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class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Pancreatic cancer is a rapidly fatal disease, and there is an urgent need for early detection markers and novel therapeutic targets. The current study has used a proteomic approach of two-dimensional (2D) gel electrophoresis and mass... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_73963520" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Pancreatic cancer is a rapidly fatal disease, and there is an urgent need for early detection markers and novel therapeutic targets. The current study has used a proteomic approach of two-dimensional (2D) gel electrophoresis and mass spectrometry (MS) to identify differentially expressed ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/73963520" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ebb4ff69753cdc8a79287cde2df714ef" rel="nofollow" data-download="{"attachment_id":82289819,"asset_id":73963520,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/82289819/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4624315" href="https://independent.academia.edu/MariaPerson">Maria Person</a><script data-card-contents-for-user="4624315" type="text/json">{"id":4624315,"first_name":"Maria","last_name":"Person","domain_name":"independent","page_name":"MariaPerson","display_name":"Maria Person","profile_url":"https://independent.academia.edu/MariaPerson?f_ri=5769","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_73963520 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="73963520"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 73963520, container: ".js-paper-rank-work_73963520", }); 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The current study has used a proteomic approach of two-dimensional (2D) gel electrophoresis and mass spectrometry (MS) to identify differentially expressed ...","downloadable_attachments":[{"id":82289819,"asset_id":73963520,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4624315,"first_name":"Maria","last_name":"Person","domain_name":"independent","page_name":"MariaPerson","display_name":"Maria Person","profile_url":"https://independent.academia.edu/MariaPerson?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5769,"name":"Mass 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Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=5769"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=5769"},{"id":80692,"name":"Copper","url":"https://www.academia.edu/Documents/in/Copper?f_ri=5769"},{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease?f_ri=5769"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=5769"},{"id":295257,"name":"Antioxidant enzyme","url":"https://www.academia.edu/Documents/in/Antioxidant_enzyme?f_ri=5769"},{"id":371528,"name":"Early Detection","url":"https://www.academia.edu/Documents/in/Early_Detection?f_ri=5769"},{"id":501806,"name":"Protein Expression","url":"https://www.academia.edu/Documents/in/Protein_Expression?f_ri=5769"},{"id":1010730,"name":"Calcium Binding Protein","url":"https://www.academia.edu/Documents/in/Calcium_Binding_Protein?f_ri=5769"},{"id":1217494,"name":"Early Diagnosis","url":"https://www.academia.edu/Documents/in/Early_Diagnosis?f_ri=5769"},{"id":1817281,"name":"Extracellular Matrix Proteins","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix_Proteins?f_ri=5769"},{"id":2280042,"name":"Differential expression","url":"https://www.academia.edu/Documents/in/Differential_expression?f_ri=5769"},{"id":3521397,"name":"Pancreatic neoplasms","url":"https://www.academia.edu/Documents/in/Pancreatic_neoplasms?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_66306183" data-work_id="66306183" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66306183/Environmental_applications_of_thermal_extraction_cone_penetrometry_and_ultrafast_gas_chromatography_mass_spectrometry">Environmental applications of thermal extraction cone penetrometry and ultrafast gas chromatography/mass spectrometry</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66306183" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="68d458f9dcec31c0f206f1498c77b508" rel="nofollow" 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Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="100842" href="https://www.academia.edu/Documents/in/Electronic_Nose">Electronic Nose</a>, <script data-card-contents-for-ri="100842" type="text/json">{"id":100842,"name":"Electronic Nose","url":"https://www.academia.edu/Documents/in/Electronic_Nose?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="184001" href="https://www.academia.edu/Documents/in/Polycyclic_Aromatic_Hydrocarbon">Polycyclic Aromatic Hydrocarbon</a><script data-card-contents-for-ri="184001" type="text/json">{"id":184001,"name":"Polycyclic Aromatic Hydrocarbon","url":"https://www.academia.edu/Documents/in/Polycyclic_Aromatic_Hydrocarbon?f_ri=5769","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=66306183]'), work: {"id":66306183,"title":"Environmental applications of thermal extraction cone penetrometry and ultrafast gas chromatography/mass spectrometry","created_at":"2021-12-28T23:02:14.370-08:00","url":"https://www.academia.edu/66306183/Environmental_applications_of_thermal_extraction_cone_penetrometry_and_ultrafast_gas_chromatography_mass_spectrometry?f_ri=5769","dom_id":"work_66306183","summary":null,"downloadable_attachments":[{"id":77551904,"asset_id":66306183,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35681884,"first_name":"Albert","last_name":"Robbat","domain_name":"tufts","page_name":"AlbertRobbat","display_name":"Albert Robbat","profile_url":"https://tufts.academia.edu/AlbertRobbat?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass 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js-work-card work_63432969" data-work_id="63432969" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/63432969/Experimental_Determination_of_the_Translational_Acceleration_Values_for_a_Spinning_Vehicle">Experimental Determination of the Translational Acceleration Values for a Spinning Vehicle</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">reconstructionists often determine pre-collision conditions based on post-collision evidence. Energy-based speed calculations for skidding vehicles are commonly used and have been the basis for numerous theoretical and experimental works.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_63432969" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">reconstructionists often determine pre-collision conditions based on post-collision evidence. Energy-based speed calculations for skidding vehicles are commonly used and have been the basis for numerous theoretical and experimental works. The simplest analysis technique, &quot;skid-to-stop&quot; is fairly well understood, however the more complex circumstance of a spinning trajectory is less well understood. This paper will describe a series of experiments conducted to evaluate the frictional values applicable to a spinning vehicle as it rotates and translates without brake application.