-AAV3-Titration-ELISA-Kit.html" >AAV3 Titration ELISA Kit</a></li> <li><a href="/P7735-AAV8-Titration-ELISA-Kit-%28Fast%29.html" >AAV8 Titration ELISA Kit (Fast)</a></li> <li><a href="/P7736-AAV8-Titration-ELISA-Kit.html" >AAV8 Titration ELISA Kit</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Companion Diagnostics</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Manufacturing <i class="layui-icon layui-icon-right"></i> </div> <div 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lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Quality Control <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_3 pane_boxD"> <div class="pane_box_img"><img src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightB_c.jpg" alt="CMC-Quality Control" width="100%"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Residual Detection-resDetect</h2> <ul> <li><a href="/A2146-Nuclease-Detection-Kits.html" target="_blank" >Residual Host Cell DNA / RNA Detection Kits</a></li> <li><a href="/search-keywords-Activity.html" target="_blank" >RNase / DNase Detection Kits</a></li> <li><a href="/P7571-resDetect%E2%84%A2-GENIUS%E2%84%A2-Nuclease-ELISA-Kit-%28Residue-Testing%29.html" target="_blank" >GENIUS™ Nuclease Residual Detection Kit</a></li> <li><a href="/P7814-resDetect%E2%84%A2-BSA-ELISA-Kit.html" target="_blank" >Residual BSA ELISA Kit</a></li> <li><a href="/P6991-resDetect%E2%84%A2-Kanamycin-ELISA-Kit.html" target="_blank" >Residual Antibiotics Detection Kits</a></li> <li><a href="/A2504-resDetect-CGT.html" target="_blank" >CGT CMC Manufacturing Process Residue Detection Solutions</a></li> <li><a href="/search-keywords-Mycoplasma.html" target="_blank">Mycoplasma Rapid Detection Kit</a></li> <li><a href="/P7954-Recombinant-Factor-C-Endotoxin-Detection-Kit.html" target="_blank">Recombinant Factor C Endotoxin Detection Kit</a></li> <li><a href="https://www.acrobiosystems.com/A2331-resDetect.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Potency Assays</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> </ul> </div> <div class="pane_box_link"> <h2>AAV Titer Quantitation</h2> <ul> <li><a href="/P7031-AAV5-Titration-ELISA-Kit-%28Fast%29.html" >AAV5 Titration ELISA Kit (Fast)</a></li> <li><a href="/P7157-AAV5-Titration-ELISA-Kit.html" >AAV5 Titration ELISA Kit </a></li> <li><a href="/P7823-AAV3-Titration-ELISA-Kit-%28Fast%29.html" >AAV3 Titration ELISA Kit (Fast) </a></li> <li><a href="/P7100-AAV3-Titration-ELISA-Kit.html" >AAV3 Titration ELISA Kit</a></li> <li><a href="/P7735-AAV8-Titration-ELISA-Kit-%28Fast%29.html" >AAV8 Titration ELISA Kit (Fast)</a></li> <li><a href="/P7736-AAV8-Titration-ELISA-Kit.html" >AAV8 Titration ELISA Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Pre-clinical Research <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_4 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightB_d.jpg" alt="Preclinical Studies"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Pharmacodynamics Studies</h2> <ul> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li><li><a href="/A1904-Organoid-Culture.html" >Organoid Culture Cytokines</a></li> <li><a href="/A1718-Pre-formed-Fibrils-(PFFs).html" >Pre-formed Fibrils (Aneuro)</a></li> <li><a href="/A2145-BME.html" >Mogengel Matrix for Organoid Culture</a></li> <li><a href="/A2183-DBC.html" >Diagnostic Biochips</a></li> </ul> </div> <div class="pane_box_link"> <h2>Security Studies</h2> <ul> <li><a href="/search-keywords-AAV%20antibody%20kit.html" >Anti-AAV Antibody ELISA Kit</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Toxicity Studies</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> </ul> </div> <div class="pane_box_link"> <h2>Audit & Documentation support</h2> <ul> <li><a href="/A1148-FDA-DMF-Filed-Proteins.html" >FDA DMF Filed Proteins</a></li> <li><a href="/A1557-GMP-Grade-Cytokines.html" >GMP Regulatory Support Files</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Companion Diagnostics Tools</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Clinical Research <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_5 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightB_e.jpg" alt="Clinical Studies"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Toxicity Studies</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> </ul> </div> <div class="pane_box_link"> <h2>Security Studies</h2> <ul> <li><a href="/search-keywords-AAV%20antibody%20kit.html" >Anti-AAV Antibody ELISA Kit</a></li> </ul> </div> </div> </div> </li> </ul> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title upA"> <a href="/A2356-Therapeutic-Antibody-Page.html" >Therapeutic Antibodies </a><i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel layui-pane2_1 layui-pane2_1_3"> <div class="layui-menu-body-title layui-menu-body-title2"> Therapeutic Antibodies </div> <ul> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Discovery and Development <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_1 pane_boxD pane_boxA upA_suow"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightC_a.jpg" alt="Discovery & Development"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Target Proteins</h2> <ul> <li><a href="/A1288-Cytokine.html" >Cytokine Targets</a></li> <li><a href="/A974-Immune-Checkpoint-Proteins.html" >Checkpoint Proteins</a></li> <li><a href="/A2117-Agonist-Target-Proteins.html" >Agonist</a></li> <li><a href="/A1290-Transmembrane-Proteins-Platform.html" >FLAG</a></li> <li><a href="/A1536-Proteins-for-Neuroscience.html" >Aneuro</a></li> <li><a href="/A1265-Tumor-microenvironment.html" >Tumor Microenvironment</a></li> <li><a href="/A1222-Bispecific-antibody-targets-and-more.html" >Bispecifc Antibody Targets</a></li> <li><a href="/A1656-MHC-Class-I-Complex-Custom-Service.html" >MHC Complexes</a></li> </ul> </div> <div class="pane_box_link"> <h2>Antibody Screening</h2> <ul> <li><a href="/A716-Biotinylated-Protein.html" >Biotinylated Proteins</a></li> <li><a href="/A1096-Inhibitor-Screening-Kits.html" >Inhibitor Screening Kits</a></li> <li><a href="/A1332-Star-Staining-Proteins-Platform.html" >Star Staining Fluorescent Labeled Proteins</a></li> <li><a href="/A1128-Pre-Coupling-Magnetic-Beads.html" >Pre-coupled Magnetic Beads</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >SA series Products ComboX</a></li> <li><a href="/A1969-Recombinant-Antibody-Services.html" >Recombinant Antibody Expression Services</a></li> <li><a href="/A1151-SPR-BLI-analytical-service.html" >SPR /BLI analytical service</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Fc Proteins</h2> <ul> <li><a href="/A1391-IgG-Fc-Proteins%E2%80%94%E2%80%94Ideal-Isotype-Control-for-IgG-Structured-Drug-Development.html" >IgG Fc Proteins</a></li> <li><a href="/A712-Fc-Receptor-Proteins.html" >Fc Receptor</a></li> <li><a href="/A1394-FcRn%EF%BC%88Neonatal-Fc-Receptor%EF%BC%89.html" >Neonatal Fc Receptor</a></li> <li><a href="/P7366-Human-FcRn-binding-Kit-%28TR-FRET%29.html" >FcRn Binding Kit</a></li> </ul> </div> <div class="pane_box_link"> <h2>Cell Line (CellInsight)</h2> <ul> <li><a href="/A2221-Stable-cell-line.html" >Overexpression cell line</a></li> <li><a href="/A2219-Cell-Line-Customize-Service.html" >Cell