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/63432969" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="86a8dd1ec029fdcadb2cc7dcaf621e85" rel="nofollow" data-download="{"attachment_id":75864756,"asset_id":63432969,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75864756/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="146076119" href="https://independent.academia.edu/WadeBartlett1">Wade Bartlett</a><script data-card-contents-for-user="146076119" type="text/json">{"id":146076119,"first_name":"Wade","last_name":"Bartlett","domain_name":"independent","page_name":"WadeBartlett1","display_name":"Wade Bartlett","profile_url":"https://independent.academia.edu/WadeBartlett1?f_ri=5769","photo":"https://0.academia-photos.com/146076119/46265385/38480468/s65_wade.bartlett.jpg"}</script></span></span></li><li class="js-paper-rank-work_63432969 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="63432969"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 63432969, container: ".js-paper-rank-work_63432969", }); 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Energy-based speed calculations for skidding vehicles are commonly used and have been the basis for numerous theoretical and experimental works. The simplest analysis technique, \u0026quot;skid-to-stop\u0026quot; is fairly well understood, however the more complex circumstance of a spinning trajectory is less well understood. This paper will describe a series of experiments conducted to evaluate the frictional values applicable to a spinning vehicle as it rotates and translates without brake application.","downloadable_attachments":[{"id":75864756,"asset_id":63432969,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":146076119,"first_name":"Wade","last_name":"Bartlett","domain_name":"independent","page_name":"WadeBartlett1","display_name":"Wade Bartlett","profile_url":"https://independent.academia.edu/WadeBartlett1?f_ri=5769","photo":"https://0.academia-photos.com/146076119/46265385/38480468/s65_wade.bartlett.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=5769","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=5769","nofollow":false},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=5769"},{"id":34019,"name":"Monte Carlo Methods","url":"https://www.academia.edu/Documents/in/Monte_Carlo_Methods?f_ri=5769"},{"id":63353,"name":"Identification","url":"https://www.academia.edu/Documents/in/Identification?f_ri=5769"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=5769"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=5769"},{"id":159446,"name":"Expression","url":"https://www.academia.edu/Documents/in/Expression?f_ri=5769"},{"id":413295,"name":"Kinetic Energy","url":"https://www.academia.edu/Documents/in/Kinetic_Energy?f_ri=5769"},{"id":796887,"name":"Accident Reconstruction","url":"https://www.academia.edu/Documents/in/Accident_Reconstruction?f_ri=5769"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=5769"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=5769"},{"id":865697,"name":"In Silico","url":"https://www.academia.edu/Documents/in/In_Silico?f_ri=5769"},{"id":1009208,"name":"Centre of Mass","url":"https://www.academia.edu/Documents/in/Centre_of_Mass?f_ri=5769"},{"id":1159038,"name":"Epitopes","url":"https://www.academia.edu/Documents/in/Epitopes?f_ri=5769"},{"id":1186610,"name":"DNA binding proteins","url":"https://www.academia.edu/Documents/in/DNA_binding_proteins?f_ri=5769"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=5769"},{"id":3458391,"name":"frameshift mutation","url":"https://www.academia.edu/Documents/in/frameshift_mutation?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_61735079" data-work_id="61735079" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/61735079/Formation_of_N_Ethylmaleimide_NEM_Glutathione_Conjugate_and_N_Ethylmaleamic_Acid_Revealed_by_Mass_Spectral_Characterization_of_Intracellular_and_Extracellular_">Formation of N-Ethylmaleimide (NEM)-Glutathione Conjugate and N-Ethylmaleamic Acid Revealed by Mass Spectral Characterization of Intracellular and Extracellular …</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The extracellular and intracellular metabolites formed upon exposure of activated sludge microorganisms to a sublethal concentration of N-ethylmaleimide were monitored by liquid chromatography with ion trap mass spectrometry. The... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_61735079" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The extracellular and intracellular metabolites formed upon exposure of activated sludge microorganisms to a sublethal concentration of N-ethylmaleimide were monitored by liquid chromatography with ion trap mass spectrometry. The metabolite N-ethylsuccinimido-S-glutathione (m/z 433) was converted rapidly to N-(2-oxoethyl)-2,2-(propionylamino)propanamide (m/z 187) and N-ethylmaleamic acid (m/z 144).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/61735079" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="23309edb89d4894cc47ded2b179572f7" rel="nofollow" data-download="{"attachment_id":74697822,"asset_id":61735079,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74697822/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="17131321" href="https://independent.academia.edu/ElmerRicoMojica">Elmer-Rico Mojica</a><script data-card-contents-for-user="17131321" type="text/json">{"id":17131321,"first_name":"Elmer-Rico","last_name":"Mojica","domain_name":"independent","page_name":"ElmerRicoMojica","display_name":"Elmer-Rico Mojica","profile_url":"https://independent.academia.edu/ElmerRicoMojica?f_ri=5769","photo":"https://0.academia-photos.com/17131321/4717787/5442063/s65_elmer-rico.mojica.jpg"}</script></span></span></li><li class="js-paper-rank-work_61735079 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="61735079"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 61735079, container: ".js-paper-rank-work_61735079", }); 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The metabolite N-ethylsuccinimido-S-glutathione (m/z 433) was converted rapidly to N-(2-oxoethyl)-2,2-(propionylamino)propanamide (m/z 187) and N-ethylmaleamic acid (m/z 144).","downloadable_attachments":[{"id":74697822,"asset_id":61735079,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":17131321,"first_name":"Elmer-Rico","last_name":"Mojica","domain_name":"independent","page_name":"ElmerRicoMojica","display_name":"Elmer-Rico Mojica","profile_url":"https://independent.academia.edu/ElmerRicoMojica?f_ri=5769","photo":"https://0.academia-photos.com/17131321/4717787/5442063/s65_elmer-rico.mojica.jpg"}],"research_interests":[{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":16122,"name":"Activated 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window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_42085733"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_42085733 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="42085733"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 42085733; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=42085733]").text(description); $(".js-view-count-work_42085733").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_42085733").