Line Customization services</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Companion Diagnostics</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Manufacturing <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_2 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightC_b.jpg" alt="CMC-Manufacturing"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Nucleic Acid Removal</h2> <ul> <li><a href="/P5787-GMP-GENIUS%E2%84%A2Nuclease-DMF-Filed.html" >GMP GENIUS Nuclease</a></li> <li><a href="/P7181-GMP-Salt-Active-GENIUS%E2%84%A2Nuclease.html" >Salt Active GENIUS™Nuclease</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Quality Control <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_3 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightC_c.jpg" alt="CMC-Quality Control"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Residual Detection-resDetect</h2> <ul> <li><a href="/A2146-Nuclease-Detection-Kits.html" >Host DNA/Protein Quantitative Kits</a></li> <li><a href="/P7433-resDetect%E2%84%A2-Universal-Protein-A-Quick-ELISA-Kit-%28Boiling-free%29.html" >Residual Affinity Ligands Detection Kits</a></li> <li><a href="/A1557-GMP-Grade-Cytokines.html#pro" >Residual Cell Culture Materials Detection Kits</a></li> <li><a href="/P7571-resDetect%E2%84%A2-GENIUS%E2%84%A2-Nuclease-ELISA-Kit-%28Residue-Testing%29.html" >GENIUS™ Nuclease Residual Detection Kit</a></li> <li><a href="/P6991-resDetect%E2%84%A2-Kanamycin-ELISA-Kit.html" >Residual Antibiotics Detection Kits</a></li> <li><a href="/A2543-resDetect-Antibody.html" >Therapeutic antibodies CMC Manufacturing Process Residue Detection Solutions</a></li> <li><a href="/search-keywords-Mycoplasma.html" >Mycoplasma Rapid Detection Kit</a></li> <li><a href="/P7954-Recombinant-Factor-C-Endotoxin-Detection-Kit.html" >Recombinant Factor C Endotoxin Detection Kit</a></li> <li><a href="https://www.acrobiosystems.com/A2331-resDetect.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Other Quality Control Products</h2> <ul> <li><a href="/A2221-Stable-cell-line.html" >Overexpression cell line</a></li> <li><a href="/A1391-IgG-Fc-Proteins%E2%80%94%E2%80%94Ideal-Isotype-Control-for-IgG-Structured-Drug-Development.html" >IgG Isotype Control</a></li> <li><a href="/A2154-Tool-enzymes.html" >Enzymes for Antibody Characterization Analysis</a></li> <li><a href="/A1794-MALS-UNcle-Platform.html" >Quality Characterization Analytical Services</a></li> <li><a href="/A2219-Cell-Line-Customize-Service.html" >Cell Line Customization services</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Cytokine Release Detection</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine ELISA Kits</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Preclinical Research <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_4 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightC_d.jpg" alt="Preclinical Studies"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Pharmacokinetics Studies</h2> <ul> <li><a href="/A716-Biotinylated-Protein.html" >Biotinylated Proteins</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >SA series Products ComboX</a></li> <li><a href="/A1089-Anti-idiotypic-Antibodies.html" >Anti-idiotypic Antibodies</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> <li><a href="/A1078-ELISA-Assay-Kits-for-Therapeutic-Antibodies.html" >PK ELISA Assays</a></li> <li><a href="/A1326-Enzyme-Linked-Immunosorbent-Assay-%28ELISA%29-Service.html" >PK ELISA Development Service</a></li> </ul> </div> <div class="pane_box_link"> <h2>Toxicity Studies</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Immunogenicity Studies</h2> <ul> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic & ADA Assay Development Service</a></li> </ul> </div> <div class="pane_box_link"> <h2>Pharmacodynamics Studies</h2> <ul> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li><li><a href="/A1904-Organoid-Culture.html" >Organoid Culture Cytokines</a></li> <li><a href="/A1718-Pre-formed-Fibrils-(PFFs).html" >Pre-formed Fibrils (Aneuro)</a></li> <li><a href="/A2145-BME.html" >Mogengel Matrix for Organoid Culture</a></li> <li><a href="/A2183-DBC.html" >Diagnostic Biochips</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Audit & Documentation support</h2> <ul> <li><a href="/A1148-FDA-DMF-Filed-Proteins.html" >FDA DMF Filed Proteins</a></li> <li><a href="/A1557-GMP-Grade-Cytokines.html" >GMP Regulatory Support Files</a></li> </ul> </div> <div class="pane_box_link"> <h2>Companion Diagnostics Tools</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Clinical Research <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_5 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightC_e.jpg" alt="Clinical Studies"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Pharmacokinetics Studies</h2> <ul> <li><a href="/A716-Biotinylated-Protein.html" >Biotinylated Proteins</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >SA series Products ComboX</a></li> <li><a href="/A1089-Anti-idiotypic-Antibodies.html" >Anti-idiotypic Antibodies</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> <li><a href="/A1078-ELISA-Assay-Kits-for-Therapeutic-Antibodies.html" >PK ELISA Assays</a></li> <li><a href="/A1326-Enzyme-Linked-Immunosorbent-Assay-%28ELISA%29-Service.html" >PK ELISA Development Service</a></li> </ul> </div> <div class="pane_box_link"> <h2>Immunogenicity Studies</h2> <ul> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody ADA Development Service</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Toxicity Studies</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine Release Assay Kits - ClinMax</a></li> <li><a href="/search-keywords-Th1/Th2 cytokine.html" >Multiplex Cytokine Assay Kits</a></li> <li><a href="/A2398-Disease-and-human-models.html" >Organoid Toolbox</a></li> </ul> </div> </div> </div> </li> </ul> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title upA"> Antibody Drug Conjugates <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel layui-pane2_1 layui-pane2_1_4"> <div class="layui-menu-body-title layui-menu-body-title2"> Antibody Drug Conjugates </div> <ul> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Discovery and Development <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_1 pane_boxD pane_boxA upA_suow"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightD_a.jpg" alt="Discovery & Development"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Target Proteins</h2> <ul> <li><a href="/A1210-Targets-for-Antibody-Drug-Conjugates.html#module=kit1" >ADC Drug Targets</a></li> <li><a href="/A1288-Cytokine.html" >Cytokine Targets</a></li> <li><a href="/A974-Immune-Checkpoint-Proteins.html" >Checkpoint Proteins</a></li> <li><a href="/A2117-Agonist-Target-Proteins.html" >Agonist</a></li> <li><a href="/A1290-Transmembrane-Proteins-Platform.html" >FLAG</a></li> <li><a href="/A1536-Proteins-for-Neuroscience.html" >Aneuro</a></li> <li><a href="/A1265-Tumor-microenvironment.html" >Tumor Microenvironment</a></li> <li><a href="/A1222-Bispecific-antibody-targets-and-more.html" >Bispecifc Antibody Targets</a></li> <li><a href="/A1656-MHC-Class-I-Complex-Custom-Service.html" >MHC Complexes</a></li> </ul> </div> <div class="pane_box_link"> <h2>Antibody Screening</h2> <ul> <li><a href="/A716-Biotinylated-Protein.html" >Biotinylated Proteins</a></li> <li><a href="/A1096-Inhibitor-Screening-Kits.html" >Inhibitor Screening Kits</a></li> <li><a href="/A1332-Star-Staining-Proteins-Platform.html" >Star Staining Fluorescent Labeled Proteins</a></li> <li><a href="/A1128-Pre-Coupling-Magnetic-Beads.html" >Pre-coupled Magnetic Beads</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >SA series Products ComboX</a></li> <li><a href="/A1969-Recombinant-Antibody-Services.html" >Recombinant Antibody Expression Services</a></li> <li><a href="/A1151-SPR-BLI-analytical-service.html" >SPR /BLI analytical service</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Fc Proteins</h2> <ul> <li><a href="/A1391-IgG-Fc-Proteins%E2%80%94%E2%80%94Ideal-Isotype-Control-for-IgG-Structured-Drug-Development.html" >IgG Fc Proteins</a></li> <li><a href="/A712-Fc-Receptor-Proteins.html" >Fc Receptor</a></li> <li><a href="/A1394-FcRn%EF%BC%88Neonatal-Fc-Receptor%EF%BC%89.html" >Neonatal Fc Receptor</a></li> <li><a href="/P7366-Human-FcRn-binding-Kit-%28TR-FRET%29.html" >FcRn Binding Kit</a></li> </ul> </div> <div class="pane_box_link"> <h2>ADC Reagents</h2> <ul> <li><a href="/A2584-ADC-Conjugation-Kit.html" >AGLink Conjugation Kit</a></li> <li><a href="/A1210-Targets-for-Antibody-Drug-Conjugates.html" >Proteases for Peptide Linker</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Cell Line (CellInsight)</h2> <ul> <li><a href="/A2221-Stable-cell-line.html" >Overexpression cell line</a></li> <li><a href="/A2219-Cell-Line-Customize-Service.html" >Cell Line Customization services</a></li> </ul> </div> <div class="pane_box_link"> <h2>Companion Diagnostics</h2> <ul> <li><a href="/A2176-IHC-antibody.html" >IHC Antibodies</a></li> <li><a href="/A2246-Immunohistochemistry-%28IHC%29-Kit.html" >Immunohistochemistry (IHC) Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Manufacturing <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_2 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightD_b.jpg" alt="CMC-Manufacturing"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Nucleic Acid Removal</h2> <ul> <li><a href="/P5787-GMP-GENIUS%E2%84%A2Nuclease-DMF-Filed.html" >GMP GENIUS Nuclease</a></li> <li><a href="/P7181-GMP-Salt-Active-GENIUS%E2%84%A2Nuclease.html" >Salt Active GENIUS™Nuclease</a></li> </ul> </div> <div class="pane_box_link"> <h2>Conjugation Kits</h2> <ul> <li><a href="/A2584-ADC-Conjugation-Kit.html" >AGLink Conjugation Kit</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Quality Control <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_3 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightD_c.jpg" alt="CMC-Quality Control"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Residual Detection-resDetect</h2> <ul> <li><a href="/A2146-Nuclease-Detection-Kits.html" >Host DNA/Protein Quantitative Kits</a></li> <li><a href="/P7433-resDetect%E2%84%A2-Universal-Protein-A-Quick-ELISA-Kit-%28Boiling-free%29.html" >Residual Affinity Ligands Detection Kits</a></li> <li><a href="/A1557-GMP-Grade-Cytokines.html#pro" >Residual Cell Culture Materials Detection Kits</a></li> <li><a href="/P7571-resDetect%E2%84%A2-GENIUS%E2%84%A2-Nuclease-ELISA-Kit-%28Residue-Testing%29.html" >GENIUS™ Nuclease Residual Detection Kit</a></li> <li><a href="/search-keywords-Mycoplasma.html" >Mycoplasma Rapid Detection Kit</a></li> <li><a href="/P7954-Recombinant-Factor-C-Endotoxin-Detection-Kit.html" >Recombinant Factor C Endotoxin Detection Kit</a></li> <li><a href="https://www.acrobiosystems.com/A2331-resDetect.html" >More >></a></li> </ul> </div> <div class="pane_box_link"> <h2>Other Quality Control Products</h2> <ul> <li><a href="/A2221-Stable-cell-line.html" >Overexpression cell line</a></li> <li><a href="/A1391-IgG-Fc-Proteins%E2%80%94%E2%80%94Ideal-Isotype-Control-for-IgG-Structured-Drug-Development.html" >IgG Isotype Control</a></li> <li><a href="/A2154-Tool-enzymes.html" >Enzymes for Antibody Characterization Analysis</a></li> <li><a href="/A1794-MALS-UNcle-Platform.html" >Quality Characterization Analytical Services</a></li> <li><a href="/A2219-Cell-Line-Customize-Service.html" >Cell Line Customization services</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>ADC Payload Evaluation</h2> <ul> <li><a href="/A2226-Anti-payload-antibodies.html" >Anti-payload Antibodies</a></li> </ul> </div> <div class="pane_box_link"> <h2>Antibody Enzymes</h2> <ul> <li><a href="/A1301-enzyme-products.html" >Antibody Characterization</a></li> </ul> </div> </div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Cytokine Release Detection</h2> <ul> <li><a href="/A1781-Cytokine-detection-ELISA-kits.html" >Cytokine ELISA Kits</a></li> </ul> </div> </div> </div> </li> <li class="layui-menu-item-parent" lay-options="{type: 'parent'}"> <div class="layui-menu-body-title"> Pre-clinical Research <i class="layui-icon layui-icon-right"></i> </div> <div class="layui-menu-body-panel pane_box pane_box2_4 pane_boxD"> <div class="pane_box_img"><img width="100%" src="https://www.acrobiosystems.com/static/main/images/HomePhotoA/home_rightD_d.jpg" alt="Preclinical Studies"></div> <div class="layui-col-md12"> <div class="pane_box_link"> <h2>Pharmacokinetics Studies</h2> <ul> <li><a href="/A716-Biotinylated-Protein.html" >Biotinylated Proteins</a></li> <li><a href="/A1670-Streptavidin(SA)-Series-Products.html" >SA series Products ComboX</a></li> <li><a href="/A2226-Anti-payload-antibodies.html" >Anti-payload Antibodies</a></li> <li><a href="/A1089-Anti-idiotypic-Antibodies.html" >Anti-idiotypic Antibodies</a></li> <li><a href="/A1320-Anti-Idiotypic-Antibody.html" >Anti-idiotypic Antibody Development Service</a></li> <li><a href="/A1078-ELISA-Assay-Kits-for-Therapeutic-Antibodies.html" >PK ELISA Assays</a></li> <li><a href="/A1326-Enzyme-Linked-Immunosorbent-Assay-%28ELISA%29-Service.html" >PK ELISA Development Service</a></li> </ul> </div> <div class="pane_box_link"> <h2>Pharmacodynamics Studies</h2> <ul> <li><a 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The quality and activity of the recombinant protein can have a tremendous impact on the success and even cycle of antibody-drug development.</p><p class="public_text_dec">Recombinant protein antigens must maintain correct folding and consistent conformation with the native proteins. Therefore, ensuring the natural activity of the protein antigens and minimal batch-to-batch inconsistency, will ensure a high success rate of drug development. As an industry leader in recombinant proteins for drug development, ACROBiosystems is committed to "Where Proteins and Innovation Advance Biomedicine." Our company's commitment extends beyond recombinant protein design, expression, and production process of strict control into an established, accurate, and reliable molecular weight determination analysis method. Our methods can accurately characterize the aggregation state of recombinant proteins under physiological conditions in solution and guide the design, production process optimization, and quality control of recombinant protein antigen products.