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="42085733"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="923" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5769" href="https://www.academia.edu/Documents/in/Mass_Spectrometry">Mass Spectrometry</a>, <script data-card-contents-for-ri="5769" type="text/json">{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39752" href="https://www.academia.edu/Documents/in/Adsorption">Adsorption</a>, <script data-card-contents-for-ri="39752" type="text/json">{"id":39752,"name":"Adsorption","url":"https://www.academia.edu/Documents/in/Adsorption?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=5769","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=42085733]'), work: {"id":42085733,"title":"Nucleic Acids in Protein Samples Interfere with Phosphopeptide Identification by Immobilized-Metal-Ion Affinity Chromatography and Mass Spectrometry","created_at":"2020-02-27T06:01:07.587-08:00","url":"https://www.academia.edu/42085733/Nucleic_Acids_in_Protein_Samples_Interfere_with_Phosphopeptide_Identification_by_Immobilized_Metal_Ion_Affinity_Chromatography_and_Mass_Spectrometry?f_ri=5769","dom_id":"work_42085733","summary":null,"downloadable_attachments":[{"id":62214974,"asset_id":42085733,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":99388515,"first_name":"YOUHE","last_name":"GAO","domain_name":"independent","page_name":"YOUHEGAO","display_name":"YOUHE GAO","profile_url":"https://independent.academia.edu/YOUHEGAO?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":39752,"name":"Adsorption","url":"https://www.academia.edu/Documents/in/Adsorption?f_ri=5769","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=5769","nofollow":false},{"id":63353,"name":"Identification","url":"https://www.academia.edu/Documents/in/Identification?f_ri=5769"},{"id":158681,"name":"Molecular Biotechnology","url":"https://www.academia.edu/Documents/in/Molecular_Biotechnology?f_ri=5769"},{"id":172678,"name":"Artifacts","url":"https://www.academia.edu/Documents/in/Artifacts?f_ri=5769"},{"id":178906,"name":"Nucleic Acids","url":"https://www.academia.edu/Documents/in/Nucleic_Acids?f_ri=5769"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=5769"},{"id":201241,"name":"Affinity chromatography","url":"https://www.academia.edu/Documents/in/Affinity_chromatography?f_ri=5769"},{"id":223715,"name":"Sample","url":"https://www.academia.edu/Documents/in/Sample?f_ri=5769"},{"id":255058,"name":"Metals","url":"https://www.academia.edu/Documents/in/Metals?f_ri=5769"},{"id":291658,"name":"Precipitation","url":"https://www.academia.edu/Documents/in/Precipitation?f_ri=5769"},{"id":1627605,"name":"Phosphopeptides","url":"https://www.academia.edu/Documents/in/Phosphopeptides?f_ri=5769"},{"id":1727314,"name":"Nucleic Acid","url":"https://www.academia.edu/Documents/in/Nucleic_Acid?f_ri=5769"},{"id":1939264,"name":"single stranded DNA","url":"https://www.academia.edu/Documents/in/single_stranded_DNA?f_ri=5769"},{"id":3544702,"name":"Complex Mixtures","url":"https://www.academia.edu/Documents/in/Complex_Mixtures?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3456141" data-work_id="3456141" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3456141/Electrothermal_vaporization_coupled_with_inductively_coupled_plasma_array_detector_mass_spectrometry_for_the_multielement_analysis_of_Al_2O_3_ceramic_powders">Electrothermal vaporization coupled with inductively coupled plasma array–detector mass spectrometry for the multielement analysis of Al 2O 3 ceramic powders</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">An electrothermal vaporization (ETV) system useful for the analysis of solutions and slurries has been coupled with a sector-field inductively coupled plasma mass spectrometer (ICP–MS) equipped with an array detector. The ability of this... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3456141" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">An electrothermal vaporization (ETV) system useful for the analysis of solutions and slurries has been coupled with a sector-field inductively coupled plasma mass spectrometer (ICP–MS) equipped with an array detector. The ability of this instrument to record the transient signals produced for a number of analytes in ETV–ICP–MS is demonstrated. Detection limits for Mn, Fe, Co, Ni, Cu, Zn and Ga are in the range of 4–60 pg μL− 1 for aqueous solutions and in the low μg g− 1 range for the analysis of 10 mg mL− 1 slurries of Al2O3 powders. The dynamic ranges measured for Fe, Cu and Ga spanned 3–5 orders of magnitude when the detector was operated in the low-gain mode and appear to be limited by the ETV system. Trace amounts of Fe, Cu and Ga could be directly determined in Al2O3 powders at the 2–270 μg g− 1 level without the use of thermochemical reagents. The results well agree with literature values for Fe and Cu, whereas deviations of 50% at the 90 μg g− 1 level for Ga were found.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3456141" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="25798f0e0597262174ff5dcbfcf36632" rel="nofollow" data-download="{"attachment_id":50275405,"asset_id":3456141,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50275405/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4037319" href="https://independent.academia.edu/RogerSperline">Roger Sperline</a><script data-card-contents-for-user="4037319" type="text/json">{"id":4037319,"first_name":"Roger","last_name":"Sperline","domain_name":"independent","page_name":"RogerSperline","display_name":"Roger Sperline","profile_url":"https://independent.academia.edu/RogerSperline?f_ri=5769","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_3456141 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3456141"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3456141, container: ".js-paper-rank-work_3456141", }); 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$(".js-view-count[data-work-id=3456141]").text(description); $(".js-view-count-work_3456141").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_3456141").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="3456141"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="5769" href="https://www.academia.edu/Documents/in/Mass_Spectrometry">Mass Spectrometry</a>, <script data-card-contents-for-ri="5769" type="text/json">{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="118582" href="https://www.academia.edu/Documents/in/Physical_sciences">Physical sciences</a>, <script data-card-contents-for-ri="118582" type="text/json">{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="222413" href="https://www.academia.edu/Documents/in/Inductively_Coupled_Plasma_Mass_Spectrometry">Inductively Coupled Plasma Mass Spectrometry</a>, <script data-card-contents-for-ri="222413" type="text/json">{"id":222413,"name":"Inductively Coupled Plasma Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Inductively_Coupled_Plasma_Mass_Spectrometry?f_ri=5769","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="260118" href="https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES">CHEMICAL SCIENCES</a><script data-card-contents-for-ri="260118" type="text/json">{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=5769","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=3456141]'), work: {"id":3456141,"title":"Electrothermal vaporization coupled with inductively coupled plasma array–detector mass spectrometry for the multielement analysis of Al 2O 3 ceramic