</p></div><div class="productSection"><div class="productTit">Protein Molecular Weight Analysis Technology Platform</div><p class="public_text_dec" style="font-weight:bold;">Several methods are used to analyze molecular protein weight, including:</p><p class="public_text_dec">(1) An interpolation method based on SEC-HPLC (High-Performance Molecular Sieve Liquid Chromatography) to estimate the retention time;</p><p class="public_text_dec">(2) Molecular weight absolute quantification based on SEC-MALS (Multi-angle Laser Scattering).</p><p class="public_text_dec" style="margin-top:20px; margin-bottom:10px;">Both techniques can determine the molecular weight of proteins in solution, but there are some differences in principle. The similarities and differences between the two techniques are as follows:</p><div class="public_img_text_box public_img_l_r layui-col-space10"><table class="public_table layui-table pro_table" style="max-width:100%; margin:0 auto; border:1px solid #ccc;"><thead><tr class="firstRow"><th style="border-right:1px solid #FFF;font-weight:bold;">Detection method</th><th style="border-right:1px solid #FFF;font-weight:bold;">Principles and features</th><th style="font-weight:bold;">Results</th></tr></thead><tbody><tr><td rowspan="2" style="text-align:center;font-weight:bold;">SEC-HPLC</td><td style="text-align: center;font-weight:bold;">Column correction method</td><td rowspan="2" style="text-align:left;">Calculate the relative molecular weight by comparing the elution retention times of the protein to be measured and the standard protein</td></tr><tr><td style="text-align:left;">It is assumed that the conformation of the protein to be measured is similar in shape to the standard protein.<br/>It is assumed that there is no non-specific interaction with the column packing, and each elution time is reproducible without drift</td></tr><tr><td rowspan="2" style="text-align:center;font-weight:bold;">SEC-MALS</td><td style="text-align: center; font-weight:bold;">Light scattering method</td><td rowspan="2" style="text-align:left;">Absolute molecular weight was determined directly by laser scattering intensity. Molecular weight determination was independent of elution volume or retention time.</td></tr><tr><td style="text-align:left;">The intensity of the scattered light is proportional to the square of the molar mass, concentration, and refractive index increment.<br/>The angular dependence of scattering light is only dependent on the size of the molecule.</td></tr></tbody></table></div><div class="layui-row layui-col-space30" id="authors"><div class="layui-col-md8"><div class="cd20ProtocolTitle"><div class="h4-title">Fundamentals of SEC-MALS technology</div></div><div class="public_text_dec">The MALS technique is based on the direct correlation between the molecular weight and the light scattering intensity to determine the absolute molecular weight. There is no need to use a standard protein independent of the elution volume. The components separated by SEC enter the MALS detector. Light scattering occurs when the laser irradiates the analyte. The intensity of the scattered light is measured simultaneously by MALS from multiple angles (Fig. 1.a). Formula b1 shows that the scattering light intensity is proportional to the square of molar mass, concentration, and refractive index increment. Further, the absolute molecular weight of the analyte can be calculated directly by formula b2.</div><div class="public_text_dec">Therefore, in biopharmaceutics, SEC-MALS is widely used in R & D and analytical experiments, characterization of proteins and polymers, evaluation of solution properties, process development, and quality control.</div></div><div class="layui-col-md4"><div class="public_text_dec imgOpen" style="text-align:center !important;"><img src="/static/main/images/sec_mals/sec_mals1.jpg" style="margin-right:10px; margin-top:2px; height:210px; " data-category="Schematic diagram of SEC-MALS detection a. Light scattering sketch b. Molecular weight calculation of analytes" class="facs_img" alt="SEC-MALS, detection diagram" title="SEC-MALS, detection diagram"/><br/><p style="text-align:center;"><em>Fig 1. Schematic diagram of SEC-MALS detection a. Light scattering sketch b. Molecular weight calculation of analytes</em></p></div></div></div><div class="cd20ProtocolTitle"><div class="h4-title">HSPEC-MALS determined the molecular weight of the HIV-1 coated trimer. The reliability of the results was verified by orthogonal mass spectrometry.</div></div><div class="public_text_dec">Researchers of 'Vaccine Production Program' in Laboratory of National Institute of Allergy and Infectious Diseases (NIAID) Vaccine Research Center <sup>[1]</sup> has determined the total molecular weight (339 KDa), the molecular weight of protein (213 KDA), and glycan component (126 KDda) of high glycosylated HIV-1 envelope trimer (Trimer 4571) vaccine by HPSEC-MALS. The assay results were further validated by orthogonal mass spectrometry, which helps to support process development and provide rapid protein characterization data. It demonstrates the broad applicability of HPSEC-MALS to many of the heterologous glycoproteins' candidate vaccines under development.</div><div class="public_img_text_box public_img_l_r layui-col-space10 " style="float: left;width: 100%; text-align:center !important;"><div class="layui-col-md6" style="padding:10px;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals2.jpg" title="Comparison of Trimer 4571 molecular weight results obtained by SEC-MALS with MS data" alt="SEC-MALS compare with MS data" data-category="Comparison of Trimer 4571 molecular weight results obtained by SEC-MALS with MS data" style="max-height:290px !important; height:290px;" class="facs_img"/></div><p class="imgText" style="text-align:center;"><em>Fig. 2. Comparison of Trimer 4571 molecular weight results obtained by SEC-MALS with MS data</em></p></div><div class="layui-col-md6" style="padding:10px;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals3.jpg" style="max-height:290px !important; height:290px;" title="SEC-UV-dRI-MALS" alt="SEC-UV-dRI-MALS" data-category="Determination of Trimer by SEC-UV-dRI-MALS" class="facs_img"/></div><p class="imgText" style="text-align:center;"><em>Fig 3. Determination of Trimer by SEC-UV-dRI-MALS</em></p></div></div><div class="clear"></div><div class="cd20ProtocolTitle"><div class="h4-title">Comparing the results of SEC-HPLC and SEC-MALS with the determination of the molecular weight of commercial drugs</div></div><div class="public_text_dec">Three known antibody drugs, OKT3, Herceptin, and Ipilimumab, were determined by SEC-HPLC and MALS, respectively. As shown in Table 1, the molecular weights of the three antibodies are within a close range of 150-160 kDa. However, the elution retention time of three kinds of antibodies was different, resulting in a molecular weight discrepancy calculated by SEC-HPLC. The OKT3 antibody was 212.5 kDa, and Ipilimumab was only 66.3 kD, which was far from the calculated molecular weight. The molecular weights of the three antibodies detected by SEC-MALS were very close to the theoretical molecular weights (Fig 5).</div><div class="public_text_dec" style="font-weight:bold;">Therefore, MALS technology is far more accurate and reliable than the traditional SEC-HPLC method.</div><div class="public_img_text_box public_img_l_r layui-col-space10 " style="float: left; margin-top:20px;width: 100%; text-align:center !important;"><div class="layui-col-md6" style="padding:10px;"><div class="public_text_dec" style="text-align:center;">Tab 1. Results of molecular weight determination of three antibody drugs<br/><table class="public_table layui-table pro_table pro_table_box"><thead><tr class="firstRow"><th style="width: 20%">Antibody Name</th><th style="width: 20%">Column</th><th style="width: 20%">Retention time<br/>TR (min)</th><th style="width: 20%">Molecular weight <br/>(SEC-HPLC)</th><th style="width: 20%">Molecular weight<br/>(SEC -MALS)</th></tr></thead><tbody><tr><td>OKT3 </td><td>G3000SWxl</td><td>14.775</td><td>212.5 kDa </td><td>161.6 kDa</td></tr><tr><td>Herceptin</td><td>G3000SWxl </td><td>15.436</td><td>146 kDa </td><td>151 kDa </td></tr><tr><td>lpilimumab</td><td>G3000SWxl </td><td>17.039</td><td>66.3 kDa </td><td>172.2 kDa</td></tr></tbody></table></div></div><div class="layui-col-md6" style="padding:10px;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals5.jpg" style="max-height:207px !important; height:207px;" title="TNFSF" alt="TNFSF" data-category="Molecular weight determination of different antibodies by SEC-HPLC" class="facs_img"/></div><p class="imgText" style="text-align:center;"><em>Fig 4. Molecular weight determination of different antibodies by SEC-HPLC</em></p></div></div><div class="layui-col-md12 public_img_text_box" style="padding:10px;text-align:center;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals6.jpg" class="facs_img" style="max-height:274px !important; height:274px;" title="SEC-MALS was used to determine the molecular weight of three antibody drugs" alt="SEC-MALS was used to determine the molecular weight of three antibody drugs" data-category="Determination of Molecular Weight of Three Antibody Drugs by SEC-MALS"/></div><p class="imgText" style="text-align:center;"><em>Fig 5. Determination of Molecular Weight of Three Antibody Drugs by SEC-MALS</em></p></div></div><div class="clear"></div><div class="productSection"><div class="productTit">SEC-MALS: Ensuring the Quality of Polymeric Protein Products</div><div class="public_text_dec">Natural proteins often exist in a certain aggregation state and conformation <span style="font-style:italic">in vivo</span> to ensure that they play a correct biological function. The random and incorrect aggregation of recombinant proteins in the production process often causes changes in the conformation or exposure of certain key functional epitopes. It results in the inability to obtain high-quality antibody candidates targeting effective natural epitopes, which affects the results of in vitro functional verification of antibody drugs and significantly reduces the success rate of antibody-drug development.</div><div class="public_text_dec" style="margin-top:10px;">ACROBiosystems understands the impact of recombinant protein structure on its function and the need for antibody-drug development. Through in-depth study of the structure and properties of the complexes, we designed and developed a variety of homologous/heterologous polymer products using a unique technology platform and verified the molecular weight of the products by SEC-MALS. The SEC-MALS purity results as a quality control standard to ensure more homogeneous product properties smaller inter-batch differences. At the same time, the product activity through ELISA, flow cytometry, functional and other application scenarios validation accelerate the development of antibody drugs.</div><div class="clear"></div><div class="s-con cf"><a class="normal_cf" href="#TNFSF">Case 1: TNFSF Trimer <br/><img src="/static/main/images/sec_mals/sec_mals11_1.png" title="TNFSF" alt="TNFSF" data-category="TNFSF" style="width:300px;text-align:center;"/>           </a> <a class="normal_cf" href="#IL-2R">Case 2: IL-2R Polymer<br/><img src="/static/main/images/sec_mals/sec_mals11_2.png" title="IL-2R polymer" alt="IL-2R polymer" data-category="IL-2R polymer" style="width:300px;text-align:center;"/>          </a> <a class="normal_cf" href="#CTLA-4">Case 3: CTLA-4 Homodimer Proteins <br/><img src="/static/main/images/sec_mals/sec_mals11_3.png" title="CTLA-4 homodimer" alt="CTLA-4 homodimer" data-category="CTLA-4 homodimer" style="width:300px;text-align:center;"/><br/>          </a> <a class="normal_cf" href="#Spike">Case 4: Spike Trimer<br/><img src="/static/main/images/sec_mals/sec_mals11_4.png" title="Spike trimer" alt="Spike trimer" data-category="Spike trimer" style="width:300px;text-align:center;"/><br/>          </a></div><div class="clear"></div><h3 class="h3-title" id="TNFSF">Case 1: TNFSF Trimer</h3><div class="public_text_dec">Tumor necrosis factor (TNF) superfamily proteins are trimeric in nature. Only when the trimeric form of TNF-α binds to the extracellular domain of TNFR1 can it induce trimerization, which effectively stimulates the downstream pathway to induce apoptosis.</div><div class="public_img_text_box public_img_l_r layui-col-space10 " style="float: left;width: 100%;"><div class="layui-col-md6" style="padding:10px;"><div class="cd20ProtocolTitle"><div class="h4-title">Protein structure and purity verified by SEC-MALS</div></div><div class="imgTitle" style="font-weight:bold;">Cat. No. <a href="/P1702-Human-OX40-Ligand--TNFSF4-Protein-His-Tag-%28active-trimer%29-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">OXL-H52Q8</a>(Human OX40 Ligand /TNFSF4 Protein, His Tag, active trimer)</div><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals7.jpg" alt="OX40 Ligand, MALS-validated protein" title="OX40 Ligand, MALS-validated protein" data-category="OX40 Ligand, MALS-validated protein" style="height:210px;" class="facs_img"/></div><p class="imgText"><em>Figure 6. The purity of Human OX40 Ligand, His Tag (active trimer) (Cat. No. o. <a href="/P1702-Human-OX40-Ligand--TNFSF4-Protein-His-Tag-%28active-trimer%29-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">OXL-H52Q8</a> ) is more than 95% in HP-SEC, and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.