powders","created_at":"2013-05-03T03:53:13.871-07:00","url":"https://www.academia.edu/3456141/Electrothermal_vaporization_coupled_with_inductively_coupled_plasma_array_detector_mass_spectrometry_for_the_multielement_analysis_of_Al_2O_3_ceramic_powders?f_ri=5769","dom_id":"work_3456141","summary":"An electrothermal vaporization (ETV) system useful for the analysis of solutions and slurries has been coupled with a sector-field inductively coupled plasma mass spectrometer (ICP–MS) equipped with an array detector. The ability of this instrument to record the transient signals produced for a number of analytes in ETV–ICP–MS is demonstrated. Detection limits for Mn, Fe, Co, Ni, Cu, Zn and Ga are in the range of 4–60 pg μL− 1 for aqueous solutions and in the low μg g− 1 range for the analysis of 10 mg mL− 1 slurries of Al2O3 powders. The dynamic ranges measured for Fe, Cu and Ga spanned 3–5 orders of magnitude when the detector was operated in the low-gain mode and appear to be limited by the ETV system. Trace amounts of Fe, Cu and Ga could be directly determined in Al2O3 powders at the 2–270 μg g− 1 level without the use of thermochemical reagents. The results well agree with literature values for Fe and Cu, whereas deviations of 50% at the 90 μg g− 1 level for Ga were found.","downloadable_attachments":[{"id":50275405,"asset_id":3456141,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4037319,"first_name":"Roger","last_name":"Sperline","domain_name":"independent","page_name":"RogerSperline","display_name":"Roger Sperline","profile_url":"https://independent.academia.edu/RogerSperline?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=5769","nofollow":false},{"id":222413,"name":"Inductively Coupled Plasma Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Inductively_Coupled_Plasma_Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=5769","nofollow":false},{"id":989646,"name":"Aqueous Solution","url":"https://www.academia.edu/Documents/in/Aqueous_Solution?f_ri=5769"},{"id":1826013,"name":"Mass Spectrometer","url":"https://www.academia.edu/Documents/in/Mass_Spectrometer?f_ri=5769"},{"id":1956581,"name":"Dynamic Range","url":"https://www.academia.edu/Documents/in/Dynamic_Range?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9518864" data-work_id="9518864" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9518864/Selective_binding_and_enrichment_for_low_molecular_weight_biomarker_molecules_in_human_plasma_after_exposure_to_nanoporous_silica_particles">Selective binding and enrichment for low-molecular weight biomarker molecules in human plasma after exposure to nanoporous silica particles</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The present manuscript describes a biomarker capturing strategy based on nanoporous silica particles. The method is shown to enrich the yield of species in the low-molecular weight proteome (LMWP), allowing detection of small peptides in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9518864" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The present manuscript describes a biomarker capturing strategy based on nanoporous silica particles. The method is shown to enrich the yield of species in the low-molecular weight proteome (LMWP), allowing detection of small peptides in the low-nanomolar range. Plasma samples were exposed to the silica particles, and the captured molecular species were profiled using MALDI-TOF. Mass spectra of the silica-treated human plasma samples showed a significant enrichment in MALDI-TOF protein profiles in the LMWP. Preliminary results indicated good level of reproducibility in plasma profiles with CVs on peak heights ranging from 6.3 to 14.7%. The MALDI-TOF signature changed significantly when the characteristics of the nanoporous silica were altered. The facile sample pretreatment before MS analysis, coupled to the potential for tailoring the surface properties of silica supports, hold promise for improving the recovery of low-abundance serum biomarkers.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9518864" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d82471b780d3664f4cfd7459e3820ade" rel="nofollow" data-download="{"attachment_id":47746088,"asset_id":9518864,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47746088/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="22341917" href="https://independent.academia.edu/FlavianoTesta">Flaviano Testa</a><script data-card-contents-for-user="22341917" type="text/json">{"id":22341917,"first_name":"Flaviano","last_name":"Testa","domain_name":"independent","page_name":"FlavianoTesta","display_name":"Flaviano Testa","profile_url":"https://independent.academia.edu/FlavianoTesta?f_ri=5769","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_9518864 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9518864"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9518864, container: ".js-paper-rank-work_9518864", }); 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The method is shown to enrich the yield of species in the low-molecular weight proteome (LMWP), allowing detection of small peptides in the low-nanomolar range. Plasma samples were exposed to the silica particles, and the captured molecular species were profiled using MALDI-TOF. Mass spectra of the silica-treated human plasma samples showed a significant enrichment in MALDI-TOF protein profiles in the LMWP. Preliminary results indicated good level of reproducibility in plasma profiles with CVs on peak heights ranging from 6.3 to 14.7%. The MALDI-TOF signature changed significantly when the characteristics of the nanoporous silica were altered. The facile sample pretreatment before MS analysis, coupled to the potential for tailoring the surface properties of silica supports, hold promise for improving the recovery of low-abundance serum biomarkers.","downloadable_attachments":[{"id":47746088,"asset_id":9518864,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":22341917,"first_name":"Flaviano","last_name":"Testa","domain_name":"independent","page_name":"FlavianoTesta","display_name":"Flaviano Testa","profile_url":"https://independent.academia.edu/FlavianoTesta?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5769,"name":"Mass 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Biology","url":"https://www.academia.edu/Documents/in/Computational_Biology?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":6779,"name":"Science","url":"https://www.academia.edu/Documents/in/Science?f_ri=5769"},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=5769"},{"id":59690,"name":"Bees","url":"https://www.academia.edu/Documents/in/Bees?f_ri=5769"},{"id":62285,"name":"Neuropeptides","url":"https://www.academia.edu/Documents/in/Neuropeptides?f_ri=5769"},{"id":64753,"name":"Apis mellifera","url":"https://www.academia.edu/Documents/in/Apis_mellifera?f_ri=5769"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=5769"},{"id":181936,"name":"Gene","url":"https://www.academia.edu/Documents/in/Gene?f_ri=5769"},{"id":316015,"name":"Gen","url":"https://www.academia.edu/Documents/in/Gen?f_ri=5769"},{"id":382393,"name":"Neuropeptide Y","url":"https://www.academia.edu/Documents/in/Neuropeptide_Y?f_ri=5769"},{"id":717335,"name":"Codon","url":"https://www.academia.edu/Documents/in/Codon?f_ri=5769"},{"id":732028,"name":"Genome sequence","url":"https://www.academia.edu/Documents/in/Genome_sequence?f_ri=5769"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=5769"},{"id":981291,"name":"Brain 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href="https://www.academia.edu/23855665/Structural_characterization_of_amyloid_fibrils_from_the_human_parathyroid_hormone">Structural characterization of amyloid fibrils from the human parathyroid hormone</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Amyloid deposits are common in various tissues as a consequence of misfolded proteins. However, secretory protein and peptides are often stored in membrane coated granules as functional amyloids. In this article, we present a detailed... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23855665" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Amyloid deposits are common in various tissues as a consequence of misfolded proteins. However, secretory protein and peptides are often stored in membrane coated granules as functional amyloids. In this article, we present a detailed characterization of in vitro generated amyloid fibrils from human parathyroid hormone (hPTH(1-84)). Fully mature fibrils could be obtained after a short lag phase within less than one hour at 65°C. These fibrils showed all characteristic of a cross-β structure. Protease cleavage combined with mass spectrometry identified the central region of the peptide hormone involved in the fibril core formation. EGCG, an inhibitor of amyloid fibril formation, showed binding to residues in the peptide monomers corresponding to the later fibril core and thus explaining the inhibition of the fibril growth. Conformational and dynamic studies by solid-state NMR further corroborated the cross-β core of the fibrils, but also identified highly mobile segments with a rando...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23855665" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3ccd85a73654f02583bc18b5ddcde6c1" rel="nofollow" data-download="{"attachment_id":44250091,"asset_id":23855665,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44250091/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="16011424" href="https://bharathuniv.academia.edu/SenthilKumar">Senthil Kumar</a><script data-card-contents-for-user="16011424" type="text/json">{"id":16011424,"first_name":"Senthil","last_name":"Kumar","domain_name":"bharathuniv","page_name":"SenthilKumar","display_name":"Senthil Kumar","profile_url":"https://bharathuniv.academia.edu/SenthilKumar?f_ri=5769","photo":"https://0.academia-photos.com/16011424/6023970/17738911/s65_senthil.kumar.jpg"}</script></span></span></li><li class="js-paper-rank-work_23855665 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23855665"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23855665, container: ".js-paper-rank-work_23855665", }); 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Conformational and dynamic studies by solid-state NMR further corroborated the cross-β core of the fibrils, but also identified highly mobile segments with a rando...","downloadable_attachments":[{"id":44250091,"asset_id":23855665,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":16011424,"first_name":"Senthil","last_name":"Kumar","domain_name":"bharathuniv","page_name":"SenthilKumar","display_name":"Senthil Kumar","profile_url":"https://bharathuniv.academia.edu/SenthilKumar?f_ri=5769","photo":"https://0.academia-photos.com/16011424/6023970/17738911/s65_senthil.kumar.jpg"}],"research_interests":[{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":40539,"name":"Amyloid","url":"https://www.academia.edu/Documents/in/Amyloid?f_ri=5769","nofollow":false},{"id":47884,"name":"Biological 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Their... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15063137" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The chromatographic separation of a methylene chloride extract of Artemisia rubripes led to the isolation of a new sesquiterpene lactone (3), together with four known compounds, a coumarin (2) and three terpenes (1, 4, and 5). Their structures were characterized to be 1beta,6alpha-dihydroxy-4(15)-eudesmene (1), scopoletin (2), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,10(14), 11(13)-triene-6,12-olide (3), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,9,11(13)-triene-6,12-olide (4), and beta-sitosterol-3-O-beta-D-glycoside (5) by spectroscopic means.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15063137" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="acdf193fdfb88c3c8568391c8724f2c8" rel="nofollow" data-download="{"attachment_id":43608977,"asset_id":15063137,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43608977/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34089244" href="https://independent.academia.edu/kyulee3">kyu lee</a><script data-card-contents-for-user="34089244" type="text/json">{"id":34089244,"first_name":"kyu","last_name":"lee","domain_name":"independent","page_name":"kyulee3","display_name":"kyu lee","profile_url":"https://independent.academia.edu/kyulee3?f_ri=5769","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15063137 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15063137"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15063137, container: ".js-paper-rank-work_15063137", }); 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Their structures were characterized to be 1beta,6alpha-dihydroxy-4(15)-eudesmene (1), scopoletin (2), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,10(14), 11(13)-triene-6,12-olide (3), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,9,11(13)-triene-6,12-olide (4), and beta-sitosterol-3-O-beta-D-glycoside (5) by spectroscopic means.","downloadable_attachments":[{"id":43608977,"asset_id":15063137,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34089244,"first_name":"kyu","last_name":"lee","domain_name":"independent","page_name":"kyulee3","display_name":"kyu lee","profile_url":"https://independent.academia.edu/kyulee3?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":21732,"name":"Magnetic Resonance Spectroscopy","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy?f_ri=5769","nofollow":false},{"id":156973,"name":"Artemisia","url":"https://www.academia.edu/Documents/in/Artemisia?f_ri=5769","nofollow":false},{"id":165799,"name":"Terpenes","url":"https://www.academia.edu/Documents/in/Terpenes?f_ri=5769"},{"id":239046,"name":"Sesquiterpenes","url":"https://www.academia.edu/Documents/in/Sesquiterpenes?f_ri=5769"},{"id":325790,"name":"Coumarins","url":"https://www.academia.edu/Documents/in/Coumarins?f_ri=5769"},{"id":326380,"name":"Liquid Chromatography / Electrospray Ionization Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography_Electrospray_Ionization_Mass_Spectrometry?f_ri=5769"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=5769"},{"id":1270314,"name":"Lactones","url":"https://www.academia.edu/Documents/in/Lactones?f_ri=5769"},{"id":1745595,"name":"Solvents","url":"https://www.academia.edu/Documents/in/Solvents?