</em></p></div><div class="layui-col-md6" style="padding:10px;"><div class="cd20ProtocolTitle"><div class="h4-title">Cell functional activity verified by FACS</div></div><div class="imgTitle" style="font-weight:bold;">Cat. No. <a href="/P1702-Human-OX40-Ligand--TNFSF4-Protein-His-Tag-%28active-trimer%29-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">OXL-H52Q8</a>(Human OX40 Ligand /TNFSF4 Protein, His Tag, active trimer)</div><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals8.jpg" alt="OX40 Ligand, FACS-validated protein" title="OX40 Ligand, FACS-validated protein" data-category="OX40 Ligand, FACS-validated protein" style="height:210px;" class="facs_img"/></div><p class="imgText" id="IL-2R"><em>Figure 7. FACS analysis shows that human sources OX40 Ligand, His Tag (active trimer) (Cat. No. <a href="/P1702-Human-OX40-Ligand--TNFSF4-Protein-His-Tag-%28active-trimer%29-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">OXL-H52Q8</a> ) can bind to 293T cells overexpressing human OX40. The concentration of OX40 Ligand was 0.1 μg / mL. </em></p><center><label class="btn_protocol des_protocol"><a style="color:#FFFFFF" data-sign="OXL-H52Q8">Protocol</a></label></center></div></div><div class="clear"></div><h3 class="h3-title">Case 2: IL-2R Heterodimer / Trimer</h3><div class="public_text_dec">The receptor for IL-2 (IL-2R) contains three subunits of α, β, and γ. Three subunits can form receptors with different affinities. In the absence of IL-2, α, β, and γ, a specific structure of the IL-2 receptor complex cannot directly form a high-affinity IL-2 receptor complex. ACROBiosystems has developed a range of proprietary technologies IL-2 R alpha & IL-2 R beta & IL-2 R gamma Heterotrimer protein and IL-2 R beta & IL-2 R gamma Heterodimer protein.</div><div class="cd20ProtocolTitle"><div class="h4-title">Protein structure and purity verified by SEC-MALS</div></div><div class="public_img_text_box public_img_l_r layui-col-space10 " style="float: left;width: 100%;"><div class="layui-col-md6" style="padding:10px; float:left;"><div class="imgTitle" style="font-weight:bold;">Cat. No. <a href="/P3276-Human-IL-2-R-betaIL-2-R-gamma-Heterodimer-Protein-Fc-TagFc-Tag-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">ILG-H5254</a>(Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag)</div><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals9.jpg" title="IL-2 R beta & IL-2 R gamma, MALS-validated protein" data-category="IL-2 R beta & IL-2 R gamma, MALS-validated protein" alt="IL-2 R beta & IL-2 R gamma, MALS-validated protein" style="max-height:207px !important; height:207px;" class="facs_img"/></div><p class="imgText"><em>Fig. 8. The purity of Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. <a href="/P3276-Human-IL-2-R-betaIL-2-R-gamma-Heterodimer-Protein-Fc-TagFc-Tag-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">ILG-H5254</a> )is more than 90% and the molecular weight of this protein is around 1 145 ~ 165 kDa verified by SEC-MALS.</em></p></div><div class="layui-col-md6" style="padding:10px; float:right;"><div class="imgTitle" style="font-weight:bold;">Cat. No. <a href="/P3494-Human-IL-2-R-betaIL-2-R-alphaIL-2-R-gamma-Protein-Fc-TagFc-Tag-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">ILG-H5257</a>(Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag)</div><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals10.jpg" data-category="IL-2 R beta & IL-2 R alpha & IL-2 R gamma, MALS-validated protein" alt="IL-2 R beta & IL-2 R alpha & IL-2 R gamma, MALS-validated protein" title="IL-2 R beta & IL-2 R alpha & IL-2 R gamma, MALS-validated protein" style="max-height:207px !important; height:207px;" class="facs_img"/></div><p class="imgText"><em>Fig 9. The purity of Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. <a href="/P3494-Human-IL-2-R-betaIL-2-R-alphaIL-2-R-gamma-Protein-Fc-TagFc-Tag-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">ILG-H5257</a> ) is more than 90% and the molecular weight of this protein is around 175-190 kDa verified by SEC-MALS.</em></p></div></div><div class="cd20ProtocolTitle"><div class="h4-title">After SPR verification, the affinity verification results of IL-2 and IL-2 receptors are consistent with the literature <sup>[2]</sup> .</div></div><div class="layui-col-md12 public_img_text_box" style="text-align: center !important;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals11.jpg" data-category="IL-2 and IL-2 receptors affinity verified by SPR" alt="IL-2 and IL-2 receptors affinity verified by SPR" title="IL-2 and IL-2 receptors affinity verified by SPR" style="height:340px;max-height:340px !important; " class="facs_img"/></div><p class="imgText" id="CTLA-4" style="text-align:center;"><em>Fig 10. SPR verified the binding affinity of IL-2 to ACRO's IL-2R construct.</em></p></div><div class="clear"></div><h3 class="h3-title">Case 3: CTLA-4 Homodimer Proteins</h3><div class="public_text_dec">With the rise of bispecific drugs, there is a wave of research and development of dual-targeted based on PD-1 / PD-L1 and CTLA-4. At present, there are many similar bispecific drugs in the clinical validation stage, and its indications also cover a variety of solid tumors. CTLA-4 (cytotoxic T lymphocyte-associated protein 4), also called CD152 (differentiation cluster 152), is a protein receptor of T cells, usually in the form of a homodimer.</div><div class="cd20ProtocolTitle"><div class="h4-title">Protein structure and purity verified by SEC-MALS</div></div><div class="imgTitle" style="font-weight:bold;">Cat. No. <a href="/P3462-Human-CTLA-4--CD152-Protein-His-Tag-active-dimer-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">CT4-H52H9</a>(Human CTLA-4 / CD152 Protein, His Tag, active dimer (MALS verified))</div><div class="public_img_text_box public_img_l_r layui-col-space10 " style="float: left;width: 100%;"><div class="layui-col-md12 public_img_text_box" style="padding:10px;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals12.jpg" data-category="CTLA-4, MALS-validated protein" title="CTLA-4, MALS-validated protein" alt="CTLA-4, MALS-validated protein" style="max-height:240px !important; height:240px;" class="facs_img"/></div><p class="imgText" style="text-align:center;"><em>Fig 11. The purity of Human CTLA-4, His Tag (Cat. No. <a href="/P3462-Human-CTLA-4--CD152-Protein-His-Tag-active-dimer-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">CT4-H52H9</a> ) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.</em></p></div><div class="cd20ProtocolTitle"><div class="h4-title">CTLA-4 dimer affinity verified by SPR</div></div><div class="layui-col-md12 public_img_text_box" style="padding:10px;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals13.jpg" data-category="CTLA-4 dimer affinity verified by SPR" title="CTLA-4 dimer affinity verified by SPR" alt="CTLA-4 dimer affinity verified by SPR" style="max-height:240px !important; height:240px;" class="facs_img"/></div><p class="imgText" id="Spike" style="text-align:center;"><em>Fig 12. SPR verified that the affinity of CTLA-4 dimer is more than ten times higher than that of monomer</em></p></div></div><div class="clear"></div><h3 class="h3-title">Case 4: Spike Trimer</h3><div class="public_text_dec">The S protein is the most important target of coronavirus research. The SARS-CoV-2 virus S-protein functions as a trimer; it consists of three identical molecules encoded by the same gene. Each of these molecules has two subunits, namely S1 and S2. When the RBD in the S1 subunit binds to the host cell ACE2, it causes trimer instability. The S1 and S2 subunit dissociated, and the S2 sub-unit conformation changed into a highly stable post-fusion structure. The S protein has two conformations: prefusion and post-fusion. The researchers found that the conformational form of the S protein is unstable, even if it does not bind to the host cell receptor ACE2. There are also spontaneous conformational changes from Prefusion to Postfusion, which pose great challenges for vaccine development and neutralizing antibody screening.</div><div class="cd20ProtocolTitle"><div class="h4-title">Protein structure and purity verified by SEC-MALS & NS-EM</div></div><p class="imgText" style="font-weight:bold;">Cat. No. <a href="/P3349-SARS-CoV-2-S-protein-His-Tag-Super-stable-trimer-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">SPN-C52H9</a>(SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified))</p><div class="layui-col-md12 public_img_text_box" style="text-align: center !important;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals14.jpg" title="SARS-CoV-2 spike trimer, SEC-MALS& NS-EM validated protein" alt="SARS-CoV-2 spike trimer, SEC-MALS& NS-EM validated protein" data-category="SARS-CoV-2 spike trimer, SEC-MALS& NS-EM validated protein" style="height:280px;max-height:280px !important;" class="facs_img"/></div><p class="imgText"><em>Fig 13. SDS-PAGE identified the SARS-CoV-2 S protein under reduced conditions. His Tag, Super stable trimer (MALS & NS-EM verified) (Cat. No. <a href="/P3349-SARS-CoV-2-S-protein-His-Tag-Super-stable-trimer-%28MALS-verified%29.html" target="_blank" style="color:#cc3536; text-decoration:underline;">SPN-C52H9</a> ) The purity is over 90%, and the molecular weight is about 550-660 kDa by SEC-MALS. The size and appearance of the SARS-CoV-2 trimer verified with NS-EM were like those reported in the published literature.</em></p></div><div class="cd20ProtocolTitle"><div class="h4-title">S protein trimer can be used to detect antibody titers in serum of rehabilitated patients with a high signal-to-noise ratio</div></div><div class="clear"></div><div class="layui-col-md12 public_img_text_box" style="text-align: center !important;"><div class="imgBox imgOpen"><img src="/static/main/images/sec_mals/sec_mals15.jpg" title="SARS-CoV-2 S protein shows high signal-to-noise ratio" alt="SARS-CoV-2 S protein shows high signal-to-noise ratio" data-category="SARS-CoV-2 S protein shows high signal-to-noise ratio" style="height:360px;max-height:360px !important;" class="facs_img"/></div><p class="imgText"><em>Fig 14. Comparative analysis of antibody titers of serum samples from rehabilitated patients detected by S-6P protein, S-2P protein, and S protein. The results showed that the signal-to-noise ratio of S-6P was significantly higher than that of S protein, S-2P, and other S proteins. It suggested that S-6P was more suitable for antibody titer detection in serum samples and could detect antibody titers in serum early after injection of vaccine, which was helpful for evaluation of vaccine immunogenicity.</em></p></div></div><div class="clear"></div><div class="productSection"><div class="productTit">More MALS-validated Polymer Complexes</div><div class="public_img_text_box layui-col-space12 "><div class="layui-col-md12"><div class="product-type layui-row"><div class="s-con cf"><a href="/P4010-Human-CD3-epsilonCD3-delta-Heterodimer-Protein-FcHis-TagFcFlag-Tag-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>CD3E&CD3D<br/><span style="font-size:0.9em;">(Star products: Cat. No. CDD-H52Wa)</span> </strong></p></a> <a href="/P4265-Human-LRRC32TGF-beta-1-Heterotrimer-protein-His-TagTag-Free-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>LRRC32&TGF beta-1<br/><span style="font-size:0.9em;">(Star products: Cat. No.GA1-H52W9)</span> </strong></p></a> <a href="/P4198-Sus-scrofa-CD73--NT5E-Protein-His-Tag-%28active-enzyme-MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>CD73 <br/><span style="font-size:0.9em;">(Star products: Cat. No.CD3-S52H3) </span> </strong></p></a><a href="/P3240-Human-FAP-Protein-His-Tag-active-dimer-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>FAP protein <br/><span style="font-size:0.9em;">(Star products: Cat. No.FAP-H5244) </span> </strong></p></a><a href="/P2784-Human-PSMA--FOLH1-Protein-His-Tag-%28active-enzyme-MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>PSMA <br/><span style="font-size:0.9em;">(Star products: Cat. No.PSA-H52H3)</span></strong></p></a>    <a href="/P4133-Human-TIGIT-Protein-His-Tag-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>TIGIT <br/><span style="font-size:0.9em;">(Star products: Cat. No.TIT-H52H5) </span> </strong></p></a> <a href="/P3672-Human-CD79ACD79B-Heterodimer-Protein-Mouse-IgG2a-FcFlag-TagMouse-IgG2a-FcHis-Tag-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>CD79A&CD79B <br/><span style="font-size:0.9em;">(Star products: Cat. No.CDB-H52W3) </span></strong></p></a> <a href="/P3882-Human-IL-17AIL-17F-Heterodimer-Protein-Twin-StrepHis-Tag-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>IL-17A & IL-17F <br/><span style="font-size:0.9em;">(Star products: Cat. No.ILF-H52W6) </span></strong></p></a><a href="/P3957-Human-IL-23-alpha--Rat-IL-12-beta-Heterodimer-Protein-His-TagTag-Free-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>IL23A & IL12B<br/><span style="font-size:0.9em;">(Star products: Cat. No.ILB-HR52W3) </span> </strong></p></a> <a href="/P3834-Mouse-Integrin-alpha-2-beta-1-%28ITGA2ITGB1%29-Heterodimer-Protein-His-TagTag-Free-%28MALS-verified%29.html" class="normal_cf" target="_blank"><p><strong>Integrin alpha 2 beta 1<br/><span style="font-size:0.9em;">(Star products: Cat. No.IT1-M52W3) </span> </strong></p></a></div></div></div></div></div><div class="clear"></div><div class="clear"></div><div class="productSection"><div class="productTit">Product List</div><div class="layui-tab layui-tab-card" lay-filter="module"><ul class="layui-tab-title list-paddingleft-2"><li class="layui-this" lay-id="product1" id="product1"><p>TNFSF</p></li><li lay-id="product2" id="product2"><p>CD3</p></li><li lay-id="product3" id="product3"><p>FcRn (FCGRT & B2M)</p></li><li lay-id="product4" id="product4"><p>Interleukin</p></li><li lay-id="product5" id="product5"><p>Spike trimer</p></li><li lay-id="product6" id="product6"><p>Others</p></li></ul><div class="layui-tab-content"><div class="layui-tab-item layui-show"><table class="public_table layui-table pro_table pro_table_box"><thead><tr class="firstRow"><th style="width: 15%">Molecule</th><th style="width: 15%">Cat. No.</th><th>Host</th><th class="pro_desc">Product Description</th><th style="width: 8%">Structure</th></tr></thead><tbody id="product_list_809" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_919" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_32" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_114" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_97" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_213" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_896" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_506" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_497" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_856" style="border: 1px solid #ccc;border-top:none"></tbody></table></div><div class="layui-tab-item"><table class="public_table layui-table pro_table"><thead><tr class="firstRow"><th style="width: 15%">Molecule</th><th style="width: 15%">Cat. No.