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22961418" data-work_id="22961418" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22961418/Thermal_decomposition_and_kinetic_analysis_of_sodium_propoxides">Thermal decomposition and kinetic analysis of sodium propoxides</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22961418" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f19d4e1bebe4fb32b44e82cc0bca3bbe" rel="nofollow" data-download="{"attachment_id":43482868,"asset_id":22961418,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43482868/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44665398" href="https://independent.academia.edu/KChandran1">K. 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Relationship","url":"https://www.academia.edu/Documents/in/Structure_activity_Relationship?f_ri=5769"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_79937970" data-work_id="79937970" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/79937970/Prebiotic_chemicals_amino_acid_and_phosphorus_in_the_coma_of_comet_67P_Churyumov_Gerasimenko">Prebiotic chemicals-amino acid and phosphorus-in the coma of comet 67P/Churyumov-Gerasimenko</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The importance of comets for the origin of life on Earth has been advocated for many decades. Amino acids are key ingredients in chemistry, leading to life as we know it. Many primitive meteorites contain amino acids, and it is generally... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_79937970" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The importance of comets for the origin of life on Earth has been advocated for many decades. Amino acids are key ingredients in chemistry, leading to life as we know it. Many primitive meteorites contain amino acids, and it is generally believed that these are formed by aqueous alterations. In the collector aerogel and foil samples of the Stardust mission after the flyby at comet Wild 2, the simplest form of amino acids, glycine, has been found together with precursor molecules methylamine and ethylamine. Because of contamination issues of the samples, a cometary origin was deduced from the (13)C isotopic signature. We report the presence of volatile glycine accompanied by methylamine and ethylamine in the coma of 67P/Churyumov-Gerasimenko measured by the ROSINA (Rosetta Orbiter Spectrometer for Ion and Neutral Analysis) mass spectrometer, confirming the Stardust results. Together with the detection of phosphorus and a multitude of organic molecules, this result demonstrates that c...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/79937970" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1c4e85c7c12224133ad75f7237489f35" rel="nofollow" data-download="{"attachment_id":86486262,"asset_id":79937970,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/86486262/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="31695927" href="https://unibe-ch.academia.edu/KathrinAltwegg">Kathrin Altwegg</a><script data-card-contents-for-user="31695927" type="text/json">{"id":31695927,"first_name":"Kathrin","last_name":"Altwegg","domain_name":"unibe-ch","page_name":"KathrinAltwegg","display_name":"Kathrin Altwegg","profile_url":"https://unibe-ch.academia.edu/KathrinAltwegg?f_ri=5769","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_79937970 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="79937970"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 79937970, container: ".js-paper-rank-work_79937970", }); 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Amino acids are key ingredients in chemistry, leading to life as we know it. Many primitive meteorites contain amino acids, and it is generally believed that these are formed by aqueous alterations. In the collector aerogel and foil samples of the Stardust mission after the flyby at comet Wild 2, the simplest form of amino acids, glycine, has been found together with precursor molecules methylamine and ethylamine. Because of contamination issues of the samples, a cometary origin was deduced from the (13)C isotopic signature. We report the presence of volatile glycine accompanied by methylamine and ethylamine in the coma of 67P/Churyumov-Gerasimenko measured by the ROSINA (Rosetta Orbiter Spectrometer for Ion and Neutral Analysis) mass spectrometer, confirming the Stardust results. Together with the detection of phosphorus and a multitude of organic molecules, this result demonstrates that c...","downloadable_attachments":[{"id":86486262,"asset_id":79937970,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":31695927,"first_name":"Kathrin","last_name":"Altwegg","domain_name":"unibe-ch","page_name":"KathrinAltwegg","display_name":"Kathrin Altwegg","profile_url":"https://unibe-ch.academia.edu/KathrinAltwegg?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=5769","nofollow":false},{"id":498,"name":"Physics","url":"https://www.academia.edu/Documents/in/Physics?f_ri=5769","nofollow":false},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":6779,"name":"Science","url":"https://www.academia.edu/Documents/in/Science?f_ri=5769"},{"id":18329,"name":"Prebiotics","url":"https://www.academia.edu/Documents/in/Prebiotics?f_ri=5769"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=5769"},{"id":47599,"name":"Astronomy","url":"https://www.academia.edu/Documents/in/Astronomy?f_ri=5769"},{"id":53108,"name":"Phosphorus","url":"https://www.academia.edu/Documents/in/Phosphorus?f_ri=5769"},{"id":295928,"name":"Amino Acids","url":"https://www.academia.edu/Documents/in/Amino_Acids?f_ri=5769"},{"id":889452,"name":"Meteoroids","url":"https://www.academia.edu/Documents/in/Meteoroids?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_72424642" data-work_id="72424642" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/72424642/Teicoplanin_antibiotics_from_Actinoplanes_teichomyceticus_nov_sp_V_Aromatic_constituents">Teicoplanin, antibiotics from Actinoplanes teichomyceticus nov. sp. 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Carbon","url":"https://www.academia.edu/Documents/in/Elemental_Carbon?f_ri=5769"},{"id":1707373,"name":"Mass Balance","url":"https://www.academia.edu/Documents/in/Mass_Balance?f_ri=5769"},{"id":1768455,"name":"Asian Dust","url":"https://www.academia.edu/Documents/in/Asian_Dust?f_ri=5769"},{"id":2631981,"name":"Troposphere","url":"https://www.academia.edu/Documents/in/Troposphere?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62960912" data-work_id="62960912" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62960912/Incorporation_of_the_Food_Mutagen_2_Amino_1_Methyl_6_Phenylimidazo_4_5_b_Pyridine_PhIP_into_Fur_and_Correlation_with_Intestinal_Tumourigenesis_in_Min_Mice">Incorporation of the Food Mutagen 2‐Amino‐1‐Methyl‐6‐Phenylimidazo [4, 5‐b] Pyridine (PhIP) into Fur and Correlation with Intestinal Tumourigenesis in Min/+ Mice</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Abstract: The purpose of this work was to correlate the amount of the food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) determined in mouse fur with the number of intestinal tumours induced by PhIP, to further evaluate... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_62960912" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Abstract: The purpose of this work was to correlate the amount of the food mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) determined in mouse fur with the number of intestinal tumours induced by PhIP, to further evaluate incorporation of PhIP into hair as 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class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/59150826/Pharmaceutical_profiling_method_for_lipophilicity_and_integrity_using_liquid_chromatography_mass_spectrometry">Pharmaceutical profiling method for lipophilicity and integrity using liquid chromatography–mass spectrometry</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/59150826" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a5f9d55f05a1b919c15ab9c4fc5d8474" rel="nofollow" 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{"id":59150826,"title":"Pharmaceutical profiling method for lipophilicity and integrity using liquid chromatography–mass spectrometry","created_at":"2021-10-20T13:20:40.574-07:00","url":"https://www.academia.edu/59150826/Pharmaceutical_profiling_method_for_lipophilicity_and_integrity_using_liquid_chromatography_mass_spectrometry?f_ri=5769","dom_id":"work_59150826","summary":null,"downloadable_attachments":[{"id":73222543,"asset_id":59150826,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":58175930,"first_name":"Edward","last_name":"Kerns","domain_name":"independent","page_name":"KernsEdward","display_name":"Edward Kerns","profile_url":"https://independent.academia.edu/KernsEdward?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass 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Compound","url":"https://www.academia.edu/Documents/in/Organic_Compound?f_ri=5769"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=5769"},{"id":2380004,"name":"Pharmaceutical preparations","url":"https://www.academia.edu/Documents/in/Pharmaceutical_preparations?f_ri=5769"},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_58685915" data-work_id="58685915" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/58685915/Usefulness_of_multilocus_polymerase_chain_reaction_followed_by_electrospray_ionization_mass_spectrometry_to_identify_a_diverse_panel_of_bacterial_isolates">Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_58685915" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/58685915" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cba92d02a2d2140dc28b152dc466824f" rel="nofollow" data-download="{"attachment_id":72976716,"asset_id":58685915,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72976716/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="25381401" href="https://independent.academia.edu/RangaSampath">Ranga Sampath</a><script data-card-contents-for-user="25381401" type="text/json">{"id":25381401,"first_name":"Ranga","last_name":"Sampath","domain_name":"independent","page_name":"RangaSampath","display_name":"Ranga Sampath","profile_url":"https://independent.academia.edu/RangaSampath?f_ri=5769","photo":"https://0.academia-photos.com/25381401/73984592/62474411/s65_ranga.sampath.jpeg"}</script></span></span></li><li class="js-paper-rank-work_58685915 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="58685915"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 58685915, container: ".js-paper-rank-work_58685915", }); 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Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.","downloadable_attachments":[{"id":72976716,"asset_id":58685915,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":25381401,"first_name":"Ranga","last_name":"Sampath","domain_name":"independent","page_name":"RangaSampath","display_name":"Ranga Sampath","profile_url":"https://independent.academia.edu/RangaSampath?f_ri=5769","photo":"https://0.academia-photos.com/25381401/73984592/62474411/s65_ranga.sampath.jpeg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":6947,"name":"Medical 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Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":54344,"name":"Methods","url":"https://www.academia.edu/Documents/in/Methods?f_ri=5769","nofollow":false},{"id":152519,"name":"Neisseria","url":"https://www.academia.edu/Documents/in/Neisseria?f_ri=5769"},{"id":383698,"name":"Leptospira","url":"https://www.academia.edu/Documents/in/Leptospira?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_54946661" data-work_id="54946661" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/54946661/The_high_mannose_oligosaccharides_of_Dictyostelium_discoideum_glycoproteins_contain_a_novel_intersecting_N_acetylglucosamine_residue">The high mannose oligosaccharides of Dictyostelium discoideum glycoproteins contain a novel intersecting N-acetylglucosamine residue</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Previous studies have shown that the majority of the asparagine-linked high mannose oligosaccharide units present on the lysosomal enzymes of the slime mold Dictyostelium discoideum contain mannose 6-sulfate and mannose 6-phosphate... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_54946661" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Previous studies have shown that the majority of the asparagine-linked high mannose oligosaccharide units present on the lysosomal enzymes of the slime mold Dictyostelium discoideum contain mannose 6-sulfate and mannose 6-phosphate residues, the latter being diesterified to methyl groups. Using a variety of techniques, including NMR spectroscopy and fast atom bombardment mass spectrometry, we have found that many of these oligosaccharides also contain an N-acetylglucosamine residue linked beta 1-4 to the mannose residue linked alpha 1-6 to the beta-linked core mannose. (Formula: see text) As far as we are aware, this is the first description of an intersecting N-acetylglucosamine residue in this position on a high mannose oligosaccharide.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/54946661" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5a84730bfb6e62fdcf07876320201be9" rel="nofollow" data-download="{"attachment_id":71059551,"asset_id":54946661,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/71059551/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32264225" href="https://independent.academia.edu/VernonReinhold">Vernon Reinhold</a><script data-card-contents-for-user="32264225" type="text/json">{"id":32264225,"first_name":"Vernon","last_name":"Reinhold","domain_name":"independent","page_name":"VernonReinhold","display_name":"Vernon Reinhold","profile_url":"https://independent.academia.edu/VernonReinhold?f_ri=5769","photo":"https://0.academia-photos.com/32264225/18837599/18796605/s65_vernon.reinhold.jpg"}</script></span></span></li><li class="js-paper-rank-work_54946661 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="54946661"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 54946661, container: ".js-paper-rank-work_54946661", }); 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Using a variety of techniques, including NMR spectroscopy and fast atom bombardment mass spectrometry, we have found that many of these oligosaccharides also contain an N-acetylglucosamine residue linked beta 1-4 to the mannose residue linked alpha 1-6 to the beta-linked core mannose. (Formula: see text) As far as we are aware, this is the first description of an intersecting N-acetylglucosamine residue in this position on a high mannose oligosaccharide.","downloadable_attachments":[{"id":71059551,"asset_id":54946661,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32264225,"first_name":"Vernon","last_name":"Reinhold","domain_name":"independent","page_name":"VernonReinhold","display_name":"Vernon