</th><th>Host</th><th class="pro_desc">Product Description</th><th style="width: 8%">Structure</th></tr></thead><tbody id="product_list_94" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_823" style="border: 1px solid #ccc;border-top:none"></tbody></table></div><div class="layui-tab-item"><table class="public_table layui-table pro_table"><thead><tr class="firstRow"><th style="width: 15%">Molecule</th><th style="width: 15%">Cat. No.</th><th>Host</th><th class="pro_desc">Product Description</th><th style="width: 8%">Structure</th></tr></thead><tbody id="product_list_216" style="border: 1px solid #ccc;border-top:none"></tbody></table></div><div class="layui-tab-item"><table class="public_table layui-table pro_table"><thead><tr class="firstRow"><th style="width: 15%">Molecule</th><th style="width: 15%">Cat. No.</th><th>Host</th><th class="pro_desc">Product Description</th><th style="width: 8%">Structure</th></tr></thead><tbody id="product_list_849" style="border: 2px solid #ccc;border-top:none"></tbody><tbody id="product_list_866" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1062" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1114" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_821" style="border: 1px solid #ccc;border-top:none"></tbody></table></div><div class="layui-tab-item"><table class="public_table layui-table pro_table"><thead><tr class="firstRow"><th style="width: 15%">Molecule</th><th style="width: 15%">Cat. No.</th><th>Host</th><th class="pro_desc">Product Description</th><th style="width: 8%">Structure</th></tr></thead><tbody id="product_list_1145" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1122" style="border: 1px solid #ccc;border-top:none"></tbody></table></div><div class="layui-tab-item"><table class="public_table layui-table pro_table"><tbody><tr class="firstRow"><th style="width: 15%">Molecule</th><th style="width: 15%">Cat. No.</th><th>Host</th><th class="pro_desc">Product Description</th><th style="width: 8%">Structure</th></tr></tbody><tbody id="product_list_78" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1126" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1047" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_161" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_182" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1055" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_937" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_1191" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_229" style="border: 1px solid #ccc;border-top:none"></tbody><tbody id="product_list_491" style="border: 1px solid #ccc;border-top:none"></tbody></table></div></div></div></div><div class="clear"></div><div class="clear"></div><h3 class="public_h3_title">References</h3><ul class="referList list-paddingleft-2"><li><p>[1]Bender M F , Li Y , Ivleva V B , et al. Protein and glycan molecular weight determination of highly glycosylated HIV-1 envelope trimers by HPSEC-MALS[J]. Vaccine, 2021(7523).</p></li><li><p>[2]Sriram B , Tania C R ,  Davis A M , et al. Ligand binding kinetics of IL-2 and IL-15 to heteromers formed by extracellular domains of the three IL-2 receptor subunits[J]. International Immunology(11):1839.</p></li></ul></div></div><style>.s-con{margin: 10px 0px; } .s-con .normal_cf{display:block;margin-left: 0.4%;margin-top: 0.4%;float: left;width:23.8%; color:#333; background-color: #f2f2f2;text-align: center;font-size: 14px;padding:10px 5px; line-height:26px; position:relative; min-height:55px; font-weight:bold; } .s-con .normal_cf1{width:100%;margin-top:10px;} .s-con a:hover{ color: #cc3536;background-color:#ffdcda;text-decoration: underline; } .s-cona{margin: 10px 0px; } .s-cona a{display:block;margin-left: 0.4%;margin-top: 0.4%;float: left;width:18.8%; color:#333; background-color: #f2f2f2;text-align: center;font-size: 14px;padding:10px 5px; line-height:26px; position:relative; min-height:55px; font-weight:bold; } .s-cona a:hover{ color: #fff;background-color:#cc3536;text-decoration: underline; } #authors{ margin-top: 20px; margin-bottom:20px; border-width: 1px; border-top-width: 1px; border-top-width: 1px; border-style: solid; border-top-style: 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comdiv.style.height = "498px"; sidebar.style.width = "318px"; sidebar.style.height = "498px"; clearInterval(loadInit2); }; } else { clearInterval(loadInit2); } }; } if(minbutton2 != undefined){ minbutton2.onclick = function () { dismaxbutton.style.display= 'block'; disminbutton.style.display= 'none'; } } if(closebutton != undefined){ closebutton.onclick = function () { dismaxbutton.style.display= 'block'; disminbutton.style.display= 'none'; } } loadInput(); // 弹窗移动 if(buttonElement != undefined){ buttonElement.onmousedown = function (e) { //spaceX鼠标在登录弹窗中，距离左边框的左偏移量 var spaceX = e.clientX - comdiv.offsetLeft; //spaceY鼠标在登录弹窗中，距离上边框的上偏移量 var spaceY = e.clientY - comdiv.offsetTop; //按下login_title区域，为整个网页文档注册鼠标移动事件 document.onmousemove = function (e) { if(comdiv.style.width == "320px"){ sidebar.style.width = "318px" } var clientHeight = document.documentElement.clientHeight || document.body.clientHeight; var clientwidth = document.documentElement.clientWidth || document.body.clientWidth; var x = e.clientX - spaceX; // 移动弹窗时左右限制 if(x <0){ x=0; } else if(x > clientwidth - comdiv.offsetWidth){ x = clientwidth - comdiv.offsetWidth; } // 移动弹窗时上下限制 var y = e.clientY - spaceY; if(y <0){ y=0; } else if(y >clientHeight -comdiv.offsetHeight){ y = clientHeight -comdiv.offsetHeight; } comdiv.style.left = x + "px"; comdiv.style.top = y + "px"; }; e.preventDefault(); }; //松开下login_title区域，为整个网页文档解绑鼠标移动事件 buttonElement.onmouseup = function (evn) { evn.preventDefault(); buttonElement.onmousemove = null; document.onmousemove = null; }; } } if(comdiv != undefined){ // 定义4个变量 var disX = 0;//鼠标按下时光标的X值 var disY = 0;//鼠标按下时光标的Y值 var disW = 0; //拖拽前div的宽 var disH = 0; // 拖拽前div的高 //3. 给小div加点击事件 comdiv.onmouseover = function (evs) { var evs = evs || window.event; var offsetLeft = comdiv.offsetLeft; var getfoc = Math.abs(evs.clientX - (parseInt(offsetLeft)+comdiv.offsetWidth)); console.log('getfoc====>'+getfoc); if( getfoc == 1 || getfoc == 2 ){ comdiv.style.cursor ='col-resize'; document.onmousedown = function (ev) { var ev = ev || window.event; disX = ev.clientX; // 获取鼠标按下时光标x的值 disY = ev.clientY; // 获取鼠标按下时光标Y的值 if(sidebar != undefined){ disW = sidebar.offsetWidth; // 获取拖拽前div的宽 disH = sidebar.offsetHeight; // 获取拖拽前div的高 } document.onmousemove = function (evm) { var clientHeight = document.documentElement.clientHeight || document.body.clientHeight; var clientwidth = document.documentElement.clientWidth || document.body.clientWidth; console.log('clientwidth===>'+clientwidth); var evm = evm || window.event; //拖拽时为了对宽和高限制一下范围，定义两个变量 var W = evm.clientX - disX + disW; var H = evm.clientY - disY + disH; if (W < 320) { W = 320; } else if (W > clientwidth-40 ){ W = clientwidth-40 ; } if (H < 498) { H = 498; } else if (H > clientHeight ){ H = clientHeight ; } if(sidebar != undefined){ sidebar.style.width = (W-2) + 'px';// 拖拽后物体的宽 sidebar.style.height = H + 'px';// 拖拽后物体的高 } if(comdiv != undefined){ comdiv.style.width = W + 'px';// 拖拽后物体的宽 comdiv.style.height = H + 'px';// 拖拽后物体的高 } evm.preventDefault(); } document.onmouseup = function (evn) { var evn = evn || window.event; 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