Reinhold","profile_url":"https://independent.academia.edu/VernonReinhold?f_ri=5769","photo":"https://0.academia-photos.com/32264225/18837599/18796605/s65_vernon.reinhold.jpg"}],"research_interests":[{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=5769","nofollow":false},{"id":18520,"name":"Biological Chemistry","url":"https://www.academia.edu/Documents/in/Biological_Chemistry?f_ri=5769","nofollow":false},{"id":21732,"name":"Magnetic Resonance Spectroscopy","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy?f_ri=5769","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=5769","nofollow":false},{"id":170274,"name":"Dictyostelium","url":"https://www.academia.edu/Documents/in/Dictyostelium?f_ri=5769"},{"id":191439,"name":"Glycoproteins","url":"https://www.academia.edu/Documents/in/Glycoproteins?f_ri=5769"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=5769"},{"id":1032192,"name":"Mannose","url":"https://www.academia.edu/Documents/in/Mannose?f_ri=5769"},{"id":1974871,"name":"Oligosaccharides","url":"https://www.academia.edu/Documents/in/Oligosaccharides?f_ri=5769"},{"id":2285372,"name":"N-acetylglucosamine","url":"https://www.academia.edu/Documents/in/N-acetylglucosamine?f_ri=5769"},{"id":2467564,"name":"Carbohydrate Sequence","url":"https://www.academia.edu/Documents/in/Carbohydrate_Sequence?f_ri=5769"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_50420717" data-work_id="50420717" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/50420717/Occurrence_of_natural_fullerenes_in_low_grade_metamorphosed_Proterozoic_shungite_from_Karelia_Russia">Occurrence of natural fullerenes in low grade metamorphosed Proterozoic shungite from Karelia, Russia</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/50420717" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6f2a7771cc59f17ecbc66a3f442b6371" rel="nofollow" data-download="{"attachment_id":68415722,"asset_id":50420717,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/68415722/download_file?st=MTczMjQxMzIyNyw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50066378" href="https://independent.academia.edu/MVairamani">M. 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biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=5769"},{"id":2535440,"name":"Paroxetine","url":"https://www.academia.edu/Documents/in/Paroxetine?f_ri=5769"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_3981349" data-work_id="3981349" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/3981349/Development_and_validation_of_a_sensitive_HPLC_ESI_MS_MS_method_for_the_direct_determination_of_glucosamine_in_human_plasma">Development and validation of a sensitive HPLC–ESI-MS/MS method for the direct determination of glucosamine in human plasma</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A sensitive and specific HPLC–ESI-MS/MS method for the direct determination of glucosamine in human plasma has been developed and validated. Plasma samples were analyzed after a simple, one-step protein precipitation clean-up with... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_3981349" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A sensitive and specific HPLC–ESI-MS/MS method for the direct determination of glucosamine in human plasma has been developed and validated. Plasma samples were analyzed after a simple, one-step protein precipitation clean-up with trichloroacetic acid using a polymer-based amino high-performance liquid chromatography (HPLC) column and a water/acetonitrile mobile phase elution gradient, with d-[1-13C]glucosamine as the internal standard. Detection was performed by mass spectrometry, using an electrospray source and employing multiple reaction monitoring to separately monitor glucosamine and the internal standard. The limit of quantification of the method was 10 ng/ml of glucosamine and the calibration curve showed a good linearity up to 1000 ng/ml. The precision (R.S.D.) and the accuracy (bias) of the method at the limit of quantification were 13.8 and 4.0%, respectively, and the mean recovery of glucosamine at three concentration levels was 101.6 ± 5.7%. The method was applied for the determination of glucosamine concentrations in human plasma samples collected from untreated healthy volunteers and, in a separate bioavailability study, to evaluate plasma glucosamine pharmacokinetics profiles after oral administration of crystalline glucosamine sulfate.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/3981349" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8da03ea1840cf02a444d720d89e2df16" rel="nofollow" data-download="{"attachment_id":50100349,"asset_id":3981349,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50100349/download_file?st=MTczMjQxMzIyOCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4784101" href="https://independent.academia.edu/francescoviolante">francesco violante</a><script data-card-contents-for-user="4784101" type="text/json">{"id":4784101,"first_name":"francesco","last_name":"violante","domain_name":"independent","page_name":"francescoviolante","display_name":"francesco violante","profile_url":"https://independent.academia.edu/francescoviolante?f_ri=5769","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_3981349 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="3981349"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 3981349, container: ".js-paper-rank-work_3981349", }); 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Plasma samples were analyzed after a simple, one-step protein precipitation clean-up with trichloroacetic acid using a polymer-based amino high-performance liquid chromatography (HPLC) column and a water/acetonitrile mobile phase elution gradient, with d-[1-13C]glucosamine as the internal standard. Detection was performed by mass spectrometry, using an electrospray source and employing multiple reaction monitoring to separately monitor glucosamine and the internal standard. The limit of quantification of the method was 10 ng/ml of glucosamine and the calibration curve showed a good linearity up to 1000 ng/ml. The precision (R.S.D.) and the accuracy (bias) of the method at the limit of quantification were 13.8 and 4.0%, respectively, and the mean recovery of glucosamine at three concentration levels was 101.6 ± 5.7%. The method was applied for the determination of glucosamine concentrations in human plasma samples collected from untreated healthy volunteers and, in a separate bioavailability study, to evaluate plasma glucosamine pharmacokinetics profiles after oral administration of crystalline glucosamine sulfate.","downloadable_attachments":[{"id":50100349,"asset_id":3981349,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4784101,"first_name":"francesco","last_name":"violante","domain_name":"independent","page_name":"francescoviolante","display_name":"francesco violante","profile_url":"https://independent.academia.edu/francescoviolante?f_ri=5769","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":524,"name":"Analytical Chemistry","url":"https://www.academia.edu/Documents/in/Analytical_Chemistry?f_ri=5769","nofollow":false},{"id":5769,"name":"Mass 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